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Milk phosphate

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https://www.readbyqxmd.com/read/29329924/effects-of-dark-chocolate-on-endothelial-function-in-patients-with-non-alcoholic-steatohepatitis
#1
L Loffredo, F Baratta, P Ludovica, S Battaglia, R Carnevale, C Nocella, M Novo, G Pannitteri, F Ceci, F Angelico, F Violi, M Del Ben
BACKGROUND AND AIM: Oxidative stress plays a pivotal role in inducing endothelial dysfunction and progression from simple fatty liver steatosis (FLD) to non-alcoholic steatohepatitis (NASH). Polyphenols could reduce oxidative stress and restore endothelial function by inhibiting the nicotinamide-adenine-dinucleotide-phosphate (NADPH) oxidase isoform Nox2. The aim of this study was to assess endothelial function and oxidative stress in a population affected by simple FLD and NASH. Furthermore, we analysed the effect of high vs low content of cocoa polyphenols on endothelial function and oxidative stress in patients with NASH...
November 13, 2017: Nutrition, Metabolism, and Cardiovascular Diseases: NMCD
https://www.readbyqxmd.com/read/29274129/newborn-screening-for-galactosaemia
#2
REVIEW
Rohollah Lak, Bahareh Yazdizadeh, Majid Davari, Mojtaba Nouhi, Roya Kelishadi
BACKGROUND: Classical galactosaemia is an autosomal recessive inborn error of metabolism caused by a deficiency of the enzyme galactose-1-phosphate uridyltransferase. This is a rare and potentially lethal condition that classically presents in the first week of life once milk feeds have commenced. Affected babies may present with any or all of the following: cataracts; fulminant liver failure; prolonged jaundice; or Escherichia coli sepsis. Once the diagnosis is suspected, feeds containing galactose must be stopped immediately and replaced with a soya-based formula...
December 23, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/29202723/changes-in-urinary-risk-profile-after-short-term-low-sodium-and-low-calcium-diet-in-recurrent-swiss-kidney-stone-formers
#3
Harald Seeger, Andrea Kaelin, Pietro M Ferraro, Damian Weber, Philippe Jaeger, Patrice Ambuehl, William G Robertson, Robert Unwin, Carsten A Wagner, Nilufar Mohebbi
BACKGROUND: Kidney stone disease is common in industrialized countries. Recently, it has attracted growing attention, because of its significant association with adverse renal outcomes, including end stage renal disease. Calcium-containing kidney stones are frequent with high recurrence rates. While hypercalciuria is a well-known risk factor, restricted intake of animal protein and sodium, combined with normal dietary calcium, has been shown to be more effective in stone prevention compared with a low-calcium diet...
December 4, 2017: BMC Nephrology
https://www.readbyqxmd.com/read/29195966/thermal-stability-of-reconstituted-milk-protein-concentrates-effect-of-partial-calcium-depletion-during-membrane-filtration
#4
H Eshpari, R Jimenez-Flores, P S Tong, M Corredig
Milk protein concentrate (MPC) powders are increasingly utilized in manufacturing of protein fortified beverages. Thermal stability of the protein dispersions is of significant importance in such applications. It is known that a decrease in pH can induce partial dissociation of casein micelles and modify the natural equilibrium of calcium and phosphate between the micelles and the serum phase. The presence of soluble casein may improve the rehydration properties of MPC powders, and may impact their thermal stability...
December 2017: Food Research International
https://www.readbyqxmd.com/read/29195946/rennet-induced-coagulation-properties-of-yak-casein-micelles-a-comparison-with-cow-casein-micelles
#5
Yan Zhang, Yuan Li, Pengjie Wang, Yanbao Tian, Qi Liang, Fazheng Ren
It is essential for yak cheese processing to understand the rennet-induced coagulation properties of gel formation from casein micelles. We have previously discovered that yak milk requires a longer incubation time but forms stronger gels compared with cow milk. In this study, we are aiming to understand the rennet-induced coagulation properties of yak casein micelles comparing with cow casein micelles. Rheological analyses revealed that the gelling times of yak and cow casein micelles were 11.6±0.5 and 8...
December 2017: Food Research International
https://www.readbyqxmd.com/read/29195135/urinary-metabolites-of-organophosphate-esters-concentrations-and-age-trends-in-australian-children
#6
Chang He, Leisa-Maree L Toms, Phong Thai, Nele Van den Eede, Xianyu Wang, Yan Li, Christine Baduel, Fiona A Harden, Amy L Heffernan, Peter Hobson, Adrian Covaci, Jochen F Mueller
There is growing concern around the use of organophosphate esters (OPEs) due to their suspected reproductive toxicity, carcinogenicity, and neurotoxicity. OPEs are used as flame retardants and plasticizers, and due to their extensive application in consumer products, are found globally in the indoor environment. Early life exposure to OPEs is an important risk factor for children's health, but poorly understood. To study age and sex trends of OPE exposures in infants and young children, we collected, pooled, and analysed urine samples from children aged 0-5years from Queensland, Australia for 9 parent OPEs and 11 metabolites...
November 28, 2017: Environment International
https://www.readbyqxmd.com/read/29183573/age-dependent-physiological-dynamics-in-acid-base-balance-electrolytes-and-blood-metabolites-in-growing-goats
#7
S Redlberger, S Fischer, H Köhler, R Diller, P Reinhold
There is a paucity of published data reporting acid-base equilibrium in goats, and no information is available on how the acid-base complexity changes when suckling goat kids become ruminants. The aims of this study were to evaluate young healthy goats for age-related changes in serum proteins, metabolites, and electrolytes; differences in results when the Henderson-Hasselbalch equation or strong ion approaches were used were also assessed. To assess biological variability and reproducibility, two consecutive long-term studies, each lasting from the 6th to 56th week of life (wl), were performed in 15 (Study 1) and 10 (Study 2) animals...
November 2017: Veterinary Journal
https://www.readbyqxmd.com/read/29182545/detection-of-abrin-holotoxin-using-novel-monoclonal-antibodies
#8
Xiaohua He, Stephanie Patfield, Luisa W Cheng, Larry H Stanker, Reuven Rasooly, Thomas A McKeon, Yuzhu Zhang, David L Brandon
Abrin, a member of the ribosome-inactivating protein family, is produced by the Abrus precatorius plant. Having the potential to pose a severe threat to both human and animal health, abrin is classified as a Select Agent by the U.S. Department of Health and Human Services. However, an immunoassay that is specific for intact abrin holotoxin has not yet been reported. In this study, seven new monoclonal antibodies (mAbs), designated as Abrin-1 through Abrin-7 have been developed. Isotyping analyses indicate these mAbs have IgG1, IgG2a, or IgG2b heavy-chains and kappa light-chains...
November 28, 2017: Toxins
https://www.readbyqxmd.com/read/29175366/lack-of-the-nad-dependent-glycerol-3-phosphate-dehydrogenase-impairs-the-function-of-transcription-factors-sip4-and-cat8-required-for-ethanol-utilization-in-kluyveromyces-lactis
#9
Lucía Mojardín, Montserrat Vega, Fernando Moreno, Hans-Peter Schmitz, Jürgen J Heinisch, Rosaura Rodicio
The NAD+-dependent glycerol 3-phosphate dehydrogenase (KlGpd1) is an important enzyme for maintenance of the cytosolic redox balance. In the milk yeast Kluyveromyces lactis The enzyme is localized in peroxisomes and in the cytosol, indicating its requirement for the oxidation of NADH in both compartments. Klgpd1 mutants grow more slowly on glucose than wild-type cells and do not grow on ethanol as a sole carbon source. We studied the molecular basis of the latter phenotype and found that Gpd1 is required for high expression of KlICL1 and KlMLS1 which encode the key enzymes of the glyoxylate pathway isocitrate lyase and malate synthase, respectively...
November 22, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/29158139/expression-and-evaluation-of-recombinant-p32-protein-based-elisa-for-sero-diagnostic-potential-of-capripox-in-sheep-and-goats
#10
Gnanavel Venkatesan, Mahesh Kumar Teli, Muthu Sankar, Amit Kumar, M Dashprakash, Sargam Arya, Aparna Madhavan, Muthannan Andavar Ramakrisnan, Awadh Bihari Pandey
The study is aimed to develop and evaluate a recombinant P32 protein based ELISA for sero-monitoring and sero-surveillance using known and suspected serum samples for capripox from sheep and goats. Truncated P32 gene of goatpox virus (with an ORF of 750 bp) was expressed in E. coli BL-21 CodonPlus (DE3) cells using pET32a vector and characterized by SDS-PAGE analysis and confirmed by western blotting as 48 KDa Poly-His tagged fusion protein. The protein was purified under denaturing conditions using 8M urea and characterized by SDS-PAGE and immunoblotting...
November 17, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/29153180/a-100-year-review-progress-on-the-chemistry-of-milk-and-its-components
#11
John A Lucey, Don Otter, David S Horne
Understanding the chemistry of milk and its components is critical to the production of consistent, high-quality dairy products as well as the development of new dairy ingredients. Over the past 100 yr we have gone from believing that milk has only 3 protein fractions to identifying all the major and minor types of milk proteins as well as discovering that they have genetic variants. The structure and physical properties of most of the milk proteins have been extensively studied. The structure of the casein micelle has been the subject of many studies, and the initial views on submicelles have given way to the current model of the micelle as being assembled as a result of the concerted action of several types of interactions (including hydrophobic and the formation of calcium phosphate nanoclusters)...
December 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29136806/multivariate-analysis-of-organic-acids-in-fermented-food-from-reversed-phase-high-performance-liquid-chromatography-data
#12
Pablo Mortera, Federico A Zuljan, Christian Magni, Santiago A Bortolato, Sergio H Alarcón
Multivariate calibration coupled to RP-HPLC with diode array detection (HPLC-DAD) was applied to the identification and the quantitative evaluation of the short chain organic acids (malic, oxalic, formic, lactic, acetic, citric, pyruvic, succinic, tartaric, propionic and α-cetoglutaric) in fermented food. The goal of the present study was to get the successful resolution of a system in the combined occurrence of strongly coeluting peaks, of distortions in the time sensors among chromatograms, and of the presence of unexpected compounds not included in the calibration step...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29136753/-metabolomics-study-of-tris-2-chloroethyl-phosphate-induced-hepaotoxicity-and-nephrotoxicity-in-sprague-dawley-rats
#13
W Q Yang, F Zhao, L Li, Y J Fang
Objective: To discuss the potential toxic target organ and the toxic effects and mechanisms of tris (2-chloroethyl) phosphate (TCEP) on SD rats. Methods: 40 female SD rats weaning from milk for 21 days, weighted (50±2.3)g were selected as subjects and marked by the weight. They were randomly divided into 4 groups, namely control group, 50 (L), 100 (M) and 250 (H) mg·kg(-1)·d(-1) dose of TCEP group. Each group has 10 rats, and administrated the corresponding dose of drug or vehicle by mouth, quaque die for 60 days...
November 6, 2017: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/29099901/-factors-influencing-the-quality-of-the-first-colostrum-in-holstein-friesian-cows-and-their-relationship-to-postpartum-calcium-concentrations-in-the-blood-and-colostrum
#14
Maria Karl, Rudolf Staufenbiel
OBJECTIVE: To investigate the factors that affect the quality (immunoglobulin concentration) of the first colostrum in Holstein Friesian cows and their relationship to the calcium concentrations in the blood and colostrum, with particular attention to the lactation number. MATERIAL AND METHODS: An observational study was conducted at a commercial dairy farm (2278 Holstein Friesian cows). The analysis comprised the following variables: colostrum content of immunoglobulins and calcium, colostrum yield; blood serum concentrations of calcium, phosphate, non-esterified fatty acids, and β-hydroxybutyrate; back-fat thickness; daily milk production; calving data; lactation number; male parent of the cow; length of gestation; age at first calving; duration of the dry period; fertility parameters of prelactation; diseases during the dry period and up to 3 days postpartum...
November 3, 2017: Tierärztliche Praxis. Ausgabe G, Grosstiere/Nutztiere
https://www.readbyqxmd.com/read/29083435/dairy-intakes-in-older-irish-adults-and-effects-on-vitamin-micronutrient-status-data-from-the-tuda-study
#15
E Laird, M C Casey, M Ward, L Hoey, C F Hughes, K McCarroll, C Cunningham, J J Strain, H McNulty, A M Molloy
BACKGROUND: Consumption of dairy products has been associated with positive health outcomes including a lower risk of hypertension, improved bone health and a reduction in the risk of type 2 diabetes. The suggested dairy intake for health in older adults is three servings per day but recent analysis of the NHANES data for older adults reported 98% were not meeting these recommendations. No studies have investigated the consequences of such declines in the dairy intakes of Irish older adults and the subsequent effects on vitamin micronutrient status...
2017: Journal of Nutrition, Health & Aging
https://www.readbyqxmd.com/read/29050879/one-pot-synthesis-of-gdp-l-fucose-by-a-four-enzyme-cascade-expressed-in-lactococcus-lactis
#16
Ling Li, Seul-Ah Kim, Ji Eun Heo, Tae-Jip Kim, Jin-Ho Seo, Nam Soo Han
GDP-l-fucose is an l-fucose donor to synthesize fucosylated compounds such as human milk oligosaccharides or Lewis antigen. In this study, we used Lactococcus lactis subsp. cremoris NZ9000 to express 4 enzymes, ManB, ManC, Gmd, and WcaG and produced GDP-l-fucose by using one-pot synthesis method with mannose-6-phosphate as substrate and the enzymes as biocatalyst. For preparation of enzyme mixture, 4 genes (manB, manC, gmd, and wcaG) cloned from Escherichia coli were transformed into L. lactis strains using pNZ8008 and the recombinant cell lysates were obtained after cultivation...
October 16, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/29048817/-determination-of-five-nucleotides-in-infant-formula-milk-powder-by-modified-high-performance-liquid-chromatography
#17
Jiong Li, Jiaofei Wang, Hongyu Qiu, Wei Li
A modified high performance liquid chromatographic (HPLC) method was developed for the determination of the five nucleotides (uridine monophosphate (UMP), adenosine monophosphate (AMP), inosine monophosphate (IMP), guanosine monophosphate (GMP) and cytidine monophosphate (CMP)) in infant formula milk powder. The samples were extracted by water, deproteinized by acetic acid and purified with an HLB SPE cartridge. The analytes were separated by a Waters XBrigde Amide column (150 mm×4.6 mm, 3.5 μ m). Acetonitrile, 10 mmol/L sodium dihydrogen phosphate aqueous solution and 0...
August 8, 2017: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/29037495/residuals-of-organophosphate-esters-in-foodstuffs-and-implication-for-human-exposure
#18
Jinjian Ding, Tongqing Deng, Mengmeng Xu, Shen Wang, Fangxing Yang
Foodstuffs may be contaminated by organophosphate esters (OPEs) and become an important source of human exposure since OPEs are ubiquitous in the environment. In the present study, 10 OPEs were analyzed in various food matrices collected from a city in Eastern China including chicken, pork, fishes, vegetables, tofu, eggs, milk and cereals. The concentrations of Σ10OPEs ranged from 1.1 to 9.6 ng g(-1) fresh weight (fw) in the foodstuffs. Cereals had the highest residual level of total OPEs with a mean value of 5...
October 13, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28946300/optimization-of-vitamins-a-and-d3-loading-in-re-assembled-casein-micelles-and-effect-of-loading-on-stability-of-vitamin-d3-during-storage
#19
Anisa Loewen, Benny Chan, Eunice C Y Li-Chan
The objectives of this study were to apply response surface methodology to optimize fat-soluble vitamin loading in re-assembled casein micelles, and to evaluate vitamin D stability of dry formulations during ambient or accelerated storage and in fortified fluid skim milk stored under refrigeration. Optimal loading of vitamin A (1.46-1.48mg/100mgcasein) was found at 9.7mM phosphate, 5.5mM citrate and 30.0mM calcium, while optimal loading of vitamin D (1.38-1.46mg/100mg casein) was found at 4.9mM phosphate, 4...
February 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/28945974/a-combined-aqueous-two-phase-system-and-spot-test-platform-for-the-rapid-detection-of-escherichia-coli-o157-h7-in-milk
#20
Sherine F Cheung, Matthew F Yee, Nguyen K Le, Elizabeth A Gomes, Zahra Afrasiabi, Daniel T Kamei
Foodborne illnesses are a public health concern in the United States and worldwide. Recent outbreaks of Escherichia coli O157:H7 have brought to light the need for improved ways to detect foodborne pathogens and minimize serious outbreaks. Unfortunately, current methods for the detection of foodborne pathogens are time intensive and complex. In this study, we designed a spot immunoassay that uses a UCON-potassium phosphate salt aqueous two-phase system (ATPS) for the preconcentration of O157:H7. This platform was tested with samples of O157:H7 spiked in phosphate-buffered saline and milk...
September 1, 2017: SLAS Technology
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