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https://www.readbyqxmd.com/read/28945027/-dna-marker-assisted-selection-of-medicinal-plants-%C3%A2-breeding-research-of-disease-resistant-cultivars-of-panax-notoginseng
#1
Min Liu, Wen-Li Huang, Xiao Wang, Rui-Han Wan, Hai Wang, Zhu-Yun Yan
The study is aimed to estimate the diversity of arbuscular mycorrhizal fungi (AMF) in the main producing areas of Salvia miltiorrhiza.Diversity of AMF was surveyed directly on spores isolated from the field soil, collected from 20 sites of 8 provinces. Identification of the AMF was made by observation of spore morphology. At least 27 recognized AMF species were identified in samples from field soil, belonging to seven genera of AMF-Acaulospora, Glomus, Funneliformis, Ambispora, Rhizophagus, Pacispora, and Claroideoglomus...
January 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28945015/membrane-fusion-mediated-intracellular-delivery-of-lipid-bilayer-coated-mesoporous-silica-nanoparticles
#2
Jian Yang, Jing Tu, Gerda E M Lamers, René C L Olsthoorn, Alexander Kros
Protein delivery into the cytosol of cells is a challenging topic in the field of nanomedicine, because cellular uptake and endosomal escape are typically inefficient, hampering clinical applications. In this contribution cuboidal mesoporous silica nanoparticles (MSNs) containing disk-shaped cavities with a large pore diameter (10 nm) are studied as a protein delivery vehicle using cytochrome-c (cytC) as a model membrane-impermeable protein. To ensure colloidal stability, the MSNs are coated with a fusogenic lipid bilayer (LB) and cellular uptake is induced by a complementary pair of coiled-coil (CC) lipopeptides...
September 25, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28944857/a-novel-fbn1-mutation-causes-autosomal-dominant-marfan-syndrome
#3
Ying Xiao, Xiaoqi Liu, Xiaoxin Guo, Liping Liu, Linxin Jiang, Qi Wang, Bo Gong
Marfan syndrome (MFS) is an inherited and systemic disorder. It has been reported that mutations in the fibrillin‑1 gene (FBN1) account for ~90% of autosomal dominant cases of MFS. This study was conducted to screen mutations of FBN1 in a Chinese family with autosomal dominant MFS; four individuals including two patients with MFS were recruited. The family members underwent complete physical, cardiovascular and ophthalmologic examinations. Genomic DNA samples were collected from the family along with 383 unrelated healthy subjects...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944810/microfluidic-step-emulsification-in-axisymmetric-geometry
#4
I Chakraborty, J Ricouvier, P Yazhgur, P Tabeling, A M Leshansky
Biphasic step-emulsification (Z. Li et al., Lab Chip, 2015, 15, 1023) is a promising microfluidic technique for high-throughput production of μm and sub-μm highly monodisperse droplets. The step-emulsifier consists of a shallow (Hele-Shaw) microchannel operating with two co-flowing immiscible liquids and an abrupt expansion (i.e., step) to a deep and wide reservoir. Under certain conditions the confined stream of the disperse phase, engulfed by the co-flowing continuous phase, breaks into small highly monodisperse droplets at the step...
September 25, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28944802/subcellular-compartmentalisation-of-copper-iron-manganese-and-zinc-in-the-parkinson-s-disease-brain
#5
Sian Genoud, Blaine R Roberts, Adam P Gunn, Glenda M Halliday, Simon J G Lewis, Helen J Ball, Dominic J Hare, Kay L Double
Elevated iron and decreased copper levels are cardinal features of the degenerating substantia nigra pars compacta in the Parkinson's disease brain. Both of these redox-active metals, and fellow transition metals manganese and zinc, are found at high concentrations within the midbrain and participate in a range of unique biological reactions. We examined the total metal content and cellular compartmentalisation of manganese, iron, copper and zinc in the degenerating substantia nigra, disease-affected but non-degenerating fusiform gyrus, and unaffected occipital cortex in the post mortem Parkinson's disease brain compared with age-matched controls...
September 25, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28944752/a-randomised-controlled-study-of-the-effectiveness-of-breathing-retraining-exercises-taught-by-a-physiotherapist-either-by-instructional-dvd-or-in-face-to-face-sessions-in-the-management-of-asthma-in-adults
#6
Mike Thomas, Anne Bruton, Paul Little, Stephen Holgate, Amanda Lee, Lucy Yardley, Steve George, James Raftery, Jennifer Versnel, David Price, Ian Pavord, Ratko Djukanovic, Michael Moore, Sarah Kirby, Guiqing Yao, Shihua Zhu, Emily Arden-Close, Manimekalai Thiruvothiyur, Frances Webley, Mark Stafford-Watson, Elizabeth Dixon, Lynda Taylor
BACKGROUND: Asthma control is suboptimal, resulting in quality of life (QoL) impairment and costs. Breathing retraining exercises have evidence of effectiveness as adjuvant treatment, but are infrequently used. OBJECTIVES: To transfer the contents of a brief (three-session) physiotherapist-delivered breathing retraining programme to a digital versatile disc (DVD) and booklet format; to compare the effectiveness of the self-guided intervention with that of 'face-to-face' physiotherapy and usual care for QoL and other asthma-related outcomes; to perform a health economic assessment of both interventions; and to perform a process evaluation using quantitative and qualitative methods...
September 2017: Health Technology Assessment: HTA
https://www.readbyqxmd.com/read/28944657/wetting-regimes-for-residual-layer-free-transfer-molding-at-the-micro-and-nanoscale
#7
Michael Deagen, Linda S Schadler, Chaitanya K Ullal
Transfer molding offers a low-cost approach to large-area fabrication of isolated structures in a variety of materials when recessed features of the open-faced mold are filled without leaving a residual layer on the plateaus of the mold. Considering both macroscale dewetting and microscale capillary flow, a proposed map of wetting regimes for blade meniscus coating provides a guide for achieving discontinuous dewetting at maximum throughput. Dependence of meniscus morphology on the azimuthal orientation of the stamp provides insight into the dominant mechanisms for discontinuous dewetting of 1-D patterns...
September 25, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28944648/association-of-col11a1-4603c-t-polymorphism-with-cervical-disc-degeneration-in-collegiate-wrestlers
#8
Koji Koyama, Koichi Nakazato, Shogo Maeda, Naoki Kikuchi, Shingo Matsumoto, Kenji Hiranuma
BACKGROUND: The authors previously identified that COL11A1 gene polymorphism is not a susceptibility factor for lumbar disc degeneration (LDD) in athletes. However, the relationship between COL11A1 gene polymorphism and cervical disc degeneration (CDD) remains unclear. We hypothesized that significant associations between COL11A1 4603C/T gene polymorphism and CDD, but not LDD, in collegiate wrestlers exist. This study aims to examine the relationship between CDD, LDD, and COL11A1 4603C/T gene polymorphism in collegiate wrestlers...
September 22, 2017: Journal of Sports Medicine and Physical Fitness
https://www.readbyqxmd.com/read/28944645/effect-of-bcaa-supplement-timing-on-exercise-induced-muscle-soreness-and-damage-a-pilot-placebo-controlled-double-blind-study
#9
Song-Gyu Ra, Teruo Miyazaki, Ryo Kojima, Shoichi Komine, Keisuke Ishikura, Kentaro Kawanaka, Akira Honda, Yasushi Matsuzaki, Hajime Ohmori
BACKGROUND: The aim of present study was to compare the effects of branched-chain amino acid (BCAA) supplementation taken before or after exercise on delayed onset muscle soreness (DOMS) and exercise-induced muscle damage (EIMD). METHODS: Fifteen young men (aged 21.5 ± 0.4 years) were given either BCAA (9.6 g·day-1) or placebo before and after exercise (and for 3 days prior to and following the exercise day) in three independent groups: the Control group (placebo before and after exercise), the PRE group (BCAA before exercise and placebo after exercise), and the POST group (placebo before exercise and BCAA after exercise)...
September 22, 2017: Journal of Sports Medicine and Physical Fitness
https://www.readbyqxmd.com/read/28944635/-unknown-title
#10
(no author information available yet)
The use of a Nursing Model (NM) for nursing administration offers direct and indirect benefits for patients as for nurses. Depending the chosen NM, the concepts of person, health, nursing and environment are very different. Each NM has its special vision of the practice of nursing. The study investigated whether the Chief Nursing Officers (CNO) of the bilingual and French speaking Belgian hospitals integrate Nursing Models in the politics of their department. A quantitative descriptive and correlational survey was conducted...
August 17, 2017: Recherche en Soins Infirmiers
https://www.readbyqxmd.com/read/28944475/the-effect-of-consumer-pressure-and-abiotic-stress-on-positive-plant-interactions-are-mediated-by-extreme-climatic-events
#11
Alessandro Filazzola, Amanda Rae Liczner, Michael Westphal, Christopher J Lortie
Environmental extremes resulting from a changing climate can have profound implications for plant interactions in desert communities. Positive interactions can buffer plant communities from abiotic stress and consumer pressure caused by climatic extremes, but limited research has explored this empirically. We tested the hypothesis that the mechanism of shrub facilitation on an annual plant community can change with precipitation extremes in deserts. During years of extreme drought and above-average rainfall in a desert, we measured plant interactions and biomass while manipulating a soil moisture gradient and reducing consumer pressure...
September 25, 2017: New Phytologist
https://www.readbyqxmd.com/read/28944466/genetic-analysis-reveals-the-diversity-of-larval-gobiidae-in-a-temperate-estuary
#12
H Ojaveer, R Gross, K Laur, T Arula, R Klais
Using molecular tools to examine Gobiidae, the second most abundant taxon in ichthyoplankton samples in the Gulf of Riga (Baltic Sea), the sand goby Pomatoschistus minutus was the most abundant taxon (82% of all individuals analysed), the common goby Pomatoschistus microps constituted 12% and the black goby Gobius niger 6%. The spatiotemporal distribution of P. microps and G. niger indicated a preference for habitats closer to the river inlet and their abundances increased slightly towards the end of the sampling period in summer...
September 25, 2017: Journal of Fish Biology
https://www.readbyqxmd.com/read/28944402/the-impact-of-electron-donors-and-anode-potentials-on-the-anode-respiring-bacteria-community
#13
Xianbin Ying, Kun Guo, Wei Chen, Yuan Gu, Dongsheng Shen, Yuyang Zhou, Yuxiang Liang, Yanfeng Wang, Meizhen Wang, Huajun Feng
Both anode potentials and substrates can affect the process of biofilm formation in bioelectrochemical systems, but it is unclear who primarily determine the anode-respiring bacteria (ARB) community structure and composition. To address this issue, we divided microbial electrolysis cells (MECs) into groups, feeding them with different substrates and culturing them at various potentials. Non-turnover cyclic voltammetry indicated that the extracellular electron transfer components were uniform when feeding acetate, because the same oxidation peaks occurred at - 0...
September 25, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28944392/bonding-and-spectroscopic-analyses-of-n2o-cs2-and-n2o-ocs-heterodimer-complexes-and-their-atmospheric-consequences
#14
Samiyara Begum, Ranga Subramanian
Different isomers of the valence isoelectronic pairs of the heterodimers N2O-SCS and N2O-OCS were investigated using MP2 and CCSD(T) methods with the aug-cc-pVXZ (X = D, T) basis set with anharmonic frequency calculations. Interaction energies of both the heterodimers were estimated at the CBS limit and for CCSD(T)/aug-cc-pVTZ. One isomer was located for the N2O-SCS heterodimer. In the case of the N2O-OCS heterodimer, three planar slipped-parallel isomers were characterized. There was no significant change in electron density at the bond critical points (BCPs) of various intra-bonds but the presence of inter-bonds with a weak electron density at the BCPs in the complexes was found as per the results from the quantum theory of atoms in molecules (QTAIM) analysis...
September 25, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28944299/geographic-genetic-analysis-of-plasmodium-falciparum-parasite-populations-from-surveys-of-primary-school-children-in-western-kenya
#15
Irene Omedo, Polycarp Mogeni, Kirk Rockett, Alice Kamau, Christina Hubbart, Anna Jeffreys, Lynette Isabella Ochola-Oyier, Etienne P de Villiers, Caroline W Gitonga, Abdisalan M Noor, Robert W Snow, Dominic Kwiatkowski, Philip Bejon
Background. Malaria control, and finally malaria elimination, requires the identification and targeting of residual foci or hotspots of transmission. However, the level of parasite mixing within and between geographical locations is likely to impact the effectiveness and durability of control interventions and thus should be taken into consideration when developing control programs. Methods. In order to determine the geographic-genetic patterns of Plasmodium falciparum parasite populations at a sub-national level in Kenya, we used the Sequenom platform to genotype 111 genome-wide distributed single nucleotide polymorphic (SNP) positions in 2486 isolates collected from children in 95 primary schools in western Kenya...
2017: Wellcome Open Research
https://www.readbyqxmd.com/read/28944255/soft-x-ray-absorption-spectroscopy-of-metalloproteins-and-high-valent-metal-complexes-at-room-temperature-using-free-electron-lasers
#16
Markus Kubin, Jan Kern, Sheraz Gul, Thomas Kroll, Ruchira Chatterjee, Heike Löchel, Franklin D Fuller, Raymond G Sierra, Wilson Quevedo, Christian Weniger, Jens Rehanek, Anatoly Firsov, Hartawan Laksmono, Clemens Weninger, Roberto Alonso-Mori, Dennis L Nordlund, Benedikt Lassalle-Kaiser, James M Glownia, Jacek Krzywinski, Stefan Moeller, Joshua J Turner, Michael P Minitti, Georgi L Dakovski, Sergey Koroidov, Anurag Kawde, Jacob S Kanady, Emily Y Tsui, Sandy Suseno, Zhiji Han, Ethan Hill, Taketo Taguchi, Andrew S Borovik, Theodor Agapie, Johannes Messinger, Alexei Erko, Alexander Föhlisch, Uwe Bergmann, Rolf Mitzner, Vittal K Yachandra, Junko Yano, Philippe Wernet
X-ray absorption spectroscopy at the L-edge of 3d transition metals provides unique information on the local metal charge and spin states by directly probing 3d-derived molecular orbitals through 2p-3d transitions. However, this soft x-ray technique has been rarely used at synchrotron facilities for mechanistic studies of metalloenzymes due to the difficulties of x-ray-induced sample damage and strong background signals from light elements that can dominate the low metal signal. Here, we combine femtosecond soft x-ray pulses from a free-electron laser with a novel x-ray fluorescence-yield spectrometer to overcome these difficulties...
September 2017: Structural Dynamics (Melville, N.Y.)
https://www.readbyqxmd.com/read/28944242/autosomal-recessive-long-qt-syndrome-type-1-in-eight-families-from-saudi-arabia
#17
Amnah Y Bdier, Saleh Al-Ghamdi, Prashant K Verma, Khalid Dagriri, Bandar Alshehri, Omamah A Jiman, Sherif E Ahmed, Arthur A M Wilde, Zahurul A Bhuiyan, Jumana Y Al-Aama
BACKGROUND: One of the most common primary cardiac arrhythmia syndromes is autosomal dominant long QT syndrome, type 1 (LQT1), chiefly caused by mono-allelic mutations in the KCNQ1 gene. Bi-allelic mutations in the KCNQ1 gene are causal to Jervell and Lange-Nielsen syndrome (JLNS), characterized by severe and early-onset arrhythmias with prolonged QTc interval on surface ECG and sensorineural deafness. Occasionally, bi-allelic mutations in KCNQ1 are also found in patients without any deafness, referred to as autosomal recessive long QT syndrome, type 1 (AR LQT1)...
September 2017: Molecular Genetics & Genomic Medicine
https://www.readbyqxmd.com/read/28944235/assessing-risk-for-mendelian-disorders-in-a-bronx-population
#18
Guy diSibio, Kinnari Upadhyay, Philip Meyer, Carole Oddoux, Harry Ostrer
BACKGROUND: To identify variants likely responsible for Mendelian disorders among the three major ethnic groups in the Bronx that might be useful to include in genetic screening panels or whole exome sequencing filters and to estimate their likely prevalence in these populations. METHODS: Variants from a high-density oligonucleotide screen of 192 members from each of the three ethnic-national populations (African Americans, Puerto Ricans, and Dominicans) were evaluated for overlap with next generation sequencing data...
September 2017: Molecular Genetics & Genomic Medicine
https://www.readbyqxmd.com/read/28944133/photophysiological-and-light-absorption-properties-of-phytoplankton-communities-in-the-river-dominated-margin-of-the-northern-gulf-of-mexico
#19
Sumit Chakraborty, Steven E Lohrenz, Kjell Gundersen
Spatial and temporal variability in photophysiological properties of phytoplankton were examined in relationship to phytoplankton community composition in the river-dominated continental margin of the northern Gulf of Mexico (NGOM). Observations made during five research cruises in the NGOM included phytoplankton photosynthetic and optical properties and associated environmental conditions and phytoplankton community structure. Distinct patterns of spatial and temporal variability in photophysiological parameters were found for waters dominated by different phytoplankton groups...
June 2017: Journal of Geophysical Research. Oceans
https://www.readbyqxmd.com/read/28944034/rhododendron-aureum-georgi-formed-a-special-soil-microbial-community-and-competed-with-above-ground-plants-on-the-tundra-of-the-changbai-mountain-china
#20
Xiaolong Wang, Lin Li, Wei Zhao, Jiaxin Zhao, Xia Chen
Rhododendron aureum Georgi is a perennial evergreen dwarf shrub that grows at all elevations within the alpine tundra of northern China. Previous research has investigated the plant communities of R. aureum; however, little information is available regarding interspecific competition and underground soil microbial community composition. The objective of our study was to determine whether the presence of R. aureum creates a unique soil microbiome and to investigate the relationship between R. aureum and other plant species...
September 2017: Ecology and Evolution
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