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https://www.readbyqxmd.com/read/29672134/metabolism-and-biodegradation-of-spacecraft-cleaning-reagents-by-strains-of-spacecraft-associated-acinetobacter
#1
Rakesh Mogul, Gregory A Barding, Sidharth Lalla, Sooji Lee, Steve Madrid, Ryan Baki, Mahjabeen Ahmed, Hania Brasali, Ivonne Cepeda, Trevor Gornick, Shawn Gunadi, Nicole Hearn, Chirag Jain, Eun Jin Kim, Thi Nguyen, Vinh Bao Nguyen, Alex Oei, Nicole Perkins, Joseph Rodriguez, Veronica Rodriguez, Gautam Savla, Megan Schmitz, Nicholas Tedjakesuma, Jillian Walker
Spacecraft assembly facilities are oligotrophic and low-humidity environments, which are routinely cleaned using alcohol wipes for benchtops and spacecraft materials, and alkaline detergents for floors. Despite these cleaning protocols, spacecraft assembly facilities possess a persistent, diverse, dynamic, and low abundant core microbiome, where the Acinetobacter are among the dominant members of the community. In this report, we show that several spacecraft-associated Acinetobacter metabolize or biodegrade the spacecraft cleaning reagents of ethanol (ethyl alcohol), 2-propanol (isopropyl alcohol), and Kleenol 30 (floor detergent) under ultraminimal conditions...
April 19, 2018: Astrobiology
https://www.readbyqxmd.com/read/29665443/activity-of-a-nitric-oxide-generating-wound-treatment-system-against-wound-pathogen-biofilms
#2
Richard D Waite, Joanne E Stewart, Abish S Stephen, Robert P Allaker
Wound bioburden plays an important role in impaired healing and the development of infection-related complications. The objective of this study was to determine the efficacy of an innovative two-layer nitric oxide generating system (NOx) to prevent and treat biofilms formed by bacterial and fungal pathogens commonly associated with wound infection, and activity against Pseudomonas aeruginosa virulence factors. Single and mixed species biofilms were grown for 24h on nitrocellulose filters placed on agar. Filters were covered with either NOx or placebo, before and after biofilm formation...
April 14, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29651276/liposome-entrapment-of-bacteriophages-improves-wound-healing-in-a-diabetic-mouse-mrsa-infection
#3
Sanjay Chhibber, Jasjeet Kaur, Sandeep Kaur
Diabetic populations are more prone to developing wound infections which results in poor and delayed wound healing. Infection with drug resistant organisms further worsen the situation, driving searches for alternative treatment approaches such as phage therapy. Major drawback of phage therapy, however, is low phage persistence in situ , suggesting further refinement of the approach. In the present work we address this issue by employing liposomes as delivery vehicles. A liposome entrapped phage cocktail was evaluated for its ability to resolve a Staphylococcus aureus -induced diabetic excission wound infection...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29649556/is-there-a-relationship-between-airborne-and-surface-microbes-in-the-critical-care-environment
#4
J Smith, C A Adams, M F King, C J Noakes, C Robertson, S J Dancer
OBJECTIVE: This study attempted firstly to correlate environmental contamination of air and surfaces in the intensive care unit (ICU); and secondly, to examine any association between environmental contamination and ICU-acquired staphylococcal infection. DESIGN: We screened patients, air and surfaces on 10 sampling days in a mechanically ventilated 10-bed ICU during 10 months. METHODS: Near-patient hand-touch sites (n=500) and air (n=80) were screened for total colony count and Staphylococcus aureus using dipslides, settle plates (passive air sampling) and an MAS-100 slit-sampler (active air sampling)...
April 9, 2018: Journal of Hospital Infection
https://www.readbyqxmd.com/read/29644090/human-bone-graft-cytocompatibility-with-mesenchymal-stromal-cells-is-comparable-after-thermal-sterilization-and-washing-followed-by-%C3%AE-irradiation-an-in-vitro-study
#5
Dmitry Labutin, Konstantin Vorobyov, Svetlana Bozhkova, Ekaterina Polyakova, Tatyana Vodopyanova
Human bone allografts present a better alternative to autografts in terms of minimization of the harvesting procedure complications. Prior to the use in clinical applications, they require sterilization which aims to reduce bioburden. This often comes at the expense of their biological properties as carriers of cells. In this study, we evaluated the cytocompatibility of human bone allografts processed and sterilized by three different methods with mesenchymal stromal cells. Bone morphology, biological and biochemical properties of the extracted bone-conditioned medium and viability of cells were assessed...
March 2018: Regenerative Biomaterials
https://www.readbyqxmd.com/read/29623137/assessing-a-novel-method-to-reduce-anesthesia-machine-contamination-a-prospective-observational-trial
#6
Chuck J Biddle, Beverly George-Gay, Praveen Prasanna, Emily M Hill, Thomas C Davis, Brad Verhulst
Background: Anesthesia machines are known reservoirs of bacterial species, potentially contributing to healthcare associated infections (HAIs). An inexpensive, disposable, nonpermeable, transparent anesthesia machine wrap (AMW) may reduce microbial contamination of the anesthesia machine. This study quantified the density and diversity of bacterial species found on anesthesia machines after terminal cleaning and between cases during actual anesthesia care to assess the impact of the AMW...
2018: Canadian Journal of Infectious Diseases & Medical Microbiology
https://www.readbyqxmd.com/read/29609854/residual-moisture-and-waterborne-pathogens-inside-flexible-endoscopes-evidence-from-a-multisite-study-of-endoscope-drying-effectiveness
#7
Cori L Ofstead, Otis L Heymann, Mariah R Quick, John E Eiland, Harry P Wetzler
BACKGROUND: Endoscopy-associated infection transmission is frequently linked to inadequate reprocessing. Residual organic material and moisture may foster biofilm development inside endoscopes. This study evaluated the effectiveness of endoscope drying and storage methods and assessed associations between retained moisture and contamination. METHODS: Endoscope reprocessing, drying, and storage practices were assessed at 3 hospitals. Researchers performed visual examinations and tests to detect fluid and contamination on patient-ready endoscopes...
March 30, 2018: American Journal of Infection Control
https://www.readbyqxmd.com/read/29604580/filters-from-taxis-air-conditioning-system-a-tool-to-characterize-driver-s-occupational-exposure-to-bioburden
#8
Carla Viegas, Ana Monteiro, Mateus Dos Santos, Tiago Faria, Liliana Aranha Caetano, Elisabete Carolino, Anita Quintal Gomes, Geneviève Marchand, Nancy Lacombe, Susana Viegas
Bioburden proliferation in filters from air conditioning systems of taxis represents a possible source of occupational exposure. The aim of this study was to determine the occurrence of fungi and bacteria in filters from the air conditioning system of taxis used for patient transportation and to assess the exposure of drivers to bioburden. Filters from the air conditioning systems of 19 taxis and 28 personal vehicles (used as controls) operating in three Portuguese cities including the capital Lisbon, were collected during the winter season...
March 28, 2018: Environmental Research
https://www.readbyqxmd.com/read/29593560/intestinal-metagenomes-and-metabolomes-in-healthy-young-males-inactivity-and-hypoxia-generated-negative-physiological-symptoms-precede-microbial-dysbiosis
#9
Robert Šket, Tadej Debevec, Susanne Kublik, Michael Schloter, Anne Schoeller, Boštjan Murovec, Katarina Vogel Mikuš, Damjan Makuc, Klemen Pečnik, Janez Plavec, Igor B Mekjavić, Ola Eiken, Zala Prevoršek, Blaž Stres
We explored the metagenomic, metabolomic and trace metal makeup of intestinal microbiota and environment in healthy male participants during the run-in (5 day) and the following three 21-day interventions: normoxic bedrest (NBR), hypoxic bedrest (HBR) and hypoxic ambulation (HAmb) which were carried out within a controlled laboratory environment (circadian rhythm, fluid and dietary intakes, microbial bioburden, oxygen level, exercise). The fraction of inspired O2 (Fi O2 ) and partial pressure of inspired O2 (Pi O2 ) were 0...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29561341/the-management-of-critically-colonized-and-locally-infected-leg-ulcers-with-an-acid-oxidizing-solution-a-pilot-study
#10
Robert Strohal, Martina Mittlböck, Gilbert Hämmerle
OBJECTIVE: Critical colonization or local infection is very common in chronic wounds, but clinically problematic. Because therapeutic options for these conditions are limited in number and efficacy, the study authors tested a new acid-oxidizing solution (AOS [Nexodyn]; APR Applied Pharma Research S.A., Balerna, Switzerland) to determine its ancillary antimicrobial properties and potential support for wound healing. DESIGN AND SETTING: This open-label clinical case series was conducted with a prospective, single-arm design at the Federal County Hospital in Bregenz, Austria...
April 2018: Advances in Skin & Wound Care
https://www.readbyqxmd.com/read/29556882/bioburden-in-transport-solutions-of-human-cardiovascular-tissues-a-comparative-evaluation-of-direct-inoculation-and-membrane-filter-technique
#11
Letícia Kraft, Victoria Stadler Tasca Ribeiro, Luciana Cristina Wollmann, Paula Hansen Suss, Felipe Francisco Tuon
All cardiac allograft tissues are under potential contamination, requiring a validated terminal sterilization process or a minimal bioburden. The bioburden calculation is important to determine the bacterial burden and further decontamination and disinfection strategies for the valve processing. The aim of this study was to determine the bioburden from transport solution (TS) of heart valves obtained from non-heart-beating and heart-beating donors in different culture methods. The bioburden from TS was determined in 20 hearts donated for valve allograft tissue using membrane filter (MF) and direct inoculation...
March 19, 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29485016/comparing-the-bioburden-measured-by-adenosine-triphosphate-atp-luminescence-technology-to-contact-plate-based-microbiologic-sampling-to-assess-the-cleanliness-of-the-patient-care-environment
#12
Elizabeth Salsgiver, Daniel Bernstein, Matthew S Simon, William Greendyke, Haomiao Jia, Amy Robertson, Selma Salter, Audrey N Schuetz, Lisa Saiman, E Yoko Furuya, David P Calfee
The correlation between ATP concentration and bacterial burden in the patient care environment was assessed. These findings suggest that a correlation exists between ATP concentration and bacterial burden, and they generally support ATP technology manufacturer-recommended cutoff values. Despite relatively modest discriminative ability, this technology may serve as a useful proxy for cleanliness. Infect Control Hosp Epidemiol 2018;1-3.
February 27, 2018: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/29471796/impact-of-pulsed-xenon-ultraviolet-disinfection-on-surface-contamination-in-a-hospital-facility-s-expressed-human-milk-feed-preparation-area
#13
Ricky Dippenaar, Johan Smith
BACKGROUND: Expressed human milk (EHM) feed preparation areas represent a potential source of unintentional nosocomial infection. Daily disinfection of environmental surfaces remains an essential intervention to mitigate nosocomial infections. The inefficiency of conventional cleaning and disinfection contributes to an increased risk for the acquisition of multi-drug resistant pathogens. "Non touch" technologies such as the pulsed xenon ultraviolet (PX-UVD) light device have documented sustained reduction in surface bacterial colonization and reduced cross contamination...
February 23, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29444992/microbiological-control-for-affinity-capture-chromatography-processing-an-industry-perspective
#14
Marie Murphy, Brian Bell, James Moshi, Robert Grassi, Ren-Yo Forng, Angel Salaman, Petra Wawra Jordi, Simin Zaidi, Marcella Goodnight, Kim McFarland, Diane Hardy, Michael Knight, Mousumi Paul, Andreas Keller, Bill Carpenter, Donal McLaughlin, Una Hearty, Jose Marrero, David Bain, Derek Willison-Parry
The purpose of this paper is to provide a summary of a BPOG-led industry survey of the microbiological control aspects of affinity chromatography processing in the biopharmaceutical industry. The document provides a summary of historical microbiological control concerns, coupled with industry-derived best practices for material, equipment, and storage controls required to mitigate the potential for microbial ingress and contamination of chromatography resin and equipment. These best practice guidelines, which are derived from the members of the BPOG Bioburden Working Group, are intended to assist biopharmaceutical manufacturers enhance microbial control and monitoring strategies for chromatography systems...
February 14, 2018: PDA Journal of Pharmaceutical Science and Technology
https://www.readbyqxmd.com/read/29428003/how-does-a-photocatalytic-antimicrobial-coating-affect-environmental-bioburden-in-hospitals
#15
Matthew Reid, Vanessa Whatley, Emma Spooner, Alan M Nevill, Michael Cooper, Jeremy J Ramsden, Stephanie J Dancer
BACKGROUND The healthcare environment is recognized as a source for healthcare-acquired infection. Because cleaning practices are often erratic and always intermittent, we hypothesize that continuously antimicrobial surfaces offer superior control of surface bioburden. OBJECTIVE To evaluate the impact of a photocatalytic antimicrobial coating at near-patient, high-touch sites in a hospital ward. SETTING The study took place in 2 acute-care wards in a large acute-care hospital. METHODS A titanium dioxide-based photocatalytic coating was sprayed onto 6 surfaces in a 4-bed bay in a ward and compared under normal illumination against the same surfaces in an untreated ward: right and left bed rails, bed control, bedside locker, overbed table, and bed footboard...
February 12, 2018: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/29363971/design-and-syntheses-of-highly-potent-teixobactin-analogues-against-staphylococcus-aureus-methicillin-resistant-staphylococcus-aureus-mrsa-and-vancomycin-resistant-enterococci-vre-in-vitro-and-in-vivo
#16
Anish Parmar, Rajamani Lakshminarayanan, Abhishek Iyer, Venkatesh Mayandi, Eunice Tze Leng Goh, Daniel G Lloyd, Madhavi Latha S Chalasani, Navin K Verma, Stephen H Prior, Roger W Beuerman, Annemieke Madder, Edward J Taylor, Ishwar Singh
The cyclic depsipeptide, teixobactin, kills a number of Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA), and Mycobacterium tuberculosis without detectable resistance. To date, teixobactin is the only molecule in its class that has shown in vivo antibacterial efficacy. In this work, we designed and synthesized 10 new in vivo ready teixobactin analogues. These analogues showed highly potent antibacterial activities against Staphylococcus aureus, MRSA, and vancomycin-resistant enterococci (VRE) in vitro...
March 8, 2018: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29350654/autolytic-debridement-and-management-of-bacterial-load-with-an-occlusive-hydroactive-deressing-impregnated-with-polyhexamethylene-biguanide
#17
Stefano Mancini, Roberto Cuomo, Mauro Poggialini, Carlo D'Aniello, Giuseppe Botta
BACKGROUND: Debridement and control of bacterial load are key-points of wound care. The aim of this study is to evaluate the effectiveness of autolytic debridement and management of bacterial load (bioburden) of an occlusive hydro-active dressing impregnated with polyhexamethylene biguanide (PHMB). METHODS: We used an occlusive dressing of hydro-active for leg ulcers' treatment (TenderWet-plus® - Hartmann).  Inclusion criteria were: presence of a fibrinous ulcer with multiple etiopathogenesis, in absence of clinical infection, no hypersecretion and diameter within 5 cm...
January 16, 2018: Acta Bio-medica: Atenei Parmensis
https://www.readbyqxmd.com/read/29280976/a-novel-multi-approach-protocol-for-the-characterization-of-occupational-exposure-to-organic-dust-swine-production-case-study
#18
Carla Viegas, Tiago Faria, Ana Monteiro, Liliana Aranha Caetano, Elisabete Carolino, Anita Quintal Gomes, Susana Viegas
Swine production has been associated with health risks and workers' symptoms. In Portugal, as in other countries, large-scale swine production involves several activities in the swine environment that require direct intervention, increasing workers' exposure to organic dust. This study describes an updated protocol for the assessment of occupational exposure to organic dust, to unveil an accurate scenario regarding occupational and environmental risks for workers' health. The particle size distribution was characterized regarding mass concentration in five different size ranges (PM0...
December 27, 2017: Toxics
https://www.readbyqxmd.com/read/29178633/norfloxacin-salts-of-carboxylic-acids-curtail-planktonic-and-biofilm-mode-of-growth-in-eskape-pathogens
#19
R C Lowrence, A Ramakrishnan, N S Sundaramoorthy, A Shyam, V Mohan, H M V Subbarao, V Ulaganathan, T Raman, A Solomon, S Nagarajan
AIMS: To enhance the antimicrobial and antibiofilm activity of norfloxacin against the planktonic and biofilm mode of growth in ESKAPE pathogens using chemically modified norfloxacin salts. METHODS AND RESULTS: Antimicrobial testing, synergy testing and time-kill curve analysis were performed to evaluate antibacterial effect of norfloxacin carboxylic acid salts against ESKAPE pathogens. In vivo efficacy to reduce bacterial bioburden was evaluated in zebrafish infection model...
February 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29166254/proactive-and-early-aggressive-wound-management-a-shift-in-strategy-developed-by-a-consensus-panel-examining-the-current-science-prevention-and-management-of-acute-and-chronic-wounds
#20
Gregory A Bohn, Gregory S Schultz, Brock A Liden, Michael N Desvigne, Eric J Lullove, Igor Zilberman, Mary B Regan, Marta Ostler, Karen Edwards, Georgia M Arvanitis, Jodi F Hartman
Normal wound healing is accomplished through a series of well-coordinated, progressive events with overlapping phases. Chronic wounds are described as not progressing to healing or not being responsive to management in a timely manner. A consensus panel of multidisciplinary wound care professionals was assembled to (1) educate wound care practitioners by identifying key principles of the basic science of chronic wound pathophysiology, highlighting the impact of metalloproteinases and biofilms, as well as the role of the extracellular matrix; and (2) equip practitioners with a systematic strategy for the prevention and healing of acute injuries and chronic wounds based upon scientific evidence and the panel members' expertise...
November 2017: Wounds: a Compendium of Clinical Research and Practice
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