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S Brubaker, K Lotherington, Jie Zhao, B Hamilton, G Rockl, A Duong, A Garibaldi, N Simunovic, D Alsop, D Dao, R Bessemer, O R Ayeni
For successful transplantation, allografts should be free of microorganisms that may cause harm to the allograft recipient. Before or during recovery and subsequent processing, tissues can become contaminated. Effective tissue recovery methods, such as minimizing recovery times (<24 h after death) and the number of experienced personnel performing recovery, are examples of factors that can affect the rate of tissue contamination at recovery. Additional factors, such as minimizing the time after asystole to recovery and the total time it takes to perform recovery, the type of recovery site, the efficacy of the skin prep performed immediately prior to recovery of tissue, and certain technical recovery procedures may also result in control of the rate of contamination...
October 19, 2016: Cell and Tissue Banking
Adolfo Paolin, Diletta Trojan, Antonio Carniato, Fabio Tasca, Ervino Massarin, Alessandro Tugnoli, Elisa Cogliati
Bacterial contamination of tissues retrieved from cadaveric donors is a common feature worldwide, and every tissue bank, albeit using different methods, conducts decontamination to guarantee safe tissues suitable for clinical use. The effectiveness of the methods used to eradicate pathogens differs. In order to reduce the tissue bioburden at retrieval, we have introduced a new method involving rinsing tissues in a sodium hypochlorite solution. To test its effectiveness we analyzed two comparable groups of tissues: Group A: 1881 tissues, all rinsed with isotonic saline solution after retrieval, and Group B: 1968 tissues immersed in an isotonic saline solution containing sodium hypochlorite (final concentration 0...
October 18, 2016: Cell and Tissue Banking
Shahzad Hussain, Zainab Khattak, Sidra Mahmood, Farnaz Malik, Humayun Riaz, Syed Atif Raza, Samiullah Khan
The microbial and chemical analysis of illicit drug samples from different areas of Pakistan i.e. Quetta, Karachi, Lahore and Islamabad was conducted in a cross-sectional study at National Institute of Health, Islamabad. The drug samples were confiscated by Anti Narcotics Force (ANF), Pakistan. Microbial analysis was done by estimating bioburden which revealed the presence of gram negative and positive bacteria's, fungus, Streptococcus, Staphylococcus species. Trypton soya agar was used for total aerobic count, MacConkey agar for gram-negative bacteria, Sabouraud dextrose agar for fungus and Vogel-Johnson agar for Streptococcus and Staphylococcus species...
September 2016: Pakistan Journal of Pharmaceutical Sciences
T L Landsman, T Touchet, S M Hasan, C Smith, B Russell, J Rivera, D J Maitland, E Cosgriff-Hernandez
: Uncontrolled hemorrhage accounts for more than 30% of trauma deaths worldwide. Current hemostatic devices focus primarily on time to hemostasis, but prevention of bacterial infection is also critical for improving survival rates. In this study, we sought to improve on current devices used for hemorrhage control by combining the large volume-filling capabilities and rapid clotting of shape memory polymer (SMP) foams with the swelling capacity of hydrogels. In addition, a hydrogel composition was selected that readily complexes with elemental iodine to impart bactericidal properties to the device...
October 6, 2016: Acta Biomaterialia
Ronja M Malinowski, Kasper W Lipsø, Mathilde H Lerche, Jan H Ardenkjær-Larsen
Signal enhancement by hyperpolarization is a way of overcoming the low sensitivity in magnetic resonance; MRI in particular. One of the most well-known methods, dissolution Dynamic Nuclear Polarization, has been used clinically in cancer patients. One way of ensuring a low bioburden of the hyperpolarized product is by use of a closed fluid path that constitutes a barrier to contamination. The fluid path can be filled with the pharmaceuticals, i.e. imaging agent and solvents, in a clean room, and then stored or immediately used at the polarizer...
September 22, 2016: Journal of Magnetic Resonance
X Triadó-Margarit, M Veillette, C Duchaine, M Talbot, F Amato, M C Minguillón, V Martins, E de Miguel, E O Casamayor, T Moreno
Subway systems worldwide transport more than 100 million people daily, therefore air quality on station platforms and inside trains is an important urban air pollution issue. We examined the microbiological composition and abundance in space and time of bioaerosols collected in the Barcelona subway system during a cold period. Quantitative PCR was used to quantify total bacteria, Aspergillus fumigatus, influenza A and B and rhinoviruses. Multitag 454 pyrosequencing of the 16S rRNA gene was used to assess bacterial community composition and biodiversity...
September 30, 2016: Indoor Air
Charles E Edmiston, David J Leaper
BACKGROUND: Intra-operative surgical site irrigation (lavage) is common practice in surgical procedures in general, with all disciplines advocating some form of irrigation before incision closure. This practice, however, has been neither standardized nor is there compelling evidence that it effectively reduces the risk of surgical site infection (SSI). This narrative review addresses the laboratory and clinical evidence that is available to support the practice of irrigation of the abdominal cavity and superficial/deep incisional tissues, using specific irrigation solutions at the end of an operative procedure to reduce the microbial burden at wound closure...
September 27, 2016: Surgical Infections
Mina Bashir, Mahjabeen Ahmed, Thomas Weinmaier, Doina Ciobanu, Natalia Ivanova, Thomas R Pieber, Parag A Vaishampayan
Strict planetary protection practices are implemented during spacecraft assembly to prevent inadvertent transfer of earth microorganisms to other planetary bodies. Therefore, spacecraft are assembled in cleanrooms, which undergo strict cleaning and decontamination procedures to reduce total microbial bioburden. We wanted to evaluate if these practices selectively favor survival and growth of hardy microorganisms, such as pathogens. Three geographically distinct cleanrooms were sampled during the assembly of three NASA spacecraft: The Lockheed Martin Aeronautics' Multiple Testing Facility during DAWN, the Kennedy Space Center's Payload Hazardous Servicing Facility (KSC-PHSF) during Phoenix, and the Jet Propulsion Laboratory's Spacecraft Assembly Facility during Mars Science Laboratory...
2016: Frontiers in Microbiology
J Mohr, M Germain, M Winters, S Fraser, A Duong, A Garibaldi, N Simunovic, D Alsop, D Dao, R Bessemer, O R Ayeni
Musculoskeletal allografts are typically disinfected using antibiotics, irradiation or chemical methods but protocols vary significantly between tissue banks. It is likely that different disinfection protocols will not have the same level of microorganism kill; they may also have varying effects on the structural integrity of the tissue, which could lead to significant differences in terms of clinical outcome in recipients. Ideally, a disinfection protocol should achieve the greatest bioburden reduction with the lowest possible impact on tissue integrity...
September 24, 2016: Cell and Tissue Banking
Vikram Bhatia, Anu Gupta, Shweta Sharma, Rajeev Shandil, Manav Wadhawan, Nitesh Agrawal, Ajay Kumar
OBJECTIVES: Endoscopic- ultrasound (EUS) aspiration needles are single-use devices. However, because of cost-constraints, these devices are reused multiple times in many centers. We studied microbiological contamination and bioburden on reprocessed needles, to evaluate if these devices can be sterilized. METHODS: We studied 10 EUS needles each, of 19G, 22G, and 25G size, and five 22G ProCore needles. Each needle was reprocessed by a standardized protocol, after the initial use...
September 16, 2016: Digestive Endoscopy: Official Journal of the Japan Gastroenterological Endoscopy Society
Thomas Serena, Heather Connell, Sharon McConnell, Keyur Patel, Bryan Doner, Matthew Sabo, Michael Miller, Laura Serena, Lam T Le, David Goldsmith, Jane Chung
OBJECTIVE: The purpose of this retrospective registry data analysis was to explore the effectiveness of a novel multivalent topical ointment (Terrasil Infection Control Wound Care Ointment; Aspiera Medical, Woonsocket, Rhode Island), containing a patented mineral complex and 0.2% benzethonium chloride in the treatment of nonhealing acute and chronic wounds. DESIGN: Aspiera Medical designed a registry to capture physician experiences and treatment results with Terrasil Infection Control Wound Care Ointment...
October 2016: Advances in Skin & Wound Care
Alicia M Shams, Laura J Rose, Jonathan R Edwards, Salvatore Cali, Anthony D Harris, Jesse T Jacob, Anna LaFae, Lisa L Pineles, Kerri A Thom, L Clifford McDonald, Matthew J Arduino, Judith A Noble-Wang
OBJECTIVE To determine the typical microbial bioburden (overall bacterial and multidrug-resistant organisms [MDROs]) on high-touch healthcare environmental surfaces after routine or terminal cleaning. DESIGN Prospective 2.5-year microbiological survey of large surface areas (>1,000 cm2). SETTING MDRO contact-precaution rooms from 9 acute-care hospitals and 2 long-term care facilities in 4 states. PARTICIPANTS Samples from 166 rooms (113 routine cleaned and 53 terminal cleaned rooms). METHODS Using a standard sponge-wipe sampling protocol, 2 composite samples were collected from each room; a third sample was collected from each Clostridium difficile room...
September 13, 2016: Infection Control and Hospital Epidemiology
Vita Boyar
BACKGROUND: Wound healing is a dynamic process that normally follows a predictable cascade of events. A common cause of delayed wound healing or wound dehiscence is increased colonization with microbes, often leading to infection. Infection may impede the healing process by inducing an undesirable inflammatory response. Systemic antibiotics and topical antiseptics are mainstays of treatment, but their adverse side effects and the potential for emergence of resistant microbial strains have led to a search for alternative approaches for control of bioburden...
September 2016: Journal of Wound, Ostomy, and Continence Nursing
Marino Ciliberti, Francesco De Lara, Gianfranco Serra, Felice Tafuro, Francesco Maria Iazzetta, Alessia Filosa, Rosa Scognamiglio, Giorgia Ciliberti, Maria Rosaria Veneri
OBJECTIVE: This study was designed to clinically evaluate the efficacy of a bacteria- and-fungi-binding mesh (BFBM) dressing to modify the bacterial load of pressure ulcers (PUs) of categories 3 and 4, when used as a wound contact layer (WCL) during negative pressure wound therapy (NPWT). METHODS: This was an observational single-centre study in patients with PUs of categories 3 or 4, who were treated with NPWT. Patients were observed for 7 days and received NPWT at -80 mm Hg with the BFBM dressing as the WCL...
August 15, 2016: Wounds: a Compendium of Clinical Research and Practice
Oleci P Frota, Adriano M Ferreira, Rodrigo Koch, Denise de Andrade, Marcelo A Rigotti, Najla M A Borges, Margarete T G Almeida
BACKGROUND: Cleaning of surfaces is essential in reducing environmental bioburdens and health care-associated infection in emergency units. However, there are few or no studies investigating cleaning surfaces in these scenarios. Our goal was to determine the influence of a multifaceted intervention on the effectiveness of routine cleaning of surfaces in a walk-in emergency care unit. METHODS: This prospective, before-and-after interventional study was conducted in 4 phases: phase I (situational diagnosis), phase II (implementation of interventions-feedback on results, standardization of cleaning procedures, and training of nursing staff), phase III (determination of the immediate influence of interventions), and phase IV (determination of the late influence of interventions)...
August 24, 2016: American Journal of Infection Control
Mayer Tenenhaus, Hans-Oliver Rennekampff
BACKGROUND: Pure regenerative healing with little to no donor morbidity remains an elusive goal for both surgeon and patient. The ability to engineer and promote the development of like tissue holds so much promise, and efforts in this direction are slowly but steadily advancing. METHODS: Products selected and reviewed reflect historical precedence and importance and focus on current clinically available products in use. Emerging technologies we anticipate will further expand our therapeutic options are introduced...
September 2016: Plastic and Reconstructive Surgery
(no author information available yet)
No abstract text is available yet for this article.
September 2016: Advances in Skin & Wound Care
Jeremy Honaker, Christie Ammons, Teresa Kelechi
PURPOSE: To provide information about initiating interdisciplinary research related to microbiomes and their role in human immunity, disease, and metabolic processes. TARGET AUDIENCE: This continuing education activity is intended for physicians and nurses with an interest in skin and wound care. OBJECTIVES: After participating in this educational activity, the participant should be better able to:1. Describe techniques to identify and characterize bacterial bioburden...
September 2016: Advances in Skin & Wound Care
Manu N Capoor, Filip Ruzicka, Tana Machackova, Radim Jancalek, Martin Smrcka, Jonathan E Schmitz, Marketa Hermanova, Jiri Sana, Elleni Michu, John C Baird, Fahad S Ahmed, Karel Maca, Radim Lipina, Todd F Alamin, Michael F Coscia, Jerry L Stonemetz, Timothy Witham, Garth D Ehrlich, Ziya L Gokaslan, Konstantinos Mavrommatis, Christof Birkenmaier, Vincent A Fischetti, Ondrej Slaby
BACKGROUND: The relationship between intervertebral disc degeneration and chronic infection by Propionibacterium acnes is controversial with contradictory evidence available in the literature. Previous studies investigating these relationships were under-powered and fraught with methodical differences; moreover, they have not taken into consideration P. acnes' ability to form biofilms or attempted to quantitate the bioburden with regard to determining bacterial counts/genome equivalents as criteria to differentiate true infection from contamination...
2016: PloS One
Andrew Gostine, David Gostine, Cristina Donohue, Luke Carlstrom
BACKGROUND: Ultraviolet (UV) spectrum light for decontamination of patient care areas is an effective way to reduce transmission of infectious pathogens. Our purpose was to investigate the efficacy of an automated UV-C device to eliminate bioburden on hospital computer keyboards. METHODS: The study took place at an academic hospital in Chicago, Illinois. Baseline cultures were obtained from keyboards in intensive care units. Automated UV-C lamps were installed over keyboards and mice of those computers...
October 1, 2016: American Journal of Infection Control
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