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RND efflux pump

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https://www.readbyqxmd.com/read/29760777/a-pseudomonas-putida-efflux-pump-acts-on-short-chain-alcohols
#1
Georg Basler, Mitchell Thompson, Danielle Tullman-Ercek, Jay Keasling
Background: The microbial production of biofuels is complicated by a tradeoff between yield and toxicity of many fuels. Efflux pumps enable bacteria to tolerate toxic substances by their removal from the cells while bypassing the periplasm. Their use for the microbial production of biofuels can help to improve cell survival, product recovery, and productivity. However, no native efflux pump is known to act on the class of short-chain alcohols, important next-generation biofuels, and it was considered unlikely that such an efflux pump exists...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29740005/nitrothiophene-carboxamides-a-novel-narrow-spectrum-antibacterial-series-mechanism-of-action-and-efficacy
#2
Shahul Hameed P, Nagakumar Bharatham, Nainesh Katagihallimath, Sreevalli Sharma, Radha Nandishaiah, Anirudh P Shanbhag, Teby Thomas, Riya Narjari, Maitrayee Sarma, Purnendu Bhowmik, Prakruthi Amar, Rajani Ravishankar, Ramesh Jayaraman, Kubendran Muthan, Ramesh Subbiah, Vasanthi Ramachandran, V Balasubramanian, Santanu Datta
The mechanism of efflux is a tour-de-force in the bacterial armoury that has thwarted the development of novel antibiotics. We report the discovery of a novel chemical series with potent antibacterial properties that was engineered to overcome efflux liability. Compounds liable to efflux specifically via the Resistance Nodulation and cell Division (RND) pump, AcrAB-TolC were chosen for a hit to lead progression. Using structure-based design, the compounds were optimised to lose their binding to the efflux pump, thereby making them potent on wild-type bacteria...
May 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29725253/anti-biofilm-and-anti-virulence-efficacy-of-celastrol-against-stenotrophomonas-maltophilia
#3
Hye-Rim Kim, Dongsup Lee, Yong-Bin Eom
Stenotrophomonas maltophilia is a multi-drug resistant opportunistic pathogen that causes nosocomial infections in immunocompromised patients. This pathogen is difficult to treat owing to its intrinsic multidrug resistance and ability to form antimicrobial-tolerant biofilms. In the present study, we aimed to assess the potential use of celastrol as a novel anti-biofilm and/or anti-virulence agent against S. maltophilia . Results showed that celastrol at its sub-inhibitory doses decreased biofilm formation and disrupt the established biofilms produced by S...
2018: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29687766/identification-and-prevalence-of-rnd-family-multidrug-efflux-pump-oqxab-genes-in-enterococci-isolates-from-swine-manure-in-china
#4
Li Yuan, Ya-Jun Zhai, Hua Wu, Hua-Run Sun, Zhi-Pei He, Ya-Bin Wang, Yu-Shan Pan, Nan-Nan Kuang, Gong-Zheng Hu
PURPOSE: The resistance/nodulation/cell division (RND) family multidrug efflux pump, OqxAB, has been identified as one of the leading mechanisms of plasmid-mediated quinolone resistance and has become increasingly prevalent among Enterobacteriaceae in recent years. However, oqxAB genes have not yet been reported in Enterococcus isolates. The aim of the present study was to identify the oqxAB genes and investigate their prevalence among Enterococcus from swine manure in China. METHODOLOGY: The oqxAB genes were screened in 87 Enterococcus isolates by PCR...
April 23, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29661860/substrate-specificities-and-efflux-efficiencies-of-rnd-efflux-pumps-of-acinetobacter-baumannii
#5
Inga V Leus, Jon W Weeks, Vincent Bonifay, Lauren Smith, Sophie Richardson, Helen I Zgurskaya
Antibiotic resistant Acinetobacter baumannii causes infections that are extremely difficult to treat. A significant role in these resistance profiles is attributed to multidrug efflux pumps, especially those belonging to Resistance-Nodulation-cell Division (RND) superfamily of transporters. In this study, we analyzed functions and properties of RND efflux pumps in A. baumannii ATCC 17978. This strain is susceptible to antibiotics and does not contain mutations that are commonly selected upon exposure to high concentrations of antibiotics...
April 16, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29625653/the-role-of-efflux-pumps-in-bacteroides-fragilis-resistance-to-antibiotics
#6
REVIEW
Reza Ghotaslou, Mina Yekani, Mohammad Yousef Memar
The resistance of Bacteroides fragilis to the most antimicrobial agents has been reported in the world. Identification of the microbial resistance mechanisms can play an important role in controlling these resistances. Currently, B. fragilis is resistant to most antibiotics. The multi-drug efflux pumps have been shown to underlie the antimicrobial resistance in B. fragilis strains. Two types of these efflux pumps including RND and MATE can be regarded as main structures responsible for antibiotic resistance...
May 2018: Microbiological Research
https://www.readbyqxmd.com/read/29577985/rnd-transporters-in-the-living-world
#7
Hiroshi Nikaido
Transporters of the RND superfamily are well-known as the major drug efflux pumps of Gram-negative bacteria. However, they are widespread in organisms ranging from Archaea to Eukaryotes, and perform diverse functions. This review gives a brief overview of these diverse members of the superfamily with emphasis on their structure and functions.
March 22, 2018: Research in Microbiology
https://www.readbyqxmd.com/read/29554339/diversity-of-mutations-in-regulatory-genes-of-resistance-nodulation-cell-division-efflux-pumps-in-association-with-tigecycline-resistance-in-acinetobacter-baumannii
#8
Stefanie Gerson, Jennifer Nowak, Esther Zander, Julia Ertel, Yurong Wen, Oleg Krut, Harald Seifert, Paul G Higgins
Objectives: To investigate the mechanisms of tigecycline resistance in isogenic Acinetobacter baumannii isolate pairs as well as 65 unique clinical A. baumannii isolates obtained during the MagicBullet clinical trial from Greece, Italy and Spain. Methods: A. baumannii isolates were subjected to WGS and the regulatory genes of resistance-nodulation-cell division (RND)-type efflux pumps were analysed. MICs were determined by agar dilution and the expression of RND-type efflux pumps was measured by semi-quantitative RT-PCR...
March 14, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29548026/the-consistent-differential-expression-of-genetic-pathways-following-exposure-of-an-industrial-pseudomonas-aeruginosa-strain-to-preservatives-and-a-laundry-detergent-formulation
#9
Angharad E Green, Alejandro Amézquita, Yvan Le-Marc, Matthew J Bull, Thomas R Connor, Eshwar Mahenthiralingam
Pseudomonas aeruginosa (P. aeruginosa) is a common contaminant associated with product recalls in the home and personal care (HPC) industry. Preservation systems are used to prevent spoilage and protect consumers, but greater knowledge is needed of preservative resistance mechanisms used by P. aeruginosa contaminants. We aimed to identify genetic pathways associated with preservative exposure by using an industrial P. aeruginosa strain and implementing RNA-Seq to understand gene expression changes in response to industry relevant conditions...
March 14, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29506266/a-putative-rnd-type-efflux-pump-h239_3064-contributes-to-colistin-resistance-through-crrb-in-klebsiella-pneumoniae
#10
Yi-Hsiang Cheng, Tzu-Lung Lin, Yi-Tsung Lin, Jin-Town Wang
Background: Colistin is one of the last-resort antibiotics used to treat carbapenem-resistant Klebsiella pneumoniae infection. Our previous studies indicated that clinical strains encoding CrrB with amino acid substitutions exhibited higher colistin resistance (MICs ≥512 mg/L) than did colistin-resistant strains encoding mutant MgrB, PmrB or PhoQ. Objectives: CrrAB may regulate another unknown mechanism(s) contributing to colistin resistance, besides modifications of LPS with 4-amino-4-deoxy-l-arabinose and phosphoethanolamine...
February 28, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29424299/medicinal-chemistry-updates-on-bacterial-efflux-pump-modulators
#11
Fernando Andre Pereira Marques Duraes, Madalena Maria Magalhaes Pinto, Maria Emilia Silva Pereira de Sousa
Antibiotic resistance is one of the most pressing health issues of our days. It can arise due to a multiplicity of factors, such as target modification, decrease in the drug uptake, changes in the metabolic pathways and activation of efflux pumps. The overexpression of efflux pumps is responsible for the extrusion of drugs, making antibiotic therapy fail, as the quantity of intracellular antibiotic is not enough to provide the desired therapeutic effect. Efflux pumps can be included in five families according to their composition, nature of substrates, energy source, and number of transmembrane spanning regions...
February 9, 2018: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/29407044/computer-simulations-of-the-activity-of-rnd-efflux-pumps
#12
Attilio Vittorio Vargiu, Venkata Krishnan Ramaswamy, Giuliano Malloci, Ivana Malvacio, Alessio Atzori, Paolo Ruggerone
The putative mechanism by which bacterial RND-type multidrug efflux pumps recognize and transport their substrates is a complex and fascinating enigma of structural biology. How a single protein can recognize a huge number of unrelated compounds and transport them through one or just a few mechanisms is an amazing feature not yet completely unveiled. The appearance of cooperativity further complicates the understanding of structure-dynamics-activity relationships in these complex machineries. Experimental techniques may have limited access to the molecular determinants and to the energetics of key processes regulating the activity of these pumps...
January 31, 2018: Research in Microbiology
https://www.readbyqxmd.com/read/29394337/raising-the-stakes-loss-of-efflux-pump-regulation-decreases-meropenem-susceptibility-in-burkholderia-pseudomallei
#13
Derek S Sarovich, Jessica R Webb, Matthew C Pitman, Linda T Viberg, Mark Mayo, Robert W Baird, Jennifer M Robson, Bart J Currie, Erin P Price
Background: Burkholderia pseudomallei, the causative agent of the high-mortality disease melioidosis, is a Gram-negative bacterium that is naturally resistant to many antibiotics. There is no vaccine for melioidosis, and effective eradication is reliant on biphasic and prolonged antibiotic administration. The carbapenem drug, meropenem, is the current gold-standard option for treating severe melioidosis. Intrinsic B. pseudomallei resistance towards meropenem has not yet been documented; however, resistance could conceivably develop over the course of infection, leading to prolonged sepsis and treatment failure...
January 31, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29335824/broad-spectrum-resistance-of-pseudomonas-aeruginosa-from-shellfish-infrequent-acquisition-of-novel-resistance-mechanisms
#14
Ana Maravić, Ivica Šamanić, Matilda Šprung, Željana Fredotović, Nada Ilić, Josipa Dragičević, Jasna Puizina
Pseudomonas aeruginosa is one the most common multidrug-resistant pathogens worldwide. It has been previously detected in marine shellfish, but its antibiotic resistance in such environment has not been explored. By combining PCR detection of acquired genes, and resistance-nodulation-cell division (RND) efflux studying, we investigated the multifactorial resistance traits of 108 P. aeruginosa isolates recovered from wild-growing Mediterranean mussels (Mytilus galloprovincialis) in Croatia. Eleven different resistance profiles were found, with the main mechanism being the overexpression of intrinsic efflux pump(s), particularly MexAB-OprM...
January 15, 2018: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/29276505/contribution-of-raeb-a-putative-rnd-type-transporter-to-aminoglycoside-and-detergent-resistance-in-riemerella-anatipestifer
#15
Xin Zhang, Ming-Shu Wang, Ma-Feng Liu, De-Kang Zhu, Francis Biville, Ren-Yong Jia, Shun Chen, Kun-Feng Sun, Qiao Yang, Ying Wu, Xin-Xin Zhao, Xiao-Yue Chen, An-Chun Cheng
Riemerella anatipestifer is an important pathogenic bacterium that infects ducks. It exhibits resistance to multiple classes of antibiotics. Multidrug efflux pumps play a major role as a mechanism of antimicrobial resistance in Gram-negative pathogens and they are poorly understood in R. anatipestifer . In this study, a gene encoding the B739_0873 protein in R. anatipestifer CH-1, which belongs to the resistance-nodulation-cell division (RND) efflux pump family, was identified. With respect to the substrate specificity of B739_0873, the antibiotic susceptibility testing showed that the B739_0873 knockout strain was more sensitive to aminoglycosides and detergents than the wild-type strain...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29246087/in-silico-interaction-studies-suggest-rnd-efflux-pump-mediates-polymyxin-resistance-in-acinetobacter-baumannii
#16
Privita Verma, Pramila Maurya, Monalisa Tiwari, Vishvanath Tiwari
Bacterial efflux pumps have emerged as antibiotic resistance determinants and confers multi-drug resistance to a broad range of antimicrobials as well as non-antibiotic substances. A study about translocation of antibiotic molecules through the efflux transporter, will contribute in determining substrate specificity. In the present study, we have explored RND family efflux pump extensively found in Acinetobacter baumannii i.e. AdeABC. Besides, another well studied RND efflux pump, AcrAB-TolC together with a non-RND efflux pump, NorM was investigated for comparative analysis...
December 29, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/29235508/genetic-adaptation-of-microbial-populations-present-in-high-intensity-catfish-production-systems-with-therapeutic-oxytetracycline-treatment
#17
Qifan Zeng, Xiangli Tian, Luxin Wang
Microbial communities that are present in aquaculture production systems play significant roles in degrading organic matter, controlling diseases, and formation of antibiotic resistance. It is important to understand the diversity and abundance of microbial communities and their genetic adaptations associated with environmental physical and chemical changes. Here we collected water and sediment samples from a high-intensity catfish production system and its original water reservoir. The metagenomic analysis showed that Proteobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, and Firmicutes were the top five phyla identified from all samples...
December 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29217371/reconstitution-of-the-activity-of-rnd-efflux-pumps-a-bottom-up-approach
#18
Dhenesh Puvanendran, Quentin Cece, Martin Picard
Efflux pumps are systems devoted to the extrusion of noxious compounds. In this review, we discuss the various strategies that have thus far been undertaken for the investigation of efflux pumps after reconstitution into liposomes. It is challenging to uncover mechanisms and dynamics of efflux pumps due to a number of characteristics: their function depends on the correct assembly of three components and they span two adjacent membranes whose lipid compositions are very different. In addition, efflux pumps are active transporters that need energy to work...
December 5, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/29208490/fluorescence-enlightens-rnd-pump-activity-and-the-intrabacterial-concentration-of-antibiotics
#19
Muriel Masi, Estelle Dumont, Julia Vergalli, Jelena Pajovic, Matthieu Réfrégiers, Jean-Marie Pagès
To understand antibiotic resistance in Gram-negative bacteria, a key point is to investigate antibiotic accumulation, which is defined by influx and efflux. Several methods exist to evaluate membrane permeability and efflux pump activity, but they present disadvantages and limitations. An optimized spectrofluorimetric method using intrinsic tryptophan fluorescence as an internal standard, as well as a complementary microfluorimetric assay following time-course accumulation in intact individual cells, have been developed...
December 5, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/29203487/role-of-smeu1vwu2x-operon-in-alleviation-of-oxidative-stresses-and-occurrence-of-sulfamethoxazole-trimethoprim-resistant-mutants-in-stenotrophomonas-maltophilia
#20
Chao-Jung Wu, Tsu-Ting Chiu, Yi-Tsung Lin, Yi-Wei Huang, Li-Hua Li, Tsuey-Ching Yang
Overexpression of resistance-nodulation-division (RND)-type efflux pumps is an important mechanism for bacteria to combat antimicrobials. RND efflux pumps are also critical for bacterial physiology, such as oxidative stress tolerance. Stenotrophomonas maltophilia , a multidrug-resistant opportunistic pathogen, harbors eight RND-type efflux pump operons. Of these, the smeU1VWU2X operon is unique for its possession of two additional genes, smeU1 and smeU2 , which encode proteins of the short-chain dehydrogenase/reductase (SDR) family...
February 2018: Antimicrobial Agents and Chemotherapy
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