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Bacteriology

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https://www.readbyqxmd.com/read/28225901/tuberculosis-associated-factors-caused-by-mycobacterium-tuberculosis-of-the-rdrio-genotype
#1
Eloise Brasil Moraes, Letícia Slompo, Amanda Juliane Finardi, Heloisa Paro Pedro da Silveira, Luciana Ruiz, Harrison Magdinier Gomes, Virginia Bodelão Richini, Philip Suffys, Carlos Magno Castelo Branco Fortaleza, Ricardo Cavalcanti, Ida Maria Foschiani Dias Baptista
BACKGROUND: Tuberculosis (TB) continues to be a disease that affects many countries around the world, including Brazil. Recently, a subtype of Latin American-Mediterranean family strain was identified and characterised by RDRio. The strain has been associated with different characteristics of the disease. OBJECTIVES: In the present study we investigated the association of epidemiological, clinical, radiological and bacteriological variables with pulmonary tuberculosis caused by RDRioMycobacterium tuberculosis strain in large regions of São Paulo...
March 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28225895/tuberculosis-associated-factors-caused-by-mycobacterium-tuberculosis-of-the-rdrio-genotype
#2
Eloise Brasil Moraes, Letícia Slompo, Amanda Juliane Finardi, Heloisa Paro Pedro da Silveira, Luciana Ruiz, Harrison Magdinier Gomes, Virginia Bodelão Richini, Philip Suffys, Carlos Magno Castelo Branco Fortaleza, Ricardo Cavalcanti, Ida Maria Foschiani Dias Baptista
BACKGROUND: Tuberculosis (TB) continues to be a disease that affects many countries around the world, including Brazil. Recently, a subtype of Latin American-Mediterranean family strain was identified and characterised by RDRio. The strain has been associated with different characteristics of the disease. OBJECTIVES: In the present study we investigated the association of epidemiological, clinical, radiological and bacteriological variables with pulmonary tuberculosis caused by RDRioMycobacterium tuberculosis strain in large regions of São Paulo...
February 16, 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28224107/risk-factors-for-mortality-in-postoperative-peritonitis-in-critically-ill-patients
#3
Yoann Launey, Benjamin Duteurtre, Raphaëlle Larmet, Nicolas Nesseler, Audrey Tawa, Yannick Mallédant, Philippe Seguin
AIM: To identify the risk factors for mortality in intensive care patients with postoperative peritonitis (POP). METHODS: This was a retrospective analysis using a prospective database that includes all patients hospitalized in a surgical intensive care unit for POP from September 2006 to August 2011. The data collected included demographics, comorbidities, postoperative severity parameters, bacteriological findings, adequacy of antimicrobial therapy and surgical treatments...
February 4, 2017: World Journal of Critical Care Medicine
https://www.readbyqxmd.com/read/28221928/rapid-identification-of-shiga-toxin-producing-escherichia-coli-o-serogroups-from-fresh-produce-and-raw-milk-enrichment-cultures-by-luminex-bead-based-suspension-array
#4
Julie A Kase, Anna Maounounen-Laasri, Andrew Lin
The U.S. Food and Drug Administration's Bacteriological Analytical Manual (BAM) Chapter 4a describes a Luminex microbead-based suspension array used to screen colonies for 11 clinically relevant Shiga toxin-producing Escherichia coli (STEC) serogroups: O26, O45, O91, O103, O104, O111, O113, O121, O128, O145, and O157. We evaluated the usefulness of this method to identify STEC-positive enrichment samples before agar plating. Twelve E. coli strains were added to three types of fresh produce (bagged baby spinach, alfalfa sprouts, and cilantro) at levels near the detection limit of the test...
September 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221923/matrix-specific-method-validation-of-an-automated-most-probable-number-system-for-use-in-measuring-bacteriological-quality-of-grade-a-milk-products
#5
Samantha Lindemann, Matthew Kmet, Ravinder Reddy, Steffen Uhlig
The U.S. Food and Drug Administration (FDA) oversees a long-standing cooperative federal and state milk sanitation program that uses the grade "A" Pasteurized Milk Ordinance standards to maintain the safety of grade "A" milk sold in the United States. The Pasteurized Milk Ordinance requires that grade "A" milk samples be tested using validated total aerobic bacterial and coliform count methods. The objective of this project was to conduct an interlaboratory method validation study to compare performance of a film plate method with an automated most-probable-number method for total aerobic bacterial and coliform counts, using statistical approaches from international data standards...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221854/effects-of-fish-derived-biological-preservatives-on-cold-storage-of-grass-carp-ctenopharyngodon-idellus-fillets
#6
Tingting Li, Yang Jiang, Gaowei Jin, Qiancheng Zhao, Jianrong Li
Two kinds of fish-derived natural antimicrobial substances, protamine and catfish ( Clarias fuscus ) epidermal mucus extract (CEME), were evaluated for their effect on the quality of grass carp fillets. Fillets were atmosphere packaged in cast polypropylene bags and were stored at 4°C in a low-temperature incubator without light. Solutions (0.5%, wt/vol) of CEME and protamine were used for dip pretreatment, respectively. Bacteriological (aerobic plate count, Shewanella putrefaciens , Pseudomonas fluorescens ) and physicochemical (pH, total volatile basic nitrogen, K value, surface color, texture profiles, water distribution) parameters and volatile odor compounds were measured on days 0, 3, 6, 9, 12, and 15...
October 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28219366/bacteriology-of-the-conjunctiva-in-pre-cataract-surgery-patients-with-occluded-nasolacrimal-ducts-and-the-operation-outcomes-in-japanese-patients
#7
Yuko Hayashi, Takeshi Miyamoto, Shuko Fujita, Katsuo Tomoyose, Nobuyuki Ishikawa, Masahide Kokado, Takayoshi Sumioka, Yuka Okada, Shizuya Saika
BACKGROUND: Contamination of the conjunctiva in association with nasolacrimal duct obstruction is by all accounts a risk factor for infectious endophthalmitis post-cataract surgery. METHODS: All patients who underwent cataract day surgery routinely received nasolacrimal duct syringing with normal saline at the Wakayama Medical University Hospital, Japan, from 2011 to 2013. The microorganisms isolated from conjunctival swab samples of patients with occluded nasolacrimal ducts and their susceptibility to antibiotics, as well as the operation outcomes in all the patients were retrospectively investigated...
February 20, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28215893/short-communication-diagnostic-performance-of-on-farm-bacteriological-culture-systems-for-identification-of-uterine-escherichia-coli-in-postpartum-dairy-cows
#8
J Dubuc
The objectives of this study were to validate the performance of on-farm bacteriological culture systems for identification of Escherichia coli in the uterus of early postpartum dairy cows and to determine if an association is present between the results and the subsequent occurrence of puerperal metritis (PM). A prospective cohort study was conducted in one commercial Holstein dairy herd in which 400 cows were sampled between 24 and 48 h after parturition. Three bacteriological samples were obtained from the uterus of each cow and were cultured for identification of E...
February 16, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28214907/a-pilot-study-of-bacterial-regrowth-in-water-pipelines
#9
Laura Mancini, Stefania Marcheggiani, Cinzia Grasso, Cristina Romanelli, Antonio Mistretta, Marina Marranzano
Bacterial regrowth in water distribution systems results in deterioration of bacteriological quality of drinking water, as well as accelerated corrosion of the pipelines. The aim of the present study was to analyze the phenomena of colonization and bacterial regrowth in source water and in the water distribution systems of three distribution networks in the province of Catania (Sicily, Italy). The BART™ (Biological Activity Reaction Test) method was used, which is also capable of determining the potential aggressiveness of microbial species in water...
November 2016: Igiene e Sanità Pubblica
https://www.readbyqxmd.com/read/28212322/detection-of-salmonella-typhimurium-on-spinach-using-phage-based-magnetoelastic-biosensors
#10
Fengen Wang, Shin Horikawa, Jiajia Hu, Howard C Wikle, I-Hsuan Chen, Songtao Du, Yuzhe Liu, Bryan A Chin
Phage-based magnetoelastic (ME) biosensors have been studied as an in-situ, real-time, wireless, direct detection method of foodborne pathogens in recent years. This paper investigates an ME biosensor method for the detection of Salmonella Typhimurium on fresh spinach leaves. A procedure to obtain a concentrated suspension of Salmonella from contaminated spinach leaves is described that is based on methods outlined in the U.S. FDA Bacteriological Analytical Manual for the detection of Salmonella on leafy green vegetables...
February 16, 2017: Sensors
https://www.readbyqxmd.com/read/28208889/management-of-pneumonia-and-blood-stream-infections-with-new-antibiotic-adjuvant-entity-ceftriaxone-sulbactam-disodium-edetate-a-novel-way-to-spare-carbapenems
#11
Mohammad Shameem, Mohammad Amin Mir
INTRODUCTION: Nosocomial infections have been considered as a major health problem causing incremental morbidity, mortality and costs of therapy. AIM: This retrospective study was initiated with aim to analyse the comparative efficacy of a novel Antibiotic Adjuvant Entity (AAE), a combination of ceftriaxone + sulbactam + disodium edetate and meropenem in combination with colistin, for the management of Multi Drug Resistant (MDR) nosocomial Gram-negative bacterial infections...
December 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28207311/prevalence-of-salmonella-in-cashews-hazelnuts-macadamia-nuts-pecans-pine-nuts-and-walnuts-in-the-united-states
#12
Guodong Zhang, Lijun Hu, David Melka, Hua Wang, Anna Laasri, Eric W Brown, Errol Strain, Marc Allard, Vincent K Bunning, Steven M Musser, Rhoma Johnson, Sofia M Santillana Farakos, Virginia N Scott, Régis Pouillot, Jane M Van Doren, Thomas S Hammack
Nuts have been identified as a vector for salmonellosis. The objective of this project was to estimate the prevalence and contamination level of Salmonella in raw tree nuts (cashews, pecans, hazelnuts, macadamia nuts, pine nuts, and walnuts) at retail markets in the United States. A total of 3,656 samples of six types of tree nuts were collected from different types of retail stores and markets nationwide between October 2014 and October 2015. These samples were analyzed using a modified version of the Salmonella culture method from the U...
February 16, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28202329/efficacy-of-sulbactam-for-the-treatment-of-acinetobacter-baumannii-complex-infection-a-systematic-review-and-meta-analysis
#13
Haojun Chen, Qianqian Liu, Zhanguo Chen, Congrong Li
Meta-analyses that ignore the full programme of clinical trials may lead to a misleading interpretation. We did a comprehensive meta-analysis to explore the efficacy of sulbactam for the treatment of Acinetobacter baumannii complex infection. We searched electronic databases, including Pubmed and Embase up to April 24, 2016, to identify relevant published trials. Clinical trial registries were likewise searched to identify completed unpublished studies. Primary outcomes of interest were the clinical and microbiological efficacy and in-hospital mortality...
February 13, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28199150/development-and-validation-of-a-cultural-method-for-the-detection-and-isolation-of-salmonella-in-cloves
#14
Guodong Zhang, Laila Ali, Vikas Gill, Aparna Tatavarthy, Xiaohong Deng, Lijun Hu, Eric W Brown, Thomas S Hammack
Detection of Salmonella in some spices, such as cloves, remains a challenge due to their inherent antimicrobial properties. The purpose of this study was to develop an effective detection method for Salmonella from spices using cloves as a model. Two clove varieties, Ceylon and Madagascar, were used in the study. Cloves were inoculated with Salmonella enterica subsp. enterica serotypes Montevideo, Typhimurium, or Weltevreden at about 1, 3, or 6 log CFU/25 g. Two test portion sizes, 10 and 25 g, were compared...
February 15, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28193742/delays-and-pathways-to-final-tuberculosis-diagnosis-in-patients-from-a-referral-hospital-in-urban-china
#15
Leonardo Martinez, Lin Xu, Cheng Chen, Juliet N Sekandi, Yongzhong Zhu, Changsheng Zhang, Christopher C Whalen, Limei Zhu
China is among the countries with the largest epidemic of drug susceptible and resistant tuberculosis globally. We investigated the locations tuberculosis patients visited before being diagnosed, total diagnostic delay, and risk factors associated with total delay from a large tuberculosis referral hospital in Nanjing, China. We conducted a retrospective cohort study among tuberculosis patients who initiated anti-tuberculosis treatment within 3 months prior to the study date. Patient information regarding time and locations visited while seeking care for tuberculosis-related symptoms was collected through face-to-face interviews...
February 13, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28192236/diagnosis-of-primary-antibody-and-complement-immunodeficiencies-in-young-adults-after-a-first-invasive-bacterial-infection
#16
Sébastien Sanges, Frédéric Wallet, Nicolas Blondiaux, Didier Theis, Isabelle Verin, Anne Vachée, Rodrigue Dessein, Karine Faure, Nathalie Viget, Eric Senneville, Olivier Leroy, Fleur Maury, Nicolas Just, Julien Poissy, Daniel Mathieu, Anne Prévotat, Cécile Chenivesse, Arnaud Scherpereel, Grégoire Smith, Benjamin Lopez, Jérémie Rosain, Véronique Fremeaux-Bacchi, Eric Hachulla, Pierre-Yves Hatron, Mathilde Bahuaud, Frédéric Batteux, David Launay, Myriam Labalette, Guillaume Lefèvre
OBJECTIVES: Screening for primary immunodeficiencies (PIDs) in adults is recommended after two severe bacterial infections. We aimed to evaluate if screening should be performed after the first invasive infection in young adults. METHODS: Eligible patients were retrospectively identified using hospital discharge and bacteriology databases in 3 centers during a 3-year period. Eighteen to forty year-old patients were included if they had experienced an invasive infection with encapsulated bacteria commonly encountered in PIDs (Streptococcus pneumoniae (SP), Neisseria meningitidis (NM), Neisseria gonorrhoeae (NG), Haemophilus influenzae (HI) or group A Streptococcus (GAS))...
February 10, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28182705/low-implementation-of-xpert-mtb-rif-among-hiv-tb-co-infected-adults-in-the-international-epidemiologic-databases-to-evaluate-aids-iedea-program
#17
Kate Clouse, Meridith Blevins, Mary Lou Lindegren, Marcel Yotebieng, Dung Thi Nguyen, Alfred Omondi, Denna Michael, Djimon Marcel Zannou, Gabriela Carriquiry, April Pettit
OBJECTIVE: Xpert MTB/RIF is recommended by the World Health Organization (WHO) as the initial tuberculosis (TB) diagnostic test in individuals suspected of HIV-associated TB. We sought to evaluate field implementation of Xpert among a cohort of HIV/TB co-infected individuals, including availability, utilization and outcomes. DESIGN: Observational cohort study (patient-level data) and cross-sectional study (site-level Xpert availability data). METHODS: Data were collected at 30 participating International epidemiologic Databases to Evaluate AIDS (IeDEA) sites in 18 countries from January 2012-January 2016...
2017: PloS One
https://www.readbyqxmd.com/read/28182358/aetiology-and-significance-of-hospital-acquired-infections-in-mexico
#18
REVIEW
Nayelli M Alonso-Aguilar, Sara R Juarez-Enriquez, Graciela Castro-Escarpulli, Gildardo Rivera, Virgilio Bocanegra-Garcia, Xianwu Guo, Julieta Luna-Herrera, M G Aguilera-Arreola
Hospital-acquired infections (HAIs) are infections that develop in the hospital environment and can be acquired by a patient or hospital staff. They are complications that combine diverse risk factors that make an individual susceptible and are frequently caused by endogenous and exogenous bacterial agents. The most commonly studied etiological agents are bacteria and fungi, with the former representing the most common etiological agents reported to the Hospital Epidemiological Surveillance Network (RHOVE) between 2007 and 2012...
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28178984/bacteriological-water-quality-in-school-s-drinking-fountains-and-detection-antibiotic-resistance-genes
#19
Denize Gomes Freitas, Rassan Dyego Romão Silva, Luis Artur Mendes Bataus, Mônica Santiago Barbosa, Carla Afonso da Silva Bitencourt Braga, Lilian Carla Carneiro
The fecal coliform can contaminate water of human consumption causing problems to public health. Many of these microorganisms may contain plasmid and transfer them to other bacteria. This genetic material may confer selective advantages, among them resistance to antibiotics. The objectives of this study were to analyze the presence of fecal coliforms in water and at drinker surface, to identify the existence of plasmid, conducting studies of resistance to antibiotics, plasmid stability and capacity of bacterial conjugation...
February 8, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28178199/combined-use-of-delamanid-and-bedaquiline-to-treat-multidrug-resistant-and-extensively-drug-resistant-tuberculosis-a-systematic-review
#20
REVIEW
Giovanni Battista Migliori, Emanuele Pontali, Giovanni Sotgiu, Rosella Centis, Lia D'Ambrosio, Simon Tiberi, Marina Tadolini, Susanna Esposito
The new drugs delamanid and bedaquiline are increasingly being used to treat multidrug-resistant (MDR-) and extensively drug-resistant tuberculosis (XDR-TB). The World Health Organization, based on lack of evidence, recommends their use under specific conditions and not in combination. No systematic review has yet evaluated the efficacy, safety, and tolerability of delamanid and bedaquiline used in combination. A search of peer-reviewed, scientific evidence was carried out, aimed at evaluating the efficacy/effectiveness, safety, and tolerability of delamanid and bedaquiline-containing regimens in individuals with pulmonary/extrapulmonary disease, which were bacteriologically confirmed as M/XDR-TB...
February 7, 2017: International Journal of Molecular Sciences
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