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Liangyu Chen, Yixue Xue, Jian Zheng, Xiaobai Liu, Jing Liu, Jiajia Chen, Zhen Li, Zhuo Xi, Hao Teng, Ping Wang, Libo Liu, Yunhui Liu
The blood-tumor barrier (BTB) hinders delivery of chemotherapeutic drugs to tumors in the brain; previous studies have shown that the BTB can be selectively opened by endothelial monocyte activating polypeptide-II (EMAP-II), but the specific mechanism involved remains elusive. In this study, we found that microRNA-429 (miR-429) expression in glioma vascular endothelial cells (GECs) was far lower than in human brain microvascular endothelial cells (ECs). miR-429 had lower expression in GECs and glioma tissues compared to ECs or normal tissues of the brain...
2018: Frontiers in Molecular Neuroscience
Dai Sugimoto, Benton E Heyworth, Jeffery J Brodeur, Dennis E Kramer, Mininder S Kocher, Lyle J Micheli
CONTEXT: Recent epidemiology studies indicated a steady increase of anterior cruciate ligament (ACL) injury in young athletes. ACL reconstruction (ACLR) is currently a standard of the care, but effect of ACLR graft including bone patellar tendon bone (BTB), hamstring tendon (HT), or iliotibial band (ITB) on balance and hop performance is understudied. OBJECTIVE: To compare balance and hop deficits between uninvolved and reconstructed limbs in each autograft type (aim 1) and among the three autograft groups (aim 2)...
February 21, 2018: Journal of Sport Rehabilitation
Shuyue Huang, Mengmeng Zhao, Mahesh B Dawadi, Yuhang Cai, Yakov Lapitsky, David A Modarelli, Nicole S Zacharia
HYPOTHESIS: Complex coacervates are capable of easily partitioning solutes within them based on relative affinities of solute-water and solute-polyelectrolyte pairs, as the coacervate phase has low surface tension with water, facilitating the transport of small molecules into the coacervate phase. The uptake of small molecules is expected to influence the physicochemical properties of the complex coacervate, including the hydrophobicity within coacervate droplets, phase boundaries of coacervation and precipitation, solute uptake capacity, as well as the coacervate rheological properties...
February 9, 2018: Journal of Colloid and Interface Science
Irfan Khattak, Muhammad Hassan Mushtaq, Sultan Ayaz, Sajid Ali, Anwar Sheed, Javed Muhammad, Muhammad Luqman Sohail, Haq Amanullah, Irshad Ahmad, Sadeeq Ur Rahman
Prevalence of zoonotic Mycobacterium bovis (bTB) disease in human population is underreported from the North of Pakistan. Here, we report on the proportion of human bTB disease among the overall TB patients, drug resistance pattern of bTB isolates, and knowledge, attitude, and practices (KAP)-based analysis of bTB disease. For this purpose, sputum samples from a total of 300 clinically diagnosed TB patients and 100 randomly selected school children suspected of pulmonary TB were processed by culture as well as polymerase chain reaction (PCR) for isolation, identification, and confirmation of Mycobacterium tuberculosis (mTB) and bTB species...
February 17, 2018: Advances in Experimental Medicine and Biology
Haiqi Chen, Wing-Yee Lui, Dolores D Mruk, Xiang Xiao, Renshan Ge, Qingquan Lian, Will M Lee, Bruno Silvestrini, C Yan Cheng
The blood-testis barrier is a unique ultrastructure in the mammalian testis, located near the basement membrane of the seminiferous tubule that segregates the seminiferous epithelium into the basal and the adluminal (apical) compartment. Besides restricting paracellular and transcellular passage of biomolecules (e.g., paracrine factors, hormones), water, electrolytes, and other substances including toxicants and/or drugs to enter the adluminal compartment of the epithelium, the BTB is an important ultrastructure that supports spermatogenesis...
2018: Methods in Molecular Biology
Qing Wen, Elizabeth I Tang, Nan Li, Dolores D Mruk, Will M Lee, Bruno Silvestrini, C Yan Cheng
The blood-testis barrier (BTB) is an important ultrastructure in the testis that supports meiosis and postmeiotic spermatid development since a delay in the establishment of a functional Sertoli cell barrier during postnatal development in rats or mice by 17-20 day postpartum (dpp) would lead to a delay of the first wave of meiosis. Furthermore, irreversible disruption of the BTB by toxicants also induces infertility in rodents. Herein, we summarize recent findings that BTB dynamics (i.e., disassembly, reassembly, and stabilization) are supported by the concerted efforts of the actin- and microtubule (MT)-based cytoskeletons...
2018: Methods in Molecular Biology
Barbara Bilinska, Anna Hejmej, Malgorzata Kotula-Balak
One approach to visualize internal structures of the testis is histological sectioning of the material. The use of testicular samples allows a detailed analysis of the structure of both seminiferous tubules and the interstitial space. It is worth noting that key role in the control of germ cell development is assigned to Sertoli cells. Thus, in this chapter the special reference is made on visualization of Sertoli cells in the seminiferous epithelium in which they create a specialized microenvironment to support the germ cell development through the formation of the blood-testis barrier (BTB)...
2018: Methods in Molecular Biology
Qing Wen, Nan Li, Xiang Xiao, Wing-Yee Lui, Darren S Chu, Chris K C Wong, Qingquan Lian, Renshan Ge, Will M Lee, Bruno Silvestrini, C Yan Cheng
Germ cell differentiation during the epithelial cycle of spermatogenesis is accompanied by extensive remodeling at the Sertoli cell-cell and Sertoli cell-spermatid interface to accommodate the transport of preleptotene spermatocytes and developing spermatids across the blood-testis barrier (BTB) and the adluminal compartment of the seminiferous epithelium, respectively. The unique cell junction in the testis is the actin-rich ectoplasmic specialization (ES) designated basal ES at the Sertoli cell-cell interface, and the apical ES at the Sertoli-spermatid interface...
February 12, 2018: Cell Death & Disease
Y Xue, W N Gao, F Chen, B B Ma, F Zhou, Z G Hu, T Long, Z Q Zhao
Cluster differentiation antigen 14 (CD14) is an important pattern recognition receptor protein in innate immunity. The aim of this study was to identify and assess the association of single-nucleotide polymorphisms (SNPs) in the CD14 gene with susceptibility to bovine tuberculosis (BTB) in Chinese Holstein cows. DNA samples from 517 Chinese Holstein cows (257 tuberculosis positive cases and 259 healthy controls) were collected from dairy farms in China. SNPs in the entire CD14 gene, including exonic regions, intronic regions and close to the 5'- and 3'-terminal untranslated regions, were detected by PCR, followed by direct sequencing...
February 2018: Veterinary Journal
Yongxin Yu, Ruguo Shang, Yunzhou Chen, Jiehua Li, Zhichao Liang, Jianwei Hu, Kai Liu, Chao Chen
Increasing studies identify that zinc finger and BTB domain containing 4 (ZBTB4) functions as a tumor suppressor in human cancer. Underexpression of ZBTB4 is correlated with poor survival of breast cancer patients. However, the expression of ZBTB4 and its possible function remain unknown in Ewing sarcoma (ES). To clarify these issues, we investigated the expression difference between ES and normal tissues based on Gene Expression Omnibus (GEO) data from R2: Genomics Analysis and Visualization Platform (http://r2...
February 6, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
G Ciaravino, A García-Saenz, S Cabras, A Allepuz, J Casal, I García-Bocanegra, A De Koeijer, S Gubbins, J L Sáez, D Cano-Terriza, S Napp
In Spain, despite years of efforts to eradicate bovine tuberculosis (bTB), the disease is still endemic, with some areas of high prevalence. In this context, the surveillance and control plans may need to be re-evaluated, and understanding the dynamics of bTB spread within Spanish herds may help to develop new strategies for reducing the time for detection of infected herds and for the elimination of bTB from the herds already infected. Here, we developed a compartmental stochastic model to simulate bTB within-herd transmission, fed it with epidemiological data from 22 herds (obtained from a previous work) and carried out parameter inference using Approximate Bayesian Computing methods We also estimated the "Within-herd transmission potential Number" (Rh), i...
January 31, 2018: Epidemics
Sara H Downs, Jessica E Parry, Paul A Upton, Jennifer M Broughan, Anthony V Goodchild, Javier Nuñez-Garcia, Matthias Greiner, Darrell A Abernethy, Angus R Cameron, Alasdair J Cook, Ricardo de la Rua-Domenech, Jane Gunn, Elizabeth Pritchard, Shelley Rhodes, Simon Rolfe, Michael Sharp, H Martin Vordermeier, Eamon Watson, Michael Welsh, Adam O Whelan, John A Woolliams, Simon J More, Richard S Clifton-Hadley
A systematic review was conducted to identify studies with data for statistical meta-analyses of sensitivity (Se) and specificity (Sp) of ante-mortem and post-mortem diagnostic tests for bovine tuberculosis (bTB) in cattle. Members of a working group (WG) developed and tested search criteria and developed a standardised two-stage review process, to identify primary studies with numerator and denominator data for test performance and an agreed range of covariate data. No limits were applied to year, language, region or type of test in initial searches of electronic databases...
November 9, 2017: Preventive Veterinary Medicine
S Tsairidou, A R Allen, R Pong-Wong, S H McBride, D M Wright, O Matika, C M Pooley, S W J McDowell, E J Glass, R A Skuce, S C Bishop, J A Woolliams
Genetic selection of cattle more resistant to bovine tuberculosis (bTB) may offer a complementary control strategy. Hypothesising underlying non-additive genetic variation, we present an approach using genome-wide high density markers to identify genomic loci with dominance effects on bTB resistance and to test previously published regions with heterozygote advantage in bTB. Our data comprised 1151 Holstein-Friesian cows from Northern Ireland, confirmed bTB cases and controls, genotyped with the 700K Illumina BeadChip...
January 24, 2018: Animal Genetics
Afroz S Mohammad, Jessica I Griffith, Chris E Adkins, Neal Shah, Emily Sechrest, Emma L Dolan, Tori B Terrell-Hall, Bart S Hendriks, Helen Lee, Paul R Lockman
PURPOSE: The blood-tumor barrier (BTB) limits irinotecan distribution in tumors of the central nervous system. However, given that the BTB has increased passive permeability we hypothesize that liposomal irinotecan would improve local exposure of irinotecan and its active metabolite SN-38 in brain metastases relative to conventional irinotecan due to enhanced-permeation and retention (EPR) effect. METHODS: Female nude mice were intracardially or intracranially implanted with human brain seeking breast cancer cells (brain metastases of breast cancer model)...
January 9, 2018: Pharmaceutical Research
Yunxia Liu, Jiwu Song, Jun Zhang, Liu Yang, Zongxia Liu, Xuxia Wang
OBJECTIVE: This study aimed to investigate the relationship between BTB/POZ domain-containing protein 7 (BTBD7) and fibronectin (FN) expression in salivary adenoid cystic carcinoma (SACC) and the function of BTBD7 in proliferation, migration, and invasion of SACC cells. STUDY DESIGN: The BTBD7 and FN expression in SACC and nontumor salivary tissues as well as SACC cells were characterized by immunohistochemistry and immunofluorescence. The effect of BTBD7 silencing on the proliferation, migration, and invasion of SACC-LM cells were determined by wound healing and transwell invasion assays...
December 28, 2017: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
A W Byrne, J Graham, C Brown, A Donaghy, M Guelbenzu-Gonzalo, J McNair, R A Skuce, A Allen, S W McDowell
Correctly identifying bovine tuberculosis (bTB) in cattle remains a significant problem in endemic countries. We hypothesized that animal characteristics (sex, age, breed), histories (herd effects, testing, movement) and potential exposure to other pathogens (co-infection; BVDV, liver fluke and Mycobacterium avium reactors) could significantly impact the immune responsiveness detected at skin testing and the variation in post-mortem pathology (confirmation) in bTB-exposed cattle. Three model suites were developed using a retrospective observational data set of 5,698 cattle culled during herd breakdowns in Northern Ireland...
January 23, 2018: Transboundary and Emerging Diseases
Anjali Misra, Thomas D McKnight, Kranthi K Mandadi
Global Transcription Factor Group E proteins GTE9 and GTE11 interact with BT2 to mediate ABA and sugar responses in Arabidopsis thaliana. BT2 is a BTB-domain protein that regulates responses to various hormone, stress and metabolic conditions in Arabidopsis thaliana. Loss of BT2 results in plants that are hypersensitive to inhibition of germination by abscisic acid (ABA) and sugars. Conversely, overexpression of BT2 results in resistance to ABA and sugars. Here, we report the roles of BT2-interacting partners GTE9 and GTE11, bromodomain and extraterminal-domain proteins of Global Transcription Factor Group E, in BT2-mediated responses to sugars and hormones...
January 23, 2018: Plant Molecular Biology
Hyun Ah Kim, Seong Gu Heo, Ji Wan Park, Young Ok Jung
Objective: The aim of this study was to identify the susceptibility genes responsible for lumbar spondylosis (LS) in Korean patients. Methods: Data from 1427 subjects were made available for radiographic grading and genome wide association studies (GWAS) analysis. Lateral lumbar spine radiographs were obtained and the various degrees of degenerative change were semi-quantitatively scored. A pilot GWAS was performed using the AffymetrixGenome-Wide Human single-nucleotide polymorphisms (SNPs), 500K array...
January 2018: Journal of Korean Neurosurgical Society
Baoming Liu, Debasis Panda, Jorge D Mendez-Rios, Sundar Ganesan, Linda S Wyatt, Bernard Moss
Genome uncoating is essential for replication of most viruses. For poxviruses, the process is divided into two stages: removal of the envelope allowing early gene expression, and breaching of the core wall allowing DNA release, replication and late gene expression. Subsequent studies showed that the host proteasome and the viral D5 protein, which has an essential role in DNA replication, are required for vaccinia virus (VACV) genome uncoating. In a search for additional VACV uncoating proteins, we noted a report that described a defect in DNA replication and late expression when the gene encoding a 68 kDa ankyrin-repeat/F box protein (68k-ank), associated with the cellular SCF ubiquitin ligase complex, was deleted from the attenuated modified vaccinia virus Ankara (MVA)...
January 17, 2018: Journal of Virology
Chenglv Hong, Xinlang Zhou, Weijian Huang, Peiren Shan, Fengquan Dong
A new microporous lanthanide metal-organic framework, {[Yb(BTB)(H2O) (DEF)2}n (1, DEF=N,N-Diethylformamide), with 1D nano-sized channels has been constructed by bridging helical chain secondary building units with 1,3,5-benzenetrisbenzoic acid (H3BTB) ligand. Structural characterization suggests that this complex crystallizes in the hexagonal space group P6122 and possesses 1D triangular channels with coordinated water molecules pointing to the channel center. In addition, anti-myocarditis properties of compound 1 were evaluated in vivo...
January 11, 2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
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