keyword
MENU ▼
Read by QxMD icon Read
search

Gardnerella biofilm

keyword
https://www.readbyqxmd.com/read/28922066/the-effect-of-lysozyme-on-reducing-biofilms-by-staphylococcus-aureus-pseudomonas-aeruginosa-and-gardnerella-vaginalis-an-in-vitro-examination
#1
Mirsada Hukić, Dzenita Seljmo, Amra Ramovic, Monia Avdić Ibrišimović, Serkan Dogan, Jasna Hukic, Elma Feric Bojic
Two basic questions about lysozyme activities on the selected microorganisms were investigated, namely whether lysozyme inhibits biofilm production and which concentrations of the enzyme have the ability to change the natural biofilm producing capacity of different strains of Staphylococcus aureus (methicillin sensitive and resistant), Streptococcus pyogenes, Pseudomonas aeruginosa, and Gardnerella vaginalis. The effect of lysozyme on biofilm formation capacities of 16 strains of selected microorganisms was investigated, whereby four testing replicates have been performed in vitro using the Test Tube method, and the potential of lysozyme to change biofilm forming capacities depending on its concentration, species, and strains of microorganisms is demonstrated...
September 18, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28903767/treatment-of-biofilms-in-bacterial-vaginosis-by-an-amphoteric-tenside-pessary-clinical-study-and-microbiota-analysis
#2
Cornelia Gottschick, Zhi-Luo Deng, Marius Vital, Clarissa Masur, Christoph Abels, Dietmar H Pieper, Manfred Rohde, Werner Mendling, Irene Wagner-Döbler
BACKGROUND: Bacterial vaginosis (BV) is the most common vaginal syndrome among women in their reproductive years. It is associated with an increased risk of acquiring sexually transmitted infections and complications like preterm labor. BV is characterized by a high recurrence rate for which biofilms frequently found on vaginal epithelial cells may be a reason. RESULTS: Here, we report a controlled randomized clinical trial that tested the safety and effectiveness of a newly developed pessary containing an amphoteric tenside (WO3191) to disrupt biofilms after metronidazole treatment of BV...
September 13, 2017: Microbiome
https://www.readbyqxmd.com/read/28893789/gemini-cationic-amphiphiles-control-biofilm-formation-by-bacterial-vaginosis-pathogens
#3
Ammar Algburi, Yingyue Zhang, Richard Weeks, Nicole Comito, Saskia Zehm, Juanita Pinto, Kathryn E Uhrich, Michael L Chikindas
Antibiotic resistance and recurrence of bacterial vaginosis (BV), a polymicrobial infection, justify the need for novel antimicrobials to counteract microbial resistance to conventional antibiotics. Previously, two series of cationic amphiphiles (CAms), which self-assemble into supramolecular nanostructures with membrane-lytic properties, were designed with hydrophilic head groups and non-polar domains. The combination of CAms with commonly prescribed antibiotics is suggested as a promising strategy for targeting microorganisms that are resistant to conventional antibiotics...
September 11, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28860468/characterization-of-cervico-vaginal-microbiota-in-women-developing-persistent-high-risk-human-papillomavirus-infection
#4
Monica Di Paola, Cristina Sani, Ann Maria Clemente, Anna Iossa, Eloisa Perissi, Giuseppe Castronovo, Michele Tanturli, Damariz Rivero, Federico Cozzolino, Duccio Cavalieri, Francesca Carozzi, Carlotta De Filippo, Maria Gabriella Torcia
Changes in cervico-vaginal microbiota with Lactobacillus depletion and increased microbial diversity facilitate human papillomavirus (HPV) infection and might be involved in viral persistence and cancer development. To define the microbial Community State Types (CSTs) associated with high-risk HPV-persistence, we analysed 55 cervico-vaginal samples from HPV positive (HPV+) women out of 1029 screened women and performed pyrosequencing of 16S rDNA. A total of 17 samples from age-matched HPV negative (HPV-) women were used as control...
August 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28807017/the-urinary-microbiota-of-men-and-women-and-its-changes-in-women-during-bacterial-vaginosis-and-antibiotic-treatment
#5
Cornelia Gottschick, Zhi-Luo Deng, Marius Vital, Clarissa Masur, Christoph Abels, Dietmar H Pieper, Irene Wagner-Döbler
BACKGROUND: The urinary microbiota is similarly complex as the vaginal and penile microbiota, yet its role as a reservoir for pathogens and for recurrent polymicrobial biofilm diseases like bacterial vaginosis (BV) is not clear. RESULTS: Here, we analysed the urinary microbiota of healthy men and women and compared it with that of women during BV and after antibiotic treatment using next-generation sequencing of the 16S rRNA gene V1-V2 regions. Eight different community types, so called urotypes (UT), were identified in healthy humans, all of which were shared between men and women, except UT 7, dominated in relative abundance by Lactobacillus crispatus, which was found in healthy women only...
August 14, 2017: Microbiome
https://www.readbyqxmd.com/read/28792372/treatment-failure-of-bacterial-vaginosis-is-not-associated-with-higher-loads-of-atopobium-vaginae-and-gardnerella-vaginalis
#6
Carolina Sanitá Tafner Ferreira, Gilbert Gerard Donders, Cristina Maria Garcia de Lima Parada, Andrea da Rocha Tristão, Thaiz Fernandes, Márcia Guimarães da Silva, Camila Marconi
PURPOSE: Cervicovaginal Atopobium vaginae and Gardnerella vaginalis are strongly associated with bacterial vaginosis (BV) and are the main components of vaginal biofilms. The low efficacy of BV treatment with metronidazole may be due to the presence of such biofilms. Thus, the aim of this study was to compare the pretreatment cervicovaginal loads of A. vaginae and G. vaginalis for women who restored normal flora and those who persisted with BV after a full course of oral metronidazole...
August 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28649404/comparative-transcriptomic-analysis-of-gardnerella-vaginalis-biofilms-vs-planktonic-cultures-using-rna-seq
#7
Joana Castro, Angela França, Katie R Bradwell, Myrna G Serrano, Kimberly K Jefferson, Nuno Cerca
Bacterial vaginosis is the most common gynecological disorder affecting women of reproductive age. Bacterial vaginosis is frequently associated with the development of a Gardnerella vaginalis biofilm. Recent data indicates that G. vaginalis biofilms are more tolerant to antibiotics and are able to incorporate other bacterial vaginosis -associated species, yielding a multi-species biofilm. However, despite its apparent role in bacterial vaginosis, little is known regarding the molecular determinants involved in biofilm formation by G...
2017: NPJ Biofilms and Microbiomes
https://www.readbyqxmd.com/read/28594946/association-of-vaginal-dysbiosis-and-biofilm-with-contraceptive-vaginal-ring-biomass-in-african-women
#8
Liselotte Hardy, Vicky Jespers, Irith De Baetselier, Jozefien Buyze, Lambert Mwambarangwe, Viateur Musengamana, Janneke van de Wijgert, Tania Crucitti
We investigated the presence, density and bacterial composition of contraceptive vaginal ring biomass and its association with the vaginal microbiome. Of 415 rings worn by 120 Rwandese women for three weeks, the biomass density was assessed with crystal violet and the bacterial composition of biomass eluates was assessed with quantitative polymerase chain reaction (qPCR). The biomass was visualised after fluorescence in situ hybridisation (FISH) and with scanning electron microscopy (SEM). The vaginal microbiome was assessed with Nugent scoring and vaginal biofilm was visualised after FISH...
2017: PloS One
https://www.readbyqxmd.com/read/28339292/thymbra-capitata-essential-oil-as-potential-therapeutic-agent-against-gardnerella-vaginalis-biofilm-related-infections
#9
Daniela Machado, Carlos Gaspar, Ana Palmeira-de-Oliveira, Carlos Cavaleiro, Lígia Salgueiro, José Martinez-de-Oliveira, Nuno Cerca
AIM: To evaluate the antibacterial activity of Thymbra capitata essential oil and its main compound, carvacrol, against Gardnerella vaginalis grown planktonically and as biofilms, and its effect of vaginal lactobacilli. MATERIALS & METHODS: Minimal inhibitory concentration, minimal lethal concentration determination and flow cytometry analysis were used to assess the antibacterial effect against planktonic cells. Antibiofilm activity was measured through quantification of biomass and visualization of biofilm structure by confocal laser scanning microscopy...
April 2017: Future Microbiology
https://www.readbyqxmd.com/read/28241058/the-presence-of-the-putative-gardnerella-vaginalis-sialidase-a-gene-in-vaginal-specimens-is-associated-with-bacterial-vaginosis-biofilm
#10
Liselotte Hardy, Vicky Jespers, Magelien Van den Bulck, Jozefien Buyze, Lambert Mwambarangwe, Viateur Musengamana, Mario Vaneechoutte, Tania Crucitti
Bacterial vaginosis (BV) is a difficult-to-treat recurrent condition in which health-associated lactobacilli are outnumbered by other anaerobic bacteria, such as Gardnerella vaginalis. Certain genotypes of G. vaginalis can produce sialidase, while others cannot. Sialidase is known to facilitate the destruction of the protective mucus layer on the vaginal epithelium by hydrolysis of sialic acid on the glycans of mucous membranes. This process possibly facilitates adhesion of bacterial cells on the epithelium since it has been linked with the development of biofilm in other pathogenic conditions...
2017: PloS One
https://www.readbyqxmd.com/read/27914001/subtilosin-prevents-biofilm-formation-by-inhibiting-bacterial-quorum-sensing
#11
Ammar Algburi, Saskia Zehm, Victoria Netrebov, Anzhelica B Bren, Vladimir Chistyakov, Michael L Chikindas
Subtilosin, the cyclic lantibiotic protein produced by Bacillus subtilis KATMIRA1933, targets the surface receptor and electrostatically binds to the bacterial cell membrane. In this study, subtilosin was purified using ammonium sulfate ((NH4)2SO4) precipitation and purified via column chromatography. Subtilosin's antibacterial minimum and sub-minimum inhibitory concentrations (MIC and sub-MIC) and anti-biofilm activity (biofilm prevention) were established. Subtilosin was evaluated as a quorum sensing (QS) inhibitor in Gram-positive bacteria using Fe(III) reduction assay...
December 2, 2016: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/27845497/lysozyme-as-a-cotreatment-during-antibiotics-use-against-vaginal-infections-an-in-vitro-study-on-gardnerella-vaginalis-biofilm-models
#12
Olivier Thellin, Willy Zorzi, Danièle Zorzi, Philippe Delvenne, Ernst Heinen, Benaïssa ElMoualij, Pascale Quatresooz
Bacterial vaginoses are frequent in women, most of them involving Gardnerella vaginalis. In more than 50% of the cases, usual antibiotic treatments are not capable of eliminating completely the infection, leading to recurrent vaginosis. In addition to the appearance of antibiotic resistance, recurrence can be due to the development of a biofilm by G. vaginalis. In vitro experiments on G. vaginalis biofilms showed that the biofilm protected bacteria from the antibiotic clindamycin. Also, recombinant human lysozyme (rhLys) was able to both degrade biofilms and prevent their formation...
June 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/27161352/vaginal-microbiota
#13
REVIEW
Werner Mendling
The knowledge about the normal and abnormal vaginal microbiome has changed over the last years. Culturing techniques are not suitable any more for determination of a normal or abnormal vaginal microbiota. Non culture-based modern technologies revealed a complex and dynamic system mainly dominated by lactobacilli.The normal and the abnormal vaginal microbiota are complex ecosystems of more than 200 bacterial species influenced by genes, ethnic background and environmental and behavioral factors. Several species of lactobacilli per individuum dominate the healthy vagina...
2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27111438/screening-of-compounds-against-gardnerella-vaginalis-biofilms
#14
Cornelia Gottschick, Szymon P Szafranski, Brigitte Kunze, Helena Sztajer, Clarissa Masur, Christoph Abels, Irene Wagner-Döbler
Bacterial vaginosis (BV) is a common infection in reproductive age woman and is characterized by dysbiosis of the healthy vaginal flora which is dominated by Lactobacilli, followed by growth of bacteria like Gardnerella vaginalis. The ability of G. vaginalis to form biofilms contributes to the high rates of recurrence that are typical for BV and which unfortunately make repeated antibiotic therapy inevitable. Here we developed a biofilm model for G. vaginalis and screened a large spectrum of compounds for their ability to prevent biofilm formation and to resolve an existing G...
2016: PloS One
https://www.readbyqxmd.com/read/26965870/a-fruitful-alliance-the-synergy-between-atopobium-vaginae-and-gardnerella-vaginalis-in-bacterial-vaginosis-associated-biofilm
#15
Liselotte Hardy, Vicky Jespers, Said Abdellati, Irith De Baetselier, Lambert Mwambarangwe, Viateur Musengamana, Janneke van de Wijgert, Mario Vaneechoutte, Tania Crucitti
OBJECTIVES: Bacterial vaginosis (BV) is characterised by a change in the microbial composition of the vagina. The BV-associated organisms outnumber the health-associated Lactobacillus species and form a polymicrobial biofilm on the vaginal epithelium, possibly explaining the difficulties with antibiotic treatment. A better understanding of vaginal biofilm with emphasis on Atopobium vaginae and Gardnerella vaginalis may contribute to a better diagnosis and treatment of BV. METHODS: To this purpose, we evaluated the association between the presence of both bacteria by fluorescence in situ hybridisation (FISH) and BV by Nugent scoring in 463 vaginal slides of 120 participants participating in a clinical trial in Rwanda...
March 10, 2016: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/26782142/escherichia-coli-and-enterococcus-faecalis-are-able-to-incorporate-and-enhance-a-pre-formed-gardnerella-vaginalis-biofilm
#16
Joana Castro, Daniela Machado, Nuno Cerca
Gardnerella vaginalis is the most frequent microorganism found in bacterial vaginosis (BV), while Escherichia coli and Enterococcus faecalis are amongst the most frequent pathogens found in urinary tract infections (UTIs). This study aimed to evaluate possible interactions between UTIs pathogens and G. vaginalis using an in vitro dual-species biofilm model. Our results showed that dual-species biofilms reached significantly higher bacterial concentration than monospecies biofilms. Moreover, visualization of dual-populations species in the biofilms, using the epifluorescence microscopy, revealed that all of the urogenital pathogens coexisted with G...
April 2016: Pathogens and Disease
https://www.readbyqxmd.com/read/26652855/lactobacillus-crispatus-inhibits-growth-of-gardnerella-vaginalis-and-neisseria-gonorrhoeae-on-a-porcine-vaginal-mucosa-model
#17
Laura M Breshears, Vonetta L Edwards, Jacques Ravel, Marnie L Peterson
BACKGROUND: The vaginal microbiota can impact the susceptibility of women to bacterial vaginosis (BV) and sexually transmitted infections (STIs). BV is characterized by depletion of Lactobacillus spp., an overgrowth of anaerobes (often dominated by Gardnerella vaginalis) and a pH > 4.5. BV is associated with an increased risk of acquiring STIs such as chlamydia and gonorrhea. While these associations have been identified, the molecular mechanism(s) driving the risk of infections are unknown...
December 9, 2015: BMC Microbiology
https://www.readbyqxmd.com/read/26505928/bv-and-non-bv-associated-gardnerella-vaginalis-establish-similar-synergistic-interactions-with-other-bv-associated-microorganisms-in-dual-species-biofilms
#18
COMPARATIVE STUDY
Joana Castro, Nuno Cerca
Dual-species biofilm formation between Gardnerella vaginalis strains isolated from women with or without bacterial vaginosis (BV) and other 24 BV-associated microorganisms support that the key difference in virulence potential between BV-negative and BV-positive G. vaginalis strains seems not to be related with biofilm maturation.
December 2015: Anaerobe
https://www.readbyqxmd.com/read/26381661/optimization-of-culture-conditions-for-gardnerella-vaginalis-biofilm-formation
#19
Daniela Machado, Ana Palmeira-de-Oliveira, Nuno Cerca
Bacterial vaginosis is the leading vaginal disorder in women in reproductive age. Although bacterial vaginosis is related with presence of a biofilm composed predominantly by Gardnerella vaginalis, there has not been a detailed information addressing the environmental conditions that influence the biofilm formation of this bacterial species. Here, we evaluated the influence of some common culture conditions on G. vaginalis biofilm formation, namely inoculum concentration, incubation period, feeding conditions and culture medium composition...
November 2015: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/26305575/unravelling-the-bacterial-vaginosis-associated-biofilm-a-multiplex-gardnerella-vaginalis-and-atopobium-vaginae-fluorescence-in-situ-hybridization-assay-using-peptide-nucleic-acid-probes
#20
Liselotte Hardy, Vicky Jespers, Nassira Dahchour, Lambert Mwambarangwe, Viateur Musengamana, Mario Vaneechoutte, Tania Crucitti
Bacterial vaginosis (BV), a condition defined by increased vaginal discharge without significant inflammation, is characterized by a change in the bacterial composition of the vagina. Lactobacillus spp., associated with a healthy vaginal microbiome, are outnumbered by BV-associated organisms. These bacteria could form a polymicrobial biofilm which allows them to persist in spite of antibiotic treatment. In this study, we examined the presence of Gardnerella vaginalis and Atopobium vaginae in vaginal biofilms using Peptide Nucleic Acid (PNA) probes targeting these bacteria...
2015: PloS One
keyword
keyword
70956
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"