Jan Dreyer, Giulia Ricci, Jeroen van den Berg, Vivek Bhardwaj, Janina Funk, Claire Armstrong, Vincent van Batenburg, Chance Sine, Michael A Vaninsberghe, Richard Marsman, Imke K Mandemaker, Simone di Sanzo, Juliette Costantini, Stefano Manzo, Alva Biran, Claire Burny, Moritz Voelker-Albert, Anja Groth, Sabrina L Spencer, Alexander van Oudenaarden, Francesca Mattiroli
Long-term perturbation of de novo chromatin assembly during DNA replication has profound effects on epigenome maintenance and cell fate. The early mechanistic origin of these defects is unknown. Here, we combine acute degradation of Chromatin Assembly Factor 1 (CAF-1), a key player in de novo chromatin assembly, with single-cell genomics, quantitative proteomics, and live-microscopy to uncover these initiating mechanisms in human cells. CAF-1 loss immediately slows down DNA replication speed and renders nascent DNA hyper-accessible...
March 27, 2024: bioRxiv