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https://www.readbyqxmd.com/read/28738232/molecular-tools-and-emerging-strategies-for-deep-genetic-genomic-refactoring-of-pseudomonas
#1
REVIEW
Esteban Martínez-García, Víctor de Lorenzo
The interest of the genus Pseudomonas largely relies on the virulence of some of its species for plants and animals (including humans). Yet, pathogenic features of some isolates coexist with others often present in environmental variants that promote plant growth and degrade chemical pollutants. Many of these traits can be traced to the intrinsic properties of the genomic chassis of this genus along with distinct genetic parts and devices. With the tools of Synthetic Biology these can be enhanced and/or repurposed for the sake of biological control, environmental remediation and whole-cell biocatalysis...
July 21, 2017: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/28736573/a-rapid-and-efficient-in-vitro-regeneration-system-for-lettuce-lactuca-sativa-l
#2
Isabel Armas, Natalia Pogrebnyak, Ilya Raskin
BACKGROUND: Successful biotechnological improvement of crop plants requires a reliable and efficient in vitro regeneration system. Lettuce (Lactuca sativa L.), one the most important vegetable crops worldwide, is strongly genotype-dependent in terms of regeneration capacity, limiting the potential for biotechnological improvement of cultivars which show recalcitrance under currently available protocols. The effect of different nutrient sources, plant hormone combinations and activated charcoal supplementation on shoot induction efficiency was evaluated on the cultivar 'RSL NFR', which had previously shown poor regeneration efficiency...
2017: Plant Methods
https://www.readbyqxmd.com/read/28726109/the-study-of-immunogenicity-of-the-antigenic-protein-of-high-risk-oncogenic-type-of-the-human-papillomavirus-hpv16-l1-produced-in-the-plant-expression-system-on-the-base-of-transgenic-tomato
#3
R K Salyaev, N I Rekoslavskaya, A S Stolbikov, A V Tretyakova
The immunogenicity of plant-made peroral vaccine against cervical cancer was studied in mice during 342 days after vaccination with the material of tomato fruits genetically transformed with HPV16 L1 Ώ 5'UTR TMV. The immune response was found on day 4 after vaccination in blood serum of vaccinated mice. On days 90-100, the rise to maximum value of the level of antibodies to HPV16 L1 was in the range of 500 ng of the standard antibodies to HPV16 L1 (Santa Cruz Biotechnology, United States). This level of antibodies was retained until the end of the study...
May 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28725265/two-bifunctional-enzymes-from-the-marine-protist-thraustochytrium-roseum-biochemical-characterization-of-wax-ester-synthase-acyl-coa-diacylglycerol-acyltransferase-activity-catalyzing-wax-ester-and-triacylglycerol-synthesis
#4
Nannan Zhang, Zejing Mao, Ling Luo, Xia Wan, Fenghong Huang, Yangmin Gong
BACKGROUND: Triacylglycerols (TAGs) and wax esters (WEs) are important neutral lipids which serve as energy reservoir in some plants and microorganisms. In recent years, these biologically produced neutral lipids have been regarded as potential alternative energy sources for biofuel production because of the increased interest on developing renewable and environmentally benign alternatives for fossil fuels. In bacteria, the final step in TAG and WE biosynthetic pathway is catalyzed by wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase (WS/DGAT)...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28724619/plant-glandular-trichomes-natural-cell-factories-of-high-biotechnological-interest
#5
Alexandre Huchelmann, Marc Boutry, Charles Hachez
No abstract text is available yet for this article.
July 19, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28721831/transient-production-of-recombinant-pharmaceutical-proteins-in-plants-evolution-and-perspectives
#6
Lilya Kopertekh, Joachim Schiemann
During the last two decades the production of pharmaceutical proteins in plants evolved from proof of concept to established technology adopted by several biotechnological companies. This progress is particularly based on intensive research starting stable genetic transformation and moving to transient expression. Due to its advantages in yield and speed of protein production transient expression platforms became leading plant-based manufacturing technology. Current transient expression methods rely on Agrobacterium-mediated delivery of expression vectors into plant cells...
July 18, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28712492/genome-engineering-and-agriculture-opportunities-and-challenges
#7
Nicholas J Baltes, Javier Gil-Humanes, Daniel F Voytas
In recent years, plant biotechnology has witnessed unprecedented technological change. Advances in high-throughput sequencing technologies have provided insight into the location and structure of functional elements within plant DNA. At the same time, improvements in genome engineering tools have enabled unprecedented control over genetic material. These technologies, combined with a growing understanding of plant systems biology, will irrevocably alter the way we create new crop varieties. As the first wave of genome-edited products emerge, we are just getting a glimpse of the immense opportunities the technology provides...
2017: Progress in Molecular Biology and Translational Science
https://www.readbyqxmd.com/read/28711981/universal-direct-pcr-amplification-system-a-time-and-cost-effective-tool-for-high-throughput-applications
#8
Anis Ben-Amar, Souheib Oueslati, Ahmed Mliki
Taking into account the limits of current genotyping methodologies, we have established a versatile direct PCR method on intact microtissue samples without prior DNA isolation. A simple and standard protocol was developed and validated on a wide range of living organisms including bacterial and fungal strains, plant species and human samples. This allows reliable amplification of target genomic DNA fragment directly from source material using minimal amount of tissue which makes DNA purification irrelevant for a number of biological applications...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28710428/the-use-of-a-hybrid-sequential-biofiltration-system-for-the-improvement-of-nutrient-removal-and-pcb-control-in-municipal-wastewater
#9
Edyta Kiedrzyńska, Magdalena Urbaniak, Marcin Kiedrzyński, Adam Jóźwik, Agnieszka Bednarek, Ilona Gągała, Maciej Zalewski
This article aims to evaluate the efficiency of an innovative hybrid Sequential Biofiltration System (SBS) for removing phosphorus and nitrogen and polychlorinated biphenyls (PCBs) from original municipal wastewater produced by a Wastewater Treatment Plant under authentic operating conditions. The hybrid SBS was constructed with two barriers, a geochemical (filtration beds with limestone, coal and sawdust) and a biological barrier (wetlands with Glyceria, Acorus, Typha, Phragmites), operating in parallel. Significant differences were found between inflow and outflow from the SBS with regard to wastewater contaminant concentrations, the efficiency of removal being 16% (max...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28707275/vanilla-vanilla-planifolia-jacks-cell-suspension-cultures-establishment-characterization-and-applications
#10
Marco A Ramírez-Mosqueda, Lourdes G Iglesias-Andreu
The establishment and characterization of cell suspension cultures are an in vitro culture technique very useful for various plant biotechnological applications (production of secondary metabolites, mass micropropagation, protoplast isolation and fusion, gene transfer and the investigation of cell pathways). The objective of this study was to establish and characterization of cell suspension cultures of V. planifolia by inducing friable calluses. For that, friable calluses were obtained from immature seeds cultivated in MS medium supplemented with 0...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28704927/the-tor-signaling-network-in-the-model-unicellular-green-alga-chlamydomonas-reinhardtii
#11
REVIEW
María Esther Pérez-Pérez, Inmaculada Couso, José L Crespo
Cell growth is tightly coupled to nutrient availability. The target of rapamycin (TOR) kinase transmits nutritional and environmental cues to the cellular growth machinery. TOR functions in two distinct multiprotein complexes, termed TOR complex 1 (TORC1) and TOR complex 2 (TORC2). While the structure and functions of TORC1 are highly conserved in all eukaryotes, including algae and plants, TORC2 core proteins seem to be missing in photosynthetic organisms. TORC1 controls cell growth by promoting anabolic processes, including protein synthesis and ribosome biogenesis, and inhibiting catabolic processes such as autophagy...
July 12, 2017: Biomolecules
https://www.readbyqxmd.com/read/28699508/edible-vaccine-current-status-and-future-perspectives
#12
Sayed Sartaj Sohrab, Mohd Suhail, Mohammad A Kamal, Azamal Husen, Esam I Azhar
Growing world population and continuous disease emergence have invited the development of more efficient new vaccines against a range of diseases. Conventional vaccines are being wildly used in the world but their production requires higher cost, more time and better infrastructure. Thus, the idea of plant-based edible vaccine technology has emerged and showed promising results with strong and effective protection against many diseases. Plants have been utilized since more than two decades as pharmaceuticals against many diseases...
July 11, 2017: Current Drug Metabolism
https://www.readbyqxmd.com/read/28696064/harnessing-microbiome-based-biotechnologies-for-sustainable-mitigation-of-nitrous-oxide-emissions
#13
Hang-Wei Hu, Ji-Zheng He, Brajesh K Singh
Achieving the Sustainable Development Goal of climate change mitigation within this century will require adoption of new innovative technologies to control emissions of nitrous oxide (N2 O), an important greenhouse gas leading to global warming. This is particularly important in the face of growing fertilizer consumption and continuous land degradation. Currently used tools to mitigate N2 O emissions are based on agrochemical inputs and agronomic practices. Emerging technologies include plant breeding approaches to manipulate microbiome activities in agro-ecosystems, and microbial biotechnology approaches for in situ microbiome manipulation and engineering via use of biochemical, cellular and genome-editing methods...
July 11, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28696041/the-contribution-of-microbial-biotechnology-to-sustainable-development-goals-microbiome-therapies
#14
Paul W O'Toole, Max Paoli
Complex communities of microbes live on and in plants, humans and other animals. These communities are collectively referred to as the microbiota or microbiome. Plants and animals evolved to co-exist with these microbes. In mammals, particular kinds of alteration of the microbiome (dysbiosis) are associated with loss of health, most likely due to loss of microbial metabolites, signalling molecules, or regulators of host pathways. Modern life-style diseases such as Inflammatory Bowel Disease (IBD), Irritable Bowel Syndrome (IBS), type 2 diabetes, obesity and metabolic syndrome have been linked to dysbiosis...
July 11, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28695465/trichoderma-for-climate-resilient-agriculture
#15
REVIEW
Prem Lal Kashyap, Pallavi Rai, Alok Kumar Srivastava, Sudheer Kumar
Climate change is one of the biggest challenges of the twenty-first century for sustainable agricultural production. Several reports highlighted the need for better agricultural practices and use of eco-friendly methods for sustainable crop production under such situations. In this context, Trichoderma species could be a model fungus to sustain crop productivity. Currently, these are widely used as inoculants for biocontrol, biofertilization, and phytostimulation. They are reported to improve photosynthetic efficiency, enhance nutrient uptake and increase nitrogen use efficiency in crops...
August 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28690679/trichoderma-reesei-complete-genome-sequence-repeat-induced-point-mutation-and-partitioning-of-cazyme-gene-clusters
#16
Wan-Chen Li, Chien-Hao Huang, Chia-Ling Chen, Yu-Chien Chuang, Shu-Yun Tung, Ting-Fang Wang
BACKGROUND: Trichoderma reesei (Ascomycota, Pezizomycotina) QM6a is a model fungus for a broad spectrum of physiological phenomena, including plant cell wall degradation, industrial production of enzymes, light responses, conidiation, sexual development, polyketide biosynthesis, and plant-fungal interactions. The genomes of QM6a and its high enzyme-producing mutants have been sequenced by second-generation-sequencing methods and are publicly available from the Joint Genome Institute. While these genome sequences have offered useful information for genomic and transcriptomic studies, their limitations and especially their short read lengths make them poorly suited for some particular biological problems, including assembly, genome-wide determination of chromosome architecture, and genetic modification or engineering...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28690620/sugarcane-water-stress-tolerance-mechanisms-and-its-implications-on-developing-biotechnology-solutions
#17
REVIEW
Thais H S Ferreira, Max S Tsunada, Denis Bassi, Pedro Araújo, Lucia Mattiello, Giovanna V Guidelli, Germanna L Righetto, Vanessa R Gonçalves, Prakash Lakshmanan, Marcelo Menossi
Sugarcane is a unique crop with the ability to accumulate high levels of sugar and is a commercially viable source of biomass for bioelectricity and second-generation bioethanol. Water deficit is the single largest abiotic stress affecting sugarcane productivity and the development of water use efficient and drought tolerant cultivars is an imperative for all major sugarcane producing countries. This review summarizes the physiological and molecular studies on water deficit stress in sugarcane, with the aim to help formulate more effective research strategies for advancing our knowledge on genes and mechanisms underpinning plant response to water stress...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28690133/the-complete-genome-sequence-of-bacillus-velezensis-strain-gh1-13-reveals-agriculturally-beneficial-properties-and-a-unique-plasmid
#18
Sang Yoon Kim, Hajin Song, Mee Kyung Sang, Hang-Yeon Weon, Jaekyeong Song
The bacterial strain Bacillus velezensis GH1-13, isolated from rice paddy soil in Korea, has been shown to promote plant growth and have strong antagonistic activities against pathogens. Here, we report the complete genome sequence of GH1-13, revealing that it possesses a single 4,071,980-bp circular chromosome with 46.2% GC-content. The chromosome encodes 3,930 genes, and we have also identified a unique plasmid in the strain that encodes a further 104 genes (71,628bp and 31.7% GC-content). The genome was found to contain various enzyme-encoding operons, including indole-3-acetic acid (IAA) biosynthesis proteins, 2,3-butanediol dehydrogenase, various non-ribosomal peptide synthetases, and several polyketide synthases...
July 6, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28688929/plant-glyco-biotechnology
#19
REVIEW
Jennifer Schoberer, Richard Strasser
Glycosylation is an important protein modification in all eukaryotes. Whereas the early asparagine-linked glycosylation (N-glycosylation) and N-glycan processing steps in the endoplasmic reticulum are conserved between mammals and plants, the maturation of complex N-glycans in the Golgi apparatus differs considerably. Due to a restricted number of Golgi-resident N-glycan processing enzymes and the absence of nucleotide sugars such as CMP-N-acetylneuraminic acid, plants produce only a limited repertoire of different N-glycan structures...
July 5, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28686269/iron-homeostasis-in-plants-a-brief-overview
#20
James M Connorton, Janneke Balk, Jorge Rodríguez-Celma
Iron plays a crucial role in biochemistry and is an essential micronutrient for plants and humans alike. Although plentiful in the Earth's crust it is not usually found in a form readily accessible for plants to use. They must therefore sense and interact with their environment, and have evolved two different molecular strategies to take up iron in the root. Once inside, iron is complexed with chelators and distributed to sink tissues where it is used predominantly in the production of enzyme cofactors or components of electron transport chains...
July 19, 2017: Metallomics: Integrated Biometal Science
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