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https://www.readbyqxmd.com/read/27902414/modulation-of-intestinal-barrier-function-to-ameliorate-salmonella-infection-in-mice-by-oral-administration-of-fermented-milks-produced-with-lactobacillus-plantarum-mtcc-5690-a-probiotic-strain-of-indian-gut-origin
#1
Namita Rokana, Rajbir Singh, Rashmi Hogarehalli Mallappa, Virender Kumar Batish, Sunita Grover
Probiotic Lactobacillus plantarum MTCC 5690 -a probiotic strain of Indian gut origin as such and milk formulations produced with the same were explored in this sudy as biotherapeutics by evauating their functional efficacy against Salmonella infection in mice. The efficacy of milk formulations (fermented/unfermented) of MTCC 5690 for enhancement of intestinal barrier function was determined by monitoring the permeability and histopathology of intestine. The mice fed with probiotic Dahi (PD), Fermented Probiotic Drink (FD) and Sweetened fermented probiotic drink (SFD) maintained the health and integrity of intestinal epithelium in the infected mice as compared to those fed with phosphate buffered saline, milk, unfermented probiotic milk and Dahi...
October 17, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27889170/drying-parameters-greatly-affect-the-destruction-of-cronobacter-sakazakii-and-salmonella-typhimurium-in-standard-buffer-and-milk
#2
Emilie Lang, Cyril Iaconelli, Fiona Zoz, Stéphane Guyot, Pablo Alvarez-Martin, Laurent Beney, Jean-Marie Perrier-Cornet, Patrick Gervais
Salmonella Typhimurium and Cronobacter sakazakii are two foodborne pathogens involved in neonatal infections from milk powder and infant formula. Their ability to survive in low-moisture food and during processing from the decontamination to the dried state is a major issue in food protection. In this work, we studied the effects of the drying process on Salmonella Typhimurium and Cronobacter sakazakii, with the aim of identifying the drying parameters that could promote greater inactivation of these two foodborne pathogens...
April 2017: Food Microbiology
https://www.readbyqxmd.com/read/27889146/the-potential-of-species-specific-tagatose-6-phosphate-t6p-pathway-in-lactobacillus-casei-group-for-galactose-reduction-in-fermented-dairy-foods
#3
Qinglong Wu, Nagendra P Shah
Residual lactose and galactose in fermented dairy foods leads to several industrial and health concerns. There is very little information pertaining to manufacture of fermented dairy foods that are low in lactose and galactose. In the present study, comparative genomic survey demonstrated the constant presence of chromosome-encoded tagatose-6-phosphate (T6P) pathway in Lactobacillus casei group. Lactose/galactose utilization tests and β-galactosidase assay suggest that PTS(Gal) system, PTS(Lac) system and T6P pathway are major contributors for lactose/galactose catabolism in this group of organisms...
April 2017: Food Microbiology
https://www.readbyqxmd.com/read/27872722/production-of-monoclonal-antibody-against-recombinant-ns3-protein-of-bovine-viral-diarrhea-virus-nadl-strain
#4
Masood Reza Seyfi Abad Shapouri, Maryam Ekhtelat, Masood Ghorbanpoor Najaf Abadi, Mohsen Lotfi, Mohamad Rashno
This study was conducted to investigate the prevalence of subclinical mastitis caused by Staphylococcus spp. in ewes in West-Azerbaijan province of Iran. Molecular characterization of isolated Staphylococcus spp. from diseased ewes were performed using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) and DNA sequencing of glyceraldehyde-3-phosphate dehydrogenase (gap) gene. Also, antibiotic resistance of staphylococcal isolates against different antibiotics was investigated...
2016: Veterinary Research Forum
https://www.readbyqxmd.com/read/27849624/efficacy-of-micellized-vs-fat-soluble-vitamin-d3-supplementation-in-healthy-school-children-from-northern-india
#5
Raman K Marwaha, Vamsi K Yenamandra, Mohammed Asraf Ganie, Gomathy Sethuraman, Vishnubhatla Sreenivas, Lakshmy Ramakrishnan, Sathish K Mathur, Vinod K Sharma, Ambrish Mithal
BACKGROUND: Vitamin D deficiency is a widely recognized public health problem. Efficacy of a recently developed micellized form of vitamin D3 has not been studied. Hence, we undertook this study to compare its efficacy with the conventionally used fat-soluble vitamin D3. METHODS: In this open-labeled nonrandomized pilot study, we recruited 180 healthy children, aged 13-14 years in two groups and supplemented Group A (60 children) with 60,000 IU of fat-soluble vitamin D3/month with milk and Group B (120 children) with 60,000 IU/month of water miscible vitamin D3 under supervision for 6 months...
December 1, 2016: Journal of Pediatric Endocrinology & Metabolism: JPEM
https://www.readbyqxmd.com/read/27821208/transformation-kinetics-of-fermented-milk-using-lactobacillus-casei-lc1-and-streptococcus-thermophilus-comparison-of-results-with-other-inocula
#6
Susana Vargas Muñoz, Francisco Quintanilla Guerrero, Maykel González Torres, Ma Del Pilar Carreón Castro, Rogelio Rodríguez Talavera
Probiotic-based starter cultures are generally used to produce fermented milks with improved characteristics in the final product. In this study, Lactobacillus casei and Streptococcus thermophilus (Lc1-St) were used as the starter inoculum. The transformation kinetics and properties of the final product were compared with systems produced with other inocula. The Lc1-St inoculum delayed the production of lactic acid from 40 to 70 min (depending on temperature and concentration) when compared to Lactobacillus delbrueckii ssp...
November 8, 2016: Journal of Dairy Research
https://www.readbyqxmd.com/read/27818678/transcriptome-wide-analysis-reveals-the-role-of-ppar%C3%AE-controlling-the-lipid-metabolism-in-goat-mammary-epithelial-cells
#7
Hengbo Shi, Wangsheng Zhao, Changhui Zhang, Khuram Shahzad, Jun Luo, Juan J Loor
To explore the large-scale effect of peroxisome proliferator-activated receptor γ (PPARG) in goat mammary epithelial cells (GMEC), an oligonucleotide microarray platform was used for transcriptome profiling in cells overexpressing PPARG and incubated with or without rosiglitazone (ROSI, a PPARγ agonist). A total of 1143 differentially expressed genes (DEG) due to treatment were detected. The Dynamic Impact Approach (DIA) analysis uncovered the most impacted and induced pathways "fatty acid elongation in mitochondria," "glycosaminoglycan biosynthesis-keratan sulfate," and "pentose phosphate pathway...
2016: PPAR Research
https://www.readbyqxmd.com/read/27816241/immune-response-after-an-experimental-intramammary-challenge-with-killed-staphylococcus-aureus-in-cows-and-heifers-vaccinated-and-not-vaccinated-with-startvac-a-polyvalent-mastitis-vaccine
#8
S Piepers, A Prenafeta, J Verbeke, A De Visscher, Ricard March, S De Vliegher
An experimental trial was conducted to explore the effect of vaccination with a polyvalent vaccine against mastitis (Startvac) on the early immune response after experimental intramammary challenge with a heterologous killed Staphylococcus aureus strain. The effect of vaccination on milk production, clinical signs, quarter milk somatic cell count, milk polymorphonuclear neutrophilic leukocyte (PMN) concentration and viability, the concentration of antigen-specific antibodies [slime associated antigenic complex (SAAC) and J5] and their IgG1 and IgG2 subtypes in both serum and whey, and the antigen-specific IFN-γ, IL-4, and IL-17 production by blood lymphocytes after in vitro stimulation with S...
November 2, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27774728/mycoestrogen-determination-in-cow-milk-magnetic-solid-phase-extraction-followed-by-liquid-chromatography-and-tandem-mass-spectrometry-analysis
#9
Anna Laura Capriotti, Chiara Cavaliere, Patrizia Foglia, Giorgia La Barbera, Roberto Samperi, Salvatore Ventura, Aldo Laganà
Recently, magnetic solid-phase extraction has gained interest because it presents various operational advantages over classical solid-phase extraction. Furthermore, magnetic nanoparticles are easy to prepare, and various materials can be used in their synthesis. In the literature, there are only few studies on the determination of mycoestrogens in milk, although their carryover in milk has occurred. In this work, we wanted to develop the first (to the best of our knowledge) magnetic solid-phase extraction protocol for six mycoestrogens from milk, followed by liquid chromatography and tandem mass spectrometry analysis...
October 24, 2016: Journal of Separation Science
https://www.readbyqxmd.com/read/27723378/microstructured-%C3%AE-tricalcium-phosphate-putty-versus-autologous-bone-for-repair-of-alveolar-clefts-in-a-goat-model
#10
Nard G Janssen, Adrianus P de Ruiter, Wouter M M T van Hout, Vincent van Miegem, Debby Gawlitta, Florence Barrère-de Groot, Gert J Meijer, Antoine J W P Rosenberg, Ronald Koole
For the first time it was demonstrated that an osteoinductive calcium phosphate-based putty is effective in the restoration of complex maxillofacial defects. In these defects, adequate mechanical confinement by multiple bony walls and osteoconduction from multiple surfaces are usually lacking. This study compares the efficacy of a microstructured beta-tricalcium phosphate (β-TCP) putty with autologous bone for the repair of alveolar cleft defects. A total of 10 Dutch milk goats were operated on in a split-mouth study design in which two-wall bony alveolar clefts were created and successively repaired with autologous bone (the gold standard) at one side and β-TCP putty at the other...
October 10, 2016: Cleft Palate-craniofacial Journal
https://www.readbyqxmd.com/read/27692716/milk-acidification-to-control-the-growth-of-mycoplasma-bovis-and-salmonella-dublin-in-contaminated-milk
#11
A M Parker, J K House, M S Hazelton, K L Bosward, V L Mohler, F P Maunsell, P A Sheehy
Bacterial contamination of milk fed to calves compromises calf health. Several bacterial pathogens that infect cows, including Mycoplasma bovis and Salmonella enterica ssp. enterica serovar Dublin, are shed in milk, providing a possible route of transmission to calves. Milk acidification lowers the milk pH so that it is unsuitable for bacterial growth and survival. The objectives of this study were to (1) determine the growth of M. bovis and Salmonella Dublin in milk, and (2) evaluate the efficacy of milk acidification using a commercially available acidification agent (Salstop, Impextraco, Heist-op-den-Berg, Belgium) to control M...
September 28, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27684504/risks-profile-and-health-vulnerability-of-cotton-picker-s-women-by-organanochlorine-phosphates-ocps-from-southern-punjab-pakistan
#12
Humaira Yasmeen, Abdul Qadir, Mehvish Mumtaz, Syed Ali Musstjab Akber Shah Eqani, Jabbir Hussain Syed, Adeel Mahmood, Nadia Jamil, Farva Nazar, Habib Ali, Muhammad Shafiq Ahmad, Zafar Iqbal Tanveer, Gan Zhang
The present study was conducted to highlight the existing level of ∑16 OCPs from human milk (n = 45) and blood serum (n = 40) of cotton picker women in Khanewal district, Southern Punjab, Pakistan. Source apportionment, congener specific analysis and risk surveillance of OCPs was reported from human milk and blood samples. Levels of OCPs (ng g(-1) ) in milk and blood serum samples ranged from 15.7 to 538.3 ng g(-1) and 16.4 to 747. 1 ng g(-1) , respectively and was found lower than previously published reports from other regions of the globe...
September 29, 2016: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/27628209/sequencing-biological-acidification-of-waste-activated-sludge-aiming-to-optimize-phosphorus-dissolution-and-recovery
#13
Felipe Guilayn, Etienne Braak, Simon Piveteau, Marie-Line Daumer
Phosphorus (P) recovery in wastewater treatment plants (WWTP) as pure crystals such as struvite (MgNH4PO4.6H2O), potassium struvite (KMgPO4.6H2O) and calcium phosphates (e.g. Ca3(PO4)2) is an already feasible technique that permits the production of green and marketable fertilizers and the reduction of operational costs. Commercial crystallizers can recovery more than 90% of soluble P. However, most of the P in WWTP sludge is unavailable for the processes (not dissolved). P solubilization and separation are thus the limiting steps in P-crystallization...
September 14, 2016: Environmental Technology
https://www.readbyqxmd.com/read/27602272/novel-fermented-chickpea-milk-with-enhanced-level-of-%C3%AE-aminobutyric-acid-and-neuroprotective-effect-on-pc12-cells
#14
Wen Li, Mingming Wei, Junjun Wu, Xin Rui, Mingsheng Dong
In this study, novel fermented chickpea milk with high γ -aminobutyric acid (GABA) content and potential neuroprotective activity was developed. Fermentation starter that can produce GABA was selected from 377 strains of lactic acid bacteria isolated from traditional Chinese fermented foods. Among the screened strains, strain M-6 showed the highest GABA-producing capacity in De Man-Rogosa and Sharp (MRS) broth and chickpea milk. M-6 was identified as Lactobacillus plantarum based on Gram staining, API carbohydrate fermentation pattern testing, and 16s rDNA sequencing...
2016: PeerJ
https://www.readbyqxmd.com/read/27581184/synthesis-of-mg-fe-cl-hydrotalcite-like-nanoplatelets-as-an-oral-phosphate-binder-evaluations-of-phosphorus-intercalation-activity-and-cellular-cytotoxicity
#15
Yung-Feng Lung, Ying-Sui Sun, Chun-Kai Lin, Jun-Yen Uan, Her-Hsiung Huang
The patients with end-stage of renal disease (ESRD) need to take oral phosphate binder. Traditional phosphate binders may leave the disadvantage of aluminum intoxication or cardiac calcification. Herein, Mg-Fe-Cl hydrotalcite-like nanoplatelet (HTln) is for the first time characterized as potential oral phosphate binder, with respect to its phosphorus uptake capacity in cow milk and cellular cytotoxicity. A novel method was developed for synthesizing the Mg-Fe-Cl HTln powder in different Mg(2+): Fe(3+) ratios where the optimization was 2...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27562100/acute-and-long-term-outcomes-in-a-drosophila-melanogaster-model-of-classic-galactosemia-occur-independently-of-galactose-1-phosphate-accumulation
#16
Jennifer M I Daenzer, Patricia P Jumbo-Lucioni, Marquise L Hopson, Kerry R Garza, Emily L Ryan, Judith L Fridovich-Keil
Classic galactosemia (CG) is a potentially lethal inborn error of metabolism that results from the profound loss of galactose-1-phosphate uridylyltransferase (GALT), the second enzyme in the Leloir pathway of galactose metabolism. Neonatal detection and dietary restriction of galactose minimizes or resolves the acute sequelae of CG, but fails to prevent the long-term complications experienced by a majority of patients. One of the substrates of GALT, galactose-1-phosphate (Gal-1P), accumulates to high levels in affected infants, especially following milk exposure, and has been proposed as the key mediator of acute and long-term pathophysiology in CG...
November 1, 2016: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/27545286/role-of-phosphate-groups-on-antiviral-activity-of-casein-phosphopeptide-against-feline-calicivirus-as-a-surrogate-for-norovirus
#17
Ntshepisa Lebetwa, Takakazu Mitani, Soichiro Nakamura, Shigeru Katayama
BACKGROUND: Current research on the gastro-intestinal digestion of milk-casein strongly suggests the existence of novel bioactive peptides having antiviral activities that are attributable to their immunostimulatory effects. Here, we investigated the antiviral activity of casein peptides rich in phosphate groups, such as casein phosphopeptide (CPP-III). RESULTS: We prepared two types of CPP with different phosphorylation levels to clarify the role of the phosphate group...
August 22, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27540237/medication-management-of-the-extremely-premature-neonate-the-impact-of-a-specialist-pharmacist
#18
Karen Whitfield, Claudia Barkeij, Angela North
AIM: To present a case of an extremely premature infant and the role that the specialist neonatal pharmacist has on the quality of care of these patients. METHOD: Interventions and recommendations made by the pharmacists over the admission of a triplet born at 23 weeks and 5 days gestation were recorded. The type of interventions were categorised and classified for risk using a consequence/probability matrix.1 RESULTS: The patient required admission to the intensive care unit and subsequently the special care unit for a period of 163 days before discharge home...
September 2016: Archives of Disease in Childhood
https://www.readbyqxmd.com/read/27506711/electrochemical-sensing-of-casein-based-on-the-interaction-between-its-phosphate-groups-and-a-ruthenium-iii-complex
#19
Iku Inaba, Hideki Kuramitz, Kazuharu Sugawara
A reaction to casein, along with β-lactoglobulin, is a main cause of milk allergies, and also is a useful indicator of protein in allergic analyses. In the present study, a simple casein sensor was developed based on the interaction between a phosphate group of casein and electroactive [Ru(NH3)6](3+). We evaluated the voltammetric behavior of a casein-[Ru(NH3)6](3+) complex using a glassy carbon electrode. When the ruthenium(III) complex was combined with the phosphate groups of casein, the structure of the casein was changed...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27491477/revisiting-the-interpretation-of-casein-micelle-saxs-data
#20
B Ingham, A Smialowska, G D Erlangga, L Matia-Merino, N M Kirby, C Wang, R G Haverkamp, A J Carr
An in-depth, critical review of model-dependent fitting of small-angle X-ray scattering (SAXS) data of bovine skim milk has led us to develop a new mathematical model for interpreting these data. Calcium-edge resonant soft X-ray scattering data provides unequivocal evidence as to the shape and location of the scattering due to colloidal calcium phosphate, which is manifested as a correlation peak centred at q = 0.035 Å(-1). In SAXS data this feature is seldom seen, although most literature studies attribute another feature centred at q = 0...
August 17, 2016: Soft Matter
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