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https://www.readbyqxmd.com/read/28329354/clinical-significance-of-human-coronavirus-in-bronchoalveolar-lavage-samples-from-hematopoietic-cell-transplantation-recipients-and-patients-with-hematologic-malignancies
#1
Chikara Ogimi, Alpana A Waghmare, Jane M Kuypers, Hu Xie, Cecilia C Yeung, Wendy M Leisenring, Sachiko Seo, Su-Mi Choi, Keith R Jerome, Janet A Englund, Michael Boeckh
Background: The possible role of human coronaviruses (HCoV) in lower respiratory tract disease (LRTD) in hematopoietic cell transplant (HCT) recipients and patients with hematologic malignancies (HM) has not been well studied. Methods: We conducted a retrospective review of HCT/HM patients with HCoV detected in bronchoalveolar lavage (BAL). HCoV strains were identified in BAL samples using strain-specific PCR. Mortality rates were compared among HCT recipients with LRTD caused by HCoV, respiratory syncytial virus (RSV), influenza or parainfluenza virus (PIV) by multivariable Cox regression analysis...
February 17, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28325304/improved-resistance-of-chemically-modified-nanocellulose-against-thermally-induced-depolymerization
#2
Melissa B Agustin, Fumiaki Nakatsubo, Hiroyuki Yano
The study demonstrated the improvement in the resistance of nanocellulose against thermally-induced depolymerization by esterification with benzoyl (BNZ) and pivaloyl (PIV). The change in the degree of polymerization (DP) and molecular weight distribution (MWD) after thermal treatment in nitrogen and in air was investigated using viscometry and gel permeation chromatography. BNZ and PIV nanocellulose esters without α-hydrogens gave higher DP and narrower MWD than pure bacterial cellulose; and the acetyl and myristoyl esters, which possess α-hydrogens...
May 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28314051/electroosmotic-flow-measurements-in-a-freely-suspended-liquid-film-experiments-and-numerical-simulations
#3
Abdulkadir Hussein Sheik, H C Hemaka Bandulasena, Victor Starov, Anna Trybala
Fluid flow profiles in free liquid films stabilised by anionic and cationic surfactants under an external electric field were investigated. Depthwise velocity fields were measured at the mid region of the free liquid film by confocal μ-PIV and corresponding numerical simulations were performed using Finite Element Method (FEM) to model the system. Depthwise change in velocity profiles was observed with electroosmotic flow dominating in the vicinity of the gas-liquid and solid-liquid interfaces while backpressure drives fluid in the opposite direction at the core of the film...
March 17, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28303845/diagnostic-value-of-nasopharyngeal-aspirates-in-children-with-lower-respiratory-tract-infections
#4
Ai-Zhen Lu, Peng Shi, Li-Bo Wang, Li-Ling Qian, Xiao-Bo Zhang
BACKGROUND: The accuracy of nasopharyngeal aspirate (NPA) specimens in detecting lower respiratory pathogens remains controversial. The objective of this study was to evaluate the diagnostic accuracy of aspirates (NPAs) specimen in lower respiratory tract infections (LRTIs) in children. METHODS: The prospective study was designed to collect the data of paired NPAs and bronchoalveolar lavage fluids from children with acute LRTIs from January 2013 to December 2015...
March 20, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28294224/creation-of-a-dual-porosity-and-dual-depth-micromodel-for-the-study-of-multiphase-flow-in-complex-porous-media
#5
Wonjin Yun, Cynthia M Ross, Sophie Roman, Anthony R Kovscek
Silicon-based microfluidic devices, so-called micromodels in this application, are particularly useful laboratory tools for the direct visualization of fluid flow revealing pore-scale mechanisms controlling flow and transport phenomena in natural porous media. Current microfluidic devices with uniform etched depths, however, are limited when representing complex geometries such as the multiple-scale pore sizes common in carbonate rocks. In this study, we successfully developed optimized sequential photolithography to etch micropores (1...
March 15, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28279117/progression-of-doppler-changes-in-early-onset-small-for-gestational-age-fetuses-how-frequent-are-the-different-progression-sequences
#6
José Morales-Roselló, Asma Khalil, Victoria Fornés-Ferrer, José Alberola-Rubio, David Hervas-Marín, Núria Peralta Llorens, Alfredo Perales-Marín
OBJECTIVE: To evaluate the progression of Doppler abnormalities in early-onset fetal smallness (SGA). METHODS: 948 Doppler examinations of the umbilical artery (UA), middle cerebral artery (MCA) and ductus venosus (DV), belonging to 405 early-onset SGA fetuses, were studied, evaluating the sequences of Doppler progression, the interval examination-labor at which Doppler became abnormal and the cumulative sum of Doppler anomalies in relation with labor proximity...
March 9, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28239372/identification-by-genome-mining-of-a-type-i-polyketide-gene-cluster-from-streptomyces-argillaceus-involved-in-the-biosynthesis-of-pyridine-and-piperidine-alkaloids-argimycins-p
#7
Suhui Ye, Brian Molloy, Alfredo F Braña, Daniel Zabala, Carlos Olano, Jesús Cortés, Francisco Morís, José A Salas, Carmen Méndez
Genome mining of the mithramycin producer Streptomyces argillaceus ATCC 12956 revealed 31 gene clusters for the biosynthesis of secondary metabolites, and allowed to predict the encoded products for 11 of these clusters. Cluster 18 (renamed cluster arp) corresponded to a type I polyketide gene cluster related to the previously described coelimycin P1 and streptazone gene clusters. The arp cluster consists of fourteen genes, including genes coding for putative regulatory proteins (a SARP-like transcriptional activator and a TetR-like transcriptional repressor), genes coding for structural proteins (three PKSs, one aminotransferase, two dehydrogenases, two cyclases, one imine reductase, a type II thioesterase, and a flavin reductase), and one gene coding for a hypothetical protein...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28209712/mycobacterium-tuberculosis-esxl-inhibit-mhc-ii-expression-by-promoting-hypermethylation-in-class-ii-transactivator-loci-in-macrophages
#8
Srabasti Sengupta, Saba Naz, Ishani Das, Abdul Ahad, Avinash Padhi, Sumanta Naik, Geetanjali Ganguli, Kaliprasad Patnaik, Sunil Kumar Raghav, Vinay Nandicoori, Avinash Sonawane
Mycobacterium tuberculosis (Mtb) is known to modulate the host immune responses to facilitate its persistence inside the host cells. One of the key mechanisms includes repression of class-II transactivator (CIITA) and MHC-II expression in infected macrophages. However, the precise mechanism of CIITA and MHC-II down-regulation is not well studied. Mtb 6-kDa early secretory antigenic target (ESAT-6) is a known potent virulence and antigenic determinant. Mtb genome encodes 23 such ESAT-6 family proteins. We herein report that Mtb and M...
February 16, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28205272/homometallic-dyiii-complexes-of-varying-nuclearity-from-2-to-21-synthesis-structure-and-magnetism
#9
Vadapalli Chandrasekhar, Sourav Biswas, Sourav Das, Joydev Acharya, Vierandra Kumar, Jan Leusen, Juan Manuel Herrera, Paul Koegerler, Enrique Colacio
The synthesis, structure and magnetic properties of the four Dy(III) coordination compounds isolated as [Dy2(LH2)2(μ2-η1:η1-Piv)]Cl·2MeOH·H2O (1), [Dy4(LH)2(μ3-OH)2(Piv)4(MeOH)2]·4MeOH·2H2O(2), [Dy6(LH2)3(tfa)3(O3PtBu)(Cl)3]Cl4·15.5H2O·4MeOH· 5CHCl3 (3) and [Dy21(L)7(LH)7(tfa)7]Cl7·15H2O·7MeOH·12CHCl3 (4) are reported (Piv = pivalate, tfa = 1,1,1-trifluoro-acetylacetone, O3PtBu = tert-butylphosphonate). Some of these polynuclear coordination complexes exhibit aesthetic and unprecedented structural features...
February 16, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28196656/a-cdna-clone-launched-platform-for-high-yield-production-of-inactivated-zika-vaccine
#10
Yujiao Yang, Chao Shan, Jing Zou, Antonio E Muruato, Diniz Nunes Bruno, Barbosa de Almeida Medeiros Daniele, Pedro F C Vasconcelos, Shannan L Rossi, Scott C Weaver, Xuping Xie, Pei-Yong Shi
A purified inactivated vaccine (PIV) using the Zika virus (ZIKV) Puerto Rico strain PRVABC59 showed efficacy in monkeys, and is currently in a phase I clinical trial. High-yield manufacture of this PIV is essential for its development and vaccine access. Here we report an infectious cDNA clone-launched platform to maximize its yield. A single NS1 protein substitution (K265E) was identified to increase ZIKV replication on Vero cells (a cell line approved for vaccine production) for both Cambodian FSS13025 and Puerto Rico PRVABC59 strains...
February 6, 2017: EBioMedicine
https://www.readbyqxmd.com/read/28196148/from-the-track-to-the-ocean-using-flow-control-to-improve-marine-bio-logging-tags-for-cetaceans
#11
Giovani Fiore, Erik Anderson, C Spencer Garborg, Mark Murray, Mark Johnson, Michael J Moore, Laurens Howle, K Alex Shorter
Bio-logging tags are an important tool for the study of cetaceans, but superficial tags inevitably increase hydrodynamic loading. Substantial forces can be generated by tags on fast-swimming animals, potentially affecting behavior and energetics or promoting early tag removal. Streamlined forms have been used to reduce loading, but these designs can accelerate flow over the top of the tag. This non-axisymmetric flow results in large lift forces (normal to the animal) that become the dominant force component at high speeds...
2017: PloS One
https://www.readbyqxmd.com/read/28186414/a-three-pronged-attack-to-investigate-the-electronic-structure-of-a-family-of-ferromagnetic-fe4ln2-cyclic-coordination-clusters-a-combined-magnetic-susceptibility-high-field-high-frequency-electron-paramagnetic-resonance-and-57-fe-m%C3%A3-ssbauer-study
#12
Sebastian F M Schmidt, Changhyun Koo, Valeriu Mereacre, Jaena Park, Dieter W Heermann, Vladislav Kataev, Christopher E Anson, Denis Prodius, Ghenadie Novitchi, Rüdiger Klingeler, Annie K Powell
We present the synthesis, structure, magnetic properties, as well as the Mössbauer and electron paramagnetic resonance studies of a ring-shaped [Fe(III)4Ln(III)2(Htea)4(μ-N3)4(N3)3(piv)3] (Ln = Y 1, Gd 2, Tb 3, Dy 4, Ho 5, Er, 6) coordination cluster. The Dy, Tb, and Ho analogues show blocking of the magnetization at low temperatures without applied fields. The anisotropy of the 3d ion and the exchange interaction between 3d and 4f ions in Fe4Ln2 complexes are unambiguously determined by high-field/high-frequency electron paramagnetic resonance measurements at low temperature...
February 10, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28169811/-improve-the-extravasation-assessment-of-peripheral-venous-catheter-by-nurses-with-the-establishment-of-a-standardized-instrument-and-suitable-for-children
#13
Nathalie Bochaton, Anne Sylvie Ramelet, Peter Rimensberger, Jean-Marie Januel
In pediatric and neonatal intensive care units, severity assessment of extravasation is difficult, considering the specificity of this population. The purpose of this study was to demonstrate the ability to improve the measurement of extravasation by nurses with the establishment of a standardized instrument and suitable for children. 66 nurses, randomly assigned to two groups, assessed the severity of extravasations using 15 clinical vignettes.The intervention group with the Pediatric Peripheral Intravenous Infiltration Scale (PIV Scale) (n=33) and the control group based on clinical judgment only (n=33)...
2016: Recherche en Soins Infirmiers
https://www.readbyqxmd.com/read/28159006/the-potential-influence-of-human-parainfluenza-viruses-detected-during-hospitalization-among-critically-ill-patients-in-kuwait-2013-2015
#14
Sahar Essa, Haya Al-Tawalah, Sarah AlShamali, Widad Al-Nakib
BACKGROUND: The four types of human parainfluenza viruses (PIV) are important causes of community-acquired pneumonia, particularly in children; however, limited information exists about the incidence of PIV in critically ill patients. The aim of this study is to describe the spectrum, incidence and clinical features of PIV-associated infections diagnosed during the hospital stay of patients admitted to pediatric intensive care unit (PICU) and intensive care unit (ICU) of 5 medical centers across Kuwait...
February 3, 2017: Virology Journal
https://www.readbyqxmd.com/read/28144057/respiratory-viruses-in-acute-exacerbations-of-chronic-obstructive-pulmonary-disease
#15
Parvaiz A Koul, Hyder Mir, Shabir Akram, Varsha Potdar, Mandeep S Chadha
OBJECTIVE: Acute exacerbations of chronic obstructive pulmonary disease (AECOPD) cause significant morbidity, mortality, and an inexorable decline of lung function. Data from developed countries have shown viruses to be important causes of AECOPD, but data from developing countries like India are scant. We set out to determine the contribution of viruses in the causation of hospitalized patients with AECOPD. METHODS: Twin nasopharyngeal/oropharyngeal swabs collected from 233 patients admitted with an acute AECOPD and tested for respiratory viruses including respiratory syncytial virus A and B, parainfluenza were (PIV) 1, 2, 3, and 4, human metapneumovirus (hMPV) A and B, influenza A and B, enterovirus, corona NL65, OC43, and 229E viruses, adenovirus 2 and 4, rhinovirus, and bocavirus, by duplex real time reverse-transcription polymerase chain reaction (qRT-PCR) using CDC approved primers and probes...
January 2017: Lung India: Official Organ of Indian Chest Society
https://www.readbyqxmd.com/read/28140436/en-route-intraosseous-access-performed-in-the-combat-setting
#16
Shelia Savell, Alejandra G Mora, Crystal A Perez, Vikhyat S Bebarta, Maj Joseph K Maddry
OBJECTIVE: To describe and compare vascular access practices used by en route care providers during medical evacuation (MEDEVAC). DESIGN: This was a retrospective cohort study. Medical records of US military personnel injured in combat and transported by MEDEVAC teams were queried. PATIENTS: The subjects were transported by military en route care providers, in the combat theater during Operation Enduring Freedom (OEF) between January 2011 and March 2014...
October 2016: American Journal of Disaster Medicine
https://www.readbyqxmd.com/read/28135577/piv-for-the-characterization-of-focused-field-induced-acoustic-streaming-seeding-particle-choice-evaluation
#17
Rafika Ben Haj Slama, Bruno Gilles, Maher Ben Chiekh, Jean-Christophe Béra
This research evaluates the use of Particle Image Velocimetry (PIV) technique for characterizing acoustic streaming flow generated by High Intensity Focused Ultrasound (HIFU). PIV qualification tests, focusing on the seeding particle size (diameter of 5, 20 and 50μm) were carried out in degassed water subjected to a focused field of 550kHz-frequency with an acoustic pressure amplitude of 5.2, 10.5 and 15.7bar at the focus. This study shows that the ultrasonic field, especially the radiation force, can strongly affect seeding particle behavior...
January 14, 2017: Ultrasonics
https://www.readbyqxmd.com/read/28117807/a-microfluidic-system-with-surface-patterning-for-investigating-cavitation-bubble-s-cell-interaction-and-the-resultant-bioeffects-at-the-single-cell-level
#18
Fenfang Li, Fang Yuan, Georgy Sankin, Chen Yang, Pei Zhong
In this manuscript, we first describe the fabrication protocol of a microfluidic chip, with gold dots and fibronectin-coated regions on the same glass substrate, that precisely controls the generation of tandem bubbles and individual cells patterned nearby with well-defined locations and shapes. We then demonstrate the generation of tandem bubbles by using two pulsed lasers illuminating a pair of gold dots with a few-microsecond time delay. We visualize the bubble-bubble interaction and jet formation by high-speed imaging and characterize the resultant flow field using particle image velocimetry (PIV)...
January 10, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28112331/-detection-of-parainfluenza-virus-in-immunocompromised-and-immunocompetent-patients-in-a-tertiary-hospital-in-sao-paulo-brazil
#19
Sheila N Parmezan, Clarice Camargo, Aripuanã Watanabe, Patricio Godoy-Martínez, Celso Granato, Nancy Bellei
Parainfluenza virus infections (PIV) were evaluated in patients with mild and severe infections through real time PCR. One thousand and sixty-seven samples were collected from subjects as follows: 233 adult renal transplanted outpatients, 129 children with congenital heart disease, 381 with adult hematopoietic stem cell patients and 324 hospitalized patients suspected of influenza A (H1N1) pdm09 infection. PIV was detected in 74 (6.9%) samples. VPI-3 was the most frequent (60.8%) and a higher risk was observed for older adults (p = 0...
October 2016: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
https://www.readbyqxmd.com/read/28102532/time-to-epinephrine-in-out-of-hospital-cardiac-arrest-a-retrospective-analysis-of-intraosseous-versus-intravenous-access
#20
Elliot M Ross, Julian Mapp, Chetan Kharod, David A Wampler, Christopher Velasquez, David A Miramontes
INTRODUCTION: The 2015 advanced cardiac life support update continues to advocate administering epinephrine during cardiac arrest. The goal of our study is to determine if prehospital intraosseous (IO) access results in shorter time to epinephrine than prehospital peripheral intravenous (PIV) access. METHODS: The out-of-hospital cardiac arrest (OHCA) database of a large, urban, fire-based emergency medical services system was searched for consecutive cases of OHCA between January 2013 and December 2015...
April 2016: American Journal of Disaster Medicine
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