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Tumour budding

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https://www.readbyqxmd.com/read/29040332/the-developmental-programme-for-genesis-of-the-entire-kidney-is-recapitulated-in-wilms-tumour
#1
Ryuji Fukuzawa, Matthew R Anaka, Ian M Morison, Anthony E Reeve
Wilms tumour (WT) is an embryonal tumour that recapitulates kidney development. The normal kidney is formed from two distinct embryological origins: the metanephric mesenchyme (MM) and the ureteric bud (UB). It is generally accepted that WT arises from precursor cells in the MM; however whether UB-equivalent structures participate in tumorigenesis is uncertain. To address the question of the involvement of UB, we assessed 55 Wilms tumours for the molecular features of MM and UB using gene expression profiling, immunohistochemsitry and immunofluorescence...
2017: PloS One
https://www.readbyqxmd.com/read/29040282/colorectal-carcinoma-tumour-budding-and-podia-formation-in-the-xenograft-microenvironment
#2
Friedrich Prall, Claudia Maletzki, Maja Hühns, Mathias Krohn, Michael Linnebacher
Tumour budding and podia formation are well-appreciated in surgical pathology as an aggressive invasion phenotype of colorectal carcinoma cells that is attained in the microenvironment of the invasive margin. In this study, we addressed how tumour budding and podia formation feature in xenografts. Primary colorectal carcinomas (N = 44) of various molecular types (sporadic standard type, high-degree microsatellite-unstable, CpG island methylator phenotype) were transplanted subcutaneously into T and B cell-deficient NSG mice, making possible immunohistochemistry with routine surgical pathology antibodies...
2017: PloS One
https://www.readbyqxmd.com/read/28881357/a-novel-inhibitory-anti-invasive-mab-isolated-using-phenotypic-screening-highlights-anxa6-as-a-functionally-relevant-target-protein-in-pancreatic-cancer
#3
Dermot O'Sullivan, Paul Dowling, Helena Joyce, Edel McAuley, Andrew McCann, Michael Henry, Brianan McGovern, Paul Barham, Fergal C Kelleher, Jean Murphy, Susan Kennedy, Niall Swan, Michael Moriarty, Martin Clynes, Annemarie Larkin
BACKGROUND: Discovery and validation of new antibody tractable targets is critical for the development of new antibody therapeutics to address unmet needs in oncology. METHODS: A highly invasive clonal variant of the MDA-MB-435S cell line was used to generate monoclonal antibodies (MAbs), which were screened for anti-invasive activity against aggressive cancer cells in vitro. The molecular target of selected inhibitory MAb 9E1 was identified using immunoprecipitation/liquid chromatography-tandem mass spectrometry...
October 24, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28770101/cytokeratin-based-assessment-of-tumour-budding-in-colorectal-cancer-analysis-in-stage-ii-patients-and-prospective-diagnostic-experience
#4
Viktor H Koelzer, Naziheh Assarzadegan, Heather Dawson, Bojana Mitrovic, Andrea Grin, David E Messenger, Richard Kirsch, Robert H Riddell, Alessandro Lugli, Inti Zlobec
Tumour budding in colorectal cancer is an important prognostic factor. A recent consensus conference elaborated recommendations and key issues for future studies, among those the use of pan-cytokeratin stains, especially in stage II patients. We report the first prospective diagnostic experience using pan-cytokeratin for tumour budding assessment. Moreover, we evaluate tumour budding using pan-cytokeratin stains and disease-free survival (DFS) in stage II patients. To this end, tumour budding on pan-cytokeratin-stained sections was evaluated by counting the number of tumour buds in 10 high-power fields (0...
July 2017: Journal of Pathology. Clinical Research
https://www.readbyqxmd.com/read/28763204/effect-of-kumatakenin-isolated-from-cloves-on-the-apoptosis-of-cancer-cells-and-the-alternative-activation-of-tumor-associated-macrophages
#5
Jeong-Hwa Woo, Ji-Hye Ahn, Dae Sik Jang, Kyung-Tae Lee, Jung-Hye Choi
The flower bud of Syzygium aromaticum (clove) has been used for centuries as a spice and herbal medicine. The biological activities of kumatakenin, a flavonoid that has recently been isolated from cloves, are poorly characterized. In the present study, the anticancer effects of kumatakenin in human ovarian cancer cells and tumor-associated macrophages (TAMs) were investigated. We found that kumatakenin exhibited significant cytotoxic activity in human ovarian cancer cells, SKOV3 and A2780. A propidium iodide and Annexin V-FITC staining assay showed that kumatakenin induces apoptosis in ovarian cancer cells...
September 13, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28524540/antineoplastic-effects-of-clove-buds-syzygium-aromaticum-l-in-the-model-of-breast-carcinoma
#6
Peter Kubatka, Sona Uramova, Martin Kello, Karol Kajo, Peter Kruzliak, Jan Mojzis, Desanka Vybohova, Marian Adamkov, Karina Jasek, Zora Lasabova, Pavol Zubor, Silvia Fialova, Svetlana Dokupilova, Peter Solar, Martin Pec, Katarina Adamicova, Jan Danko, Mariusz Adamek, Dietrich Busselberg
It is supposed that plant functional foods, rich in phytochemicals, may potentially have preventive effects in carcinogenesis. In this study, the anticancer effects of cloves in the in vivo and in vitro mammary carcinoma model were assessed. Dried flower buds of cloves (CLOs) were used at two concentrations of 0.1% and 1% through diet during 13 weeks after the application of chemocarcinogen. After autopsy, histopathological and immunohistochemical analyses of rat mammary carcinomas were performed. Moreover, in vitro evaluation using MCF-7 cells was carried out...
November 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28427085/tumour-invasiveness-the-local-and-systemic-environment-and-the-basis-of-staging-systems-in-colorectal-cancer
#7
J H Park, H van Wyk, C S D Roxburgh, P G Horgan, J Edwards, D C McMillan
BACKGROUND: The present study aimed to examine the relationship between tumour invasiveness (T stage), the local and systemic environment and cancer-specific survival (CSS) of patients with primary operable colorectal cancer. METHODS: The tumour microenvironment was examined using measures of the inflammatory infiltrate (Klintrup-Makinen (KM) grade and Immunoscore), tumour stroma percentage (TSP) and tumour budding. The systemic inflammatory environment was examined using modified Glasgow Prognostic Score (mGPS) and neutrophil:lymphocyte ratio (NLR)...
May 23, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28415597/phosphorylation-of-androgen-receptors-at-serine-515-is-a-potential-prognostic-marker-for-triple-negative-breast-cancer
#8
Antonia K Roseweir, Pamela McCall, Alison Scott, Benjamin Liew, Zhi Lim, Elizabeth A Mallon, Joanne Edwards
1.7 million cases of breast cancer are diagnosed every year with 522,000 deaths. Molecular classifications of breast cancer have resulted in improved treatments. However, treatments for triple negative breast cancer (TNBC) are lacking. Analysis of molecular targets for TNBC is a priority. One potential candidate is androgen receptor (AR) phosphorylation. This study assessed the role of AR phosphorylation at ser81/ser515 and their two upstream effectors, cyclin-dependent kinase 1 (pCDK1) and extracellular-regulated kinase 1/2 (pERK1/2) in 332 ductal breast cancer patients by immunohistochemistry...
June 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/28276469/engineering-a-vascularised-3d-in-vitro-model-of-cancer-progression
#9
Tarig Magdeldin, Víctor López-Dávila, Judith Pape, Grant W W Cameron, Mark Emberton, Marilena Loizidou, Umber Cheema
The hallmark of tumours is the ability of cancerous cells to promote vascular growth, to disseminate and invade to distant organs. The metastatic process is heavily influenced by the extracellular matrix (ECM) density and composition of the surrounding tumour microenvironment. These microenvironmental cues, which include hypoxia, also regulate the angiogenic processes within a tumour, facilitating the spread of cancer cells. We engineered compartmentalized biomimetic colorectal tumouroids with stromal surrounds that comprised a range of ECM densities, composition and stromal cell populations...
March 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28061021/comprehensive-assessment-of-tumour-budding-by-cytokeratin-staining-in-colorectal-cancer
#10
Gregor Rieger, Viktor H Koelzer, Heather E Dawson, Martin D Berger, Marion Hädrich, Daniel Inderbitzin, Alessandro Lugli, Inti Zlobec
AIMS: Tumour budding in colorectal cancer (CRC) is a recognized prognostic parameter. The aim of this study was to address the use of cytokeratin immunostaining for the visualization and scoring of tumour buds. METHODS AND RESULTS: Ten hotspots (0.238 mm(2) ) of peritumoural budding (PTB) and intratumoural budding (ITB) were evaluated in surgical resections from 215 patients. The budding counts in the 10 densest regions anywhere in the tumour were combined into an overall tumour budding (OTB) score...
January 6, 2017: Histopathology
https://www.readbyqxmd.com/read/27999198/tumour-derived-exosomes-as-a-signature-of-pancreatic-cancer-liquid-biopsies-as-indicators-of-tumour-progression
#11
REVIEW
Zarin Nuzhat, Vyjayanthi Kinhal, Shayna Sharma, Gregory E Rice, Virendra Joshi, Carlos Salomon
Pancreatic cancer is the fourth most common cause of death due to cancer in the world. It is known to have a poor prognosis, mostly because early stages of the disease are generally asymptomatic. Progress in pancreatic cancer research has been slow, leaving several fundamental questions pertaining to diagnosis and treatment unanswered. Recent studies highlight the putative utility of tissue-specific vesicles (i.e. extracellular vesicles) in the diagnosis of disease onset and treatment monitoring in pancreatic cancer...
March 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/27926795/tumour-budding-evaluated-in-biopsy-specimens-is-a-useful-predictor-of-prognosis-in-patients-with-cn0-early-stage-oral-squamous-cell-carcinoma
#12
Mai Seki, Takaaki Sano, Satoshi Yokoo, Tetsunari Oyama
AIMS: Oral squamous cell carcinoma (OSCC) prognosis depends upon lymph node metastasis (LNM). We have reported recently that tumour budding is a good predictive factor for LNM in squamous cell carcinoma (SCC) of the tongue and floor of the mouth (FOM). Our aim was to evaluate whether tumour budding is a good prognostic factor in OSCC. METHODS AND RESULTS: We examined conventional histopathological assessment and a new factor, tumour budding, in 209 cases of OSCC in incisional biopsy specimens...
May 2017: Histopathology
https://www.readbyqxmd.com/read/27887640/functional-and-regulatory-profiling-of-energy-metabolism-in-fission-yeast
#13
Michal Malecki, Danny A Bitton, Maria Rodríguez-López, Charalampos Rallis, Noelia Garcia Calavia, Graeme C Smith, Jürg Bähler
BACKGROUND: The control of energy metabolism is fundamental for cell growth and function and anomalies in it are implicated in complex diseases and ageing. Metabolism in yeast cells can be manipulated by supplying different carbon sources: yeast grown on glucose rapidly proliferates by fermentation, analogous to tumour cells growing by aerobic glycolysis, whereas on non-fermentable carbon sources metabolism shifts towards respiration. RESULTS: We screened deletion libraries of fission yeast to identify over 200 genes required for respiratory growth...
November 25, 2016: Genome Biology
https://www.readbyqxmd.com/read/27884016/expression-profiling-of-budding-cells-in-colorectal-cancer-reveals-an-emt-like-phenotype-and-molecular-subtype-switching
#14
Linde De Smedt, Sofie Palmans, Daan Andel, Olivier Govaere, Bram Boeckx, Dominiek Smeets, Eva Galle, Jasper Wouters, David Barras, Madeleine Suffiotti, Jeroen Dekervel, Thomas Tousseyn, Gert De Hertogh, Hans Prenen, Sabine Tejpar, Diether Lambrechts, Xavier Sagaert
BACKGROUND: Tumour budding, described as the presence of single cells or small clusters of up to five tumour cells at the invasive margin, is established as a prognostic marker in colorectal carcinoma. In the present study, we aimed to investigate the molecular signature of tumour budding cells and the corresponding tumour bulk. METHODS: Tumour bulk and budding areas were microdissected and processed for RNA-sequencing. As little RNA was obtained from budding cells, a special low-input mRNA library preparation protocol was used...
January 3, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/27815460/bubr1-insufficiency-in-mice-increases-their-sensitivity-to-oxidative-stress
#15
Daisuke Matsuda, Takuya Matsumoto, Kenichi Honma, Ayae Ikawa-Yoshida, Mitsuho Onimaru, Tadashi Furuyama, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Yoshihiko Maehara
BACKGROUND/AIM: Budding uninhibited by benzimidazole-related 1 (BUBR1) plays an important role in the spindle assembly checkpoint to prevent chromosome missegregation and aneuploidy during mitosis. We previously generated mutant mice that express BUBR1 at only 20% of the normal level (BubR1(L/L) mice). Here, we examined the effect of low BUBR1 expression on oxidative stress-induced carcinogenesis in mice. MATERIALS AND METHODS: We orally administered either a potassium bromate (KBrO3) solution (2 g/l) or tap water to BubR1(L/L) and wild-type (BubR1(+/+))mice for 16 weeks and examined the subsequent incidence of tumours...
November 2016: In Vivo
https://www.readbyqxmd.com/read/27678454/signal-transduction-and-activator-of-transcription-3-stat3-in-patients-with-colorectal-cancer-associations-with-the-phenotypic-features-of-the-tumor-and-host
#16
James H Park, Hester van Wyk, Donald C McMillan, Jean Quinn, Jennifer Clark, Campbell S D Roxburgh, Paul G Horgan, Joanne Edwards
PURPOSE: In patients with colorectal cancer, a high-density local inflammatory infiltrate response is associated with improved survival, whereas elevated systemic inflammatory responses are associated with poor survival. One potential unifying mechanism is the IL6/JAK/STAT3 pathway. The present study examines the relationship between tumor total STAT3 and phosphorylated STAT3Tyr705 (pSTAT3) expression, host inflammatory responses, and survival in patients undergoing resection of stage I-III colorectal cancer...
September 27, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/27630328/tumour-budding-correlates-with-aggressiveness-of-cutaneous-squamous-cell-carcinoma
#17
Georgia Karayannopoulou, Sylvie Euvrard, Jean Kanitakis
BACKGROUND/AIM: Tumour budding (TB) is a specific pathological feature that has been found to be associated with an aggressive outcome in several cancer types; however, to our knowledge, TB has not yet been assessed in squamous-cell carcinomas of the skin (SCC). The aim of the study was to study whether TB correlates with aggressiveness in cutaneous SCC. MATERIALS AND METHODS: We examined 31 aggressive SCC (that later developed local recurrences or metastases) in comparison with 21 non-aggressive SCC (not complicated by recurrence or metastasis)...
2016: Anticancer Research
https://www.readbyqxmd.com/read/27599041/systematic-review-and-meta-analysis-of-the-impact-of-tumour-budding-in-colorectal-cancer
#18
REVIEW
A C Rogers, D C Winter, A Heeney, D Gibbons, A Lugli, G Puppa, K Sheahan
BACKGROUND: Tumour budding is a histological finding in epithelial cancers indicating an unfavourable phenotype. Previous studies have demonstrated that it is a negative prognostic indicator in colorectal cancer (CRC), and has been proposed as an additional factor to incorporate into staging protocols. METHODS: A systematic review of papers until March 2016 published on Embase, Medline, PubMed, PubMed Central and Cochrane databases pertaining to tumour budding in CRC was performed...
September 27, 2016: British Journal of Cancer
https://www.readbyqxmd.com/read/27404452/characterisation-of-pd-l1-positive-subsets-of-microsatellite-unstable-colorectal-cancers
#19
Jung Ho Kim, Hye Eun Park, Nam-Yun Cho, Hye Seung Lee, Gyeong Hoon Kang
BACKGROUND: The aim of this study was to reveal the clinicopathological and molecular characteristics of microsatellite instability-high (MSI-H) colorectal cancers (CRCs) showing programmed death ligand-1 (PD-L1) positivity, which are good candidates for anti-PD-1/PD-L1 immunotherapy. METHODS: The PD-L1 expression status of 208 MSI-H CRCs was retrospectively analysed using immunohistochemistry. PD-L1 positivity in tumour cells (PD-L1+(T)) and PD-L1 positivity in immune cells (PD-L1+(I)) were separately evaluated...
August 9, 2016: British Journal of Cancer
https://www.readbyqxmd.com/read/27394431/prognostic-relevance-of-histopathological-features-in-signet-ring-cell-carcinoma-of-the-colorectum
#20
Valeria Barresi, Luca Reggiani Bonetti, Federica Domati, Luigi Baron
Signet ring cell carcinoma (SRCC) is a rare variant of colorectal cancer (CRC), by definition composed of at least 50 % of neoplastic cells showing signet ring cell morphology. Colorectal SRCC is mainly characterized by aggressive clinical behavior, high pTNM stage and microsatellite instability (MSI). We assessed the prognostic value of several histopathological parameters (histological grade, venous invasion, lymphovascular invasion, MSI, mucin content, tumour budding, pTNM stage) in terms of disease free survival (DFS) and cancer specific survival (CSS) in a series of 32 SRCCs...
September 2016: Virchows Archiv: An International Journal of Pathology
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