Mia S Geromella, Chantal R Ryan, Jessica L Braun, Michael S Finch, Lucas A Maddalena, Olivia Bagshaw, Briana Hockey, Fereshteh Moradi, Rachel K Fenech, Jisook Ryoo, Daniel M Marko, Roopan Dhaliwal, Jake Sweezey-Munroe, Sophie I Hamstra, Georgina Gardner, Sebastian Silvera, Rene Vandenboom, Brian D Roy, Jeffrey A Stuart, Rebecca E K MacPherson, Val A Fajardo
Sarco(endo)plasmic reticulum Ca2+ -ATPase (SERCA) uncoupling in skeletal muscle, and mitochondrial uncoupling via uncoupling protein 1 (UCP1) in brown/beige adipose tissue are two primary mechanisms implicated in energy expenditure. Here, we investigated the effects of glycogen synthase kinase 3 (GSK3) inhibition via lithium chloride (LiCl) treatment on SERCA uncoupling in skeletal muscle and UCP1 expression in adipose. C2C12 and 3T3-L1 cells treated with LiCl had increased SERCA uncoupling and UCP1 protein levels, respectively, ultimately raising cellular respiration; however, this was only observed when LiCl treatment occurred throughout differentiation...
October 6, 2022: Journal of Biological Chemistry