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Gargi Mahapatra, Ashwathy Varughese, Qinqin Ji, Icksoo Lee, Jenney Liu, Asmita Vaishnav, Christopher Sinkler, Alexandr A Kapralov, Carlos T Moraes, Thomas H Sanderson, Timothy L Stemmler, Lawrence I Grossman, Valerian E Kagan, Joseph S Brunzelle, Arthur R Salomon, Brian F P Edwards, Maik Hüttemann
Mammalian cytochrome c (Cytc) plays a key role in cellular life and death decisions, functioning as an electron carrier in the electron transport chain (ETC) and as a trigger of apoptosis when released from the mitochondria. However, its regulation is not well understood. We show that the major fraction of Cytc isolated from kidneys is phosphorylated on Thr28, leading to a partial inhibition of respiration in the reaction with cytochrome c oxidase. To further study the effect of Cytc phosphorylation in vitro, we generated Thr28Glu phosphomimetic Cytc, revealing superior behavior regarding protein stability and its ability to degrade reactive oxygen species compared to wild-type unphosphorylated Cytc...
October 7, 2016: Journal of Biological Chemistry
Asmita Gupta, Manju Bansal
Mechanical unfolding studies on Ribonucleic Acid (RNA) structures are a subject of tremendous interest as they shed light on the principles of higher order assembly of these structures. Pseudoknotting is one of the most elementary ways in which this higher order assembly is achieved as discrete secondary structural units in RNA are brought in close proximity to form a tertiary structure. Using steered molecular dynamics (SMD) simulations, we have studied the unfolding of five RNA pseudoknot structures that differ from each other either by base substitutions in helices or loops...
October 10, 2016: Physical Chemistry Chemical Physics: PCCP
Niharika Singh, Amrita Patil, Asmita Prabhune, Mamta Raghav, Gunjan Goel
The present study investigates the role of quorum sensing (QS) molecules expressed by C. sakazakii in biofilm formation and extracellular polysaccharide expression. The QS signaling was detected using Chromobacterium violaceum 026 and Agrobacterium tumefaciens NTL4(pZLR4) based bioassay. Long chain N-acyl-homoserine lactones (AHLs) with C6- C18 chain length were identified using High Performance Liquid Chromatography and Liquid Chromatography-High Resolution Mass Spectrometry. A higher Specific Biofilm Formation (SBF) index (p < 0...
October 7, 2016: Virulence
Robin Medhi, Asmita Mishra, Jatinkumar Vishnubhai Patel
No abstract text is available yet for this article.
September 27, 2016: International Urogynecology Journal
Farhad Khimani, Daniel K Jeong, Branko Miladinovic, Taiga Nishihori, Ernesto Ayala, Frederick Locke, Asmita Mishra, Julio Chavez, Bijal Shah, Kenneth Gage, Mohamed A Kharfan-Dabaja
It is unclear if persistent splenomegaly in the presence of a negative positron emission tomography (PET) scan before allogeneic hematopoietic cell transplantation (HCT) influences post-transplantation outcomes in patients with lymphoma. We retrospectively reviewed records of 152 patients who underwent allogeneic HCT for various lymphomas. Centralized review of pretransplantation computed tomography (CT) and PET images was performed. Spleen volume (SV) was measured using the freehand volume segmentation tool in AW Workstation software (General Electric, Waukesha, WI)...
September 19, 2016: Biology of Blood and Marrow Transplantation
Narendranath Epperla, Namrata Shah, Mehdi Hamadani, Kristin Richardson, Jonathan T Kapke, Asmita Patel, Sravanthi P Teegavarapu, George Carrum, Parameswaran N Hari, Sai R Pingali, Reem Karmali, Timothy S Fenske
BACKGROUND: For patients with relapsed or refractory diffuse large B-cell lymphoma (DLBCL), autologous hematopoietic cell transplantation (auto-HCT) is commonly used. After auto-HCT, DLBCL patients are often monitored with surveillance imaging. However, there is little evidence to support this practice. PATIENTS AND METHODS: We performed a multicenter retrospective study of DLBCL patients who underwent auto-HCT (n = 160), who experienced complete remission after transplantation, and who then underwent surveillance imaging...
August 29, 2016: Clinical Lymphoma, Myeloma & Leukemia
Parul Dubey, Sugam Kumar, Vinod K Aswal, Sapna Ravindranathan, Pattuparambil R Rajamohanan, Asmita Prabhune, Anuya Nisal
Silk fibroin (SF) protein, produced by silkworm Bombyx mori, is a promising biomaterial, while sophorolipid (SL) is an amphiphilic functional biosurfactant synthesized by nonpathogenic yeast Candida bombicola. SL is a mixture of two forms, acidic (ASL) and lactonic (LSL), which when added to SF results in accelerated gelation of silk fibroin. LSL is known to have multiple biological functionalities and hence hydrogels of these green molecules have promising applications in the biomedical sector. In this work, SANS, NMR, and rheology are employed to examine the assembling properties of individual and mixed SLs and their interactions with SF to understand the mechanism that leads to rapid gelation...
October 10, 2016: Biomacromolecules
Asmita Parihar, Sonal Sharma
No abstract text is available yet for this article.
July 2016: Journal of Clinical and Diagnostic Research: JCDR
Rajesh Venkitakrishnan, Kallikunnel Sayed Mohamed Aziz, Asmita Mehta
No abstract text is available yet for this article.
July 2016: Lung India: Official Organ of Indian Chest Society
Chabita Saha, Rahul Kumar, Asmita Das
Histones are associated with DNA to form nucleosome essential for chromatin structure and major nuclear processes like gene regulation and expression. Histones consist of H1, H2A, H2B and H3, H4 type proteins. In the present study combined histones from calf thymus were complexed with ct DNA and their binding affinities were measured fluorimetrically. All the five histones were resolved on SDS page and their binding with DNA was visualised. The values of biding affinities varied with pH and salt concentration...
August 17, 2016: Journal of Biomolecular Structure & Dynamics
Pallab Datta, Asmita Ray
Phosphate groups chemically grafted onto polymer substrates can be used as biomimetic analogs for in vitro studying of function of biomacromolecules and also as tissue substitutes in clinical conditions of organ loss. Despite this inspiration, studies correlating effect of degree of phosphate grafting of a polymer on fabrication and biological properties of polymers are lacking. In this work, N-methylene phosphonic chitosan (PC) with different degrees of phosphate contents were synthesized and the effect of phosphate grafting on electrospinning behavior of substituted polymers is investigated...
2016: Progress in biomaterials
Asmita Mishra, Claudio Anasetti
Allogeneic hematopoietic stem cell transplantation (HSCT) is a potentially curative option for patients with myelodysplastic syndrome (MDS). Because MDS predominantly affects an older population, age-associated comorbidities can preclude patients from cure. HSCT is associated with the risk of morbidity and mortality; however, with safer conditioning regimens and improved supportive care, eligible patients with an appropriately matched donor can receive this therapy without exclusion by older age alone. We discuss the role of improved MDS prognostic scoring systems and molecular testing for selection for HSCT, and review the pre-HSCT tolerability assessment required for this advanced aged population...
August 2016: Clinical Lymphoma, Myeloma & Leukemia
Asmita De, Hirak Jyoti Raj, Prasanta Kumar Maiti
Morganella morganii is a member of Enterobacteriaceae family, whose natural habitat is the human gastrointestinal tract. It rarely causes infection alone and is generally encountered in immunosuppressed patients. Osteoarticular pathologies are not commonly observed with Morganella morganii and infections by it have high mortality rate. Biofilm colonization is a causative factor behind the chronicity and/or refractoriness of certain infections. Biofilms colonize on inert medical devices, prosthesis, fibrosed tissues, sinus tracts as well as dead bones as in case of chronic osteomyelitis...
June 2016: Journal of Clinical and Diagnostic Research: JCDR
Niharika Singh, Amrita Patil, Asmita Prabhune, Gunjan Goel
The present study investigated plant extracts for their anti-quorum-sensing (QS) potential to inhibit the biofilm formation in Cronobacter sakazakii strains. The bioassay based on loss of pigment production by Chromobacterium violaceum 026 and Agrobacterium tumefaciens NTL4(pZLR4) was used for initial screening of the extracts. Further, the effect of extracts on the inhibition of QS-mediated biofilm in C. sakazakii isolates was evaluated using standard crystal violet assay. The effect on biofilm texture was studied using SYTO9 staining and light and scanning electron microscopy...
September 2016: Microbiology
Jayeeta Das, Asmita Samadder, Jesmin Mondal, Suresh K Abraham, Anisur Rahman Khuda-Bukhsh
Chlorophyllin (CHL), a sodium-copper-salt derived from chlorophyll, has been widely used as a food-dye, also reportedly having some anti-cancer effect. We tested if PLGA-loaded CHL (NCHL) could have additional protective abilities through its faster and targeted drug delivery in cancer cells. Physico-chemical characterization of NCHL was done through atomic-force microscopy and UV-spectroscopy. NCHL demonstrated greater ability of drug uptake and strong anti-cancer potentials in non-small cell lung cancer cells, A549, as revealed from data of% cell viability, generation of reactive-oxygen-species and expression of bax, bcl2, caspase3, p53 and cytochrome c proteins...
September 2016: Environmental Toxicology and Pharmacology
Huanhuan Joyce Chen, Zhubo Wei, Jian Sun, Asmita Bhattacharya, David J Savage, Rita Serda, Yuri Mackeyev, Steven A Curley, Pengcheng Bu, Lihua Wang, Shuibing Chen, Leona Cohen-Gould, Emina Huang, Xiling Shen, Steven M Lipkin, Neal G Copeland, Nancy A Jenkins, Michael L Shuler
Refined cancer models are needed to bridge the gaps between cell line, animal and clinical research. Here we describe the engineering of an organotypic colon cancer model by recellularization of a native human matrix that contains cell-populated mucosa and an intact muscularis mucosa layer. This ex vivo system recapitulates the pathophysiological progression from APC-mutant neoplasia to submucosal invasive tumor. We used it to perform a Sleeping Beauty transposon mutagenesis screen to identify genes that cooperate with mutant APC in driving invasive neoplasia...
August 2016: Nature Biotechnology
Shuvadeep Maity, Asher Rajkumar, Latika Matai, Ajay Bhat, Asmita Ghosh, Ganesh Agam, Simarjot Kaur, Niraj R Bhatt, Arnab Mukhopadhyay, Shantanu Sengupta, Kausik Chakraborty
Reductive stress leads to the loss of disulfide bond formation and induces the unfolded protein response of the endoplasmic reticulum (UPR(ER)), necessary to regain proteostasis in the compartment. Here we show that peroxide accumulation during reductive stress attenuates UPR(ER) amplitude by altering translation without any discernible effect on transcription. Through a comprehensive genetic screen in Saccharomyces cerevisiae, we identify modulators of reductive stress-induced UPR(ER) and demonstrate that oxidative quality control (OQC) genes modulate this cellular response in the presence of chronic but not acute reductive stress...
July 19, 2016: Cell Reports
Asmita Ashok Patil, Vijaya Laxman Chaudhari
PURPOSE: The educational environment (EE) plays a very important role in effective student learning. The Dundee Ready Education Environment Measure (DREEM) is a validated tool to assess the EE. This study aimed to collect baseline information about our medical student's perception of the EE, and to identify areas of strengths and weaknesses as well as scope for improvements in the current EE. METHODS: Medical students and interns were included in this cross-sectional study...
September 2016: Korean Journal of Medical Education
Asmita A Wele, Nikhil U Pendse, Shrikant P Takle, Raghunath V Burase, Sanjay V Patil, Supriya Bhalerao, Abhay M Harsulkar
INTRODUCTION: Vasarishta built upon Mritasanjeevani Sura (MS) is a polyherbal hydro-alcoholic anti-asthmatic formulation which is administered in a dose of 1 ml instead of standard dose 40 ml, generally advocated for any "Asava-Arishta" in Ayurveda. AIM: The present study was aimed at finding out rationale for the peculiar distillation process to manufacture MS followed by Sthapana process to make Vasarishta. It was further aimed to find out difference in Vasarishta samples manufactured by purely fermentation process and the peculiar method mentioned above...
July 2015: Ayu
Jayeeta Das, Asmita Samadder, Sreemanti Das, Avijit Paul, Anisur Rahman Khuda-Bukhsh
OBJECTIVES: This study examined the relative efficacies of a derivative of betulinic acid (dBA) and its poly (lactide- co-glycolide) (PLGA) nano-encapsulated form in A549 lung cancer cells in vivo and in co-mutagen [sodium arsenite (SA) + benzo]undefined[a]pyrene (BaP)]-induced lung cancer in mice in vivo. METHODS: dBA was loaded with PLGA nanoparticles by using the standard solvent displacement method. The sizes and morphologies of nano-dBA (NdBA) were determined by using transmission electron microscopy (TEM), and their intracellular localization was verified by using confocal microscopy...
March 2016: Journal of Pharmacopuncture
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