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Newcastle disease virus

Bin Xiang, Lujie Han, Pei Gao, Renrong You, Fumin Wang, Jiajie Xiao, Ming Liao, Yinfeng Kang, Tao Ren
Despite intensive vaccination programs in many countries, including China, Newcastle disease has been reported sporadically and is still a significant threat to the poultry industry in China. Newcastle disease virus (NDV) is infectious for at least 250 bird species, but the role of wild birds in virus epidemiology remains largely unknown. Fourteen NDV isolates were obtained from 2040 samples collected from wild birds or the environment in Guangdong province, southern China, from 2013 to 2015. The isolation rate was the highest in the period of wintering and lowest during the periods of spring migration, nesting, and postnesting...
September 18, 2017: Infection, Genetics and Evolution
Raghunath Shobana, Raghavendra Sumanth Pudupakam, Adria Allen, Moanaro Biswas, Nammalwar Sriranganathan
Oncolytic virotherapy is a promising novel approach that overcomes the limitations posed by radiation and chemotherapy. In this study, the oncolytic efficacy of a recombinant Newcastle disease virus (rNDV) BC-KLQL-GFP, against prostate cancer stem-like/tumor initiating cells was evaluated. Xenograft derived prostaspheres (XPS) induced tumor more efficiently than monolayer cell derived prostaspheres (MCPS) in nude mice. Primary and secondary XPS show enhanced self-renewal and clonogenic potential compared to MCPS...
September 18, 2017: Journal of Biotechnology
Binbin Luo, Yangfei Xu, Shengxi Wu, Mingfu Zhao, Pengjun Jiang, Shenghui Shi, Zhonghao Zhang, Yin Wang, Linlin Wang, Yong Liu
A novel immunosensor for detecting Newcastle disease virus (NDV) was developed using excessively tilted fiber grating (Ex-TFG) coated with gold nanospheres (AuNs). AuNs were coated on the Ex-TFG surface via Au-S bonds using 3-mercaptopropyltrimethoxysilane (MPTMS), and the activated staphylococcal protein A (SPA) was linked to AuNs by covalent bonds via cysteamine. AuNs greatly enhanced the impact of the analyte on the fiber cladding mode through the local surface Plasmon resonance (LSPR) effect, thus improving the detection limit and sensitivity of the immunosensor...
September 1, 2017: Biosensors & Bioelectronics
Yusuke Matsumoto, Keisuke Ohta, Machiko Nishio
Leader sequence, located at the 3' terminus of paramyxovirus genomes, determines the degree of viral transcription and replication. The essential nucleotides in the leader sequence that influence viral propagation, however, have not been investigated in detail. In the present study, we show that polymerase complex of human parainfluenza virus type 2 (hPIV2) uses a luciferase-encoding hPIV2 mini-genome possessing the leader sequence from other closely related viruses as a template. Furthermore, we demonstrate that although hPIV2 polymerase complex can recognize the leader sequence of hPIV4B, mumps virus (MuV) and PIV5 as well as Newcastle disease virus (NDV), it cannot recognize measles virus, hPIV1, Sendai virus (SeV) or hPIV3...
September 7, 2017: Medical Microbiology and Immunology
Ruiguang Xu, Jiahua Weng, Liwen Hu, Guangneng Peng, Zhihua Ren, Junliang Deng, Yan Jia, Chengmin Wang, Hongxuan He, Yanchun Hu
The purpose of this study was to investigate the anti-Newcastle disease virus (NDV) activities of 9-oxo-10,11-dehydroageraphorone (euptox A) from Eupatorium adenophorum Spreng (E. adenophorum) in vitro. NDV infection of chicken embryo fibroblasts (CEFs) was performed. Cytotoxicities and antiviral activities of euptox A was assessed by the MTT method. The interaction of NDV with cell membrane protein was detected by virus overlay protein binding assay (VOPBA). The expression levels of NDV genes in CEFs was tested by RTFQ PCR...
September 7, 2017: Natural Product Research
Ji-Hui Jin, Jin-Long Cheng, Zi-Rong He, Ying-Chao Ren, Xiao-Hui Yu, Yang Song, Hui-Ming Yang, Yan-Ling Yang, Tong Liu, Guo-Zhong Zhang
To investigate the exact effects of different origins of Newcastle disease virus (NDV) hemagglutinin-neuraminidase (HN) protein to the biological characteristics of the virus, we systematically studied the correlation between the HN protein and NDV virulence by exchanging the HN of velogenic or lentogenic NDV strains with the HN from other strains of different virulence. The results revealed that the rSG10 or rLaSota derivatives bearing the HN gene of other viruses exhibited decreased or increased hemadsorption (HAd), neuraminidase and fusion promotion activities...
2017: Frontiers in Microbiology
Fereshteh Sabouri, Mehdi Vasfi Marandi, Mohsen Bashashati
Newcastle disease (ND) is an economically important and highly contagious disease of wild and domestic avian species. Despite extensive vaccination efforts of industrial poultry, Newcastle disease virus (NDV) outbreaks causing significant economic losses still occur. Rural chickens may act as a potential reservoir of NDVs for commercial poultry due to poor biosecurity and inadequate vaccination. The aim of this study was to investigate phylogenetic relationship and molecular characterization of eight NDVs isolated from backyard poultry during 2011-2013...
September 6, 2017: Avian Pathology: Journal of the W.V.P.A
Haijin Liu, Renata Servan de Almeida, Patricia Gil, Emmanuel Albina
Rescue of negative-sense single-stranded RNA viruses ((-)ssRNA virus), generally requires the handling of a large number of plasmids to provide the virus genome and essential components for gene expression and genome replication. This constraint probably renders reverse genetics of (-)ssRNA virus more complex and less efficient. Some authors have shown that the fewer the plasmids, the more efficient reverse genetics is for segmented RNA virus. However, it is not clear if the same applies for (-)ssRNA, such as Newcastle disease virus (NDV)...
September 1, 2017: Journal of Virological Methods
Mai Takamura-Ishii, Takahiro Miura, Takaaki Nakaya, Katsuro Hagiwara
Fibrosarcoma is a locally aggressive malignant tumor with a high recurrence rate, so that wide excisional surgery is necessary for treatment. However, it is often difficult to resect with a sufficient margin of excision at the site of tumor infiltration. Recombinant tumor vaccine therapy is a useful method to induce specific immunity. In this study, we have shown its utility as a candidate for therapy by applying a recombinant Newcastle disease virus (rNDV) tumor vaccine (rNDV-TV). Although the therapeutic effect of similar viruses has been examined in several tumors, the vaccination efficacy against fibrosarcoma has not been demonstrated until now...
September 1, 2017: Medical Oncology
Vafa Mayahi, Majid Esmaelizad
In the case of Newcastle disease virus, multiple factors such as host adaptation, immune response evasion, and selective pressures have been suggested to result in evolution of viruses and the emergence of genetic variants. Multiple studies on virus classification and global epidemiological links have yielded consistent data. Here, we have performed a molecular analysis study of circulating Newcastle disease viruses in Iran (1995-2016). According to evolutionary divergences, subgenotype VIg, VIj, VIIj, VIId, XIIIa and XIIId isolates have been circulating in the country during a 21-year period...
September 1, 2017: Archives of Virology
Yanhong Wang, Wanqi Yu, Na Huo, Wenbin Wang, Yuanyuan Guo, Qiaolin Wei, Xinglong Wang, Shuxia Zhang, Zengqi Yang, Sa Xiao
Newcastle disease virus (NDV) is a contagious agent of Newcastle disease in avian species and seriously affects the poultry industry. The cleavage site of the viral F protein (Fcs) is a key determinant of membrane fusion and viral virulence. In this study, we investigated the precise effect of variable amino acid sequences of the Fcs on fusogenic activity. Based on viral pathogenicity, the Fcs sequences of natural isolates (n = 1572) are classified into eight types of virulent Fcs (VFcs) with the motif "G/R/K-R-Q/R/K-R/K-R↓F" and ten types of the avirulent Fcs (AFcs) with the motif "G/R/E-R/K/Q-Q-G/E-R↓L"...
2017: PloS One
Theresa Schwaiger, Michael R Knittler, Christian Grund, Angela Roemer-Oberdoerfer, Joachim-Friedrich Kapp, Markus M Lerch, Thomas C Mettenleiter, Julia Mayerle, Ulrike Blohm
Pancreatic cancer is the 8(th) most common cause of cancer-related deaths worldwide and the tumor with the poorest prognosis of all solid malignancies. In 1957 it was discovered that Newcastle disease virus (NDV) has oncolytic properties on tumor cells. To study the oncolytic properties of NDV in pancreatic cancer a single dose was administered intravenously in a syngenic orthotopic tumor model employing two different murine pancreatic adenocarcinoma cell lines (DT6606PDA, Panc02). Tumor growth was monitored and immune response was analyzed...
August 31, 2017: International Journal of Cancer. Journal International du Cancer
Wei Zhang, Lidan Hou, Jie Song, Shuang Zhang, Yun Li, Jing Li, Lei Sun, Wenhui Fan, Wenjun Liu
H9 subtype avian influenza virus causes worldwide epidemic, resulting in enormous economic losses of poultry production. In the present study, an indirect ELISA method was established for more accurate and specific detection. The recombinant protein of the globular head domain of HA of H9 subtype avian influenza virus was used as antigen. Specific blocking buffers and dilution buffers were determined to increase the sensitivity and specificity. The sensitivity of ELISA was higher than that of hemagglutination inhibition (HI) test...
August 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
Na Zhang, Hongjun Huang, Binghe Tan, Yinglei Wei, Qingqing Xiong, Yan Yan, Lili Hou, Nannan Wu, Stefan Siwko, Andrea Cimarelli, Jianrong Xu, Honghui Han, Min Qian, Mingyao Liu, Bing Du
Vesicular stomatitis virus (VSV) and rabies and Chandipura viruses belong to the Rhabdovirus family. VSV is a common laboratory virus to study viral evolution and host immune responses to viral infection, and recombinant VSV-based vectors have been widely used for viral oncolysis, vaccination, and gene therapy. Although the tropism of VSV is broad, and its envelope glycoprotein G is often used for pseudotyping other viruses, the host cellular components involved in VSV infection remain unclear. Here, we demonstrate that the host protein leucine-rich repeat-containing G protein-coupled receptor 4 (Lgr4) is essential for VSV and VSV-G pseudotyped lentivirus (VSVG-LV) to infect susceptible cells...
August 23, 2017: Journal of Biological Chemistry
Celia Abolnik, Chrisborn Mubamba, George Dautu, Bruce Gummow
The first complete genome sequence of an African-origin Newcastle disease virus belonging to genotype XIII is described here. The virulent strain chicken/Zambia/Chiwoko/2015 was isolated from diseased chickens in 2015.
August 24, 2017: Genome Announcements
Jacob Pitcovski, Eli Pitcovski, Dana Goldenberg, Ehud Shahar
Maternally derived antibodies (MDAs) are critical for offspring protection during the first days of life. However, due to their short half-life (3-5 days), MDA levels decline rapidly and by 8-15 days post-hatch drop to below protective levels. In addition, MDAs against a specific pathogen often impede the efficient protective immune response to that pathogen by the new-born following vaccination. The combination of these two phenomena generates a gap in protection in the period between loss of MDA protection and development of vaccine-induced protective antibodies...
August 22, 2017: Avian Pathology: Journal of the W.V.P.A
Jianchang Wang, Jinfeng Wang, Ruiwen Li, Libing Liu, Wanzhe Yuan
BACKGROUND: Canine distemper, caused by Canine distemper virus (CDV), is a highly contagious and fatal systemic disease in free-living and captive carnivores worldwide. Recombinase polymerase amplification (RPA), as an isothermal gene amplification technique, has been explored for the molecular detection of diverse pathogens. METHODS: A real-time reverse transcription RPA (RT-RPA) assay for the detection of canine distemper virus (CDV) using primers and exo probe targeting the CDV nucleocapsid protein gene was developed...
August 15, 2017: BMC Veterinary Research
Jingjing Liu, Jie Zhu, Haixu Xu, Juan Li, Zenglei Hu, Shunlin Hu, Xiaoquan Wang And, Xiufan Liu
Newcastle disease (ND) leading to heavy economic losses to the poultry industry worldwide is caused by Newcastle disease virus (NDV). Even though intensive vaccination programs have been implemented in many countries, virulent NDV can still be frequently isolated in well-vaccinated flocks. We compared the protection efficiency of LaSota and two sub-genotype VIId vaccines, NDV/AI4 and NDV O/AI4, in which NDV O/AI4 was constructed by replacing the hemagglutinin-neuraminidase (HN) gene of the vaccine strain NDV/AI4 with that from the variant NDV strain JS-14-12-Ch by the cross hemagglutination inhibition test and immune protection test...
August 15, 2017: Viruses
Hongjing Zhao, Yu Wang, Juanjuan Liu, Yizhi Shao, Jinglun Li, Hongliang Chai, Mingwei Xing
DNA and Cell Biology (DNA&CB) is officially retracting the paper by Zhao H, Wang Y, Liu J, Shao Y, Li J, Chai H, Xing M, entitled, "Molecular Characterization and Biological activity of Interferon-α in Indian Peafowl (Pavo cristatus)," [Epub ahead of print]; 2017, DOI: 10.1089/dna.2017.3798. The Editor-in-Chief of DNA&CB, Dr. Carol Shoshkes Reiss, was alerted to a discrepancy between the findings in the article by Zhao et al., and those of others, about the absence of expression of ISG15 in chickens. Dr. Reiss requested from the authors a clarification in their observations and inquired about the failure to include relevant citations in the reference section of the paper...
August 7, 2017: DNA and Cell Biology
Xinglong Wang, Xiangwei Wang, Yanqing Jia, Chongyang Wang, Qinqsong Han, Zen H Lu, Zengqi Yang
Although vaccination has been hugely successful in protecting birds against infection by the New castle disease virus (NDV), newly-emerged highly virulent strains have been found to overcome established immune protection and threaten the poultry industry. The need to improve the immunization efficacy is, therefore, urgent. Here, we tested the potential immunostimulatory adjuvant activity of the adenoviral-expressed recombinant chicken granulocyte monocyte colony stimulating factor (rchGM-CSF) in an inactivated Newcastle Disease Virus (NDV) vaccine...
July 8, 2017: Antiviral Research
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