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Epimedium flavanoids

Xue-Chao Lv, Liang-Jia Bi, Ying Jiang, Xiang Wang
Icariin (ICA), a flavanoid isolated from herbal Epimedium, has multiple biological activities. The present study investigated the effects of ICA on the proliferation and alkaline phosphatase (ALP) activity (an index for PDLC differentiation) of human periodontal ligament cells (hPDLCs) inhibited by lipopolysaccharide (LPS). hPDLCs were cultured in vitro and stimulated with various concentrations of ICA. The proliferation ability of hPDLCs was detected by an MTT assay. The activity of ALP was determined by the p-Nitrophenyl phosphate method, and the expression of ALP was analyzed by reverse transcription polymerase chain reaction and western blot analysis...
November 2013: Molecular Medicine Reports
Jian-Hua Huang, Zi-Yin Shen, Bin Wu
OBJECTIVE: To investigate the effect and its mechanism of Epimedium flavanoids (EF) in retarding aging with different systematic viewpoints. METHODS: Hypothalamus, pituitary, adrenal and lymphocytes taken from 4-, 10-, 18-, 24-month old rats and from EF treated 24-month old rats were used to measure whole genome mRNA expression by gene array. Serum samples were used for metabonomic assay with high performance liquid chromatography. Using specific gene chip for NF-kappaB signaling pathway to detect the gene expression of the molecule related to that pathway in lymphocytes...
January 2008: Chinese Journal of Integrated Traditional and Western Medicine
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