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Massimo Busin

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https://www.readbyqxmd.com/read/27749446/tectonic-descemet-stripping-automated-endothelial-keratoplasty-for-the-management-of-sterile-corneal-perforations-in-decompensated-corneas
#1
Yoav Nahum, Irit Bahar, Massimo Busin
PURPOSE: To report the use of Descemet stripping automated endothelial keratoplasty (DSAEK) for treatment of sterile corneal perforations accompanying endothelial decompensation. METHODS: In this multicenter interventional case series, we reviewed the medical records of all tectonic DSAEK surgeries performed at Villa Serena-Villa Igea private Hospitals (Forlì, Italy) and Rabin Medical Center (Petach Tikva, Israel) between January 2014 and March 2016. RESULTS: Three patients with endothelial decompensation and sterile corneal perforation (n = 2) or impending corneal perforation (n = 1) underwent DSAEK between 2014 and 2015 at Villa Igea Hospital, Forlì, Italy, and Rabin Medical Center, Petach Tikva, Israel...
October 5, 2016: Cornea
https://www.readbyqxmd.com/read/27665215/corneal-epithelial-stem-cells-repopulate-the-donor-area-within-1-year-from-limbus-removal-for-limbal-autograft
#2
Massimo Busin, Claudia Breda, Marina Bertolin, Cristina Bovone, Diego Ponzin, Stefano Ferrari, Vanessa Barbaro, Hossein Mostafa Elbadawy
PURPOSE: To determine whether limbal epithelial stem cells (LESCs) repopulate the site harvested for limbal autograft transplantation (LAT), the expression of LESCs markers was evaluated in bioptic specimens obtained from the donor area 12 months or more after surgery. DESIGN: Interventional case series. PARTICIPANTS: Patients who underwent LAT for unilateral acquired limbal stem cell deficiency after chemical burn. METHODS: Corneal limbal explants were obtained from 2 sites, the harvested area and the untouched control area, in the donor eyes of 6 patients who previously underwent LAT for unilateral acquired limbal stem cell deficiency after chemical burn...
September 21, 2016: Ophthalmology
https://www.readbyqxmd.com/read/27149535/graft-recipient-collagen-lamellar-axis-discrepancy-is-compatible-with-excellent-visual-acuity-after-descemet-stripping-automated-endothelial-keratoplasty
#3
Yoav Nahum, Alfonso Iovieno, Eugenio Lipari, Luigi Fonatana, Massimo Busin
PURPOSE: To evaluate whether a discrepancy between the orientation of graft and recipient collagen lamellae can significantly limit the visual acuity after Descemet stripping automated endothelial keratoplasty (DSAEK). METHODS: A multicenter cohort study of eyes that underwent DSAEK for Fuchs endothelial dystrophy or pseudophakic bullous keratopathy at Villa Serena-Villa Igea private Hospitals (Forlì, Italy) and at Santa Maria Nuova public hospital (Reggio Emilia, Italy), which were scheduled for a postoperative examination between July and September 2014...
July 2016: Cornea
https://www.readbyqxmd.com/read/27066719/preloaded-tissues-for-descemet-membrane-endothelial-keratoplasty
#4
Mohit Parekh, Alessandro Ruzza, Stefano Ferrari, Massimo Busin, Diego Ponzin
INTRODUCTION: To determine the feasibility of preloading endothelial tissues for Descemet membrane endothelial keratoplasty (DMEK). DESIGN: Laboratory investigation. METHODS: setting: Institutional. PARTICIPANTS: Twenty human donor corneas unsuitable for transplantation with endothelial cell density in a range of 1600-2700 cells/mm(2). INTERVENTION: The endothelium was punched, stripped (8.5 mm diameter) and manually tri-folded with the endothelial side inward...
June 2016: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/27050357/two-cases-of-ultrathin-descemet-stripping-automated-endothelial-keratoplasty-utilizing-a-graft-that-had-undergone-radial-keratotomy
#5
Yoav Nahum, Diego Ponzin, Massimo Busin
This is a report of two cases in which tissue that had undergone radial keratotomy (RK) was utilized for double-pass ultrathin Descemet stripping automated endothelial keratoplasty (UT-DSAEK). Postoperative slit-lamp examination, visual acuity, anterior segment optical coherence tomography, and specular microscopy were available 30 months after surgery. Both corneas from a donor, who had undergone RK several years before his demise, and were otherwise suitable for endothelial keratoplasty were prepared for UT-DSAEK using double-pass dissection using first a 300 mm microkeratome head and then a 130μm microkeratome head (ALTK system, Moria, Antony, France)...
February 2016: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/26989960/visual-outcomes-of-repeat-versus-primary-descemet-stripping-automated-endothelial-keratoplasty-a-paired-comparison
#6
Yoav Nahum, Michael Mimouni, Silvana Madi, Massimo Busin
PURPOSE: To compare the results of repeat Descemet stripping automated endothelial keratoplasty (DSAEK) with those of primary DSAEK. MATERIALS AND METHODS: This retrospective study consisted of all consecutive eyes that underwent one or more repeat DSAEK surgeries after primary DSAEK performed at Villa Serena-Villa Igea Private Hospitals (Forlì, Italy) between January 2005 and June 2015. A paired comparison between the outcomes of primary and the second DSAEK was performed...
May 2016: Cornea
https://www.readbyqxmd.com/read/26863499/polarimetric-interferometry-for-assessment-of-corneal-stromal-lamellae-orientation
#7
Alfonso Iovieno, Sonia N Yeung, Yoav Nahum, Joshua Teichman, Eugenio Lipari, Massimo Busin, Luigi Fontana
PURPOSE: To analyze corneal stromal lamellae orientation in normal subjects and patients with various corneal conditions using a polarimetric interferometer. METHODS: Thirty-two healthy control subjects (59 eyes) and 16 patients (22 eyes) with corneal conditions or postkeratoplasty were included in the study. All patients were imaged using the interferometer and slit lamp photography. The interferometer produces an orthogonal cross-like image of stromal lamellae by illuminating the cornea in phase-light polarization angle...
April 2016: Cornea
https://www.readbyqxmd.com/read/26772875/outcomes-of-air-injection-within-2%C3%A2-mm-inside-a-deep-trephination-for-deep-anterior-lamellar-keratoplasty-in-eyes-with-keratoconus
#8
Massimo Busin, Vincenzo Scorcia, Pia Leon, Yoav Nahum
PURPOSE: To evaluate the outcomes of a new technique for deep anterior lamellar keratoplasty (DALK) employing the injection of air up to 2 mm inside a deep trephination (intended within 100 μm from the endothelial surface) obtained with a guarded trephine set by means of anterior segment optical coherence tomography (AS OCT). DESIGN: Retrospective, noncomparative, interventional case series. METHODS: The success rate and learning curve of pneumatic dissection in one clinical practice were analyzed in nonscarred keratoconic eyes undergoing a standardized DALK including 9-mm trephination intended to a depth within 100 μm from the endothelial surface, based on the thinnest AS OCT measurement at this site; and injection of air through a cannula advanced 1-2 mm centripetally from the bottom of the trephination...
April 2016: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/26686969/contact-lens-assisted-pull-through-technique-for-delivery-of-tri-folded-endothelium-in-dmek-grafts-minimizes-surgical-time-and-cell-loss
#9
Massimo Busin, Pia Leon, Vincenzo Scorcia, Diego Ponzin
PURPOSE: To evaluate the feasibility and outcomes of contact lens-assisted bimanual pull-through delivery of Descemet membrane endothelial keratoplasty (DMEK) tissue trifolded with the endothelium inward. DESIGN: Prospective, noncomparative, interventional case series. PARTICIPANTS: Forty-two consecutive eyes of 42 patients with Fuchs endothelial dystrophy with or without cataract. INTERVENTION: Standardized DMEK was performed as a single procedure (n = 9) or in combination with phacoemulsification and implantation of a posterior chamber intraocular lens (n = 33) using prestripped donor tissue punched to a diameter of 8...
March 2016: Ophthalmology
https://www.readbyqxmd.com/read/26655486/results-of-descemet-stripping-automated-endothelial-keratoplasty-for-the-treatment-of-late-corneal-decompensation-secondary-to-obstetrical-forceps-trauma
#10
Vincenzo Scorcia, Rocco Pietropaolo, Adriano Carnevali, Valentina De Luca, Andrea Lucisano, Massimo Busin
PURPOSE: To describe the clinical outcomes of Descemet stripping automated endothelial keratoplasty (DSAEK) in a series of patients affected by progressive endothelial cell loss leading to corneal decompensation with Descemet membrane (DM) breaks caused by obstetrical forceps trauma. METHODS: Seven amblyopic eyes of 7 patients affected by unilateral visual loss due to increasing corneal edema were included in the study. In all eyes, slit-lamp examination revealed typical DM breaks and diffuse corneal edema: these findings were compatible with a history of obstetrical forceps-assisted delivery...
March 2016: Cornea
https://www.readbyqxmd.com/read/26538771/a-two-piece-microkeratome-assisted-mushroom-keratoplasty-improves-the-outcomes-and-survival-of-grafts-performed-in-eyes-with-diseased-stroma-and-healthy-endothelium-an-american-ophthalmological-society-thesis
#11
Massimo Busin, Silvana Madi, Vincenzo Scorcia, Paolo Santorum, Yoav Nahum
PURPOSE: To test the hypothesis that a new microkeratome-assisted penetrating keratoplasty (PK) technique employing transplantation of a two-piece mushroom-shaped graft may result in better visual outcomes and graft survival rates than those of conventional PK. METHODS: Retrospective chart review of 96 eyes at low risk and 76 eyes at high risk for immunologic rejection (all with full-thickness central corneal opacity and otherwise healthy endothelium) undergoing mushroom PK between 2004 and 2012 at our Institution...
2015: Transactions of the American Ophthalmological Society
https://www.readbyqxmd.com/read/26356756/postoperative-graft-thickness-obtained-with-single-pass-microkeratome-assisted-ultrathin-descemet-stripping-automated-endothelial-keratoplasty
#12
Yoav Nahum, Pia Leon, Massimo Busin
PURPOSE: To evaluate the results of microkeratome-assisted ultrathin Descemet stripping automated endothelial keratoplasty (UT-DSAEK) using a nomogram developed for the single-pass technique. METHODS: A retrospective study of eyes operated between September 2014 and March 2015 at Villa Serena-Villa Igea Private Hospitals (Forlì, Italy) by a single surgeon (M.B.). Study eyes underwent UT-DSAEK performed using a linear microkeratome-assisted lamellar dissection with disposable heads of different sizes (Moria ALTK system; Moria SA) chosen according to a pachymetry-based nomogram developed at our department...
November 2015: Cornea
https://www.readbyqxmd.com/read/26253099/descemet-stripping-automated-endothelial-keratoplasty-in-fuchs-corneal-endothelial-dystrophy-anterior-segment-optical-coherence-tomography-and-in-vivo-confocal-microscopy-analysis
#13
Rita Mencucci, Eleonora Favuzza, Ruggero Tartaro, Massimo Busin, Gianni Virgili
BACKGROUND: To evaluate the in vivo corneal changes using in vivo confocal microscopy (IVCM) and anterior segment optical coherence tomography (AS-OCT) in patients with Fuchs' dystrophy who underwent Descemet stripping automated endothelial keratoplasty (DSAEK) and the relationship between these changes and the postoperative visual recovery up to 1-year follow-up. METHODS: Before DSAEK and 1 day, 3, 6 and 12 months after surgery 31 patients (39 pseudophakic eyes) underwent a complete ophthalmological evaluation including best corrected visual acuity (BCVA), IVCM (subepithelial haze, interface haze, graft thickness) and AS-OCT (graft thickness)...
2015: BMC Ophthalmology
https://www.readbyqxmd.com/read/26203751/red-reflex-guided-big-bubble-deep-anterior-lamellar-keratoplasty-a-simple-technique-to-judge-dissection-depth
#14
Vincenzo Scorcia, Andrea Lucisano, Rocco Pietropaolo, Vincenzo Savoca Corona, Giovanni Scorcia, Massimo Busin
PURPOSE: To report the results of a modified big-bubble deep anterior lamellar keratoplasty technique using the intraoperative red reflex to visualize the amount of residual stroma beneath the inserted cannula. METHODS: A total of 132 consecutive keratoconic eyes were included in this retrospective, noncomparative, interventional case series. Before starting surgery, pharmacologic mydriasis was induced in all eyes undergoing a big-bubble deep anterior lamellar keratoplasty procedure...
September 2015: Cornea
https://www.readbyqxmd.com/read/26020823/risk-factors-predicting-the-need-for-graft-exchange-after-descemet-stripping-automated-endothelial-keratoplasty
#15
Yoav Nahum, Michael Mimouni, Massimo Busin
PURPOSE: To identify the risk factors predicting the need for graft exchange after Descemet stripping automated endothelial keratoplasty (DSAEK). METHODS: This is a retrospective cohort study. The study group included all 117 eyes that underwent or necessitated repeat DSAEK performed between January 2005 and June 2014 at Villa Serena-Villa Igea private hospitals (Forlì, Italy). The control group consisted of 1033 eyes that underwent only primary DSAEK during the same period...
August 2015: Cornea
https://www.readbyqxmd.com/read/25957376/prevalence-of-guttae-in-the-graft-following-corneal-transplantation
#16
Yoav Nahum, Veronica Canton, Diego Ponzin, Massimo Busin
AIM: To evaluate the prevalence of guttae in donor grafts following corneal transplantation and to examine the possible effect of guttae on postoperative results. METHODS: Retrospective cohort study. We reviewed the medical records of all keratoplasties performed at the Villa Serena-Villa Igea private hospitals (Forlì, Italy) between January 2005 and July 2014. Endothelial specular microscopy images were examined to identify the presence of guttae. Donor's age, patient's age, indication for surgery, surgical procedure, postoperative visual acuity, and endothelial cell density were also noted...
December 2015: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/25926517/preloaded-donor-corneal-lenticules-in-a-new-validated-3d-printed-smart-storage-glide-for-descemet-stripping-automated-endothelial-keratoplasty
#17
Alessandro Ruzza, Mohit Parekh, Stefano Ferrari, Gianni Salvalaio, Yoav Nahum, Cristina Bovone, Diego Ponzin, Massimo Busin
PURPOSE: To design and validate the efficacy of three-dimensional (3D) printed smart storage glide (SSG) which is capable of preserving and delivering posterior lenticules for Descemet stripping automated endothelial keratoplasty (DSAEK). METHODS: Laboratory investigation (A) was followed by clinical validation (B). Unsuitable corneas for transplantation (n=20) were used for study A. These tissues were cut using a standard microkeratome, punched and loaded into the SSG and preserved for 7 days in transport media...
October 2015: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/25606709/surgical-technique-for-graft-exchange-after-big-bubble-deep-anterior-lamellar-keratoplasty
#18
Vincenzo Scorcia, Jacqueline Beltz, Andrea Lucisano, Giovanni Scorcia, Massimo Busin
PURPOSE: The aim of this study was to describe a surgical technique for repeat deep anterior lamellar keratoplasty (DALK) by baring Descemet membrane again in eyes affected by stromal opacity of the donor lamella. METHODS: Repeat DALK was performed in 5 eyes of 5 patients affected by central stromal opacity not involving the endothelium; indications for repeat surgery were postbacterial or postherpetic corneal scars (n = 3), postphotorefractive keratectomy haze (n = 1), and recurrence of granular dystrophy (n = 1)...
April 2015: Cornea
https://www.readbyqxmd.com/read/25564336/ic3d-classification-of-corneal-dystrophies-edition-2
#19
Jayne S Weiss, Hans Ulrik Møller, Anthony J Aldave, Berthold Seitz, Cecilie Bredrup, Tero Kivelä, Francis L Munier, Christopher J Rapuano, Kanwal K Nischal, Eung Kweon Kim, John Sutphin, Massimo Busin, Antoine Labbé, Kenneth R Kenyon, Shigeru Kinoshita, Walter Lisch
PURPOSE: To update the 2008 International Classification of Corneal Dystrophies (IC3D) incorporating new clinical, histopathologic, and genetic information. METHODS: The IC3D reviewed worldwide peer-reviewed articles for new information on corneal dystrophies published between 2008 and 2014. Using this information, corneal dystrophy templates and anatomic classification were updated. New clinical, histopathologic, and confocal photographs were added. RESULTS: On the basis of revisiting the cellular origin of corneal dystrophy, a modified anatomic classification is proposed consisting of (1) epithelial and subepithelial dystrophies, (2) epithelial-stromal TGFBI dystrophies, (3) stromal dystrophies, and (4) endothelial dystrophies...
February 2015: Cornea
https://www.readbyqxmd.com/read/25411935/inadvertent-donor-button-inversion-during-big-bubble-deep-anterior-lamellar-keratoplasty
#20
Vincenzo Scorcia, Franco D Cosimo, Andrea Lucisano, Diego Ponzin, Giovanni Scorcia, Massimo Busin
PURPOSE: The aim of this study was to describe clinical outcomes and histopathologic findings in a case of repeat deep anterior lamellar keratoplasty (DALK) performed because of inadvertent inversion of the donor button at the time of primary surgery. METHODS: A 34-year-old woman underwent big-bubble DALK for keratoconus in her right eye; 4 days postoperatively, slit-lamp examination revealed the presence of several inclusions in the interface, whereas anterior segment optical coherence tomography (AS-OCT) showed pathologically marked wrinkling of the posterior stroma; inadvertent intraoperative inversion of the graft was diagnosed and the interface inclusions were assumed to be of epithelial origin...
January 2015: Cornea
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