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https://www.readbyqxmd.com/read/29452390/effects-of-storage-conditions-on-hatchability-embryonic-survival-and-cytoarchitectural-properties-in-broiler-from-young-and-old-flocks
#1
N Pokhrel, E Ben-Tal Cohen, O Genin, M Ruzal, D Sela-Donenfeld, Y Cinnamon
Storing eggs at low temperature prior to incubation is common practice in the broiler hatchery industry; however, prolonged storage (beyond 7 d) is known to increase early embryonic mortality and reduce chick quality and performance. To better understand the basis of this mortality, we previously published milestone criteria to evaluate morphological and cellular properties of the freshly laid embryo. Using these criteria, in the present study we checked the effects of storage at 18°C and 12°C for up to 28 d on hatchability and chick quality...
February 14, 2018: Poultry Science
https://www.readbyqxmd.com/read/29451576/ortho-chlorination-of-phenoxy-1-2-dioxetane-yields-superior-chemiluminescent-probes-for-in-vitro-and-in-vivo-imaging
#2
Tal Eilon-Shaffer, Michal Roth-Konforti, Anat Eldar-Boock, Ronit Satchi-Fainaro, Doron Shabat
A recent methodology, developed by our group, has enabled a dramatic improvement in the emissive nature of the excited species, formed during the chemiexcitation of dioxetanes under physiological conditions. This approach has resulted in the discovery of distinct phenoxy-dioxetane luminophores that produce a chemiluminescence signal via a direct-mode of emission. Here, we show a significant pK a effect of our new phenoxy-dioxetanes on their chemiexcitation and on their ability to serve as chemiluminescent turn-ON probes for biological applications...
February 16, 2018: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/29451138/detection-of-repair-of-the-zone-of-calcified-cartilage-with-osteoarthritis-through-mesenchymal-stem-cells-by-ultrashort-echo-time-magnetic-resonance-imaging
#3
REVIEW
Quan Zhou, Shao-Lin Li, Ya-Jun Ma, Vicki de Tal, Wei Li, Ying-Hua Zhao
Objective: Currently, magnetic resonance imaging (MRI) is the most commonly used imaging modality for observing the growth and development of mesenchymal stem cells (MSCs) after in vivo transplantation to treat osteoarthritis (OA). However, it is a challenge to accurately monitor the treatment effects of MSCs in the zone of calcified cartilage (ZCC) with OA. This is especially true in the physiological and biochemical views that are not accurately detected by MRI contrast agents. In contrast, ultrashort time echo (UTE) MRI has been shown to be sensitive to the presence of the ZCC, creating the potential for more effectively observing the repair of the ZCC in OA by MSCs...
February 5, 2018: Chinese Medical Journal
https://www.readbyqxmd.com/read/29450941/hematogenous-dissemination-of-mesenchymal-stem-cells-from-endometriosis
#4
Fei Li, Myles H Alderman, Aya Tal, Ramanaiah Mamillapalli, Alexis Coolidge, Demetra Hufnagel, Zhihao Wang, Elham Neisani, Stephanie Gidicsin, Graciela Krikun, Hugh S Taylor
Endometriosis is ectopic growth of endometrial tissue traditionally thought to arise through retrograde menstruation. We aimed to determine if cells derived from endometriosis could enter vascular circulation and lead to hematogenous dissemination. Experimental endometriosis was established by transplanting endometrial tissue from DsRed+ mice into the peritoneal cavity of DsRed- mice. Using flow cytometry, we identified DsRed+ cells in blood of animals with endometriosis. The circulating donor cells expressed CXCR4 and mesenchymal stem cell (MSC) biomarkers, but not hematopoietic stem cell markers...
February 16, 2018: Stem Cells
https://www.readbyqxmd.com/read/29450710/indicators-for-imaging-in-periorbital-cellulitis-secondary-to-rhinosinusitis
#5
Basel Jabarin, Ephraim Eviatar, Ofer Israel, Tal Marom, Haim Gavriel
PURPOSE: Our objective was to identify the clinical indicators for subperiosteal orbital abscess (SPOA) among patients who present with periorbital cellulitis secondary to rhinosinusitis, and to evaluate the need for performing a computerized tomography (CT) scan. METHODS: A retrospective cohort study of all patients admitted to our tertiary care emergency department between 2006 and 2014 was conducted. Included were healthy patients with acute periorbital cellulitis secondary to rhinosinusitis...
February 15, 2018: European Archives of Oto-rhino-laryngology
https://www.readbyqxmd.com/read/29450676/comorbid-obstructive-sleep-apnea-and-increased-risk-for-sickle-cell-disease-morbidity
#6
Tal Katz, Jeffrey Schatz, Carla W Roberts
PURPOSE: Sickle cell disease (SCD) imparts an increased risk for obstructive sleep apnea (OSA) in childhood. Studies of pediatric SCD have identified an increased risk for pain and neurologic complications with comorbid OSA. We determined the rate of a broad range of SCD-related medical complications to better characterize the spectrum of SCD complications related to OSA. METHODS: Retrospective chart review at a single hematology clinic identified 641 youth with SCD who received consistent screenings for OSA as part of routine hematological health maintenance visits over an 11-year period...
February 15, 2018: Sleep & Breathing, Schlaf & Atmung
https://www.readbyqxmd.com/read/29444812/classifying-heterotaxy-syndrome-time-for-a-new-approach
#7
EDITORIAL
Stephen P Sanders, Tal Geva
No abstract text is available yet for this article.
February 2018: Circulation. Cardiovascular Imaging
https://www.readbyqxmd.com/read/29441069/interleukin-6-and-interferon-%C3%AE-signaling-via-jak1-stat-differentially-regulate-oncolytic-versus-cytoprotective-antiviral-states
#8
Oded Danziger, Tal Pupko, Eran Bacharach, Marcelo Ehrlich
Malignancy-induced alterations to cytokine signaling in tumor cells differentially regulate their interactions with the immune system and oncolytic viruses. The abundance of inflammatory cytokines in the tumor microenvironment suggests that such signaling plays key roles in tumor development and therapy efficacy. The JAK-STAT axis transduces signals of interleukin-6 (IL-6) and interferons (IFNs), mediates antiviral responses, and is frequently altered in prostate cancer (PCa) cells. However, how activation of JAK-STAT signaling with different cytokines regulates interactions between oncolytic viruses and PCa cells is not known...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29440256/identification-of-streptococcus-gallolyticus-subsp-gallolyticus-biotype-i-competence-stimulating-peptide-pheromone
#9
Anthony Harrington, Yftah Tal-Gan
Streptococcus gallolyticus subsp. gallolyticus ( Sgg ), a member of the group D streptococci, is normally found in the bovine rumen and human gut. It is an opportunistic pathogen that was recently determined to be a bacterial driver of colorectal cancer in addition to causing other diseases such as infective endocarditis, bacteremia, neonatal meningitis, and septicemia. As an emerging pathogen, not much is known about this bacterium, its virulence mechanisms, or its virulence regulatory pathways. Previous studies suggest that Sgg uses a ComRS pathway for competence, one of many Streptococcus quorum sensing circuitries...
February 12, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29438273/novel-phage-display-derived-anti-abrin-antibodies-confer-post-exposure-protection-against-abrin-intoxication
#10
Adva Mechaly, Ron Alcalay, Tal Noy-Porat, Eyal Epstein, Yoav Gal, Ohad Mazor
Abrin toxin is a type 2 ribosome inactivating glycoprotein isolated from the seeds of Abrus precatorius (jequirity pea). Owing to its high toxicity, relative ease of purification and accessibility, it is considered a biological threat agent. To date, there is no effective post-exposure treatment for abrin poisoning and passive immunization remains the most effective therapy. However, the effectiveness of anti-abrin monoclonal antibodies for post-exposure therapy following abrin intoxication has not been demonstrated...
February 13, 2018: Toxins
https://www.readbyqxmd.com/read/29437944/emphysema-and-extrapulmonary-tissue-loss-in-copd-a-multi-organ-loss-of-tissue-phenotype
#11
Bartolome R Celli, Nicholas Locantore, Ruth Tal-Singer, John Riley, Bruce Miller, Jørgen Vestbo, Julie C Yates, Edwin K Silverman, Caroline A Owen, Miguel Divo, Victor Pinto-Plata, Emiel F M Wouters, Rosa Faner, Alvar Agusti
We tested whether emphysema progression accompanies enhanced tissue loss in other body compartments in 1817 patients from the ECLIPSE chronic obstructive pulmonary disease (COPD) cohort.Clinical and selected systemic biomarker measurements were compared in subjects grouped by quantitative tomography scan emphysema quartiles using the percentage of low attenuation area (LAA%). Lowest and highest quartile patients had amino-acid metabolomic profiles. We related LAA% to 3 years decline in lung function (forced expiratory volume in 1 s (FEV 1 )), body mass index (BMI), fat-free mass index (FFMI) and exacerbations, hospitalisations and mortality rates...
February 2018: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/29437616/a-novel-rabbit-spirometry-model-of-type-e-botulism-and-its-use-for-the-evaluation-of-post-symptom-antitoxin-efficacy
#12
Eran Diamant, Avi Pass, Osnat Rosen, Alon Ben David, Amram Torgeman, Ada Barnea, Arnon Tal, Amir Rosner, Ran Zichel
Botulinum neurotoxins (BoNTs), the most poisonous substances known in nature, pose significant concern to health authorities. The only approved therapeutic for botulism is antitoxin. While administered to patients only after symptom onset, antitoxin efficacy is evaluated in animals mostly in relation to time post-intoxication regardless of symptoms. This is most likely due to the difficulty to measure early symptoms of botulism in animals. In the current study, a rabbit spirometry model was developed to quantify early respiratory symptoms of type E botulism that were further used as trigger for treatment...
February 5, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29434652/the-open-abdomen-in-trauma-and-non-trauma-patients-wses-guidelines
#13
REVIEW
Federico Coccolini, Derek Roberts, Luca Ansaloni, Rao Ivatury, Emiliano Gamberini, Yoram Kluger, Ernest E Moore, Raul Coimbra, Andrew W Kirkpatrick, Bruno M Pereira, Giulia Montori, Marco Ceresoli, Fikri M Abu-Zidan, Massimo Sartelli, George Velmahos, Gustavo Pereira Fraga, Ari Leppaniemi, Matti Tolonen, Joseph Galante, Tarek Razek, Ron Maier, Miklosh Bala, Boris Sakakushev, Vladimir Khokha, Manu Malbrain, Vanni Agnoletti, Andrew Peitzman, Zaza Demetrashvili, Michael Sugrue, Salomone Di Saverio, Ingo Martzi, Kjetil Soreide, Walter Biffl, Paula Ferrada, Neil Parry, Philippe Montravers, Rita Maria Melotti, Francesco Salvetti, Tino M Valetti, Thomas Scalea, Osvaldo Chiara, Stefania Cimbanassi, Jeffry L Kashuk, Martha Larrea, Juan Alberto Martinez Hernandez, Heng-Fu Lin, Mircea Chirica, Catherine Arvieux, Camilla Bing, Tal Horer, Belinda De Simone, Peter Masiakos, Viktor Reva, Nicola DeAngelis, Kaoru Kike, Zsolt J Balogh, Paola Fugazzola, Matteo Tomasoni, Rifat Latifi, Noel Naidoo, Dieter Weber, Lauri Handolin, Kenji Inaba, Andreas Hecker, Yuan Kuo-Ching, Carlos A Ordoñez, Sandro Rizoli, Carlos Augusto Gomes, Marc De Moya, Imtiaz Wani, Alain Chichom Mefire, Ken Boffard, Lena Napolitano, Fausto Catena
Damage control resuscitation may lead to postoperative intra-abdominal hypertension or abdominal compartment syndrome. These conditions may result in a vicious, self-perpetuating cycle leading to severe physiologic derangements and multiorgan failure unless interrupted by abdominal (surgical or other) decompression. Further, in some clinical situations, the abdomen cannot be closed due to the visceral edema, the inability to control the compelling source of infection or the necessity to re-explore (as a "planned second-look" laparotomy) or complete previously initiated damage control procedures or in cases of abdominal wall disruption...
2018: World Journal of Emergency Surgery: WJES
https://www.readbyqxmd.com/read/29434619/from-bioengineering-to-crispr-cas9-a-personal-retrospective-of-20-years-of-research-in-programmable-genome-targeting
#14
Albert Jeltsch
Genome targeting of restriction enzymes and DNA methyltransferases has many important applications including genome and epigenome editing. 15-20 years ago, my group was involved in the development of approaches for programmable genome targeting, aiming to connect enzymes with an oligodeoxynucleotide (ODN), which could form a sequence-specific triple helix at the genomic target site. Importantly, the target site of such enzyme-ODN conjugate could be varied simply by altering the ODN sequence promising great applicative values...
2018: Frontiers in Genetics
https://www.readbyqxmd.com/read/29431303/eosinophilic-fasciitis-a-single-center-experience-of-seven-patients
#15
Oded Shamriz, Mariana Druker, Tzahi Neuman, Zvi Dranitzki, Yuval Tal
BACKGROUND: Eosinophilic fasciitis (EF) is a rare disease characterized by scleroderma-like skin, inflammation of deep muscle fascia, hypergammaglobulinemia, peripheral eosinophilia, and elevated erythrocyte sedimentation rate. OBJECTIVES: To present our experience in diagnosis and treatment of seven biopsy-proven EF patients in a large tertiary medical center. METHODS: We screened all patients who were admitted to our tertiary medical center and diagnosed with EF by tissue biopsies from January 2000 to January 2016...
February 2018: Israel Medical Association Journal: IMAJ
https://www.readbyqxmd.com/read/29426379/success-in-adults-with-probable-developmental-coordination-disorder-using-structural-equation-modeling
#16
Miri Tal-Saban, Asher Ornoy, Shula Parush
OBJECTIVE: The aim of this study was to conduct a broad in-depth examination of the relationship between developmental coordination disorder (DCD) and use of executive strategies, daily life activities, emotional state, and internal factors attributed to success. METHOD: We used a large randomized cohort of young adults. Structural equation modeling was performed to test both the direct effects and the mediated effects of the independent variable (probable DCD) on dependent variables (executive functions, academic and nonacademic activities, emotional state, and attribution to success)...
March 2018: American Journal of Occupational Therapy: Official Publication of the American Occupational Therapy Association
https://www.readbyqxmd.com/read/29426038/effect-of-short-term-training-on-fine-motor-control-in-trigeminally-innervated-versus-spinally-innervated-muscles
#17
Hong Zhang, Abhishek Kumar, Xiaoping Luo, Krister Svensson, Mats Trulsson, Peter Svensson
We hypothesized that the trigeminally innervated jaw muscles and spinally innervated hand muscles would differ in the force control and muscle activity when similar fine motor training tasks are performed. Sixteen healthy volunteers performed six series (with ten trials each) of an oral fine motor task (OFMT) and a hand fine motor task (HFMT), in random order. The task was to hold-and-break a test material (5 cm spaghetti pasta) placed on the force transducer between either their anterior teeth (OFMT) or the thumb and the index finger (HFMT)...
February 6, 2018: Human Movement Science
https://www.readbyqxmd.com/read/29425836/the-impact-of-a-daily-smartphone-based-feedback-system-among-women-with-gestational-diabetes-on-compliance-glycemic-control-satisfaction-and-pregnancy-outcome-a-randomized-controlled-trial
#18
Hadas Miremberg, Tal Ben-Ari, Tal Betzer, Hagit Raphaeli, Rose Gasnier, Giulia Barda, Jacob Bar, Eran Weiner
BACKGROUND: Patient compliance and tight glycemic control have been demonstrated to improve pregnancy outcome in pregnancies complicated by gestational diabetes mellitus (GDM). The use of advanced technological tools, including smartphone-based platforms, to improve medical care and outcomes has been demonstrated in various fields of medicine, but only few small studies were performed with GDM patients. OBJECTIVE: We aimed to study the impact of introducing a smartphone-based daily feedback and communication platform between GDM patients and their physicians, on patient compliance, glycemic control, pregnancy outcome, and patient satisfaction...
February 6, 2018: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/29421235/analysis-of-rat-cardiac-myocytes-and-fibroblasts-identifies-combinatorial-enhancer-organization-and-transcription-factor-families
#19
Tal Golan-Lagziel, Yair E Lewis, Omer Shkedi, Guy Douvdevany, Lilac H Caspi, Izhak Kehat
Cardiac fibroblasts play key roles in both health and disease. Their regulatory elements, transcription factors (TFs), and mechanisms of expression control have not been fully elucidated. We used a differential open chromatin approach, coupled with active enhancer mark, transcriptomic, and computational TFs binding analysis to map cell-type-specific active enhancers in cardiac fibroblasts and cardiomyocytes, and outline the TFs families that control them. This approach was validated by its ability to uncover the known cardiomyocyte TF biology in an unbiased manner, and was then applied to cardiac fibroblasts...
February 5, 2018: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/29420788/phage-display-peptide-libraries-deviations-from-randomness-and-correctives
#20
Arie Ryvkin, Haim Ashkenazy, Yael Weiss-Ottolenghi, Chen Piller, Tal Pupko, Jonathan M Gershoni
Peptide-expressing phage display libraries are widely used for the interrogation of antibodies. Affinity selected peptides are then analyzed to discover epitope mimetics, or are subjected to computational algorithms for epitope prediction. A critical assumption for these applications is the random representation of amino acids in the initial naïve peptide library. In a previous study, we implemented next generation sequencing to evaluate a naïve library and discovered severe deviations from randomness in UAG codon over-representation as well as in high G phosphoramidite abundance causing amino acid distribution biases...
February 6, 2018: Nucleic Acids Research
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