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https://www.readbyqxmd.com/read/28927238/factor-v-leiden-is-associated-with-increased-sperm-count
#1
T E van Mens, U N Joensen, Z Bochdanovits, A Takizawa, J Peter, N Jørgensen, P B Szecsi, J C M Meijers, H Weiler, E Rajpert-De Meyts, S Repping, S Middeldorp
STUDY QUESTION: Is the thrombophilia mutation factor V Leiden (FVL) associated with an increased total sperm count? SUMMARY ANSWER: Carriers of FVL have a higher total sperm count than non-FVL-carriers, which could not be explained by genetic linkage or by observations in a FVL-mouse model. WHAT IS KNOWN ALREADY: FVL has a high prevalence in Caucasians despite detrimental health effects. Carriers have been shown to have higher fecundity, which might partly explain this evolutionary paradox...
September 12, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28926871/multiple-organic-cation-transporters-contribute-to-the-renal-transport-of-sulpiride
#2
Liping Li, Yayun Weng, Wei Wang, Mengru Bai, Hongmei Lei, Hui Zhou, Huidi Jiang
Sulpiride, a selective dopamine D2 receptor blocker, is widely used for treatment of schizophrenia, depression, and gastric/duodenal ulcers. Because of the great majority of sulpiride in positive charged at physiological pH7.4, and ~70% of dose recovered in urine in unchanged form after human intravenous administration of sulpiride, we believe transporters play an important role in renal excretion of sulpiride. The aim of the present study was to explore which transporters contribute to the renal disposition of sulpiride...
September 19, 2017: Biopharmaceutics & Drug Disposition
https://www.readbyqxmd.com/read/28926819/use-of-electro-olfactography-to-measure-olfactory-acuity-in-the-north-american-bullfrog-lithobates-rana-catesbeiana-tadpole
#3
Jody L Heerema, Caren C Helbing, Greg G Pyle
Olfaction is an important sense for aquatic organisms because it provides information about their surroundings, including nearby food, mates, and predators. Electro-olfactography (EOG) is an electrophysiological technique that measures the response of olfactory tissue to olfactory stimuli, and responses are indicative of olfactory acuity. Previous studies have used this technique on a variety of species including frogs, salamanders, daphniids and, most extensively, fish. In the present study, we introduce a novel modified EOG method for use on Lithobates (Rana) catesbeiana tadpoles...
September 16, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28926153/comparative-and-population-genomics-landscape-of-phellinus-noxius-a-hypervariable-fungus-causing-root-rot-in-trees
#4
Chia-Lin Chung, Tracy J Lee, Mitsuteru Akiba, Hsin-Han Lee, Tzu-Hao Kuo, Dang Liu, Huei-Mien Ke, Toshiro Yokoi, Marylette B Roa, Meiyeh J Lu, Ya-Yun Chang, Pao-Jen Ann, Jyh-Nong Tsai, Chien-Yu Chen, Shean-Shong Tzean, Yuko Ota, Tsutomu Hattori, Norio Sahashi, Ruey-Fen Liou, Taisei Kikuchi, Isheng J Tsai
The order Hymenochaetales of white rot fungi contain some of the most aggressive wood decayers causing tree deaths around the world. Despite their ecological importance and the impact of diseases they cause, little is known about the evolution and transmission patterns of these pathogens. Here, we sequenced and undertook comparative genomics analyses of Hymenochaetales genomes using brown root rot fungus Phellinus noxius, wood-decomposing fungus Phellinus lamaensis, laminated root rot fungus Phellinus sulphurascens, and trunk pathogen Porodaedalea pini...
September 19, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28925526/sub1-has-photoreceptor-dependent-and-independent-functions-in-sexual-development-and-secondary-metabolism-in-trichoderma-reesei
#5
Hoda Bazafkan, Sabrina Beier, Eva Stappler, Stefan Böhmdorfer, Josua T Oberlerchner, Michael Sulyok, Monika Schmoll
Light dependent processes are involved in the regulation of growth, development and enzyme production in Trichoderma reesei. The photoreceptors BLR1, BLR2 and ENV1 exert crucial functions in these processes. We analyzed the involvement of the transcription factor SUB1 in sexual development as well as secondary metabolism and its position in the light signaling cascade. SUB1 influences growth and in contrast to its homologue in N. crassa, SUB1 is not essential for fruiting body formation and male fertility in T...
September 19, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28925517/differences-in-protein-expression-among-five-species-of-stream-stonefly-plecoptera-along-a-latitudinal-gradient-in-japan
#6
Maribet Gamboa, Maria Claret Tsuchiya, Suguru Matsumoto, Hisato Iwata, Kozo Watanabe
Proteome variation among natural populations along an environmental gradient may provide insights into how the biological functions of species are related to their local adaptation. We investigated protein expression in five stream stonefly species from four geographic regions along a latitudinal gradient in Japan with varying climatic conditions. The extracted proteins were separated by two-dimensional gel electrophoresis and identified by matrix-assisted laser desorption/ionization of time-of-flight (MALDI TOF/TOF), yielding 446 proteins...
September 19, 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/28925477/the-study-on-the-effect-of-hyperglycemia-on-offspring-fatty-tissue-metabolism-during-pregnancy
#7
H-P Dong, M-Z Tan, Q-J Liu, J Wang, S-B Zhong
OBJECTIVE: In this study, we adopted maternal diabetes model on rats, which induced by streptozotocin to explore the metabolism changes of rat adipose tissue during the neonatal period. MATERIALS AND METHODS: The female rats were induced as diabetes models by streptozotocin (STZ), and mated with the normal male rats when they entered into adulthood. The chosen male offspring rats were executed at week 12 and the epididymis and subcutaneous fat pad were obtained...
August 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28924516/characterization-and-transferability-of-microsatellites-for-the-kangaroo-paw-anigozanthos-manglesii-haemodoraceae
#8
Bronwyn M Ayre, Janet M Anthony, David G Roberts, Richard J N Allcock, Siegfried L Krauss
PREMISE OF THE STUDY: Microsatellites were developed for the future assessment of population genetic structure, mating system, and dispersal of the perennial kangaroo paw, Anigozanthos manglesii (Haemodoraceae), and related species. METHODS AND RESULTS: Using a Personal Genome Machine (PGM) semiconductor sequencer, ca. 4.03 million sequence reads were generated. QDD pipeline software was used to identify 190,000 microsatellite-containing regions and priming sites...
August 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28924513/identification-and-characterization-of-microsatellite-loci-in-the-tuliptree-liriodendron-tulipifera-magnoliaceae
#9
Ricardo Gutiérrez-Ozuna, Matthew B Hamilton
PREMISE OF THE STUDY: Twenty-three polymorphic microsatellite loci (simple sequence repeats) were identified and characterized for Liriodendron tulipifera (Magnoliaceae), a species native to eastern North America, to investigate its genetic diversity, population structure, and mating system. METHODS AND RESULTS: Using Illumina HiSeq paired-end reads from genomic DNA, searches for repeat motifs identified approximately 280,000 potentially amplifiable loci. Of 77 loci tested, 51 amplified consistently...
August 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28924494/genomic-and-functional-analysis-of-romboutsia-ilealis-crib-t-reveals-adaptation-to-the-small-intestine
#10
Jacoline Gerritsen, Bastian Hornung, Bernadette Renckens, Sacha A F T van Hijum, Vitor A P Martins Dos Santos, Ger T Rijkers, Peter J Schaap, Willem M de Vos, Hauke Smidt
BACKGROUND: The microbiota in the small intestine relies on their capacity to rapidly import and ferment available carbohydrates to survive in a complex and highly competitive ecosystem. Understanding how these communities function requires elucidating the role of its key players, the interactions among them and with their environment/host. METHODS: The genome of the gut bacterium Romboutsia ilealis CRIB(T) was sequenced with multiple technologies (Illumina paired-end, mate-pair and PacBio)...
2017: PeerJ
https://www.readbyqxmd.com/read/28923935/retinoid-x-receptor-suppresses-a-metastasis-promoting-transcriptional-program-in-myeloid-cells-via-a-ligand-insensitive-mechanism
#11
Mate Kiss, Zsolt Czimmerer, Gergely Nagy, Pawel Bieniasz-Krzywiec, Manuel Ehling, Attila Pap, Szilard Poliska, Pal Boto, Petros Tzerpos, Attila Horvath, Zsuzsanna Kolostyak, Bence Daniel, Istvan Szatmari, Massimiliano Mazzone, Laszlo Nagy
Retinoid X receptor (RXR) regulates several key functions in myeloid cells, including inflammatory responses, phagocytosis, chemokine secretion, and proangiogenic activity. Its importance, however, in tumor-associated myeloid cells is unknown. In this study, we demonstrate that deletion of RXR in myeloid cells enhances lung metastasis formation while not affecting primary tumor growth. We show that RXR deficiency leads to transcriptomic changes in the lung myeloid compartment characterized by increased expression of prometastatic genes, including important determinants of premetastatic niche formation...
September 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28923618/impact-of-induction-therapy-on-delayed-graft-function-following-kidney-transplantation-in-mated-kidneys
#12
T Butler, N Hayde
BACKGROUND: Delayed graft function (DGF) is defined as the need for dialysis within 1 week of transplantation and occurs in 20%-50% of deceased-donor kidney transplant recipients. Although recovery from DGF often occurs within a few days, many cases may take weeks to months before the transplant function begins. The delay in function increases the complexity of recipient care, makes the diagnosis of acute rejection more difficult, prolongs length of stay, and increases hospital costs...
October 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28923316/different-combinations-of-maternal-and-postnatal-diet-are-reflected-in-changes-of-hepatic-parenchyma-and-hepatic-tnf-alpha-expression-in-male-rat-offspring
#13
Željka Perić Kačarević, Anđela Grgić, Darija Šnajder, Nikola Bijelić, Tatjana Belovari, Olga Cvijanović, Valerija Blažičević, Radivoje Radić
Obesity is related to increased TNF-alpha production in different tissues. TNF-alpha is connected to mitochondrial dysfunction in the liver and also development of fatty infiltration of the liver. Also, postnatal change from normal to high-fat diet causes a significant increase in TNF-alpha serum levels. The aim of this research was to determine how maternal diet and switching male offspring to a different dietary regime after lactation influences rat liver. Ten female Sprague Dawley rats at nine weeks of age were randomly divided in two groups and fed either standard laboratory chow or high-fat diet during six weeks, and then mated with the same male subject...
September 15, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28921999/predictive-validity-and-adjustment-of-ideal-partner-preferences-across-the-transition-into-romantic-relationships
#14
Tanja M Gerlach, Ruben C Arslan, Thomas Schultze, Selina K Reinhard, Lars Penke
Although empirical research has investigated what we ideally seek in a romantic partner for decades, the crucial question of whether ideal partner preferences actually guide our mating decisions in real life has remained largely unanswered. One reason for this is the lack of designs that assess individuals' ideal partner preferences before entering a relationship and then follow up on them over an extended period. In the Göttingen Mate Choice Study (GMCS), a preregistered, large-scale online study, we used such a naturalistic prospective design...
September 18, 2017: Journal of Personality and Social Psychology
https://www.readbyqxmd.com/read/28921898/maternal-chronic-folate-supplementation-ameliorates-behavior-disorders-induced-by-prenatal-high-fat-diet-through-methylation-alteration-of-bdnf-and-grin2b-in-offspring-hippocampus
#15
Zhonghai Yan, Fei Jiao, Xiaoshuang Yan, Hailong Ou
SCOPE: Maternal consumption of a high-fat diet (HFD) during pregnancy increases the risk of behavioral problems. Folate plays an important role in neuroplasticity and the preservation of neuronal integrity. This study aimed at determining the influence of diets supplemented with folate on offspring behavior, and the mechanisms involved. METHODS AND RESULTS: Female mice were fed a control diet, a high-fat diet, control diet supplemented with folate, or a high-fat diet supplemented with folate for 5 wks before mating...
September 17, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28921784/the-evolution-of-gonad-expenditure-and-gonadosomatic-index-gsi-in-male-and-female-broadcast-spawning-invertebrates
#16
Geoff A Parker, Steven A Ramm, Jussi Lehtonen, Jonathan M Henshaw
Sedentary broadcast-spawning marine invertebrates, which release both eggs and sperm into the water for fertilization, are of special interest for sexual selection studies. They provide unique insight into the early stages of the evolutionary succession leading to the often-intense operation of both pre- and post-mating sexual selection in mobile gonochorists. Since they are sessile or only weakly mobile, adults can interact only to a limited extent with other adults and with their own fertilized offspring...
September 18, 2017: Biological Reviews of the Cambridge Philosophical Society
https://www.readbyqxmd.com/read/28921699/morphological-determinants-of-carrier-frequency-signal-in-katydids-orthoptera-a-comparative-analysis-using-biophysical-evidence
#17
Fernando Montealegre-Z, Jessica Ogden, Thorin Jonsson, Carl D Soulsbury
Male katydids produce mating calls by stridulation using specialized structures on the forewings. The right wing (RW) bears a scraper connected to a drum-like cell known as the mirror and a left wing (LW) that overlaps the RW and bears a serrated vein on the ventral side, the stridulatory file. Sound is generated with the scraper sweeping across the file, producing vibrations that are amplified by the mirror. Using this sound generator, katydids exploit a range of song carrier frequencies (CF) unsurpassed by any other insect group, with species singing as low as 600 Hz and others as high as 150 kHz...
September 16, 2017: Journal of Evolutionary Biology
https://www.readbyqxmd.com/read/28921050/characterization-of-ccstop1-a-c2h2-type-transcription-factor-regulates-al-tolerance-gene-in-pigeonpea
#18
Abhijit Arun Daspute, Yuriko Kobayashi, Sanjib Kumar Panda, Bashasab Fakrudin, Yasufumi Kobayashi, Mutsutomo Tokizawa, Satoshi Iuchi, Arbind Kumar Choudhary, Yoshiharu Y Yamamoto, Hiroyuki Koyama
Al-responsive citrate-transporting CcMATE1 function and its regulation by CcSTOP1 were analyzed using NtSTOP1 -KD tobacco- and pigeonpea hairy roots, respectively, CcSTOP1 binding sequence of CcMATE1 showed similarity with AtALMT1 promoter. The molecular mechanisms of Aluminum (Al) tolerance in pigeonpea (Cajanus cajan) were characterized to provide information for molecular breeding. Al-inducible citrate excretion was associated with the expression of MULTIDRUGS AND TOXIC COMPOUNDS EXCLUSION (CcMATE1), which encodes a citrate transporter...
September 18, 2017: Planta
https://www.readbyqxmd.com/read/28920934/esr1-cells-in-the-ventromedial-hypothalamus-control-female-aggression
#19
Koichi Hashikawa, Yoshiko Hashikawa, Robin Tremblay, Jiaxing Zhang, James E Feng, Alexander Sabol, Walter T Piper, Hyosang Lee, Bernardo Rudy, Dayu Lin
As an essential means of resolving conflicts, aggression is expressed by both sexes but often at a higher level in males than in females. Recent studies suggest that cells in the ventrolateral part of the ventromedial hypothalamus (VMHvl) that express estrogen receptor-α (Esr1) and progesterone receptor are essential for male but not female mouse aggression. In contrast, here we show that VMHvl(Esr1+) cells are indispensable for female aggression. This population was active when females attacked naturally...
September 18, 2017: Nature Neuroscience
https://www.readbyqxmd.com/read/28920796/successful-laparoscopic-oviductal-artificial-insemination-in-the-clouded-leopard-neofelis-nebulosa-in-thailand
#20
Wanlaya Tipkantha, Paweena Thuwanut, Umaporn Maikeaw, Ampika Thongphakdee, Saifon Yapila, Sumate Kamolnorranath, Boripat Siriaroonrat, Pierre Comizzoli, Kaywalee Chatdarong
Captive breeding of clouded leopards (Neofelis nebulosa) is challenging because of mating incompatibility, high incidence of teratospermia in males, and inconsistent ovulation patterns in females. Assisted reproductive techniques, therefore, are necessary to overcome these issues and maintain the genetic diversity in the captive population. The objective was to use laparoscopic oviductal artificial insemination (AI) to breed genetically valuable females (n = 4; aged 4.5-5 yr) that were unsuccessfully paired...
September 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
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