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calcium silicate nanoparticles

Leticia Boldrin Mestieri, Ana Lívia Gomes-Cornélio, Elisandra Márcia Rodrigues, Gisele Faria, Juliane Maria Guerreiro-Tanomaru, Mário Tanomaru-Filho
The aim of this study was to evaluate the cytotoxicity and bioactivity of calcium silicate-based cements combined with niobium oxide (Nb2O5) micro and nanoparticles, comparing the response in different cell lines. This evaluation used four cell lines: two primary cultures (human dental pulp cells - hDPCs and human dental follicle cells - hDFCs) and two immortalized cultures (human osteoblast-like cells - Saos-2 and mouse periodontal ligament cells - mPDL). The tested materials were: White Portland Cement (PC), mineral trioxide aggregate (MTA), white Portland cement combined with microparticles (PC/Nb2O5µ) or nanoparticles (PC/Nb2O5n) of niobium oxide (Nb2O5)...
January 2017: Brazilian Dental Journal
Ahmed M Youssef, Amany M El-Nahrawy, Ali B Abou Hammad
Hybrid Chitosan/Poly ethylene glycol/calcium silicate (CS/PEG/calcium silicate) nanocomposite modified with different two types, zinc oxide nanoparticles (ZnO-NPs) and tartrazine dye (E102) were prepared by sol gel method and the characterization of their structure and biological properties were carried out in order to evaluate the possible use in optical and biomedical fields. The hybrid CS/PEG/calcium silicate complex formations have been established by X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopic analysis...
April 2017: International Journal of Biological Macromolecules
Fernando Vazquez-Garcia, Mário Tanomaru-Filho, Gisselle Moraima Chávez-Andrade, Roberta Bosso-Martelo, Maria Inês Basso-Bernardi, Juliane Maria Guerreiro-Tanomaru
Mineral trioxide aggregate (MTA) and Portland cement (PC) are calcium silicate cements. They have similar physicochemical, mechanical and biological properties. The addition of zirconium oxide (ZrO2) to PC provides radiopacity. Silver nanoparticles (AgNPs) may improve some properties of cements. The aim of this study was to evaluate the effect of AgNPs on physicochemical/mechanical properties and antibacterial activity of white MTA (WMTA) and PC associated with ZrO2. The following materials were evaluated: WMTA; PC 70% + ZrO2 30%; WMTA+ AgNPs; and PC 70% + ZrO2 30% + AgNPs...
September 2016: Brazilian Dental Journal
Ching-Yuang Huang, Tsui-Hsien Huang, Chia-Tze Kao, Yuan-Haw Wu, Wan-Chen Chen, Ming-You Shie
INTRODUCTION: Calcium silicate (CS) -based materials play an important role in the development of endodontic materials that induce bone/cementum tissue regeneration and inhibit bacterial viability. The aim of this study was to prepare novel mesoporous CS (MesoCS) nanoparticles that have osteogenic, drug delivery, and antibacterial characteristics for endodontic materials and also have an excellent ability to develop apatite mineralization. METHODS: The MesoCS nanoparticles were prepared using sol-gel methods...
January 2017: Journal of Endodontics
D N Leonard, R Hellmann
Most liquid cell transmission electron microscopy (LC TEM) studies focus on nanoparticles or nanowires, in large part because the preparation and study of materials in this size range is straightforward. By contrast, this is not true for samples in the micrometre size range, in large part because of the difficulties associated with sample preparation starting from a 'bulk' material. There are also many advantages inherent to the study of micrometre-sized samples compared to their nanometre-sized counterparts...
December 5, 2016: Journal of Microscopy
Wei Fan, Yanyun Li, Qing Sun, Tengjiao Ma, Bing Fan
BACKGROUND: In infected periapical tissues, Enterococcus faecalis is one of the most common dominant bacteria. Chlorhexidine has been proved to show strong antibacterial ability against E. faecalis but is ineffective in promoting mineralization for tissues around root apex. Mesoporous calcium-silicate nanoparticles are newly synthesized biomaterials with excellent ability to promote mineralization and carry-release bioactive molecules in a controlled manner. In this study, mesoporous calcium-silicate nanoparticles were functionalized with chlorhexidine and their releasing profile, antibacterial ability, effect on cell proliferation and in vitro mineralization property were evaluated...
October 21, 2016: Journal of Nanobiotechnology
Weijie You, Yali Weng, Xiu Wang, Zanyong Zhuang, Yan Yu
Using the porous framework of CaO as templates and reagents, we explored a surfactant-free and economical method for preparing calcium silicate hydrate (CSH) hierarchically ordered nanostructures. Incorporation of SiO2 nanoparticles into the CaO framework, followed by a reaction assisted by hydrothermal treatment, resulted in the formation of CSH with well-defined morphologies. The structural features of CSH were characterized by 3-D hierarchical networks, wherein nanofibers assembled to form nanosheets, and nanosheets assembled to form hierarchically ordered structures...
December 14, 2016: ACS Applied Materials & Interfaces
Sudhanshu Shekhar, Abhijit Roy, Daeho Hong, Prashant N Kumta
Nanostructured ceramic particles, particularly, nanoparticles of calcium phosphate (CaP) remain an attractive option among the various types of non-viral gene delivery vectors studied because of their safety, biocompatibility, biodegradability, and ease of handling as well as their adsorptive capacity for DNA. We have accordingly developed an enhanced version of nanostructured calcium phosphates (NanoCaPs), by substituting known amounts of silicate for phosphate in the hydroxyapatite (HA) lattice (NanoSiCaPs)...
December 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
Amany M El-Nahrawy, Ahmed I Ali, Ali B Abou Hammad, Ahmed M Youssef
Chitosan (CS)/calcium silicate nanocomposites pure and doped with Ag ions (1, 2mol%) were prepared via sol-gel method. The prepared CS/calcium silicate nanocomposites were investigated through X-ray diffraction (XRD), Fourier transforms infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). The XRD results, indicating that after increasing the Ag ions in the CS/calcium silicate nanocomposite the crystallinity degree increased regularly in the prepared nanocomposites accompanying to the continuously rearrangement in the internal structure of nanocomposite under the effect of inorganic nanoparticles...
December 2016: International Journal of Biological Macromolecules
Juliane Maria Guerreiro-Tanomaru, Fernando Antonio Vázquez-García, Roberta Bosso-Martelo, Maria Inês Basso Bernardi, Gisele Faria, Mario Tanomaru
OBJECTIVE: Mineral Trioxide Aggregate (MTA) is a calcium silicate cement composed of Portland cement (PC) and bismuth oxide. Hydroxyapatite has been incorporated to enhance mechanical and biological properties of dental materials. This study evaluated physicochemical and mechanical properties and antibiofilm activity of MTA and PC associated with zirconium oxide (ZrO2) and hydroxyapatite nanoparticles (HAn). MATERIAL AND METHODS: White MTA (Angelus, Brazil); PC (70%)+ZrO2 (30%); PC (60%)+ZrO2 (30%)+HAn (10%); PC (50%)+ZrO2 (30%)+HAn (20%) were evaluated...
May 2016: Journal of Applied Oral Science: Revista FOB
Jinyoung Kang, Jinmyoung Joo, Ester J Kwon, Matthew Skalak, Sazid Hussain, Zhi-Gang She, Erkki Ruoslahti, Sangeeta N Bhatia, Michael J Sailor
Calcium ions react with silicic acid released from dissolving porous silicon nanoparticles to create an insoluble calcium silicate shell. The calcium silicate shell traps and protects an siRNA payload, which can be delivered to neuronal tissues in vitro or in vivo. Gene delivery is enhanced by the action of targeting and cell-penetrating peptides attached to the calcium silicate shell.
September 2016: Advanced Materials
Patrick A Bonnaud, Christophe Labbez, Ryuji Miura, Ai Suzuki, Naoto Miyamoto, Nozomu Hatakeyama, Akira Miyamoto, Krystyn J Van Vliet
Calcium-silicate-hydrate (or C-S-H), an inosilicate, is the major binding phase in cement pastes and concretes and a porous hydrated material made up of a percolated and dense network of crystalline nanoparticles of a mean apparent spherical diameter of ∼5 nm that are each stacks of multiple C-S-H layers. Interaction forces between these nanoparticles are at the origin of C-S-H chemical, physical, and mechanical properties at the meso- and macroscales. These particle interactions and the resulting properties may be affected significantly by nanoparticle density and environmental conditions such as the temperature, relative humidity, or concentration of chemical species in the bulk solution...
February 21, 2016: Nanoscale
Katerina Ioannidou, Konrad J Krakowiak, Mathieu Bauchy, Christian G Hoover, Enrico Masoero, Sidney Yip, Franz-Josef Ulm, Pierre Levitz, Roland J-M Pellenq, Emanuela Del Gado
Strength and other mechanical properties of cement and concrete rely upon the formation of calcium-silicate-hydrates (C-S-H) during cement hydration. Controlling structure and properties of the C-S-H phase is a challenge, due to the complexity of this hydration product and of the mechanisms that drive its precipitation from the ionic solution upon dissolution of cement grains in water. Departing from traditional models mostly focused on length scales above the micrometer, recent research addressed the molecular structure of C-S-H...
February 23, 2016: Proceedings of the National Academy of Sciences of the United States of America
G Faccio, M B Bannwarth, C Schulenburg, V Steffen, D Jankowska, M Pohl, R M Rossi, K Maniura-Weber, L F Boesel, M Richter
Silicate nanoparticles with immobilized FRET-based biosensors were developed for the detection of glucose and maltose. Immobilization of the protein biosensor in the nanoparticle was achieved through specific interaction between the hexa-histidine tag of the protein and a calcium-silicate complex of the silica matrix. Encapsulation of the biosensors preserved the affinity for the respective sugar. Compared to the free biosensors, encapsulation had a stabilizing effect on the biosensor towards chemical and thermal denaturation...
June 20, 2016: Analyst
Alireza Valanezhad, Tetsuro Odatsu, Koichi Udoh, Takanobu Shiraishi, Takashi Sawase, Ikuya Watanabe
In the present study, sol-gel derived nanoparticle calcium silicate bioactive glass was added to the resin-modified light cure glass-ionomer cement to assess the influence of additional bioactive glass nanoparticles on the mechanical and biological properties of resin-modified glass-ionomer cement. The fabricated bioactive glass nanoparticles added resin-modified glass-ionomer cements (GICs) were immersed in the phosphate buffer solution for 28 days to mimic real condition for the mechanical properties. Resin-modified GICs containing 3, 5 and 10 % bioactive glass nanoparticles improved the flexural strength compared to the resin-modified glass-ionomer cement and the samples containing 15 and 20 % bioactive glass nanoparticles before and after immersing in the phosphate buffer solution...
January 2016: Journal of Materials Science. Materials in Medicine
Mohammad Ali Saghiri, Armen Asatourian, Jafar Orangi, Christine M Sorenson, Nader Sheibani
Trace elements play critical roles in angiogenesis events. The effects of nitrogen, iron, selenium, phosphorus, gold, and calcium were discussed in part I. In part II, we evaluated the effect of chromium, silicon, zinc, copper, and sulfur on different aspects of angiogenesis, with critical roles in healing and regeneration processes, and undeniable roles in tumor growth and cancer therapy. This review is the second of series that serves as an overview of the role of inorganic elements in regulation of angiogenesis and vascular function...
October 2015: Critical Reviews in Oncology/hematology
Aldo Del Carpio-Perochena, Anil Kishen, Annie Shrestha, Clovis Monteiro Bramante
INTRODUCTION: The aim of this study was to evaluate the efficacy of carboxymethyl chitosan (CMCS) and chitosan nanoparticles (CNps) to inactivate bacteria and prevent biofilm formation at sealer-dentin interfaces. METHODS: The study was divided into 3 stages: first stage, the experiment was conducted to analyze the antibacterial properties of CMCS in different formulations against biofilms; second stage, direct-contact and membrane-restricted methods were used to evaluate the antibacterial properties of an epoxy resin (ThermaSeal Plus; Dentsply Tulsa Dental, Tulsa, OK) and calcium silicate (MTA Fillapex; Angelus SA, Londrina, PR, Brazil) based-sealers with or without CNps; and third stage, biofilm formation at the sealer dentin interfaces of root dentin treated with CMCS and filled with gutta-percha and CNp incorporated sealer were analyzed after 1- and 4-week aging periods...
August 2015: Journal of Endodontics
Roberta Bosso-Martelo, Juliane M Guerreiro-Tanomaru, Raqueli Viapiana, Fabio Luiz C Berbert, Marco Antonio Hungaro Duarte, Mário Tanomaru-Filho
OBJECTIVES: The objective of this paper was to evaluate the physicochemical properties of calcium silicate cements with different chemical compositions, associated with radiopacifying agents. MATERIALS AND METHODS: Mineral trioxide aggregate (MTA) Angelus, calcium silicate cement with additives (CSC), and resinous calcium silicate cement (CSCR) were evaluated, with the addition of the following radiopacifiers: microparticles (micro) or nanoparticles (nano) of zirconium oxide (ZrO(2)), niobium oxide (Nb(2)O(5)), bismuth oxide (Bi(2)O(3)), or calcium tungstate (CaWO(4))...
January 2016: Clinical Oral Investigations
Rekha R Sehgal, S I Roohani-Esfahani, Hala Zreiqat, Rinti Banerjee
Controlled delivery of biological cues through synthetic scaffolds to enhance the healing capacity of bone defects is yet to be realized clinically. The purpose of this study was development of a bioactive tissue-engineered scaffold providing the sustained delivery of an osteoinductive drug, dexamethasone disodium phosphate (DXP), encapsulated within chitosan nanoparticles (CN). Porous baghdadite (BD; Ca3 ZrSi2 O9 ) scaffolds, a zirconia-modified calcium silicate ceramic, was coated with DXP-encapsulated CN nanoparticles (DXP-CN) using nanostructured gellan and xanthan hydrogel (GX)...
April 2017: Journal of Tissue Engineering and Regenerative Medicine
Manuel Monasterio, Juan J Gaitero, Edurne Erkizia, Ana M Guerrero Bustos, Luis A Miccio, Jorge S Dolado, Silvina Cerveny
In this work we study the influence of adding nano-silica (SiO2, Nyasil™) and aminopropyl (-(CH2)3-NH2,) functionalized silica nanoparticles (Stoga) during the synthesis of calcium-silicate-hydrate (C-S-H gel). Characterization by solid state (29)Si NMR and ATR-FTIR spectroscopy showed that the addition of both particle types increases the average length of the silicate chains in C-S-H gel being this effect slightly more important in the case of Stoga particles. In addition, (13)C NMR and XPS confirmed that the aminopropyl chain remains in the final product cleaved to silicon atoms at the end of the silicate chain of C-S-H gel whereas XRD measurements showed that this result in an increment in the basal distance compared with ordinary CSH...
July 15, 2015: Journal of Colloid and Interface Science
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