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Newcastle disease virus

Xiaojing Xu, Qing Sun, Yu Mei, Yonghao Liu, Lixiang Zhao
IL15 and IL7 are two cytokines essential for T cell development and homeostasis. In order to improve the antitumor activity by Newcastle Disease Virus (NDV)-modified tumor vaccine, we generated a recombinant NDV co-expressing IL15 and IL7 (LX/IL(15+7)) by incorporation of a 2A self-processing peptide into IL15 and IL7 using reverse genetics. B16 cells infected with LX/IL(15+7) expressed both IL15 and IL7 stably. The cytotoxicity assay showed that murine melanoma cells modified with LX/IL(15+7) could significantly enhance the antitumor immune response in vitro...
December 10, 2017: Cancer Science
Nacira Laamiri, Rim Aouini, Boutheina Marnissi, Abdeljelil Ghram, Issam Hmila
A one-step multiplex real-time reverse transcription-PCR (rRT-PCR) assay was developed for simultaneous detection and quantification of four avian respiratory viruses: avian influenza virus (AIV), infectious bronchitis virus (IBV), Newcastle disease virus (NDV) and infectious laryngotracheitis virus (ILTV). In comparison with the singleplex rRT-PCR, the specificity, the sensitivity and the reproducibility of the new assay were evaluated and validated using 70 clinical samples. The optimal cutoff point, the corresponding limit of quantification (LoQ) and the limit of detection (LoD) were statistical established based on receiver operating characteristic (ROC) curve analysis...
December 7, 2017: Virology
Yongxia Guo, Qiang Zhang, Zonghui Zuo, Jun Chu, Hongzhi Xiao, M Tariq Javed, Cheng He
Protocatechuic acid (PCA) is an antiviral agent against Avian Influenza virus (AIV) and Infectious Bursal Disease (IBD) virus but its antiviral mechanism is unknown. In this study, we evaluated the humoral and cellular responses to PCA in specific pathogen-free (SPF) chickens. One hundred forty 35-day-old SPF chickens were randomly divided into 7 groups. The birds were inoculated with the commercial, attenuated Newcastle Disease Virus (NDV) vaccine and then received orally with 10, 20 or 40 mg/kg body weight of PCA for 21 days...
December 4, 2017: Microbial Pathogenesis
J C S Martinez, W K Chou, L R Berghman, J B Carey
Newcastle disease remains a major concern to the poultry industry; however, it can be managed with effective vaccination programs. The main objective of this study was to evaluate the effect of 2 1× doses of live LaSota strain Newcastle disease virus (NDV) vaccine administered oculo-nasally on d one and 21 on development of humoral and cell-mediated immune response in broilers, and to compare different immunization schedules. Two experiments were conducted. In Experiment I (n = 320), Ross 308 birds were randomly assigned to an unvaccinated control group or vaccinated treatment...
December 2, 2017: Poultry Science
Loveline N Ngu, Nadesh N Nji, Georgia Ambada, Apeh A Ngoh, Ghislain D Njambe Priso, Jules C Tchadji, Abel Lissom, Suzanne H Magagoum, Carol N Sake, Thibau F Tchouangueu, George O Chukwuma, Arinze S Okoli, Bertrand Sagnia, Rebecca Chukwuanukwu, Denis M Tebit, Charles O Esimone, Alain B Waffo, Chae G Park, Klaus Überla, Godwin W Nchinda
INTRODUCTION: Recombinant Newcastle Disease virus (rNDV) vectored vaccines are safe mucosal applicable vaccines with intrinsic immune-modulatory properties for the induction of efficient immunity. Like all viral vectored vaccines repeated inoculation via mucosal routes invariably results to immunity against viral vaccine vectors. To obviate immunity against viral vaccine vectors and improve the ability of rNDV vectored vaccines in inducing T cell immunity in murine air way we have directed dendritic cell targeted HIV-1 gag protein (DEC-Gag) vaccine; for the induction of helper CD4+ T cells to a Recombinant Newcastle disease virus expressing codon optimized HIV-1 Gag P55 (rNDV-L-Gag) vaccine...
December 4, 2017: Immunity, Inflammation and Disease
Hongjing Zhao, Yu Wang, Yizhi Shao, Juanjuan Liu, Jinglun Li, Hui Zong, Mingwei Xing
Whopper swan Cygnus cygnus interferon alpha (CyIFN-α) gene was amplified from genomic DNA in the liver of whooper swan, which encodes a 192-amino acid protein containing a 29-amino acid signal peptide. Bioinformatics analysis showed it containes 7 cysteine residues and 5 alpha helices. After expression in prokaryote, purification and renaturation, CyIFN-α was analyzed on its physicochemical property and antiviral activity. In chicken embryo fibroblasts, CyIFN-α exerted more superior anti-vesicular stomatitis virus (VSV), avian influenza virus (AIV), and Newcastle disease virus (NDV) activity than chicken IFN-α, an effective therapeutic agent used for viral infections clinically...
November 30, 2017: Journal of Interferon & Cytokine Research
V Mayahi, M Esmaelizad
Hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) plays a crucial role in induction of immune response against Newcastle disease infection. Mutation in residues 347 and 362 of HN linear antigenic site has been identified to be responsible for antigenic variations. Hence we studied antigenic difference between sub-genotype VIIj isolates and vaccine strains by the use of polyclonal serum against LaSota strain in hemagglutination inhibition test. Furthermore, epitope patterns of the isolates under study were analyzed and compared to HN sequences in GenBank...
2017: Acta Virologica
Zeinab Mohamed Ali, Mervat Abd El Monaem Hassan, Hussein Ali Hussein, Basem Mohamed Ahmed, Ahmed Abd El-Ghany El Sanousi
Aim: The objective of the present study was to prepare a trivalent inactivated vaccine of Newcastle disease virus (NDV), H5N1, and H9N2 viruses. Materials and Methods: Three monovalent and a trivalent vaccines were prepared by emulsifying inactivated NDV (LaSota strain), reassortant H5N1, and H9N2 viruses with Montanide ISA 71 oil adjuvant. Parameters used for evaluation of the efficacy of the prepared vaccines in specific pathogen-free chickens were cellular immunity assays (blastogenesis, interferon gamma, interleukin 1 [IL1], and IL6), humoral immunity by hemagglutination inhibition, protection percentage, and shedding...
October 2017: Veterinary World
Haider Kaab, Maureen M Bain, Peter David Eckersall
Vaccination is an important tool in poultry health, but is itself a stressor often resulting in a reduction in feed intake, body weight gain, and nutrient digestibility. In other species, vaccination is associated with an immediate acute-phase response. As an important immune parameter, the circulating heterophil/lymphocyte (H/L) ratio is a well-recognized parameter of stress in poultry. In this study, the effects of a routinely used commercial poultry vaccine on the acute phase response (APR) and H/L ratios in specific pathogen-free (SPF) layer chicks was examined to determine if post vaccination (PV) stress and an APR occur...
November 22, 2017: Poultry Science
Kai Zhao, Jinyu Han, Yang Zhang, Lin Wei, Shuang Yu, Xiaohua Wang, Zheng Jin, Yun-Feng Wang
Because mucosal sites are the entry ports of pathogens, immunization via mucosal routes can extremely enhance the immunity. To elevate the potential of N-2-hydroxypropyl trimethyl ammonium chloride chitosan (N-2-HACC) and N,O-carboxymethyl chitosan (CMC) nanoparticles as a mucosal immune delivery carrier for DNA vaccines, we prepared the NDV F gene plasmid DNA with C3d6 molecular adjuvant (pVAX I -F(o)-C3d6) encapsulated in the N-2-HACC-CMC nanoparticles (N-2-HACC-CMC/pFDNA-C3d6 NPs). The N-2-HACC-CMC/pFDNA-C3d6 NPs had regular spherical morphology and low toxicity with a mean diameter of 309...
November 27, 2017: Molecular Pharmaceutics
Ana Chumbe, Ray Izquierdo-Lara, Katherine Calderón, Manolo Fernández-Díaz, Vikram N Vakharia
BACKGROUND: Newcastle disease is one of the most important infectious diseases of poultry, caused by Newcastle disease virus (NDV). This virus is distributed worldwide and it can cause severe economic losses in the poultry industry due to recurring outbreaks in vaccinated and unvaccinated flocks. Protection against NDV in chickens has been associated with development of humoral response. Although hemagglutination inhibition (HI) assay and ELISA do not corroborate the presence of neutralizing antibodies (nAbs); they are used to measure protection and immune response against NDV...
November 23, 2017: Virology Journal
Camile Lugarini, Renata Hurtado, Jansen de Araujo, Tatiana Ometto, Luciano Thomazelli, Marina de Seixas, Edison Durigon, Jean Carlos Silva
We tested 529 wild birds captured in northeastern Brazil for infection by avian influenza, Newcastle disease, and West Nile. Viruses were not detected by real-time PCR with the exception of one Tropical Gnatcatcher (Polioptila plumbea) positive for influenza virus, but this could not be confirmed by viral isolation or gene sequencing.
November 17, 2017: Journal of Wildlife Diseases
Vienna R Brown, Sarah N Bevins
After publication of the article [1], it has been brought to our attention that Newcastle disease virus was incorrectly labeled as a Tier 1 USDA Select Agent. Newcastle disease virus is a USDA Select Agent but it is not a Tier 1 agent.
November 16, 2017: Veterinary Research
X Niu, H Wang, L Wei, M Zhang, J Yang, H Chen, Y Tang, Y Diao
To deepen the knowledge about epidemic prevalence in the goose breeding field, a triplex PCR assay was established, and 478 samples were collected from scaled goose farms in 11 provinces in China. The results of this epidemiological investigation showed that incidence rates of H9 avian influenza and goose circovirus were the highest among five infectious diseases that were evaluated. In addition, the triplex PCR assay established remarkable sensitivity, rapidity and versatility compared to other diagnostic methods...
November 13, 2017: Transboundary and Emerging Diseases
S H Andersen, L Vervelde, K Sutton, L R Norup, E Wattrang, H R Juul-Madsen, T S Dalgaard
The aim of this study was to optimise and evaluate an intracellular cytokine staining (ICS) assay for assessment of T cell IFN-γ responses in chickens vaccinated against Newcastle disease (ND). We aimed to validate currently available antibodies to chicken IFN-γ using transfected CHO cells. Moreover, this ICS assay was evaluated for use to detect mitogen and antigen induced IFN-γ production in chicken peripheral blood leucocytes. Chickens from an inbred white leghorn line containing two MHC haplotypes, B19 and B21, were divided into three experimental groups; one group was kept as naive controls, one group was vaccinated intramuscularly twice with a commercial inactivated ND virus (NDV) vaccine, and the last group was vaccinated orally twice with a commercial live attenuated NDV vaccine...
December 2017: Veterinary Immunology and Immunopathology
Yongzhong Cao, Qian Liu, Xiaorong Zhang, Hai Hu, Yantao Wu
In this study, the complete genome sequence of the heat-resistant Newcastle disease virus (NDV) strain HR09, which was isolated in China in the 1990s, was determined and characterized phylogenetically. This is the first report regarding the complete genomic information of a thermostable NDV strain from China.
November 2, 2017: Genome Announcements
Vienna R Brown, Sarah N Bevins
Newcastle disease is caused by virulent strains of Newcastle disease virus (NDV), which causes substantial morbidity and mortality events worldwide in poultry. The virus strains can be differentiated as lentogenic, mesogenic, or velogenic based on a mean death time in chicken embryos. Currently, velogenic strains of NDV are not endemic in United States domestic poultry; however, these strains are present in other countries and are occasionally detected in wild birds in the U.S. A viral introduction into domestic poultry could have severe economic consequences due to the loss of production from sick and dying birds, the cost of control measures such as depopulation and disinfection measures, and the trade restrictions that would likely be imposed as a result of an outbreak...
October 26, 2017: Veterinary Research
Etriwati, Dewi Ratih, Ekowati Handharyani, Surachmi Setiyaningsih
AIM: The aim of the study was to examine pathology and the distribution pattern of Newcastle disease virus (NDV) in internal organs of chickens from a field case using immunohistochemical staining. MATERIALS AND METHODS: 10 groups of broiler, layer, and domestic chicken were collected from necropsy room Division of Pathology, Bogor Agricultural University. These chickens were originated from West Java and collected based on pathologist diagnosis as suspect of Newcastle disease (ND)...
September 2017: Veterinary World
Wenliang Li, Li Mao, Yongguo Cao, Bin Zhou, Leilei Yang, Linxiao Han, Fei Hao, Tao Lin, Wenwen Zhang, Jieyuan Jiang
BACKGROUND: Classical swine fever virus (CSFV) is the causative pathogen of Classical swine fever (CSF), a highly contagious disease of swine. Viperin is one of the hundreds of interferon-stimulated genes (ISGs), and possesses a wide range of antiviral activities. The aim of this study was to explore whether porcine Viperin has the anti-CSFV activity. METHOD: The influences of CSFV infection on Viperin expression and Newcastle disease virus (NDV)/Pseudorabies virus (PRV)-induced Viperin expression were examined in 3D4/21 cells and porcine peripheral blood mononuclear cells (PBMCs)...
October 23, 2017: Virology Journal
Kai Zhao, Xutong Duan, Lianwei Hao, Xiaohua Wang, Yunfeng Wang
Newcastle disease is a serious infectious disease in the poultry industry. The commercial vaccines can only offer limited protection and some of them are expensive and need adjuvants. At present, DNA vaccines are widely used. However, the immune responses induced by DNA vaccines are too slow and low. Here, we constructed the transfer vectors with a different number of C3d as molecular adjuvants (n = 1, 2, 4, or 6), and the vectors were cloned into the optimal eukaryotic expression plasmid (pVAXI-optiF) that expressed the F gene of Newcastle disease virus (NDV), and named pVAXI-F(o)-C3d1, pVAXI -F(o)-C3d2, pVAXI-F(o)-C3d4, and pVAXI-F(o)-C3d6, respectively...
November 28, 2017: Journal of Microbiology and Biotechnology
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