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Bioburden

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https://www.readbyqxmd.com/read/27912981/examining-the-association-between-surface-bioburden-and-frequently-touched-sites-in-intensive-care
#1
C E Adams, J Smith, V Watson, C Robertson, S J Dancer
BACKGROUND: Critical care patients are at increased risk of infection. Near-patient surfaces act as reservoirs of microbial soil, which may contain pathogens. AIM: To correlate soil levels with hand-touch frequency of near-patient sites in an intensive care unit (ICU). METHODS: Five sites around each bed in a 10-bed ICU were screened for total microbial soil (cfu/cm(2)) and Staphylococcus aureus every month for 10 months. Selected sites were infusion pump and cardiac monitor, left and right bedrails, and bed table...
November 13, 2016: Journal of Hospital Infection
https://www.readbyqxmd.com/read/27876528/the-impact-of-lens-care-solutions-on-corneal-epithelial-changes-during-daily-silicone-hydrogel-contact-lens-wear-as-measured-by-in-vivo-confocal-microscopy
#2
Xiaolin Zhang, Christine Marchetti, Jessica Lee, Yan Sun, Sara Debanne, Ying Jiang, Jami Kern, Mark Harrod, Beth Ann Benetz, Eric Pearlman, Loretta Szczotka-Flynn
PURPOSE: To assess corneal epithelial microstructure via confocal microscopy and determine if cellular changes are associated with lens care solutions during daily wear of silicone hydrogel contact lenses. METHODS: Corneal in vivo confocal microscopy with the Nidek ConfoScan4 was performed at baseline and after 5 months of lotrafilcon A daily contact lens wear. Enrolled participants were randomized to use either a polyhexamethylene biguanide (PHMB) preserved multipurpose care solution (MPS) or a peroxide based solution system...
November 19, 2016: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
https://www.readbyqxmd.com/read/27844457/application-of-the-atp-assay-to-rapidly-assess-cleanliness-of-spacecraft-surfaces-a-path-to-set-a-standard-for-future-missions
#3
James N Benardini, Kasthuri Venkateswaran
The National Aeronautics and Space Administration (NASA) measures and validates the biological cleanliness of spacecraft surfaces by counting endospores using the NASA standard assay (NSA). NASA has also approved an adenosine-5'-triphosphate (ATP)-based detection methodology as a means to prescreen surfaces for the presence of microbial contamination, prior to the spore assay. During Mars Science Laboratory (MSL) spacecraft assembly, test, and launch operations, 4853 surface samples were collected to verify compliance with the bioburden requirement at launch...
December 2016: AMB Express
https://www.readbyqxmd.com/read/27817762/improvement-of-hand-hygiene-quality-and-compliance-using-bioburden-measurement-and-online-feedback-in-germany
#4
Frank Günther, Kai Rudolph, Uwe Frank, Nico T Mutters
To improve compliance with hand hygiene, a novel method with inclusion of an online reporting system was developed, comprising measurement of total hand bioburden, anonymous online feedback, and onsite training. The intervention significantly improved both compliance and quality of hand hygiene and reduced Staphylococcus aureus incidence. Infect Control Hosp Epidemiol 2016;1-4.
November 7, 2016: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/27800587/evaluation-of-antibiotic-pressurized-pulse-lavage-for-contaminated-retromuscular-abdominal-wall-reconstruction
#5
Arnab Majumder, Heidi J Miller, Parita Patel, Yuhsin V Wu, Heidi L Elliott, Yuri W Novitsky
BACKGROUND: Despite patient risk factors such as diabetes and obesity, contamination during surgery remains a significant cause of infections and subsequent wound morbidity. Pressurized pulse lavage (PPL) has been utilized as a method to reduce bacterial bioburden with promising results in many fields. Although existing methods of lavage have been utilized during abdominal operations, no studies have examined the use of PPL during complex hernia repair. METHODS: Patients undergoing abdominal wall reconstruction (AWR) in clean-contaminated or contaminated fields with antibiotic PPL, from January 2012 to May 2013, were prospectively evaluated...
October 31, 2016: Surgical Endoscopy
https://www.readbyqxmd.com/read/27761677/tissue-recovery-practices-and-bioburden-a-systematic-review
#6
REVIEW
S Brubaker, K Lotherington, Jie Zhao, B Hamilton, G Rockl, A Duong, A Garibaldi, N Simunovic, D Alsop, D Dao, R Bessemer, O R Ayeni
For successful transplantation, allografts should be free of microorganisms that may cause harm to the allograft recipient. Before or during recovery and subsequent processing, tissues can become contaminated. Effective tissue recovery methods, such as minimizing recovery times (<24 h after death) and the number of experienced personnel performing recovery, are examples of factors that can affect the rate of tissue contamination at recovery. Additional factors, such as minimizing the time after asystole to recovery and the total time it takes to perform recovery, the type of recovery site, the efficacy of the skin prep performed immediately prior to recovery of tissue, and certain technical recovery procedures may also result in control of the rate of contamination...
December 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27757727/analysis-of-the-effectiveness-of-sodium-hypochlorite-decontamination-of-cadaveric-human-tissues-at-retrieval
#7
Adolfo Paolin, Diletta Trojan, Antonio Carniato, Fabio Tasca, Ervino Massarin, Alessandro Tugnoli, Elisa Cogliati
Bacterial contamination of tissues retrieved from cadaveric donors is a common feature worldwide, and every tissue bank, albeit using different methods, conducts decontamination to guarantee safe tissues suitable for clinical use. The effectiveness of the methods used to eradicate pathogens differs. In order to reduce the tissue bioburden at retrieval, we have introduced a new method involving rinsing tissues in a sodium hypochlorite solution. To test its effectiveness we analyzed two comparable groups of tissues: Group A: 1881 tissues, all rinsed with isotonic saline solution after retrieval, and Group B: 1968 tissues immersed in an isotonic saline solution containing sodium hypochlorite (final concentration 0...
December 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27731824/microbial-and-chemical-analysis-of-illicit-drugs-samples-confiscated-from-different-areas-of-pakistanmicrobial-and-chemical-analysis-of-illicit-drugs-samples-confiscated-from-different-areas-of-pakistan
#8
Shahzad Hussain, Zainab Khattak, Sidra Mahmood, Farnaz Malik, Humayun Riaz, Syed Atif Raza, Samiullah Khan
The microbial and chemical analysis of illicit drug samples from different areas of Pakistan i.e. Quetta, Karachi, Lahore and Islamabad was conducted in a cross-sectional study at National Institute of Health, Islamabad. The drug samples were confiscated by Anti Narcotics Force (ANF), Pakistan. Microbial analysis was done by estimating bioburden which revealed the presence of gram negative and positive bacteria's, fungus, Streptococcus, Staphylococcus species. Trypton soya agar was used for total aerobic count, MacConkey agar for gram-negative bacteria, Sabouraud dextrose agar for fungus and Vogel-Johnson agar for Streptococcus and Staphylococcus species...
September 2016: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27721009/a-shape-memory-foam-composite-with-enhanced-fluid-uptake-and-bactericidal-properties-as-a-hemostatic-agent
#9
T L Landsman, T Touchet, S M Hasan, C Smith, B Russell, J Rivera, D J Maitland, E Cosgriff-Hernandez
: Uncontrolled hemorrhage accounts for more than 30% of trauma deaths worldwide. Current hemostatic devices focus primarily on time to hemostasis, but prevention of bacterial infection is also critical for improving survival rates. In this study, we sought to improve on current devices used for hemorrhage control by combining the large volume-filling capabilities and rapid clotting of shape memory polymer (SMP) foams with the swelling capacity of hydrogels. In addition, a hydrogel composition was selected that readily complexes with elemental iodine to impart bactericidal properties to the device...
October 6, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27693965/dissolution-dynamic-nuclear-polarization-capability-study-with-fluid-path
#10
Ronja M Malinowski, Kasper W Lipsø, Mathilde H Lerche, Jan H Ardenkjær-Larsen
Signal enhancement by hyperpolarization is a way of overcoming the low sensitivity in magnetic resonance; MRI in particular. One of the most well-known methods, dissolution Dynamic Nuclear Polarization, has been used clinically in cancer patients. One way of ensuring a low bioburden of the hyperpolarized product is by use of a closed fluid path that constitutes a barrier to contamination. The fluid path can be filled with the pharmaceuticals, i.e. imaging agent and solvents, in a clean room, and then stored or immediately used at the polarizer...
September 22, 2016: Journal of Magnetic Resonance
https://www.readbyqxmd.com/read/27687789/bioaerosols-in-the-barcelona-subway-system
#11
X Triadó-Margarit, M Veillette, C Duchaine, M Talbot, F Amato, M C Minguillón, V Martins, E de Miguel, E O Casamayor, T Moreno
Subway systems worldwide transport more than 100 million people daily, therefore air quality on station platforms and inside trains is an important urban air pollution issue. We examined the microbiological composition and abundance in space and time of bioaerosols collected in the Barcelona subway system during a cold period. Quantitative PCR was used to quantify total bacteria, Aspergillus fumigatus, influenza A and B and rhinoviruses. Multitag 454 pyrosequencing of the 16S rRNA gene was used to assess bacterial community composition and biodiversity...
September 30, 2016: Indoor Air
https://www.readbyqxmd.com/read/27676639/intra-operative-surgical-irrigation-of-the-surgical-incision-what-does-the-future-hold-saline-antibiotic-agents-or-antiseptic-agents
#12
Charles E Edmiston, David J Leaper
BACKGROUND: Intra-operative surgical site irrigation (lavage) is common practice in surgical procedures in general, with all disciplines advocating some form of irrigation before incision closure. This practice, however, has been neither standardized nor is there compelling evidence that it effectively reduces the risk of surgical site infection (SSI). This narrative review addresses the laboratory and clinical evidence that is available to support the practice of irrigation of the abdominal cavity and superficial/deep incisional tissues, using specific irrigation solutions at the end of an operative procedure to reduce the microbial burden at wound closure...
September 27, 2016: Surgical Infections
https://www.readbyqxmd.com/read/27667984/functional-metagenomics-of-spacecraft-assembly-cleanrooms-presence-of-virulence-factors-associated-with-human-pathogens
#13
Mina Bashir, Mahjabeen Ahmed, Thomas Weinmaier, Doina Ciobanu, Natalia Ivanova, Thomas R Pieber, Parag A Vaishampayan
Strict planetary protection practices are implemented during spacecraft assembly to prevent inadvertent transfer of earth microorganisms to other planetary bodies. Therefore, spacecraft are assembled in cleanrooms, which undergo strict cleaning and decontamination procedures to reduce total microbial bioburden. We wanted to evaluate if these practices selectively favor survival and growth of hardy microorganisms, such as pathogens. Three geographically distinct cleanrooms were sampled during the assembly of three NASA spacecraft: The Lockheed Martin Aeronautics' Multiple Testing Facility during DAWN, the Kennedy Space Center's Payload Hazardous Servicing Facility (KSC-PHSF) during Phoenix, and the Jet Propulsion Laboratory's Spacecraft Assembly Facility during Mars Science Laboratory...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27665294/disinfection-of-human-musculoskeletal-allografts-in-tissue-banking-a-systematic-review
#14
REVIEW
J Mohr, M Germain, M Winters, S Fraser, A Duong, A Garibaldi, N Simunovic, D Alsop, D Dao, R Bessemer, O R Ayeni
Musculoskeletal allografts are typically disinfected using antibiotics, irradiation or chemical methods but protocols vary significantly between tissue banks. It is likely that different disinfection protocols will not have the same level of microorganism kill; they may also have varying effects on the structural integrity of the tissue, which could lead to significant differences in terms of clinical outcome in recipients. Ideally, a disinfection protocol should achieve the greatest bioburden reduction with the lowest possible impact on tissue integrity...
December 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27634338/residual-contamination-and-bioburden-after-reprocessing-of-single-use-endoscopic-ultrasound-needles-an-ex%C3%A2-vivo-study
#15
Vikram Bhatia, Anu Gupta, Shweta Sharma, Rajeev Shandil, Manav Wadhawan, Nitesh Agrawal, Ajay Kumar
BACKGROUND AND AIM: Endoscopic ultrasound (EUS) aspiration needles are single-use devices. However, in many centers, because of cost-constraints, these devices are reused multiple times. We studied microbiological contamination and bioburden on reprocessed needles to evaluate whether these devices can be successfully sterilized. METHODS: We studied 10 EUS needles each of 19 G, 22 G, and 25 G in size, and five 22-G ProCore needles. After initial use, each needle was reprocessed by a standardized protocol...
September 16, 2016: Digestive Endoscopy: Official Journal of the Japan Gastroenterological Endoscopy Society
https://www.readbyqxmd.com/read/27632443/novel-multivalent-wound-healing-ointment-provides-bioburden-control-and-moisture-management-a-retrospective-registry-data-analysis
#16
Thomas Serena, Heather Connell, Sharon McConnell, Keyur Patel, Bryan Doner, Matthew Sabo, Michael Miller, Laura Serena, Lam T Le, David Goldsmith, Jane Chung
OBJECTIVE: The purpose of this retrospective registry data analysis was to explore the effectiveness of a novel multivalent topical ointment (Terrasil Infection Control Wound Care Ointment; Aspiera Medical, Woonsocket, Rhode Island), containing a patented mineral complex and 0.2% benzethonium chloride in the treatment of nonhealing acute and chronic wounds. DESIGN: Aspiera Medical designed a registry to capture physician experiences and treatment results with Terrasil Infection Control Wound Care Ointment...
October 2016: Advances in Skin & Wound Care
https://www.readbyqxmd.com/read/27619507/assessment-of-the-overall-and-multidrug-resistant-organism-bioburden-on-environmental-surfaces-in-healthcare-facilities
#17
Alicia M Shams, Laura J Rose, Jonathan R Edwards, Salvatore Cali, Anthony D Harris, Jesse T Jacob, Anna LaFae, Lisa L Pineles, Kerri A Thom, L Clifford McDonald, Matthew J Arduino, Judith A Noble-Wang
OBJECTIVE To determine the typical microbial bioburden (overall bacterial and multidrug-resistant organisms [MDROs]) on high-touch healthcare environmental surfaces after routine or terminal cleaning. DESIGN Prospective 2.5-year microbiological survey of large surface areas (>1,000 cm2). SETTING MDRO contact-precaution rooms from 9 acute-care hospitals and 2 long-term care facilities in 4 states. PARTICIPANTS Samples from 166 rooms (113 routine cleaned and 53 terminal cleaned rooms). METHODS Using a standard sponge-wipe sampling protocol, 2 composite samples were collected from each room; a third sample was collected from each Clostridium difficile room...
December 2016: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/27607752/efficacy-of-dialkylcarbamoylchloride-coated-dressing-in-management-of-colonized-or-infected-neonatal-and-pediatric-wounds
#18
Vita Boyar
BACKGROUND: Wound healing is a dynamic process that normally follows a predictable cascade of events. A common cause of delayed wound healing or wound dehiscence is increased colonization with microbes, often leading to infection. Infection may impede the healing process by inducing an undesirable inflammatory response. Systemic antibiotics and topical antiseptics are mainstays of treatment, but their adverse side effects and the potential for emergence of resistant microbial strains have led to a search for alternative approaches for control of bioburden...
September 2016: Journal of Wound, Ostomy, and Continence Nursing
https://www.readbyqxmd.com/read/27589361/the-effect-of-a-bacteria-and-fungi-binding-mesh-dressing-on-the-bacterial-load-of-pressure-ulcers-treated-with-negative-pressure-wound-therapy-a-pilot-study
#19
Marino Ciliberti, Francesco De Lara, Gianfranco Serra, Felice Tafuro, Francesco Maria Iazzetta, Alessia Filosa, Rosa Scognamiglio, Giorgia Ciliberti, Maria Rosaria Veneri
OBJECTIVE: This study was designed to clinically evaluate the efficacy of a bacteria- and-fungi-binding mesh (BFBM) dressing to modify the bacterial load of pressure ulcers (PUs) of categories 3 and 4, when used as a wound contact layer (WCL) during negative pressure wound therapy (NPWT). METHODS: This was an observational single-centre study in patients with PUs of categories 3 or 4, who were treated with NPWT. Patients were observed for 7 days and received NPWT at -80 mm Hg with the BFBM dressing as the WCL...
November 2016: Wounds: a Compendium of Clinical Research and Practice
https://www.readbyqxmd.com/read/27566877/surface-cleaning-effectiveness-in-a-walk-in-emergency-care-unit-influence-of-a-multifaceted-intervention
#20
Oleci P Frota, Adriano M Ferreira, Rodrigo Koch, Denise de Andrade, Marcelo A Rigotti, Najla M A Borges, Margarete T G Almeida
BACKGROUND: Cleaning of surfaces is essential in reducing environmental bioburdens and health care-associated infection in emergency units. However, there are few or no studies investigating cleaning surfaces in these scenarios. Our goal was to determine the influence of a multifaceted intervention on the effectiveness of routine cleaning of surfaces in a walk-in emergency care unit. METHODS: This prospective, before-and-after interventional study was conducted in 4 phases: phase I (situational diagnosis), phase II (implementation of interventions-feedback on results, standardization of cleaning procedures, and training of nursing staff), phase III (determination of the immediate influence of interventions), and phase IV (determination of the late influence of interventions)...
December 1, 2016: American Journal of Infection Control
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