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RND efflux pump

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https://www.readbyqxmd.com/read/28352264/indole-induced-activities-of-%C3%AE-lactamase-and-efflux-pump-confer-ampicillin-resistance-in-pseudomonas-putida-kt2440
#1
Jisun Kim, Bora Shin, Chulwoo Park, Woojun Park
Indole, which is widespread in microbial communities, has received attention because of its effects on bacterial physiology. Pseudomonas putida and Pseudomonas aeruginosa can acquire ampicillin (Amp) resistance during growth on indole-Amp agar. Transcriptome, mutant, and inhibitor studies have suggested that Amp resistance induced by indole can be attributed to increased gene expression of ttgAB encoding two genes of RND-type multidrug efflux operons and an ampC encoding β-lactamase. Expression, enzyme activities, and mutational analyses indicated that AmpC β-lactamase is important for acquiring Amp resistance of P...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28314727/transcriptomic-analyses-elucidate-adaptive-differences-of-closely-related-strains-of-p-aeruginosa-in-fuel
#2
Thusitha S Gunasekera, Loryn L Bowen, Carol E Zhou, Susan C Howard-Byerly, William S Foley, Richard C Striebich, Larry C Dugan, Oscar N Ruiz
Pseudomonas aeruginosa can utilize hydrocarbons, but different strains have varying degrees of adaptation despite their highly conserved genome. P. aeruginosa ATCC 33988 is highly adapted to hydrocarbons while strain PAO1, a human pathogen, is less-adapted and degrades jet fuel at a slower rate than does ATCC 33988. We investigated fuel specific transcriptomic differences between these strains in order to ascertain the underling mechanisms utilized by the adapted strain to proliferate in fuel. During growth in fuel, the genes related to alkane degradation, heat-shock response, membrane proteins, efflux pumps and several novel genes were upregulated in ATCC 33988...
March 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28275586/colistin-resistance-in-acinetobacter-baumannii-mdr-zj06-revealed-by-a-multiomics-approach
#3
Xiaoting Hua, Lilin Liu, Youhong Fang, Qiucheng Shi, Xi Li, Qiong Chen, Keren Shi, Yan Jiang, Hua Zhou, Yunsong Yu
Acinetobacter baumannii has emerged as an important opportunistic pathogen due to its ability to acquire resistance to most currently available antibiotics. Colistin is often considered as the last line of therapy for infections caused by multidrug-resistant A. baumannii (MDRAB). However, colistin-resistant A. baumannii strain has recently been reported. To explore how multiple drug-resistant A. baumannii responded to colistin resistance, we compared the genomic, transcriptional and proteomic profile of A. baumannii MDR-ZJ06 to the induced colistin-resistant strain ZJ06-200P5-1...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28230771/antibiotic-resistance-determinant-focused-acinetobacter-baumannii-vaccine-designed-using-reverse-vaccinology
#4
Zhaohui Ni, Yan Chen, Edison Ong, Yongqun He
As one of the most influential and troublesome human pathogens, Acinetobacter baumannii (A. baumannii) has emerged with many multidrug-resistant strains. After collecting 33 complete A. baumannii genomes and 84 representative antibiotic resistance determinants, we used the Vaxign reverse vaccinology approach to predict classical type vaccine candidates against A. baumannii infections and new type vaccine candidates against antibiotic resistance. Our genome analysis identified 35 outer membrane or extracellular adhesins that are conserved among all 33 genomes, have no human protein homology, and have less than 2 transmembrane helices...
February 21, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28210464/evaluation-of-efflux-pumps-gene-expression-in-resistant-pseudomonas-aeruginosa-isolates-in-an-iranian-referral-hospital
#5
Babak Pourakbari, Sahar Yaslianifard, Somaye Yaslianifard, Shima Mahmoudi, Sepideh Keshavarz-Valian, Setareh Mamishi
BACKGROUND AND OBJECTIVES: Pseudomonas aeruginosa (PA) is one of the most important causes of nosocomial infections and has an intrinsic resistance to many antibiotics. Among all the resistance-nodulation-division (RND) pumps of P. aeruginosa, MexAB-OprM is the first efflux pump found to target multiple classes of antibiotics. This study was aimed to evaluate the expression level of genes expressing MexAB-OprM in clinical isolates of P. aeruginosa. MATERIALS AND METHODS: In this study, 45 P...
August 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28044273/rnd-efflux-pump-mediated-antibiotic-resistance-in-gram-negative-bacteria-escherichia-coli-and-pseudomonas-aeruginosa-a-major-issue-worldwide
#6
REVIEW
Minakshi Puzari, Pankaj Chetia
Therapeutic failures against diseases due to resistant Gram-negative bacteria have become a major threat nowadays as confirmed by surveillance reports across the world. One of the methods of development of multidrug resistance in Escherichia coli and Pseudomonas aeruginosa is by means of RND efflux pumps. Inhibition of these pumps might help to combat the antibiotic resistance problem, for which the structure and regulation of the pumps have to be known. Moreover, judicious antibiotic use is needed to control the situation...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28034459/calcium-induces-tobramycin-resistance-in-pseudomonas-aeruginosa-by-regulating-rnd-efflux-pumps
#7
Sharmily Khanam, Manita Guragain, Dirk L Lenaburg, Ryan Kubat, Marianna A Patrauchan
Pseudomonas aeruginosa is an opportunistic multidrug resistant pathogen causing severe chronic infections. Our previous studies showed that elevated calcium (Ca(2+)) enhances production of several virulence factors and plant infectivity of the pathogen. Here we show that Ca(2+) increases resistance of P. aeruginosa PAO1 to tobramycin, antibiotic commonly used to treat Pseudomonas infections. LC-MS/MS-based comparative analysis of the membrane proteomes of P aeruginosa grown at elevated versus not added Ca(2+), determined that the abundances of two RND (resistance-nodulation-cell division) efflux pumps, MexAB-OprM and MexVW-OprM, were increased in the presence of elevated Ca(2+)...
January 2017: Cell Calcium
https://www.readbyqxmd.com/read/27902383/detection-of-isaba1-in-association-with-a-novel-allelic-variant-of-the-%C3%AE-lactamase-adc-82-and-class-d-%C3%AE-lactamase-genes-mediating-carbapenem-resistance-among-the-clinical-isolates-of-mdr-a-baumannii
#8
Rajagopalan Saranathan, Rinki Kumari, Ramakrishnan Kalaivani, Sah Suresh, Anshu Rani, Shashikala Purty, Prashanth K
The objective of the present study is to investigate the diverse resistance determinants, their association with IS mobile elements and predilection of a particular clone for such associations in A. baumannii. Fifty-four consecutive isolates collected during 2011-2012 from a tertiary care hospital were subjected to susceptibility testing followed by PCR screening of commonly reported β-lactamases and 16S rRNA methyl transferase encoding genes. The integrity of RND efflux pump related genes in their respective operons was also investigated...
November 25, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27879336/beyond-antimicrobial-resistance-evidence-for-a-distinct-role-of-the-acrd-efflux-pump-in-salmonella-biology
#9
Michelle M C Buckner, Jessica M A Blair, Roberto M La Ragione, Jane Newcombe, Daniel J Dwyer, Alasdair Ivens, Laura J V Piddock
For over 20 years, bacterial multidrug resistance (MDR) efflux pumps have been studied because of their impact on resistance to antimicrobials. However, critical questions remain, including why produce efflux pumps under non-antimicrobial treatment conditions, and why have multiple pumps if their only purpose is antimicrobial efflux? Salmonella spp. possess five efflux pump families, including the resistance-nodulation-division (RND) efflux pumps. Notably, the RND efflux pump AcrD has a unique substrate profile, distinct from other Salmonella efflux pumps...
November 22, 2016: MBio
https://www.readbyqxmd.com/read/27825482/unraveling-genomic-and-phenotypic-nature-of-multidrug-resistant-mdr-pseudomonas-aeruginosa-vrfpa04-isolated-from-keratitis-patient
#10
Murugan N, Malathi J, Umashankar V, Madhavan H N
Multidrug-resistant (MDR) Pseudomonas aeruginosa VRFPA04, obtained from a keratitis patient was found to exhibit resistance to betalactam (Penicillins, cephalosporins, including carbapenems, except aztreonam), aminoglycosides, quinolone group of drugs and susceptible to colistin. The complete genome sequencing of the ocular isolate to measure and ascertain the degree of multidrug resistance in VRFPA04 strain resulted in 6,818,030bp (6.8Mb) genome sizes, which happen to be the third largest genome available in the Genbank to date...
December 2016: Microbiological Research
https://www.readbyqxmd.com/read/27815276/genome-dynamics-and-molecular-infection-epidemiology-of-multidrug-resistant-helicobacter-pullorum-isolates-obtained-from-broiler-and-free-range-chickens-in-india
#11
Shamsul Qumar, Mohammad Majid, Narender Kumar, Sumeet K Tiwari, Torsten Semmler, Savita Devi, Ramani Baddam, Arif Hussain, Sabiha Shaik, Niyaz Ahmed
Some life-threatening, foodborne, and zoonotic infections are transmitted through poultry birds. Inappropriate and indiscriminate use of antimicrobials in the livestock industry has led to an increased prevalence of multidrug-resistant bacteria with epidemic potential. Here, we present a functional molecular epidemiological analysis entailing the phenotypic and whole-genome sequence-based characterization of 11 H. pullorum isolates from broiler and free-range chickens sampled from retail wet markets in Hyderabad City, India...
January 1, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27806930/rnd-efflux-pump-and-its-interrelationship-with-quorum-sensing-system
#12
Liang Zhibin, Chen Yumei, Chen Yufan, Cheng Yingying, Zhang Lianhui
Antibiotic resistance has become a serious concern in treatment of bacterial infections. Overexpression of efflux pump is one of the important mechanisms in antibiotic resistance. In Gram negative bacteria, RND (Resistance-nodulation-cell division) superfamily efflux pump plays a vital important role in antibiotics resistance. Recent research progress unveils an intriguing interrelationship between RND efflux pump and the bacterial quorum sensing system, whose regulation is dependent on small signal molecules...
October 20, 2016: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/27789287/effect-of-site-directed-mutations-in-multidrug-efflux-pump-acrb-examined-by-quantitative-efflux-assays
#13
Alfred D Kinana, Attilio V Vargiu, Hiroshi Nikaido
BACKGROUND: The Resistance-Nodulation-Division (RND) family transporter AcrB plays a major role in the intrinsic and increased resistance of Escherichia coli to a large number of antibiotics. The distal binding pocket within this multidrug efflux transporter is very large, but the effort to define the roles of various residues facing this pocket through site-directed mutagenesis so far involved only the determination of minimal inhibitory concentrations of drugs in mutants. METHODS: We measured in intact E...
November 25, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27764576/multidrug-efflux-pumps-and-their-role-in-antibiotic-and-antiseptic-resistance-a-pharmacodynamic-perspective
#14
REVIEW
Sandrine Alibert, Joannah N'gompaza Diarra, Jessica Hernandez, Aurélien Stutzmann, Marwa Fouad, Gérard Boyer, Jean-Marie Pagès
Worrying levels of bacterial resistance have been reported worldwide involving the failure of many available antibiotic treatments. Multidrug resistance (MDR) in Gram-negative bacteria is often ascribed to the presence of multiple and different resistance mechanisms in the same strain. RND efflux pumps play a major role and are an attractive target to discover new antibacterial drugs. Areas covered: This review discusses the prevalence of efflux pumps, their overexpression in clinical scenarios, their polyselectivity, their effect on the intracellular concentrations of various antibiotics associated with the alteration of the membrane permeability and their involvement in pathogenicity are discussed...
March 2017: Expert Opinion on Drug Metabolism & Toxicology
https://www.readbyqxmd.com/read/27736975/cpxr-activates-mexab-oprm-efflux-pump-expression-and-enhances-antibiotic-resistance-in-both-laboratory-and-clinical-nalb-type-isolates-of-pseudomonas-aeruginosa
#15
Zhe-Xian Tian, Xue-Xian Yi, Anna Cho, Fergal O'Gara, Yi-Ping Wang
Resistance-Nodulation-Division (RND) efflux pumps are responsible for multidrug resistance in Pseudomonas aeruginosa. In this study, we demonstrate that CpxR, previously identified as a regulator of the cell envelope stress response in Escherichia coli, is directly involved in activation of expression of RND efflux pump MexAB-OprM in P. aeruginosa. A conserved CpxR binding site was identified upstream of the mexA promoter in all genome-sequenced P. aeruginosa strains. CpxR is required to enhance mexAB-oprM expression and drug resistance, in the absence of repressor MexR, in P...
October 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27645384/opening-the-channel-the-two-functional-interfaces-of-pseudomonas-aeruginosa-opmh-with-the-triclosan-efflux-pump-triabc
#16
Abigail T Ntreh, Jon W Weeks, Logan M Nickels, Helen I Zgurskaya
TriABC-OpmH is an efflux pump from Pseudomonas aeruginosa with an unusual substrate specificity and protein composition. When overexpressed, this pump confers a high level of resistance to the biocide triclosan and the detergent SDS, which are commonly used in combinations for antimicrobial treatments. This activity requires an RND transporter (TriC), an outer membrane channel (OpmH), and two periplasmic membrane fusion proteins (TriA and TriB) with nonequivalent functions. In the active complex, TriA is responsible for the recruitment of OpmH, while TriB is responsible for stimulation of the transporter TriC...
December 1, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27450181/screening-of-natural-products-and-derivatives-for-the-identification-of-rnd-efflux-pump-inhibitors
#17
Debarati Choudhury, Anupam Das Talukdar, Pankaj Chetia, Amitabha Bhattacharjee, Manabendra Dutta Choudhury
AIM AND OBJECTIVE: Overexpression of efflux pumps belonging to the Resistance Nodulation cell Division (RND) family is the most important intrinsic resistance mechanism of Pseudomonas aeruginosa. Hence, it is imperative to identify suitable efflux pump inhibitors (EPI) that can lead to increased intracellular concentration of antibiotics by blocking the pump. This study was undertaken to identify a putative plant based efflux pump inhibitor for RND efflux pump of P. aeruginosa. MATERIAL AND METHOD: Using molecular docking approach, 328 secondary plant metabolites have been screened for their inhibitory activity against cytoplasmic exporter protein MexB of MexAB-OprM efflux pump of P...
2016: Combinatorial Chemistry & High Throughput Screening
https://www.readbyqxmd.com/read/27332787/distribution-and-expression-of-the-ade-multidrug-efflux-systems-in-acinetobacter-baumannii-clinical-isolates
#18
Sirawit Pagdepanichkit, Chanwit Tribuddharat, Rungtip Chuanchuen
One hundred Acinetobacter baumannii clinical isolates were examined for inhibitory effect of reserpine and carbonyl cyanide m-chlorophenylhydrazone (CCCP) on the antimicrobial susceptibility and expression of 4 resistant-nodulation-cell division (RND)-type multidrug efflux systems, including AdeABC, AdeDE, AdeIJK, and AdeFGH, using RT-PCR. Ten A. baumannii isolates expressing AdeABC, AdeIJK, or AdeFGH were randomly selected for determination of transcription level and regulatory mutations. While all the isolates were resistant to multiple drugs, the reserpine and CCCP experiment showed that the multidrug resistance phenotype in most A...
September 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27256586/current-perspectives-on-tigecycline-resistance-in-enterobacteriaceae-susceptibility-testing-issues-and-mechanisms-of-resistance
#19
REVIEW
Spyros Pournaras, Vasiliki Koumaki, Nicholas Spanakis, Vasiliki Gennimata, Athanassios Tsakris
During the past decades, rates of multidrug-resistant (MDR) and carbapenem-resistant (CR) Enterobacteriaceae clinical isolates, mainly Klebsiella spp., Escherichia coli, Enterobacter spp., Proteus spp. and Serratia marcescens, have increased, considerably restricting effective antimicrobial treatments. Tigecycline, the first member of the glycylcyclines, has been approved by the US Food and Drug Administration (FDA) for the treatment of complicated skin and soft-tissue, complicated intra-abdominal and community-acquired bacterial respiratory infections and is increasingly administered against MDR Enterobacteriaceae...
July 2016: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/27247231/contribution-of-the-ade-resistance-nodulation-cell-division-type-efflux-pumps-to-fitness-and-pathogenesis-of-acinetobacter-baumannii
#20
Eun-Jeong Yoon, Viviane Balloy, Laurence Fiette, Michel Chignard, Patrice Courvalin, Catherine Grillot-Courvalin
UNLABELLED: Overexpression of chromosomal resistance-nodulation-cell division (RND)-type efflux systems with broad substrate specificity contributes to multidrug resistance (MDR) in Acinetobacter baumannii We have shown that modulation of expression of the structural genes for the efflux systems AdeABC and AdeIJK confers MDR and results in numerous alterations of membrane-associated cellular functions, in particular biofilm formation. However, the contribution of these RND pumps to cell fitness and virulence has not yet been studied...
2016: MBio
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