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https://www.readbyqxmd.com/read/29765237/comparative-genomic-analysis-of-multidrug-resistant-streptococcus-pneumoniae-isolates
#1
Fen Pan, Hong Zhang, Xiaoyan Dong, Weixing Ye, Ping He, Shulin Zhang, Jeff Xianchao Zhu, Nanbert Zhong
Introduction: Multidrug resistance in Streptococcus pneumoniae has emerged as a serious problem to public health. A further understanding of the genetic diversity in antibiotic-resistant S. pneumoniae isolates is needed. Methods: We conducted whole-genome resequencing for 25 pneumococcal strains isolated from children with different antimicrobial resistance profiles. Comparative analysis focus on detection of single-nucleotide polymorphisms (SNPs) and insertions and deletions (indels) was conducted...
2018: Infection and Drug Resistance
https://www.readbyqxmd.com/read/29652837/fabrication-of-composite-microneedle-array-electrode-for-temperature-and-bio-signal-monitoring
#2
Yiwei Sun, Lei Ren, Lelun Jiang, Yong Tang, Bin Liu
Body temperature and bio-signals are important health indicators that reflect the human health condition. However, monitoring these indexes is inconvenient and time-consuming, requires various instruments, and needs professional skill. In this study, a composite microneedle array electrode (CMAE) was designed and fabricated. It simultaneously detects body temperature and bio-signals. The CMAE consists of a 6 × 6 microneedles array with a height of 500 μm and a base diameter of 200 μm. Multiple insertion experiments indicate that the CMAE possesses excellent mechanical properties...
April 13, 2018: Sensors
https://www.readbyqxmd.com/read/29465251/managing-iron-deficiency-and-iron-deficiency-anemia-in-inflammatory-bowel-disease-the-results-of-the-gestiona-hierro-eii-survey
#3
Francesc Casellas Jordá, Isabel Vera Mendoza, Manuel Barreiro-de Acosta, Juan María Vázquez Morón, Javier López Román, Javier Júdez Gutiérrez
INTRODUCTION: iron deficiency anemia is a common and very relevant manifestation of inflammatory bowel disease (IBD). Although clinical practice guidelines have been published and updated on this subject, the management in the daily practice of this complication is far from optimal. OBJECTIVE: to determine the actual management, needs and limitations of anemia in IBD by means of a survey of gastroenterology specialists. MATERIAL AND METHODS: a self-administered telematic survey was carried out between April and May 2017 and was sent to SEPD members...
March 2018: Revista Española de Enfermedades Digestivas
https://www.readbyqxmd.com/read/29462135/accomplishing-the-genotype-specific-serodiagnosis-of-single-and-dual-trypanosoma-cruzi-infections-by-flow-cytometry-chagas-flow-ate-igg2a
#4
Glaucia Diniz Alessio, Fernanda Fortes de Araújo, Policarpo Ademar Sales Júnior, Matheus de Souza Gomes, Laurence Rodrigues do Amaral, Marcelo Antônio Pascoal Xavier, Andréa Teixeira-Carvalho, Marta de Lana, Olindo Assis Martins-Filho
The methods currently available for genotype-specific diagnosis of T. cruzi infection still present relevant limitations, especially to identify mixed infection. In the present investigation, we have evaluated the performance of Chagas-Flow ATE-IgG2a test for early and late differential diagnosis of single and dual genotype-specific T. cruzi infections. Serum samples from Swiss mice at early and late stages of T. cruzi infection were assayed in parallel batches for genotype-specific diagnosis of single (TcI, TcVI or TcII) and dual (TcI+TcVI, TcVI+TcII or TcII+TcI) infections...
February 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29322591/inter-rater-agreement-of-novel-high-resolution-impedance-manometry-metrics-bolus-flow-time-and-esophageal-impedance-integral-ratio
#5
D A Carlson, Z Lin, W Kou, J E Pandolfino
BACKGROUND: Novel high-resolution impedance manometry (HRIM) metrics of bolus flow time (BFT) and esophageal impedance integral (EII) ratio have demonstrated clinical utility, though the reliability of their analysis has not been assessed. We aimed to evaluate the inter-rater agreement of the BFT and EII ratio. METHODS: HRIM studies including five upright, liquid swallows from 40 adult patients were analyzed by two raters using a customized MATLAB program to generate the BFT and EII ratio...
January 11, 2018: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/29163597/morphology-carbohydrate-distribution-gene-expression-and-enzymatic-activities-related-to-cell-wall-hydrolysis-in-four-barley-varieties-during-simulated-malting
#6
Natalie S Betts, Laura G Wilkinson, Shi F Khor, Neil J Shirley, Finn Lok, Birgitte Skadhauge, Rachel A Burton, Geoffrey B Fincher, Helen M Collins
Many biological processes, such as cell wall hydrolysis and the mobilisation of nutrient reserves from the starchy endosperm, require stringent regulation to successfully malt barley ( Hordeum vulgare ) grain in an industrial context. Much of the accumulated knowledge defining these events has been collected from individual, unrelated experiments, and data have often been extrapolated from Petri dish germination, rather than malting, experiments. Here, we present comprehensive morphological, biochemical, and transcript data from a simulated malt batch of the three elite malting cultivars Admiral, Navigator, and Flagship, and the feed cultivar Keel...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29155168/improved-assessment-of-bolus-clearance-in-patients-with-achalasia-using-high-resolution-impedance-manometry
#7
Dustin A Carlson, Claire A Beveridge, Zhiyue Lin, Michelle Balla, Dyanna Gregory, Michael Tye, Katherine Ritter, Peter J Kahrilas, John E Pandolfino
BACKGROUND & AIMS: Esophageal retention is typically evaluated by timed-barium esophagram in patients treated for achalasia. Esophageal bolus clearance can also be evaluated using high-resolution impedance manometry. We evaluated the associations of conventional and novel high-resolution impedance manometry metrics, esophagram, and patient-reported outcomes (PROs) in achalasia. METHODS: We performed a prospective study of 70 patients with achalasia (age, 20-81 y; 30 women) treated by pneumatic dilation or myotomy who underwent follow-up evaluations from April 2013 through December 2015 (median, 12 mo after treatment; range, 3-183 mo)...
May 2018: Clinical Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28972732/bilayer-plots-for-accurately-determining-the-chirality-of-single-walled-carbon-nanotubes-under-complex-environments
#8
Juan Yang, Daqi Zhang, Yuecong Hu, Chenmaya Xia, Sida Sun, Yan Li
The chirality (n,m) determines all structures and properties of a single-walled carbon nanotube (SWNT), therefore, accurate and convenient (n,m) assignments are vital in nanotube-related science and technology. Previously, a so-called Kataura plot that protracts the excitonic transition energies (Eii's) of SWNTs with various (n,m) with respect to the tube diameter (dt) has been widely utilized by researchers in the nanotube community for all (n,m)-related studies. However, the facts that both Eii and the calculated dt are subject to interactions with the environments make it inconvenient to accurately determine the (n,m) under complex environments...
October 24, 2017: ACS Nano
https://www.readbyqxmd.com/read/28821551/coordinated-regulation-of-the-eii-man-and-frurki-operons-of-streptococcus-mutans-by-global-and-fructose-specific-pathways
#9
Lin Zeng, Brinta Chakraborty, Tanaz Farivar, Robert A Burne
The glucose/mannose-phosphotransferase system (PTS) permease EIIMan encoded by manLMN in the dental caries pathogen Streptococcus mutans has a dominant influence on sugar-specific, CcpA-independent catabolite repression (CR). Mutations in manL affect energy metabolism and virulence-associated traits, including biofilm formation, acid tolerance, and competence. Using promoter::reporter fusions, expression of the manLMN and the fruRKI operons, encoding a transcriptional regulator, a fructose-1-phosphate kinase and a fructose-PTS permease EIIFru , respectively, was monitored in response to carbohydrate source and in mutants lacking CcpA, FruR, and components of EIIMan Expression of genes for EIIMan and EIIFru was directly regulated by CcpA and CR, as evinced by in vivo and in vitro methods...
November 1, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28578623/definition-of-type-ii-endoleak-risk-based-on-preoperative-anatomical-characteristics
#10
Michele Piazza, Francesco Squizzato, Tommaso Miccoli, Sandro Lepidi, Mirko Menegolo, Franco Grego, Michele Antonello
PURPOSE: To define the risk for type II endoleak (EII) after endovascular aneurysm repair (EVAR) based on preoperative anatomical characteristics. METHODS: Between January 2008 and December 2015, 189 patients (mean age 78.4±7.6 years; 165 men) underwent standard EVAR. Mean aneurysm diameter was 5.7±0.7 cm and mean volume 125.2±45.8 cm(3). Patients were assigned to the "at-risk" group (n=123, 65%) when at least one of the following criteria was present: patency of a >3-mm inferior mesenteric artery (IMA), patency of at least 3 pairs of lumbar arteries, or patency of 2 pairs of lumbar arteries and a sacral artery or accessory renal artery or any diameter patent IMA; otherwise, patients were entered in the "low-risk" group (n=66, 35%)...
August 2017: Journal of Endovascular Therapy
https://www.readbyqxmd.com/read/28578504/focus-on-o-phosphohydroxylysine-o-phosphohydroxyproline-n-1-phosphotryptophan-and-s-phosphocysteine
#11
REVIEW
Matthew J Piggott, Paul V Attwood
The synthesis and chemistry of the lesser-known phosphoamino acids, O-phosphohydroxylysine, O-phosphohydroxyproline, N 1 -phosphotryptophan and S-phosphocysteine are described in detail. In addition, where anything at all is known, the biological synthesis, occurrence and functions of these phosphoamino acids are described. Of these phosphoamino acids, only N 1 -phosphotryptophan has not been reported to occur in proteins; however, apart from the roles of S-phosphocysteine in the sugar transporter component (EII) and in catalysis by protein phosphotyrosine phosphatase, little is currently known about the biological roles of the phosphoamino acids when they occur as post-translational modifications...
August 2017: Amino Acids
https://www.readbyqxmd.com/read/28479464/embo-evar-a-technique-to-prevent-type-ii-endoleak-a-single-center-experience
#12
Massimiliano Natrella, Alessandro Rapellino, Federico Navarretta, Gianluca Iob, Massimo Cristoferi, Matteo Castagnola, Gianmarco Lunardi, Luca Duc, Gianluca Fanelli, Andrea Peruzzo Cornetto, Teodoro Meloni, Flavio Peinetti
BACKGROUND: Intraprocedural aneurysm sac embolization (embo-EVAR) during endovascular abdominal aneurysm repair (EVAR) using coils and fibrin glue is a technique for preventing type II endoleak (EII). Our aim is to evaluate feasibility, safety and clinical outcome of this promising approach. MATERIALS AND METHODS: A retrospective clinical case analysis of 72 patients who underwent EVAR during the period 2011-2014. Two groups were compared at 6 and 12 months follow-up with contrast media computed tomography scan and contrast-enhanced ultrasound (CEUS) imaging: consecutively, 36 patients (group A) treated with classic EVAR and 36 patients (group B) treated with embo-EVAR...
October 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/28373357/the-legionella-pneumophila-incomplete-phosphotransferase-system-is-required-for-optimal-intracellular-growth-and-maximal-expression-of-pmra-regulated-effectors
#13
Yariv Speiser, Tal Zusman, Anna Pasechnek, Gil Segal
The nitrogen phosphotransferase system (PTSNtr ) is a regulatory cascade present in many bacteria, where it controls different functions. This system is usually composed of three basic components: enzyme INtr (EINtr ), NPr, and EIIANtr (encoded by the ptsP , ptsO , and ptsN genes, respectively). In Legionella pneumophila , as well as in many other Legionella species, the EIIANtr component is missing. However, we found that deletion mutations in both ptsP and ptsO are partially attenuated for intracellular growth...
June 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28258745/assessment-of-euroscore-and-saps-iii-as-hospital-mortality-predicted-in-cardiac-surgery
#14
B Mateos-Pañero, M Sánchez-Casado, B Castaño-Moreira, I Paredes-Astillero, L F López-Almodóvar, F Bustos-Molina
OBJECTIVES: To perform an external validation of Euroscore I, Euroscore II and SAPS III. PATIENTS AND METHOD: Retrospective cohort study over three years on all adult patients who underwent cardiac surgery. We reviewed the clinical data, following the patient until outcome or discharge from hospital (dead, alive). We computed the predicted mortality by Euroscore I (EI), II (EII) and SAPS III. The model validation was assessed by discrimination: area under curve ROC; and calibration (Hosmer-Lemeshow test)...
May 2017: Revista Española de Anestesiología y Reanimación
https://www.readbyqxmd.com/read/28239779/variation-in-barley-1%C3%A2-%C3%A2-3-1%C3%A2-%C3%A2-%C3%A2-4-%C3%AE-glucan-endohydrolases-reveals-novel-allozymes-with-increased-thermostability
#15
Juanita C Lauer, Suong Cu, Rachel A Burton, Jason K Eglinton
Novel barley (1 → 3, 1 → 4)-β-glucan endohydrolases with increased thermostability. Rapid and reliable degradation of (1 → 3, 1 → 4)-β-glucan to produce low viscosity wort is an essential requirement for malting barley. The (1 → 3, 1 → 4)-β-glucan endohyrolases are responsible for the primary hydrolysis of cell wall β-glucan. The variation in β-glucanase genes HvGlb1 and HvGlb2 that encode EI and EII, respectively, were examined in elite and exotic germplasm. Six EI and 14 EII allozymes were identified, and significant variation was found in β-glucanase from Hordeum vulgare ssp...
May 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/28168775/structural-characterization-of-the-pts-iia-and-iib-proteins-associated-with-pneumococcal-fucose-utilization
#16
Melanie A Higgins, Aileen M Hamilton, Alisdair B Boraston
Streptococcus pneumoniae harbors a significant number of transporters, including phosphotransferase (PTS) systems, allowing the bacterium to utilize a number of different carbohydrates for metabolic and other purposes. The genes encoding for one PTS transport system in particular (EII(fuc) ) are found within a fucose utilization operon in S. pneumoniae TIGR4. Here, we report the three-dimensional structures of IIA(fuc) and IIB(fuc) providing evidence that this PTS system belongs to the EII(man) family. Additionally, the predicted metabolic pathway for this distinctive fucose utilization system suggests that EII(fuc) transports the H-disaccharide blood group antigen, which would represent a novel PTS transporter specificity...
May 2017: Proteins
https://www.readbyqxmd.com/read/28059636/effects-of-adequate-iodine-supply-on-the-incidence-of-iodine-induced-thyroid-disorders-in-slovenia
#17
Vid Bajuk, Katja Zaletel, Edvard Pirnat, Sergej Hojker, Simona Gaberšček
BACKGROUND: This study aimed to establish the changes in the incidence and characteristics of iodine-induced hyperthyroidism (II-Hyper) and iodine-induced hypothyroidism (II-Hypo) in the two-year period before and the 10-year period after the increase in mandatory salt iodization from the previous 10 mg/kg of potassium iodide to 25 mg/kg in 1999. Furthermore, the aim was to determine the duration of treatment in II-Hyper patients, since no data regarding severity and treatment of II-Hyper with respect to iodine supply are available...
April 2017: Thyroid: Official Journal of the American Thyroid Association
https://www.readbyqxmd.com/read/27936680/novel-barley-1%C3%A2-3-1%C3%A2-4-%C3%AE-glucan-endohydrolase-alleles-confer-increased-enzyme-thermostability
#18
Juanita C Lauer, Kuok Yap, Suong Cu, Rachel A Burton, Jason K Eglinton
Barley (1→3,1→4)-β-glucan endohydrolases (β-glucanases; EI and EII) are primarily responsible for hydrolyzing high molecular weight (1→3,1→4)-β-glucans (β-glucan) during germination. Incomplete endosperm modification during malting results in residual β-glucan that can contribute to increased wort viscosity and beer chill haze. Four newly identified forms of EI and EII and the reference enzymes EI-a and EII-a were expressed in Escherichia coli, and the recombinant proteins were characterized for enzyme kinetics and thermostability...
January 18, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27896786/construction-of-efficient-streptococcus-zooepidemicus-strains-for-hyaluoronic-acid-production-based-on-identification-of-key-genes-involved-in-sucrose-metabolism
#19
Xuzhen Zhang, Man Wang, Tuanjie Li, Lixia Fu, Wei Cao, Hao Liu
Biosynthesis of polysaccharide hyaluoronic acid (HA) by Streptococcus zooepidemicus is a carbon-intensive process. The carbon flux and factor(s) restricting HA yield were not well understood. Here, we investigated the function of genes involved in sucrose metabolism and identified targets limiting HA yield, which were exploited to construct efficient S. zooepidemicus strains for HA production. The sucrose uptake was addressed by deletion of scrA and scrB, which encodes sucrose-PTS permease and sucrose-6-phosphate hydrolase, respectively...
December 2016: AMB Express
https://www.readbyqxmd.com/read/27862457/a-pts-eii-mutant-library-in-group-a-streptococcus-identifies-a-promiscuous-man-family-pts-transporter-influencing-sls-mediated-hemolysis
#20
Ganesh S Sundar, Emrul Islam, Kanika Gera, Yoann Le Breton, Kevin S McIver
The Group A Streptococcus (GAS, Streptococcus pyogenes) is a Gram-positive human pathogen that must adapt to unique host environments in order to survive. Links between sugar metabolism and virulence have been demonstrated in GAS, where mutants in the phosphoenolpyruvate-dependent phosphotransferase system (PTS) exhibited Streptolysin S (SLS)-mediated hemolysis during exponential growth. This early onset hemolysis correlated with an increased lesion size and severity in a murine soft tissue infection model when compared with parental M1T1 MGAS5005...
February 2017: Molecular Microbiology
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