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https://www.readbyqxmd.com/read/28639116/integration-of-transcriptomic-and-metabolic-data-reveals-hub-transcription-factors-involved-in-drought-stress-response-in-sunflower-helianthus-annuus-l
#1
Sebastián Moschen, Julio A Di Rienzo, Janet Higgins, Takayuki Tohge, Mutsumi Watanabe, Sergio González, Máximo Rivarola, Francisco García-García, Joaquin Dopazo, H Esteban Hopp, Rainer Hoefgen, Alisdair R Fernie, Norma Paniego, Paula Fernández, Ruth A Heinz
By integration of transcriptional and metabolic profiles we identified pathways and hubs transcription factors regulated during drought conditions in sunflower, useful for applications in molecular and/or biotechnological breeding. Drought is one of the most important environmental stresses that effects crop productivity in many agricultural regions. Sunflower is tolerant to drought conditions but the mechanisms involved in this tolerance remain unclear at the molecular level. The aim of this study was to characterize and integrate transcriptional and metabolic pathways related to drought stress in sunflower plants, by using a system biology approach...
June 21, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28634826/tai-vacuolar-invertase-orthologs-the-interspecific-variability-in-tomato-plants-solanum-section-lycopersicon
#2
M A Slugina, A V Shchennikova, E Z Kochieva
Understanding the genetic mechanisms underlying carbohydrate metabolism can promote the development of biotechnological advances in fruit plants. The flesh tomato fruit represents an ideal system for examining the role of sucrose cleavage enzymes in fruit development, and wild tomato species differing in storage sugars serve as an excellent research material for this purpose. Plant vacuolar invertase is a key enzyme of sucrose metabolism in the sink organs. In the present study, we identified complete gene sequences encoding the TAI vacuolar invertase in 11 wild and one cultivated tomato accessions of the Solanum section Lycopersicon...
June 20, 2017: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/28634484/manipulation-of-metabolic-pathways-to-develop-vitamin-enriched-crops-for-human-health
#3
REVIEW
Ling Jiang, Weixuan Wang, Tong Lian, Chunyi Zhang
Vitamin deficiencies are major forms of micronutrient deficiencies, and are associated with huge economic losses as well as severe physical and intellectual damages to humans. Much evidence has demonstrated that biofortification plays an important role in combating vitamin deficiencies due to its economical and effective delivery of nutrients to populations in need. Biofortification enables food plants to be enriched with vitamins through conventional breeding and/or biotechnology. Here, we focus on the progress in the manipulation of the vitamin metabolism, an essential part of biofortification, by the genetic modification or by the marker-assisted selection to understand mechanisms underlying metabolic improvement in food plants...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28633085/biotechnological-production-of-recombinant-tissue-plasminogen-activator-protein-reteplase-from-transplastomic-tobacco-cell-cultures
#4
Diego Hidalgo, Maryam Abdoli-Nasab, Mokhtar Jalali-Javaran, Roque Bru-Martínez, Rosa M Cusidó, Purificación Corchete, Javier Palazon
Transplastomic plants are a system of choice for the mass production of biopharmaceuticals due to the polyploidy of the plastid genome and the low risk of pollen-mediated outcrossing because of maternal inheritance. However, as field-grown plants, they can suffer contamination by agrochemicals and fertilizers, as well as fluctuations in yield due to climatic changes and infections. Tissue-type plasminogen activator (tPA), a protein used to treat heart attacks, converts plasminogen into plasmine, which digests fibrin and induces the dissolution of fibrin clots...
June 13, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28632186/insights-into-penicillium-brasilianum-secondary-metabolism-and-its-biotechnological-potential
#5
REVIEW
Jaqueline Moraes Bazioli, Luciana Da Silva Amaral, Taícia Pacheco Fill, Edson Rodrigues-Filho
Over the past few years Penicillium brasilianum has been isolated from many different environmental sources as soil isolates, plant endophytes and onion pathogen. All investigated strains share a great ability to produce bioactive secondary metabolites. Different authors have investigated this great capability and here we summarize the metabolic potential and the biological activities related to P. brasilianum's metabolites with diverse structures. They include secondary metabolites of an alkaloid nature, i...
June 20, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28630123/absence-of-the-polar-organizing-protein-popz-causes-aberrant-cell-division-in-agrobacterium-tumefaciens
#6
Matthew Howell, Alena Aliashkevich, Anne K Salisbury, Felipe Cava, Grant R Bowman, Pamela J B Brown
Agrobacterium tumefaciens is a rod-shaped bacterium that grows by polar insertion of new peptidoglycan during cell elongation. As the cell cycle progresses, peptidoglycan synthesis at the pole ceases prior to insertion of new peptidoglycan at mid-cell to enable cell division. The A. tumefaciens homolog of the Caulobacter crescentus polar organelle development protein PopZ has been identified as a growth pole marker and a candidate polar growth promoting factor. Here, we characterize the function of PopZ in cell growth and division of A...
June 19, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28629185/leptadenia-reticulata-retz-wight-arn-jivanti-botanical-agronomical-phytochemical-pharmacological-and-biotechnological-aspects
#7
REVIEW
Sudipta Kumar Mohanty, Mallappa Kumara Swamy, Uma Rani Sinniah, Maniyam Anuradha
Leptadenia reticulata (Retz.) Wight & Arn. (Apocynaceae), is a traditional medicinal plant species widely used to treat various ailments such as tuberculosis, hematopoiesis, emaciation, cough, dyspnea, fever, burning sensation, night blindness, cancer, and dysentery. In Ayurveda, it is known for its revitalizing, rejuvenating, and lactogenic properties. This plant is one of the major ingredients in many commercial herbal formulations, including Speman, Envirocare, Calshakti, Antisept, and Chyawanprash. The therapeutic potential of this herb is because of the presence of diverse bioactive compounds such as α-amyrin, β-amyrin, ferulic acid, luteolin, diosmetin, rutin, β-sitosterol, stigmasterol, hentricontanol, a triterpene alcohol simiarenol, apigenin, reticulin, deniculatin, and leptaculatin...
June 19, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28623735/biochemical-and-structural-characterization-of-penicillium-purpurogenum-%C3%AE-d-galactosidase-binding-of-galactose-to-an-alternative-pocket-may-explain-enzyme-inhibition
#8
Luis Morales-Quintana, Carolina Faúndez, Raúl Herrera, Vasni Zavaleta, María Cristina Ravanal, Jaime Eyzaguirre, María Alejandra Moya-León
The fungus Penicillium purpurogenum degrades plant cell walls by the action of cellulolytic, xylanolytic and pectinolytic enzymes. The α-D-galactosidase is one of the enzymes which may act on pectin degradation. This enzyme has several biotechnological and medical applications. The aim of this work was to better understand the molecular mechanism of α-D-galactosidase from P. purpurogenum (GALP1). For this purpose, a gene coding for the enzyme was identified from the fungal genome and heterologously expressed in Pichia pastoris...
June 9, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28623383/ethnopharmacological-uses-phytochemistry-biological-activities-and-biotechnological-applications-of-eclipta-prostrata
#9
REVIEW
Ill-Min Chung, Govindasamy Rajakumar, Ji-Hee Lee, Seung-Hyun Kim, Muthu Thiruvengadam
Eclipta prostrata belongs to a family of medicinal plants (Asteraceae) and plays a role in the treatment of several diseases, including infectious hepatitis, snake venom poisoning, gastritis, and respiratory diseases such as a cough and asthma. A number of compounds, including thiophene derivatives, steroids, triterpenes, flavonoids, polyacetylenes, polypeptides, and coumestans, have been isolated from E. prostrata. The plant functional compounds can act as reducing agent in the field of nanoparticle synthesis...
June 16, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28617654/function-discovery-and-exploitation-of-plant-pattern-recognition-receptors-for-broad-spectrum-disease-resistance
#10
Freddy Boutrot, Cyril Zipfel
Plants are constantly exposed to would-be pathogens and pests, and thus have a sophisticated immune system to ward off these threats, which otherwise can have devastating ecological and economic consequences on ecosystems and agriculture. Plants employ receptor kinases (RKs) and receptor-like proteins (RLPs) as pattern recognition receptors (PRRs) to monitor their apoplastic environment and detect non-self and damaged-self patterns as signs of potential danger. Plant PRRs contribute to both basal and non-host resistances, and treatment with pathogen-/microbe-associated molecular patterns (PAMPs/MAMPs) or damage-associated molecular patterns (DAMPs) recognized by plant PRRs induces both local and systemic immunity...
June 15, 2017: Annual Review of Phytopathology
https://www.readbyqxmd.com/read/28612104/production-of-paclitaxel-with-anticancer-activity-by-two-local-fungal-endophytes-aspergillus-fumigatus-and-alternaria-tenuissima
#11
Ahmed A Ismaiel, Ashraf S Ahmed, Ismail A Hassan, El-Sayed R El-Sayed, Al-Zahraa A Karam El-Din
Among 60 fungal endophytes isolated from twigs, bark, and mature leaves of different plant species, two fungal isolates named TXD105 and TER995 were capable of producing paclitaxel in amounts of up to 84.41 and 37.92 μg L(-1), respectively. Based on macroscopic and microscopic characteristics, ITS1-5.8S-ITS2 rDNA sequence, and phylogenetic characteristic analysis, the two respective isolates were identified as Aspergillus fumigatus and Alternaria tenuissima. In the effort to increase paclitaxel magnitude by the two fungal strains, several fermentation conditions including selection of the proper fermentation medium, agitation rate, incubation temperature, fermentation period, medium pH, medium volume, and inoculum nature (size and age of inoculum) were tried...
June 13, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28611737/de-novo-biosynthesis-of-apigenin-luteolin-and-eriodictyol-in-the-actinomycete-streptomyces-albus-and-production-improvement-by-feeding-and-spore-conditioning
#12
Laura Marín, Ignacio Gutiérrez-Del-Río, Paula Yagüe, Ángel Manteca, Claudio J Villar, Felipe Lombó
Nutraceutical compounds as plant flavonoids play an important role in prevention and modulation of diverse heath conditions, as they exert interesting antifungal, antibacterial, antioxidant, and antitumor effects. They also possess anti-inflammatory activities in arthritis, cardiovascular disease or neurological diseases, as well as modulatory effects on the CYP450 activity on diverse drugs. Most flavonoids are bioactive molecules of plant origin, but their industrial production is sometimes hindered due to reasons as low concentration in the plant tissues, presence in only some species or as a complex mixture or inactive glycosides in plant vacuolae...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28610997/identification-of-multienzymatic-complexes-in-the-clonostachys-byssicola-secretomes-produced-in-response-to-different-lignocellulosic-carbon-sources
#13
Helder Andrey Rocha Gomes, Adelson Joel da Silva, Diana Paola Gómez-Mendoza, Agenor Castro Moreira Dos Santos Júnior, Nicholas de Mojana di Cologna, Rosane Mansan Almeida, Robert Neil Gerard Miller, Wagner Fontes, Marcelo Valle de Sousa, Carlos André Ornelas Ricart, Edivaldo Ximenes Ferreira Filho
Multienzymatic complexes with plant lignocellulose-degrading activities have recently been identified in filamentous fungi secretomes. Such complexes have potential biotechnological applications in the degradation of agro-industrial residues. Fungal species from the Clonostachys genus have been intensively investigated as biocontrol agents; however so far their use as producers of lignocellulose-degrading enzymes has not been extensively explored. Secretomes of Clonostachys byssicola following growth on different carbon sources (passion fruit peel, soybean hulls, cotton gin trash, banana stalk, sugarcane bagasse, orange peel, and a composition of soybean hulls: cotton gin trash:orange peel) were subjected to enzymatic assays...
June 10, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28608823/production-of-putative-diterpene-carboxylic-acid-intermediates-of-triptolide-in-yeast
#14
Victor Forman, Roberta Callari, Christophe Folly, Harald Heider, Björn Hamberger
The development of medical applications exploiting the broad bioactivities of the diterpene therapeutic triptolide from Tripterygium wilfordii is limited by low extraction yields from the native plant. Furthermore, the extraordinarily high structural complexity prevents an economically attractive enantioselective total synthesis. An alternative production route of triptolide through engineered Saccharomyces cerevisiae (yeast) could provide a sustainable source of triptolide. A potential intermediate in the unknown biosynthetic route to triptolide is the diterpene dehydroabietic acid...
June 13, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28606405/biotechnological-advances-for-restoring-degraded-land-for-sustainable-development
#15
REVIEW
Vishal Tripathi, Sheikh Adil Edrisi, Bin Chen, Vijai K Gupta, Raivo Vilu, Nicholas Gathergood, P C Abhilash
Global land resources are under severe threat due to pollution and unsustainable land use practices. Restoring degraded land is imperative for regaining ecosystem services, such as biodiversity maintenance and nutrient and water cycling, and to meet the food, feed, fuel, and fibre requirements of present and future generations. While bioremediation is acknowledged as a promising technology for restoring polluted and degraded lands, its field potential is limited for various reasons. However, recent biotechnological advancements, including producing efficient microbial consortia, applying enzymes with higher degrees of specificity, and designing plants with specific microbial partners, are opening new prospects in remediation technology...
June 9, 2017: Trends in Biotechnology
https://www.readbyqxmd.com/read/28597218/uncovering-a-new-moral-dilemma-of-economic-optimization-in-biotechnological-processing
#16
Marek Vochozka, Vojtěch Stehel, Anna Maroušková
The trend of emerging biorefineries is to process the harvest as efficiently as possible and without any waste. From the most valuable phytomass, refined medicines, enzymes, dyes and other special reactants are created. Functional foods, food ingredients, oils, alcohol, solvents, plastics, fillers and a wide variety of other chemical products follow. After being treated with nutrient recovery techniques (for fertilizer production), biofuels or soil improvers are produced from the leftovers. Economic optimization algorithms have confirmed that such complex biorefineries can be financially viable only when a high degree of feedstock concentration is included...
June 8, 2017: Science and Engineering Ethics
https://www.readbyqxmd.com/read/28595545/anticancer-polyphenols-from-cultured-plant-cells-production-and-new-bioengineering-strategies
#17
Victor Bulgakov, Yulia V Vereshchagina, Galina N Veremeichik
For many years, anticancer polyphenols have attracted significant attention as substances that prevent tumor growth and progression. These compounds are simple phenolic acids; complex phenolic acids, such as caffeoylquinic acids, rosmarinic acid and its derivatives; stilbenes (resveratrol and piceatannol); flavones; isoflavones (genistein and tectorigenin); and anthocyanins. Some compounds, such as tea and coffee polyphenols, can be produced in large quantities by traditional methods, while many others cannot...
June 8, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28588643/molecular-cloning-expression-and-characterization-of-four-novel-thermo-alkaliphilic-enzymes-retrieved-from-a-metagenomic-library
#18
Mukil Maruthamuthu, Jan Dirk van Elsas
BACKGROUND: Enzyme discovery is a promising approach to aid in the deconstruction of recalcitrant plant biomass in an industrial process. Novel enzymes can be readily discovered by applying metagenomics on whole microbiomes. Our goal was to select, examine, and characterize eight novel glycoside hydrolases that were previously detected in metagenomic libraries, to serve biotechnological applications with high performance. RESULTS: Here, eight glycosyl hydrolase family candidate genes were selected from metagenomes of wheat straw-degrading microbial consortia using molecular cloning and subsequent gene expression studies in Escherichia coli...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28584123/characterization-of-the-macrocyclase-involved-in-the-biosynthesis-of-ripp-cyclic-peptides-in-plants
#19
Jonathan R Chekan, Paola Estrada, Patrick S Covello, Satish K Nair
Enzymes that can catalyze the macrocyclization of linear peptide substrates have long been sought for the production of libraries of structurally diverse scaffolds via combinatorial gene assembly as well as to afford rapid in vivo screening methods. Orbitides are plant ribosomally synthesized and posttranslationally modified peptides (RiPPs) of various sizes and topologies, several of which are shown to be biologically active. The diversity in size and sequence of orbitides suggests that the corresponding macrocyclases may be ideal catalysts for production of cyclic peptides...
June 20, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28583179/comparison-between-the-effects-of-potassium-phosphite-and-chitosan-on-changes-in-the-concentration-of-cucurbitacin-e-and-on-antibacterial-property-of-cucumis-sativus
#20
Moazzameh Ramezani, Fatemeh Rahmani, Ali Dehestani
BACKGROUND: Cucurbitacins are mostly found in the members of the family Cucurbitaceae and are responsible for the bitter taste of cucumber. Pharmacological activities such as anti-bacterial and anti-tumor effects have been attributed to these structurally divers triterpens. The aim of this study was to investigate the effect of potassium phosphite (KPhi) and chitosan on Cucurbitacin E (CuE) concentration in different tissues of Cucumis sativus. The antibacterial effect of plant ethanolic extracts was also examined against E...
June 5, 2017: BMC Complementary and Alternative Medicine
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