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https://www.readbyqxmd.com/read/28821048/a-review-of-chemical-methods-for-the-selective-sulfation-and-desulfation-of-polysaccharides
#1
REVIEW
Emiliano Bedini, Antonio Laezza, Michelangelo Parrilli, Alfonso Iadonisi
Sulfated polysaccharides are known to possess several biological activities, with their sulfation pattern acting as a code able to transmit functional information. Due to their high biological and biomedical importance, in the last two decades many reports on the chemical modification of their sulfate distribution as well as on the regioselective insertion of sulfate groups on non-sulfated polysaccharides appeared in literature. In this Review we have for the first time collected these reports together, categorizing them into three different classes: i) regioselective sulfation reactions, ii) regioselective desulfation reactions, iii) regioselective insertion of sulfate groups through multi-step strategies, and discussing their scope and limitations...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28820690/scaling-up-of-an-innovative-intervention-to-reduce-risk-of-dengue-chikungunya-and-zika-transmission-in-uruguay-in-the-framework-of-an-intersectoral-approach-with-and-without-community-participation
#2
César Basso, Elsa García da Rosa, Rosario Lairihoy, Ruben M Caffera, Ingrid Roche, Cristina González, Ricardo da Rosa, Alexis Gularte, Eduardo Alfonso-Sierra, Max Petzold, Axel Kroeger, Johannes Sommerfeld
To contribute to the prevention of dengue, chikungunya, and Zika, a process of scaling up an innovative intervention to reduce Aedes aegypti habitats, was carried out in the city of Salto (Uruguay) based on a transdisciplinary analysis of the eco-bio-social determinants. The intervention in one-third of the city included the distributions of plastic bags for all households to collect all discarded water containers that were recollected by the Ministry of Health and the Municipality vector control services. The results were evaluated in 20 randomly assigned clusters of 100 households each, in the intervention and control arm...
August 7, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28820685/immune-reconstitution-inflammatory-syndrome-in-hiv-infected-immigrants
#3
María Pérez-Rueda, Michele Hernández-Cabrera, Adela Francés-Urmeneta, Alfonso Angel-Moreno, Elena Pisos-Álamo, Nieves Jaén-Sánchez, Cristina Carranza-Rodríguez, Jose-Luis Pérez-Arellano
Immune reconstitution inflammatory syndrome (IRIS) includes a group of potentially serious inflammatory processes that may be present in HIV-infected patients after initiating highly active antiretroviral therapy (HAART). Paradoxical IRIS is a worsening of symptoms, after an overwhelming response to a previously diagnosed opportunistic infection (OI); unmasking IRIS reveals a previously occult OI. The main objective of the study was to describe the epidemiological, clinical, and outcome data of HIV-infected immigrants, stratified according to high- or low-income countries of origin, who developed IRIS and to compare them with native-born Spanish patients...
August 14, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28819692/molecular-characterization-of-atypical-antigenic-variants-of-canine-rabies-virus-reveals-its-reintroduction-by-wildlife-vectors-in-southeastern-mexico
#4
Fabiola Garcés-Ayala, Nidia Aréchiga-Ceballos, Joanna M Ortiz-Alcántara, Elizabeth González-Durán, Sandra I Pérez-Agüeros, Alfonso Méndez-Tenorio, Belem Torres-Longoria, Irma López-Martínez, Lucía Hernández-Rivas, José Alberto Díaz-Quiñonez, José Ernesto Ramírez-González
Rabies is an infectious viral disease that is practically always fatal following the onset of clinical signs. In Mexico, the last case of human rabies transmitted by dogs was reported in 2006 and canine rabies has declined significantly due to vaccination campaigns implemented in the country. Here we report on the molecular characterization of six rabies virus strains found in Yucatan and Chiapas, remarkably, four of them showed an atypical reaction pattern when antigenic characterization with a reduced panel of eight monoclonal antibodies was performed...
August 17, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28816102/hydrodenticity-to-enhance-relaxivity-of-gadolinium-dtpa-within-crosslinked-hyaluronic-acid-nanoparticles
#5
Maria Russo, Alfonso Maria Ponsiglione, Ernesto Forte, Paolo Antonio Netti, Enza Torino
AIM: The efficacy of gadolinium (Gd) chelates as contrast agents for magnetic resonance imaging remains limited owing to poor relaxivity and toxic effects. Here, the effect of the hydration of the hydrogel structure on the relaxometric properties of Gd-DTPA is explained for the first time and called Hydrodenticity. RESULTS: The ability to tune the hydrogel structure is proved through a microfluidic flow-focusing approach able to produce crosslinked hyaluronic acid nanoparticles, analyzed regarding the crosslink density and mesh size, and connected to the characteristic correlation times of the Gd-DTPA...
August 17, 2017: Nanomedicine
https://www.readbyqxmd.com/read/28815670/commentary-on-wolfgang-loch-s-paper-drives-and-objects-observations-on-the-origins-of-the-emotional-object-world
#6
Alfonso Pola
No abstract text is available yet for this article.
August 2017: International Journal of Psycho-analysis
https://www.readbyqxmd.com/read/28815494/use-of-the-dice-dual-integrase-cassette-exchange-system
#7
Alfonso P Farruggio, Mital S Bhakta, Michele P Calos
When constructing transgenic cell lines via plasmid DNA integration, precise targeting to a desired genomic location is advantageous. It is also often advantageous to remove the bacterial backbone, since bacterial elements can lead to the epigenetic silencing of neighboring DNA. The least cumbersome method to remove the plasmid backbone is recombinase-mediated cassette exchange (RMCE). RMCE is accomplished by arranging recombinase sites in the genome and in a donor plasmid such that a recombinase can both integrate the donor plasmid and excise its bacterial backbone...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28814824/cataract-surgery-in-patients-with-pseudoex-foliation-syndrome-current-updates
#8
REVIEW
Luigi Fontana, Marco Coassin, Alfonso Iovieno, Antonio Moramarco, Luca Cimino
Pseudoexfoliation is a ubiquitous syndrome of multifactorial origin affecting elderly people by increasing the risk of cataract and secondary glaucoma development. Despite modern techniques and technologies for cataract surgery, pseudoexfoliation syndrome represents a challenge for surgeons because of the increased weakness of the zonular apparatus and limited pupil dilation. Due to the inherent difficulties during surgery, the risk of vitreous loss in these patients is several times higher than in cataract patients without pseudoexfoliation...
2017: Clinical Ophthalmology
https://www.readbyqxmd.com/read/28810850/variations-in-diabetes-remission-rates-after-bariatric-surgery-in-spanish-adults-according-to-the-use-of-different-diagnostic-criteria-for-diabetes
#9
María R Alhambra-Expósito, María J Molina-Puerta, María I Prior-Sánchez, Gregorio Manzano-García, Alfonso Calañas-Continente, María A Gálvez-Moreno
BACKGROUND: There are multiple criteria to define remission of type 2 diabetes (DM2) after bariatric surgery but there is not a specific one widely accepted. Our objectives were to compare diagnostic criteria for DM2 remission after bariatric surgery: Criteria from Spanish scientific associations (SEEN/SEEDO/SED) and from the American Diabetes Association (ADA). We also aim to analyse the degree of correlation between these sets of criteria. METHODS: Retrospective observational study in 127 patients undergoing bariatric surgery in a single centre (Hospital Universitario Reina Sofía, Córdoba, Spain) between January 2001 and December 2009...
August 15, 2017: BMC Endocrine Disorders
https://www.readbyqxmd.com/read/28809529/multiphoton-fabrication-of-fibronectin-functionalized-protein-micropatterns-stiffness-induced-maturation-of-cell-matrix-adhesions-in-human-mesenchymal-stem-cells
#10
Jiaoni Ma, Chuen Wai Li, Nan Huang, Xinna Wang, Minghui Tong, Alfonso H W Ngan, Barbara P Chan
Cell-matrix adhesions are important structures governing the interactions between cells and their microenvironment at the cell-matrix interface. Focal complex (FC) and focal adhesion (FA) have been substantially investigated in conventional 2D culture system using fibroblast as in vitro model. However, the formation of more mature types of cell-matrix adhesion in human mesenchymal stem cells (hMSCs), including fabrillar adhesion (FBA) and 3D matrix adhesion (3DMA), have not been fully elucidated. Here, we try to provide some hints on which niche factor(s) dominate(s) the maturation of FBA and 3DMA by using multiphoton fabrication-based micropatterning technique...
August 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28807248/tumor-model-for-surgical-simulation-to-assess-a-minimally-invasive-endoscopic-approach-for-midcheek-mass-removal
#11
Giovanni Dell' Aversana Orabona, Antonio Romano, Paola Bonavolontà, Vincenzo Abbate, Alfonso Manfuso, Giorgio Iaconetta, Bernhard Hirt, Luigi Califano
INTRODUCTION: The midcheek is considered one of the most important facial area due to its involvement in mimic expression and communication. Pathology of this district is complex due to the variety of soft tissue belonging to the face. We propose a surgical simulation, to assess the feasibility of a new minimally invasive endoscopic approach for midcheek mass removal. TECHNICAL REPORT: This study was performed on four cadavers, at the Anatomy Laboratory of the University of Tubingen...
September 2017: Surgical Oncology
https://www.readbyqxmd.com/read/28806636/blood-or-not-blood-that-is-the-question-a-non-destructive-method-for-the-detection-of-blood-contaminated-fingermarks
#12
Alfonso Bentolila, Ira Hartman, Michal Levin-Elad
Working in crime scenes presents a challenge to the forensic scientist, as some surfaces, such as floors and walls, cannot be transferred to the lab for further development and must, therefore, be processed at the crime scene itself. Two main types of latent fingermarks may be encountered in crime scenes: amino acids based and blood contaminated. One of the most common reagents, which are able to develop both types of fingermarks on porous surfaces, is ninhydrin. As blood contaminated fingermarks may be crucial in connecting the suspect to the crime it is important to be able to distinguish between them and natural fingermarks...
August 5, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28806334/cochlear-implantation-in-postlingual-adult-patients-with-long-term-auditory-deprivation
#13
Maria Del Mar Medina, Ruben Polo, Auxiliadora Gutierrez, Alfonso Muriel, Miguel Vaca, Cecilia Perez, Adela Cordero, Ignacio Cobeta
OBJECTIVE: The role of long-term hearing deprivation in cochlear implant performance is not well established, and the limits for implanting an auditory deprived ear are still unknown. The objective of this study was to evaluate the effect of long-term auditory deprivation time on cochlear implant results. STUDY DESIGN: Retrospective case review study. SETTING: Cochlear implantation tertiary referral center. PATIENTS: Adult patients with postlingual deafness...
September 2017: Otology & Neurotology
https://www.readbyqxmd.com/read/28806295/splenules-presenting-as-incidental-gastric-fundic-masses-on-endoscopy
#14
Ammar Khayat, Alfonso M Martinez
No abstract text is available yet for this article.
August 11, 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/28803957/a-standardization-of-the-novelty-suppressed-feeding-test-protocol-in-rats
#15
Arantxa Blasco-Serra, Eva M González-Soler, Ana Cervera-Ferri, Vicent Teruel-Martí, Alfonso A Valverde-Navarro
Tests based on hyponeophagia phenomena are the most widely used to check the efficacy and efficiency of new-generation chronic antidepressant treatments. Even so, these tests lack strict consensus about their methodology, which reduces their validity, reproducibility and makes translatability difficult. Therefore, after an extensive literature review on this subject, we propose a methodological protocol for the Novelty-Suppressed Feeding Test to normalize this situation. Animals were induced to a reserpine-induced depression model and were then chronically treated with duloxetine, desvenlafaxine or vehicle...
August 10, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28803661/daily-bathing-strategies-and-cross-transmission-of-multidrug-resistant-organisms-impact-of-chlorhexidine-impregnated-wipes-in-a-multidrug-resistant-gram-negative-bacteria-endemic-intensive-care-unit
#16
Jesus Ruiz, Paula Ramirez, Esther Villarreal, Monica Gordon, Inmaculada Saez, Alfonso Rodríguez, María Jesús Castañeda, Álvaro Castellanos-Ortega
BACKGROUND: Health-care associated infections are a major cause of morbidity and mortality in critical care units. The aim of this study is to evaluate the effectiveness of chlorhexidine gluconate (CHG)-impregnated wipes in the daily bathing of patients in an intensive care unit (ICU) to prevent cross-transmission and colonization by multidrug-resistant organisms (MDROs) METHODS: Prospective cohort study with an intervention of 11 months. The intervention consisted of using CHG-impregnated wipes for the daily bathing of patients on mechanical ventilation or colonized by MDROs...
August 10, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28802792/fat-mass-fat-free-mass-and-resting-metabolic-rate-in-weight-stable-sleeve-gastrectomy-patients-compared-with-weight-stable-nonoperated-patients
#17
Luigi Schiavo, Giuseppe Scalera, Vincenzo Pilone, Gabriele De Sena, Antonio Iannelli, Alfonso Barbarisi
BACKGROUND: There is evidence that body composition and resting metabolic rate (RMR) in weight-stable patients after Roux-en-Y gastric bypass and duodenal switch is similar to that of nonoperated individuals within the same body mass index (BMI) interval. Currently, data concerning fat mass (FM), fat-free mass (FFM), and RMR on weight-stable patients after sleeve gastrectomy (SG) are lacking. OBJECTIVES: To assess FM, FFM, and RMR, in a selected and homogenous population of weight-stable SG patients (WSSG) and compare them with those obtained from healthy normal weight-stable nonoperated (WSNO) volunteers controls of similar sex, age, and BMI...
July 1, 2017: Surgery for Obesity and related Diseases: Official Journal of the American Society for Bariatric Surgery
https://www.readbyqxmd.com/read/28802780/herringbone-like-hydrodynamic-structures-in-microchannels-a-cfd-model-to-evaluate-the-enhancement-of-surface-binding
#18
Elena Bianchi, Monica Piergiovanni, Chiara Arrigoni, Junji Fukuda, Alfonso Gautieri, Matteo Moretti, Gabriele Dubini
Selected adsorption efficiency of a molecule in solution in a microchannel is strongly influenced by the convective/diffusive mass transport phenomena that supply the target molecule to the adsorption surface. In a standard microchannel with a rectangular cross section, laminar flow regime limits the fluid mixing, thus suggesting that mass transport conditions can be improved by the introduction of herringbone-like structures. Tuning of these geometrical patterns increases the concentration gradient of the target molecule at the adsorption surface...
August 9, 2017: Medical Engineering & Physics
https://www.readbyqxmd.com/read/28801341/tetrabenazine-and-subthalamic-stimulation-in-graft-induced-dyskinesias
#19
Ariel Levy, Alfonso Fasano
No abstract text is available yet for this article.
August 11, 2017: Neurology
https://www.readbyqxmd.com/read/28801180/obesity-as-predictor-of-postoperative-outcomes-in-liver-transplant-candidates-review-of-the-literature-and-future-perspectives
#20
REVIEW
Michele Barone, Maria Teresa Viggiani, Alfonso W Avolio, Andrea Iannone, Maria Rendina, Alfredo Di Leo
BACKGROUND: Current American and European guidelines consider a pre-transplant BMI ≥40kg/m(2) as a relative contraindication for liver transplantation but this recommendation is graded as uncertain and requires further research. Moreover, conflicting results are reported on the predictive value of BMI 30-39.9kg/m(2) on post-transplant complication and mortality risk. AIM: This study analyzed the data of the literature on the effect of all three BMI classes of obesity on postoperative outcomes in liver transplantation...
July 22, 2017: Digestive and Liver Disease
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