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https://www.readbyqxmd.com/read/28409325/velocity-field-visualization-in-usp-dissolution-apparatus-3-using-particle-image-velocimetry
#1
Satish Perivilli, Richard Prevost, Erika Stippler
PURPOSE: The hydrodynamics in USP dissolution apparatus 3, at five different dip rates, was characterized by analyzing phase-averaged velocity fields obtained using Particle Image Velocimetry (PIV). METHODS: Phase locked 2 Component-PIV (2C-PIV) measurements were recorded on a typical dissolution apparatus 3 configuration with a black painted tablet fixed at the center of the bottom porous screen of the reciprocating cylinder. A trigger mechanism was employed to capture data over 12 phase positions for each reciprocation cycle...
April 13, 2017: Pharmaceutical Research
https://www.readbyqxmd.com/read/28393124/a-leishmania-murine-model-to-evaluate-the-immunomodulatory-properties-of-pythium-insidiosum-proteins
#2
Tatiana Maria Inêz-Ferreira, Leonel Mendoza, Raquel Vilela, Hélida Monteiro de Andrade, João Paulo Haddad, Fernanda Freire Campos Nunes, Isabela Moreira Gondim, Tânia Mara Pinto Dabés Guimarães, Vicente de Paulo Coelho Peixoto de Toledo
Pythium insidiosum immunomodulatory vaccine (PiV) has been tested in clinical and experimental pythiosis. Previous data showed that P. insidiosum immunogens have the ability to switch the Th2 immune response, normally in place during pythiosis, to a curative Th1 response. Pythiosis cannot be reproduced in experimental rodents with the exception of rabbits, and thus thorough evaluation of PiV´s immunomodulatory properties has been limited by the lack of a compatible inbred mouse model. In this study, we took advantage of the murine BALB/c Leishmania infection model, where infected mice produce a Th2 response, to evaluate the PiV Th2 to Th1 immunomodulatory potential...
March 2017: Heliyon
https://www.readbyqxmd.com/read/28379938/effects-of-cone-shaped-bend-inlet-cannulas-of-an-axial-blood-pump-on-thrombus-formation-an-experiment-and-simulation-study
#3
Guangmao Liu, Jianye Zhou, Hansong Sun, Yan Zhang, Haibo Chen, Shengshou Hu
BACKGROUND Cannula shape and connection style influence the risk of thrombus formation in the blood pump by varying the blood flow characteristics inside the pump. Inlet cannulas should be designed based on the need for anatomical fit and reducing the risk of thrombus generation in the blood pump. The effects on thrombus formation of the cone-shaped bend inlet cannulas of axial blood pumps should be studied. MATERIAL AND METHODS The cannulas were designed as cone-shaped, with 1 bent section connecting 2 straight sections...
April 5, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28374198/optimum-design-of-the-entrance-of-a-fishpond-laterally-to-the-main-stream-of-an-open-channel
#4
Yiannis Savvidis, Evangelos Keramaris, George Pechlivanidis, Christopher Koutitas
In this study, the optimum design of the entrance of a fishpond laterally to the main flow of an open channel was investigated numerically and experimentally. The flow characteristic measurements were realized with the PIV (particle image velocimetry) method. The mathematical simulations were based on the development of a two dimensional -mean in depthhydrodynamic model and a quasi three dimensional sediment transport model which includes processes of advection, diffusion, and settling of conservative suspended matter...
April 3, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28348044/model-assisted-measurements-of-suspension-feeding-flow-velocities
#5
Kevin T Du Clos, Ian T Jones, Tyler J Carrier, Damian C Brady, Peter A Jumars
Benthic marine suspension feeders provide an important link between benthic and pelagic ecosystems. The strength of this link is determined by suspension-feeding rates. Many studies have measured suspension-feeding rates using indirect clearance-rate methods, which are based on the depletion of suspended particles. Direct methods that measure the flow of water itself are less common, but they can be more broadly applied because, unlike indirect methods, direct methods are not affected by properties of the cleared particles...
March 27, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28341436/optimal-dosage-and-duration-of-pivmecillinam-treatment-for-uncomplicated-lower-urinary-tract-infections-a-systematic-review-and-meta-analysis
#6
REVIEW
Mariona Pinart, Jennifer Kranz, Katrin Jensen, Tanja Proctor, Kurt Naber, Frank Kunath, Florian Wagenlehner, Stefanie Schmidt
OBJECTIVE: To compare the efficacy and safety of different pivmecillinam (PIV) regimes for uncomplicated lower urinary tract infections (UTIs). METHODS: The MEDLINE, Embase, and Cochrane Library databases were searched. Randomized controlled clinical trials (RCTs) involving adults or children with symptoms suggestive of uncomplicated UTI and that compared different PIV regimes or PIV versus other antibiotics were included. Meta-analyses were conducted to obtain direct and indirect efficacy estimates...
March 21, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28337500/direct-observation-of-orientation-distributions-of-actin-filaments-in-a-solution-undergoing-shear-banding
#7
K Sato, I Kunita, Y Takikawa, D Takeuchi, Y Tanaka, T Nakagaki, H Orihara
Shear banding is frequently observed in complex fluids. However, the configuration of macromolecules in solutions undergoing shear banding has not yet been directly observed. In this study, by using the fact that F-actin solutions exhibit shear banding and actin filaments are visualized by fluorescent labels, we directly observed the intrinsic states of an actin solution undergoing shear banding. By combining the 3D imaging of labeled actin filaments and particle image velocimetry (PIV), we obtained orientation distributions of actin filaments in both high and low shear rate regions, whose quantitative differences are indicated...
March 24, 2017: Soft Matter
https://www.readbyqxmd.com/read/28332315/significant-increase-of-flow-kinetic-energy-in-nonresponders-patients-to-cardiac-resynchronization-therapy
#8
Sara Cimino, Dino Palombizio, Francesco Cicogna, Donatella Cantisani, Manuela Reali, Domenico Filomena, Valentina Petronilli, Carlo Iacoboni, Luciano Agati
BACKGROUNDS: It's still unclear if different patterns of intraventricular flow dynamics may be detected in patients nonresponders to cardiac resynchronization therapy (CRT) as compared to responders ones. Aim of this study was to evaluate the characteristics of left ventricular (LV) flow dynamics 6-months after CRT to identify Echo-particle imaging velocity (PIV) patterns were more frequently detected in nonresponders patients. METHODS: Thirty-two patients with dilated cardiomyopathy, undergoing CRT, were enrolled in this study...
March 23, 2017: Echocardiography
https://www.readbyqxmd.com/read/28329354/clinical-significance-of-human-coronavirus-in-bronchoalveolar-lavage-samples-from-hematopoietic-cell-transplantation-recipients-and-patients-with-hematologic-malignancies
#9
Chikara Ogimi, Alpana A Waghmare, Jane M Kuypers, Hu Xie, Cecilia C Yeung, Wendy M Leisenring, Sachiko Seo, Su-Mi Choi, Keith R Jerome, Janet A Englund, Michael Boeckh
Background: The possible role of human coronaviruses (HCoV) in lower respiratory tract disease (LRTD) in hematopoietic cell transplant (HCT) recipients and patients with hematologic malignancies (HM) has not been well studied. Methods: We conducted a retrospective review of HCT/HM patients with HCoV detected in bronchoalveolar lavage (BAL). HCoV strains were identified in BAL samples using strain-specific PCR. Mortality rates were compared among HCT recipients with LRTD caused by HCoV, respiratory syncytial virus (RSV), influenza or parainfluenza virus (PIV) by multivariable Cox regression analysis...
February 17, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28325304/improved-resistance-of-chemically-modified-nanocellulose-against-thermally-induced-depolymerization
#10
Melissa B Agustin, Fumiaki Nakatsubo, Hiroyuki Yano
The study demonstrated the improvement in the resistance of nanocellulose against thermally-induced depolymerization by esterification with benzoyl (BNZ) and pivaloyl (PIV). The change in the degree of polymerization (DP) and molecular weight distribution (MWD) after thermal treatment in nitrogen and in air was investigated using viscometry and gel permeation chromatography. BNZ and PIV nanocellulose esters without α-hydrogens gave higher DP and narrower MWD than pure bacterial cellulose; and the acetyl and myristoyl esters, which possess α-hydrogens...
May 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28314051/electroosmotic-flow-measurements-in-a-freely-suspended-liquid-film-experimhents-and-numerical-simulations
#11
Abdulkadir Hussein Sheik, Himiyage Chaminda Hemaka Bandulasena, Victor Starov, Anna Trybala
Fluid flow profiles in free liquid films stabilised by anionic and cationic surfactants under an external electric field were investigated. Depthwise velocity fields were measured at the mid region of the free liquid film by confocal micron-resolution particle image velocimetry and corresponding numerical simulations were performed using Finite Element Method to model the system. Depthwise change in velocity profiles was observed with electroosmotic flow dominating in the vicinity of the gas-liquid and solid-liquid interfaces while backpressure drives fluid in the opposite direction at the core of the film...
March 17, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28303845/diagnostic-value-of-nasopharyngeal-aspirates-in-children-with-lower-respiratory-tract-infections
#12
Ai-Zhen Lu, Peng Shi, Li-Bo Wang, Li-Ling Qian, Xiao-Bo Zhang
BACKGROUND: The accuracy of nasopharyngeal aspirate (NPA) specimens in detecting lower respiratory pathogens remains controversial. The objective of this study was to evaluate the diagnostic accuracy of aspirates (NPAs) specimen in lower respiratory tract infections (LRTIs) in children. METHODS: The prospective study was designed to collect the data of paired NPAs and bronchoalveolar lavage fluids from children with acute LRTIs from January 2013 to December 2015...
March 20, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28294224/creation-of-a-dual-porosity-and-dual-depth-micromodel-for-the-study-of-multiphase-flow-in-complex-porous-media
#13
Wonjin Yun, Cynthia M Ross, Sophie Roman, Anthony R Kovscek
Silicon-based microfluidic devices, so-called micromodels in this application, are particularly useful laboratory tools for the direct visualization of fluid flow revealing pore-scale mechanisms controlling flow and transport phenomena in natural porous media. Current microfluidic devices with uniform etched depths, however, are limited when representing complex geometries such as the multiple-scale pore sizes common in carbonate rocks. In this study, we successfully developed optimized sequential photolithography to etch micropores (1...
April 11, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28279117/progression-of-doppler-changes-in-early-onset-small-for-gestational-age-fetuses-how-frequent-are-the-different-progression-sequences
#14
José Morales-Roselló, Asma Khalil, Victoria Fornés-Ferrer, José Alberola-Rubio, David Hervas-Marín, Núria Peralta Llorens, Alfredo Perales-Marín
OBJECTIVE: To evaluate the progression of Doppler abnormalities in early-onset fetal smallness (SGA). METHODS: A total of 948 Doppler examinations of the umbilical artery (UA), middle cerebral artery (MCA) and ductus venosus (DV), belonging to 405 early-onset SGA fetuses, were studied, evaluating the sequences of Doppler progression, the interval examination-labor at which Doppler became abnormal and the cumulative sum of Doppler anomalies in relation with labor proximity...
March 27, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28239372/identification-by-genome-mining-of-a-type-i-polyketide-gene-cluster-from-streptomyces-argillaceus-involved-in-the-biosynthesis-of-pyridine-and-piperidine-alkaloids-argimycins-p
#15
Suhui Ye, Brian Molloy, Alfredo F Braña, Daniel Zabala, Carlos Olano, Jesús Cortés, Francisco Morís, José A Salas, Carmen Méndez
Genome mining of the mithramycin producer Streptomyces argillaceus ATCC 12956 revealed 31 gene clusters for the biosynthesis of secondary metabolites, and allowed to predict the encoded products for 11 of these clusters. Cluster 18 (renamed cluster arp) corresponded to a type I polyketide gene cluster related to the previously described coelimycin P1 and streptazone gene clusters. The arp cluster consists of fourteen genes, including genes coding for putative regulatory proteins (a SARP-like transcriptional activator and a TetR-like transcriptional repressor), genes coding for structural proteins (three PKSs, one aminotransferase, two dehydrogenases, two cyclases, one imine reductase, a type II thioesterase, and a flavin reductase), and one gene coding for a hypothetical protein...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28209712/mycobacterium-tuberculosis-esxl-inhibit-mhc-ii-expression-by-promoting-hypermethylation-in-class-ii-transactivator-loci-in-macrophages
#16
Srabasti Sengupta, Saba Naz, Ishani Das, Abdul Ahad, Avinash Padhi, Sumanta Naik, Geetanjali Ganguli, Kaliprasad Patnaik, Sunil Kumar Raghav, Vinay Nandicoori, Avinash Sonawane
Mycobacterium tuberculosis (Mtb) is known to modulate the host immune responses to facilitate its persistence inside the host cells. One of the key mechanisms includes repression of class-II transactivator (CIITA) and MHC-II expression in infected macrophages. However, the precise mechanism of CIITA and MHC-II down-regulation is not well studied. Mtb 6-kDa early secretory antigenic target (ESAT-6) is a known potent virulence and antigenic determinant. Mtb genome encodes 23 such ESAT-6 family proteins. We herein report that Mtb and M...
February 16, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28205272/homometallic-dy-iii-complexes-of-varying-nuclearity-from-2-to-21-synthesis-structure-and-magnetism
#17
Sourav Biswas, Sourav Das, Joydev Acharya, Vierandra Kumar, Jan van Leusen, Paul Kögerler, Juan Manuel Herrera, Enrique Colacio, Vadapalli Chandrasekhar
The synthesis, structure, and magnetic properties of four Dy(III) coordination compounds isolated as [Dy2 (LH2 )2 (μ2 -η(1) :η(1) -Piv)]Cl⋅2 MeOH⋅H2 O (1), [Dy4 (LH)2 (μ3 -OH)2 (Piv)4 (MeOH)2 ]⋅4 MeOH⋅2 H2 O (2), [Dy6 (LH2 )3 (tfa)3 (O3 PtBu)(Cl)3 ]Cl4 ⋅15.5 H2 O⋅4 MeOH⋅5 CHCl3 (3) and [Dy21 (L)7 (LH)7 (tfa)7 ]Cl7 ⋅15 H2 O⋅7 MeOH⋅12 CHCl3 (4) are reported (Piv=pivalate, tfa=1,1,1-trifluoroacetylacetone, O3 PtBu=tert-butylphosphonate). Among these, 3 displays an equilateral triangle topology with a side length of 9...
February 16, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28196656/a-cdna-clone-launched-platform-for-high-yield-production-of-inactivated-zika-vaccine
#18
Yujiao Yang, Chao Shan, Jing Zou, Antonio E Muruato, Diniz Nunes Bruno, Barbosa de Almeida Medeiros Daniele, Pedro F C Vasconcelos, Shannan L Rossi, Scott C Weaver, Xuping Xie, Pei-Yong Shi
A purified inactivated vaccine (PIV) using the Zika virus (ZIKV) Puerto Rico strain PRVABC59 showed efficacy in monkeys, and is currently in a phase I clinical trial. High-yield manufacture of this PIV is essential for its development and vaccine access. Here we report an infectious cDNA clone-launched platform to maximize its yield. A single NS1 protein substitution (K265E) was identified to increase ZIKV replication on Vero cells (a cell line approved for vaccine production) for both Cambodian FSS13025 and Puerto Rico PRVABC59 strains...
March 2017: EBioMedicine
https://www.readbyqxmd.com/read/28196148/from-the-track-to-the-ocean-using-flow-control-to-improve-marine-bio-logging-tags-for-cetaceans
#19
Giovani Fiore, Erik Anderson, C Spencer Garborg, Mark Murray, Mark Johnson, Michael J Moore, Laurens Howle, K Alex Shorter
Bio-logging tags are an important tool for the study of cetaceans, but superficial tags inevitably increase hydrodynamic loading. Substantial forces can be generated by tags on fast-swimming animals, potentially affecting behavior and energetics or promoting early tag removal. Streamlined forms have been used to reduce loading, but these designs can accelerate flow over the top of the tag. This non-axisymmetric flow results in large lift forces (normal to the animal) that become the dominant force component at high speeds...
2017: PloS One
https://www.readbyqxmd.com/read/28186414/a-three-pronged-attack-to-investigate-the-electronic-structure-of-a-family-of-ferromagnetic-fe4ln2-cyclic-coordination-clusters-a-combined-magnetic-susceptibility-high-field-high-frequency-electron-paramagnetic-resonance-and-57-fe-m%C3%A3-ssbauer-study
#20
Sebastian F M Schmidt, Changhyun Koo, Valeriu Mereacre, Jaena Park, Dieter W Heermann, Vladislav Kataev, Christopher E Anson, Denis Prodius, Ghenadie Novitchi, Rüdiger Klingeler, Annie K Powell
We present the synthesis, structure, magnetic properties, as well as the Mössbauer and electron paramagnetic resonance studies of a ring-shaped [Fe(III)4Ln(III)2(Htea)4(μ-N3)4(N3)3(piv)3] (Ln = Y 1, Gd 2, Tb 3, Dy 4, Ho 5, Er, 6) coordination cluster. The Dy, Tb, and Ho analogues show blocking of the magnetization at low temperatures without applied fields. The anisotropy of the 3d ion and the exchange interaction between 3d and 4f ions in Fe4Ln2 complexes are unambiguously determined by high-field/high-frequency electron paramagnetic resonance measurements at low temperature...
February 10, 2017: Inorganic Chemistry
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