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https://www.readbyqxmd.com/read/28449183/noninferiority-trial-investigating-the-efficacy-of-a-nonantibiotic-intramammary-therapy-in-the-treatment-of-mild-to-moderate-clinical-mastitis-in-dairy-cows-with-longer-lasting-udder-diseases
#1
M Ziesch, N Wente, Y Zhang, W Zaremba, S Engl, V Krömker
A nonblinded, positively controlled, noninferiority trial was conducted to evaluate the efficacy of an alternative, nonantibiotic therapy with Masti Veyxym(®) to reduce ineffective antibiotic usage in the treatment of nonsevere clinical mastitis (CM) in cows with longer lasting udder diseases. The solely intramammary treatment with Masti Veyxym(®) (three applications, 12 hr apart) and the combined treatment with Masti Veyxym(®) and antibiotics as usual on the farm according to label of the respective product were compared with the reference treatment of solely antibiotic therapy...
April 27, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28447965/perspectives-of-using-of-aseptic-drains-for-abdominal-drainage
#2
Merab Kiladze, Paata Tutberidze, Maka Gogoladze, David Tugushi, Ramaz Katsarava, Tsismari Gatenadze
AIM: Aim of the study was to evaluate the effectiveness of using different types of drain tubes to prevent and reduce the drain-associated infection rate of abdominal drainage procedures. MATERIALS AND METHODS: 80 cases of used so called "standard", "coladerm" and "chlorhexidine" drain tubes for abdominal drainage were analysed. "Standard" drain tubes were used 35 times and "coladerm" and "chlorhexidine" tubes - 20 and 25 times respectively. There were adopted in different elective and emergency so called "clean", "potentially contaminated" and "contaminated" abdominal surgical procedures...
2017: Annali Italiani di Chirurgia
https://www.readbyqxmd.com/read/28443226/a-novel-pcr-assay-for-detecting-brucella-abortus-and-brucella-melitensis
#3
Saeed Alamian, Majid Esmaelizad, Taghi Zahraei, Afshar Etemadi, Mohsen Mohammadi, Davoud Afshar, Soheila Ghaderi
OBJECTIVES: Brucellosis is a major zoonotic disease that poses a significant public health threat worldwide. The classical bacteriological detection process used to identify Brucella spp. is difficult and time-consuming. This study aimed to develop a novel molecular assay for detecting brucellosis. METHODS: All complete sequences of chromosome 1 with 2.1-Mbp lengths were compared among all available Brucella sequences. A unique repeat sequence (URS) locus on chromosome 1 could differentiate Brucella abortus from Brucella melitensis...
February 2017: Osong Public Health and Research Perspectives
https://www.readbyqxmd.com/read/28442112/experimental-infection-with-yersinia-pseudotuberculosis-in-european-brown-hare-lepus-europaeus-pallas
#4
Filippo Fratini, Ranieri Verin, Valentina V Ebani, Cecilia Ambrogi, Fabrizio Bertelloni, Barbara Turchi, Alessandro Poli, Domenico Cerri
OBJECTIVE: To investigate clinicopathological, bacteriological and pathological aspects of an experimental infection with Yersinia pseudotuberculosis (Y. pseudotuberculosis) in hares to verify the efficacy of serology for the in vivo diagnosis. Moreover, the pathogenicity of two Y. pseudotuberculosis strains was investigated in order to detect potential differences. METHODS: Twelve European brown hares (Lepus europaeus, Pallas) were experimentally infected per os and via conjunctival mucosae with Y...
March 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28441365/efficacy-and-safety-of-3-nasal-packing-materials-used-after-functional-endoscopic-sinus-surgery-for-chronic-rhinosinusitis-a-comparative-study-in-china
#5
Xi-Ling Zheng, Yu-Xiang Zhao, Min Xu
BACKGROUND This study aimed to investigate the efficacy and safety of 3 nasal packing products (silicone tube, Beschitin-F, and Aquacel1-Ag) after functional endoscopic sinus surgery (FESS) for chronic rhinosinusitis (CRS) patients in China. MATERIAL AND METHODS Sixty-six CRS patients undergoing FESS surgery were randomly divided into 3 groups (22 patients in each group): the silicone tube group, the Beschitin-F group, and the Aquacel1-Ag group. Postoperative headache, nasal pain, nasal bleeding, and swelling of the nasal mucosa were observed at 1 month after FESS surgery...
April 25, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28439041/cues-from-the-membrane-bacterial-glycerophospholipids
#6
Zachary D Dalebroux
In this issue of Journal of Bacteriology, Rowlett et al. unveil new Escherichia coli circuitry linking membrane glycerophospholipid (GPL) homeostasis to bacterial stress response and adaptation mechanisms. Glycerophospholipids comprise critical components of the dual-membrane envelope of Gram-negative bacteria and participate in many processes. The new evidence suggests that in some instances distinct E. coli GPL molecules function for distinct biochemistry, and bacteria sense perturbations in membrane GPL concentrations to coordinate survival strategies...
April 24, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28438936/demonstrating-the-protective-efficacy-of-the-novel-fluoroquinolone-finafloxacin-against-an-inhalational-exposure-to-burkholderia-pseudomallei
#7
Kay B Barnes, Karleigh A Hamblin, Mark I Richards, Thomas R Laws, Andreas Vente, Helen S Atkins, Sarah V Harding
Burkholderia pseudomallei is the causative agent of melioidosis, a serious disease endemic in South East Asia and Northern Australia. Antibiotic treatment is lengthy and relapse often occurs. Finafloxacin is a novel fluoroquinolone with increased antibacterial activity in acidic conditions in contrast to other fluoroquinolones which demonstrate reduced activity at lower pH. Therefore finafloxacin may have improved efficacy against B. pseudomallei, which can survive within host cells where the local pH is acidic...
April 24, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28438139/digital-cxr-with-computer-aided-diagnosis-versus-symptom-screen-to-define-presumptive-tuberculosis-among-household-contacts-and-impact-on-tuberculosis-diagnosis
#8
Monde Muyoyeta, Nkatya Chanda Kasese, Deborah Milimo, Isaac Mushanga, Mapopa Ndhlovu, Nathan Kapata, Maureen Moyo-Chilufya, Helen Ayles
BACKGROUND: Household (HH) contact tracing is a strategy that targets high risk groups for TB. Symptom based screening is the standard used to identify HH contacts at risk for TB during HH contact tracing for TB. However, this strategy may be limited due to poor performance in predicting TB. The objective of this study was to compare CXR with Computer Aided Diagnosis (CAD) against symptom screen for defining presumptive TB and how TB detection changes with each method. METHODS: Household contacts of consecutive index bacteriologically confirmed TB cases were visited by study teams and given TB/HIV education to raise awareness of the risk of TB following close contact with a TB patient...
April 24, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28435894/oral-bacterial-acid-base-metabolism-in-caries-screening-a-proof-of-concept-study
#9
E Morou-Bermudez, M A Loza-Herrero, V Garcia-Rivas, E Suarez-Perez, R J Billings
The objective of this cross-sectional study was to clinically validate an array of biochemical tests for oral acid/alkali generation as caries screening instruments. 185 adult subjects (mean 33.6±10.6 years) were examined clinically for dental caries using the ICDAS criteria. Bitewing radiographs were used to confirm interproximal surfaces of posterior teeth. For the purposes of this study, subjects were classified as "caries-active" if they had at least one untreated caries lesion with ICDAS 4 or higher. Pooled supragingival plaque and unstimulated saliva samples were collected and assayed for pH changes from sucrose and urea metabolism using colorimetric tests...
April 2017: JDR Clinical and Translational Research
https://www.readbyqxmd.com/read/28431785/multicausal-etiology-of-the-enteric-syndrome-in-rabbits-from-mexico
#10
Virginia G García-Rubio, Linda G Bautista-Gómez, José S Martínez-Castañeda, Camilo Romero-Núñez
Enteropathies in rabbits are difficult to diagnose; their etiology involves pathogens that act synergistically, causing damage to the intestine. The aim of the present study was isolate enteric pathogens from rabbits in Mexico. Using parasitological, bacteriological and molecular analyses, we screened 58 samples of the intestinal content of rabbits having a clinical history of enteric disease from the southeastern part of the State of Mexico. Out of the 58 samples analyzed, a total of 86 identifications were made, Eimeria spp...
April 18, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/28429162/involvement-of-methylated-hbha-expressed-from-mycobacterium-smegmatis-in-an-ifn-%C3%AE-release-assay-to-aid-discrimination-between-latent-infection-and-active-tuberculosis-in-bcg-vaccinated-populations
#11
H-L Wen, C-L Li, G Li, Y-H Lu, H-C Li, T Li, H-M Zhao, K Wu, D B Lowrie, J-X Lv, S-H Lu, X-Y Fan
IFN-γ release assays (IGRAs) based on region of difference 1 (RD1) antigens have improved diagnosis of Mycobacterium tuberculosis (M. tb) infection. However, IGRAs with these antigens cannot discriminate between active tuberculosis (ATB) and latent tuberculosis infection (LTBI). M. tb heparin-binding-hemagglutinin (HBHA) induces relatively high IFN-γ responses in LTBI individuals and low responses in ATB patients, but purification of the native methylated HBHA from cultures of M. tb for immunological tests is complex and time-consuming...
April 20, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28428678/productive-cough-in-children-and-adolescents-view-from-primary-health-care-system
#12
REVIEW
Edin Begic, Zijo Begic, Amra Dobraca, Edo Hasanbegovic
Cough is one of the most common symptoms that doctor faces in working with pediatric population, and according to some characteristics of cough, doctors can often conclude localization, and sometimes even the nature of the disease that causes it. Cough is not only the physiological defense reaction, but a symptom of a disease. According to duration it can be acute, chronic and recidivist, recurrent and persistent, strong or discreet, caused by changes in body position and changes in outside temperature. Pathoanatomically it is divided into lobar, lobular, alveolar and interstitial, pathogenetically to bronchogenic and hematogenous, as well as in immuno competent and immunocompromised, and clinically on the local and inpatient (72 hours after hospital admission)...
February 2017: Medical Archives
https://www.readbyqxmd.com/read/28428133/epidemiological-study-of-common-diseases-and-their-risk-factors-in-camels-in-south-punjab-pakistan
#13
Aneela Zameer Durrani, Zubair Bashir, Imran Rasheed, Noor-Ul-Ain Sarwar
Bacteriological study of mastitis along with common blood protozoan diseases were studied in dromedary camels in Cholistan, Dera Ismail Khan and Rahim Yar Khan districts in South Punjab, Pakistan. For this purpose 300 camels were sampled randomly at different common grazing and watering point. For study of blood parasites clinically suspected and apparently healthy camels, 150 each, were sampled. An overall prevalence of 15%and 5% was recorded for trypanosomiasis and Anaplasmosis respectively. Trypanosoma evansi was identified with 280 bp product on polymerase chain reaction test...
April 17, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28425146/a-deep-nasopharyngeal-swab-versus-nonendoscopic-bronchoalveolar-lavage-for-isolation-of-bacterial-pathogens-from-preweaned-calves-with-respiratory-disease
#14
L Van Driessche, B R Valgaeren, L Gille, F Boyen, R Ducatelle, F Haesebrouck, P Deprez, B Pardon
BACKGROUND: Nonendoscopic bronchoalveolar lavage (BAL) is a practical alternative for a deep nasopharyngeal swab (DNS) to sample the airways of a large number of calves in a short period of time. The extent of commensal overgrowth and agreement of BAL with DNS culture results in preweaned calves are unknown. OBJECTIVES: To compare commensal overgrowth and bacterial culture results between DNS and BAL samples. ANIMALS: A total of 183 preweaned calves (144 with bovine respiratory disease and 39 healthy animals)...
April 19, 2017: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/28424675/safety-protective-immunity-and-diva-capability-of-a-rough-mutant-salmonella-pullorum-vaccine-candidate-in-broilers
#15
Rongxian Guo, Yang Jiao, Zhuoyang Li, Shanshan Zhu, Xiao Fei, Shizhong Geng, Zhiming Pan, Xiang Chen, Qiuchun Li, Xinan Jiao
Salmonella enterica subsp. enterica serovar Gallinarum biovar Pullorum (Salmonella Pullorum) is highly adapted to chickens causing an acute systemic disease that results in high mortality. Vaccination represents one approach for promoting animal health, food safety and reducing environmental persistence in Salmonella control. An important consideration is that Salmonella vaccination in poultry should not interfere with the salmonellosis monitoring program. This is the basis of the DIVA (Differentiation of Infected and Vaccinated Animals) program...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28419315/clinical-parameters-routine-inflammatory-markers-and-lta4h-genotype-as-predictors-of-mortality-among-608-patients-with-tuberculous-meningitis-in-indonesia
#16
Arjan van Laarhoven, Sofiati Dian, Carolien Ruesen, Ela Hayati, Michelle S M A Damen, Jessi Annisa, Lidya Chaidir, Rovina Ruslami, Tri Hanggono Achmad, Mihai G Netea, Bachti Alisjahbana, Ahmad Rizal Ganiem, Reinout van Crevel
Background.: Damaging inflammation is thought to contribute to the high morbidity and mortality of tuberculous meningitis (TBM), but the link between inflammation and outcome remains unclear. Methods.: We performed prospective clinical and routine laboratory analyses of a cohort of adult patients with TBM in Indonesia. We also examined the LTA4H promoter polymorphism, which predicted cerebrospinal fluid (CSF) leukocyte count and survival of Vietnamese patients with TBM...
April 17, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28419236/studying-the-effect-of-administration-route-and-treatment-dose-on-the-selection-of-enrofloxacin-resistance-in-commensal-escherichia-coli-in-broilers
#17
Ilias Chantziaras, Annemieke Smet, Freddy Haesebrouck, Filip Boyen, Jeroen Dewulf
Objectives: Factors potentially contributing to fluoroquinolone resistance selection in commensal Escherichia coli strains in poultry were studied through a series of in vivo experiments. The effect of the initial prevalence of enrofloxacin resistance in the E. coli gut microbiota, effect of the bacterial fitness of the enrofloxacin-resistant strain and effect of treatment with enrofloxacin (effect of dose and effect of route of administration) were assessed. Methods: Four in vivo studies with broiler chickens were performed...
April 16, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28417060/burden-and-predictors-of-staphylococcus-aureus-and-s-pseudintermedius-infections-among-dogs-presented-at-an-academic-veterinary-hospital-in-south-africa-2007-2012
#18
Daniel N Qekwana, James Wabwire Oguttu, Fortune Sithole, Agricola Odoi
BACKGROUND: Staphylococci are commensals of the mucosal surface and skin of humans and animals, but have been implicated in infections such as otitis externa, pyoderma, urinary tract infections and post-surgical complications. Laboratory records provide useful information to help investigate these infections. Therefore, the objective of this study was to investigate the burdens of these infections and use multinomial regression to examine the associations between various Staphylococcus infections and demographic and temporal factors among dogs admitted to an academic veterinary hospital in South Africa...
2017: PeerJ
https://www.readbyqxmd.com/read/28413870/performance-evaluation-of-a-newly-developed-and-fully-automated-bacteriological-analyzer-raisus-any-for-antimicrobial-susceptibility-testing-of-fastidious-bacteria-haemophilus-influenzae-and-streptococcus-pneumoniae
#19
COMPARATIVE STUDY
Toshio Ohshima, Saori Nomiya, Yukari Yamamoto, Miki Miyazawa, Jun Ohsuga, Akifumi Hisada, Kenji Iwawaki, Satomi Asai, Hayato Miyachi
OBJECTIVE: Antimicrobial susceptibility testing for fastidious bacteria, such as Haemophilus influenzae (H. influenzae) and Streptococcus pneumoniae (S. pneumoniae) has been performed manually. We evaluated the performance of a newly developed fully automated system for rapid bacterial identification and antimicrobial susceptibility testing "RAISUS ANY" (Nissui Pharmaceutical Co., Ltd.). METHODS: We evaluated the performance of "RAISUS ANY" for measurement of minimal inhibitory concentrations (MICs) of H...
April 20, 2017: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/28412378/project-management-importance-for-diagnostic-laboratories
#20
REVIEW
Antony Croxatto, Gilbert Greub
BACKGROUND: The constraints applied on diagnostic laboratories to improve both quality and productivity together with personal shortages incite laboratory managers to constantly optimize the laboratory workflows, organization and technology. These continuous modifications of the laboratories should be conducted using efficient project and change management approaches in order to maximize the opportunities to achieve the project successfully. AIM: This review aims at presenting a general overview of project management with an emphasis on selected critical aspects...
April 12, 2017: Clinical Microbiology and Infection
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