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https://www.readbyqxmd.com/read/28696230/implications-of-antimicrobial-combinations-in-complex-wound-biofilms-containing-fungi
#1
Eleanor M Townsend, Leighann Sherry, Ryan Kean, Donald Hansom, William G Mackay, Craig Williams, John Butcher, Gordon Ramage
Diabetic foot ulcer treatment currently focuses on targeting bacterial biofilms, while dismissing fungi. To investigate this we used an in vitro biofilm model containing bacteria and fungi, reflective of the wound environment, to test the impact of antimicrobials. Here we showed that while mono-treatment approaches influenced biofilm composition it had no discernible effect on overall quantity. Only by combining bacterial and fungal specific antibiotics were we able to decrease the biofilm bioburden, irrespective of composition...
July 10, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28684346/antibiotic-loaded-bone-graft-for-reduction-of-surgical-site-infection-in-spinal-fusion
#2
Stefanie M Shiels, Vivek P Raut, Patrick B Patterson, Brian R Barnes, Joseph C Wenke
BACKGROUND CONTEXT: Infections remain a leading complication associated with spinal arthrodesis, regardless of the use of prophylactic antibiotics and improved surgical techniques, with incidence of infection as high as 8.2%. Infection prolongs antibiotic usage, increases hospital time, and inevitably inflates overall treatment costs. Local antibiotics, such as vancomycin, have been used in combination with fusion materials over the past decade to decrease infection risk. An ideal graft material would serve a dual role: encourage vertebral fusion while reducing incidence of infection...
July 3, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28625700/the-effectiveness-of-sterilization-for-flexible-ureteroscopes-a-real-world-study
#3
Cori L Ofstead, Otis L Heymann, Mariah R Quick, Ellen A Johnson, John E Eiland, Harry P Wetzler
BACKGROUND: There are no guidelines or quality benchmarks specific to ureteroscope reprocessing, and patient injuries and infections have been linked to ureteroscopes. This prospective study evaluated ureteroscope reprocessing effectiveness. METHODS: Reprocessing practices at 2 institutions were assessed. Microbial cultures, biochemical tests, and visual inspections were conducted on sterilized ureteroscopes. RESULTS: Researchers examined 16 ureteroscopes after manual cleaning and sterilization using hydrogen peroxide gas...
May 4, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28624776/mold-control-and-detection-in-biological-drug-substance-manufacturing-facilities-an-industry-perspective
#4
Anita Bawa, Sophia Asefi, Stephanie Ramsey, Christine Arbesser-Rastburg, Mousumi Paul, Francisco Leira, Kim McFarland, Tracy Landeryou, Bindhu Reddy, Marie Murphy, Barbara Daddis, David Baine, Derek Willison-Parry
The biopharmaceutical industry produces non-sterile and/or low-bioburden intermediates and bulk biologics (i.e. Drug Substances) using bioburden controlled processes in accordance to Q7A and Annex 2. In many cases, single mold isolation events have received a high level of scrutiny; the goal of this paper is to challenge this paradigm and provide the rationale for an enhanced control approach that focuses on trending of mold species as microbial indicators rather than on single isolation events. Molds, can also be part (in much lower numbers) of the normal microbial population of a biologics manufacturing facility and, therefore, mold isolation is not an unexpected event in non-aseptic processing environments...
June 16, 2017: PDA Journal of Pharmaceutical Science and Technology
https://www.readbyqxmd.com/read/28602266/does-negative-pressure-wound-therapy-influence-subjacent-bacterial-growth-a-systematic-review
#5
REVIEW
Graeme E Glass, George R F Murphy, Jagdeep Nanchahal
BACKGROUND: Negative-pressure wound therapy is a ubiquitous wound management resource. The influence of NPWT on the bacterial bioburden of the subjacent wound remains unclear. We sought to examine the evidence. DATASOURCES: MEDLINE, Embase, PubMed, the Cochrane Database of Systematic Reviews and the Cochrane Controlled Trials Register were searched for articles quantitatively evaluating bacterial load under NPWT. RESULTS: Twenty-four studies met the inclusion criteria including 4 randomised controlled trials, 8 clinical series and 12 experimental studies...
May 22, 2017: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/28599034/a-prospective-randomized-controlled-two-arm-clinical-study-evaluating-the-efficacy-of-a-bioelectric-dressing-system-for-blister-management-in-us-army-ranger-recruits
#6
Greggory J Housler, Sue Cross, Vanessa Marcel, Daniel O Kennedy, Michael Husband, Andrew Register, Thomas Roberts, Seth Grubbs, Douglas Dudewicz, Nathan Setka, Curt Bay, Martin E Wendelken, Mina J Izadjoo
This study focused on a clinically relevant healthcare problem in the military: acute soft tissue wounds, or blisters. The trial was a prospective, controlled, randomized two-arm study evaluating the efficacy of a bioelectric dressing, Procellera®, applied topically two to three times per week for 2 weeks to blisters developed in Ranger trainees during training at Fort Benning, Georgia. A total of 80 US Army Ranger recruits with blister wounds below the knee were randomly assigned to one of two treatment groups (n = 40/group)...
2017: Journal of Special Operations Medicine: a Peer Reviewed Journal for SOF Medical Professionals
https://www.readbyqxmd.com/read/28594320/silver-containing-foam-dressings-with-safetac-a-review-of-the-scientific-and-clinical-data
#7
Phil Davies, Sara McCarty, Kristina Hamberg
Background Topical antimicrobials, such as silver dressings, are progressively being used alongside systemic antibiotics to provide adjunctive, antimicrobial therapy to wounds that are clinically infected or at risk of infection. To help improve wound management, dressings that use Safetac soft silicone technology in combination with a silver-impregnated foam dressing material were developed by Mölnlycke Health Care (Gothenburg, Sweden). The range comprises Mepilex Ag, Mepilex Border Ag and Mepilex Transfer Ag...
June 1, 2017: Journal of Wound Care
https://www.readbyqxmd.com/read/28575999/sterilization-of-silicone-based-hydrogels-for-biomedical-application-using-ozone-gas-comparison-with-conventional-techniques
#8
Raquel Galante, Daniela Ghisleni, Patrizia Paradiso, Vitor D Alves, Terezinha J A Pinto, Rogério Colaço, Ana Paula Serro
Sterilization of hydrogels is challenging due to their often reported sensitivity to conventional methods involving heat or radiation. Although aseptic manufacturing is a possibility, terminal sterilization is safer in biological terms, leading to a higher overall efficiency, and thus should be used whenever it is possible. The main goal of this work was to study the applicability of an innovative ozone gas terminal sterilization method for silicone-based hydrogels and compare its efficacy and effects with those of traditional sterilization methods: steam heat and gamma irradiation...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28570250/effect-of-negative-pressure-wound-therapy-with-instillation-on-bioburden-in-chronically-infected-wounds
#9
Chun Yang, Selena G Goss, Sean Alcantara, Greg Schultz, John C Lantis Ii
INTRODUCTION: Standard negative pressure wound therapy (NPWT) has been shown to help close wounds despite increasing planktonic bioburden. Both planktonic and biofilm critical colonization are associated with delayed wound healing; therefore, reducing microbial colonization is thought to aid wound healing. The use of NPWT with topical antimicrobial irrigation solution has previously shown reduction in quantitative planktonic bioburden when combined with sharp debridement in chronic wounds...
May 25, 2017: Wounds: a Compendium of Clinical Research and Practice
https://www.readbyqxmd.com/read/28566785/combined-negative-pressure-wound-therapy-with-open-bone-graft-for-infected-wounds-with-bone-defects-an-experimental-study
#10
Ramesh Kumar Jha, Chengyan Xia, Zonghuan Li, Weiyang Wang, Kai Deng
BACKGROUND: Bone and soft-tissue defects in infected wound have been an intractable problem to many surgical consultations. Infected wounds with bone defects are physical and financial burden to society. Nowadays, infected wounds with compound defect of bone and soft tissues are common in orthopedics department. Currently, no simple and efficient treatment has been found to solve this problem. This study investigates the effects of combining negative pressure wound therapy (NPWT) with open bone graft on this focus...
May 2017: Indian Journal of Orthopaedics
https://www.readbyqxmd.com/read/28522324/non-invasive-three-dimensional-imaging-of-escherichia-coli-k1-infection-using-diffuse-light-imaging-tomography-combined-with-micro-computed-tomography
#11
REVIEW
Luci A Witcomb, Julie Czupryna, Kevin P Francis, Gad Frankel, Peter W Taylor
In contrast to two-dimensional bioluminescence imaging, three dimensional diffuse light imaging tomography with integrated micro-computed tomography (DLIT-μCT) has the potential to realise spatial variations in infection patterns when imaging experimental animals dosed with derivatives of virulent bacteria carrying bioluminescent reporter genes such as the lux operon from the bacterium Photorhabdus luminescens. The method provides an opportunity to precisely localise the bacterial infection sites within the animal and enables the generation of four-dimensional movies of the infection cycle...
May 15, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28503756/biofilms-and-delayed-healing%C3%A2-%C3%A2-an-in-vitro-evaluation-of-silver-and-iodine-containing-dressings-and-their-effect-on-bacterial-and-human-cells
#12
Katie A Bourdillon, Craig P Delury, Breda M Cullen
This study investigated whether there are differences in the ability of wound dressings to modulate certain factors known to affect wound healing. A selection of antimicrobial dressings (AQUACEL(®) Ag Extra(™) , AQUACEL(®) Ag+ Extra(™) , IODOFLEX(™) , ACTICOAT(™) 7 and PROMOGRAN PRISMA(™) matrix) were tested for their effect on both bacterial bioburden and human dermal fibroblasts. Some dressings underwent further evaluation for activity against Pseudomonas aeruginosa biofilms using a colony-drip flow reactor model...
May 14, 2017: International Wound Journal
https://www.readbyqxmd.com/read/28480504/the-feasibility-of-gamma-radiation-sterilization-for-decellularized-tracheal-grafts
#13
Christopher M Johnson, DeHuang Guo, Stephanie Ryals, Gregory N Postma, Paul M Weinberger
OBJECTIVES/HYPOTHESIS: The most promising stem cell-derived tracheal transplantation approach is dependent upon the use of decellularized tracheal grafts. It has been assumed that a sterilization step, such as gamma radiation, would damage the delicate extracellular matrix of the graft, thus rendering it less viable for cellular repopulation, although this has not been thoroughly investigated. STUDY DESIGN: Laboratory-based comparative analysis. METHODS: Fifteen murine tracheas of strain C57/B-6 mice were obtained...
May 7, 2017: Laryngoscope
https://www.readbyqxmd.com/read/28475440/the-effect-of-negative-pressure-wound-therapy-with-antibacterial-dressings-or-antiseptics-on-an-in-vitro-wound-model
#14
J Matiasek, K J Domig, G Djedovic, R Babeluk, O Assadian
OBJECTIVE: The aim of this study was to investigate the bacterial bioburden in experimental in vitro wounds during the application of conventional negative pressure wound therapy (NPWT), with and without antimicrobial dressings (polyhexanide, silver), against NPWT instillation of octenidine. METHOD: Experimental wounds produced in an in vitro porcine wound model were homogenously contaminated with bacterial suspension and treated with NPWT and different options...
May 2, 2017: Journal of Wound Care
https://www.readbyqxmd.com/read/28473010/reduction-of-environmental-contamination-with-multidrug-resistant-bacteria-by-copper-alloy-coating-of-surfaces-in-a-highly-endemic-setting
#15
Maria Souli, Anastasia Antoniadou, Ioannis Katsarolis, Irini Mavrou, Elisabeth Paramythiotou, Evangelos Papadomichelakis, Maria Drogari-Apiranthitou, Theofano Panagea, Helen Giamarellou, George Petrikkos, Apostolos Armaganidis
OBJECTIVE To evaluate the efficacy of copper-coating in reducing environmental colonization in an intensive-care unit (ICU) with multidrug-resistant-organism (MDRO) endemicity DESIGN Interventional, comparative crossover trial SETTING The general ICU of Attikon University hospital in Athens, Greece PATIENTS Those admitted to ICU compartments A and B during the study period METHODS Before any intervention (phase 1), the optimum sampling method using 2 nylon swabs was validated. In phase 2, 6 copper-coated beds (ie, with coated upper, lower, and side rails) and accessories (ie, coated side table, intravenous [i...
May 5, 2017: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/28431851/ultraviolet-c-light-as-a-means-of-disinfecting-anesthesia-workstations
#16
Matthew Nottingham, Gene Peterson, Christopher Doern, Michelle Doll, Nadia Masroor, Kakotan Sanogo, Michael Stevens, Gonzalo Bearman
BACKGROUND: Anesthesia workstations (AWs) are a reservoir for pathogenic organisms potentially associated with surgical site infections. This study examined the effectiveness of the Tru-D SmartUVC device (Tru-D LLC, Nashville, TN) on bioburden reduction (BR) on AWs. METHODS: Strips of tissue inoculated with a known concentration of either Staphylococcus aureus, Enterococcus faecalis, or Acinetobacter sp were placed on 22 high-touch surfaces of an AW. Half of the AW surfaces received direct ultraviolet (UV) light exposure and half received indirect exposure...
April 18, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28416646/increasing-patient-safety-by-closing-the-sterile-production-gap-part-3-moist-heat-resistance-of-bioburden
#17
James P Agalloco
Terminal sterilization is considered the preferred means for the production of sterile drug products as it affords enhanced safety for the patient because the formulation is sterilized in its sealed, final container. Despite the obvious patient benefits, the use of terminal sterilization is artificially constrained by unreasonable expectations for the minimum time-temperature process to be used. The core misunderstanding with terminal sterilization is the notion that destruction of a high population of a resistant biological indicator microorganism is required...
April 17, 2017: PDA Journal of Pharmaceutical Science and Technology
https://www.readbyqxmd.com/read/28341262/pulsed-xenon-ultraviolet-light-disinfection-in-a-burn-unit-impact-on-environmental-bioburden-multidrug-resistant-organism-acquisition-and-healthcare-associated-infections
#18
Caroline Green, Jeremy C Pamplin, Kristine N Chafin, Clinton K Murray, Heather C Yun
Portable pulsed xenon ultraviolet disinfection (PPX-UVD) may reduce healthcare associated infections (HAI). There is limited data to inform use in burn intensive care units (BICU), where multidrug-resistant organisms (MDRO), especially gram negative rods (GNR), commonly cause disease. We evaluated PPX-UVD effects on environmental bioburden and rates of HAI and MDRO acquisition in a BICU. PPX-UVD was used for 3 months after standard cleaning of patient and operating rooms (ORs). Settle and touch plates in patient rooms and ORs were obtained after standard cleaning, pre-and post-PPX-UVD...
March 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/28336247/surgical-case-order-has-an-effect-on-the-risk-of-subsequent-periprosthetic-joint-infection
#19
Antonia F Chen, Michael M Kheir, Joshua M Greenbaum, Camilo Restrepo, Mitchell G Maltenfort, Javad Parvizi
BACKGROUND: Periprosthetic joint infection (PJI) after total joint arthroplasty (TJA) is a serious complication with multiple etiologies. Prior spine literature has shown that later cases in the day were more likely to develop surgical site infection. However, the effect of case order on PJI after TJA is unknown. This study aims to determine the influence of case order, prior infected case, and terminal cleaning on the risk for a subsequent PJI. METHODS: A retrospective, single-institution study was conducted on 31,499 TJAs performed from 2000 to 2014...
February 20, 2017: Journal of Arthroplasty
https://www.readbyqxmd.com/read/28333393/laser-generated-shockwaves-enhance-antibacterial-activity-against-biofilms-in-vitro
#20
William Yao, Edward C Kuan, Nathan C Francis, Maie A St John, Warren S Grundfest, Zachary D Taylor
BACKGROUND AND OBJECTIVES: Bacterial biofilm formation within chronic wound beds, which provides an effective barrier against antibiotics, is a known cause of recalcitrant infections and a significant healthcare burden, often requiring repeated surgical debridements. Laser-generated shockwaves (LGS) is a novel, minimally invasive, and nonthermal modality for biofilm mechanical debridement which utilizes compressive stress waves, generated by photonic absorption in thin titanium films to mechanically disrupt the biofilm...
March 23, 2017: Lasers in Surgery and Medicine
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