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https://www.readbyqxmd.com/read/28317856/a-smartphone-based-diagnostic-platform-for-rapid-detection-of-zika-chikungunya-and-dengue-viruses
#1
Aashish Priye, Sara W Bird, Yooli K Light, Cameron S Ball, Oscar A Negrete, Robert J Meagher
Current multiplexed diagnostics for Zika, dengue, and chikungunya viruses are situated outside the intersection of affordability, high performance, and suitability for use at the point-of-care in resource-limited settings. Consequently, insufficient diagnostic capabilities are a key limitation facing current Zika outbreak management strategies. Here we demonstrate highly sensitive and specific detection of Zika, chikungunya, and dengue viruses by coupling reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with our recently developed quenching of unincorporated amplification signal reporters (QUASR) technique...
March 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28316881/occurrence-of-concurrent-infections-with-multiple-serotypes-of-dengue-viruses-during-2013-2015-in-northern-kerala-india
#2
Manchala Nageswar Reddy, Ranjeet Dungdung, Lathika Valliyott, Rajendra Pilankatta
BACKGROUND: Dengue is a global human public health threat, causing severe morbidity and mortality. The occurrence of sequential infection by more than one serotype of dengue virus (DENV) is a major contributing factor for the induction of Dengue Hemorrhagic Fever (DHF) and Dengue Shock Syndrome (DSS), two major medical conditions caused by DENV infection. However, there is no specific drug or vaccine available against dengue infection. There are reports indicating the increased incidence of concurrent infection of dengue in several tropical and subtropical regions...
2017: PeerJ
https://www.readbyqxmd.com/read/28295400/paper-based-point-of-care-testing-for-cost-effective-diagnosis-of-acute-flavivirus-infections
#3
F Bedin, L Boulet, E Voilin, G Theillet, A Rubens, C Rozand
Flavivirus infections are a serious healthcare concern in tropical and subtropical countries. Although well-established laboratory tests can provide early diagnosis of acute dengue or Zika infections, access to these tests is limited in developing countries, presenting an urgent need to develop simple, rapid, and robust diagnostic tools. Microfluidic Paper-based Analytical Devices (µPAD), are typically rapid, cost-effective, user-friendly, and they can be used as diagnostic tools for the diagnosis of these infections at Point of Care settings...
March 11, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28293273/mathematical-modelling-of-immune-parameters-in-the-evolution-of-severe-dengue
#4
M K Premaratne, S S N Perera, G N Malavige, Saroj Jayasinghe
Aims. Predicting the risk of severity at an early stage in an individual patient will be invaluable in preventing morbidity and mortality caused by dengue. We hypothesized that such predictions are possible by analyzing multiple parameters using mathematical modeling. Methodology. Data from 11 adult patients with dengue fever (DF) and 25 patients with dengue hemorrhagic fever (DHF) were analyzed. Multivariate statistical analysis was performed to study the characteristics and interactions of parameters using dengue NS1 antigen levels, dengue IgG antibody levels, platelet counts, and lymphocyte counts...
2017: Computational and Mathematical Methods in Medicine
https://www.readbyqxmd.com/read/28292653/co-circulating-serotypes-in-a-dengue-fever-outbreak-differential-hematological-profiles-and-phylogenetic-relationships-among-viruses
#5
Andreia Moreira Dos Santos Carmo, Rodrigo Buzinaro Suzuki, Aline Diniz Cabral, Renata Torres da Costa, Gabriela Pena Massari, Michele Marcondes Riquena, Helio Augusto Alves Fracasso, Andre Eterovic, Arlei Marcili, Márcia Aparecida Sperança
BACKGROUND: Dengue virus, represented by four distinct, genetically diverse serotypes, is the etiologic agent of asymptomatic to severe hemorrhagic diseases. The spatiotemporal dynamics of dengue serotypes and its association to specific diseases vary among the different regions worldwide. By 2007, and in São Paulo State, Brazil, dengue-case concentration in urban centers had changed to increased incidence in small- and medium-sized towns, the case of Marília. OBJECTIVES: The aim of this article was to distinguish dengue serotypes circulating during the 2007 Marília outbreak and define their association to demographic and hematological patient profiles, as well as the phylogenetic relationships among the different viruses...
March 6, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28281864/vaccines-licensed-and-in-clinical-trials-for-the-prevention-of-dengue
#6
J Torresi, G Ebert, M Pellegrini
Dengue has become a major global public health threat with almost half of the world's population living in at-risk areas. Vaccination would likely represent an effective strategy for the management of dengue disease in endemic regions, however to date there is only one licensed preventative vaccine for dengue infection. The development of a vaccine against dengue virus (DENV) has been hampered by an incomplete understanding of protective immune responses against DENV. The most clinically advanced dengue vaccine is the chimeric yellow fever-dengue vaccine (CYD) that employs the yellow fever virus 17D strain as the replication backbone (Chimerivax-DEN; CYD-TDV)...
February 14, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28278157/societal-impact-of-dengue-outbreaks-stakeholder-perceptions-and-related-implications-a-qualitative-study-in-brazil-2015
#7
Joël Ladner, Mariana Rodrigues, Ben Davis, Marie-Hélène Besson, Etienne Audureau, Joseph Saba
BACKGROUND: The growing burden of dengue in many countries worldwide and the difficulty of preventing outbreaks have increased the urgency to identify alternative public health management strategies and effective approaches to control and prevent dengue outbreaks. The objectives of this study were to understand the impact of dengue outbreak on different stakeholders in Brazil, to explore their perceptions of approaches used by governmental authorities to control and prevent dengue outbreaks and to define the challenges and implications of preventing future outbreaks...
March 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28255149/unusual-presentation-of-severe-dengue-dengue-maculopathy
#8
K H Chuah, C W K Ng, K Zabri, C L Wong
Dengue infection is epidemic but ocular complications of dengue are less reported. We report a case of dengueinfected patient with disturbing ocular symptoms. He was admitted for severe dengue. Subsequently, he presented with symptoms of bilateral central scotoma with metamorphosia. Fundus examination revealed cotton wool spots and flame-shaped haemorrhages at the macula, with dull foveal light reflex. He was diagnosed with bilateral dengue maculopathy. The patient was managed conservatively with surveillance...
February 2017: Medical Journal of Malaysia
https://www.readbyqxmd.com/read/28255145/infection-associated-haemophagocytic-syndrome-in-severe-dengue-infection-a-case-series-in-a-district-hospital
#9
Y S Y Jasmine, S L Lee, F K Kan
Haemophagocytic lymphohistiocytosis (HLH) is a potentially fatal disorder resulting from uncontrolled hyperinflammatory response. There had been increase in cases of one of the secondary form of HLH, i.e., infectionassociated haemophagocytic syndrome (IAHS) in severe dengue in recent years. However, the condition remains under diagnosed due to lack of awareness compounded by the lack of validated diagnostic criteria. Severe hepatitis with prolonged cytopenias, severe hyperferritinemia, hypofibrinogenemia and persistent fever were evident in all four cases reported...
February 2017: Medical Journal of Malaysia
https://www.readbyqxmd.com/read/28255142/upper-gastrointestinal-haemorrhage-in-severe-dengue-to-scope-or-not-to-scope
#10
C H Lim, N H S Benjamin, F K Kan
Upper gastrointestinal haemorrhage (UGIH) in severe dengue represents a clinical dilemma in term of management. The recommended treatment in dengue with UGIH involves blood product transfusion support and proton pump inhibitor (PPI) infusion. Despite being the mainstay of treatment in non-dengue UGIH, the role of endoscopic haemostatic intervention in severe dengue remains controversial. In the present report, we present a case of severe dengue complicated with upper gastrointestinal haemorrhage successfully underwent early therapeutic endoscopic intervention in a district hospital...
February 2017: Medical Journal of Malaysia
https://www.readbyqxmd.com/read/28217383/management-dilemmas-in-a-rare-case-of-pituitary-apoplexy-in-the-setting-of-dengue-hemorrhagic-fever
#11
S J Balaparameswara Rao, Amey R Savardekar, B N Nandeesh, A Arivazhagan
BACKGROUND: Pituitary apoplexy occurs due to infarction or hemorrhage, within a pituitary adenoma or a nontumorous pituitary gland and can have catastrophic consequences. Dengue hemorrhagic fever (DHF) is a severe manifestation of the spectrum of dengue virus infection and is characterized by high-grade fever, thrombocytopenia, hemorrhagic tendencies, and increased vascular permeability. Cases of incidentalomas complicated by DHF and presenting with apoplexy are extremely rare. CASE DESCRIPTION: We describe the case of a 45-year-old gentleman who suffered an attack of pituitary apoplexy while being treated for DHF...
2017: Surgical Neurology International
https://www.readbyqxmd.com/read/28199323/a-large-scale-biorational-approach-using-bacillus-thuringiensis-israeliensis-strain-am65-52-for-managing-aedes-aegypti-populations-to-prevent-dengue-chikungunya-and-zika-transmission
#12
Catherine A Pruszynski, Lawrence J Hribar, Robert Mickle, Andrea L Leal
BACKGROUND: Aedes aegypti is a container-inhabiting mosquito and a vector of dengue, chikungunya, and Zika viruses. In 2009 several cases of autochthonous dengue transmission were reported in Key West, Florida, USA prompting a comprehensive response to control A. aegypti. In Key West, larvae of this mosquito develop in containers around human habitations which can be numerous and labor intensive to find and treat. Aerial applications of larvicide covering large areas in a short time can be an efficient and economical method to control A...
2017: PloS One
https://www.readbyqxmd.com/read/28192520/holding-back-the-tiger-successful-control-program-protects-australia-from-aedes-albopictus-expansion
#13
Mutizwa Odwell Muzari, Gregor Devine, Joseph Davis, Bruce Crunkhorn, Andrew van den Hurk, Peter Whelan, Richard Russell, James Walker, Peter Horne, Gerhard Ehlers, Scott Ritchie
BACKGROUND: The Asian tiger mosquito, Aedes albopictus, is an important vector of dengue, chikungunya and Zika viruses and is a highly invasive and aggressive biter. Established populations of this species were first recognised in Australia in 2005 when they were discovered on islands in the Torres Strait, between mainland Australia and Papua New Guinea. A control program was implemented with the original goal of eliminating Ae. albopictus from the Torres Strait. We describe the evolution of management strategies that provide a template for Ae...
February 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28187780/levels-of-insecticide-resistance-to-deltamethrin-malathion-and-temephos-and-associated-mechanisms-in-aedes-aegypti-mosquitoes-from-the-guadeloupe-and-saint-martin-islands-french-west-indies
#14
Daniella Goindin, Christelle Delannay, Andric Gelasse, Cédric Ramdini, Thierry Gaude, Frédéric Faucon, Jean-Philippe David, Joël Gustave, Anubis Vega-Rua, Florence Fouque
BACKGROUND: In the Guadeloupe and Saint Martin islands, Aedes aegypti mosquitoes are the only recognized vectors of dengue, chikungunya, and Zika viruses. For around 40 years, malathion was used as a mosquito adulticide and temephos as a larvicide. Since the European Union banned the use of these two insecticide molecules in the first decade of the 21st century, deltamethrin and Bacillus thuringiensis var. israelensis are the remaining adulticide and larvicide, respectively, used in Guadeloupe...
February 10, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28187147/widespread-fear-of-dengue-transmission-but-poor-practices-of-dengue-prevention-a-study-in-the-slums-of-delhi-india
#15
Éric Daudé, Sumit Mazumdar, Vandana Solanki
BACKGROUND: This study has been conducted to throw light on the knowledge and practices related to dengue fever among the poor population living in Delhi's slums. MATERIALS: A household survey was conducted in 2013 among 3,350 households. The households were stratified by a number of variables related to socio-economic status and health events such as hospitalisation. The data collection was completed through face-to-face interviews conducted with the help of 25 field investigators...
2017: PloS One
https://www.readbyqxmd.com/read/28148289/a-rare-case-of-dengue-encephalopathy-complicating-a-term-pregnancy
#16
Lavanya Rajagopala, Ravindra L Satharasinghe, Madhava Karunarathna
BACKGROUND: Dengue fever has an expanded clinical spectrum ranging from an asymptomatic infection to life threatening dengue hemorrhagic fever and refractory shock. Dengue infection in pregnancy can be a diagnostic dilemma, particularly considering the physiological changes in pregnancy and the obstetric complications encountered in clinical practice. Hence the knowledge of its diagnosis and management in its atypical presentations is of paramount importance. Here we report an unusual case of uncomplicated dengue encephalopathy in a term mother, probably the first to be reported from the Indian subcontinent...
February 2, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28138048/clinician-survey-to-determine-knowledge-of-dengue-and-clinical-management-practices-texas-2014
#17
Jessica K Adam, Roman Abeyta, Brian Smith, Linda Gaul, Dana L Thomas, George Han, Tyler M Sharp, Stephen H Waterman, Kay M Tomashek
Dengue, a mosquito-borne viral disease, is increasingly being identified as a cause of outbreaks in the United States. During July-December 2013, a total of three south Texas counties reported 53 laboratory-confirmed dengue cases; 26 were locally acquired, constituting the largest outbreak in Texas since 2005. Because dengue outbreaks are expected to continue in south Texas and early case identification and timely treatment can reduce mortality, we sought to determine clinicians' knowledge of dengue and its clinical management...
January 30, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28114328/pyrethroid-resistance-in-malaysian-populations-of-dengue-vector-aedes-aegypti-is-mediated-by-cyp9-family-of-cytochrome-p450-genes
#18
Intan H Ishak, Basile Kamgang, Sulaiman S Ibrahim, Jacob M Riveron, Helen Irving, Charles S Wondji
BACKGROUND: Dengue control and prevention rely heavily on insecticide-based interventions. However, insecticide resistance in the dengue vector Aedes aegypti, threatens the continued effectiveness of these tools. The molecular basis of the resistance remains uncharacterised in many endemic countries including Malaysia, preventing the design of evidence-based resistance management. Here, we investigated the underlying molecular basis of multiple insecticide resistance in Ae. aegypti populations across Malaysia detecting the major genes driving the metabolic resistance...
January 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28107859/dengue-fever-may-mislead-the-surgeons-when-it-presents-as-an-acute-abdomen
#19
Bingumal Jayasundara, Lalith Perera, Ajith de Silva
OBJECTIVE: To review the management experience of a consecutive series of patients presenting as acute surgical abdomen whom were ultimately diagnosed to have DF (Dengue fever)/DHF (Dengue hemorrhagic fever). METHODS: Clinical data of all cases of apparent acute abdomen (AA) which were later confirmed as having DF/DHF reviewed by two surgical units from December 2012 to December 2013 were analyzed. Initially confirmed patients with DF/DHF who developed abdominal symptoms were not considered...
January 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28104185/causes-and-outcomes-of-sepsis-in-southeast-asia-a-multinational-multicentre-cross-sectional-study
#20
(no author information available yet)
BACKGROUND: Improved understanding of pathogens that cause sepsis would aid management and antimicrobial selection. In this study, we aimed to identify the causative pathogens of sepsis in southeast Asia. METHODS: In this multinational multicentre cross-sectional study of community-acquired sepsis and severe sepsis, we prospectively recruited children (age ≥30 days and <18 years) and adults (age ≥18 years) at 13 public hospitals in Indonesia (n=3), Thailand (n=4), and Vietnam (n=6)...
February 2017: Lancet Global Health
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