Read by QxMD icon Read


Arkadiusz Szterk, Adam Zmysłowski, Katarzyna Bus
For the first time, the cis/trans isomer content of menaquinone-7 in food products has been identified and marked. A novel method of marking isomers of vitamin K2MK-7 in dietary supplements was developed and validated. Five different isomers of cis/trans vitamin K2MK-7 were identified. Identification of cis/trans isomers was performed by HRMS-QTOF, whereas their quantities were determined by using CAD and DAD detectors. In the majority of cases, the content of biologically active all trans vitamin K2MK-7 was below its declared content...
March 15, 2018: Food Chemistry
Dhiraj Kumar Chaudhary, Ram Hari Dahal, Jaisoo Kim
Two novel strains, J116-2(T) and J116-1, were isolated from forest soil and were taxonomically characterized by a polyphasic approach. Both strains were yellow-coloured, Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacteria. The strains were non-sporulating, catalase-positive and oxidase-negative. Strains J116-2(T) and J116-1 were able to grow at 20-32 °C, pH 6.0-8.5, and 0-0.5 % (w/v) NaCl concentration. Based on 16S rRNA gene sequence analyses, strains J116-2(T) and J116-1 formed a distinct lineage within the family Chitinophagaceae of the phylum Bacteroidetes and were closely related to genera Taibaiella (89...
November 15, 2017: International Journal of Systematic and Evolutionary Microbiology
Soon Youl Lee, Muhammad Zubair Siddiqi, Sang Yong Kim, Hong Shan Yu, Jae Hak Lee, Wan-Taek Im
A Gram-reaction-negative, strictly aerobic, non-motile and rod-shaped bacterium, designated strain BXN5-31(T), was isolated from soil of a ginseng field, and its taxonomic position was investigated using a polyphasic approach. Strain BXN5-31(T) grew at 18-37 °C and at pH 6.0-8.0 on R2A medium. Based on 16S rRNA gene sequence similarity, strain BXN5-31(T) was shown to belong to the genus Mucilaginibacter and was closely related to Mucilaginibactersoyangensis HME6664(T), Mucilaginibacterximonensis XM-003(T) and Mucilaginibacterpuniceus WS71(T)...
November 14, 2017: International Journal of Systematic and Evolutionary Microbiology
Ehsan Mahdinia, Ali Demirci, Aydin Berenjian
Menaquinone-7 (MK-7) is the key form of vitamin K used as a dietary supplement and its production revolves around Bacillus subtilis natto. Current fermentation strategies, which suggest static fermentations without aeration and agitation, can be problematic for large scale MK-7 production due to biofilm formation. The use of biofilm reactors, therefore, is proposed in the present study, which could utilize both agitation and aeration without interrupting MK-7 secretion. In this study, biofilm reactors were constructed using the selected plastic composite support (PCS) and B...
November 8, 2017: Bioprocess and Biosystems Engineering
Dinali Ranmadugala, Alireza Ebrahiminezhad, Merilyn Manley-Harris, Younes Ghasemi, Aydin Berenjian
One of the major issues associated with industrial production of menaquinone-7 (MK-7) is the low fermentation yield. In this study, we investigated the effect of iron oxide nanoparticles coated with 3-aminopropyltriethoxysilane (IONs@APTES) on the production of MK-7 using B. subtilis (ATCC 6633). Decoration of B. subtilis cells with IONs@APTES significantly enhanced both MK-7 production and yield. An approximately two-fold increase in MK-7 production (41 mg/L) was observed in the presence of 500 µg/mL IONs@APTES, as compared to MK-7 production using untreated bacteria (22 mg/L)...
October 26, 2017: Nanomaterials
Su-Kyoung Shin, Eunji Kim, Hana Yi
A Gram-stain-positive, endospore-forming, rod-shaped, aerobic bacterium, designated LPB0068(T), was isolated from a Pacific oyster (Crassostrea gigas) in Korea. This isolate was found to share the highest 16S rRNA gene sequence similarity with Paenibacillus macquariensis subsp. macquariensis DSM 2(T) (98.1 %) and Paenibacillus macquariensis subsp. defensor JCM 14954(T) (98.0 %). To establish the genomic relatedness of this isolate to its phylogenetic neighbours, its genome sequence and those of Paenibacillus antarcticus CECT 5836(T), P...
October 25, 2017: International Journal of Systematic and Evolutionary Microbiology
Fangfang Xu, Yu Tao, Heng Li, Jianyu Meng, Ziyi Zhang, Fuying Feng
A novel bacterial strain, designated MIMbqt9(T), was isolated from biological soil crusts collected in Erdos Plateau in Inner Mongolia. Cells were light pink, rod-shaped, aerobic, Gram-reaction-negative, motile by gliding, oxidase- and catalase-positive. Cell growth could be observed at 5-40 °C (optimum at 30 °C), at pH 4.0-6.0 (optimum at 6.0) and with 1 % (w/v) NaCl. Phylogenetic analysis of 16S rRNA gene sequence showed that strain MIMbqt9(T) was most closely related to the members of genus Larkinella, including L...
October 23, 2017: International Journal of Systematic and Evolutionary Microbiology
Dhiraj Kumar Chaudhary, Sang Don Lee, Jaisoo Kim
Strain K-4-11-1(T), a psychrotolerant, light salmon-coloured, Gram-stain-negative, non-motile and rod-shaped bacterium, was isolated from forest soil of Kyonggi University, Suwon, South Korea. It was able to grow at 0-32 °C, at pH 5.0-10.0 and with 0-1.5 % (w/v) NaCl. This strain was taxonomically characterized by a polyphasic approach. Based on 16S rRNA gene sequence analysis, strain K-4-11-1(T) belongs to the genus Pedobacter and is closely related to Pedobacter ginsenosidimutans THG-45(T) (98.75 % sequence similarity), Pedobacter borealis G-1(T) (98...
October 19, 2017: International Journal of Systematic and Evolutionary Microbiology
Jingran Huang, Zhaobin Huang, Zongze Shao
Two strains, AFRC7-2-1(T) and CFD7-4-2, were isolated from the intestine of abalone, Haliotis discus hannai. The bacterial cells were Gram-stain-negative, stick-shaped and non-flagellated. They were facultative anaerobic bacteria and could reduce Fe(III) to Fe(II). The 16S rRNA genes of two strains shared 99.7 % sequence similarity, and had the highest similarity of 97.3-97.5 % with Paraferrimonas sedimenticola NBRC 101628(T), and of <94.0 % with other species. Phylogenetic relationship showed that the two strains formed a tight clade with an isolate Paraferrimonas sp...
October 17, 2017: International Journal of Systematic and Evolutionary Microbiology
Leonid N Ten, Jongguen Jeon, Su-Jin Park, Sangkyu Park, Seung-Yeol Lee, Myung Kyum Kim, Hee-Young Jung
A Gram-stain negative, non-motile, rod-shaped, aerobic bacterium, designated 15J8-8(T), was isolated from a soil sample collected on Jeju Island, South Korea, and characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 15J8-8(T) belongs to the family Cytophagaceae and is related to Larkinella bovis M2TB15(T) (95.0%), 'Larkinella harenae' 15J9-9 (94.5%), Larkinella arboricola Z0532(T) (93.2%), and Larkinella insperata LMG 22510(T) (93.0%). The DNA G+C content of strain 15J8-8(T) was 50...
October 10, 2017: Antonie Van Leeuwenhoek
Lan Yang, Yun-Hao Wang, Hai-Zhen Zhu, Jiang-Baota Muhadesi, Bao-Jun Wang, Shuang-Jiang Liu, Cheng-Ying Jiang
Strain WY-1(T), a Gram-stain-negative, non-spore-forming, rod-shaped, non-motile bacterium, was isolated from the sewage treatment packing of a coking chemical plant. Strain WY-1(T) grew over a temperature range of 15-45 °C (optimum, 30-37 °C), a pH range of 5.5-11.0 (optimum, pH 6.5-7.0) and an NaCl concentration range of 0-3 % (w/v; optimum, 0 %). 16S rRNA gene sequence analysis showed that strain WY-1(T)was closely related to Parapedobacter indicus RK1(T) with the highest sequence similarity of 96...
October 6, 2017: International Journal of Systematic and Evolutionary Microbiology
Yunho Lee, Che Ok Jeon
A Gram-stain-positive, facultatively aerobic and endospore-forming bacterium, designated strain Pch-40(T), was isolated from a freshwater green alga, Paulinella chromatophora. Cells were motile rods with a monotrichous polar flagellum showing catalase- and oxidase-positive reactions. Strain Pch-40(T) grew at 20-50 °C (optimum, 37-40 °C), at pH 5.0-11.0 (optimum, pH 7.0) and in the presence of 0-4.0 % (w/v) NaCl (optimum, 0 %). Menaquinone-7 was detected as the sole isoprenoid quinone. The genomic DNA G+C content of strain Pch-40(T) was 55...
October 6, 2017: International Journal of Systematic and Evolutionary Microbiology
Hai-Zhen Zhu, Lan Yang, Jiang-Baota Muhadesi, Bao-Jun Wang, Shuang-Jiang Liu
A novel Gram-stain-negative, rod-shaped, non-motile and red-pigmented bacterial strain, designated K1E01-27(T), was isolated from an animal excrement sample which was found in a karst cave located in Guizhou province, China. The phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K1E01-27(T) represented a member of the genus Hymenobacter within the family Cytophagaceae of the phylum Bacteroidetes. Strain K1E01-27(T) was most closely related to Hymenobacter algoricola VUG-A23a(T), with 95...
October 6, 2017: International Journal of Systematic and Evolutionary Microbiology
Dong Jin Yang, Jin-Kyung Hong
The bacteria strain EN12(T) was isolated from forest soil in the Republic of Korea. The cells were Gram-negative, non-motile and rod-shaped, and the strain was strictly aerobic. Phylogenetic analysis of its 16S rRNA gene sequences showed that strain EN12(T) belonged to the class Sphingobacteriia of the phylum Bacteroidetes, and its closest relative is Pedobacter namyangjuensis 5G38(T), with a sequence similarity of 95.5 %. The average DNA sequence similarity from validly described species within the genus Pedobacter was 92...
October 6, 2017: International Journal of Systematic and Evolutionary Microbiology
M H J Knapen, K M Jardon, C Vermeer
BACKGROUND/OBJECTIVES: Vitamin K status has been linked to fat and glucose metabolism by several authors, but whether high vitamin K intake influences body weight or composition has remained unclear. Here we tested the hypothesis that increased vitamin K intake decreases body fat or fat distribution. SUBJECTS/METHODS: In a randomized placebo-controlled human intervention trial, 214 postmenopausal women, 55-65 years of age, received either 180 mcg/day of vitamin K2 (menaquinone-7, MK-7) or placebo for 3 years...
September 27, 2017: European Journal of Clinical Nutrition
Hubert Antolak, Joanna Oracz, Anna Otlewska, Dorota Żyżelewicz, Dorota Kręgiel
The aim of the study was to identify and quantitatively assess of carotenoids and isoprenoid quinones biosynthesized by six different strains of acetic acid bacteria, belonging to genus Asaia, that are common beverage-spoiling bacteria in Europe. Bacterial cultures were conducted in a laboratory liquid culture minimal medium with 2% sucrose. Carotenoids and isoprenoid quinones were investigated using UHPLC-DAD-ESI-MS analysis. In general, tested strains of Asaia spp. were able to produce 10 carotenoids and 3 isoprenoid quinones: menaquinone-7, menaquinone-8, and ubiquinone-10...
September 25, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Jae-Chan Lee, Kyung-Sook Whang
A Gram-stain-positive, halotolerant bacterium, designated strain BH128T, was isolated from soil of a saltern located at Bigeum Island in south-west Korea. Cells were aerobic, motile, spore-forming rods and grew at 15-53 °C (optimum, 35 °C), at pH 5.5-9.0 (optimum, pH 7.0) and at salinities of 0-16 % (w/v) NaCl (optimum, 8 % NaCl). The predominant isoprenoid quinone was menaquinone-7 (MK-7), and the cell-wall peptidoglycan type was A1γ, with meso-diaminopimelic acid as the diagnostic diamino acid...
September 25, 2017: International Journal of Systematic and Evolutionary Microbiology
Yunhee Lee, Hyun Mi Jin, Hye Su Jung, Che Ok Jeon
A Gram-stain-negative, facultatively aerobic bacterium, designated strain D1T, was isolated from soil in South Korea. Cells of strain D1T were non-motile rods with oxidase- and catalase-positive activities. Growth was observed at 15-40 °C (optimum, 30-37 °C), at pH 5.5-9.0 (optimum, pH 7.0-8.0) and in the presence of 0.0-5.0 % (w/v) NaCl (optimum, 0.0-1.0 %). The only respiratory quinone detected was menaquinone 7 (MK-7), and iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (comprising C16 : 1ω7c/C16 : 1ω6c) were identified as the major fatty acids...
September 25, 2017: International Journal of Systematic and Evolutionary Microbiology
Min Cao, Jing Huang, Jingxin Li, Zixu Qiao, Gejiao Wang
A yellow-coloured, Gram-stain-negative, non-motile, rod-shaped, strictly aerobic bacterium, designated 1-116T, was isolated from Erdos grassland soil of Inner Mongolia, PR China. Phylogenetic analysis based on 16S rRNA genes showed that strain 1-116T was a member of family Chitinophagaceae and exhibited the highest similarities to Taibaiella koreensis THG-DT86T (90.1 %) and Flavihumibacter solisivae 3-3T (90.0 %), while the similarities to the other Chitinophagaceae type strains were lower than 90.0 %...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
In-Tae Cha, Eui-Sang Cho, Yesol Yoo, Yoon Ji Seok, Inhye Park, Hee Seon Lim, Jung-Min Park, Seong Woon Roh, Young-Do Nam, Hak-Jong Choi, Yoo Kyung Lee, Myung-Ji Seo
A Gram-positive, endospore-forming, strictly aerobic and rod-shaped bacterium, designated strain MME2_R6T, was isolated from Arctic soil, and it was identified by using a polyphasic taxonomic approach. This strain was psychrotolerant, growing at 4‒24 °C. 16S rRNA gene sequence analysis showed that strain MME2_R6T was closest to Paenibacillus swuensis DY6T, with 93.9 % similarity. However, in phylogenetic analysis based on the 16S rRNA gene sequence, strain MME2_R6T showed that it clustered with Paenibacillus contaminans CKOBP-6T and the sequencing similarity between the two species was 93...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"