keyword
MENU ▼
Read by QxMD icon Read
search

Resolvase

keyword
https://www.readbyqxmd.com/read/27908236/interaction-between-saccharomyces-cerevisiae-mitochondrial-dna-binding-protein-abf2p-and-cce1p-resolvase
#1
E O Samoilova, I A Krasheninnikov, S A Levitskii
Mitochondrial DNA is susceptible to the action of reactive oxygen species generated by the reactions of oxidative phosphorylation. Homologous recombination is one of the mechanisms providing integrity of the mitochondrial genome. Some proteins that take part in this process in budding yeast mitochondria have been identified. These include Abf2p, the major protein of the mt-nucleoid that specifically binds cruciform DNA, and Cce1p - Holliday junction resolvase. Here we show that Abf2p does not significantly affect either binding of Cce1p to branched DNA or rate and specificity of Holliday junction resolution...
October 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27891116/complete-genome-sequence-of-clostridium-estertheticum-dsm-8809-a-microbe-identified-in-spoiled-vacuum-packed-beef
#2
Zhongyi Yu, Lynda Gunn, Evan Brennan, Rachael Reid, Patrick G Wall, Peadar Ó Gaora, Daniel Hurley, Declan Bolton, Séamus Fanning
Blown pack spoilage (BPS) is a major issue for the beef industry. Etiological agents of BPS involve members of a group of Clostridium species, including Clostridium estertheticum which has the ability to produce gas, mostly carbon dioxide, under anaerobic psychotrophic growth conditions. This spore-forming bacterium grows slowly under laboratory conditions, and it can take up to 3 months to produce a workable culture. These characteristics have limited the study of this commercially challenging bacterium. Consequently information on this bacterium is limited and no effective controls are currently available to confidently detect and manage this production risk...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27855779/local-chromosome-context-is-a-major-determinant-of-crossover-pathway-biochemistry-during-budding-yeast-meiosis
#3
Darpan Medhi, Alastair Sh Goldman, Michael Lichten
The budding yeast genome contains regions where meiotic recombination initiates more frequently than in others. This pattern parallels enrichment for the meiotic chromosome axis proteins Hop1 and Red1. These proteins are important for Spo11-catalyzed double strand break formation; their contribution to crossover recombination remains undefined. Using the sequence-specific VMA1-derived endonuclease (VDE) to initiate recombination in meiosis, we show that chromosome structure influences the choice of proteins that resolve recombination intermediates to form crossovers...
November 18, 2016: ELife
https://www.readbyqxmd.com/read/27806301/meiotic-nuclear-oscillations-are-necessary-to-avoid-excessive-chromosome-associations
#4
Mariola R Chacón, Petrina Delivani, Iva M Tolić
Pairing of homologous chromosomes is a crucial step in meiosis, which in fission yeast depends on nuclear oscillations. However, how nuclear oscillations help pairing is unknown. Here, we show that homologous loci typically pair when the spindle pole body is at the cell pole and the nucleus is elongated, whereas they unpair when the spindle pole body is in the cell center and the nucleus is round. Inhibition of oscillations demonstrated that movement is required for initial pairing and that prolonged association of loci leads to mis-segregation...
November 1, 2016: Cell Reports
https://www.readbyqxmd.com/read/27779184/replication-intermediates-that-escape-dna2-activity-are-processed-by-holliday-junction-resolvase-yen1
#5
Gizem Ölmezer, Maryna Levikova, Dominique Klein, Benoît Falquet, Gabriele Alessandro Fontana, Petr Cejka, Ulrich Rass
Cells have evolved mechanisms to protect, restart and repair perturbed replication forks, allowing full genome duplication, even under replication stress. Interrogating the interplay between nuclease-helicase Dna2 and Holliday junction (HJ) resolvase Yen1, we find the Dna2 helicase activity acts parallel to homologous recombination (HR) in promoting DNA replication and chromosome detachment at mitosis after replication fork stalling. Yen1, but not the HJ resolvases Slx1-Slx4 and Mus81-Mms4, safeguards chromosome segregation by removing replication intermediates that escape Dna2...
October 25, 2016: Nature Communications
https://www.readbyqxmd.com/read/27565373/reduced-kinase-activity-of-polo-kinase-cdc5-affects-chromosome-stability-and-dna-damage-response-in-s-cerevisiae
#6
Chetan C Rawal, Sara Riccardo, Chiara Pesenti, Matteo Ferrari, Federica Marini, Achille Pellicioli
Polo-like kinases (PLKs) control several aspects of eukaryotic cell division and DNA damage response. Remarkably, PLKs are overexpressed in several types of cancer, being therefore a marker of bad prognosis. As such, specific PLK kinase activity inhibitors are already used in clinical trials and the regulation of PLK activation is a relevant topic of cancer research. Phosphorylation of threonine residues in the T-loop of the kinase domain is pivotal for PLKs activation. Here, we show that T238A substitution in the T-loop reduces the kinase activity of Cdc5, the only PLK in Saccharomyces cerevisiae, with minor effect on cell growth in unperturbed conditions...
November 2016: Cell Cycle
https://www.readbyqxmd.com/read/27527105/novel-heterologous-bacterial-system-reveals-enhanced-susceptibility-to-dna-damage-mediated-by-yqgf-a-nearly-ubiquitous-and-often-essential-gene
#7
Dylan J McClung, Abigail Calixto, Mackenzie N Mosera, Raagni Kumar, Ellen L Neidle, Kathryn T Elliott
Despite its presence in most bacteria, yqgF remains one of only 13 essential genes of unknown function in Escherichia coli. Predictions of YqgF function often derive from sequence similarity to RuvC, the canonical Holliday junction resolvase. To clarify its role, we deleted yqgF from a bacterium where it is not essential, Acinetobacter baylyi ADP1. Loss of yqgF impaired growth and increased the frequency of transformation and allelic replacement (TAR). When E. coli yqgF was inserted in place of its A. baylyi chromosomal orthologue, wild-type growth and TAR were restored...
October 2016: Microbiology
https://www.readbyqxmd.com/read/27407146/single-molecule-imaging-reveals-a-common-mechanism-shared-by-g-quadruplex-resolving-helicases
#8
Ramreddy Tippana, Helen Hwang, Patricia L Opresko, Vilhelm A Bohr, Sua Myong
G-quadruplex (GQ) is a four stranded DNA secondary structure that arises from a guanine rich sequence. Stable formation of GQ in genomic DNA can be counteracted by the resolving activity of specialized helicases including RNA helicase AU (associated with AU rich elements) (RHAU) (G4 resolvase 1), Bloom helicase (BLM), and Werner helicase (WRN). However, their substrate specificity and the mechanism involved in GQ unfolding remain uncertain. Here, we report that RHAU, BLM, and WRN exhibit distinct GQ conformation specificity, but use a common mechanism of repetitive unfolding that leads to disrupting GQ structure multiple times in succession...
July 26, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27383418/gen1-and-eme1-play-redundant-roles-in-dna-repair-and-meiotic-recombination-in-mice
#9
Xiaowen Wang, Herui Wang, Bin Guo, Ya Zhang, Yinv Gong, Chi Zhang, Hong Xu, Xiaohui Wu
Resolution of the Holliday junction (HJ) is essential for homologous recombination and DNA repair. In Saccharomyces cerevisiae, HJ resolvase Yen1 and the Mus81-Mms4 complex are redundant in DNA damage repair. In cultured mammalian cells, such redundancy also exists between Yen1 ortholog GEN1 and the Mus81-Mms1 ortholog MUS81-EME1. In this report, we further tested if GEN1 and EME1 redundantly affect HJ-related physiological processes in mice. We found that combined homozygous mutations of Gen1 and Eme1 led to synthetic lethality during early embryonic stages...
July 6, 2016: DNA and Cell Biology
https://www.readbyqxmd.com/read/27345943/mycobacterium-tuberculosis-ruvx-is-a-holliday-junction-resolvase-formed-by-dimerisation-of-the-monomeric-yqgf-nuclease-domain
#10
Astha Nautiyal, P Sandhya Rani, Gary J Sharples, K Muniyappa
No abstract text is available yet for this article.
July 2016: Molecular Microbiology
https://www.readbyqxmd.com/read/27284361/effect-of-gen1-interference-on-the-chemosensitivity-of-the-breast-cancer-mcf-7-and-skbr3-cell-lines
#11
Yunlu Wu, Ying Qian, Guozhong Zhou, Juan Lv, Qiuyue Yan, Xuejun Dong
Chemotherapy is a notable method for the treatment of breast cancer. Numerous genes associated with the sensitivity of cancer to chemotherapy have been found. In recent years, evidence has suggested that a particular structure termed Holliday junction (HJ) plays a crucial role in cancer chemosensitivity. Targeting HJ resolvases, such as structure-specific endonuclease subunit SLX4 (Slx4) and MUS81 structure-specific endonuclease subunit (Mus81), significantly increases the chemosensitivity of tumor cells. Flap endonuclease GEN homolog 1 (GEN1) is a HJ resolvase that belongs to the Rad2/xeroderma pigmentosum complementation group G nuclease family...
June 2016: Oncology Letters
https://www.readbyqxmd.com/read/27245214/nucleo-cytoplasmic-shuttling-of-the-endonuclease-ankyrin-repeats-and-lem-domain-containing-protein-1-ankle1-is-mediated-by-canonical-nuclear-export-and-nuclear-import-signals
#12
Livija Zlopasa, Andreas Brachner, Roland Foisner
BACKGROUND: Ankyrin repeats and LEM domain containing protein 1 (Ankle1) belongs to the LEM protein family, whose members share a chromatin-interacting LEM motif. Unlike most other LEM proteins, Ankle1 is not an integral protein of the inner nuclear membrane but shuttles between the nucleus and the cytoplasm. It contains a GIY-YIG-type nuclease domain, but its function is unknown. The mammalian genome encodes only one other GIY-YIG domain protein, termed Slx1. Slx1 has been described as a resolvase that processes Holliday junctions during homologous recombination-mediated DNA double strand break repair...
2016: BMC Cell Biology
https://www.readbyqxmd.com/read/27242758/proteomic-analysis-of-a-novel-bacillus-jumbo-phage-revealing-glycoside-hydrolase-as-structural-component
#13
Yihui Yuan, Meiying Gao
Tailed phages with genomes of larger than 200 kbp are classified as Jumbo phages and exhibited extremely high uncharted diversity. The genomic annotation of Jumbo phage is often disappointing because most of the predicted proteins, including structural proteins, failed to make good hits to the sequences in the databases. In this study, 23 proteins of a novel Bacillus Jumbo phage, vB_BpuM_BpSp, were identified as phage structural proteins by the structural proteome analysis, including 14 proteins of unknown function, 5 proteins with predicted function as structural proteins, a glycoside hydrolase, a Holliday junction resolvase, a RNA-polymerase β-subunit, and a host-coding portal protein, which might be hijacked from the host strain during phage virion assembly...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27147534/improvement-of-dna-minicircle-production-by-optimization-of-the-secondary-structure-of-the-5-utr-of-para-resolvase
#14
Michaela Šimčíková, Cláudia P A Alves, Liliana Brito, Kristala L J Prather, Duarte M F Prazeres, Gabriel A Monteiro
The use of minicircles in gene therapy applications is dependent on the availability of high-producer cell systems. In order to improve the performance of minicircle production in Escherichia coli by ParA resolvase-mediated in vivo recombination, we focus on the 5' untranslated region (5'-UTR) of parA messenger RNA (mRNA). The arabinose-inducible PBAD/araC promoter controls ParA expression and strains with improved arabinose uptake are used. The 27-nucleotide-long 5'-UTR of parA mRNA was optimized using a predictive thermodynamic model...
August 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27131360/the-borrelia-burgdorferi-telomere-resolvase-rest-anneals-ssdna-complexed-with-its-cognate-ssdna-binding-protein
#15
Shu Hui Huang, Kerri Kobryn
Spirochetes of the genus Borrelia possess unusual genomes that consist in a linear chromosome and multiple linear and circular plasmids. The linear replicons are terminated by covalently closed hairpin ends, referred to as hairpin telomeres. The hairpin telomeres represent a simple solution to the end-replication problem. Deoxyribonucleic acid replication initiates internally and proceeds bidirectionally toward the hairpin telomeres. The telomere resolvase, ResT, forms the hairpin telomeres from replicated telomere intermediates in a reaction with similarities to those promoted by type IB topoisomerases and tyrosine recombinases...
June 20, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27101788/complete-nucleotide-sequence-of-ph11-an-inchi2-plasmid-conferring-multi-antibiotic-resistance-and-multi-heavy-metal-resistance-genes-in-a-clinical-klebsiella-pneumoniae-isolate
#16
Yao Zhai, Zilong He, Yu Kang, Haiying Yu, Jianfeng Wang, Pengcheng Du, Zhao Zhang, Songnian Hu, Zhancheng Gao
The complete 284,628bp sequence of pH11, an IncHI2 plasmid, was determined through single-molecule, real-time (SMRT) sequencing. Harbored by a clinical Klebsiella pneumoniae strain H11, and isolated in Beijing, this plasmid contains multiple antibiotic resistance genes, including catA2, aac(6')-Ib, strB, strA, dfrA19, blaTEM-1, blaSHV-12, sul1, qacE delta 1, ereA, arr2, and aac3. The aac(6')-Ib is carried by a class I integron. Plasmid pH11 also carries several genes associated with resistance to heavy metals, such as tellurium, mercury, cobalt, zinc, nickel, copper, lead and cadmium...
July 2016: Plasmid
https://www.readbyqxmd.com/read/27038615/25%C3%A2-years-on-and-no-end-in-sight-a-perspective-on-the-role-of-recg-protein
#17
Robert G Lloyd, Christian J Rudolph
The RecG protein of Escherichia coli is a double-stranded DNA translocase that unwinds a variety of branched substrates in vitro. Although initially associated with homologous recombination and DNA repair, studies of cells lacking RecG over the past 25 years have led to the suggestion that the protein might be multi-functional and associated with a number of additional cellular processes, including initiation of origin-independent DNA replication, the rescue of stalled or damaged replication forks, replication restart, stationary phase or stress-induced 'adaptive' mutations and most recently, naïve adaptation in CRISPR-Cas immunity...
April 2, 2016: Current Genetics
https://www.readbyqxmd.com/read/27013661/structure-and-metal-binding-properties-of-a-poxvirus-resolvase
#18
Huiguang Li, Young Hwang, Kay Perry, Frederic Bushman, Gregory D Van Duyne
Poxviruses replicate their linear genomes by forming concatemers that must be resolved into monomeric units to produce new virions. A viral resolvase cleaves DNA four-way junctions extruded at the concatemer junctions to produce monomeric genomes. This cleavage reaction is required for viral replication, so the resolvase is an attractive target for small molecule inhibitors. To provide a platform for understanding resolvase mechanism and designing inhibitors, we have determined the crystal structure of the canarypox virus (CPV) resolvase...
May 20, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27010650/the-smc-5-6-complex-and-the-him-6-blm-helicase-synergistically-promote-meiotic-recombination-intermediate-processing-and-chromosome-maturation-during-caenorhabditis-elegans-meiosis
#19
Ye Hong, Remi Sonneville, Ana Agostinho, Bettina Meier, Bin Wang, J Julian Blow, Anton Gartner
Meiotic recombination is essential for the repair of programmed double strand breaks (DSBs) to generate crossovers (COs) during meiosis. The efficient processing of meiotic recombination intermediates not only needs various resolvases but also requires proper meiotic chromosome structure. The Smc5/6 complex belongs to the structural maintenance of chromosome (SMC) family and is closely related to cohesin and condensin. Although the Smc5/6 complex has been implicated in the processing of recombination intermediates during meiosis, it is not known how Smc5/6 controls meiotic DSB repair...
March 2016: PLoS Genetics
https://www.readbyqxmd.com/read/26983858/horizontal-transfer-of-%C3%AE-carbonic-anhydrase-genes-from-prokaryotes-to-protozoans-insects-and-nematodes
#20
Reza Zolfaghari Emameh, Harlan R Barker, Martti E E Tolvanen, Seppo Parkkila, Vesa P Hytönen
BACKGROUND: Horizontal gene transfer (HGT) is a movement of genetic information occurring outside of normal mating activities. It is especially common between prokaryotic endosymbionts and their protozoan, insect, and nematode hosts. Although beta carbonic anhydrase (β-CA) plays a crucial role in metabolic functions of many living organisms, the origin of β-CA genes in eukaryotic species remains unclear. METHODS: This study was conducted using phylogenetics, prediction of subcellular localization, and identification of β-CA, transposase, integrase, and resolvase genes on the MGEs of bacteria...
2016: Parasites & Vectors
keyword
keyword
70916
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"