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https://www.readbyqxmd.com/read/28087055/graft-modification-of-cotton-with-phosphate-group-and-its-application-to-the-enrichment-of-phosphopeptides
#1
Xiao-Mei He, Xi Chen, Bi-Feng Yuan, Yu-Qi Feng
Grafting copolymerization, especially "grafting from" approach, has attracted a great attention for the preparation of cellulose-based materials with various functionalities. In this study, a novel phosphate group-containing cotton fiber-based material, CF-NH2-AZO-p(VPA-x), was successfully synthesized by a "grafting from" radical polymerization approach using vinyl phosphonic acid (VPA) as monomer. The phosphate group content of CF-NH2-AZO-p(VPA-x) could be easily regulated by adjusting the concentration of VPA monomer...
January 9, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28081252/corn-soy-blend-fortified-with-phosphorus-to-prevent-refeeding-hypophosphatemia-in-undernourished-piglets
#2
Anne-Louise Hother, Mikkel Lykke, Torben Martinussen, Hanne Damgaard Poulsen, Christian Mølgaard, Per Torp Sangild, André Briend, Christian Fink Hansen, Henrik Friis, Kim F Michaelsen, Thomas Thymann
BACKGROUND: Phosphorus (P) levels in refeeding diets are very important as undernourished children are at risk of hypophosphatemia during refeeding. For this reason, conventional corn-soy-blends (CSB) have been reformulated by the World Food Programme to obtain a mono-calcium-phosphate fortified product (CSB+) and a product further fortified with skim milk powder (CBS++). METHODS: Using a piglet model of undernourished children, we hypothesized that feeding of CSB+, CSB++ or CSB+ with added whey permeate (CSB+/wp) would help to prevent refeeding hypophosphatemia...
2017: PloS One
https://www.readbyqxmd.com/read/28043636/evaluation-the-virulence-of-mycobacterium-bovis-isolated-from-milk-samples-through-histopathological-study-in-laboratory-animals
#3
I M Al-Saqur, A N Al-Thwani, I M Al-Attar, M S Al-Mashhadani
INTRODUCTION: Mycobacterium bovis has a broad host range, and it is the principal agent responsible for tuberculosis (TB) in bovine, domestic and wild mammals. M. bovis also infects human, causing zoonotic TB through ingestion, inhalation and, less frequently by contact with mucous membranes and broken skin. Zoonotic TB was formerly an endemic disease, usually transmitted to man by consumption of raw cow's milk. It is indistinguishable clinically or pathologically from TB caused by M...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28040178/blueberry-proanthocyanidins-against-human-norovirus-surrogates-in-model-foods-and-under-simulated-gastric-conditions
#4
Snehal Joshi, Amy B Howell, Doris H D'Souza
Blueberry proanthocyanidins (B-PAC) are known to decrease titers of human norovirus surrogates in vitro. The application of B-PAC as therapeutic or preventive options against foodborne viral illness needs to be determined using model foods and simulated gastric conditions in vitro. The objective of this study was to evaluate the antiviral effect of B-PAC in model foods (apple juice (AJ) and 2% reduced fat milk) and simulated gastrointestinal fluids against cultivable human norovirus surrogates (feline calicivirus; FCV-F9 and murine norovirus; MNV-1) over 24 h at 37 °C...
May 2017: Food Microbiology
https://www.readbyqxmd.com/read/28035151/effect-of-concentration-homogenization-and-stabilizing-salts-on-heat-stability-and-rheological-properties-of-cow-skim-milk-ultrafiltered-retentate
#5
Ganga Sahay Meena, Ashish Kumar Singh, Sanket Borad, Narender Raju Panjagari
Ultrafiltration (UF) of skimmed milk altered the composition of UF retentate and decreased the heat stability. Heat stability further reduced upon its subsequent homogenization or diafiltration. Poor heat stability of UF retentate restricts its processing at elevated temperatures. Therefore, this study was aimed to investigate the effect of protein concentration, homogenization and addition of stabilizing salts on the heat stability and rheological properties of UF retentates. Changes in the heat stability of fivefold homogenized UF retentate (5× HUFR) was studied in the pH range of 6...
November 2016: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28001223/in-vitro-antischistosomal-activity-of-venom-from-the-egyptian-snake-cerastes-cerastes
#6
Ehssan Ahmed Hassan, Mohamed Ahmed Abdel-Rahman, Mohamed Moussa Ibrahim, Maha Farid Mohamed Soliman
INTRODUCTION: We studied the potential in vitro antischistosomal activity of Cerastes cerastes venom on adult Schistosoma mansoni worms. METHODS: Live specimens of the horned viper snake, C. cerastes were collected from the Aswan Governorate (Egypt). Venom was collected from snakes by manual milking. Worms of S. mansoni were obtained from infected hamsters by perfusion and isolated from blood using phosphate buffer. Mortality rates of worms were monitored after 3 days of exposure to snake venom at LC50 and various sublethal concentrations (10, 5, 2...
November 2016: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/27989532/comparative-detection-and-recovery-of-pseudomonas-aeruginosa-by-membrane-filtration-and-a-most-probable-number-technique
#7
G A Ngwa, R Schop, J Chow, L Lukic, K McKague
Two reference membrane filtration methods (Standard Methods: SM 9213E and U.S.EPA), the Most Probable Number method (Pseudalert®) and a membrane filtration method currently used by the Ontario Ministry of the Environment and Climate Change (MOECC) Laboratory Services Branch Etobicoke, Canada (Method E3371) were compared for the detection and recovery of Pseudomonas aeruginosa (P. aeruginosa) in sterile phosphate buffered dilution water (PBDW), un-spiked and spiked environmental water samples. The confirmation of presumptive-positive P...
December 15, 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27960182/calcium-phosphate-osteopontin-particles-reduce-biofilm-formation-and-ph-drops-in-in-situ-grown-dental-biofilms
#8
Sebastian Schlafer, Casper J S Ibsen, Henrik Birkedal, Bente Nyvad
This 2-period crossover study investigated the effect of calcium-phosphate-osteopontin particles on biofilm formation and pH in 48-h biofilms grown in situ. Bovine milk osteopontin is a highly phosphorylated glycoprotein that has been shown to interfere with bacterial adhesion to salivary-coated surfaces. Calcium-phosphate-osteopontin particles have been shown to reduce biofilm formation and pH drops in a 5-species laboratory model of dental biofilm without affecting bacterial viability. Here, smooth surface biofilms from 10 individuals were treated ex vivo 6 times/day for 30 min with either calcium-phosphate-osteopontin particles or sterile saline...
December 14, 2016: Caries Research
https://www.readbyqxmd.com/read/27902414/modulation-of-intestinal-barrier-function-to-ameliorate-salmonella-infection-in-mice-by-oral-administration-of-fermented-milks-produced-with-lactobacillus-plantarum-mtcc-5690-a-probiotic-strain-of-indian-gut-origin
#9
Namita Rokana, Rajbir Singh, Rashmi Hogarehalli Mallappa, Virender Kumar Batish, Sunita Grover
Probiotic Lactobacillus plantarum MTCC 5690, a probiotic strain of Indian gut origin, and milk formulations produced with the same were explored in this study as biotherapeutics by evaluating their functional efficacy against Salmonella infection in mice. The efficacy of milk formulations (fermented/unfermented) of MTCC 5690 for enhancement of intestinal barrier function was determined by monitoring the permeability and histopathology of the intestine. Infected mice fed with probiotic Dahi, fermented probiotic drink and sweetened fermented probiotic drink maintained the health and integrity of the intestinal epithelium as compared to those fed with PBS, milk, unfermented probiotic milk and Dahi...
December 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27889170/drying-parameters-greatly-affect-the-destruction-of-cronobacter-sakazakii-and-salmonella-typhimurium-in-standard-buffer-and-milk
#10
Emilie Lang, Cyril Iaconelli, Fiona Zoz, Stéphane Guyot, Pablo Alvarez-Martin, Laurent Beney, Jean-Marie Perrier-Cornet, Patrick Gervais
Salmonella Typhimurium and Cronobacter sakazakii are two foodborne pathogens involved in neonatal infections from milk powder and infant formula. Their ability to survive in low-moisture food and during processing from the decontamination to the dried state is a major issue in food protection. In this work, we studied the effects of the drying process on Salmonella Typhimurium and Cronobacter sakazakii, with the aim of identifying the drying parameters that could promote greater inactivation of these two foodborne pathogens...
April 2017: Food Microbiology
https://www.readbyqxmd.com/read/27889146/the-potential-of-species-specific-tagatose-6-phosphate-t6p-pathway-in-lactobacillus-casei-group-for-galactose-reduction-in-fermented-dairy-foods
#11
Qinglong Wu, Nagendra P Shah
Residual lactose and galactose in fermented dairy foods leads to several industrial and health concerns. There is very little information pertaining to manufacture of fermented dairy foods that are low in lactose and galactose. In the present study, comparative genomic survey demonstrated the constant presence of chromosome-encoded tagatose-6-phosphate (T6P) pathway in Lactobacillus casei group. Lactose/galactose utilization tests and β-galactosidase assay suggest that PTS(Gal) system, PTS(Lac) system and T6P pathway are major contributors for lactose/galactose catabolism in this group of organisms...
April 2017: Food Microbiology
https://www.readbyqxmd.com/read/27872722/production-of-monoclonal-antibody-against-recombinant-ns3-protein-of-bovine-viral-diarrhea-virus-nadl-strain
#12
Masood Reza Seyfi Abad Shapouri, Maryam Ekhtelat, Masood Ghorbanpoor Najaf Abadi, Mohsen Lotfi, Mohamad Rashno
This study was conducted to investigate the prevalence of subclinical mastitis caused by Staphylococcus spp. in ewes in West-Azerbaijan province of Iran. Molecular characterization of isolated Staphylococcus spp. from diseased ewes were performed using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) and DNA sequencing of glyceraldehyde-3-phosphate dehydrogenase (gap) gene. Also, antibiotic resistance of staphylococcal isolates against different antibiotics was investigated...
2016: Veterinary Research Forum
https://www.readbyqxmd.com/read/27849624/efficacy-of-micellized-vs-fat-soluble-vitamin-d3-supplementation-in-healthy-school-children-from-northern-india
#13
Raman K Marwaha, Vamsi K Yenamandra, Mohammed Asraf Ganie, Gomathy Sethuraman, Vishnubhatla Sreenivas, Lakshmy Ramakrishnan, Sathish K Mathur, Vinod K Sharma, Ambrish Mithal
BACKGROUND: Vitamin D deficiency is a widely recognized public health problem. Efficacy of a recently developed micellized form of vitamin D3 has not been studied. Hence, we undertook this study to compare its efficacy with the conventionally used fat-soluble vitamin D3. METHODS: In this open-labeled nonrandomized pilot study, we recruited 180 healthy children, aged 13-14 years in two groups and supplemented Group A (60 children) with 60,000 IU of fat-soluble vitamin D3/month with milk and Group B (120 children) with 60,000 IU/month of water miscible vitamin D3 under supervision for 6 months...
December 1, 2016: Journal of Pediatric Endocrinology & Metabolism: JPEM
https://www.readbyqxmd.com/read/27821208/transformation-kinetics-of-fermented-milk-using-lactobacillus-casei-lc1-and-streptococcus-thermophilus-comparison-of-results-with-other-inocula
#14
Susana Vargas Muñoz, Francisco Quintanilla Guerrero, Maykel González Torres, Ma Del Pilar Carreón Castro, Rogelio Rodríguez Talavera
Probiotic-based starter cultures are generally used to produce fermented milks with improved characteristics in the final product. In this study, Lactobacillus casei and Streptococcus thermophilus (Lc1-St) were used as the starter inoculum. The transformation kinetics and properties of the final product were compared with systems produced with other inocula. The Lc1-St inoculum delayed the production of lactic acid from 40 to 70 min (depending on temperature and concentration) when compared to Lactobacillus delbrueckii ssp...
November 8, 2016: Journal of Dairy Research
https://www.readbyqxmd.com/read/27818678/transcriptome-wide-analysis-reveals-the-role-of-ppar%C3%AE-controlling-the-lipid-metabolism-in-goat-mammary-epithelial-cells
#15
Hengbo Shi, Wangsheng Zhao, Changhui Zhang, Khuram Shahzad, Jun Luo, Juan J Loor
To explore the large-scale effect of peroxisome proliferator-activated receptor γ (PPARG) in goat mammary epithelial cells (GMEC), an oligonucleotide microarray platform was used for transcriptome profiling in cells overexpressing PPARG and incubated with or without rosiglitazone (ROSI, a PPARγ agonist). A total of 1143 differentially expressed genes (DEG) due to treatment were detected. The Dynamic Impact Approach (DIA) analysis uncovered the most impacted and induced pathways "fatty acid elongation in mitochondria," "glycosaminoglycan biosynthesis-keratan sulfate," and "pentose phosphate pathway...
2016: PPAR Research
https://www.readbyqxmd.com/read/27816241/immune-response-after-an-experimental-intramammary-challenge-with-killed-staphylococcus-aureus-in-cows-and-heifers-vaccinated-and-not-vaccinated-with-startvac-a-polyvalent-mastitis-vaccine
#16
S Piepers, A Prenafeta, J Verbeke, A De Visscher, Ricard March, S De Vliegher
An experimental trial was conducted to explore the effect of vaccination with a polyvalent vaccine against mastitis (Startvac) on the early immune response after experimental intramammary challenge with a heterologous killed Staphylococcus aureus strain. The effect of vaccination on milk production, clinical signs, quarter milk somatic cell count, milk polymorphonuclear neutrophilic leukocyte (PMN) concentration and viability, the concentration of antigen-specific antibodies [slime associated antigenic complex (SAAC) and J5] and their IgG1 and IgG2 subtypes in both serum and whey, and the antigen-specific IFN-γ, IL-4, and IL-17 production by blood lymphocytes after in vitro stimulation with S...
January 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/27774728/mycoestrogen-determination-in-cow-milk-magnetic-solid-phase-extraction-followed-by-liquid-chromatography-and-tandem-mass-spectrometry-analysis
#17
Anna Laura Capriotti, Chiara Cavaliere, Patrizia Foglia, Giorgia La Barbera, Roberto Samperi, Salvatore Ventura, Aldo Laganà
Recently, magnetic solid-phase extraction has gained interest because it presents various operational advantages over classical solid-phase extraction. Furthermore, magnetic nanoparticles are easy to prepare, and various materials can be used in their synthesis. In the literature, there are only few studies on the determination of mycoestrogens in milk, although their carryover in milk has occurred. In this work, we wanted to develop the first (to the best of our knowledge) magnetic solid-phase extraction protocol for six mycoestrogens from milk, followed by liquid chromatography and tandem mass spectrometry analysis...
December 2016: Journal of Separation Science
https://www.readbyqxmd.com/read/27723378/microstructured-%C3%AE-tricalcium-phosphate-putty-versus-autologous-bone-for-repair-of-alveolar-clefts-in-a-goat-model
#18
Nard G Janssen, Adrianus P de Ruiter, Wouter M M T van Hout, Vincent van Miegem, Debby Gawlitta, Florence Barrère-de Groot, Gert J Meijer, Antoine J W P Rosenberg, Ronald Koole
For the first time it was demonstrated that an osteoinductive calcium phosphate-based putty is effective in the restoration of complex maxillofacial defects. In these defects, adequate mechanical confinement by multiple bony walls and osteoconduction from multiple surfaces are usually lacking. This study compares the efficacy of a microstructured beta-tricalcium phosphate (β-TCP) putty with autologous bone for the repair of alveolar cleft defects. A total of 10 Dutch milk goats were operated on in a split-mouth study design in which two-wall bony alveolar clefts were created and successively repaired with autologous bone (the gold standard) at one side and β-TCP putty at the other...
October 10, 2016: Cleft Palate-craniofacial Journal
https://www.readbyqxmd.com/read/27692716/milk-acidification-to-control-the-growth-of-mycoplasma-bovis-and-salmonella-dublin-in-contaminated-milk
#19
A M Parker, J K House, M S Hazelton, K L Bosward, V L Mohler, F P Maunsell, P A Sheehy
Bacterial contamination of milk fed to calves compromises calf health. Several bacterial pathogens that infect cows, including Mycoplasma bovis and Salmonella enterica ssp. enterica serovar Dublin, are shed in milk, providing a possible route of transmission to calves. Milk acidification lowers the milk pH so that it is unsuitable for bacterial growth and survival. The objectives of this study were to (1) determine the growth of M. bovis and Salmonella Dublin in milk, and (2) evaluate the efficacy of milk acidification using a commercially available acidification agent (Salstop, Impextraco, Heist-op-den-Berg, Belgium) to control M...
September 28, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27684504/risks-profile-and-health-vulnerability-of-cotton-picker-s-women-by-organanochlorine-phosphates-ocps-from-southern-punjab-pakistan
#20
Humaira Yasmeen, Abdul Qadir, Mehvish Mumtaz, Syed Ali Musstjab Akber Shah Eqani, Jabbir Hussain Syed, Adeel Mahmood, Nadia Jamil, Farva Nazar, Habib Ali, Muhammad Shafiq Ahmad, Zafar Iqbal Tanveer, Gan Zhang
The present study was conducted to highlight the existing level of ∑16 OCPs from human milk (n = 45) and blood serum (n = 40) of cotton picker women in Khanewal district, Southern Punjab, Pakistan. Source apportionment, congener specific analysis and risk surveillance of OCPs was reported from human milk and blood samples. Levels of OCPs (ng g(-1) ) in milk and blood serum samples ranged from 15.7 to 538.3 ng g(-1) and 16.4 to 747. 1 ng g(-1) , respectively and was found lower than previously published reports from other regions of the globe...
September 29, 2016: Environmental Toxicology and Chemistry
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