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Milk phosphate

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https://www.readbyqxmd.com/read/28712055/potential-of-casein-as-a-carrier-for-biologically-active-agents
#1
REVIEW
Tomasz Konrad Głąb, Janusz Boratyński
Casein is the collective name for a family of milk proteins. In bovine milk, casein comprises four peptides: αS1, αS2, β, and κ, differing in their amino acid, phosphorus and carbohydrate content but similar in their amphiphilic character. Hydrophilic and hydrophobic regions of casein show block distribution in the protein chain. Casein peptides carry negative charge on their surface as a result of phosphorylation and tend to bind nanoclusters of amorphous calcium phosphate. Due to these properties, in suitable conditions, casein molecules agglomerate into spherical micelles...
August 2017: Topics in Current Chemistry (Journal)
https://www.readbyqxmd.com/read/28690057/effects-of-depleting-ionic-strength-on-31-p-nuclear-magnetic-resonancespectra-of-micellar-casein-during-membrane-separation-and-diafiltration-of-skim-milk
#2
Mattia Boiani, Padraig McLoughlin, Mark A E Auty, Richard J FitzGerald, Phil M Kelly
Membrane separation processes used in the concentration and isolation of micellar casein-based milk proteins from skim milk rely on extensive permeation of its soluble serum constituents, especially lactose and minerals. Whereas extensive literature exists on how these processes influence the gross composition of milk proteins, we have little understanding of the effects of such ionic depletion on the core structural unit of micellar casein [i.e., the casein phosphate nanocluster (CPN)]. The (31)P nuclear magnetic resonance (NMR) is an analytical technique that is capable of identifying soluble and organic forms of phosphate in milk...
July 6, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28651380/utilization-of-acid-pre-treated-coconut-dregs-as-a-substrate-for-production-of-detergent-compatible-lipase-by-bacillus-stratosphericus
#3
Nur Bainun Mohd Zin, Busyra Mohamad Yusof, Siti Nurbaya Oslan, Helmi Wasoh, Joo Shun Tan, Arbakariya B Ariff, Murni Halim
In recent years, many efforts have been directed to explore the methods to reduce the production costs of industrial lipase by improving the yield and the use of low-cost agricultural wastes. Coconut dregs, which is a lignocellulosic by-product from coconut oil and milk processing plants, is rich in cellulose (36%) and crude fat (9%). A newly isolated Bacillus stratosphericus has been demonstrated to perform cellulose hydrolysis on coconut dregs producing fermentable sugars. The highest extracellular lipase activity of 140 U/mL has been achieved in submerged fermentation with acid pre-treated coconut dregs...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28647329/structural-changes-induced-by-high-pressure-processing-in-micellar-casein-and-milk-protein-concentrates
#4
Lee Cadesky, Markus Walkling-Ribeiro, Kyle T Kriner, Mukund V Karwe, Carmen I Moraru
Reconstituted micellar casein concentrates and milk protein concentrates of 2.5 and 10% (wt/vol) protein concentration were subjected to high-pressure processing at pressures from 150 to 450 MPa, for 15 min, at ambient temperature. The structural changes induced in milk proteins by high-pressure processing were investigated using a range of physical, physicochemical, and chemical methods, including dynamic light scattering, rheology, mid-infrared spectroscopy, scanning electron microscopy, proteomics, and soluble mineral analyses...
June 21, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28628402/calcium-bioavailability-from-mineral-waters-with-different-mineralization-in-comparison-to-milk-and-a-supplement
#5
Theresa Greupner, Inga Schneider, Andreas Hahn
OBJECTIVE: The aim of the present study was to compare the bioavailability of calcium from 3 mineral waters with different concentrations of minerals with that of milk and a calcium supplement. METHODS: A single-center, randomized controlled trial with a crossover design with 21 healthy men and women was conducted at the Institute of Food Science and Human Nutrition, Leibniz University Hannover. The participants consumed the 5 test products providing 300 mg of calcium each on 5 examination days with 1-week wash-out phases in between...
July 2017: Journal of the American College of Nutrition
https://www.readbyqxmd.com/read/28620248/relative-gene-expression-of-fatty-acid-synthesis-genes-at-60-days-postpartum-in-bovine-mammary-epithelial-cells-of-surti-and-jafarabadi-buffaloes
#6
Mamta Janmeda, Vishnu Kharadi, Gaurav Pandya, Balkrishna Brahmkshtri, Umed Ramani, Kuldeep Tyagi
AIM: Aim of the study was to study the relative gene expression of genes associated with fatty acid synthesis at 60 days postpartum (pp) in bovine mammary epithelial cells (MECs) of Surti and Jafarabadi buffaloes. MATERIALS AND METHODS: A total of 10 healthy Surti and Jafarabadi buffaloes of each breed were selected at random from Livestock Research Station, Navsari and Cattle Breeding Farm, Junagadh, Gujarat, respectively, for this study. Milk sample was collected from each selected buffalo at day 60 pp from these two breeds to study relative gene expression of major milk fat genes using non-invasive approach of obtaining primary bovine MECs (pBMEC) from milk samples...
May 2017: Veterinary World
https://www.readbyqxmd.com/read/28601327/milk-fat-globule-epidermal-growth-factor-factor-viii-attenuates-sepsis-induced-acute-kidney-injury
#7
Cindy Cen, Monowar Aziz, Weng-Lang Yang, Mian Zhou, Jeffrey M Nicastro, Gene F Coppa, Ping Wang
BACKGROUND: Acute kidney injury (AKI) is most commonly caused by sepsis in critically ill patients, and it is associated with high morbidity and mortality. The pathophysiology of sepsis-induced AKI is generally accepted to include direct inflammatory injury, endothelial cell dysfunction, and apoptosis. Milk fat globule-epidermal growth factor-factor VIII (MFG-E8) is a secretory glycoprotein with a known role in the enhancement of apoptotic cell clearance and regulation of inflammation...
June 1, 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/28594955/inorganic-salts-and-intracellular-polyphosphate-inclusions-play-a-role-in-the-thermotolerance-of-the-immunobiotic-lactobacillus-rhamnosus-crl-1505
#8
María A Correa Deza, Mariana Grillo-Puertas, Susana Salva, Viviana A Rapisarda, Carla L Gerez, Graciela Font de Valdez
In this work, the thermotolerance of Lactobacillus rhamnosus CRL1505, an immunobiotic strain, was studied as a way to improve the tolerance of the strain to industrial processes involving heat stress. The strain displayed a high intrinsic thermotolerance (55°C, 20 min); however, after 5 min at 60°C in phosphate buffer a two log units decrease in cell viability was observed. Different heat shock media were tested to improve the cell survival. Best results were obtained in the mediumcontaining inorganic salts (KH2PO4, Na2HPO4, MnSO4, and MgSO4) likely as using 10% skim milk...
2017: PloS One
https://www.readbyqxmd.com/read/28559627/physico-chemical-functional-and-rheological-properties-of-milk-protein-concentrate-60-as-affected-by-disodium-phosphate-addition-diafiltration-and-homogenization
#9
Ganga Sahay Meena, Ashish Kumar Singh, Sumit Arora, Sanket Borad, Rajan Sharma, Vijay Kumar Gupta
Ultrafiltration and diafiltration of skim milk altered delicate salt equilibrium and composition of 5× UF retentate (5× UFR), and thus adversely affected the reconstitutional and functional properties of milk protein concentrate (MPC) powders. It might be due to interaction and aggregation of proteins during spray drying. Therefore, this study was envisaged to investigate the effect of disodium phosphate (DSP) addition, diafiltration and homogenization of retentates on physico-chemical, functional and rheological properties of MPC60 powders...
May 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28524011/effects-of-heating-and-calcium-and-phosphate-mineral-supplementation-on-the-physical-properties-of-rennet-induced-coagulation-of-camel-and-cow-milk-gels
#10
Mohammad Kamal, Mohammed Foukani, Romdhane Karoui
The physical properties of rennet-induced coagulation of preheated camel and cow milk gels (50 and 70 °C for 10 min) enriched with calcium chloride (CaCl2) and hydrogen phosphate dihydrate (Na2HPO42H2O) were evaluated using the dynamic low amplitude oscillatory shear analysis. The storage modulus (G') and loss modulus (G") of camel milk gels showed significant (P < 0·05) lower values than those of cow milk gels. The preheating of camel milk at 50 °C affected negatively the gelation properties, while the preheating at 70 °C prevented the formation of rennet-induced milk gels...
May 2017: Journal of Dairy Research
https://www.readbyqxmd.com/read/28493372/glycosuria-and-hyperglycemia-in-the-neonatal-period-as-the-first-clinical-sign-of-fanconi-bickel-syndrome
#11
María Pilar Bahíllo-Curieses, Rebeca Garrote-Molpeceres, María Miñambres-Rodríguez, M Rosa Del Real-Llorente, Cristina Tobar-Mideros, Sara Rellán-Rodríguez
Fanconi-Bickel syndrome is a rare inherited disease characterized by the combination of hepatorenal glycogen accumulation, proximal renal tubular dysfunction and impaired utilization of glucose and galactose. The first symptoms of the disorder are recognized in late infancy as clinical characteristics appear. Therapeutic approach is mainly conservative with supplements of calcium, phosphate and vitamin D and small frequent feedings to avoid hypoglycemia. We report 1 clinical case of very early diagnosis, a 19 days old baby girl, in which the first clinical sign of the disease was the detection of glycosuria and vomits...
May 11, 2017: Pediatric Diabetes
https://www.readbyqxmd.com/read/28493294/commercial-mineral-enhanced-dairy-by-products-as-sodium-replacers-antioxidants-and-calcium-fortifiers-in-sausages
#12
Anne Maren Engeloug, Gu Yi, Bjørg Egelandsdal, Anna Haug, Berit Nordvi
Sausages are perceived as high in Na and with a too high Na:K ratio. Frankfurter type sausages are regarded as important contributors of sodium in the diet and thereby of health risks. Surplus products from the dairy industry are various mineral powders enriched in either potassium, calcium, or phosphate and include various amounts of lactose. Sausages were produced at 3 sodium levels (equivalent to 13, 15, and 17 g NaCl/kg sausage) using 4 different milk ingredients (a dried skimmed milk powder, a calcium enriched milk powder, a potassium enriched powder, and a lactose enriched powder)...
June 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28457551/short-communication-comparative-proteomic-analysis-of-the-lactating-and-nonlactating-bovine-mammary-gland
#13
W T Dai, Q J Wang, Y X Zou, R R White, J X Liu, H Y Liu
The objective of this study was to determine how bovine mammary protein profiles vary during lactation and the dry period. Three lactating and 3 nonlactating cows were selected for mammary gland tissue sampling. Compared with the mammary proteins in nonlactating cows, a total of 60 differentially expressed proteins (DEP, including 57 upregulated and 3 downregulated) were identified in lactating cows using 2-dimensional difference gel electrophoresis combined with mass spectrometry. These DEP included enzymes and proteins associated with various macromolecular metabolic processes, and appeared to promote the increased metabolic activity associated with milk synthesis and secretion...
July 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28434752/effects-of-intrauterine-infusion-of-escherichia-coli-lipopolysaccharide-on-uterine-health-resolution-of-purulent-vaginal-discharge-and-reproductive-performance-of-lactating-dairy-cows
#14
João G N Moraes, Paula R B Silva, Luís G D Mendonça, Alexandre A Scanavez, Joseane C C Silva, Ricardo C Chebel
The objectives of the current experiment were to evaluate the effects of intrauterine infusion of Escherichia coli lipopolysaccharide (LPS) in cows diagnosed with purulent vaginal discharge (PVD) on intrauterine cell population, resolution of PVD, uterine health, and reproductive performance. Jersey cows (n = 3,084) were examined using the Metricheck device to diagnose PVD at 35 ± 6 d postpartum. Purulent vaginal discharge was defined as the presence of purulent (≥50% pus) discharge detectable in the vagina...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28434743/the-effect-of-emulsifying-salts-on-the-turbidity-of-a-diluted-milk-system-with-varying-ph-and-protein-concentration
#15
M D Culler, Y Saricay, F M Harte
Solutions of 10 commonly used emulsifying salts (ES) listed in the Code of Federal Regulations (21CFR133.179) for pasteurized process cheese were tested for their effect on the turbidity of a diluted milk system at different pH and protein concentrations to characterize the conditions that affect micellar structure. Emulsifying salt solutions were made by mixing the ES in a 1-in-20 dilution of water in skim milk ultrafiltrate (3 kDa molecular weight cut-off) to obtain ES concentrations from 0 to 248 mM. Skim milk was added to solutions containing nanopure water, skim milk ultrafiltrate, and a specific ES ranging in concentration from 0 to 248 mM and pH 5, 5...
June 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28434729/effects-of-intrauterine-infusion-of-escherichia-coli-lipopolysaccharide-on-uterine-mrna-gene-expression-and-peripheral-polymorphonuclear-leukocytes-in-jersey-cows-diagnosed-with-purulent-vaginal-discharge
#16
João G N Moraes, Luís G D Mendonça, Paula R B Silva, Alexandre A Scanavez, Klibs N Galvão, Michael A Ballou, Milli Worku, Ricardo C Chebel
The objectives of the current experiment were to investigate the effects of intrauterine treatment of cows with purulent vaginal discharge (PVD) using lipopolysaccharide (LPS) from Escherichia coli on uterine mRNA expression of genes related to inflammatory responses, peripheral polymorphonuclear leukocyte (PMN) function, hematological parameters, and blood concentrations of cortisol, haptoglobin, and progesterone (P4). Jersey cows (n = 3,084) were examined for PVD at 35 ± 6 d postpartum using the Metricheck device (Simcro, Hamilton, New Zealand)...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28420530/rapid-determination-of-the-various-native-forms-of-vitamin-b6-and-b2-in-cow-s-milk-using-ultra-high-performance-liquid-chromatography
#17
A Schmidt, M G Schreiner, H K Mayer
As the formation of pyridoxal phosphate, the active cofactor of vitamin B6, is dependent on riboflavin 5-phosphate, we propose a fast and simple ultra-high performance liquid chromatography method for the simultaneous determination of the native B6 vitamers pyridoxal, pyridoxine, pyridoxamine, their mono phosphorus esters and 4-pyridoxic acid as well as vitamin B2 as riboflavin and its phosphorus ester riboflavin 5-phosphate in milk. Separation was achieved under 6.0min by reversed-phase and pH gradient elution...
April 9, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28403179/new-route-for-self-assembly-of-%C3%AE-lactalbumin-nanotubes-and-their-use-as-templates-to-grow-silver-nanotubes
#18
Wei-Chun Fu, Mauricio A Opazo, Sergio M Acuña, Pedro G Toledo
Nanotubes are formed by self-assembly of α-lactalbumin milk protein following a different route than established for the hydrolysis which involves V8 enzyme, phosphate buffer and appropriate amounts of calcium at neutral pH. The resulting nanotubes are used as templates for the growth of conductive silver nanotubes. TEM, SEM-EDS, AFM and FTIR are used for characterization.
2017: PloS One
https://www.readbyqxmd.com/read/28390667/pyridoxal-5-phosphate-mediated-preparation-of-immobilized-metal-affinity-material-for-highly-selective-and-sensitive-enrichment-of-phosphopeptides
#19
Qian Wang, Xiao-Mei He, Xi Chen, Gang-Tian Zhu, Ren-Qi Wang, Yu-Qi Feng
Phosphorylation is a crucial post-translational modification, which plays pivotal roles in various biological processes. Analysis of phosphopeptides by mass spectrometry (MS) is intractable on account of their low stoichiometry and the ion suppression from non-phosphopeptides. Thus, enrichment of phosphopeptides before MS analysis is indispensable. In this work, we employed pyridoxal 5'-phosphate (PLP), as an immobilized metal affinity chromatography (IMAC) ligand for the enrichment of phosphopeptides. PLP was grafted onto several substrates such as silica (SiO2), oxidized carbon nanotube (OCNT) and silica coated magnetic nanoparticles (Fe3O4@SiO2)...
May 26, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28342973/effects-of-a-sustained-release-formulation-of-1-25-dihydroxyvitamin-d3-glycosides-for-milk-fever-prevention-on-serum-1-25-dihydroxyvitamin-d3-calcium-and-phosphorus-in-dairy-cows
#20
Heinrich Bachmann, Michael Lanz, Susanne Kehrle, Wolfgang Bittner, Annick Toggenburger, Georg A Mathis, Walter Rambeck
Milk fever (MF) is a metabolic disease in dairy cows around parturition. The clinical lead sign is muscular paresis leading in severe cases to paralysis of the affected animal. Multiparturient animals of high performing dairy breeds are most likely to be affected and have a high probability of recurrence. An acute drop in blood calcium levels causes the disease when the demand for calcium at the onset of lactation exceeds the ability to replete blood calcium levels through mobilization from bone and intestinal uptake...
March 23, 2017: Journal of Steroid Biochemistry and Molecular Biology
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