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Beta cells AND icariin

Shuping Fu, Li Yang, Hao Hong, Ronghua Zhang
OBJECTIVE: To investigate the effect of icariin on proliferation of bone marrow mesenchymal stem cells (BMSCs) in Sprague-Dawley (SD) rats. METHODS: BMSCs were obtained from SD rat bone marrow with differential time adherent method. Its characteristic was identified through differentiation cell surface antigens and the multi-lineage (osteo/adipo/chondo) differentiation potential. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method and 5-Bromo-2-Deoxyuridine (BrdU) incorporation were applied to detect the effect of icariin on BMSCs proliferation...
June 2016: Journal of Traditional Chinese Medicine, Chung i Tsa Chih Ying Wen Pan
Lingwen Kong, Jiaqi Liu, Jia Wang, Qingli Luo, Hongying Zhang, Baojun Liu, Fei Xu, Qi Pang, Yingchao Liu, Jingcheng Dong
Pro-inflammatory cytokines play a crucial role in the etiology of atopic dermatitis. We demonstrated that Herba Epimedii has anti-inflammatory potential in an atopic dermatitis mouse model; however, limited research has been conducted on the anti-inflammatory effects and mechanism of icariin, the major active ingredient in Herba Epimedii, in human keratinocytes. In this study, we evaluated the anti-inflammatory potential and mechanisms of icariin in the tumor necrosis factor-α (TNF-α)/interferon-γ (IFN-γ)-induced inflammatory response in human keratinocytes (HaCaT cells) by observing these cells in the presence or absence of icariin...
December 2015: International Immunopharmacology
Dongdong Zhang, Zhe Wang, Chenxia Sheng, Weijun Peng, Shan Hui, Wei Gong, Shuai Chen
Icariin is a prenylated flavonol glycoside derived from the Chinese herb Epimedium sagittatum that exerts a variety of pharmacological activities and shows promise in the treatment and prevention of Alzheimer's disease. In this study, we investigated the neuroprotective effects of icariin against amyloid beta protein fragment 25-35 (Aβ 25-35) induced neurotoxicity in cultured rat pheochromocytoma PC12 cells and explored potential underlying mechanisms. Our results showed that icariin dose-dependently increased cell viability and decreased Aβ 25-35-induced apoptosis, as assessed by MTT assay and Annexin V/propidium iodide staining, respectively...
2015: Evidence-based Complementary and Alternative Medicine: ECAM
Chunrong Jia, Hongxiao Liu, Min Li, Zhikui Wu, Xinghua Feng
The aim of this study is to explore the effects of icariin on cytokine induced ankylosing spondylitis fibroblast osteogenesis type expression and its molecular mechanism. The normal fibroblasts were collected as normal control group, and the fibroblasts of hip joint capsule of AS patients were collected, which were respectively added in fetal bovine serum (group AS), fetal bovine serum and cytokines (BMP-2+TGF-beta 1) (group AS), and cell factor solution (icariin group), and observed of the osteogenic expression of fibroblast, to evaluate the impact of Icariin on it...
2014: International Journal of Clinical and Experimental Pathology
Ting Zhang, Zhanwei Zhang, Keli Dong, Guangcheng Li, Hong Zhu
One of our previous studies showed that Yizhijiannao Granule, a compound Chinese medicine, effectively improved the clinical symptoms of Alzheimer's disease. In the present study, we established a model of Alzheimer's disease using beta-amyloid (25-35) in PC12 cells, and treated the cells with Yizhijiannao Granule and its four monomers, i.e., icariin, catechin, Panax notoginseng saponins, and eleutheroside E. Flow cytometry showed that Yizhijiannao Granule-containing serum, icariin, Panax notoginseng saponins, and icariin + Panax notoginseng saponins were protective against beta-amyloid (25-35)-induced injury in PC12 cells...
August 25, 2012: Neural Regeneration Research
Wen-Gui Shi, Xiao-Ni Ma, Yan-Fang Xie, Jian Zhou, Jian Zhou
OBJECTIVE: To investigate the estrogenic activity of icariin and genistein with estrogen-dependent human breast cancer (MCF-7) cells. METHOD: MCF-7 cells were incubated with media containing 5% charcoal dextran-treated FBS in phenol red-free media for 48 h. CCK-8 kit was used to study the impact of defferent concentration of icariin and genistein on MCF-7 proliferation in vitro. Optimal concentration icariin and genistein were added into medium and total RNA was isolated after 12, 24, 36, 48 h...
July 2014: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Li Zeng, Wei Wang, Xiao-Feng Rong, Yu Zhong, Ping Jia, Guo-Qing Zhou, Rong-Heng Li
Cartilage degradation is the most predominant pathological change during osteoarthritis (OA). Furthermore, accumulating evidence suggests that an excess of matrix metalloproteinase-13 (MMP-13) plays a critical role in the breakdown of cartilage. Here, the effects of Icariin on the expression of MMP-13 in IL-1β-induced SW 1353 chondrosarcoma cells were investigated. In addition, the in vivo effects of Icariin on an experimental rat model of OA induced by anterior cruciate ligament transection (ACLT) was examined...
January 2014: International Immunopharmacology
Jian Zhou, Ke-Ming Chen, Bao-Feng Ge, Xiao-Ni Ma, Xiao-Yu Guo, Kui Cheng, Yu-Hai Gao, Li-Juan Yan, Wen-Gui Shi
OBJECTIVE: To compare the effect of icariin and genistein in the osteogenic differentiation of rat bone marrow stromal cells (rBMSC). METHOD: Rat marrow stromal cells were seperated in vitro, and the optimal concentration of genisten and icriin were screened. Genistein and icariin with the concentration of 1 x 10(-5) mol x L(-1) were adopted to intereven rBMSCs cultured in vitro. Alkaline phosphatase (ALP) was determined at 3, 6, 9, 12,15 d after intervention; calcified nodule was detected with alizarin red staining at 12 d; OXS, Runx-2, bone morphogenetic protein (BMP-2) and Collagen-I mRNA expression were observed with Real-time RT-PCR at 12, 24, 48, 72, 96 h...
June 2013: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Xuehui Zhang, Mingming Xu, Lin Song, Yan Wei, Yuanhua Lin, Wentao Liu, Boon C Heng, Hui Peng, Ying Wang, Xuliang Deng
Combinations of calcium phosphate scaffolds and bioactive factors are promising niche-mimetic solutions for repairing large-sized bone defects. However, the importance of compatibility between scaffolds and bioactive factors on their osteogenic outcomes has been largely ignored. This study aimed to investigate the compatibility of calcinated antler cancellous bone (CACB) scaffolds with various bioactive factors including icariin (ICA), velvet antler polypeptides (VAP) or recombinant human bone morphogenetic protein-2 (rhBMP-2) as well as their combinational osteogenic potential in vitro and in vivo...
December 2013: Biomaterials
Duan Xiao-Hong, Xu Chang-Qin, Huang Jian-Hua, Zhou Wen-Jiang, Sun Bing
CONTEXT: Homocysteine-induced endothelial cellular senescence may contribute to some cardiovascular disorders. Icariin (ICA), a flavonoid derived from Epimedium sagittatum Maxim. (Berberidaceae), has been reported to increase production of nitric oxide (NO) and reduce reactive oxygen species (ROS) levels in human umbilical vein endothelial cells (HUVECs). OBJECTIVE: To observe the effects of ICA on homocysteine-induced senescence and the underlying mechanisms in HUVECs...
April 2013: Pharmaceutical Biology
Ye Zhang, Qiang-Song Wang, Yuan-Lu Cui, Fan-Cui Meng, Ke-Ming Lin
Icariin is a bioactive herbal ingredient isolated from Herba epimedii, which has been widely used for the treatment of osteoporosis and male sexual dysfunction in traditional Chinese medicine. The major objective of this work is to investigate the different enhancing effects of β-cyclodextrin (β-CD) and hydroxypropyl-β-cyclodextrin (HP-β-CD) on the intestinal absorption of icariin, and to identify the molecular mechanisms of this action. Host-guest-type interactions of icariin with cyclodextrins nanocavities were unambiguously demonstrated by the phase-solubility diagram, ultraviolet spectroscopy, Fourier transform infrared spectroscopy, differential scanning calorimetry, X-ray powder diffractometry, and two dimensional proton nuclear magnetic resonance rotating-frame Overhauser effect spectroscopy...
2012: International Journal of Nanomedicine
Takuya Urano, Chihiro Tohda
Essential therapeutic drugs for Alzheimer's disease (AD) have not been developed. Since the neuritic atrophy leading to synaptic losses is one of the critical causes of memory impairment in AD, the effects of several constituents in tonic herbal medicines on neuritic atrophy and memory deficits have been studied. The present study investigated the effects of icariin, a main constituent in Epimedii Herba, a well known tonic crude drug, in an in vitro AD model and transgenic mouse AD model (5xFAD). Amyloid β(1-42)-induced atrophies of axons and dendrites were restored by post-treatment with icariin in rat cortical neurons...
November 2010: Phytotherapy Research: PTR
Ke-Wu Zeng, Hyeonseok Ko, Hyun Ok Yang, Xue-Mei Wang
Alzheimer's disease (AD) is a neurodegenerative disease characterized by the progressive loss of neurons and production of β-amyloid proteins (Aβ). Hyperphosphorylation of tau protein is proposed to be an early event for the evolution of AD, and may play an important role in Aβ-induced neurodegeneration. Icariin, a flavonoid compound from the herb Epimedium brevicornum Maxim, exerts a protective effect on learning and memory abilities in Aβ(25-35)-induced AD rats. However, the molecular mechanism of icariin-induced neuroprotective effect against tau protein hyperphosphorylation, which is one of the most representative hallmarks in AD, is still unknown...
November 2010: Neuropharmacology
Hao Wu, Zhen-gang Zha, Ping Yao
OBJECTIVE: To research the effect of icariin (ICA) on the proliferation and differentiation of bone mesenchymal stem cells (BMSCs) and to study its influence on the expressions of transforming growth factor-beta1 (TGF-beta1) and bone morphogenetic protein-2 (BMP-2) in the progress of BMSCs differentiating into osteoblast, for providing an experimental evidence to explain the mechanism of ICA, also for exploring the feasibility of establishing a platform upon TGF-beta, and BMP-2 to screen out the medicine in preventing and treating osteoporosis...
April 2010: Chinese Journal of Integrated Traditional and Western Medicine
Sao-Keng Mok, Wen-Fang Chen, Wan-Ping Lai, Ping-Chung Leung, Xin-Luan Wang, Xin-Sheng Yao, Man-Sau Wong
BACKGROUND AND PURPOSE: Icariin may be the active ingredient in Herba Epimedii, a Chinese herb commonly used for treatment of osteoporosis. The present study aims to delineate the mechanism(s) by which icariin prevents bone loss after ovariectomy (OVX) in vivo and stimulates osteoblastic functions in vitro. EXPERIMENTAL APPROACH: Ovariectomized or sham-operated C57BL/6 mice were treated with vehicle, 17beta-oestradiol or icariin for 6 weeks. Total and trabecular bome mineral density (BMD) as well as polar stress-strain index of distal femur were measured by peripheral computed tomography...
February 2010: British Journal of Pharmacology
Li Li, Hao-Jan Tsai, Lin Li, Xue-Mei Wang
Overload of intracellular calcium caused by amyloid-beta peptide has been implicated in the pathogenesis of neuronal damage in Alzheimer's disease. Voltage-gated calcium channels (VGCCs) provide one of the major sources of Ca(2+) entry into cells. Here, we investigated whether icariin had effect on the changes of calcium currents induced by Abeta(25-35) in hippocampal pyramidal neurons. Using whole-cell patch-clamp, we showed that Abeta(25-35) enhanced the inward Ba(2+) and Ca(2+) currents. The currents were partially inhibited by Ni(2+) and completely suppressed by Cd(2+), indicating that Abeta(25-35) disrupts intracellular calcium homeostasis via the modulation of both L- and T-type channels...
2010: American Journal of Chinese Medicine
Wen-jun Wang, Da-jin Li, Jun Li, Wen-jiang Zhou
OBJECTIVE: To observe the effects of serum containing Gengnianchun (GNC) decoction, a compound traditional Chinese herbal medicine, and its monomers (paeoniflorin, berberine, timosaponin A-III and icariin) on neurotoxicity in PC12 cells induced by amyloid beta-protein (Abeta). METHODS: Injury of PC12 cells was induced by incubating with Abeta(25-35) in vitro. Ovariectomized rats were intragastrically administered with GNC decoction twice daily for 5 days and then sera were obtained...
January 2010: Zhong Xi Yi Jie He Xue Bao, Journal of Chinese Integrative Medicine
Dujuan Sha, Lingling Li, Lan Ye, Rong Liu, Yun Xu
beta-Amyloid peptide (Abeta) is one of the main protein components of senile plaques contributing to Alzheimer's disease and it can induce neuronal apoptosis. In this study, it was found that icariin, a flavonoid extracted from Chinese Herba-Epimedii, inhibited Abeta42-induced neurotoxicity in a dose-dependent manner. The peak dose of icariin was 160 microg/ml. In addition, mRNA and protein expressions of cocaine and amphetamine-regulated transcript (CART) were increased in Abeta42-treated neurons in the presence of 80 microg/ml icariin...
November 25, 2009: Neuroreport
Jun-Seong Park, Hye Yoon Park, Ho-Sik Rho, Soomi Ahn, Duck Hee Kim, Ih Seop Chang
Three kinds of prenylated flavonols, icariside I, icariside II, and icaritin, were isolated from an icariin hydrolysate and their effects on melanogenesis evaluated based on mushroom tyrosinase inhibition and quantifying the melanin contents in melanocytes. Although none of the compounds had an effect on tyrosinase activity, icariside II and icaritin both effectively inhibited the melanin contents with an IC50 of 10.53 and 11.13 MM, respectively. Whereas icariside II was obtained from a reaction with beta-glucosidase and cellulase, the icariin was not completely converted into icariside II...
January 2008: Journal of Microbiology and Biotechnology
P Shen, B L Guo, Y Gong, Deborah Y Q Hong, Y Hong, E L Yong
To understand the factors contributing to estrogenic properties of extracts from the genus Epimedium L. (Berberidaceae), we performed taxonomic, genetic and chemical characterization on 37 specimens from 18 species and related these to estrogen receptor (ERalpha and ERbeta) bioactivity, as measured by reporter genes in stable human cells. Boot strap values derived from amplified fragment length polymorphisms indicated that specimens of E. koreanum, E. brevicornum, E. myrianthum, E. leishanense, and E. membranaceum were genetically distinct and this was supported by their very similar ERalpha activities...
May 2007: Phytochemistry
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