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https://www.readbyqxmd.com/read/29220418/plant-mediated-female-transcriptomic-changes-post-mating-in-a-tephritid-fruit-fly-bactrocera-tryoni
#1
Kumaran Nagalingam, Chloé A van der Burg, Yujia Qin, Stephen L Cameron, Anthony R Clarke, Peter J Prentis
Female post-mating behaviours are regulated by complex factors involving males, females and the environment. In insects, plant secondary compounds that males actively forage for, may indirectly modify female behaviours by altering male behaviour and physiology. In the tephritid fruit fly, Bactrocera tryoni, females mated with males previously fed on plant-derived phenylpropanoids (= 'lures' based on usage in tephritid literature), have longer mating refractoriness, greater fecundity and reduced longevity than females mated with non-lure fed males...
December 6, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/29219187/integrated-molecular-biochemical-and-physiological-assessment-unravels-key-extraction-method-mediated-influences-on-rat-neonatal-cardiomyocytes
#2
Leonardo Jensen, Elida Neri, Vinicius Bassaneze, Nathalia Corrêa De Almeida Oliveira, Rafael Dariolli, Thiago Lauro Turaça, Débora Levy, Douglas Veronez, Mariana Sacrini Ayres Ferraz, Adriano Mesquita Alencar, Sérgio Paulo Bydlowski, Idágene Aparecida Cestari, José Eduardo Krieger
Neonatal cardiomyocytes are instrumental for disease modeling, but the effects of different cell extraction methods on basic cell biological processes remain poorly understood. We assessed the influence of two popular methods to extract rat neonatal cardiomyocytes, Pre-plating (PP) and Percoll (PC) on cell structure, metabolism, and function. Cardiomyocytes obtained from PP showed higher gene expression for troponins, titin, and potassium and sodium channels compared to PC. Also, PP cells displayed higher levels of troponin I protein...
December 8, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29212849/trim63-murf-1-gene-polymorphism-is-associated-with-biomarkers-of-exercise-induced-muscle-damage
#3
Philipp Baumert, Mark J Lake, Barry Drust, Claire Stewart, Robert M Erskine
Unaccustomed strenuous exercise can lead to muscle strength loss, inflammation and delayed onset muscle soreness, which may be influenced by genetic variation. We investigated if a missense single nucleotide polymorphism (A>G, rs2275950) within the TRIM63 gene (encoding MuRF-1 and potentially affecting titin mechanical properties) was associated with the variable response to unaccustomed eccentric exercise. Sixty-five untrained, healthy participants (genotyped for rs2275950: AA, AG and GG) performed 120 maximal eccentric knee extensions (ECC) to induce muscle damage...
December 6, 2017: Physiological Genomics
https://www.readbyqxmd.com/read/29203134/proteomic-profiling-of-large-myofibrillar-proteins-from-dried-and-long-term-stored-polyacrylamide-gels
#4
Sandra Murphy, Kay Ohlendieck
A method for the utilization of dried polyacrylamide gels from the pre-proteomic era is described in order to enable the mass spectrometric analysis of long-term stored protein preparations. The in-gel digestion of high-molecular-mass proteins embedded in a 20-year old gel was carried out following gel re-swelling and resulted in the proteomic identification of a large number of proteins, including 3400 kDa titin, 800 kDa nebulin and myosin heavy chains of 220 kDa from rabbit skeletal muscle. These findings demonstrate that dried protein gels from past biochemical analyses can be successfully reused and analyzed by modern and refined mass spectrometric techniques...
December 1, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/29197093/the-association-between-irisin-and-muscle-metabolism-in-different-thyroid-disorders
#5
Ariadna Zybek-Kocik, Nadia Sawicka-Gutaj, Ewelina Szczepanek-Parulska, Mirosław Andrusiewicz, Joanna Waligórska-Stachura, Piotr Białas, Tomasz Krauze, Przemysław Guzik, Jerzy Skrobisz, Marek Ruchała
BACKGROUND: Irisin is a new adipo-myokine, encoded by the FNDC5 gene. Currently, there is a discussion regarding the relation between thyroid function and irisin concentration. This prospective study assesses the influence of thyrometabolic changes on serum irisin concentration in association with altered muscle metabolism. This is performed on a large cohort of patients affected by severe hypo- or hyperthyroidism, as well as by the expression of the FNDC5 gene in thyroid tissue affected by different pathologies...
December 2, 2017: Clinical Endocrinology
https://www.readbyqxmd.com/read/29193283/diastolic-dysfunction-in-pulmonary-artery-hypertension-creatine-kinase-and-the-potential-therapeutic-benefit-of-beta-blockers
#6
Ewan D Fowler, Mark J Drinkhill, Rachel Stones, Ed White
Passive properties of the myocardium influence diastolic filling and cardiac output. In heart failure, changes in contributors to the passive properties of the ventricle, such as titin and collagen, and loss of the metabolic enzyme creatine kinase, increase resistance to filling resulting in diastolic dysfunction. Pulmonary artery hypertension (PAH) arises from interactions between the pulmonary vasculature and the right ventricle (RV) which ultimately leads to RV failure. Beta1-adrenergic receptor blockers (BB) act on the myocardium and are beneficial in left heart failure but are not used in PAH...
November 28, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/29190010/are-mechanically-sensitive-regulators-involved-in-the-function-and-patho-physiology-of-cerebral-palsy-related-contractures
#7
Jessica Pingel, Frank Suhr
Skeletal muscle tissue is mechanosensitive, as it is able to sense mechanical impacts and to translate these into biochemical signals making the tissue adapt. Among its mechanosensitive nature, skeletal muscle tissue is the largest metabolic organ of the human body. Disturbances in skeletal muscle mechanosensing and metabolism cause and contribute to many diseases, i.e. muscular dystrophies/myopathies, cardiovascular diseases, COPD or diabetes mellitus type 2. A less commonly focused muscle-related disorder is clinically known as muscle contractures that derive from cerebral palsy (CP) conditions in young and adults...
November 30, 2017: Journal of Muscle Research and Cell Motility
https://www.readbyqxmd.com/read/29175173/diagnostic-and-clinical-significance-of-the-titin-fragment-in-urine-of-duchenne-muscular-dystrophy-patients
#8
Hiroyuki Awano, Masaaki Matsumoto, Masashi Nagai, Taku Shirakawa, Nobuhiro Maruyama, Kazumoto Iijima, Yo-Ichi Nabeshima, Masafumi Matsuo
Duchenne muscular dystrophy (DMD) is a fatal progressive muscle wasting disease of childhood. Titin in sarcomere is digested by calcium dependent protease. To explore muscle damage in DMD, the urinary concentrations of the N-terminal fragment of titin were determined using a newly developed enzyme linked immune sorbent assay kit. The urinary titin concentrations were normalized to creatinine (Cr). A total of 145 urine samples were obtained at a single Japanese hospital from 113 DMD patients aged 3-29years. Normalized urinary titin concentration was 965...
November 23, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29168801/the-histone-code-reader-spin1-controls-skeletal-muscle-development
#9
Holger Greschik, Delphine Duteil, Nadia Messaddeq, Dominica Willmann, Laura Arrigoni, Manuela Sum, Manfred Jung, Daniel Metzger, Thomas Manke, Thomas Günther, Roland Schüle
While several studies correlated increased expression of the histone code reader Spin1 with tumor formation or growth, little is known about physiological functions of the protein. We generated Spin1(M5) mice with ablation of Spin1 in myoblast precursors using the Myf5-Cre deleter strain. Most Spin1(M5) mice die shortly after birth displaying severe sarcomere disorganization and necrosis. Surviving Spin1(M5) mice are growth-retarded and exhibit the most prominent defects in soleus, tibialis anterior, and diaphragm muscle...
November 23, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/29139381/-titin-related-muscle-disorders-an-expanding-spectrum
#10
Ana Ferreiro, J Andoni Urtizberea
Titin-related diseases of the skeletal and cardiac muscles open a new, fruitful chapter of myology. Confined for a long time to a limited number of clinical entities, the phenotypic spectrum of titinopthies is nowadays expanding rapidly together with the discovery of many pathogenic mutations of the TTN gene. Like for many genes of large size, the fine tuning and use of high-throughput sequencing (NGS) constitutes a little revolution in the field. This powerful tool allows, although with real technical hurdles, the establishment of the definite diagnosis of titinopathy...
November 2017: Médecine Sciences: M/S
https://www.readbyqxmd.com/read/29131758/titin-gene-and-protein-functions-in-passive-and-active-muscle
#11
Wolfgang A Linke
The thin and thick filaments of muscle sarcomeres are interconnected by the giant protein titin, which is a scaffolding filament, signaling platform, and provider of passive tension and elasticity in myocytes. This review summarizes recent insight into the mechanisms behind how titin gene mutations cause hereditary cardiomyopathy and how titin protein is mechanically active in skeletal and cardiac myocytes. A main theme is the evolving role of titin as a modulator of contraction. Topics include strain-sensing via titin in the sarcomeric A-band as the basis for length-dependent activation, titin elastic recoil and refolding of titin domains as an energy source, and Ca2+-dependent stiffening of titin stretched during eccentric muscle contractions...
November 13, 2017: Annual Review of Physiology
https://www.readbyqxmd.com/read/29119312/when-signalling-goes-wrong-pathogenic-variants-in-structural-and-signalling-proteins-causing-cardiomyopathies
#12
Mehroz Ehsan, He Jiang, Kate L Thomson, Katja Gehmlich
Cardiomyopathies are a diverse group of cardiac disorders with distinct phenotypes, depending on the proteins and pathways affected. A substantial proportion of cardiomyopathies are inherited and those will be the focus of this review article. With the wide application of high-throughput sequencing in the practice of clinical genetics, the roles of novel genes in cardiomyopathies are recognised. Here, we focus on a subgroup of cardiomyopathy genes [TTN, FHL1, CSRP3, FLNC and PLN, coding for Titin, Four and a Half LIM domain 1, Muscle LIM Protein, Filamin C and Phospholamban, respectively], which, despite their diverse biological functions, all have important signalling functions in the heart, suggesting that disturbances in signalling networks can contribute to cardiomyopathies...
November 8, 2017: Journal of Muscle Research and Cell Motility
https://www.readbyqxmd.com/read/29114382/the-inhibitory-effects-of-polysaccharide-peptides-psp-of-ganoderma-lucidum-against-atherosclerosis-in-rats-with-dyslipidemia
#13
Titin Andri Wihastuti, Teuku Heriansyah
Background: Atherosclerosis occurs as a result of low-density lipoprotein (LDL) deposits oxidation. Endothelial dysfunction is an early process of atherosclerosis. Restoring endothelial lining back to normal by endothelial progenitor cells (EPCs) is critical for slowing or reversing vascular disease progression. Oxidative stress from hydrogen peroxide (H2O2) is increased in dyslipidemia so that antioxidant agent is required to prevent destruction of blood vessels. Objectives: This study aims to report Ganoderma lucidum polysaccharide peptide (PsP) effects in atherogenic process by measuring H2O2 level, IL-10 level, and EPC number in blood serum, and also intima-media thickness of aorta in dyslipidemia Wistar rat model by giving them a hypercholesterol diet (HCD)...
January 2017: Heart International
https://www.readbyqxmd.com/read/29111540/transcription-and-regulation-of-hepatitis-b-virus-genes-in-host-sperm-cells
#14
Ying Zhong, Dong-Ling Liu, Mohamed Morsi M Ahmed, Peng-Hao Li, Xiao-Ling Zhou, Qing-Dong Xie, Xiao-Qing Xu, Ting-Ting Han, Zhi-Wei Hou, Ji-Hua Huang, Lan Xu, Tian-Hua Huang
To investigate whether transcription of hepatitis B virus (HBV) gene occurs in human sperm, total RNA was extracted from sperm of patients with chronic HBV infection (test-1), from donor sperm transfected with a plasmid containing the full-length HBV genome (test-2), and from nontransfected donor sperm (control), used as the template for reverse transcription-polymerase chain reaction (RT-PCR). Positive bands for HBV DNA were observed in the test groups but not in the control. Next, to identify the role of host genes in regulating viral gene transcription in sperm, total RNA was extracted from 2-cell embryos derived from hamster oocytes fertilized in vitro by HBV-transfected (test) or nontransfected (control) human sperm and successively subjected to SMART-PCR, suppression subtractive hybridization, T/A cloning, bacterial amplification, microarray hybridization, sequencing and the Basic Local Alignment Search Tool (BLAST) search to isolate differentially expressed genes...
November 7, 2017: Asian Journal of Andrology
https://www.readbyqxmd.com/read/29109008/the-pathogenic-gene-screening-in-a-chinese-familial-dilated-cardiomyopathy-pedigree-from-hubei
#15
Yongnan Lyu, Jingjing Chen, Hongxin Xu
Dilated cardiomyopathy arises from mutations in many genes. TTN, the gene encoding the sarcomere protein titin, has been insufficiently analyzed for cardiomyopathy mutations because of its enormous size. In this study, we report a Chinese family with two members affected by TTN. Blood samples were collected from all family members. Genomic DNA was isolated from blood, and all coding exons and adjacent intronic sequences of the TTN gene were examined for mutation analysis using polymerase chain reaction (PCR)-based sequencing...
November 3, 2017: Gene
https://www.readbyqxmd.com/read/29093449/abnormal-contractility-in-human-heart-myofibrils-from-patients-with-dilated-cardiomyopathy-due-to-mutations-in-ttn-and-contractile-protein-genes
#16
Petr G Vikhorev, Natalia Smoktunowicz, Alex B Munster, O'Neal Copeland, Sawa Kostin, Cecile Montgiraud, Andrew E Messer, Mohammad R Toliat, Amy Li, Cristobal G Dos Remedios, Sean Lal, Cheavar A Blair, Kenneth S Campbell, Maya Guglin, Ralph Knoll, Steven B Marston
Dilated cardiomyopathy (DCM) is an important cause of heart failure. Single gene mutations in at least 50 genes have been proposed to account for 25-50% of DCM cases and up to 25% of inherited DCM has been attributed to truncating mutations in the sarcomeric structural protein titin (TTNtv). Whilst the primary molecular mechanism of some DCM-associated mutations in the contractile apparatus has been studied in vitro and in transgenic mice, the contractile defect in human heart muscle has not been studied. In this study we isolated cardiac myofibrils from 3 TTNtv mutants, and 3 with contractile protein mutations (TNNI3 K36Q, TNNC1 G159D and MYH7 E1426K) and measured their contractility and passive stiffness in comparison with donor heart muscle as a control...
November 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29077808/titindb-a-computational-tool-to-assess-titin-s-role-as-a-disease-gene
#17
Anna Laddach, Mathias Gautel, Franca Fraternali
Summary: Large numbers of rare and unique titin missense variants have been discovered in both healthy and disease cohorts, thus the correct classification of variants as pathogenic or non-pathogenic has become imperative. Due to titin's large size (363 coding exons), current web applications are unable to map titin variants to domain structures. Here, we present a web application, TITINdb, which integrates titin structure, variant, sequence and isoform information, along with pre-computed predictions of the impact of non-synonymous single nucleotide variants, to facilitate the correct classification of titin variants...
November 1, 2017: Bioinformatics
https://www.readbyqxmd.com/read/29076275/involvement-of-%C3%AE-m-calpain-in-the-proteolysis-and-meat-quality-changes-during-postmortem-storage-of-chicken-breast-muscle
#18
Liang Zhao, Tong Xing, Jichao Huang, Yan Qiao, Yulian Chen, Ming Huang
The objective of this study was to investigate the role of calpain isotypes, especially poultry-specific μ/m-calpain in the proteolysis and meat quality changes of chicken breast muscle during postmortem storage. Calpain activity was detected by casein zymography, while the degradation of titin, desmin and Troponin-T was analyzed by sodium dodecyl sulfate - polyacrylamide gel electrophoresis and western blot. Meat quality indicators such as water holding capacity and tenderness were also studied. The correlation analysis between calpain activity, proteolysis and the changes in meat quality indicators indicated that there were strong correlations for μ-calpain during the first 12 h of storage, while such strong correlations for μ/m-calpain were only found in samples stored from 12 h to 7 days...
October 26, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/29073956/should-identifying-a-titin-truncating-variant-change-the-management-of-patients-with-dilated%C3%A2-cardiomyopathy
#19
EDITORIAL
Christopher Newton-Cheh
No abstract text is available yet for this article.
October 31, 2017: Journal of the American College of Cardiology
https://www.readbyqxmd.com/read/29073955/phenotype-and-clinical-outcomes-of-titin%C3%A2-cardiomyopathy
#20
Upasana Tayal, Simon Newsome, Rachel Buchan, Nicola Whiffin, Brian Halliday, Amrit Lota, Angharad Roberts, A John Baksi, Inga Voges, Will Midwinter, Alijca Wilk, Risha Govind, Roddy Walsh, Piers Daubeney, Julian W E Jarman, Resham Baruah, Michael Frenneaux, Paul J Barton, Dudley Pennell, James S Ware, Sanjay K Prasad, Stuart A Cook
BACKGROUND: Improved understanding of dilated cardiomyopathy (DCM) due to titin truncation (TTNtv) may help guide patient stratification. OBJECTIVES: The purpose of this study was to establish relationships among TTNtv genotype, cardiac phenotype, and outcomes in DCM. METHODS: In this prospective, observational cohort study, DCM patients underwent clinical evaluation, late gadolinium enhancement cardiovascular magnetic resonance, TTN sequencing, and adjudicated follow-up blinded to genotype for the primary composite endpoint of cardiovascular death, and major arrhythmic and major heart failure events...
October 31, 2017: Journal of the American College of Cardiology
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