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https://www.readbyqxmd.com/read/29720052/high-density-lipoprotein-mean-platelet-volume-and-uric-acid-as-biomarkers-for-outcomes-in-patients-with-sepsis-an-observational-study
#1
Luis Bolívar Montero-Chacón, Juan Ignacio Padilla-Cuadra, Sy Han Chiou, Gabriel Torrealba-Acosta
BACKGROUND: We conducted an observational study evaluating the association between uric acid, mean platelet volume (MPV), and high-density lipoprotein (HDL) with complications and outcomes of patients with sepsis in a critical care setting. METHODS: We followed patients with a diagnosis of severe sepsis and septic shock for a maximum of 28 days. Main outcomes assessed included length of stay (LOS), the need for renal replacement therapy (RRT), assisted mechanical ventilation (AMV), and vasopressor support as well as in-unit mortality...
January 1, 2018: Journal of Intensive Care Medicine
https://www.readbyqxmd.com/read/29653055/solid-phase-nasba-and-length-scale-effects-during-rna-amplification
#2
Youlong Ma, Feiyue Teng, Matthew R Libera
Solid-phase oligonucleotide amplification is of interest because of possible applications to next-generation sequencing, multiplexed microarray-based detection, and cell-free synthetic biology. Its efficiency is, however, less than that of traditional liquid-phase amplification involving unconstrained primers and enzymes, and understanding how to optimize the solid-phase amplification process remains challenging. Here we demonstrate the concept of solid-phase nucleic acid sequence based amplification (SP-NASBA) and use it to study the effect of tethering density on amplification efficiency...
April 13, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29330371/transcriptional-inaccuracy-threshold-attenuates-differences-in-rna-dependent-dna-synthesis-fidelity-between-retroviral-reverse-transcriptases
#3
Alba Sebastián-Martín, Verónica Barrioluengo, Luis Menéndez-Arias
In M13mp2 lacZα forward mutation assays measuring intrinsic fidelity of DNA-dependent DNA synthesis, wild-type human immunodeficiency virus type 1 (HIV-1) RTs of group M/subtype B previously showed >10-fold higher error rates than murine leukaemia virus (MLV) and avian myeloblastosis virus (AMV) RTs. An adapted version of the assay was used to obtain error rates of RNA-dependent DNA synthesis for several RTs, including wild-type HIV-1BH10 , HIV-1ESP49 , AMV and MLV RTs, and the high-fidelity mutants of HIV-1ESP49 RT K65R and K65R/V75I...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29182696/the-effect-of-the-functional-ionic-group-of-the-viologen-derivative-on-visible-light-driven-co-2-reduction-to-formic-acid-with-the-system-consisting-of-water-soluble-zinc-porphyrin-and-formate-dehydrogenase
#4
S Ikeyama, Y Amao
The effect of the functional ionic group of 4,4'-bipyridinium salt derivatives (4,4'-BPs) as the electron carrier on the visible-light driven conversion of CO2 to formic acid with the system consisting of water-soluble zinc tetraphenylporphyrin tetrasulfonate (ZnTPPS) and formate dehydrogenase (FDH) in the presence of triethanolamine (TEOA) as an electron donor was investigated. 1,1'-Diaminoethyl- (DAV), 1-aminoethyl-1'-methyl- (AMV), 1-carboxymethyl-1'-methyl- (CMV) and 1,1'-dicarboxymethyl-4,4'-bipyridinium salt (DCV) were prepared as the 4,4'-BPs with the functional ionic group...
January 17, 2018: Photochemical & Photobiological Sciences
https://www.readbyqxmd.com/read/29169202/accessory-mitral-valve-mimicking-aortic-valve-endocarditis-as-a-cause-of-cerebrovascular-accident
#5
Benjamin C Degner, Mario Gaudino, Erin Iannacone, Christopher Lau, Leonard N Girardi
Accessory mitral valve (AMV) is a rare congenital cardiac malformation associated with left ventricular outflow tract obstruction and endocarditis. We report a patient with an AMV who presented with a cerebellar infarct and discuss the management of this anomaly.
November 2017: Journal of Cardiac Surgery
https://www.readbyqxmd.com/read/29103920/influence-of-the-anteromedial-thalamus-on-social-defeat-associated-contextual-fear-memory
#6
Miguel J Rangel, Marcus Vinicius C Baldo, Newton Sabino Canteras
The ventral part of the anteromedial thalamic nucleus (AMv) is heavily targeted by the dorsal premammillary nucleus (PMd), which is the main hypothalamic site that is responsive to both predator and conspecific aggressor threats. This PMd-AMv pathway is likely involved in modulating memory processing, and previous findings from our group have shown that cytotoxic lesions or pharmacological inactivation of the AMv drastically reduced contextual fear responses to predator-associated environments. In the present study, we investigated the role of the AMv in both unconditioned (i...
February 26, 2018: Behavioural Brain Research
https://www.readbyqxmd.com/read/28947875/incidence-of-pests-and-viral-disease-on-pepino-solanum-muricatum-ait-in-kanagawa-prefecture-japan
#7
Ok-Kyung Kim, Tadashi Ishikawa, Yoshihiro Yamada, Takuma Sato, Hirosuke Shinohara, Ken Takahata
BACKGROUND: The solanaceous fruit crop pepino (Solanum muricatum Ait.), originating in the Andes, is grown commercially in South American countries and New Zealand. In these areas, pests and diseases of pepino have been identified well; however, to date, these have seldom been investigated in detail in Japan. Herein, we attempt to reconstruct an agricultural production system for commercial pepino crops in Japan, and evaluate the incidence of pests and viral diseases on pepino. The findings of this study will facilitate in developing a better crop system for the commercial cultivation of healthy pepino fruits...
2017: Biodiversity Data Journal
https://www.readbyqxmd.com/read/28923956/-arabidopsis-m-6-a-demethylase-activity-modulates-viral-infection-of-a-plant-virus-and-the-m-6-a-abundance-in-its-genomic-rnas
#8
Mireya Martínez-Pérez, Frederic Aparicio, Maria Pilar López-Gresa, Jose María Bellés, Jesus A Sánchez-Navarro, Vicente Pallás
N6 -methyladenosine (m6 A) is an internal, reversible nucleotide modification that constitutes an important regulatory mechanism in RNA biology. Unlike mammals and yeast, no component of the m6 A cellular machinery has been described in plants at present. m6 A has been identified in the genomic RNAs of diverse mammalian viruses and, additionally, viral infection was found to be modulated by the abundance of m6 A in viral RNAs. Here we show that the Arabidopsis thaliana protein atALKBH9B (At2g17970) is a demethylase that removes m6 A from single-stranded RNA molecules in vitro...
October 3, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28887190/rapid-detection-of-fifteen-known-soybean-viruses-by-dot-immunobinding-assay
#9
Akhtar Ali
A dot-immunobinding assay (DIBA) was optimized and used successfully for the rapid detection of 15 known viruses [Alfalfa mosaic virus (AMV), Bean pod mottle virus (BPMV), Bean yellow mosaic virus (BYMV), Cowpea mild mottle virus (CPMMV), Cowpea severe mosaic virus (CPSMV), Cucumber mosaic virus (CMV), Peanut mottle virus (PeMoV), Peanut stunt virus (PSV), Southern bean mosaic virus (SBMV), Soybean dwarf virus (SbDV), Soybean mosaic virus (SMV), Soybean vein necrosis virus (SVNV), Tobacco ringspot virus (TRSV), Tomato ringspot virus (ToRSV), and Tobacco streak virus (TSV)] infecting soybean plants in Oklahoma...
September 6, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28855829/detection-of-new-viruses-in-alfalfa-weeds-and-cultivated-plants-growing-adjacent-to-alfalfa-fields-in-saudi-arabia
#10
I M Al-Shahwan, O A Abdalla, M A Al-Saleh, M A Amer
A total of 1368 symptomatic plant samples showing different virus-like symptoms such as mottling, chlorosis, mosaic, yellow mosaic, vein clearing and stunting were collected from alfalfa, weed and cultivated plant species growing in vicinity of alfalfa fields in five principal regions of alfalfa production in Saudi Arabia. DAS-ELISA test indicated occurrence of 11 different viruses in these samples, 10 of which were detected for the first time in Saudi Arabia. Eighty percent of the alfalfa samples and 97.5% of the weed and cultivated plants samples were found to be infected with one or more of these viruses...
September 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28700020/a-meta-analysis-of-circulating-microvesicles-in-patients-with-myocardial-infarction
#11
Zhida Wang, Wang Cai, Shaolan Hu, Yufei Xia, Yao Wang, Qi Zhang, Liming Chen
Background: Cell-derived microvesicles (MVs) are vesicles released from activated or apoptotic cells. However, the levels of MVs in myocardial infarction have been found inconsistent in researches. Objective: To assess the association between MVs and myocardial infarction by conducting a meta-analysis. Methods: A systematic literature search on PubMed, Embase, Cochran, Google Scholar electronic database was conducted. Comparison of the MVs levels between myocardial infarction patients and healthy persons were included in our study...
July 10, 2017: Arquivos Brasileiros de Cardiologia
https://www.readbyqxmd.com/read/28694514/within-host-evolution-of-segments-ratio-for-the-tripartite-genome-of-alfalfa-mosaic-virus
#12
Beilei Wu, Mark P Zwart, Jesús A Sánchez-Navarro, Santiago F Elena
The existence of multipartite viruses is an intriguing mystery in evolutionary virology. Several hypotheses suggest benefits that should outweigh the costs of a reduced transmission efficiency and of segregation of coadapted genes associated with encapsidating each segment into a different particle. Advantages range from increasing genome size despite high mutation rates, faster replication, more efficient selection resulting from reassortment during mixed infections, better regulation of gene expression, or enhanced virion stability and cell-to-cell movement...
July 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28647416/related-factors-of-asymmetrical-vein-sign-in-acute-middle-cerebral-artery-stroke-and-correlation-with-clinical-outcome
#13
Jinchao Yu, Liming Wang, Zhenzhi Li, Shanshan Wang, Guangbin Wang
OBJECTIVE: The aim of our study was to analyze the related factors of asymmetrical cortical vein sign (ACVS) and asymmetrical medullary vein sign (AMVS) on susceptibility-weighted imaging (SWI) in patients with acute middle cerebral artery (MCA) stroke and whether their presence can be used as an independent predictor for clinical outcome. METHODS: According to the presence of ACVS and AMVS on SWI, 124 patients with acute MCA stroke within 3 days were divided into several different groups...
October 2017: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
https://www.readbyqxmd.com/read/28578114/inside-vietnam-s-largest-neurosurgery-department
#14
Constantine L Karras, Huy Minh Tran, David Dornbos, Phong Nguyen, Shahid M Nimjee, Daniel M Prevedello, Ciarán J Powers
Cho Ray Hospital is the premier tertiary care center in Ho Chi Minh City, Vietnam's most populous city. The neurosurgical department was founded in 1958 and is currently one of the busiest in the world. Last year the department was responsible for more than 10,000 neurosurgical operations, excluding neuroendovascular cases. Most of the elective cases are craniotomies for complex lesions such as aneurysms, arteriovenous malformations, skull base tumors, gliomas, and meningiomas. Although the neurosurgeons within the department are technically gifted, doing more with less predominates as the mantra at Cho Ray Hospital...
September 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28391536/plasma-homocysteine-levels-are-associated-with-macular-thickness-in-type-2-diabetes-without-diabetic-macular-edema
#15
Ning Dong, Hong Shi, Xin Tang
PURPOSE: To explore the relationships between macular thickness and the plasma concentrations of homocysteine, vitamin B12, folate, and other known risk factors for patients with diabetes without diabetic macular edema (DME). METHODS: Fasting venous blood samples were collected from 252 subjects (126 relatively healthy subjects with type 2 diabetes without diabetic macular edema and 126 age- and gender-matched controls). Measurement of macular thickness and volume was performed for those subjects using SD-OCT...
April 2018: International Ophthalmology
https://www.readbyqxmd.com/read/28387379/relationship-between-porcine-sperm-motility-and-sperm-enzymatic-activity-using-paper-based-devices
#16
Koji Matsuura, Han-Wei Huang, Ming-Cheng Chen, Yu Chen, Chao-Min Cheng
Mammalian sperm motility has traditionally been analyzed to determine fertility using computer-assisted semen analysis (CASA) systems. To develop low-cost and robust male fertility diagnostics, we created a paper-based MTT assay and used it to estimate motile sperm concentration. When porcine sperm motility was inhibited using sperm enzyme inhibitors for sperm enzymes related to mitochondrial activity and glycolysis, we simultaneously recorded sperm motility and enzymatic reactivity using a portable motility analysis system (iSperm) and a paper-based MTT assay, respectively...
April 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28357648/ameliorated-effects-of-epigallocatechin-gallate-against-toxicity-induced-by-vanadium-in-the-kidneys-of-wistar-rats
#17
Ahlem Soussi, Raed Abdennabi, Fatma Ghorbel, Jean-Claude Murat, Abdel Fettah El Feki
The aim of the study was to assess the protective effect of (-)-epigallocatechin gallate (EGCG), a flavonoid abundant in green tea, against ammonium metavanadate (AMV)-induced oxidative stress in male Wistar rats. Four groups of animals have been used, a control group and three test groups. In the first test group, AMV was intra-peritoneally (i.p) injected daily (5 mg/kg body weight for five consecutive days). The second test group of animals was also injected daily with EGCG (5 mg/kg body weight) during the same period...
December 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/27697453/the-functional-analysis-of-distinct-tospovirus-movement-proteins-ns-m-reveals-different-capabilities-in-tubule-formation-cell-to-cell-and-systemic-virus-movement-among-the-tospovirus-species
#18
Mikhail O Leastro, Vicente Pallás, Renato O Resende, Jesús A Sánchez-Navarro
The lack of infectious tospovirus clones to address reverse genetic experiments has compromised the functional analysis of viral proteins. In the present study we have performed a functional analysis of the movement proteins (NSM ) of four tospovirus species Bean necrotic mosaic virus (BeNMV), Chrysanthemum stem necrosis virus (CSNV), Tomato chlorotic spot virus (TCSV) and Tomato spotted wilt virus (TSWV), which differ biologically and molecularly, by using the Alfalfa mosaic virus (AMV) model system. All NSM proteins were competent to: i) support the cell-to-cell and systemic transport of AMV, ii) generate tubular structures on infected protoplast and iii) transport only virus particles...
January 2, 2017: Virus Research
https://www.readbyqxmd.com/read/27604673/inferior-vena-cava-resection-and-reconstruction-for-tumoral-recurrence-after-right-nephrectomy
#19
Pv-H Botianu, R Chirtes, C Marcu, H Kosza, M Stoian, O Brusnic, Amv Botianu, A Dobre
We report a 60 years old patient who was admitted for a local recurrence after a right nephrectomy performed 2 years ago (papillary renal carcinoma with areas of sarcomatoid differentiation - pT3a). CT scan showed a retroperitoneal mass with invasion of the inferior vena cava. We performed a complete en-bloque excision of the tumor with the infrarenal portion of the inferior vena cava and lympha-denectomy. The vascular reconstruction was performed by the interposition of a 20 mm diameter Dacron prosthesis. The postoperative course was complicated due to an episode of digestive bleeding (duodenal ulcer) which stopped after conservative treatment (antisecretory and hemostatics, including rFVIIa), but eventually favourable...
July 2016: Chirurgia
https://www.readbyqxmd.com/read/27494171/a-comparison-study-of-single-echo-susceptibility-weighted-imaging-and-combined-multi-echo-susceptibility-weighted-imaging-in-visualizing-asymmetric-medullary-veins-in-stroke-patients
#20
COMPARATIVE STUDY
Chao Wang, Tiantian Qiu, Ruirui Song, Yerfan Jiaerken, Linglin Yang, Shaoze Wang, Minming Zhang, Xinfeng Yu
BACKGROUND: Asymmetric medullary veins (AMV) are frequently observed in stroke patients and single-echo susceptibility weighted imaging (SWIs) is the main technique in detecting AMV. Our study aimed to investigate which echo time (TE) on single-echo susceptibility is the optimal echo for visualizing AMV and to compare the ability in detecting AMV in stroke patients between SWIs and multi-echo susceptibility weighted imaging (SWIc). MATERIALS AND METHODS: Twenty patients with middle cerebral artery stroke were included...
2016: PloS One
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