Dimitar Angelov, Vladimir A Bondarenko, Sébastien Almagro, Hervé Menoni, Fabien Mongélard, Fabienne Hans, Flore Mietton, Vasily M Studitsky, Ali Hamiche, Stefan Dimitrov, Philippe Bouvet
Remodeling machines play an essential role in the control of gene expression, but how their activity is regulated is not known. Here we report that the nuclear protein nucleolin possesses a histone chaperone activity and that this factor greatly enhances the activity of the chromatin remodeling machineries SWI/SNF and ACF. Interestingly, nucleolin is able to induce the remodeling by SWI/SNF of macroH2A, but not of H2ABbd nucleosomes, which are otherwise resistant to remodeling. This new histone chaperone promotes the destabilization of the histone octamer, helping the dissociation of a H2A-H2B dimer, and stimulates the SWI/SNF-mediated transfer of H2A-H2B dimers...
April 19, 2006: EMBO Journal