Charlotte Hodson, Jason K K Low, Sylvie van Twest, Samuel E Jones, Paolo Swuec, Vincent Murphy, Kaima Tsukada, Matthew Fawkes, Rohan Bythell-Douglas, Adelina Davies, Jessica K Holien, Julienne J O'Rourke, Benjamin L Parker, Astrid Glaser, Michael W Parker, Joel P Mackay, Andrew N Blackford, Alessandro Costa, Andrew J Deans
The RecQ-like helicase BLM cooperates with topoisomerase IIIα, RMI1, and RMI2 in a heterotetrameric complex (the "Bloom syndrome complex") for dissolution of double Holliday junctions, key intermediates in homologous recombination. Mutations in any component of the Bloom syndrome complex can cause genome instability and a highly cancer-prone disorder called Bloom syndrome. Some heterozygous carriers are also predisposed to breast cancer. To understand how the activities of BLM helicase and topoisomerase IIIα are coupled, we purified the active four-subunit complex...
February 8, 2022: Proceedings of the National Academy of Sciences of the United States of America