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https://www.readbyqxmd.com/read/28209712/mycobacterium-tuberculosis-esxl-inhibit-mhc-ii-expression-by-promoting-hypermethylation-in-class-ii-transactivator-loci-in-macrophages
#1
Srabasti Sengupta, Saba Naz, Ishani Das, Abdul Ahad, Avinash Padhi, Sumanta Naik, Geetanjali Ganguli, Kaliprasad Patnaik, Sunil Kumar Raghav, Vinay Nandicoori, Avinash Sonawane
Mycobacterium tuberculosis (Mtb) is known to modulate the host immune responses to facilitate its persistence inside the host cells. One of the key mechanisms includes repression of class-II transactivator (CIITA) and MHC-II expression in infected macrophages. However, the precise mechanism of CIITA and MHC-II down-regulation is not well studied. Mtb 6-kDa early secretory antigenic target (ESAT-6) is a known potent virulence and antigenic determinant. Mtb genome encodes 23 such ESAT-6 family proteins. We herein report that Mtb and M...
February 16, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28205272/homometallic-dyiii-complexes-of-varying-nuclearity-from-2-to-21-synthesis-structure-and-magnetism
#2
Vadapalli Chandrasekhar, Sourav Biswas, Sourav Das, Joydev Acharya, Vierandra Kumar, Jan Leusen, Juan Manuel Herrera, Paul Koegerler, Enrique Colacio
The synthesis, structure and magnetic properties of the four Dy(III) coordination compounds isolated as [Dy2(LH2)2(μ2-η1:η1-Piv)]Cl·2MeOH·H2O (1), [Dy4(LH)2(μ3-OH)2(Piv)4(MeOH)2]·4MeOH·2H2O(2), [Dy6(LH2)3(tfa)3(O3PtBu)(Cl)3]Cl4·15.5H2O·4MeOH· 5CHCl3 (3) and [Dy21(L)7(LH)7(tfa)7]Cl7·15H2O·7MeOH·12CHCl3 (4) are reported (Piv = pivalate, tfa = 1,1,1-trifluoro-acetylacetone, O3PtBu = tert-butylphosphonate). Some of these polynuclear coordination complexes exhibit aesthetic and unprecedented structural features...
February 16, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28196656/a-cdna-clone-launched-platform-for-high-yield-production-of-inactivated-zika-vaccine
#3
Yujiao Yang, Chao Shan, Jing Zou, Antonio E Muruato, Diniz Nunes Bruno, Barbosa de Almeida Medeiros Daniele, Pedro F C Vasconcelos, Shannan L Rossi, Scott C Weaver, Xuping Xie, Pei-Yong Shi
A purified inactivated vaccine (PIV) using the Zika virus (ZIKV) Puerto Rico strain PRVABC59 showed efficacy in monkeys, and is currently in a phase I clinical trial. High-yield manufacture of this PIV is essential for its development and vaccine access. Here we report an infectious cDNA clone-launched platform to maximize its yield. A single NS1 protein substitution (K265E) was identified to increase ZIKV replication on Vero cells (a cell line approved for vaccine production) for both Cambodian FSS13025 and Puerto Rico PRVABC59 strains...
February 6, 2017: EBioMedicine
https://www.readbyqxmd.com/read/28196148/from-the-track-to-the-ocean-using-flow-control-to-improve-marine-bio-logging-tags-for-cetaceans
#4
Giovani Fiore, Erik Anderson, C Spencer Garborg, Mark Murray, Mark Johnson, Michael J Moore, Laurens Howle, K Alex Shorter
Bio-logging tags are an important tool for the study of cetaceans, but superficial tags inevitably increase hydrodynamic loading. Substantial forces can be generated by tags on fast-swimming animals, potentially affecting behavior and energetics or promoting early tag removal. Streamlined forms have been used to reduce loading, but these designs can accelerate flow over the top of the tag. This non-axisymmetric flow results in large lift forces (normal to the animal) that become the dominant force component at high speeds...
2017: PloS One
https://www.readbyqxmd.com/read/28186414/a-three-pronged-attack-to-investigate-the-electronic-structure-of-a-family-of-ferromagnetic-fe4ln2-cyclic-coordination-clusters-a-combined-magnetic-susceptibility-high-field-high-frequency-electron-paramagnetic-resonance-and-57-fe-m%C3%A3-ssbauer-study
#5
Sebastian F M Schmidt, Changhyun Koo, Valeriu Mereacre, Jaena Park, Dieter W Heermann, Vladislav Kataev, Christopher E Anson, Denis Prodius, Ghenadie Novitchi, Rüdiger Klingeler, Annie K Powell
We present the synthesis, structure, magnetic properties, as well as the Mössbauer and electron paramagnetic resonance studies of a ring-shaped [Fe(III)4Ln(III)2(Htea)4(μ-N3)4(N3)3(piv)3] (Ln = Y 1, Gd 2, Tb 3, Dy 4, Ho 5, Er, 6) coordination cluster. The Dy, Tb, and Ho analogues show blocking of the magnetization at low temperatures without applied fields. The anisotropy of the 3d ion and the exchange interaction between 3d and 4f ions in Fe4Ln2 complexes are unambiguously determined by high-field/high-frequency electron paramagnetic resonance measurements at low temperature...
February 10, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28169811/am%C3%A3-lioration-de-la-mesure-d%C3%A2-extravasation-des-cath%C3%A3-ters-veineux-p%C3%A3-riph%C3%A3-riques-par-les-infirmi%C3%A3-res-en-p%C3%A3-diatrie-gr%C3%A3-ce-%C3%A3-l%C3%A2-utilisation-d%C3%A2-un-instrument-d%C3%A2-%C3%A3-valuation-sp%C3%A3-cifique
#6
Nathalie Bochaton, Anne Sylvie Ramelet, Peter Rimensberger, Jean-Marie Januel
<ce:para>In pediatric and neonatal intensive care units, severity assessment of extravasation is difficult, considering the specificity of this population. The purpose of this study was to demonstrate the ability to improve the measurement of extravasation by nurses with the establishment of a standardized instrument and suitable for children. 66 nurses, randomly assigned to two groups, assessed the severity of extravasations using 15 clinical vignettes.The intervention group with the Pediatric Peripheral Intravenous Infiltration Scale (PIV Scale) (n=33) and the control group based on clinical judgment only (n=33)...
October 27, 2016: Recherche en Soins Infirmiers
https://www.readbyqxmd.com/read/28159006/the-potential-influence-of-human-parainfluenza-viruses-detected-during-hospitalization-among-critically-ill-patients-in-kuwait-2013-2015
#7
Sahar Essa, Haya Al-Tawalah, Sarah AlShamali, Widad Al-Nakib
BACKGROUND: The four types of human parainfluenza viruses (PIV) are important causes of community-acquired pneumonia, particularly in children; however, limited information exists about the incidence of PIV in critically ill patients. The aim of this study is to describe the spectrum, incidence and clinical features of PIV-associated infections diagnosed during the hospital stay of patients admitted to pediatric intensive care unit (PICU) and intensive care unit (ICU) of 5 medical centers across Kuwait...
February 3, 2017: Virology Journal
https://www.readbyqxmd.com/read/28144057/respiratory-viruses-in-acute-exacerbations-of-chronic-obstructive-pulmonary-disease
#8
Parvaiz A Koul, Hyder Mir, Shabir Akram, Varsha Potdar, Mandeep S Chadha
OBJECTIVE: Acute exacerbations of chronic obstructive pulmonary disease (AECOPD) cause significant morbidity, mortality, and an inexorable decline of lung function. Data from developed countries have shown viruses to be important causes of AECOPD, but data from developing countries like India are scant. We set out to determine the contribution of viruses in the causation of hospitalized patients with AECOPD. METHODS: Twin nasopharyngeal/oropharyngeal swabs collected from 233 patients admitted with an acute AECOPD and tested for respiratory viruses including respiratory syncytial virus A and B, parainfluenza were (PIV) 1, 2, 3, and 4, human metapneumovirus (hMPV) A and B, influenza A and B, enterovirus, corona NL65, OC43, and 229E viruses, adenovirus 2 and 4, rhinovirus, and bocavirus, by duplex real time reverse-transcription polymerase chain reaction (qRT-PCR) using CDC approved primers and probes...
January 2017: Lung India: Official Organ of Indian Chest Society
https://www.readbyqxmd.com/read/28140436/en-route-intraosseous-access-performed-in-the-combat-setting
#9
Shelia Savell, Alejandra G Mora, Crystal A Perez, Vikhyat S Bebarta, Maj Joseph K Maddry
OBJECTIVE: To describe and compare vascular access practices used by en route care providers during medical evacuation (MEDEVAC). DESIGN: This was a retrospective cohort study. Medical records of US military personnel injured in combat and transported by MEDEVAC teams were queried. PATIENTS: The subjects were transported by military en route care providers, in the combat theater during Operation Enduring Freedom (OEF) between January 2011 and March 2014...
October 2016: American Journal of Disaster Medicine
https://www.readbyqxmd.com/read/28135577/piv-for-the-characterization-of-focused-field-induced-acoustic-streaming-seeding-particle-choice-evaluation
#10
Rafika Ben Haj Slama, Bruno Gilles, Maher Ben Chiekh, Jean-Christophe Béra
This research evaluates the use of Particle Image Velocimetry (PIV) technique for characterizing acoustic streaming flow generated by High Intensity Focused Ultrasound (HIFU). PIV qualification tests, focusing on the seeding particle size (diameter of 5, 20 and 50μm) were carried out in degassed water subjected to a focused field of 550kHz-frequency with an acoustic pressure amplitude of 5.2, 10.5 and 15.7bar at the focus. This study shows that the ultrasonic field, especially the radiation force, can strongly affect seeding particle behavior...
January 14, 2017: Ultrasonics
https://www.readbyqxmd.com/read/28117807/a-microfluidic-system-with-surface-patterning-for-investigating-cavitation-bubble-s-cell-interaction-and-the-resultant-bioeffects-at-the-single-cell-level
#11
Fenfang Li, Fang Yuan, Georgy Sankin, Chen Yang, Pei Zhong
In this manuscript, we first describe the fabrication protocol of a microfluidic chip, with gold dots and fibronectin-coated regions on the same glass substrate, that precisely controls the generation of tandem bubbles and individual cells patterned nearby with well-defined locations and shapes. We then demonstrate the generation of tandem bubbles by using two pulsed lasers illuminating a pair of gold dots with a few-microsecond time delay. We visualize the bubble-bubble interaction and jet formation by high-speed imaging and characterize the resultant flow field using particle image velocimetry (PIV)...
January 10, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28112331/-detection-of-parainfluenza-virus-in-immunocompromised-and-immunocompetent-patients-in-a-tertiary-hospital-in-sao-paulo-brazil
#12
Sheila N Parmezan, Clarice Camargo, Aripuanã Watanabe, Patricio Godoy-Martínez, Celso Granato, Nancy Bellei
Parainfluenza virus infections (PIV) were evaluated in patients with mild and severe infections through real time PCR. One thousand and sixty-seven samples were collected from subjects as follows: 233 adult renal transplanted outpatients, 129 children with congenital heart disease, 381 with adult hematopoietic stem cell patients and 324 hospitalized patients suspected of influenza A (H1N1) pdm09 infection. PIV was detected in 74 (6.9%) samples. VPI-3 was the most frequent (60.8%) and a higher risk was observed for older adults (p = 0...
October 2016: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
https://www.readbyqxmd.com/read/28102532/time-to-epinephrine-in-out-of-hospital-cardiac-arrest-a-retrospective-analysis-of-intraosseous-versus-intravenous-access
#13
Elliot M Ross, Julian Mapp, Chetan Kharod, David A Wampler, Christopher Velasquez, David A Miramontes
INTRODUCTION: The 2015 advanced cardiac life support update continues to advocate administering epinephrine during cardiac arrest. The goal of our study is to determine if prehospital intraosseous (IO) access results in shorter time to epinephrine than prehospital peripheral intravenous (PIV) access. METHODS: The out-of-hospital cardiac arrest (OHCA) database of a large, urban, fire-based emergency medical services system was searched for consecutive cases of OHCA between January 2013 and December 2015...
April 2016: American Journal of Disaster Medicine
https://www.readbyqxmd.com/read/28074925/atomization-of-high-viscosity-fluids-for-aromatherapy-using-micro-heaters-for-heterogeneous-bubble-nucleation
#14
Junhui Law, Ka Wai Kong, Ho-Yin Chan, Winston Sun, Wen Jung Li, Eric Boa Fung Chau, George Kak Man Chan
The development of a novel lead-free microelectromechanical-system (MEMS)-based atomizer using the principle of thermal bubble actuation is presented. It is a low-cost, lead-free design that is environmentally friendly and harmless to humans. It has been tested to be applicable over a wide range of fluid viscosities, ranging from 1 cP (e.g., water) to 200 cP (e.g., oil-like fluid) at room temperature, a range that is difficult to achieve using ordinary atomizers. The results demonstrate that the average power consumption of the atomizer is approximately 1 W with an atomization rate of 0...
January 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28072527/rational-synthesis-and-investigation-of-porous-metal-organic-framework-materials-from-a-preorganized-heterometallic-carboxylate-building-block
#15
Aleksandr A Sapianik, Ekaterina N Zorina-Tikhonova, Mikhail A Kiskin, Denis G Samsonenko, Konstantin A Kovalenko, Alexey A Sidorov, Igor L Eremenko, Danil N Dybtsev, Alexander J Blake, Stephen P Argent, Martin Schröder, Vladimir P Fedin
The tetranuclear heterometallic complex [Li2Zn2(piv)6(py)2] (1, where piv(-) = pivalate and py = pyridine) has been successfully employed as a presynthesized node for the construction of four porous metal-organic frameworks (MOFs) [Li2Zn2(R-bdc)3(bpy)]·solv (2-R, R-bdc(2-); R = H, Br, NH2, NO2) by reaction with 4,4'-bipyridine (bpy) and terephthalate anionic linkers. The [Li2Zn2] node is retained in the products, representing a rare example of the rational step-by-step design of isoreticular MOFs based on complex heterometallic building units...
January 10, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28061942/a-study-on-hydrodynamic-behavior-of-fine-sediment-in-retention-structure-using-particle-image-velocimetry
#16
Masoumeh MoayeriKashani, Lai Sai Hin, Shaliza Ibrahim, Nik Meriam Nik Sulaiman
  Detention ponds are one type of stormwater best management practices (BMPs) that can be used for the quantity control of stormwater. The pond works by reducing peak discharge downstream through temporary storage and gradual release. In this paper, a new experimental procedure using particle image velocimetry (PIV) is introduced for a two-dimensional study of the hydrodynamic behavior of fine particles in a specially designed sediment basin. The hydrodynamic behavior of fine particles under different hydraulics parameters was achieved by shifting outlet placement, using different particle-size diameters, varying the distance of the inlet from the water surface, and varying the flow rate...
December 1, 2016: Water Environment Research: a Research Publication of the Water Environment Federation
https://www.readbyqxmd.com/read/28060016/excess-respiratory-viral-infections-and-low-antibody-responses-among-hiv-exposed-uninfected-infants
#17
Adriana Weinberg, Marisa M Mussi-Pinhata, Qilu Yu, Rachel A Cohen, Volia C Almeida, Fabiana Amaral, Jorge Pinto, Maria L B Teixeira, Regina C M Succi, Laura Freimanis, Jennifer S Read, George Siberry
OBJECTIVE: HIV-exposed uninfected infants (HEUs) have frequent severe infection, hospitalization and death. We performed a serologic investigation to determine the role of common childhood respiratory pathogens in the excess incidence of infections in HEUs. DESIGN: Prospective cohort study of mother-infant pairs. METHODS: Among 247 HEUs and 88 HIV-unexposed uninfected (HUU) infant-mother pairs, we measured maternal antibodies to respiratory syncytial virus (RSV) and pneumococcus (PNC 1, 5, 6B, 14); infant antibodies to RSV, influenza A (Flu), parainfluenza viruses (PIV 1, 2, 3), and PNC 1, 5, 6B and 14 were measured at 0 and 6 months, and anti-tetanus antibodies at 6 months...
January 4, 2017: AIDS
https://www.readbyqxmd.com/read/28050702/selective-induction-of-il-1%C3%AE-after-a-brief-isoflurane-anesthetic-in-children-undergoing-mri-examination
#18
Emmett E Whitaker, Fievos L Christofi, Kristen M Quinn, Brianne Z Wiemann, Jason C Xia, Joseph D Tobias, Bruno Bissonnette
PURPOSE: To determine if isoflurane anesthesia without surgery causes systemic inflammation in children. Inflammation is targeted as responsible for the development of many neurologic pathologies. The effect will be evaluated by measuring serum cytokine levels before and after isoflurane anesthesia. The possible neurotoxic effect of anesthetic agents is a concern in pediatric anesthesia. Questions remain as to the true effects of anesthesia alone on systemic inflammation. The current study assesses systemic inflammatory response to general anesthesia in children not exposed to surgical stress...
January 3, 2017: Journal of Anesthesia
https://www.readbyqxmd.com/read/28050372/blood-contamination-of-the-small-bore-peripheral-intravenous-catheter-in-neonates
#19
Vani Krishnamurthy, Srinivasa Murthy Doreswamy, Sushma Krishnagowda
INTRODUCTION: Peripheral Intravenous Catheters (PIV) are extensively used in sick neonates for administration of medicines and nutrition. When these PIVs are used on intermittent basis, they are flushed with saline in order to keep the hub of the catheter free from blood. Presence of blood in the hub of the catheter can be potentially dangerous as it could facilitate infection. AIM: The aim of this study was to find the magnitude of blood contamination of PIV catheter hub after routine flushing...
November 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28048396/su-f-t-455-is-contouring-the-whole-breast-necessary-for-two-field-3d-breast-planning
#20
A Desai, Eric Ku, D Fang, C Lawrence, C Iannuzzi, C Shi
PURPOSE: To investigate the effect of contouring the whole breast on reducing the radiation dose to the heart and affected lung in tangential field-in-field 3D breast planning. We hypothesize that contouring the whole breast will simplify the plan normalization process, reduce dose to critical structures, while maintaining treatment plan quality and consistency. METHODS: Twenty previously treated breast cancer patients using tangential field-in-field 3D planning technique were randomly selected...
June 2016: Medical Physics
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