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https://www.readbyqxmd.com/read/27933261/efficient-regeneration-system-from-rye-leaf-base-segments
#1
Kamil Haliloglu, Murat Aydin
Rye is second only to wheat among grains most widely used in the making of bread and is also a very important gene resource for breeding and improvement of wheat and other cereal crops owing to tolerance to abiotic stress factors such as low temperatures, drought and poor soil conditions. However, application of biotechnologies has been limited in rye breeding since it is one of the most recalcitrant species in tissue culture. A simple and fast regeneration system from leaf-base segment explant of rye was developed in this study...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27930707/native-phytoremediation-potential-of-urtica-dioica-for-removal-of-pcbs-and-heavy-metals-can-be-improved-by-genetic-manipulations-using-constitutive-camv-35s-promoter
#2
Jitka Viktorova, Zuzana Jandova, Michaela Madlenakova, Petra Prouzova, Vilem Bartunek, Blanka Vrchotova, Petra Lovecka, Lucie Musilova, Tomas Macek
Although stinging nettle (Urtica dioica) has been shown to reduce HM (heavy metal) content in soil, its wider phytoremediation potential has been neglected. Urtica dioica was cultivated in soils contaminated with HMs or polychlorinated biphenyls (PCBs). After four months, up to 33% of the less chlorinated biphenyls and 8% of HMs (Zn, Pb, Cd) had been removed. Bacteria were isolated from the plant tissue, with the endophytic bacteria Bacillus shackletonii and Streptomyces badius shown to have the most significant effect...
2016: PloS One
https://www.readbyqxmd.com/read/27926813/perturbation-of-h3k27me3-associated-epigenetic-processes-increases-agrobacterium-mediated-transformation
#3
Hidekazu Iwakawa, Benjamin C Carter, Brett C Bishop, Joe Ogas, Stanton B Gelvin
Agrobacterium-mediated transformation is a core technology for basic plant science and agricultural biotechnology. Improving transformation frequency is a major goal for plant transgenesis. We previously showed that T-DNA insertions in some histone genes decreased transformation susceptibility, whereas overexpression of several Arabidopsis H2A and H4 isoforms increased transformation. Overexpression of several histone H2B and H3 isoforms had little effect on transformation frequency. However, overexpression of histone H3-11 (HTR11) enhanced transformation...
December 7, 2016: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/27925500/current-understandings-on-plant-nonhost-resistance
#4
Hyun-Ah Lee, Hye-Young Lee, Eunyoung Seo, Joohyun Lee, Saet-Byul Kim, SooHyun Oh, Eunbi Choi, Eunhye Choi, So Eui Lee, Doil Choi
Nonhost resistance, a resistance of plant species against all non-adapted pathogens, is considered the most durable and efficient immune system of plants but yet remains elusive. The underlying mechanism of nonhost resistance has been investigated at multiple levels of plant defense for several decades. In this review, we have comprehensively surveyed the latest literature on nonhost resistance in terms of pre-invasion, metabolic defense, pattern-triggered immunity, effector-triggered immunity, defense signaling, and possible application in crop protection...
December 7, 2016: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/27924530/aspergillus-species-and-their-associated-mycotoxins
#5
Giancarlo Perrone, Antonia Gallo
The genus Aspergillus is among the most abundant and widely distributed organism on earth, and at the moment comprises 339 known species. It is one of the most important economically fungal genus and the biotechnological use of Aspergillus species is related to production of soy sauce, of different hydrolytic enzymes (amylases, lipases) and organic acid (citric acid, gluconic acid), as well as biologically active metabolites such as lovastatin. Although they are not considered to be major cause of plant diseases, Aspergillus species are responsible for several disorders in various plants and plant products, especially as opportunistic storage moulds...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27916552/plenty-is-no-plague-streptomyces-symbiosis-with-crops
#6
REVIEW
Thomas Rey, Bernard Dumas
Streptomyces spp. constitute a major clade of the phylum Actinobacteria. These Gram-positive, filamentous prokaryotes are ubiquitous in soils and marine sediments, and are commonly found in the rhizosphere or inside plant roots. Plant-interacting Streptomyces have received limited attention, in contrast to Streptomyces spp. extensively investigated for decades in medicine given their rich potential for secondary metabolite biosynthesis. Recent genomic, metabolomic, and biotechnological advances have produced key insights into Streptomyces spp...
November 5, 2016: Trends in Plant Science
https://www.readbyqxmd.com/read/27915455/overexpression-of-atedt1-promotes-root-elongation-and-affects-medicinal-secondary-metabolite-biosynthesis-in-roots-of-transgenic-salvia-miltiorrhiza
#7
Yu Liu, Geng Sun, Zhaohui Zhong, Linyi Ji, Yong Zhang, Jianping Zhou, Xuelian Zheng, Kejun Deng
Medicinal secondary metabolites (salvianolic acids and tanshinones) are valuable natural bioactive compounds in Salvia miltiorrhiza and have widespread applications. Improvement of medicinal secondary metabolite accumulation through biotechnology is necessary and urgent to satisfy their increasing demand. Herein, it was demonstrated that the overexpression of the transcription factor Arabidopsis thaliana-enhanced drought tolerance 1 (AtEDT1) could affect medicinal secondary metabolite accumulation. In this study, we observed that the transgenic lines significantly conferred drought tolerance phenotype...
December 3, 2016: Protoplasma
https://www.readbyqxmd.com/read/27915147/highly-sensitive-optical-interferometric-technique-reveals-stress-dependent-instantaneous-nanometric-growth-fluctuations-of-chinese-chive-leaf-under-heavy-metal-stress
#8
Kokge Thilini Kanchana Muthumali DeSilva, Uma Maheswari Rajagopalan, Hirofumi Kadono
Plant growth apart from being a complex and highly dynamic is dependent on its immediate environment. Leaf expansion measurements using Statistical Interferometry Technique, a sensitive interferometric technique at nanometric accuracy and at sub-second levels revealed the presence of characteristic nanometric intrinsic fluctuations [Plant Biotechnology 31, 195 (2014)]. In this paper, we demonstrate that the nanometric intrinsic fluctuations are sensitive enough that they change under exposure of heavy metals, essential micronutrient zinc and non-essential element cadmium, at relatively low concentrations in the leaves of Chinese chive (Allium tuberosum)...
December 1, 2016: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/27910910/genome-wide-characterisation-and-expression-profile-of-the-grapevine-atl-ubiquitin-ligase-family-reveal-biotic-and-abiotic-stress-responsive-and-development-related-members
#9
Pietro Ariani, Alice Regaiolo, Arianna Lovato, Alejandro Giorgetti, Andrea Porceddu, Salvatore Camiolo, Darren Wong, Simone Castellarin, Elodie Vandelle, Annalisa Polverari
The Arabidopsis Tóxicos en Levadura (ATL) protein family is a class of E3 ubiquitin ligases with a characteristic RING-H2 Zn-finger structure that mediates diverse physiological processes and stress responses in plants. We carried out a genome-wide survey of grapevine (Vitis vinifera L.) ATL genes and retrieved 96 sequences containing the canonical ATL RING-H2 domain. We analysed their genomic organisation, gene structure and evolution, protein domains and phylogenetic relationships. Clustering revealed several clades, as already reported in Arabidopsis thaliana and rice (Oryza sativa), with an expanded subgroup of grapevine-specific genes...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27909993/impact-of-culture-conditions-on-the-chlorophyll-content-of-microalgae-for-biotechnological-applications
#10
REVIEW
Veronica da Silva Ferreira, Celso Sant'Anna
Chlorophyll is a commercially important natural green pigment responsible for the absorption of light energy and its conversion into chemical energy via photosynthesis in plants and algae. This bioactive compound is widely used in the food, cosmetic, and pharmaceutical industries. Chlorophyll has been consumed for health benefits as a nutraceutical agent with antioxidant, anti-inflammatory, antimutagenic, and antimicrobial properties. Microalgae are photosynthesizing microorganisms which can be extracted for several high-value bioproducts in the biotechnology industry...
January 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27908237/plant-factories-for-the-production-of-monoclonal-antibodies
#11
REVIEW
E V Sheshukova, T V Komarova, Y L Dorokhov
Like animal cells, plant cells bear mechanisms for protein synthesis and posttranslational modification (glycosylation and phosphorylation) that allow them to be seriously considered as factories for therapeutic proteins, including antibodies, with the development of biotechnology. The plant platform for monoclonal antibody production is an attractive approach due to its flexibility, speed, scalability, low cost of production, and lack of contamination risk from animal-derived pathogens. Contemporary production approaches for therapeutic proteins rely on transgenic plants that are obtained via the stable transformation of plant cells as well as the transient (temporary) expression of foreign proteins...
October 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27907207/optimized-jasmonic-acid-production-by-lasiodiplodia-theobromae-reveals-formation-of-valuable-plant-secondary-metabolites
#12
Felipe Eng, Sven Haroth, Kirstin Feussner, Dorothea Meldau, Dmitrij Rekhter, Till Ischebeck, Florian Brodhun, Ivo Feussner
Jasmonic acid is a plant hormone that can be produced by the fungus Lasiodiplodia theobromae via submerged fermentation. From a biotechnological perspective jasmonic acid is a valuable feedstock as its derivatives serve as important ingredients in different cosmetic products and in the future it may be used for pharmaceutical applications. The objective of this work was to improve the production of jasmonic acid by L. theobromae strain 2334. We observed that jasmonic acid formation is dependent on the culture volume...
2016: PloS One
https://www.readbyqxmd.com/read/27905924/current-knowledge-and-perspectives-of-paenibacillus-a-review
#13
REVIEW
Elliot Nicholas Grady, Jacqueline MacDonald, Linda Liu, Alex Richman, Ze-Chun Yuan
Isolated from a wide range of sources, the genus Paenibacillus comprises bacterial species relevant to humans, animals, plants, and the environment. Many Paenibacillus species can promote crop growth directly via biological nitrogen fixation, phosphate solubilization, production of the phytohormone indole-3-acetic acid (IAA), and release of siderophores that enable iron acquisition. They can also offer protection against insect herbivores and phytopathogens, including bacteria, fungi, nematodes, and viruses...
December 1, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27902938/functional-components-of-the-bacterial-czccba-efflux-system-reduce-cadmium-uptake-and-accumulation-in-transgenic-tobacco-plants
#14
Andrea Nesler, Giovanni DalCorso, Elisa Fasani, Anna Manara, Gian Pietro Di Sansebastiano, Emanuele Argese, Antonella Furini
Cadmium (Cd) is a toxic trace element released into the environment by industrial and agricultural practices, threatening the health of plants and contaminating the food/feed chain. Biotechnology can be used to develop plant varieties with a higher capacity for Cd accumulation (for use in phytoremediation programs) or a lower capacity for Cd accumulation (to reduce Cd levels in food and feed). Here we generated transgenic tobacco plants expressing components of the Pseudomonas putida CzcCBA efflux system. Plants were transformed with combinations of the CzcC, CzcB and CzcA genes, and the impact on Cd mobilization was analysed...
November 27, 2016: New Biotechnology
https://www.readbyqxmd.com/read/27902803/alphasim-software-for-breeding-program-simulation
#15
Anne-Michelle Faux, Gregor Gorjanc, R Chris Gaynor, Mara Battagin, Stefan M Edwards, David L Wilson, Sarah J Hearne, Serap Gonen, John M Hickey
This paper describes AlphaSim, a software package for simulating plant and animal breeding programs. AlphaSim enables the simulation of multiple aspects of breeding programs with a high degree of flexibility. AlphaSim simulates breeding programs in a series of steps: (i) simulate haplotype sequences and pedigree; (ii) drop haplotypes into the base generation of the pedigree and select single-nucleotide polymorphism (SNP) and quantitative trait nucleotide (QTN); (iii) assign QTN effects, calculate genetic values, and simulate phenotypes; (iv) drop haplotypes into the burn-in generations; and (v) perform selection and simulate new generations...
November 2016: Plant Genome
https://www.readbyqxmd.com/read/27900528/the-thermophilic-biomass-degrading-fungus-thielavia-terrestris-co3bag1-produces-a-hyperthermophilic-and-thermostable-%C3%AE-1-4-xylanase-with-exo-and-endo-activity
#16
Yolanda García-Huante, Maribel Cayetano-Cruz, Alejandro Santiago-Hernández, Claudia Cano-Ramírez, Rodolfo Marsch-Moreno, Jorge E Campos, Guillermo Aguilar-Osorio, Claudia G Benitez-Cardoza, Sergio Trejo-Estrada, María Eugenia Hidalgo-Lara
A hyperthermophilic and thermostable xylanase of 82 kDa (TtXynA) was purified from the culture supernatant of T. terrestris Co3Bag1, grown on carboxymethyl cellulose (CMC), and characterized biochemically. TtXynA showed optimal xylanolytic activity at pH 5.5 and at 85 °C, and retained more than 90% of its activity at a broad pH range (4.5-10). The enzyme is highly thermostable with a half-life of 23.1 days at 65 °C, and active in the presence of several metal ions. Circular dichroism spectra strongly suggest the enzyme gains secondary structures when temperature increases...
November 29, 2016: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/27898811/de-novo-transcriptome-assembly-and-dynamic-spatial-gene-expression-analysis-in-red-clover
#17
Manohar Chakrabarti, Randy D Dinkins, Arthur G Hunt
Red clover ( L.) is a cool-season forage legume grown throughout the northeastern United States and is the most widely planted forage legume after alfalfa ( L.). Red clover provides high-value feed to the livestock because of high protein content and easy digestibility. To date, genomic resources for red clover are scarce. In the current study, a de novo transcriptome assembly of red clover was constructed representing different tissue types. The draft assembly consists of 37,565 contigs with N50 and average contig length of 1707 and 1262 bp, respectively...
July 2016: Plant Genome
https://www.readbyqxmd.com/read/27898188/modern-approaches-to-discovering-new-hydroxynitrile-lyases-for-biocatalysis
#18
REVIEW
Santosh Kumar Padhi
Hydroxynitrile lyases (HNLs) have grown in importance from laboratory to industry due to their potential to catalyze stereoselective C-C bond-formation reactions in the synthesis of several chiral intermediates, such as enantiopure α-cyanohydrins, β-nitro alcohols, and their derivatives with multiple functional groups. With these wide applications, the demand for finding new HNLs has increased, and this has led to exploration not only of new HNLs but also of new ways to discover them. An exclusive review article on HNLs by Asano et al...
November 29, 2016: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/27892924/the-pattern-recognition-receptor-core-of-solanaceae-detects-bacterial-cold-shock-protein
#19
Lei Wang, Markus Albert, Elias Einig, Ursula Fürst, Damaris Krust, Georg Felix
Plants and animals recognize microbial invaders by detecting microbe-associated molecular patterns (MAMPs) by cell surface receptors. Many plant species of the Solanaceae family detect the highly conserved nucleic acid binding motif RNP-1 of bacterial cold-shock proteins (CSPs), represented by the peptide csp22, as a MAMP. Here, we exploited the natural variation in csp22 perception observed between cultivated tomato (Solanum lycopersicum) and Solanum pennellii to map and identify the leucine-rich repeat (LRR) receptor kinase CORE (cold shock protein receptor) of tomato as the specific, high-affinity receptor site for csp22...
November 28, 2016: Nature Plants
https://www.readbyqxmd.com/read/27892643/the-rice-osnac6-transcription-factor-orchestrates-multiple-molecular-mechanisms-involving-root-structural-adaptions-and-nicotianamine-biosynthesis-for-drought-tolerance
#20
Dong-Keun Lee, Pil Joong Chung, Jin Seo Jeong, Geupil Jang, Seung Woon Bang, Harin Jung, Youn Shic Kim, Sun-Hwa Ha, Yang Do Choi, Ju-Kon Kim
Drought has a serious impact on agriculture worldwide. A plant's ability to adapt to rhizosphere drought stress requires reprograming of root growth and development. Although physiological studies have documented the root adaption for tolerance to the drought stress, underlying molecular mechanisms is still incomplete, which is essential for crop engineering. Here, we identified OsNAC6-mediated root structural adaptations, including increased root number and root diameter, which enhanced drought tolerance. Multiyear drought field tests demonstrated that the grain yield of OsNAC6 root-specific overexpressing transgenic rice lines was less affected by drought stress than were non-transgenic controls...
November 28, 2016: Plant Biotechnology Journal
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