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https://www.readbyqxmd.com/read/28925368/a-marfan-syndrome-human-induced-pluripotent-stem-cell-line-with-a-heterozygous-fbn1-c-4082g-a-mutation-ismmsi002-b-for-disease-modeling
#1
Sandra Klein, Jill L Dvornik, Akshitha R Yarrabothula, Christoph Schaniel
Fibroblasts of a 28-year-old female with Marfan syndrome (MFS) due to a heterozygous FBN1 c.4082G>A mutation were reprogrammed using the Sendai virus delivery method. The established human induced pluripotent stem cell (hiPSC) line named ISMMSi002-B expresses pluripotency markers, has a normal karyotype, carries the specific FBN1 mutation and is able to differentiate into three germ layers in vitro. ISMMSi002-B has utility in studying MFS pathogenesis, including skeletal abnormalities, cardiomyopathy, and vascular smooth muscle cell dysfunction associated with aortic aneurysm...
August 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28925367/generation-of-integration-free-induced-pluripotent-stem-cells-gzhmui001-a-by-reprogramming-peripheral-blood-mononuclear-cells-from-a-47-xxx-syndrome-patient
#2
Yuchang Chen, Zhanhui Ou, Bing Song, Yexing Xian, Shuming Ouyang, Yuhuan Xie, Yanting Xue, Xiaofang Sun
47, XXX syndrome is one of several sex-chromosomal aneuploidies, and it has an incidence of approximately 1/1000 in newborn females. Because of heterogeneity in X-inactivation, these patients may exhibit a variety of clinical symptoms. Here, we report the generation of an integration-free human induced pluripotent stem cell line (GZHMUi001-A) by using Sendai virus to reprogram peripheral blood mononuclear cells from a 47, XXX syndrome patient with premature ovarian failure. This 47, XXX iPS cell line has characteristics of pluripotent stem cells and is a useful tool for the investigation of this X chromosome aneuploid disease...
August 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28925366/establishment-of-a-human-ipsc-line-iishdoi001-a-from-a-patient-with-mcardle-disease
#3
María Del Carmen Ortuño-Costela, Nathalie Rodríguez-Mancera, Marta García-López, Francisco Zurita-Díaz, Ana Moreno-Izquierdo, Alejandro Lucía, Miguel Ángel Martín, Rafael Garesse, M Esther Gallardo
Human iPSC line IISHDOi001-A was generated from fibroblasts of a patient with McArdle disease harbouring the mutation, c.148C>T; p.Arg50Ter, in the PYGM gene. Reprogramming factors Oct3/4, Sox2, Klf4, and c-Myc were delivered using Sendai virus.
August 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28925364/generation-and-characterization-of-a-human-ipsc-line-from-a-patient-with-propionic-acidemia-due-to-defects-in-the-pcca-gene
#4
Esmeralda Alonso-Barroso, Sandra Brasil, Álvaro Briso-Montiano, Rosa Navarrete, Celia Pérez-Cerdá, Magdalena Ugarte, Belén Pérez, Lourdes R Desviat, Eva Richard
Human induced pluripotent stem cell (iPSC) line was generated from fibroblasts of a patient with propionic acidemia carrying mutations in the PCCA gene: c.1899+4_1899+7delAGTA; p.(Cys616_Val633del) and c.1430--?_1643+?del; p.(Gly477Glufs*9). Reprogramming factors OCT3/4, SOX2, KLF4 and c-MYC were delivered using a non-integrative method based on the Sendai virus. Once established, iPSCs have shown full pluripotency, differentiation capacity and genetic stability.
August 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28884293/human-parainfluenza-virus-type-2-polymerase-complex-recognizes-leader-promoters-of-other-species-belonging-to-the-genus-rubulavirus
#5
Yusuke Matsumoto, Keisuke Ohta, Machiko Nishio
Leader sequence, located at the 3' terminus of paramyxovirus genomes, determines the degree of viral transcription and replication. The essential nucleotides in the leader sequence that influence viral propagation, however, have not been investigated in detail. In the present study, we show that polymerase complex of human parainfluenza virus type 2 (hPIV2) uses a luciferase-encoding hPIV2 mini-genome possessing the leader sequence from other closely related viruses as a template. Furthermore, we demonstrate that although hPIV2 polymerase complex can recognize the leader sequence of hPIV4B, mumps virus (MuV) and PIV5 as well as Newcastle disease virus (NDV), it cannot recognize measles virus, hPIV1, Sendai virus (SeV) or hPIV3...
September 7, 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28860488/assessment-of-selected-media-supplements-to-improve-f-hn-lentiviral-vector-production-yields
#6
Jean-François Gélinas, Lee A Davies, Deborah R Gill, Stephen C Hyde
The development of lentiviral-based therapeutics is challenged by the high cost of current Good Manufacturing Practices (cGMP) production. Lentiviruses are enveloped viruses that capture a portion of the host cell membrane during budding, which then constitutes part of the virus particle. This process might lead to lipid and protein depletion in the cell membrane and affect cell viability. Furthermore, growth in suspension also causes stresses that can affect virus production yields. To assess the impact of these issues, selected supplements (Cholesterol Lipid Concentrate, Chemically Defined Lipid Concentrate, Lipid Mixture 1, Gelatin Peptone N3, N-Acetyl-L-Cysteine and Pluronic F-68) were assayed in order to improve production yields in a transient transfection production of a Sendai virus F/HN-pseudotyped HIV-1-based third generation lentiviral vector in FreeStyle 293 (serum-free media) in suspension...
August 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28765300/pronuclear-transplantation-in-the-mouse-embryo
#7
James McGrath, Davor Solter, Jeff Mann
This protocol describes an example of complete zygote enucleation and transplantation of male and female pronuclei; however, single pronuclei can also be removed and transplanted. In this method, pronuclei are removed without penetrating the plasma membrane of the zygote. Instead, they are withdrawn individually or together into a membrane-bound karyoplast that can then be fused with a recipient enucleated zygote using inactivated Sendai virus or electrofusion. Preincubation of the embryos in the presence of the cytoskeletal inhibitors cytochalasin B and colcemid is critical for the survival of the embryos during this microsurgical procedure...
August 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28757035/characterization-of-akabane-virus-from-domestic-bamboo-rat-southern-china
#8
Hai-Bo Tang, Fenglian Chen, Guibo Rao, Anbin Bai, Jiajia Jiang, Yichao Du, Pengfei Ren, Jinfeng Liu, Shaomin Qin, Lei Yang, Jianmin Wu
To identify the causative agents in 3 large-scale outbreaks of encephalitis and death among farmed bamboo rats (Rhizomys pruinosus). The routine bacterial culture and identification were performed. There were no significant pathogenic bacteria isolated from the brain, heart, liver, spleen, lung, or kidney of diseased bamboo rats. Using PCR-based methods, we excluded the following as causative agent: pox virus, herpesvirus, adenovirus, lymphocytic choriomeningitis virus, rabies virus, and sendai virus. Furthermore, the homogenate from the diseased bamboo rats was subjected to viral metagenomic analysis which revealed 48506 filtered viral reads annotated to Akabane virus (AKAV) with >75% nucleotide identity, suggesting the presence of AKAVs in bamboo rats...
August 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28715399/integration-free-derivation-of-human-induced-pluripotent-stem-cells-using-laminin-521-matrix
#9
Elias Uhlin, Ana Marin Navarro, Harriet Rönnholm, Kelly Day, Malin Kele, Anna Falk
Xeno-free and fully defined conditions are key parameters for robust and reproducible generation of homogenous human induced pluripotent stem (hiPS) cells. Maintenance of hiPS cells on feeder cells or undefined matrices are susceptible to batch variances, pathogenic contamination and risk of immunogenicity. Utilizing the defined recombinant human laminin 521 (LN-521) matrix in combination with xeno-free and defined media formulations reduces variability and allows for the consistent generation of hiPS cells...
July 7, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28677537/generation-of-human-induced-pluripotent-stem-cells-from-urinary-cells-of-a-healthy-donor-using-a-non-integration-system
#10
Kyung-Ok Uhm, Eun Hee Jo, Gue Youn Go, So-Jung Kim, Hye Young Choi, Young Sam Im, Hye-Yeong Ha, Ji-Won Jung, Soo Kyung Koo
Urinary cells can be an ideal source for generating hiPSCs and progenitors, as they are easily accessible, non-invasive, and universally available. We generated human induced pluripotent stem cells (hiPSCs) from the urinary cells of a healthy donor using a Sendai virus-based gene delivery method. The generated hiPSC line, KSCBi001-A, has a normal karyotype (46,XY). The pluripotency and capacity of multilineage differentiation were characterized by comparison with those of a human embryonic stem cell line. This cell line is registered and available from National Stem Cell Bank, Korea National Institute of Health...
May 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28677532/establishment-of-an-induced-pluripotent-stem-cell-ipsc-line-from-a-9-year-old-male-with-autism-spectrum-disorder-asd
#11
Eszter Varga, Csilla Nemes, István Bock, Zsuzsanna Táncos, Sára Berzsenyi, György Lévay, Viktor Román, Julianna Kobolák, András Dinnyés
Peripheral blood mononuclear cells (PBMCs) were collected from a clinically characterized patient with autism spectrum disorder (ASD). The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system. The pluripotency of transgene-free iPSCs was verified by immunocytochemistry for pluripotency markers and by spontaneous in vitro differentiation towards the 3 germ layers. Furthermore, the iPSC line showed normal karyotype. Our model might offer a good platform to study the pathomechanism of ASD, also for drug testing, early biomarker discovery and gene therapy studies...
May 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28677531/generation-of-a-human-induced-pluripotent-stem-cell-line-from-urinary-cells-of-a-healthy-donor-using-integration-free-sendai-virus-technology
#12
Bella Rossbach, Laura Hildebrand, Linda El-Ahmad, Harald Stachelscheid, Petra Reinke, Andreas Kurtz
We have generated a human induced pluripotent stem cell (iPSC) line derived from urinary cells of a 28year old healthy female donor. The cells were reprogrammed using a non-integrating viral vector and have shown full differentiation potential. Together with the iPSC line, the donor provided blood cells for the study of immunological effects of the iPSC line and its derivatives in autologous and allogeneic settings. The line is available and registered in the human pluripotent stem cell registry as BCRTi005-A...
May 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28677527/generation-of-human-induced-pluripotent-stem-cell-lines-from-human-dermal-fibroblasts-using-a-non-integration-system
#13
Kyung-Ok Uhm, So-Jung Kim, Eun Hee Jo, Gue Youn Go, Hye Young Choi, Young Sam Im, Hye-Yeong Ha, Jung-Hyun Kim, Soo Kyung Koo
We generated human induced pluripotent stem cells (hiPSCs) from dermal fibroblasts using a Sendai virus (SeV)-based gene delivery method. The generated hiPSC line, KSCBi002-A, has a normal karyotype (46,XY). The pluripotency and differentiation capacity were characterized by comparison with those of a human embryonic stem cell line. This cell line is registered and available from the National Stem Cell Bank, Korea National Institute of Health.
May 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28677526/generation-of-macaca-fascicularis-ips-cell-line-atci-mf1-from-adult-skin-fibroblasts-using-non-integrative-sendai-viruses
#14
Giulia Coppiello, Gloria Abizanda, Natalia Aguado, Elena Iglesias, Estibaliz Arellano-Viera, Juan R Rodriguez-Madoz, Xonia Carvajal-Vergara, Felipe Prosper, Xabier L Aranguren
We generated ATCi-MF1 induced pluripotent stem (iPS) cell line from Macaca fascicularis adult skin fibroblasts using non-integrative Sendai viruses carrying OCT3/4, KLF4, SOX2 and c-MYC. Once established, ATCi-MF1 cells present a normal karyotype, are Sendai virus-free and express pluripotency associated markers. Microsatellite markers analysis confirmed the origin of the iPS cells from the parental fibroblasts. Pluripotency was tested with the in vivo teratoma formation assay. ATCi-MF1 cell line may be a useful primate iPS cell model to test different experimental conditions where the use of human cells can imply ethical issues, as microinjection of pluripotent stem cells in pre-implantational embryos...
May 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28675011/-characterization-of-micrornas-profiles-of-induced-pluripotent-stem-cells-reprogrammed-from-human-dental-pulp-stem-cells-and-stem-cells-from-apical-papilla
#15
Tan Xiaobing, Dai Qingyuan
OBJECTIVE: To compare characterization of microRNAs (miRNAs) expression profiles of induced pluripotent stem cells (iPSCs) reprogrammed from human dental pulp stem cells (DPSCs) and stem cells from apical papilla (SCAP) and screen-specific microRNA. METHODS: Human DPSCs and SCAP were reprogrammed into iPSCs using a Sendai virus vector. Total RNA of human DPSCs-iPSCs and SCAP-iPSCs were extracted. miRNAs were labeled and hybridized. Slides were scanned, and images were imported into GenePix Pro 6...
June 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/28666145/simple-and-effective-generation-of-transgene-free-induced-pluripotent-stem-cells-using-an-auto-erasable-sendai-virus-vector-responding-to-microrna-302
#16
Ken Nishimura, Manami Ohtaka, Hitomi Takada, Akira Kurisaki, Nhi Vo Kieu Tran, Yen Thi Hai Tran, Koji Hisatake, Masayuki Sano, Mahito Nakanishi
Transgene-free induced pluripotent stem cells (iPSCs) are valuable for both basic research and potential clinical applications. We previously reported that a replication-defective and persistent Sendai virus (SeVdp) vector harboring four reprogramming factors (SeVdp-iPS) can efficiently induce generation of transgene-free iPSCs. This vector can express all four factors stably and simultaneously without chromosomal integration and can be eliminated completely from reprogrammed cells by suppressing vector-derived RNA-dependent RNA polymerase...
August 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28659477/west-nile-virus-ns1-antagonizes-interferon-beta-production-by-targeting-rig-i-and-mda5
#17
Hong-Lei Zhang, Han-Qing Ye, Si-Qing Liu, Cheng-Lin Deng, Xiao-Dan Li, Pei-Yong Shi, Bo Zhang
West Nile virus (WNV) is a mosquito-borne flavivirus that causes epidemics of encephalitis and viscerotropic disease worldwide. This virus has spread rapidly and has posed a significant public health threat since the outbreak in New York City in 1999. The interferon (IFN)-mediated antiviral response represents an important component of virus-host interactions and plays an essential role in regulating viral replication. Previous studies have suggested that multifunctional nonstructural proteins encoded by flaviviruses antagonize the host IFN response via various means in order to establish efficient viral replication...
September 15, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28636653/the-ebola-virus-vp35-protein-binds-viral-immunostimulatory-and-host-rnas-identified-through-deep-sequencing
#18
Kari A Dilley, Alexander A Voorhies, Priya Luthra, Vinita Puri, Timothy B Stockwell, Hernan Lorenzi, Christopher F Basler, Reed S Shabman
Ebola virus and Marburg virus are members of the Filovirdae family and causative agents of hemorrhagic fever with high fatality rates in humans. Filovirus virulence is partially attributed to the VP35 protein, a well-characterized inhibitor of the RIG-I-like receptor pathway that triggers the antiviral interferon (IFN) response. Prior work demonstrates the ability of VP35 to block potent RIG-I activators, such as Sendai virus (SeV), and this IFN-antagonist activity is directly correlated with its ability to bind RNA...
2017: PloS One
https://www.readbyqxmd.com/read/28631605/loss-of-sendai-virus-c-protein-leads-to-accumulation-of-rig-i-immunostimulatory-defective-interfering-rna
#19
Maria Teresa Sánchez-Aparicio, Dominique Garcin, Charles M Rice, Daniel Kolakofsky, Adolfo García-Sastre, Alina Baum
Retinoic acid inducible gene (RIG-I)-mediated innate immunity plays a pivotal role in defence against virus infections. Previously we have shown that Sendai virus (SeV) defective interfering (DI) RNA functions as an exclusive and potent RIG-I ligand in DI-RNA-rich SeV-Cantell infected cells. To further understand how RIG-I is activated during SeV infection, we used a different interferon (IFN)-inducing SeV strain, recombinant SeVΔC, which, in contrast to SeV-Cantell is believed to stimulate IFN production due to the lack of the SeV IFN antagonist protein C...
June 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28605636/a-sendai-virus-recombinant-vaccine-expressing-a-gene-for-truncated-human-metapneumovirus-hmpv-fusion-protein-protects-cotton-rats-from-hmpv-challenge
#20
Charles J Russell, Bart G Jones, Robert E Sealy, Sherri L Surman, John N Mason, Randall T Hayden, Ralph A Tripp, Toru Takimoto, Julia L Hurwitz
Human metapneumovirus (hMPV) infections pose a serious health risk to young children, particularly in cases of premature birth. No licensed vaccine exists and there is no standard treatment for hMPV infections apart from supportive hospital care. We describe the production of a Sendai virus (SeV) recombinant that carries a gene for a truncated hMPV fusion (F) protein (SeV-MPV-Ft). The vaccine induces binding and neutralizing antibody responses toward hMPV and protection against challenge with hMPV in a cotton rat system...
September 2017: Virology
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