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Milk phosphate

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https://www.readbyqxmd.com/read/28434752/effects-of-intrauterine-infusion-of-escherichia-coli-lipopolysaccharide-on-uterine-health-resolution-of-purulent-vaginal-discharge-and-reproductive-performance-of-lactating-dairy-cows
#1
João G N Moraes, Paula R B Silva, Luís G D Mendonça, Alexandre A Scanavez, Joseane C C Silva, Ricardo C Chebel
The objectives of the current experiment were to evaluate the effects of intrauterine infusion of Escherichia coli lipopolysaccharide (LPS) in cows diagnosed with purulent vaginal discharge (PVD) on intrauterine cell population, resolution of PVD, uterine health, and reproductive performance. Jersey cows (n = 3,084) were examined using the Metricheck device to diagnose PVD at 35 ± 6 d postpartum. Purulent vaginal discharge was defined as the presence of purulent (≥50% pus) discharge detectable in the vagina...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28434743/the-effect-of-emulsifying-salts-on-the-turbidity-of-a-diluted-milk-system-with-varying-ph-and-protein-concentration
#2
M D Culler, Y Saricay, F M Harte
Solutions of 10 commonly used emulsifying salts (ES) listed in the Code of Federal Regulations (21CFR133.179) for pasteurized process cheese were tested for their effect on the turbidity of a diluted milk system at different pH and protein concentrations to characterize the conditions that affect micellar structure. Emulsifying salt solutions were made by mixing the ES in a 1-in-20 dilution of water in skim milk ultrafiltrate (3 kDa molecular weight cut-off) to obtain ES concentrations from 0 to 248 mM. Skim milk was added to solutions containing nanopure water, skim milk ultrafiltrate, and a specific ES ranging in concentration from 0 to 248 mM and pH 5, 5...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28434729/effects-of-intrauterine-infusion-of-escherichia-coli-lipopolysaccharide-on-uterine-mrna-gene-expression-and-peripheral-polymorphonuclear-leukocytes-in-jersey-cows-diagnosed-with-purulent-vaginal-discharge
#3
João G N Moraes, Luís G D Mendonça, Paula R B Silva, Alexandre A Scanavez, Klibs N Galvão, Michael A Ballou, Milli Worku, Ricardo C Chebel
The objectives of the current experiment were to investigate the effects of intrauterine treatment of cows with purulent vaginal discharge (PVD) using lipopolysaccharide (LPS) from Escherichia coli on uterine mRNA expression of genes related to inflammatory responses, peripheral polymorphonuclear leukocyte (PMN) function, hematological parameters, and blood concentrations of cortisol, haptoglobin, and progesterone (P4). Jersey cows (n = 3,084) were examined for PVD at 35 ± 6 d postpartum using the Metricheck device (Simcro, Hamilton, New Zealand)...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28420530/rapid-determination-of-the-various-native-forms-of-vitamin-b6-and-b2-in-cow-s-milk-using-ultra-high-performance-liquid-chromatography
#4
A Schmidt, M G Schreiner, H K Mayer
As the formation of pyridoxal phosphate, the active cofactor of vitamin B6, is dependent on riboflavin 5-phosphate, we propose a fast and simple ultra-high performance liquid chromatography method for the simultaneous determination of the native B6 vitamers pyridoxal, pyridoxine, pyridoxamine, their mono phosphorus esters and 4-pyridoxic acid as well as vitamin B2 as riboflavin and its phosphorus ester riboflavin 5-phosphate in milk. Separation was achieved under 6.0min by reversed-phase and pH gradient elution...
April 9, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28403179/new-route-for-self-assembly-of-%C3%AE-lactalbumin-nanotubes-and-their-use-as-templates-to-grow-silver-nanotubes
#5
Wei-Chun Fu, Mauricio A Opazo, Sergio M Acuña, Pedro G Toledo
Nanotubes are formed by self-assembly of α-lactalbumin milk protein following a different route than established for the hydrolysis which involves V8 enzyme, phosphate buffer and appropriate amounts of calcium at neutral pH. The resulting nanotubes are used as templates for the growth of conductive silver nanotubes. TEM, SEM-EDS, AFM and FTIR are used for characterization.
2017: PloS One
https://www.readbyqxmd.com/read/28390667/pyridoxal-5-phosphate-mediated-preparation-of-immobilized-metal-affinity-material-for-highly-selective-and-sensitive-enrichment-of-phosphopeptides
#6
Qian Wang, Xiao-Mei He, Xi Chen, Gang-Tian Zhu, Ren-Qi Wang, Yu-Qi Feng
Phosphorylation is a crucial post-translational modification, which plays pivotal roles in various biological processes. Analysis of phosphopeptides by mass spectrometry (MS) is intractable on account of their low stoichiometry and the ion suppression from non-phosphopeptides. Thus, enrichment of phosphopeptides before MS analysis is indispensable. In this work, we employed pyridoxal 5'-phosphate (PLP), as an immobilized metal affinity chromatography (IMAC) ligand for the enrichment of phosphopeptides. PLP was grafted onto several substrates such as silica (SiO2), oxidized carbon nanotube (OCNT) and silica coated magnetic nanoparticles (Fe3O4@SiO2)...
April 1, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28342973/effects-of-a-sustained-release-formulation-of-1-25-dihydroxyvitamin-d3-glycosides-for-milk-fever-prevention-on-serum-1-25-dihydroxyvitamin-d3-calcium-and-phosphorus-in-dairy-cows
#7
Heinrich Bachmann, Michael Lanz, Susanne Kehrle, Wolfgang Bittner, Annick Toggenburger, Georg A Mathis, Walter Rambeck
Milk fever (MF) is a metabolic disease in dairy cows around parturition. The clinical lead sign is muscular paresis leading in severe cases to paralysis of the affected animal. Multiparturient animals of high performing dairy breeds are most likely to be affected and have a high probability of recurrence. An acute drop in blood calcium levels causes the disease when the demand for calcium at the onset of lactation exceeds the ability to replete blood calcium levels through mobilization from bone and intestinal uptake...
March 23, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28342601/ultra-performance-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry-ms-e-based-untargeted-milk-metabolomics-in-dairy-cows-with-subclinical-or-clinical-mastitis
#8
Xiaomin Xi, Lai-Yu Kwok, Yuenan Wang, Chen Ma, Zhihui Mi, Heping Zhang
In this study, a novel metabolomics technique based on ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry in the MS(E) mode was used to investigate the milk metabolomics of healthy, subclinical, and clinical mastitis cows, which were classified based on somatic cell count and presentation of clinical symptoms. Meanwhile, univariate and multivariate statistical analyses were performed to identify the significant differences across the 3 groups. Compared with healthy milk samples, less glucose, d-glycerol-1-phosphate, 4-hydroxyphenyllactate, l-carnitine, sn-glycero-3-phosphocholine, citrate, and hippurate were detected in the clinical mastitic milk samples, whereas less d-glycerol-1-phosphate, benzoic acid, l-carnitine, and cis-aconitate were found in the subclinical mastitic milk samples...
March 22, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28316482/monitoring-of-bioluminescent-lactobacillus-plantarum-in-a-complex-food-matrix
#9
Gi-Seong Moon, Arjan Narbad
A bioluminescent Lactobacillus plantarum (pLuc2) strain was constructed. The luminescent signal started to increase during the early exponential phase and reached its maximum in the mid-exponential phase in a batch culture of the strain. The signal detection sensitivity of the strain was the highest in PBS (phosphate buffered saline), followed by milk and MRS broth, indicating that the sensitivity was influenced by the matrix effect. The strain was used in millet seed fermentation which has a complex matrix and native lactic acid bacteria (LAB)...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/28303318/bone-alkaline-phosphatase-and-tartrate-resistant-acid-phosphatase-potential-co-regulators-of-bone-mineralization
#10
Cecilia Halling Linder, Barbro Ek-Rylander, Michael Krumpel, Maria Norgård, Sonoko Narisawa, José Luis Millán, Göran Andersson, Per Magnusson
Phosphorylated osteopontin (OPN) inhibits hydroxyapatite crystal formation and growth, and bone alkaline phosphatase (BALP) promotes extracellular mineralization via the release of inorganic phosphate from the mineralization inhibitor inorganic pyrophosphate (PPi). Tartrate-resistant acid phosphatase (TRAP), produced by osteoclasts, osteoblasts, and osteocytes, exhibits potent phosphatase activity towards OPN; however, its potential capacity as a regulator of mineralization has not previously been addressed...
March 16, 2017: Calcified Tissue International
https://www.readbyqxmd.com/read/28290293/comparative-proteomic-analysis-of-milk-derived-exosomes-in-human-and-bovine-colostrum-and-mature-milk-samples-by-itraq-coupled-lc-ms-ms
#11
Mei Yang, Dahe Song, Xueyan Cao, Rina Wu, Biao Liu, Wenhui Ye, Junrui Wu, Xiqing Yue
Exosomes are membranous vesicles found in biological fluids with important functions. However, milk-derived exosome proteins from humans and bovines have not been studied in detail. The advanced iTRAQ proteomic approach was used to analyze milk-derived exosomes in human and bovine colostrum and mature milk samples. A total of 920 milk exosome proteins were identified and quantified. Among these, 575 differentially expressed exosome proteins (P<0.05) were found. Multivariate analysis, gene ontology (GO) annotation and the KEGG pathway were used to interpret the identified proteins...
February 2017: Food Research International
https://www.readbyqxmd.com/read/28281038/effect-of-intramammary-infusion-of-recombinant-bovine-gm-csf-and-il-8-on-cmt-score-somatic-cell-count-and-milk-mononuclear-cell-populations-in-holstein-cows-with-staphylococcus-aureus-subclinical-mastitis
#12
Yoshio Kiku, Tomomi Ozawa, Hideyuki Takahashi, Shiro Kushibiki, Shigeki Inumaru, Hiroyuki Shingu, Yuya Nagasawa, Atsushi Watanabe, Eiji Hata, Tomohito Hayashi
The effect of intramammary infusion of recombinant bovine granulocyte-macrophage colony-stimulating factor (rbGM-CSF) and interleukin-8 (rbIL-8) on mononuclear cell populations in quarters, somatic cell count (SCC) and the California Mastitis Test (CMT) score were investigated. From the selected cows with naturally occurring Staphylococcus aureus subclinical mastitis, one quarter of each cow were selected for the infusions of rbGM-CSF (400 μg/5 mL/quarter, n = 9), rbIL-8 (1 mg/5 mL/quarter, n = 9), and phosphate-buffered saline (5 mL/quarter, n = 7)...
March 9, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28242943/rheological-and-physical-properties-of-camel-and-cow-milk-gels-enriched-with-phosphate-and-calcium-during-acid-induced-gelation
#13
Mohammad Kamal, Mohammed Foukani, Romdhane Karoui
The rheological properties of acid-induced coagulation of camel and cow milk gels following the addition of calcium chloride (CaCl2) and hydrogen phosphate dehydrate (Na2HPO4*2H2O) were investigated using a dynamic low amplitude oscillatory rheology. For a considered condition, the final values of storage modulus (G') and loss modulus (G″) of camel milk gels were significantly lower than those of cow milk gels. The increase of the added CaCl2 levels improved significantly the gelation properties of camel and cow milk gels, since a reduction in the gelation time and an increase in the gel firmness were observed...
February 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28221844/evaluation-of-commercial-milk-specific-lateral-flow-devices
#14
Rachel C Courtney, Steve L Taylor, Joe L Baumert
The requirement for validation of allergen cleaning processes is increasing. The use of lateral flow devices (LFDs) to identify allergens has rapidly expanded, but the best practices for use of these devices are still developing. The goal of this study was to compare commercially available milk-specific LFDs and a general protein identification method. Five milk proteins and seven milk-derived ingredients were tested at several concentrations with eight milk-specific LFDs and a general protein identification kit...
October 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28213246/spinel-type-manganese-ferrite-mnfe2o4-microspheres-a-novel-affinity-probe-for-selective-and-fast-enrichment-of-phosphopeptides
#15
Xing-Yu Long, Jia-Yuan Li, Dong Sheng, Hong-Zhen Lian
The spinel-type magnetic manganese ferrite (MnFe2O4) microspheres synthesized by simple solvothermal method were used as a novel adsorbent for selective enrichment and effective isolation of phosphopeptides. The uniform MnFe2O4 magnetic affinity microspheres (MAMSs) had a narrow particle size distribution between 250 and 260nm, and displayed superparamagnetism with a saturation magnetization value of 67.0emu/g. Comprehensively, the possible formation mechanism of MnFe2O4 microspheres with ferric and manganous sources as dual precursors was elucidated by comparison with those of Fe3O4 nanoparticles and MnOOH nanosheets respectively with either ferric or manganous source as single precursor...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28192612/advantage-of-eu-3-doped-polystyrene-microspheres-compared-with-colloidal-gold-used-in-immunochromatographic-assays-for-the-detection-of-melamine-in-milk
#16
Yiwei Tang, Hong Zhang, Xiuying Liu, Evan Trofimchuk, Shaolong Feng, Tao Ma, Xue Gao, Shubing Fang, Xiaonan Lu
Colloidal gold and Eu(3+) -doped fluorescent microspheres were applied as labels to develop the immunochromatographic strips for detecting melamine in milk. Under the optimized condition, the visual detection limit of colloidal gold-immunochromatographic test strip (ICTS) was 150 μg/L of melamine in phosphate-buffered saline (PBS), although the visual detection limit of fluorescent nanoparticles (FN)-ICTS was 75 μg/L in PBS. As thermal acceleration test, FN-ICTS could be stored at 37 °C for at least 11 d before sample testing, but the color of the lines on colloidal gold-ICTS faded away after 7-d storage...
February 13, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28188640/rennet-induced-coagulation-of-raw-and-heated-camel-and-cow-milk-gels-determined-by-instrumental-techniques-effects-of-added-calcium-and-phosphate
#17
Romdhane Karoui, Mohammad Kamal
BACKGROUND: The potentiality of the front-face fluorescence spectroscopy and rheological measurements combined with chemometric tools to characterize the structure evolution during coagulation of raw and preheated camel and cow milk at 50 and 70 °C with/without added calcium and phosphate was evaluated. Tryptophan and vitamin A fluorescence spectra were collected during the gelation of milk at 0, 10, 20, 30, 40, and 45 min after the addition of rennet-induced coagulation. In parallel, the storage modulus (G'), loss modulus (G") and tan delta (tan δ) were determined using low amplitude oscillation shear analysis...
February 11, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28173647/-analysis-of-newborn-screening-for-galactosemia-and-genotype-phenotype-of-confirmed-galatosemia-cases
#18
R L Yang, F Tong, F Hong, G L Qian, D W Wu, Z Y Zhao
Objective: To investigate the prevalence of galactosemia(GAL), and the characteristics of genotype and phenotype of newborns who were confirmed with GAL in newborn screening in Zhejiang province. Method: The number of all live births, newborn screened infants and all clinical data of confirmed newborns with GAL from October 2013 to March 2015 were retrospectively analyzed by reviewing the data of Zhejiang Province screening center database. And the characteristics of genes and the clinical data of GAL cases who were confirmed by correlative gene test and enzyme activity measurement were analyzed...
February 2, 2017: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
https://www.readbyqxmd.com/read/28166927/effect-of-artificial-rearing-of-piglets-on-the-volume-densities-of-m-cells-in-the-tonsils-of-the-soft-palate-and-ileal-peyer-s-patches
#19
S Prims, N Pintens, H Vergauwen, S Van Cruchten, C Van Ginneken, C Casteleyn
The high prolificacy of modern hybrid sows has increased the mean litter size during the last decades. However, rearing large litters is challenging and has increased the use of alternative management strategies such as euthanasia of weak piglets, cross-fostering, supplementing piglets with milk, split-nursing and split-weaning. The latter includes artificial rearing on brooders where piglets have ad libitum access to milk replacer. The effect of this artificial rearing on the immune system of the piglet is the subject of various studies...
February 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28127877/facile-synthesis-of-ti-4-immobilized-affinity-silica-nanoparticles-for-the-highly-selective-enrichment-of-intact-phosphoproteins
#20
Yanting He, Wei Liu, Lei Chen, Guo Lin, Qi Xiao, Chenling Gao, Wu Jianlin, Zian Lin
Currently, great challenges to top-down phosphoproteomics lie in the selective enrichment of intact phosphoproteins from complex biological samples. Herein, we developed a facile approach for synthesis of Ti(4+) -immobilized affinity silica nanoparticles and applied them to the selective separation and enrichment of intact phosphoproteins based upon the principle of metal(IV) phosphate/phosphonate chemistry. The as-prepared affinity materials exhibited high selectivity and adsorption capacities for model phosphoproteins (328...
January 27, 2017: Journal of Separation Science
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