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https://www.readbyqxmd.com/read/28439067/perfluorooctanesulfonate-pfos-induced-sertoli-cell-injury-through-a-disruption-of-f-actin-and-microtubule-organization-is-mediated-by-akt1-2
#1
Ying Gao, Haiqi Chen, Xiang Xiao, Wing-Yee Lui, Will M Lee, Dolores D Mruk, C Yan Cheng
PFOS (perfluorooctanesulfonate, or perfluorooctane sulfonic acid) is an anthropogenic fluorosurfactant widely used in consumer products. While its use in Europe, Canada and the U.S. has been banned due to its human toxicity, it continues to be used in China and other developing countries as a global pollutant. Herein, using an in vitro model of Sertoli cell blood-testis barrier (BTB), PFOS was found to induce Sertoli cell injury by perturbing actin cytoskeleton through changes in the spatial expression of actin regulatory proteins...
April 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28438684/two-spliced-isoforms-of-the-sex-determination-gene-fruitless-in-the-chinese-mitten-crab-eriocheir-sinensis
#2
Peiyao Li, Yuan Liu, Danli Luo, Chengwen Song, Zhaoxia Cui
The fruitless (fru) gene plays an important role in sex determination pathway and courtship behavior of Drosophila melanogaster. In the present study, two fru isoforms (Esfru1 and Esfru2) were identified from the Chinese mitten crab Eriocheir sinensis. Sequence analysis showed that Esfru1 and Esfru2 were encoded by the same genomic locus and generated by alternative splicing of pre-mRNA. Esfru1 had all introns completely spliced out, while Esfru2 had a longer exon1 with an additional 78bp sequence. They both contained a conserved BTB domain which was also found in D...
April 21, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28426770/correction-risk-factors-for-bovine-tuberculosis-btb-in-cattle-in-ethiopia
#3
Sintayehu W Dejene, Ignas M A Heitkönig, Herbert H T Prins, Fitsum A Lemma, Daniel A Mekonnen, Zelalem E Alemu, Tessema Z Kelkay, Willem F de Boer
[This corrects the article DOI: 10.1371/journal.pone.0159083.].
2017: PloS One
https://www.readbyqxmd.com/read/28420366/pirfenidone-attenuates-bleomycin-induced-pulmonary-fibrosis-in-mice-by-regulating-nrf2-bach1-equilibrium
#4
Yuan Liu, Fuai Lu, Lirong Kang, Zhihua Wang, Yongfu Wang
BACKGROUND: Oxidative stress is one of the important factors involved in the pathogenesis of idiopathic pulmonary fibrosis (IPF). The equilibrium of Nuclear factor-erythroid-related factor 2 (Nrf2)/[BTB (broad-complex, tramtrack and bric-a-brac) and CNC (cap'n'collar protein) homology 1, Bach1] determines the expression level of antioxidant factors, further regulating the function of oxidation/antioxidation capacity. Pirfenidone (PFD) is one of two currently for IPF therapy approved drugs...
April 18, 2017: BMC Pulmonary Medicine
https://www.readbyqxmd.com/read/28413050/toll-like-receptor-2-gene-polymorphisms-in-chinese-holstein-cattle-and-their-associations-with-bovine-tuberculosis
#5
Zhanqin Zhao, Yun Xue, Zhigang Hu, Feng Zhou, Beibei Ma, Ta Long, Qiao Xue, Huisheng Liu
This study evaluated whether there was an association between polymorphisms within the Toll-like receptor 2 gene (TLR2) of Chinese Holstein cattle and susceptibility to bovine tuberculosis (BTB). In a case-control study including 210 BTB cases and 237 control cattle, we found only two common single-nucleotide polymorphisms (SNPs) within the entire coding region of the TLR2 gene, A631G (rs95214857) and T1707C (rs1388116488). Additionally, the allele and genotype distributions of A631G and T1707C were not different between case and control groups, indicated that these SNPs were not associated with susceptibility to BTB...
April 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28392519/detection-of-lipomannan-in-cattle-infected-with-bovine-tuberculosis
#6
Dung M Vu, Rama M Sakamuri, W Ray Waters, Basil I Swanson, Harshini Mukundan
Early and rapid detection of bovine tuberculosis (bTB) is critical to controlling the spread of this disease in cattle and other animals. In this study, we demonstrate the development of an immunoassay for the direct detection of the bovine bTB biomarker, lipomannan (LM) in serum using a waveguide-based optical biosensor. We apply an ultra-sensitive detection strategy developed by our team, termed lipoprotein capture, that exploits the pull-down of high-density lipoprotein (HDL) nanodiscs from cattle blood that allows for the recovery and detection of associated LM...
2017: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/28392087/evaluation-of-the-methodological-quality-of-studies-of-the-performance-of-diagnostic-tests-for-bovine-tuberculosis-using-quadas
#7
Sara H Downs, Simon J More, Anthony V Goodchild, Adam O Whelan, Darrell A Abernethy, Jennifer M Broughan, Angus Cameron, Alasdair J Cook, R Ricardo de la Rua-Domenech, Matthias Greiner, Jane Gunn, Javier Nuñez-Garcia, Shelley Rhodes, Simon Rolfe, Michael Sharp, Paul Upton, Eamon Watson, Michael Welsh, John A Woolliams, Richard S Clifton-Hadley, Jessica E Parry
There has been little assessment of the methodological quality of studies measuring the performance (sensitivity and/or specificity) of diagnostic tests for animal diseases. In a systematic review, 190 studies of tests for bovine tuberculosis (bTB) in cattle (published 1934-2009) were assessed by at least one of 18 reviewers using the QUADAS (Quality Assessment of Diagnostic Accuracy Studies) checklist adapted for animal disease tests. VETQUADAS (VQ) included items measuring clarity in reporting (n=3), internal validity (n=9) and external validity (n=2)...
March 22, 2017: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/28391861/a-flow-based-platform-for-measuring-the-acidity-parameters-in-wine
#8
Susana S M P Vidigal, António O S S Rangel
The present work describes a valuable tool for winemaking industry to measure the acidity parameters with rapid response, simple sample handling, with no or minimal pre-treatment. Thus, a sequential injection analysis (SIA) system with spectrophotometric detection was used as platform for the development of methodologies for the quantification of volatile and total acidity in wine samples. Both procedures make use of the same colour reagent, bromothymol blue (BTB) that changes its intrinsic colour in the presence of the acidity compounds...
June 1, 2017: Talanta
https://www.readbyqxmd.com/read/28382943/badger-macrophages-fail-to-produce-nitric-oxide-a-key-anti-mycobacterial-effector-molecule
#9
Kirstin Bilham, Amy C Boyd, Stephen G Preston, Christina D Buesching, Chris Newman, David W Macdonald, Adrian L Smith
The European badger is recognised as a wildlife reservoir for bovine tuberculosis (bTB); the control of which is complex, costly and controversial. Despite the importance of badgers in bTB and the well-documented role for macrophages as anti-mycobacterial effector cells, badger macrophage (bdMφ) responses remain uncharacterised. Here, we demonstrate that bdMφ fail to produce nitric oxide (NO) or upregulate inducible nitric oxide synthase (iNOS) mRNA following Toll-like receptor (TLR) agonist treatment. BdMφ also failed to make NO after stimulation with recombinant badger interferon gamma (bdIFNγ) or a combination of bdIFNγ and lipopolysaccharide...
April 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28379610/solid-state-nmr-proves-the-presence-of-penta-coordinated-sc-site-in-mil-100-sc
#10
Raynald Giovine, Christophe Volkringer, Sharon E Ashbrook, Julien Trébosc, David McKay, Thierry Loiseau, Jean-Paul Amoureux, Olivier Lafon, Frédérique Pourpoint
Advanced solid-state NMR methods and first-principles calculations demonstrate for the first time the formation of penta-coordinated scandium sites. These coordinatively unsatured sites were shown during the thermal activation of scandium-based Metal-Organic Frameworks (MOFs). ⁴⁵Sc NMR experiment allows their specific observation in activated Sc₃BTB₂ and MIL-100(Sc) MOFs. The assignment of the ScO₅ groups is supported by the DFT calculations of NMR parameters. The presence of ScO₅ Lewis acid sites in MIL-100(Sc) explains furthermore its catalytic activity...
April 5, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28372590/proteomic-analysis-of-protein-purified-derivative-of-mycobacterium-bovis
#11
Sante Roperto, Mariaconcetta Varano, Valeria Russo, Roberta Lucà, Monica Cagiola, Marco Gaspari, Dora Maria Ceccarelli, Giovanni Cuda, Franco Roperto
BACKGROUND: Tuberculin skin test based on in vivo intradermal inoculation of purified protein derivative from Mycobacterium bovis (bPPD) is the diagnostic test for the control and surveillance of bovine tuberculosis (bTB). METHODS: Proteomic analysis was performed on different bPPD preparations from M. bovis, strain AN5. Proteins were precipitated from bPPD solutions by TCA precipitation. The proteome of bPPD preparations was investigated by bottom-up proteomics, which consisted in protein digestion and nano-LC-MS/MS analysis...
April 3, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28365924/spatial-analysis-of-bovine-tuberculosis-in-the-state-of-mexico-mexico
#12
Adrian Zaragoza Bastida, Marivel Herna Ndez Tellez, Lilia Patricia Bustamante Montes, Jaime Nicola S Jaramillo Paniagua, Mari A Eugenia Jaimes Beni Tes, Germa N Eduardo Mendoza Barrera, Ninfa Rami Rez-Dura N
Bovine tuberculosis (bTB) is a chronic infectious disease that affects both domestic animals and wildlife. Veterinary epidemiology studies evaluate bTB using geographic information systems (GIS), which can characterise the spatial and temporal distribution of diseases and identify the geographic areas and animal populations at risk of contracting a disease. The present study used space‑time permutation scan statistic to identify the spatial and temporal distribution of bTB from 2005 to 2010 in the State of Mexico with the goal of creating a similarity model using Mahalanobis Distance to identify areas suitable for bTB occurrence...
March 31, 2017: Veterinaria Italiana
https://www.readbyqxmd.com/read/28364632/testicular-organoid-generation-by-a-novel-in%C3%A2-vitro-three-layer-gradient-system
#13
João Pedro Alves-Lopes, Olle Söder, Jan-Bernd Stukenborg
A system that models the testicular microenvironment and spermatogonial stem-cell (SSC) niche in vitro has not been produced yet. Here, we developed and characterized a novel three-dimensional multilayer model, the Three-Layer Gradient System (3-LGS), which permits the generation of rat testicular organoids with a functional blood-testis barrier (BTB) and germ cell establishment and proliferation. The model is unique as regards the formation of cellular organizations that more closely represent the in vivo germ-to-somatic cell associations in vitro...
June 2017: Biomaterials
https://www.readbyqxmd.com/read/28359669/anatomic-anterior-cruciate-ligament-reconstruction-via-independent-tunnel-drilling-a-systematic-review-of-randomized-controlled-trials-comparing-patellar-tendon-and-hamstring-autografts
#14
REVIEW
Michael C Ciccotti, Eric Secrist, Fotios Tjoumakaris, Michael G Ciccotti, Kevin B Freedman
PURPOSE: To collect the highest level of evidence comparing anatomic anterior cruciate ligament (ACL) reconstruction via independent tunnel drilling using bone-patellar tendon-bone (BTB) and hamstring tendon (HT) autografts in terms of clinical outcome and failure rate. METHODS: We performed a systematic review of clinical trials that randomized patients to ACL reconstruction with either BTB or HT autografts with a minimum 2-year follow-up. Only trials using independent tunnel drilling, including outside-in and anteromedial portal techniques, for both autografts were eligible for inclusion, whereas all transtibial studies were excluded...
March 27, 2017: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/28348866/integrated-computational-prediction-and-experimental-validation-identifies-promiscuous-t-cell-epitopes-in-the-proteome-of-mycobacterium-bovis
#15
Damien Farrell, Gareth Jones, Christopher Pirson, Kerri Malone, Kevin Rue-Albrecht, Anthony J Chubb, Martin Vordermeier, Stephen V Gordon
The discovery of novel antigens is an essential requirement in devising new diagnostics or vaccines for use in control programmes against human tuberculosis (TB) and bovine tuberculosis (bTB). Identification of potential epitopes recognised by CD4(+) T cells requires prediction of peptide binding to MHC class-II, an obligatory prerequisite for T cell recognition. To comprehensively prioritise potential MHC-II-binding epitopes from Mycobacterium bovis, the agent of bTB and zoonotic TB in humans, we integrated three binding prediction methods with the M...
August 2016: Microbial Genomics
https://www.readbyqxmd.com/read/28347519/meta-analyses-of-the-sensitivity-and-specificity-of-ante-mortem-and-post-mortem-diagnostic-tests-for-bovine-tuberculosis-in-the-uk-and-ireland
#16
Javier Nuñez-Garcia, Sara H Downs, Jessica E Parry, Darrell A Abernethy, Jennifer M Broughan, Angus R Cameron, Alasdair J Cook, Ricardo de la Rua-Domenech, Anthony V Goodchild, Jane Gunn, Simon J More, Shelley Rhodes, Simon Rolfe, Michael Sharp, Paul A Upton, H Martin Vordermeier, Eamon Watson, Michael Welsh, Adam O Whelan, John A Woolliams, Richard S Clifton-Hadley, Matthias Greiner
Bovine Tuberculosis (bTB) in cattle is a global health problem and eradication of the disease requires accurate estimates of diagnostic test performance to optimize their efficiency. The objective of this study was, through statistical meta-analyses, to obtain estimates of sensitivity (Se) and specificity (Sp), for 14 different ante-mortem and post-mortem diagnostic tests for bTB in cattle. Using data from a systematic review of the scientific literature (published 1934-2009) diagnostic Se and Sp were estimated using Bayesian logistic regression models adjusting for confounding factors...
March 6, 2017: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/28342861/microrna-548j-inhibits-type-i-interferon-production-by-targeting-zbtb11-in-patients-with-chronic-hepatitis-b
#17
Kangkang Yu, Qian Li, Qi Cheng, Chong Huang, Jianming Zheng, Shengsen Chen, Qingxia Ling, Mengqi Zhu, Mingquan Chen, Guangfeng Shi, Ning Li
Type I IFNs play crucial roles in antiviral immune responses. By inducing cellular resistance to viral infection and apoptosis of virus-infected cells, they impair virus replication and eliminate the invading pathogens. However, in CHB patients, generation of type I IFN was significantly impaired and the underlying mechanisms have not been fully understood. MicroRNA-548 family has been implicated in regulating antiviral response upon various infections. Here we explored the potential role of miR-548j in modulating type I IFN production in CHB...
March 22, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28339678/ddx4-egfp-transgenic-rat-model-for-the-study-of-germline-development-and-spermatogenesis-%C3%A2
#18
Kathrin Gassei, Yi Sheng, Adetunji Fayomi, Payal Mital, Meena Sukhwani, Chih-Cheng Lin, Karen A Peters, Andrew Althouse, Hanna Valli, Kyle E Orwig
Spermatogonial stem cells (SSC) are essential for spermatogenesis and male fertility. In addition, these adult tissue stem cells can be used as vehicles for germline modification in animal models and may have application for treating male infertility. To facilitate the investigation of SSCs and germ lineage development in rats, we generated a DEAD-box helicase 4 (DDX4) (VASA) promoter-enhanced green fluorescent protein (EGFP) reporter transgenic rat. Quantitative real-time polymerase chain reaction and immunofluorescence confirmed that EGFP was expressed in the germ cells of the ovaries and testes and was absent in somatic cells and tissues...
March 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28335717/genomic-regions-underlying-susceptibility-to-bovine-tuberculosis-in-holstein-friesian-cattle
#19
Kethusegile Raphaka, Oswald Matika, Enrique Sánchez-Molano, Raphael Mrode, Mike Peter Coffey, Valentina Riggio, Elizabeth Janet Glass, John Arthur Woolliams, Stephen Christopher Bishop, Georgios Banos
BACKGROUND: The significant social and economic loss as a result of bovine tuberculosis (bTB) presents a continuous challenge to cattle industries in the UK and worldwide. However, host genetic variation in cattle susceptibility to bTB provides an opportunity to select for resistant animals and further understand the genetic mechanisms underlying disease dynamics. METHODS: The present study identified genomic regions associated with susceptibility to bTB using genome-wide association (GWA), regional heritability mapping (RHM) and chromosome association approaches...
March 23, 2017: BMC Genetics
https://www.readbyqxmd.com/read/28333312/establishment-maintenance-and-functional-integrity-of-the-blood-testis-barrier-in-organotypic-cultures-of-fresh-and-frozen-thawed-prepubertal-mouse-testes
#20
C Rondanino, A Maouche, L Dumont, A Oblette, N Rives
STUDY QUESTION: Can the spatio-temporal formation of an intact blood-testis barrier (BTB), which is essential for the progression of spermatogenesis, be reproduced in cultures of fresh or frozen/thawed prepubertal mouse testes? SUMMARY ANSWER: Organotypic cultures allow the establishment and maintenance of major BTB components and the formation of a functional BTB in mouse testicular tissues. WHAT IS KNOWN ALREADY: In vitro maturation of prepubertal testicular tissues is a promising approach to restore fertility in adult survivors of childhood cancer...
March 17, 2017: Molecular Human Reproduction
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