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https://www.readbyqxmd.com/read/28429057/characterisation-of-three-fungal-glucuronoyl-esterases-on-glucuronic-acid-ester-model-compounds
#1
Silvia Hüttner, Sylvia Klaubauf, Ronald P de Vries, Lisbeth Olsson
The glucuronoyl esterases (GEs) that have been identified so far belong to family 15 of the carbohydrate esterases in the CAZy classification system and are presumed to target ester bonds between lignin alcohols and (4-O-methyl-)D-glucuronic acid residues of xylan. Few GEs have been cloned, expressed and characterised to date. Characterisation has been done on a variety of synthetic substrates; however, the number of commercially available substrates is very limited. We identified novel putative GEs from a wide taxonomic range of fungi and expressed the enzymes originating from Acremonium alcalophilum and Wolfiporia cocos as well as the previously described PcGE1 from Phanerochaete chrysosporium...
April 20, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28428782/a-recombinant-potato-virus-y-infectious-clone-tagged-with-the-rosea1-visual-marker-pvy-ros1-facilitates-the-analysis-of-viral-infectivity-and-allows-the-production-of-large-amounts-of-anthocyanins-in-plants
#2
Teresa Cordero, Mohamed A Mohamed, Juan-José López-Moya, José-Antonio Daròs
Potato virus Y (PVY) is a major threat to the cultivation of potato and other solanaceous plants. By inserting a cDNA coding for the Antirrhinum majus Rosea1 transcription factor into a PVY infectious clone, we created a biotechnological tool (PVY-Ros1) that allows infection by this relevant plant virus to be tracked by the naked eye with no need for complex instrumentation. Rosea1 is an MYB-type transcription factor whose expression activates the biosynthesis of anthocyanin pigments in a dose-specific and cell-autonomous manner...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28428046/cryoprotectants-a-review-of-the-actions-and-applications-of-cryoprotective-solutes-that-modulate-cell-recovery-from-ultra-low-temperatures
#3
REVIEW
Gloria D Elliott, Shangping Wang, Barry J Fuller
Cryopreservation has become a central technology in many areas of clinical medicine, biotechnology, and species conservation within both plant and animal biology. Cryoprotective agents (CPAs) invariably play key roles in allowing cells to be processed for storage at deep cryogenic Temperatures and to be recovered with high levels of appropriate functionality. As such, these CPA solutes possess a wide range of metabolic and biophysical effects that are both necessary for their modes of action, and potentially complicating for cell biological function...
April 18, 2017: Cryobiology
https://www.readbyqxmd.com/read/28427274/first-principle-based-models-in-plant-suspension-cell-cultures-a-review
#4
Adriana Villegas, Juan Pablo Arias, Daira Aragón, Silvia Ochoa, Mario Arias
In this work, the development and application of published models for describing the behavior of plant cell cultures is reviewed. The structure of each type of model is analyzed and the new tendencies for the modeling of biotechnological processes that can be applied in plant cell cultures are presented. This review is a tool for clarifying the main features that characterize each type of model in the field of plant cell cultures and can be used as a support on the selection of the more suitable model type, taking into account the purpose of specific research...
April 21, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/28426952/plant-viruses-and-bacteriophages-for-delivery-in-medicine-and-biotechnology
#5
REVIEW
Anna E Czapar, Nicole F Steinmetz
There are a wide variety of synthetic and naturally occurring nanomaterials under development for nanoscale cargo-delivery applications. Viruses play a special role in these developments, because they can be regarded as naturally occurring nanomaterials evolved to package and deliver cargos. While any nanomaterial has its advantage and disadvantages, viral nanoparticles (VNPs), in particular the ones derived from plant viruses and bacteriophages, are attractive options for cargo-delivery as they are biocompatible, biodegradable, and non-infectious to mammals...
April 17, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28424569/current-knowledge-of-schisandra-chinensis-turcz-baill-chinese-magnolia-vine-as-a-medicinal-plant-species-a-review-on-the-bioactive-components-pharmacological-properties-analytical-and-biotechnological-studies
#6
REVIEW
Agnieszka Szopa, Radosław Ekiert, Halina Ekiert
Schisandra chinensis Turcz. (Baill.) is a plant species whose fruits have been well known in Far Eastern medicine for a long time. However, schisandra seems to be a plant still underestimated in contemporary therapy still in the countries of East Asia. The article presents latest available information on the chemical composition of this plant species. Special attention is given to dibenzo cyclooctadiene lignans. In addition, recent studies of the biological activity of dibenzocyclooctadiene lignans and schisandra fruit extracts are recapitulated...
2017: Phytochemistry Reviews: Proceedings of the Phytochemical Society of Europe
https://www.readbyqxmd.com/read/28423946/tween-80-surfactant-enhanced-bioremediation-toward-a-solution-to-the-soil-contamination-by-hydrophobic-organic-compounds
#7
Min Cheng, Guangming Zeng, Danlian Huang, Chunping Yang, Cui Lai, Chen Zhang, Yang Liu
The occurrence of hydrophobic organic compounds (HOCs) in the soil has become a highly significant environmental issue. This problem has been exacerbated by the strong sorption of HOCs to the soils, which makes them unavailable for most remediation processes. More and more works show that surfactant-enhanced biological technologies offer a great potential to clear up HOCs-contaminated soils. This article is a critical review of HOCs removal from soils using Tween 80 (one of the mostly used nonionic surfactants) aided biological remediation technologies...
April 20, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/28419345/dehydration-responsive-element-binding-transcription-factors-and-their-applications-for-the-engineering-of-stress-tolerance
#8
Pradeep K Agarwal, Kapil Gupta, Sergiy Lopato, Parinita Agarwal
Dehydration responsive element binding (DREB) factors or CRT element binding factors (CBFs) are members of the AP2/ERF family, which comprises a large number of stress-responsive regulatory genes. This review traverses almost two decades of research, from the discovery of DREB/CBF factors to their optimization for application in plant biotechnology. In this review, we describe (i) the discovery, classification, structure, and evolution of DREB genes and proteins; (ii) induction of DREB genes by abiotic stresses and involvement of their products in stress responses; (iii) protein structure and DNA binding selectivity of different groups of DREB proteins; (iv) post-transcriptional and post-translational mechanisms of DREB transcription factor (TF) regulation; and (v) physical and/or functional interaction of DREB TFs with other proteins during plant stress responses...
April 17, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28414527/advantages-and-limitations-of-in-vitro-and-in-vivo-methods-of-iron-and-zinc-bioavailability-evaluation-in-the-assessment-of-biofortification-program-effectiveness
#9
Desirrê Morais Dias, Neuza Maria Brunoro Costa, Marilia Regini Nutti, Elad Tako, Hércia Stampini Duarte Martino
Biofortification aims to improve the micronutrient concentration of staple food crops through the best practices of breeding and modern biotechnology. However, increased zinc and iron concentrations in food crops may not always translate into proportional increases in absorbed zinc (Zn) and iron (Fe). Therefore, assessing iron and zinc bioavailability in biofortified crops is imperative to evaluate the efficacy of breeding programs. This review aimed to investigate the advantages and limitations of in vitro and in vivo methods of iron and zinc bioavailability evaluation in the assessment of biofortification program effectiveness...
April 17, 2017: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/28411481/a-re-evaluation-of-the-final-step-of-vanillin-biosynthesis-in-the-orchid-vanilla-planifolia
#10
Hailian Yang, Jaime Barros-Rios, Galina Kourteva, Xiaolan Rao, Fang Chen, Hui Shen, Chenggang Liu, Andrzej Podstolski, Faith Belanger, Daphna Havkin-Frenkel, Richard A Dixon
A recent publication describes an enzyme from the vanilla orchid Vanilla planifolia with the ability to convert ferulic acid directly to vanillin. The authors propose that this represents the final step in the biosynthesis of vanillin, which is then converted to its storage form, glucovanillin, by glycosylation. The existence of such a "vanillin synthase" could enable biotechnological production of vanillin from ferulic acid using a "natural" vanilla enzyme. The proposed vanillin synthase exhibits high identity to cysteine proteases, and is identical at the protein sequence level to a protein identified in 2003 as being associated with the conversion of 4-coumaric acid to 4-hydroxybenzaldehyde...
April 12, 2017: Phytochemistry
https://www.readbyqxmd.com/read/28409382/yeast-response-and-tolerance-to-benzoic-acid-involves-the-gcn4-and-stp1-regulated-multidrug-multixenobiotic-resistance-transporter-tpo1
#11
Cláudia P Godinho, Nuno P Mira, Tânia R Cabrito, Miguel C Teixeira, Kaur Alasoo, Joana F Guerreiro, Isabel Sá-Correia
The action of benzoic acid in the food and beverage industries is compromised by the ability of spoilage yeasts to cope with this food preservative. Benzoic acid occurs naturally in many plants and is an intermediate compound in the biosynthesis of many secondary metabolites. The understanding of the mechanisms underlying the response and resistance to benzoic acid stress in the eukaryotic model yeast is thus crucial to design more suitable strategies to deal with this toxic lipophilic weak acid. In this study, the Saccharomyces cerevisiae multidrug transporter Tpo1 was demonstrated to confer resistance to benzoic acid...
April 13, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28407010/comparative-molecular-cytogenetic-characterization-of-seven-deschampsia-poaceae-species
#12
COMPARATIVE STUDY
Alexandra V Amosova, Nadezhda L Bolsheva, Svyatoslav A Zoshchuk, Maryana O Twardovska, Olga Yu Yurkevich, Igor O Andreev, Tatiana E Samatadze, Ekaterina D Badaeva, Viktor A Kunakh, Olga V Muravenko
The genus Deschampsia P. Beauv (Poaceae) involves a group of widespread polymorphic species. Some of them are highly tolerant to stressful and variable environmental conditions, and D. antarctica is one of the only two vascular plants growing in Antarctic. This species is a source of useful for selection traits and a valuable model for studying an environmental stress tolerance in plants. Genome diversity and comparative chromosomal phylogeny within the genus have not been studied yet as karyotypes of most Deschampsia species are poorly investigated...
2017: PloS One
https://www.readbyqxmd.com/read/28403831/the-role-of-tre6p-and-snrk1-in-maize-early-kernel-development-and-events-leading-to-stress-induced-kernel-abortion
#13
Samuel W Bledsoe, Clémence Henry, Cara A Griffiths, Matthew J Paul, Regina Feil, John E Lunn, Mark Stitt, L Mark Lagrimini
BACKGROUND: Drought stress during flowering is a major contributor to yield loss in maize. Genetic and biotechnological improvement in yield sustainability requires an understanding of the mechanisms underpinning yield loss. Sucrose starvation has been proposed as the cause for kernel abortion; however, potential targets for genetic improvement have not been identified. Field and greenhouse drought studies with maize are expensive and it can be difficult to reproduce results; therefore, an in vitro kernel culture method is presented as a proxy for drought stress occurring at the time of flowering in maize (3 days after pollination)...
April 12, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28400175/recombinant-plant-derived-human-ige-glycoproteomics
#14
Laura Montero-Morales, Daniel Maresch, Alexandra Castilho, Aysegül Turupcu, Kristina Ilieva, Silvia Crescioli, Sophia Karagiannis, Christian Lupinek, Chris Oostenbrink, Friedrich Altmann, Herta Steinkellner
The increasing biotechnological interest in human IgE antibodies demands advanced systems which allow their proper expression. However, this is still a challenge due to the complexity of the molecule, particularly regarding the diverse N-glycosylation pattern. Here, we present the expression of recombinant IgE in wild type and glycan-engineered Nicotiana benthamiana plants and in-depth N-glycosylation analyses. Mass spectrometric profiling revealed that plant IgE has a site occupancy rate that ranges from non-occupied at glycosite 6 (GS6) to 100% occupancy at GS1 and 2...
April 8, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28400173/strigolactones-in-plant-interactions-with-beneficial-and-detrimental-organisms-the-yin-and-yang
#15
REVIEW
Juan A López-Ráez, Ken Shirasu, Eloise Foo
Strigolactones (SLs) are plant hormones that have important roles as modulators of plant development. They were originally described as ex planta signaling molecules in the rhizosphere that induce the germination of parasitic plants, a role that was later linked to encouraging the beneficial symbiosis with arbuscular mycorrhizal (AM) fungi. Recently, the focus has shifted to examining the role of SLs in plant-microbe interactions, and has revealed roles for SLs in the association of legumes with nitrogen-fixing rhizobacteria and in interactions with disease-causing pathogens...
April 8, 2017: Trends in Plant Science
https://www.readbyqxmd.com/read/28399663/upstream-biomanufacturing-of-pharmaceutical-colchicine
#16
Ganapathy Sivakumar
The plant-based colchicine potentially affects major diseases such as cardiovascular events, cancers and gout. Gloriosa superba seeds are a conventional pharmaceutical source of colchicine. The demand for pharmaceutical-grade colchicine has increased due to the shortage of feasible upstream manufacturing, encompassing all stages in the processes of biosynthesis and biomanufacturing before the raw material is ready for purification. Consequently, developing sustainable upstream industrial colchicine biofactories is imperative, especially in curtailing drug costs...
April 12, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/28395514/exiguobacterium-an-overview-of-a-versatile-genus-with-potential-in-industry-and-agriculture
#17
Ramesh C Kasana, C B Pandey
The bacterial genus Exiguobacterium accommodates many versatile species isolated from diverse environments. Exiguobacterium was described as a genus approximately three decades ago, and now, 17 species, growing over a broad range of temperatures and pH, have been recognized. Various isolates from different niches have been explored for biotechnological and industrial purposes, including enzyme production, bioremediation and degradation of toxic substances released into the environment. Some isolates possess plant growth promoting capabilities, and they are currently being explored for increasing agricultural production...
April 11, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/28393691/biotechnological-approaches-for-production-of-anti-cancerous-compounds-resveratrol-podophyllotoxin-and-zerumbone
#18
Krishnadas Nandagopal, Mihir Halder, Biswabhusan Dash, Sanghamitra Nayak, Sumita Jha
Secondary metabolites from numerous plant sources have been developed as anti-cancer reagents and compounds such as resveratrol, podophyllotoxin and zerumbone are of particular importance in this regard. Since their de novo chemical synthesis is both arduous and commercially expensive, there has been an impetus to develop viable, biotechnological methods of production. Accordingly, this review focuses on recent developments in the field, highlighting the use of micropropagation, cell suspension cultures, callus cultures, hairy root cultures, recombinant microbes and genetically modified higher plants...
April 4, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28391618/structural-features-of-aspergillus-niger-%C3%AE-galactosidase-define-its-activity-against-glycoside-linkages
#19
Agustín Rico-Díaz, Mercedes Ramírez-Escudero, Ángel Vizoso-Vázquez, M Esperanza Cerdán, Manuel Becerra, Julia Sanz-Aparicio
β-galactosidases are biotechnologically interesting enzymes that catalyze the hydrolysis or transgalactosylation of β-galactosides. Among them, the Aspergillus niger β-galactosidase (AnβGal) belongs to the glycoside hydrolase family 35 (GH35) and is widely used in the industry due to its high hydrolytic activity degrading lactose. We present here its three-dimensional structure in complex with different oligosaccharides, to illustrate the structural determinants of the broad specificity of the enzyme against different glycoside linkages...
April 8, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28391528/plant-glutathione-transferase-mediated-stress-tolerance-functions-and-biotechnological-applications
#20
REVIEW
Irini Nianiou-Obeidat, Panagiotis Madesis, Christos Kissoudis, Georgia Voulgari, Evangelia Chronopoulou, Athanasios Tsaftaris, Nikolaos E Labrou
Plant glutathione transferases (EC 2.5.1.18, GSTs) are an ancient, multimember and diverse enzyme class. Plant GSTs have diverse roles in plant development, endogenous metabolism, stress tolerance, and xenobiotic detoxification. Their study embodies both fundamental aspects and agricultural interest, because of their ability to confer tolerance against biotic and abiotic stresses and to detoxify herbicides. Here we review the biotechnological applications of GSTs towards developing plants that are resistant to biotic and abiotic stresses...
April 8, 2017: Plant Cell Reports
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