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Baisuo Zhao, Weidong Lu, Shanshan Zhang, Kang Liu, Yanchun Yan, Jun Li
A Gram-stain-positive, cocci-shaped, non-spore-forming, and moderately halophilic bacterium, designed BZ-SZ-XJ29T, was isolated from a salt lake of China. On the basis of 16S rRNA gene sequence similarity, the most closely phylogenetic relatives were Bacillus saliphilus 6AGT (97.3 %) and other five Bacillus species (95.4-96.3 %). However, it shared only 89.5 % with Bacillus subtilis subsp. subtilis DSM 10T, indicating that this isolate might not be a member of Bacillus genus. The genomic DNA G+C content was 40...
December 29, 2016: International Journal of Systematic and Evolutionary Microbiology
Łukasz Jedynak, Maria Jedynak, Magdalena Kossykowska, Joanna Zagrodzka
An HPLC method with UV detection and separation with the use of a C30 reversed phase analytical column for the determination of chemical purity and assay of menaquinone-7 (MK7) in one chromatographic run was developed. The method is superior to the methods published in the USP Monograph in terms of selectivity, sensitivity and accuracy, as well as time, solvent and sample consumption. The developed methodology was applied to MK7 samples of active pharmaceutical ingredient (API) purity, MK7 samples of lower quality and crude MK7 samples before purification...
December 10, 2016: Journal of Pharmaceutical and Biomedical Analysis
Shih-Yao Lin, Asif Hameed, You-Cheng Liu, Yi-Han Hsu, Mei-Hua Hung, Wei-An Lai, Chiu-Chung Young
A novel amylolytic, nitrate-reducing and diazotrophic bacterium, designated strain CC-MHH0563T, isolated from a fermentor was assessed for its taxonomic status using polyphasic taxonomy. Cells of strain CC-MHH0563T was Gram-staining-negative, catalase- and oxidase-positive, mesophilic and aerobic cocci, which produced reddish nondiffusible-pigments. Growth was observed at 15-37 ºС (optimal 25 ºС), pH 6.0-8.0 (optimal pH 7.0) and salinity (0-3 %, w/v). Strain CC-MHH0563T showed highest pair-wise 16S rRNA gene sequence similarity to the members of genera Cerasicoccus (89...
December 12, 2016: International Journal of Systematic and Evolutionary Microbiology
Long Zhang, Xiao-Long Chen, Qiang Hu, Kai Chen, Xin Yan, Shun-Peng Li, Shiri Freilich, Jian-Dong Jiang
A novel Gram-stain-negative, non-spore-forming and short rod-shaped bacterium, strain LIP-5T, isolated from an activated sludge in a pesticide factory in Xinyi, China, was investigated for its taxonomic position by a polyphasic approach. Cell growth occurred at 16-42 °C (optimum, 30 °C), in the presence of 0-3.0 % (w/v) NaCl (optimum, without NaCl) and at pH 6.0-9.0 (optimum, pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the isolate was a member of the family Chitinophagaceae, with the closest relatives being Arachidicoccus rhizosphaerae Vu-144T (93...
November 2016: International Journal of Systematic and Evolutionary Microbiology
Myong Chol Kim, Chol Myong Kim, Ok Chol Kang, Yumin Zhang, Zuobing Liu, Danzeng Wangmu, Ziyan Wei, Yao Huang, Fang Peng
Strain K2-33028T, appeared brick-red colony on R2A plate, was isolated from marine sediment sample in Kings Bay, Svalbard Archipelago, Norway. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K2-33028T belonged to the genus Hymenobacter. Cells were Gram-reaction-negative, non-spore-forming, aerobic, rod-shaped bacterium without motility. Growth occurred at 4-37℃ (optimum 28℃) and at pH 6.0-8.0 (optimum pH 7.0). Cells contained menaquinone-7, as the main respiratory quinone, and iso-C15:0, summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c), C16:1 ω5c, summed feature 4 (comprising anteiso-C17:1 B and/or iso-C17:1 I) and anteiso-C15:0 as the major cellular fatty acids...
November 21, 2016: International Journal of Systematic and Evolutionary Microbiology
Huiqin Huang, Fute Zhang, Min Liu, Ying Cui, Qianguang Sun, Jun Zhu, Xiaoxiao Zou, Shixiang Bao
Two Gram-stain-positive, facultatively aerobic, endospore-forming and rod-shaped bacteria, designated DB13031T and DB13311, were isolated from soil of Jiaxi Nature Reserve in Hainan, China. 16S rRNA gene analysis of the strains DB13031T and DB13311 showed that they were within the Paenibacillus cluster, with the highest similarities of Paenibacillus cucumis AP-115T (98.4 % and 98.3 %, respectively), Paenibacillus barcinonensis BP-23T (98.3 % and 98.2 %, respectively) and Paenibacillus oceanisediminis L10T (97...
October 25, 2016: International Journal of Systematic and Evolutionary Microbiology
Myong Chol Kim, Ok Chol Kang, Chol Myong Kim, Yumin Zhang, Zuobing Liu, Ziyan Wei, Yao Huang, Wangmu Danzeng, Fang Peng
A yellow-pigmented bacteria strain, designated M1-33108T, was isolated from the till of high Arctic glacier Midtre Lovénbreen (MLB) near Ny-Ålesund, in the West Svalbard Archipelago, Norway. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M1-33108T belonged to the genus Terrimonas and its closest neighbor was Terrimonas arctica R9-86T with 96.12% 16S rRNA gene sequence similarity. Strain M1-33108T was Gram-reaction-negative, aerobic, non-spore-forming, rod-shaped bacterium that lacked motility...
November 24, 2016: International Journal of Systematic and Evolutionary Microbiology
Jun-Lian Gao, Pengbo Sun, Xiao-Jie Mao, Yan-Li Du, Bing-Yan Liu, Jian-Guang Sun
A novel Gram-staining-negative, aerobic, non-motile by gliding and rod-shaped strain designated 22T was isolated from surface-sterilized root tissue of maize planted in the Fangshan District of Beijing, People's Republic of China. The highest levels of 16S rRNA gene sequence similarity were found with respect to Pedobacter suwonensis15-52T (97.5 %), Pedobacter terrae DS-57T (97.1 %) and Pedobacter alluvionis NWER-II11T (97.0 %). Phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain 22T is a member of the genus Pedobacter...
October 25, 2016: International Journal of Systematic and Evolutionary Microbiology
Song-Ih Han, Ye-Rim Lee, Ju-Ok Kim, Kyung-Sook Whang
The novel isolate belonging to the genus Terrimonas, designated CR94T, was isolated from rhizosphere soil of ginseng field in Geumsan in Korea. Cells of Strain CR94T were strictly aerobic, Gram staining negative, non-motile, non-filamentous single rods. Growth was observed at 10-37℃ (optimum 28℃) and at pH4.0-10.0 (optimum pH6.0). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain CR94T belonged to the genus Terrimonas, showing the highest sequence similarity to Terrimonas lutea DYT (97...
November 2, 2016: International Journal of Systematic and Evolutionary Microbiology
Shih-Yao Lin, Asif Hameed, Kai-Yeh Chiu, You-Cheng Liu, Yi-Han Hsu, Wei-An Lai, Chiu-Chung Young
A polyphasic approach was used to characterize a presumably novel bacterium, designated strain CC-KYC063T, isolated from a compost sample collected at a greenhouse facility within Taiwan. Strain CC-KYC063T was Gram-staining-negative and aerobic, able to grow at 15-37 ºС (optimal 30 ºС), pH 7.0-9.0 (optimal pH 7.0) and salinity (0-2 %, w/v). The 16S rRNA gene sequence analysis of strain CC-KYC063T showed highest sequence similarity to Olivibacter ginsengisoli Gsoil 060T (96.2 %) and Olivibacter terrae Jip13T (95...
October 21, 2016: International Journal of Systematic and Evolutionary Microbiology
Eun Sun Joo, Eun Bit Kim, Seon Hwa Jeon, Sathiyaraj Srinivasan, Myung Kyum Kim
Strain JBM2-3T, a pale-yellow-colored, aerobic, catalase-negative, oxidase-positive, and Gram-negative bacterium, was isolated from wet soil. The isolate grew aerobically at 25-30°C (optimum 25°C), pH 6.0-8.0 (optimum pH 7.0) and in the presence of 0-0.5 % (w/v) NaCl (optimum 0 % NaCl). Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain JBM2-3T belonged to the genus Spirosoma with a sequence similarity of 96.2% with Spirosoma panaciterrae Gsoil 1519T. The strain showed the typical chemotaxonomic characteristics of the genus Spirosoma, with the presence of respiratory quinone as menaquinone 7; the major fatty acids are summed feature 3 (composed of C16:1 ω6c/ω7c), C16:1ω5c, and C15:0 iso...
October 21, 2016: International Journal of Systematic and Evolutionary Microbiology
Hong-Fei Wang, Qiu-Li Li, Yong-Guang Zhang, Min Xiao, Xing-Kui Zhou, Jian-Wei Guo, Yan-Qing Duan, Wen-Jun Li
A novel endophytic bacterium, designated strain EGI 6500252T, was isolated from the surface-sterilized roots of medicinal plant (Capparis spinosa L.) collected from Urumqi city, Xinjiang, north-west of China. Cells were Gram-staining-positive, non-motile, aerobic, catalase- and oxidase-positive, rod-shaped and did not display spore formation. Strain EGI 6500252T grew at 10-40 °C (optimum 25-30 °C) and pH 6.0-8.0 (optimum pH 7.0), and in the presence of 0-10 % NaCl (w/v) (optimum 0-3 %). The major cellular fatty acids (> 10 %) were identified as iso-C15:0, anteiso-C15:0, anteiso-C17:0 and Sum In feature 4...
October 26, 2016: International Journal of Systematic and Evolutionary Microbiology
Jinlong Song, Yanwei Wang, Yi Song, Bingqiang Zhao, Huimin Wang, Shan Zhou, Delong Kong, Xiang Guo, Yanting Li, Mingxiong He, Kedong Ma, Zhiyong Ruan, Yanchun Yan
Two novel aerobic bacteria, designated strains LAM0312T and LAM0313, were isolated from saline soil sample collected from paddy field in Dezhou city, Shandong Province in China. The Cells of these strains were Gram-stain positive, sporogenous, rod-shaped and motile with peritrichous flagella. The optimal growth temperature and pH were 30 ºC and pH 7.0-8.0, repesctively. Strain LAM0312T was found to be able to grow in the presence of 12 % (w/v) NaCl. The major fatty acids of strains were identified as anteiso-C15:0 and iso-C15:0...
October 17, 2016: International Journal of Systematic and Evolutionary Microbiology
Ji-Quan Sun, Lian Xu, Xiao-Lei Wu
A Gram-staining-positive, aerobic bacterial strain, designated Y2A20T with peritrichous flagella was isolated from the top layer saline-alkaline soil, Hangjin Banner, Inner Mongolia, northern China. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain Y2A20T formed a stable clade with 'Lysinibacillus jejuensis' N2-5T. Strain Y2A20T shared the highest 16S rRNA gene sequence similarity with 'L. jejuensis' N2-5T (97.4%), while lower 16S rRNA gene similarities with all other type strains (<97...
October 12, 2016: International Journal of Systematic and Evolutionary Microbiology
Jae-Bong Lee, Seon Hwa Jeon, Seok-Gwan Choi, Hee-Young Jung, Myung Kyum Kim, Sathiyaraj Srinivasan
In this paper, a new bacterial strain designated as 16MFT21(T) is isolated from the muscle of a fish caught in the Antarctic Ocean. Strain 16MFT21(T) is a Gram-staining-positive, catalase-oxidase-positive, rod-shaped facultative-aerobic bacterium. The phylogenetic analysis that is based on the 16S-rRNA gene sequence of strain 16MFT21(T) revealed that it belongs to the genus Bacillus in the family Bacillaceae in the class Bacilli. The highest degrees of the sequence similarity of the strain 16MFT21(T) is with Bacillus licheniformis ATCC 14580(T) (96...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
Ehsan Mahdinia, Ali Demirci, Aydin Berenjian
Menaquinone-7, a highly valuable member of the vitamin K series, has significant effects on preventing osteoporosis and cardiovascular disease besides its positive effects on blood coagulation. In this review, chemical and biological aspects of menaquinone-7 are briefly summarized followed by a critical review on upstream and downstream processing developments for its production and recovery, including solid versus liquid fermentations, static versus agitated fermentations and online versus post-production recovery...
January 2017: World Journal of Microbiology & Biotechnology
Priyanka Singh, Hina Singh, Yeon-Ju Kim, Deok-Chun Yang
A novel strain, DCY108(T) was isolated from soil of a Panax ginseng field, Yeoncheon province (38°04'N 126°57'E), Republic of Korea. Strain DCY108(T) is Gram-negative, non-motile, non-flagellate, rod-shaped, and aerobic. The bacterium grows optimally at 25-30 °C, pH 6.5-7.0 and 1 % NaCl. Phylogenetically, strain DCY108(T) is closely related to Pedobacter jejuensis JCM 18824(T), Pedobacter aquatilis JCM 13454(T), Pedobacter kyungheensis LMG 26577(T) and the type strain of the genus Pedobacter heparinus DSM 2366(T)...
October 31, 2016: Antonie Van Leeuwenhoek
Hani M Khojah, Sameh Ahmed, Mahran S Abdel-Rahman, Khaled M Alkhalil, Al-Badr Hamza
The aim of this study was to evaluate the possible role of vitamin K homologs as potential biomarkers for disease activity in patients with rheumatoid arthritis (RA). In this study, 42 patients with RA and 40 healthy controls were enrolled. Serum levels of vitamin K homologs were measured using a high-performance liquid chromatography-fluorescence method. Different biochemical and clinical markers for disease activity were measured and correlated with serum levels of vitamin K homologs. There were no significant differences between RA patients and healthy subjects in demographic data...
October 8, 2016: Journal of Bone and Mineral Metabolism
Zheng-Fei Yan, Pei Lin, Yu-Shuai Wang, Wei Gao, Chang-Tian Li, Moo-Chang Kook, Tae-Hoo Yi
A Gram-negative, aerobic, non-motile, long rods with no flagellum strain, designated THG-YS3.2.1T, was isolated from rhizosphere soil of mugunghwa, collected from Kyung Hee University, Yongin, South Korea. Growth occurred at 10-40 oC (optimum 28 oC), at pH 6.0-8.0 (optimum 7.0) and at 0-1.0% NaCl (optimum 1.0%). The predominant menaquinone was menaquinone-7 (MK-7). The major cellular fatty acids were anteiso-C12:0, iso-C14:0, iso-C15:0, anteiso-C15:0, iso-C15:1 G, anteiso-C15:1 A, C15:0 2OH, C16:0, iso-C16:0, C16:0 3OH, iso-C16:0 3OH, iso-C16:1 G, C17:0 2OH, iso-C17:0 3OH, C17:1 ω6c, C18:3 ω6c (6,9,12), C18:0, summed feature 3 (C16:1 ω7c and/or iso-C15:0 2OH)...
September 13, 2016: International Journal of Systematic and Evolutionary Microbiology
Sofie Hertz Rønn, Torben Harsløf, Steen Bønløkke Pedersen, Bente Lomholt Langdahl
OBJECTIVE: Clinical studies suggest that vitamin K2 protects against bone loss and fractures; however, its effect on bone quality has never been investigated. We investigated the effect of vitamin MK-7 on undercarboxylated osteocalcin (ucOC), and bone mass and quality. DESIGN: We conducted a randomised, placebo-controlled, double-blinded clinical trial. METHODS: We investigated the effect of MK-7 375 µg for 12 months on bone mineral density (BMD) measured by dual X-ray absorptiometry (DXA), bone microarchitecture measured by high-resolution peripheral quantitative computed tomography (HRpQCT) and biochemical bone turnover markers in 148 postmenopausal women with osteopenia...
December 2016: European Journal of Endocrinology
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