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Milk phosphate

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https://www.readbyqxmd.com/read/29136806/multivariate-analysis-of-organic-acids-in-fermented-food-from-reversed-phase-high-performance-liquid-chromatography-data
#1
Pablo Mortera, Federico A Zuljan, Christian Magni, Santiago A Bortolato, Sergio H Alarcón
Multivariate calibration coupled to RP-HPLC with diode array detection (HPLC-DAD) was applied to the identification and the quantitative evaluation of the short chain organic acids (malic, oxalic, formic, lactic, acetic, citric, pyruvic, succinic, tartaric, propionic and α-cetoglutaric) in fermented food. The goal of the present study was to get the successful resolution of a system in the combined occurrence of strongly coeluting peaks, of distortions in the time sensors among chromatograms, and of the presence of unexpected compounds not included in the calibration step...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29136753/-metabolomics-study-of-tris-2-chloroethyl-phosphate-induced-hepaotoxicity-and-nephrotoxicity-in-sprague-dawley-rats
#2
W Q Yang, F Zhao, L Li, Y J Fang
Objective: To discuss the potential toxic target organ and the toxic effects and mechanisms of tris (2-chloroethyl) phosphate (TCEP) on SD rats. Methods: 40 female SD rats weaning from milk for 21 days, weighted (50±2.3)g were selected as subjects and marked by the weight. They were randomly divided into 4 groups, namely control group, 50 (L), 100 (M) and 250 (H) mg·kg(-1)·d(-1) dose of TCEP group. Each group has 10 rats, and administrated the corresponding dose of drug or vehicle by mouth, quaque die for 60 days...
November 6, 2017: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/29099901/-factors-influencing-the-quality-of-the-first-colostrum-in-holstein-friesian-cows-and-their-relationship-to-postpartum-calcium-concentrations-in-the-blood-and-colostrum
#3
Maria Karl, Rudolf Staufenbiel
OBJECTIVE: To investigate the factors that affect the quality (immunoglobulin concentration) of the first colostrum in Holstein Friesian cows and their relationship to the calcium concentrations in the blood and colostrum, with particular attention to the lactation number. MATERIAL AND METHODS: An observational study was conducted at a commercial dairy farm (2278 Holstein Friesian cows). The analysis comprised the following variables: colostrum content of immunoglobulins and calcium, colostrum yield; blood serum concentrations of calcium, phosphate, non-esterified fatty acids, and β-hydroxybutyrate; back-fat thickness; daily milk production; calving data; lactation number; male parent of the cow; length of gestation; age at first calving; duration of the dry period; fertility parameters of prelactation; diseases during the dry period and up to 3 days postpartum...
November 3, 2017: Tierärztliche Praxis. Ausgabe G, Grosstiere/Nutztiere
https://www.readbyqxmd.com/read/29083435/dairy-intakes-in-older-irish-adults-and-effects-on-vitamin-micronutrient-status-data-from-the-tuda-study
#4
E Laird, M C Casey, M Ward, L Hoey, C F Hughes, K McCarroll, C Cunningham, J J Strain, H McNulty, A M Molloy
BACKGROUND: Consumption of dairy products has been associated with positive health outcomes including a lower risk of hypertension, improved bone health and a reduction in the risk of type 2 diabetes. The suggested dairy intake for health in older adults is three servings per day but recent analysis of the NHANES data for older adults reported 98% were not meeting these recommendations. No studies have investigated the consequences of such declines in the dairy intakes of Irish older adults and the subsequent effects on vitamin micronutrient status...
2017: Journal of Nutrition, Health & Aging
https://www.readbyqxmd.com/read/29050879/one-pot-synthesis-of-gdp-l-fucose-by-a-four-enzyme-cascade-expressed-in-lactococcus-lactis
#5
Ling Li, Seul-Ah Kim, Ji Eun Heo, Tae-Jip Kim, Jin-Ho Seo, Nam Soo Han
GDP-l-fucose is an l-fucose donor to synthesize fucosylated compounds such as human milk oligosaccharides or Lewis antigen. In this study, we used Lactococcus lactis subsp. cremoris NZ9000 to express 4 enzymes, ManB, ManC, Gmd, and WcaG and produced GDP-l-fucose by using one-pot synthesis method with mannose-6-phosphate as substrate and the enzymes as biocatalyst. For preparation of enzyme mixture, 4 genes (manB, manC, gmd, and wcaG) cloned from Escherichia coli were transformed into L. lactis strains using pNZ8008 and the recombinant cell lysates were obtained after cultivation...
October 16, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/29048817/-determination-of-five-nucleotides-in-infant-formula-milk-powder-by-modified-high-performance-liquid-chromatography
#6
Jiong Li, Jiaofei Wang, Hongyu Qiu, Wei Li
A modified high performance liquid chromatographic (HPLC) method was developed for the determination of the five nucleotides (uridine monophosphate (UMP), adenosine monophosphate (AMP), inosine monophosphate (IMP), guanosine monophosphate (GMP) and cytidine monophosphate (CMP)) in infant formula milk powder. The samples were extracted by water, deproteinized by acetic acid and purified with an HLB SPE cartridge. The analytes were separated by a Waters XBrigde Amide column (150 mm×4.6 mm, 3.5 μ m). Acetonitrile, 10 mmol/L sodium dihydrogen phosphate aqueous solution and 0...
August 8, 2017: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/29037495/residuals-of-organophosphate-esters-in-foodstuffs-and-implication-for-human-exposure
#7
Jinjian Ding, Tongqing Deng, Mengmeng Xu, Shen Wang, Fangxing Yang
Foodstuffs may be contaminated by organophosphate esters (OPEs) and become an important source of human exposure since OPEs are ubiquitous in the environment. In the present study, 10 OPEs were analyzed in various food matrices collected from a city in Eastern China including chicken, pork, fishes, vegetables, tofu, eggs, milk and cereals. The concentrations of Σ10OPEs ranged from 1.1 to 9.6 ng g(-1) fresh weight (fw) in the foodstuffs. Cereals had the highest residual level of total OPEs with a mean value of 5...
October 13, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28946300/optimization-of-vitamins-a-and-d3-loading-in-re-assembled-casein-micelles-and-effect-of-loading-on-stability-of-vitamin-d3-during-storage
#8
Anisa Loewen, Benny Chan, Eunice C Y Li-Chan
The objectives of this study were to apply response surface methodology to optimize fat-soluble vitamin loading in re-assembled casein micelles, and to evaluate vitamin D stability of dry formulations during ambient or accelerated storage and in fortified fluid skim milk stored under refrigeration. Optimal loading of vitamin A (1.46-1.48mg/100mgcasein) was found at 9.7mM phosphate, 5.5mM citrate and 30.0mM calcium, while optimal loading of vitamin D (1.38-1.46mg/100mg casein) was found at 4.9mM phosphate, 4...
February 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/28945974/a-combined-aqueous-two-phase-system-and-spot-test-platform-for-the-rapid-detection-of-escherichia-coli-o157-h7-in-milk
#9
Sherine F Cheung, Matthew F Yee, Nguyen K Le, Elizabeth A Gomes, Zahra Afrasiabi, Daniel T Kamei
Foodborne illnesses are a public health concern in the United States and worldwide. Recent outbreaks of Escherichia coli O157:H7 have brought to light the need for improved ways to detect foodborne pathogens and minimize serious outbreaks. Unfortunately, current methods for the detection of foodborne pathogens are time intensive and complex. In this study, we designed a spot immunoassay that uses a UCON-potassium phosphate salt aqueous two-phase system (ATPS) for the preconcentration of O157:H7. This platform was tested with samples of O157:H7 spiked in phosphate-buffered saline and milk...
September 1, 2017: SLAS Technology
https://www.readbyqxmd.com/read/28915420/determination-of-eight-quinolones-in-milk-using-immunoaffinity-microextraction-in-a-packed-syringe-and-liquid-chromatography-with-fluorescence-detection
#10
Xinda Zhang, Cuicui Wang, Linyan Yang, Wei Zhang, Jing Lin, Cun Li
We have established a new, highly selective, and sensitive method for the determination of eight quinolones (QNs) in milk: danofloxacin, enrofloxacin, orbifloxacin, norfloxacin, ofloxacin, lomefloxacin, fleroxacin, and ciprofloxacin. The method uses immunoaffinity microextraction in a packed syringe and liquid chromatography with fluorescence detection (IA-MEPS-LC-FLD). Traditionally, QN residues are determined by liquid-liquid extraction (LLE) and solid phase extraction (SPE) sample preparation techniques; however, these methods are time-consuming and require large quantities of organic solvents...
October 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28912505/biomimetic-silica-nanoparticles-prepared-by-a-combination-of-solid-phase-imprinting-and-ostwald-ripening
#11
Elena Piletska, Heersh Yawer, Francesco Canfarotta, Ewa Moczko, Katarzyna Smolinska-Kempisty, Stanislav S Piletsky, Antonio Guerreiro, Michael J Whitcombe, Sergey A Piletsky
Herein we describe the preparation of molecularly imprinted silica nanoparticles by Ostwald ripening in the presence of molecular templates immobilised on glass beads (the solid-phase). To achieve this, a seed material (12 nm diameter silica nanoparticles) was incubated in phosphate buffer in the presence of the solid-phase. Phosphate ions act as a catalyst in the ripening process which is driven by differences in surface energy between particles of different size, leading to the preferential growth of larger particles...
September 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28888611/comparison-of-mammary-lipid-metabolism-in-dairy-cows-and-goats-fed-diets-supplemented-with-starch-plant-oil-or-fish-oil
#12
L Bernard, P G Toral, Y Chilliard
A direct comparison of cow and goat performance and milk fatty acid (FA) responses to diets known to induce milk fat depression in the bovine has suggested interspecies differences in rumen and mammary lipid metabolism. Thus, this study was conducted to infer some potential mechanisms responsible for the differences in mammary lipogenesis due to diet and ruminant species. To meet this objective, 12 cows and 15 goats were fed a basal diet (control), a similar diet supplemented with 2.2% fish oil (FO), or a diet containing 5...
November 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28880045/-factors-influencing-first-colostrum-calcium-concentration-in-holstein-friesian-cows-and-the-relationship-to-postpartum-calcium-concentration
#13
Maria Karl, Rudolf Staufenbiel
OBJECTIVE: To investigate the factors that affect the calcium content of the first colostrum (milk exclusively of the first withdrawal) and the relationship to the blood calcium concentration. We tested the hypotheses that the lactation number is the main influence factor and that there is a relationship to the postpartum serum calcium concentration. MATERIAL AND METHODS: An observational study was conducted from April 2012 to March 2013 at a commercial dairy farm (2278 Holstein-Friesian cows)...
September 5, 2017: Tierärztliche Praxis. Ausgabe G, Grosstiere/Nutztiere
https://www.readbyqxmd.com/read/28839490/effects-of-prenatal-consumption-of-caprine-milk-oligosaccharides-on-mice-mono-associated-with-bifidobacterium-bifidum-agr2166
#14
Caroline Thum, Kikuji Itoh, Wayne Young, Adrian Cookson, Warren McNabb, Nicole Roy
BACKGROUND: Prenatal consumption of oligosaccharides are associated with changes in the maternal gastrointestinal tract (GIT) microbiota with health consequences for the offspring. It has previously been demonstrated that caprine milk oligosaccharides (CMO) stimulate the growth and fermentation rate of Bifidobacterium bifidum AGR2166. OBJECTIVE: The objective of this study was to examine the effects of B. bifidum AGR2166 and prenatal consumption of CMO, alone or in combination, on the dam's large intestine, foetal development and ability of B...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/28837070/mechanisms-of-antimicrobial-action-of-cinnamon-and-oregano-oils-cinnamaldehyde-carvacrol-2-5-dihydroxybenzaldehyde-and-2-hydroxy-5-methoxybenzaldehyde-against-mycobacterium-avium-subsp-paratuberculosis-map
#15
Stella W Nowotarska, Krzysztof Nowotarski, Irene R Grant, Christopher T Elliott, Mendel Friedman, Chen Situ
The antimicrobial modes of action of six naturally occurring compounds, cinnamon oil, cinnamaldehyde, oregano oil, carvacrol, 2,5-dihydroxybenzaldehyde, and 2-hydroxy-5-methoxybenzaldehyde, previously found to inhibit the growth of Mycobacterium avium subsp. paratuberculosis (Map) reported to infect food animals and humans and to be present in milk, cheese, and meat, were investigated. The incubation of Map cultures in the presence of all six compounds caused phosphate ions to leak into the extracellular environment in a time- and concentration-dependent manner...
August 24, 2017: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28828525/maternal-consumption-of-quinine-containing-sodas-may-induce-g6pd-crises-in-breastfed-children
#16
S Bichali, D Brault, C Masserot, C Boscher, M L Couec, G Deslandes, S Pissard, G Leverger, C Vauzelle, E Elefant, J C Rozé, A Cortey, Alexis Chenouard
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common human enzyme defect often presenting with neonatal jaundice and/or hemolytic anemia. G6PD hemolytic events are linked with exposure to a pro-oxidant agent. We here report three cases of initial G6PD crises in breastfed children secondary to maternal consumption of a tonic drink which contains quinine. Quinine was found in breast milk of one of the mothers after she consumed tonic water. CONCLUSION: The amount of quinine that is transmitted through breast milk appears to be sufficient to induce G6PD crises in breastfed children...
October 2017: European Journal of Pediatrics
https://www.readbyqxmd.com/read/28825541/determination-of-bovine-lactoferrin-in-food-by-hplc-with-a-heparin-affinity-column-for-sample-preparation
#17
Yin Zhang, Fei Lou, Wei Wu, Xin Dong, Jia Ren, Qiuguang Shen
An HPLC method was developed for the quantitative determination of bovine lactoferrin (bLF) in sterilized milk, modified milk, fermented milk, infant formula, adult formula, rice cereal, vitamin function drink, and protein powder products. bLF was first extracted with a phosphate buffer (pH 8), underwent cleanup in a heparin affinity column, and was detected by HPLC with a C4 column and diode-array detector at a wavelength of 280 nm. The proposed method provided a linear detection range of 10.0-1000 μg/mL with an LOD of 0...
January 1, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28756326/bovine-serum-albumin-and-skim-milk-improve-boar-sperm-motility-by-enhancing-energy-metabolism-and-protein-modifications-during-liquid-storage-at-17%C3%A2-%C3%A2-c
#18
Jieli Fu, Yuhua Li, Lirui Wang, Linqing Zhen, Qiangzhen Yang, Peifei Li, Xinhong Li
Both bovine serum albumin (BSA) and skim-milk have been reported to improve sperm quality, primarily by enhancing sperm motility, but the underlying molecular mechanism remains unknown. In this study, boar semen samples were collected and diluted with Androstar(®) Plus extender containing different concentrations (0, 2, 4 g/l) of BSA and skim-milk. On days 0, 3, 5 and 7, the sperm motility parameters were determined using computer-assisted sperm analysis (CASA), and the ATP concentrations, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity and mitochondrial membrane potential were evaluated using commercial kits...
July 21, 2017: Theriogenology
https://www.readbyqxmd.com/read/28747832/effects-of-various-calcium-powders-as-replacers-for-synthetic-phosphate-on-the-quality-properties-of-ground-pork-meat-products
#19
Su Min Bae, Min Guk Cho, Jong Youn Jeong
The aim of this study was to identify the optimal and superior type of natural calcium for replacing phosphate in cooked ground pork products. To achieve this, 0.5% eggshell calcium (ESC), oyster shell calcium (OSC), marine algae calcium (MAC), or milk calcium (MC) was added to ground pork meat products. The effect of this substitution was studied by comparing the substituted products with products containing 0.3% phosphate blend (control). ESC was considered an ideal phosphate replacer for minimizing the cooking loss, which likely resulted from the increase in the pH of the product...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/28712055/potential-of-casein-as-a-carrier-for-biologically-active-agents
#20
REVIEW
Tomasz Konrad Głąb, Janusz Boratyński
Casein is the collective name for a family of milk proteins. In bovine milk, casein comprises four peptides: αS1, αS2, β, and κ, differing in their amino acid, phosphorus and carbohydrate content but similar in their amphiphilic character. Hydrophilic and hydrophobic regions of casein show block distribution in the protein chain. Casein peptides carry negative charge on their surface as a result of phosphorylation and tend to bind nanoclusters of amorphous calcium phosphate. Due to these properties, in suitable conditions, casein molecules agglomerate into spherical micelles...
August 2017: Topics in Current Chemistry (Journal)
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