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https://www.readbyqxmd.com/read/28508216/rescue-of-sendai-virus-from-cloned-cdna
#1
Shringkhala Bajimaya, Tsuyoshi Hayashi, Toru Takimoto
Sendai virus (SeV) is a non-segment negative-sense RNA virus that naturally infects and causes pneumonia in mice. As a prototypic member of the family Paramyxoviridae, SeV has been characterized well, and these studies revealed numerous traits of paramyxovirus biology. The reverse genetics system to rescue SeV was first established in 1995. The virus was rescued from a cloned cDNA that contains full genome sequence flanked by T7 promoter and hepatitis delta virus ribozyme. To rescue SeV, it is necessary to infect cells with a recombinant vaccinia virus vTF7...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28498811/the-generation-and-functional-characterization-of-induced-pluripotent-stem-cells-from-human-intervertebral-disc-nucleus-pulposus-cells
#2
Yanxia Zhu, Yuhong Liang, Hongxia Zhu, Cuihong Lian, Liang Wang, Yiwei Wang, Hongsheng Gu, Guangqian Zhou, Xiaoping Yu
Disc degenerative disease (DDD) is believed to originate in the nucleus pulposus (NP) region therefore, it is important to obtain a greater number of active NP cells for the study and therapy of DDD. Human induced pluripotent stem cells (iPSCs) are a powerful tool for modeling the development of DDD in humans, and have the potential to be applied in regenerative medicine. NP cells were isolated from DDD patients following our improved method, and then the primary NP cells were reprogramed into iPSCs with Sendai virus vectors encoding 4 factors...
April 26, 2017: Oncotarget
https://www.readbyqxmd.com/read/28497777/phase-i-ii-clinical-trial-to-assess-safety-and-efficacy-of-intratumoral-and-subcutaneous-injection-of-hvj-e-in-castration-resistant-prostate-cancer-patients
#3
K Fujita, Y Nakai, A Kawashima, T Ujike, A Nagahara, T Nakajima, T Inoue, C M Lee, M Uemura, Y Miyagawa, Y Kaneda, N Nonomura
Inactivated Sendai virus particles (hemagglutinating virus of Japan envelope (HVJ-E)) have a novel antitumor effect: HVJ-E fused to prostate cancer cells via cell surface receptor causes apoptosis of prostate cancer cells in vitro and in vivo. HVJ-E also induces antitumor immunity by activating natural killer (NK) cells and cytotoxic T cells and suppressing regulatory T cells in vivo. We conducted an open-label, single-arm, phase I/II clinical trial in patients with castration-resistant prostate cancer (CRPC) to determine the safety and efficacy of intratumoral and subcutaneous injection of HVJ-E...
May 12, 2017: Cancer Gene Therapy
https://www.readbyqxmd.com/read/28490810/establishment-of-induced-pluripotent-stem-cells-from-normal-b-cells-and-inducing-aid-expression-in-their-differentiation-into-hematopoietic-progenitor-cells
#4
Fumihiko Kawamura, Makoto Inaki, Atsushi Katafuchi, Yu Abe, Naohiro Tsuyama, Yumiko Kurosu, Aki Yanagi, Mitsunori Higuchi, Satoshi Muto, Takumi Yamaura, Hiroyuki Suzuki, Hideyoshi Noji, Shinichi Suzuki, Mitsuaki A Yoshida, Megumi Sasatani, Kenji Kamiya, Masafumi Onodera, Akira Sakai
B cell derived induced pluripotent stem cells (BiPSCs) were recently established from peripheral blood B cells by the simultaneous transfection of Yamanaka factors (Oct3/4, Sox2, Klf4, c-Myc) and C/EBPα using a Sendai virus vector. Here, using a different method, we established BiPSCs with immunoglobulin heavy chain (IgH) gene rearrangement from normal B cells purified from lymph nodes. The critical points of our method are pre-stimulation of B cells with IL-21 and CD40-ligand (CD40L), followed by consecutive transfection of highly concentrated Yamanaka factors using a retroviral vector...
May 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28487378/encephalomyocarditis-virus-3c-protease-attenuates-type-i-interferon-production-through-disrupting-tank-tbk1-ikk%C3%AE%C2%B5-irf3-complex
#5
Li Huang, Tao Xiong, Huibin Yu, Quan Zhang, Kunli Zhang, Changyao Li, Liang Hu, Yuanfeng Zhang, Lijie Zhang, Qinfang Liu, Shengnan Wang, Xijun He, Zhigao Bu, Xuehui Cai, Shangjin Cui, Jiangnan Li, Changjiang Weng
TRAF family member-associated NF-κB activator (TANK) is a scaffold protein that assembles into the interferon regulator factor 3 (IRF3)-phosphorylating TBK1-IKKε complex, where it is involved in regulating phosphorylation of the IRF3 and interferon (IFN) production. However, the functions of TANK in Encephalomyocarditis virus (EMCV) infection-induced type I IFN production are not fully understood. Here, we demonstrated that, instead of stimulating type I IFN production, the EMCV-HB10 strain infection potently inhibited Sendai virus (SeV)- and polyI:C-induced IRF3 phosphorylation and type I IFN production in HEK293T cells...
May 9, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28474501/pol7085-or-anti-ccl28-treatment-inhibits-development-of-post-paramyxoviral-airway-disease
#6
Becky J Buelow, Michelle Rohlfing, Françoise Jung, Garry J Douglas, Mitchell H Grayson
INTRODUCTION: Asthma is major health burden throughout the world, and there are no therapies that have been shown to be able to prevent the development of disease. A severe respiratory paramyxoviral infection early in life has been demonstrated to greatly increase the risk of developing asthma. We have a mouse model of a severe respiratory paramyxoviral infection (Sendai virus, SeV) that mimics human disease, and requires early expression of the cytokine CCL28 to drive the development of post-viral airway disease...
June 2017: Immunity, Inflammation and Disease
https://www.readbyqxmd.com/read/28465093/generation-genome-edition-and-characterization-of-ipsc-lines-from-a-patient-with-coenzyme-q10-deficiency-harboring-a-heterozygous-mutation-in-coq4-gene
#7
Damià Romero-Moya, Julio Castaño, Carlos Santos-Ocaña, Plácido Navas, Pablo Menendez
We report the generation, CRISPR/Cas9-edition and characterization of induced pluripotent stem cell (iPSC) lines from a patient with coenzyme Q10 deficiency harboring the heterozygous mutation c.483G>C in the COQ4 gene. iPSCs were generated using non-integrative Sendai Viruses containing the reprogramming factors OCT4, SOX2, KLF4 and C-MYC. The iPSC lines carried the c.483G>C COQ4 mutation, silenced the OKSM expression and were mycoplasma-free. They were bona fide pluripotent cells as characterized by morphology, immunophenotype/gene expression for pluripotent-associated markers/genes, NANOG and OCT4 promoter demethylation, karyotype and teratoma formation...
September 16, 2016: Stem Cell Research
https://www.readbyqxmd.com/read/28435159/ifitm3-inhibits-virus-triggered-induction-of-type-i-interferon-by-mediating-autophagosome-dependent-degradation-of-irf3
#8
Li-Qun Jiang, Tian Xia, Yun-Hong Hu, Ming-Shun Sun, Shuang Yan, Cao-Qi Lei, Hong-Bing Shu, Ji-Hua Guo, Yu Liu
Interferon-induced transmembrane protein 3 (IFITM3) is a restriction factor that can be induced by viral infection and interferons (IFNs). It inhibits the entry and replication of many viruses, which are independent of receptor usage but dependent on processes that occur in endosomes. In this study, we demonstrate that IFITM3 plays important roles in regulating the RNA-virus-triggered production of IFN-β in a negative-feedback manner. Overexpression of IFITM3 inhibited Sendai virus-triggered induction of IFN-β, whereas knockdown of IFITM3 had the opposite effect...
April 24, 2017: Cellular & Molecular Immunology
https://www.readbyqxmd.com/read/28413006/generation-of-a-human-induced-pluripotent-stem-cell-ipsc-line-from-a-64year-old-male-patient-with-multiple-schwannoma
#9
Shaokun Zhang, Zhenshan Lv, Yang Hu, Lidi Liu, Weiquan Gong, Qiao Li, Hong Wu
Peripheral blood was collected from a clinically diagnosed 64-year old male multiple schwannoma patient. Peripheral blood mononuclear cells (PBMCs) were reprogrammed with the Yamanaka KMOS reprogramming factors using the Sendai-virus reprogramming system. The transgene-free iPSC line showed pluripotency verified by immunofluorescent staining for pluripotency markers, and the iPSC line was able to differentiate into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This in vitro cellular model will be useful for further pathological studies of multiple schwannoma...
March 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28413004/derivation-of-human-induced-pluripotent-stem-cell-ipsc-line-from-a-79year-old-sporadic-male-parkinson-s-disease-patient
#10
Shaokun Zhang, Lidi Liu, Yang Hu, Zhenshan Lv, Qiao Li, Weiquan Gong, Hui Sha, Hong Wu
Peripheral blood was collected from a clinically diagnosed 79-year old male sporadic Parkinson's disease patient. Peripheral blood mononuclear cells (PBMCs) were reprogrammed with the Yamanaka KMOS reprogramming factors using the Sendai-virus reprogramming system. The transgene-free iPSC line showed pluripotency verified by immunofluorescent staining for pluripotency markers, and the iPSC line was able to differentiate into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This in vitro cellular model can be used to study the mechanism of sporadic Parkinson's disease and to test new drugs...
March 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28413002/characterization-of-human-induced-pluripotent-stem-cell-ipsc-line-from-a-72year-old-male-patient-with-later-onset-alzheimer-s-disease
#11
Shaokun Zhang, Zhenshan Lv, Songyuan Zhang, Lidi Liu, Qiao Li, Weiquan Gong, Hui Sha, Hong Wu
Peripheral blood was collected from a clinically diagnosed 72-year old male patient with later onset Alzheimer's disease. Peripheral blood mononuclear cells (PBMCs) were reprogrammed with the Yamanaka KMOS reprogramming factors using the Sendai-virus reprogramming system. The transgene-free iPSC line showed pluripotency verified by immunofluorescent staining for pluripotency markers, and the iPSC line was able to differentiate into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This in vitro cellular model will be useful for studying the pathological mechanism of Alzheimer's disease...
March 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28413001/development-of-human-induced-pluripotent-stem-cell-ipsc-line-from-a-60year-old-female-patient-with-multiple-schwannoma
#12
Shaokun Zhang, Zhenshan Lv, Yan Liu, Qiao Li, Weiquan Gong, Lidi Liu, Hong Wu
Peripheral blood was collected from a clinically diagnosed 60-year old female patient with multiple schwannoma. Peripheral blood mononuclear cells (PBMCs) were reprogrammed with the Yamanaka KMOS reprogramming factors using the Sendai-virus reprogramming system. The transgene-free iPSC line showed pluripotency verified by immunofluorescent staining for pluripotency markers, and the iPSC line was able to differentiate into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This in vitro cellular model will be useful for further pathological studies of multiple schwannoma...
March 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28412134/clinical-courses-of-herpes-simplex-virus-induced-urethritis-in-men
#13
Shin Ito, Mitsuru Yasuda, Hiromi Kondo, Yoshiteru Yamada, Keita Nakane, Kosuke Mizutani, Tomohiro Tsuchiya, Shigeaki Yokoi, Masahiro Nakano, Takashi Deguchi
We retrieved clinical data of 13 men having herpes simplex virus (HSV)-induced non-gonococcal urethritis (NGU) without visible herpetic lesions. They visited a clinic in Sendai, Japan, between April 2013 and December 2015. All the men complained of dysuria. Meatitis was observed in 9 of the 13 men. Mononuclear cells were observed in the urethral smears from 9 men. The 13 men were treated with azithromycin or sitafloxacin regimen. First-voided urine (FVU) specimens became negative for HSV in 8 of the 10 men who returned to the clinic after antibacterial treatment, and urethritis symptoms were alleviated...
April 12, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28401936/bmal1-links-the-circadian-clock-to-viral-airway-pathology-and-asthma-phenotypes
#14
A Ehlers, W Xie, E Agapov, S Brown, D Steinberg, R Tidwell, G Sajol, R Schutz, R Weaver, H Yu, M Castro, L B Bacharier, X Wang, M J Holtzman, J A Haspel
Patients with asthma experience circadian variations in their symptoms. However it remains unclear how specific aspects of this common airway disease relate to clock genes, which are critical to the generation of circadian rhythms in mammals. Here, we used a viral model of acute and chronic airway disease to examine how circadian clock disruption affects asthmatic lung phenotypes. Deletion of the core clock gene bmal1 or environmental disruption of circadian function by jet lag exacerbated acute viral bronchiolitis caused by Sendai virus (SeV) and influenza A virus in mice...
April 12, 2017: Mucosal Immunology
https://www.readbyqxmd.com/read/28395805/generation-of-a-human-induced-pluripotent-stem-cell-ipsc-line-carrying-the-parkinson-s-disease-linked-lrrk2-variant-s1647t
#15
Dongrui Ma, Shin Hui Ng, Li Zeng, Yi Zhao, Eng King Tan
Peripheral blood mononuclear cells (PBMCs) were collected from a clinically diagnosed 64-year old male Parkinson's disease (PD) patient with S1647T variant in the LRRK2 gene. The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus reprogramming system. The transgene-free iPSC showed pluripotency confirmed by immunofluorescent staining for pluripotency markers and differentiated into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This cellular model will be useful for further function studies and therapeutic screening...
January 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395804/development-of-a-human-induced-pluripotent-stem-cell-ipsc-line-from-a-parkinson-s-disease-patient-carrying-the-n551k-variant-in-lrrk2-gene
#16
Dongrui Ma, Ebonne Yulin Ng, Li Zeng, Christina Ying Yan Lim, Yi Zhao, Eng King Tan
Peripheral blood mononuclear cells (PBMCs) were collected from a clinically diagnosed 64-year old male Parkinson's disease (PD) patient with N551K variant in the LRRK2 gene. The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus reprogramming system. The transgene-free iPSC showed pluripotency confirmed by immunofluorescent staining for pluripotency markers and differentiated into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This cellular model can complement in vivo PD models for pathophysiological studies and drug screening...
January 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395803/derivation-of-human-induced-pluripotent-stem-cell-ipsc-line-with-lrrk2-gene-r1398h-variant-in-parkinson-s-disease
#17
Dongrui Ma, Murni Tio, Shin Hui Ng, Li Zeng, Christina Ying Yan Lim, Yi Zhao, Eng King Tan
Peripheral blood mononuclear cells (PBMCs) were collected from a clinically diagnosed 72-year old female Parkinson's disease (PD) patient with R1398H variant in the LRRK2 gene. The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus reprogramming system. The transgene-free iPSC showed pluripotency confirmed by immunofluorescent staining for pluripotency markers and differentiated into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This cellular model provides a good platform for studying the mechanism of PD, and also for drug testing and gene therapy studies...
January 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395802/reprogramming-of-a-human-induced-pluripotent-stem-cell-ipsc-line-from-a-parkinson-s-disease-patient-with-a-r1628p-variant-in-the-lrrk2-gene
#18
Dongrui Ma, Wei Zhou, Ebonne Yulin Ng, Li Zeng, Yi Zhao, Eng King Tan
Peripheral blood mononuclear cells (PBMCs) were collected from a clinically diagnosed 59-year old male Parkinson's disease (PD) patient with R1628P variant in the LRRK2 gene. The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus reprogramming system. The transgene-free iPSC showed pluripotency confirmed by immunofluorescent staining for pluripotency markers and differentiated into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This cellular model will provide a good resource for further pathophysiological studies of PD...
January 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395801/generation-of-human-ipscs-from-urine-derived-cells-of-patient-with-a-novel-heterozygous-pai-1-mutation
#19
Muhammad Zeeshan Afzal, Melanie Gartz, Ekaterina A Klyachko, Sadiya Sana Khan, Sanjiv J Shah, Sweta Gupta, Amy D Shapiro, Douglas E Vaughan, Jennifer L Strande
We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subjects heterozygous for a novel Plasminogen Activator Inhibitor-1 (PAI-1) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the PAI-1 UCs.
January 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395799/generation-of-human-ipscs-from-urine-derived-cells-of-a-non-affected-control-subject
#20
Muhammad Zeeshan Afzal, Melanie Gartz, Ekaterina A Klyachko, Sadiya Sana Khan, Sanjiv J Shah, Sweta Gupta, Amy D Shapiro, Douglas E Vaughan, Jennifer L Strande
We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from non-affected control subjects to use as a comparison group for the iPSC lines containing a Plasminogen Activator Inhibitor-1 (PAI-1 homozygous/heterozygous) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the UCs.
January 2017: Stem Cell Research
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