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https://www.readbyqxmd.com/read/28319799/occurrence-of-hepatitis-a-and-e-and-norovirus-gi-and-gii-in-ready-to-eat-vegetables-in-italy
#1
V Terio, M Bottaro, E Pavoni, M N Losio, A Serraino, F Giacometti, V Martella, A Mottola, A Di Pinto, G Tantillo
Fresh vegetables and their ready-to-eat (RTE) salads have become increasingly recognized as potential vehicles for foodborne diseases. The EU Reg. 1441/2007 establishes microbiological criteria for bacterial pathogens for products placed on the market during their shelf-life (i.e. Salmonella spp., Listeria monocytogenes) for pre-cut fruits and vegetables (RTE) whilst it does not address the problem of contamination by enteric viruses. In this study we investigated the contamination by hepatitis A virus (HAV), hepatitis E virus (HEV) and norovirus (NoV) in 911 ready-to-eat vegetable samples taken from products at retail in Apulia and in Lombardia...
March 14, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28317806/mycobacterium-lepraemurium-uses-tlr-6-and-mr-but-not-lipid-rafts-or-dc-sign-to-gain-access-into-mouse-macrophages
#2
Mayra Silva-Miranda, Patricia Arce-Paredes, Oscar Rojas-Espinosa
OBJECTIVE/BACKGROUND: Mycobacterium lepraemurium (MLM), the etiologic agent of murine leprosy, is an intracellular parasite of macrophages; the mechanism used by this bacterium to enter macrophages is not known. The fate of the MLM phagosome inside macrophages is also unknown. This study was conducted to investigate how MLM enters macrophages and to define the maturation process of MLM phagosome inside macrophages. MATERIALS AND METHODS: Peritoneal macrophages were incubated in the presence of mannan-bovine serum albumin (BSA), and antibodies to known macrophage receptors, including, anti-FcγRIII/RII (anti-CD16/32), anti-CD35 (anti-CR1), anti-TLR2, anti-TLR4, anti-TLR6, anti-CD14, and anti-dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN)...
January 2017: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28315659/lessons-learned-with-molecular-methods-targeting-the-bcsp-31-membrane-protein-for-diagnosis-of-human-brucellosis
#3
REVIEW
Rocio Sanjuan-Jimenez, Juan D Colmenero, Pilar Morata
Brucellosis remains an emerging and re-emerging zoonosis worldwide causing high human morbidity. It usually affects persons who are permanently exposed to fastidious microorganisms of the Brucella genus and has a nonspecific clinical picture. Thus, diagnosis of brucellosis can sometimes be difficult. Molecular techniques have recently been found very useful in the diagnosis of brucellosis together with its common and very diverse focal complications. We herein review all the lessons learned by our group concerning the molecular diagnosis of human brucellosis over the last twenty years...
March 15, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28315144/monitoring-antimicrobial-resistance-amr-using-cusum-control-charts
#4
L Righi, R Amarsy, M-Q Picat, M Thuillier, E Cambau, L Raskine, S Chevret, R Flicoteaux
We evaluated the use of the Cumulative Summation (CUSUM) control chart methodology for detection of an excessive increase in antimicrobial-resistant (AMR) bacteria acquisition. We used administrative, clinical and bacteriological data from all 157,570 patients hospitalized for at least 48 h from January 1, 2010 to December 31, 2015 in a 654-bed university teaching hospital in Paris, France. Monthly computed CUSUM were evaluated for the detection of out-of-control situations, defined as incidence rates of acquired AMR bacterial colonization exceeding acceptable thresholds at the hospital and ward levels (based on six selected wards) for AMR bacteria overall and Extended-spectrum beta-lactamases Enterobacteriaceae (ESBL-E) and Methicillin-resistant Staphylococcus aureus (MRSA), specifically...
March 17, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28314997/clinical-and-microbiological-outcomes-in-haematogenous-spondylodiscitis-treated-conservatively
#5
Tiziana Ascione, Giovanni Balato, Sigismondo Luca Di Donato, Pasquale Pagliano, Francesco Granata, Gianluca Colella, Carlo Ruosi
INTRODUCTION: Spondylodiscitis refers to infections of the intervertebral disc and the adjacent vertebral body. Although it is still considered a rare condition, its rate is projected to increase. Mortality rate is considered to be low, but an estimated one third of the survivors experience residual disabilities. Literature shows that uncomplicated spondylodiscitis can be adequately treated by early antibiotic therapy and immobilization. The aim of the study is to evaluate the outcome of conservative treatment in patients with haematogenous spondylodiscitis...
March 17, 2017: European Spine Journal
https://www.readbyqxmd.com/read/28314783/umbilical-infections-in-foals-microbiological-investigation-and-management
#6
E Rampacci, F Passamonti, M Bottinelli, V Stefanetti, M Cercone, S Nannarone, R Gialletti, F Beccati, M Coletti, M Pepe
This study aims to investigate the bacteria involved in equine omphalitis and their susceptibility to antimicrobial drugs, and consequently to provide guidelines concerning the most suitable treatment protocol in accordance with the clinical, ultrasound and laboratory findings. Forty foals aged between one and 30 days were evaluated in the course of this investigation. An ultrasound examination of all umbilical remnants was carried out carefully in all foals; umbilical swabs were collected for bacteriological examination, and blood samples were collected for blood culture from 19 foals with fever and abnormal blood values...
March 17, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28303655/cholangiohepatitis-in-dairy-cattle-13-cases
#7
D E Gomez, E Doré, D Francoz, A Desrochers, H Pierre, G Fecteau
BACKGROUND: The signalment, clinicopathologic, bacteriological, histopathological, ultrasonographic characteristics, and the treatment and outcomes of adult cattle with cholangiohepatitis are poorly defined. ANIMALS: Thirteen Holstein cows with cholangiohepatitis. METHODS: Retrospective study of medical records of cattle admitted to the CHUV and the AVC between 1992 and 2012 and 2000 and 2012, respectively, for cattle older than 3 months of age with a histopathological diagnosis of cholangiohepatitis...
March 16, 2017: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/28302070/prevalence-and-incidence-of-smear-positive-pulmonary-tuberculosis-in-the-hetosa-district-of-arsi-zone-oromia-regional-state-of-central-ethiopia
#8
ShalloDaba Hamusse, Meaza Demissie, Dejene Teshome, Mohammed Suaudi Hassen, Bernt Lindtjørn
BACKGROUND: The real burden of smear-positive (PTB+) and bacteriologically confirmed tuberculosis (BCTB) in Ethiopia is not known. Thus, the aim of this community-based study was to measure the prevalence and incidence of tuberculosis in the Hetosa District of Oromia Region, Ethiopia. METHODS: First, a population-based cross-sectional survey was conducted on a total of 33,073 individuals aged ≥ 15 years to determine the prevalence of PTB+ and BCTB cases. Then, in order to determine the incidence, a prospective follow-up was carried out on 32,800 individuals found to be either free from symptoms suggestive of TB (SSTB) during the baseline survey or had symptoms suggestive of TB but yielded negative bacteriological examination results...
March 16, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28301236/coinfection-of-avibacterium-paragallinarum-and-gallibacterium-anatis-in-specific-pathogen-free-chickens-complicates-clinical-signs-of-infectious-coryza-which-can-be-prevented-by-vaccination
#9
Surya Paudel, Michael Hess, Claudia Hess
Avibacterium paragallinarum and Gallibacterium anatis are recognized bacterial pathogens both infecting the respiratory tract of chickens. The present study investigated outcomes of their coinfection by elucidating clinical signs, pathologic lesions, and bacteriologic findings. Additionally, the efficacy of a commercially available vaccine to prevent diseases caused by A. paragallinarum and G. anatis was evaluated. Birds inoculated with G. anatis alone did not present any clinical signs and gross pathologic lesions in the respiratory tract...
March 2017: Avian Diseases
https://www.readbyqxmd.com/read/28301230/three-cases-of-escherichia-coli-meningitis-in-chicks-imported-to-japan
#10
Kazufumi Kasuya, Natsumi Shimokubo, Chieko Kosuge, Kou Takayama, Eiji Yoshida, Hiroyuki Osaka
Three outbreaks of colibacillosis have occurred in chicks during the quarantine period after importation to Japan. All three were derived from three different countries without epidemiologic relevance. Some birds from each infected flock were examined pathologically and bacteriologically. The characteristic histologic finding common to all three cases was severe bacterial meningitis in the central nervous system. Pericarditis, perihepatitis, and omphalitis with bacterial colonies were also observed. The bacterial colonies observed histologically were immunohistochemically positive for Escherichia coli antigens...
March 2017: Avian Diseases
https://www.readbyqxmd.com/read/28296252/research-on-handwashing-techniques-of-peritoneal-dialysis-patients-from-yiwu-southeast-china
#11
Yuanjun Ye, Xiaohui Zhang, Yansu Liu, Hongqing Lou, Zhangfei Shou
The aim of the present study was to evaluate handwashing technique, bacteriology, and factors influencing handwashing technique of 86 stable chronic peritoneal dialysis (PD) patients from Yiwu City in Southeast China. Based on the "Hygienic standard for disinfection in the hospital", we also performed sampling for bacteriology from PD operators after they washed their hands. We compared their clinical features including the pathogenic bacteria of their previous peritonitis episodes and their handwashing evaluation results according to their bacteriologic sampling results...
March 10, 2017: Therapeutic Apheresis and Dialysis
https://www.readbyqxmd.com/read/28291538/-pyoderma-gangrenosum-associated-with-anti-proteinase-3%C3%A2-antineutrophil-cytoplasmic-antibodies-pr3-anca-induced-by-propylthiouracil
#12
A Coster, J-L Dargent, N de Visscher, P Levecque, P-P Roquet-Gravy
BACKGROUND: Synthetic antithyroid drugs are often used in the treatment of hyperthyroidism, regardless of aetiology. They may cause various side effects, including the development of anti-neutrophil cytoplasmic antibodies (ANCA), ANCA-associated vasculitis, and neutrophilic dermatoses. Propylthiouracil (PTU) is the antithyroid drug most frequently implicated in ANCA-associated diseases specifically involving anti-myeloperoxidase ANCA (MPO-ANCA). To our knowledge, there are no clinical reports describing the association of pyoderma gangrenosum (PG) and anti-proteinase3-ANCA (PR3-ANCA) induced by PTU, with ANCA levels decreasing after antithyroid drug withdrawal...
March 10, 2017: Annales de Dermatologie et de Vénéréologie
https://www.readbyqxmd.com/read/28290692/amphiphilic-indole-derivatives-as-antimycobacterial-agents-structure-activity-relationships-and-membrane-targeting-properties
#13
Tianming Yang, Wilfried Moreira, Samuel Agyei Nyantakyi, Huan Chen, Dinah Binte Aziz, Mei-Lin Go, Thomas Dick
Antibacterials that disrupt cell membrane function have the potential to eradicate 'persister' organisms and delay the emergence of resistance. Here we report the antimycobacterial activities of 4-fluoro and 6-methoxyindoles bearing a cationic amphiphilic motif represented by a lipophilic n-octyl side chain at position 1 and a positively charged azepanyl or 1,4-dioxa-8-azaspiro[4.5]decane moiety at position 3. These analogs exhibited balanced profiles of potency (Mycobacterium bovis BCG, M tuberculosis H37Rv), selective activity, solubility and metabolic stability...
March 14, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28286386/brain-abscess-potentially-resulting-from-odontogenic-focus-report-of-three-cases-and-a-literature-review
#14
Masaya Akashi, Kazuhiro Tanaka, Junya Kusumoto, Shungo Furudoi, Kohkichi Hosoda, Takahide Komori
INTRODUCTION: Odontogenic foci can rarely cause intracranial infection. Hematogenous spread is considered to be the most important pathophysiological mechanism of intracranial infection of odontogenic origin. To investigate the oral origin of intracranial infections, oral surgeons should understand the underlying mechanisms by which oral bacteria spread to the central nervous system. However, there have been very few reports of intracranial infection resulting from odontogenic infection...
March 2017: Journal of Maxillofacial and Oral Surgery
https://www.readbyqxmd.com/read/28285498/profiling-total-viable-bacteria-in-a-hemodialysis-water-treatment-system
#15
Lihua Chen, Xuan Zhu, Menglu Zhang, Yuxin Wang, Tianyu Lv, Shenghua Zhang, Xin Yu
The culture-dependent methods such as heterotrophic plate counting (HPC) are usually applied to evaluate the bacteriological quality of the hemodialysis water. However, these methods cannot detect the uncultured or viable but non-culturable (VBNC) bacteria both of which may be quantitatively predominant throughout the hemodialysis water treatment system. Therefore, PMA-qPCR associated with HPC was used together to profile the distribution of the total viable bacteria in such a system. Moreover, high-throughput sequencing of 16s rRNA gene amplicons was utilized to analyze the microbial community structure and diversity...
March 13, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28284321/matrix-metalloproteinases-and-airway-remodeling-and-function-in-primary-ciliary-dyskinesia
#16
Massimo Pifferi, Andrew Bush, Davide Caramella, Maria Rita Metelli, Maria Di Cicco, Martina Piras, Giulia Gherarducci, Carlo Capristo, Fabrizio Maggi, Diego Peroni, Attilio L Boner
BACKGROUND: The balance between matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) is important in the regulation of airway damage. OBJECTIVE: To evaluate whether they are important in the pathophysiology of primary and secondary ciliary dyskinesia (PCD, SCD). METHODS: We measured sputum bacteriology, lung CT changes, MMPs, TIMPs and lung function in 86 patients (51 PCD, 35 SCD) in a cross-sectional study; the 10 controls studied did not have HRCT or sputum cultures...
March 2017: Respiratory Medicine
https://www.readbyqxmd.com/read/28284260/tuberculosis-in-hiv-infected-south-african-children-with-complicated-severe-acute-malnutrition
#17
H Adler, M Archary, P Mahabeer, P LaRussa, R A Bobat
SETTING: Academic tertiary referral hospital in Durban, South Africa. OBJECTIVE: To describe the incidence and diagnostic challenges of tuberculosis (TB) in human immunodeficiency virus (HIV) infected children with severe acute malnutrition (SAM). DESIGN: Post-hoc analysis of a randomised controlled trial that enrolled antiretroviral therapy naïve, HIV-infected children with SAM. Trial records and hospital laboratory results were explored for clinical diagnoses and bacteriologically confirmed cases of TB...
April 1, 2017: International Journal of Tuberculosis and Lung Disease
https://www.readbyqxmd.com/read/28280293/cervicovaginal-bacteriology-and-antibiotic-sensitivity-patterns-among-women-with-premature-rupture-of-membranes-in-mulago-hospital-kampala-uganda-a-cross-sectional-study
#18
Milton W Musaba, Mike N Kagawa, Charles Kiggundu, Paul Kiondo, Julius Wandabwa
Background. A 2013 Cochrane review concluded that the choice of antibiotics for prophylaxis in PROM is not clear. In Uganda, a combination of oral erythromycin and amoxicillin is the 1st line for prophylaxis against ascending infection. Our aim was to establish the current cervicovaginal bacteriology and antibiotic sensitivity patterns. Methods. Liquor was collected aseptically from the endocervical canal and pool in the posterior fornix of the vagina using a pipette. Aerobic cultures were performed on blood, chocolate, and MacConkey agar and incubated at 35-37°C for 24-48 hrs...
2017: Infectious Diseases in Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28278183/systemic-thioridazine-in-combination-with-dicloxacillin-against-early-aortic-graft-infections-caused-by-staphylococcus-aureus-in-a-porcine-model-in-vivo-results-do-not-reproduce-the-in-vitro-synergistic-activity
#19
Michael Stenger, Carsten Behr-Rasmussen, Kasper Klein, Rasmus B Grønnemose, Thomas Emil Andersen, Janne K Klitgaard, Hans Jørn Kolmos, Jes S Lindholt
INTRODUCTION: Conservative treatment solutions against aortic prosthetic vascular graft infection (APVGI) for inoperable patients are limited. The combination of antibiotics with antibacterial helper compounds, such as the neuroleptic drug thioridazine (TDZ), should be explored. AIM: To investigate the efficacy of conservative systemic treatment with dicloxacillin (DCX) in combination with TDZ (DCX+TDZ), compared to DCX alone, against early APVGI caused by methicillin-sensitive Staphylococcus aureus (MSSA) in a porcine model...
2017: PloS One
https://www.readbyqxmd.com/read/28274807/virulence-genes-and-genetic-relationship-of-l-monocytogenes-isolated-from-human-and-food-sources-in-brazil
#20
Rosana Macedo de Almeida, André Victor Barbosa, Rodrigo de Castro Lisbôa, André Felipe das Mercês Santos, Ernesto Hofer, Deyse Christina Vallim, Cristina Barroso Hofer
The herein presented assay provided a bacteriological and molecular characterization of 100 samples of L. monocytogenes isolated from human (43) and food (57) sources, from several regions of Brazil, and collected between 1975 and 2013. Antigenic characterization defined 49% of serotype 4b samples, followed by 28% of serotype 1/2b, 14% of serotype 1/2c, 8% of serotype 1/2a, and 1% of serotype 3b. Both type of samples from human and food origin express the same serotype distribution. Multiplex PCR analysis showed 13 strains of type 4b with the amplification profile 4b-VI (Variant I)...
March 5, 2017: Brazilian Journal of Infectious Diseases
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