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https://www.readbyqxmd.com/read/28334155/genetic-diversity-of-symbiotic-paraburkholderia-species-isolated-from-nodules-of-mimosa-pudica-l-and-phaseolus-vulgaris-l-grown-in-soils-of-the-brazilian-atlantic-forest-mata-atl%C3%A3-ntica
#1
Rebeca Fuzinatto Dall Agnol, Caroline Bournaud, Sérgio Miana de Faria, Gilles Béna, Lionel Moulin, Mariangela Hungria
Some species of the genus Paraburkholderia that are able to nodulate and fix nitrogen in symbiosis with legumes are called β-rhizobia and represent a group of ecological and biotechnological importance. We used Mimosa pudica and Phaseolus vulgaris to trap 427 rhizobial isolates from rhizospheric soil of Mimoseae trees in the Brazilian Atlantic Forest. Eighty-four representative strains were selected according to the 16S rRNA haplotypes and taxonomically characterized using a concatenated 16S rRNA-recA phylogeny...
February 23, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28332603/the-arabidopsis-mir396-mediates-pathogen-associated-molecular-pattern-triggered-immune-responses-against-fungal-pathogens
#2
Mauricio Soto-Suárez, Patricia Baldrich, Detlef Weigel, Ignacio Rubio-Somoza, Blanca San Segundo
MicroRNAs (miRNAs) play a pivotal role in regulating gene expression during plant development. Although a substantial fraction of plant miRNAs has proven responsive to pathogen infection, their role in disease resistance remains largely unknown, especially during fungal infections. In this study, we screened Arabidopsis thaliana lines in which miRNA activity has been reduced using artificial miRNA target mimics (MIM lines) for their response to fungal pathogens. Reduced activity of miR396 (MIM396 plants) was found to confer broad resistance to necrotrophic and hemibiotrophic fungal pathogens...
March 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28330304/elicitation-of-gymnemic-acid-production-in-cell-suspension-cultures-of-gymnema-sylvestre-r-br-through-endophytic-fungi
#3
Vasudeva Reddy Netala, Venkata Subbaiah Kotakadi, Susmila Aparna Gaddam, Sukhendu Bikash Ghosh, Vijaya Tartte
The enhancement of plant secondary metabolite production in cell suspension cultures through biotic or abiotic elicitation has become a potential biotechnological approach for commercialization or large-scale production of bioactive compounds. Gymnema sylvestre R.Br. is an important medicinal plant, rich in a group of oleanane triterpenoid saponins called gymnemic acid, well known for its anti-diabetic activity. Two endophytic fungal strains were isolated from the leaves of G. sylvestre and identified as Polyancora globosa and Xylaria sp...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330117/microbial-pectinases-an-ecofriendly-tool-of-nature-for-industries
#4
REVIEW
G Garg, A Singh, A Kaur, R Singh, J Kaur, R Mahajan
Pectinases are the growing enzymes of biotechnological sector, showing gradual increase in their market. They hold a leading position among the commercially produced industrial enzymes. These enzymes are ecofriendly tool of nature that are being used extensively in various industries like wine industry; food industry; paper industry for bleaching of pulp and waste paper recycling; in the processing of fruit-vegetables, tea-coffee, animal feed; extraction of vegetable oil and scouring of plant fibres. Moreover, enzymatic catalysis is preferred over other chemical methods, since it is more specific, less aggressive and saves energy...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330085/bioprospecting-and-biotechnological-applications-of-fungal-laccase
#5
REVIEW
Pooja Upadhyay, Rahul Shrivastava, Pavan Kumar Agrawal
Laccase belongs to a small group of enzymes called the blue multicopper oxidases, having the potential ability of oxidation. It belongs to enzymes, which have innate properties of reactive radical production, but its utilization in many fields has been ignored because of its unavailability in the commercial field. There are diverse sources of laccase producing organisms like bacteria, fungi and plants. In fungi, laccase is present in Ascomycetes, Deuteromycetes, Basidiomycetes and is particularly abundant in many white-rot fungi that degrade lignin...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330074/catalytic-properties-functional-attributes-and-industrial-applications-of-%C3%AE-glucosidases
#6
Gopal Singh, A K Verma, Vinod Kumar
β-Glucosidases are diverse group of enzymes with great functional importance to biological systems. These are grouped in multiple glycoside hydrolase families based on their catalytic and sequence characteristics. Most studies carried out on β-glucosidases are focused on their industrial applications rather than their endogenous function in the target organisms. β-Glucosidases performed many functions in bacteria as they are components of large complexes called cellulosomes and are responsible for the hydrolysis of short chain oligosaccharides and cellobiose...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330073/cladosporium-cladosporioides-from-the-perspectives-of-medical-and-biotechnological-approaches
#7
REVIEW
Manaf AlMatar, Essam A Makky
Fungi are important natural product sources that have enormous potential for the production of novel compounds for use in pharmacology, agricultural applications and industry. Compared with other natural sources such as plants, fungi are highly diverse but understudied. However, research on Cladosporium cladosporioides revealed the existence of bioactive products such as p-methylbenzoic acid, ergosterol peroxide (EP) and calphostin C as well as enzymes including pectin methylesterase (PME), polygalacturonase (PG) and chlorpyrifos hydrolase...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28326128/citric-acid-emerging-applications-of-key-biotechnology-industrial-product
#8
REVIEW
Rosaria Ciriminna, Francesco Meneguzzo, Riccardo Delisi, Mario Pagliaro
Owing to new biotechnological production units mostly located in China, global supply of citric acid in the course of the last two decades rose from less than 0.5 to more than 2 million tonnes becoming the single largest chemical obtained via biomass fermentation and the most widely employed organic acid. Critically reviewing selected research achievements and production trends, we identify the reasons for which this polycarboxylic acid will become a key chemical in the emerging bioeconomy.Graphical abstractPalermo's Fabbrica Chimica Italiana Goldenberg today...
2017: Chemistry Central Journal
https://www.readbyqxmd.com/read/28325847/specialized-diacylglycerol-acyltransferase-contributes-to-extreme-fatty-acid-content-of-cuphea-seed-oil
#9
Umidjon Iskandarov, Jillian E Silva, Hae Jin Kim, Mariette Andersson, Rebecca E Cahoon, Keithanne Mockaitis, Edgar B Cahoon
Seed oils of many Cuphea sp. contain >90% of medium-chain fatty acids, such as decanoic acid (10:0). These seed oils, which are among the most compositionally variant in the plant kingdom, arise from specialized fatty acid biosynthetic enzymes and specialized acyltransferases. These include lysophosphatidic acid acyltransferases (LPAT) and diacylglycerol acyltransferases (DGAT) that are required for successive acylation of medium-chain fatty acids in the sn-2 and sn-3 positions of seed triacylglycerols (TAGs)...
March 21, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28325321/biosynthesis-of-agar-in-red-seaweeds-a-review
#10
REVIEW
Wei-Kang Lee, Yi-Yi Lim, Adam Thean-Chor Leow, Parameswari Namasivayam, Janna Ong Abdullah, Chai-Ling Ho
Agar is a jelly-like biopolymer synthesized by many red seaweeds as their major cell wall component. Due to its excellent rheological properties, it has been exploited commercially for applications in food, cosmetic, pharmaceutical, biomedical and biotechnology industries. Despite its multiple uses, the biosynthesis of this phycocolloid is not fully understood. The current knowledge on agar biosynthesis is inferred from plant biochemistry and putative pathways for ulvan and alginate biosynthesis in green and brown seaweeds, respectively...
May 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28317006/proteomic-data-on-enzyme-secretion-and-activity-in-the-bacterium-chitinophaga-pinensis
#11
Johan Larsbrink, Tina R Tuveng, Phillip B Pope, Vincent Bulone, Vincent G H Eijsink, Harry Brumer, Lauren S McKee
The secretion of carbohydrate-degrading enzymes by a bacterium sourced from a softwood forest environment has been investigated by mass spectrometry. The findings are discussed in full in the research article "Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis" in Journal of Proteomics by Larsbrink et al. ([1], doi: 10.1016/j.jprot.2017.01.003). The bacterium was grown on three carbon sources (glucose, glucomannan, and galactomannan) which are likely to be nutrient sources or carbohydrate degradation products found in its natural habitat...
April 2017: Data in Brief
https://www.readbyqxmd.com/read/28316608/expression-of-16-nitrogenase-proteins-within-the-plant-mitochondrial-matrix
#12
Robert S Allen, Kimberley Tilbrook, Andrew C Warden, Peter C Campbell, Vivien Rolland, Surinder P Singh, Craig C Wood
The industrial production and use of nitrogenous fertilizer involves significant environmental and economic costs. Strategies to reduce fertilizer dependency are required to address the world's increasing demand for sustainable food, fibers, and biofuels. Biological nitrogen fixation, a process unique to diazatrophic bacteria, is catalyzed by the nitrogenase complex, and reconstituting this function in plant cells is an ambitious biotechnological strategy to reduce fertilizer use. Here we establish that the full array of biosynthetic and catalytic nitrogenase (Nif) proteins from the diazotroph Klebsiella pneumoniae can be individually expressed as mitochondrial targeting peptide (MTP)-Nif fusions in Nicotiana benthamiana...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28314933/major-genes-determining-yield-related-traits-in-wheat-and-barley
#13
REVIEW
Anna Nadolska-Orczyk, Izabela K Rajchel, Wacław Orczyk, Sebastian Gasparis
Current development of advanced biotechnology tools allows us to characterize the role of key genes in plant productivity. The implementation of this knowledge in breeding strategies might accelerate the progress in obtaining high-yielding cultivars. The achievements of the Green Revolution were based on a specific plant ideotype, determined by a single gene involved in gibberellin signaling or metabolism. Compared with the 1950s, an enormous increase in our knowledge about the biological basis of plant productivity has opened new avenues for novel breeding strategies...
March 17, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/28314149/cytokinin-response-in-pepper-plants-capsicum-annuum-l-exposed-to-silver-nanoparticles
#14
Tomislav Vinković, Ondrej Novák, Miroslav Strnad, Walter Goessler, Darija Domazet Jurašin, Nada Parađiković, Ivana Vinković Vrček
The increasing development of different nanomaterials, such as silver nanoparticles (AgNPs), and their practical use in agriculture and biotechnology has created a strong need for elucidations of biological effects and risk assessments of AgNPs in plants. This study was aimed to investigate AgNPs effects on metal uptake and their biodistribution in pepper plants as well as on morphological parameters and hormonal responses of the isoprenoid cytokinin (CK) family. In addition, the comparison of effects silver form, nanoparticles vs...
March 14, 2017: Environmental Research
https://www.readbyqxmd.com/read/28303457/lipid-metabolism-and-potentials-of-biofuel-and-high-added-value-oil-production-in-red-algae
#15
REVIEW
Naoki Sato, Takashi Moriyama, Natsumi Mori, Masakazu Toyoshima
Biomass production is currently explored in microalgae, macroalgae and land plants. Microalgal biofuel development has been performed mostly in green algae. In the Japanese tradition, macrophytic red algae such as Pyropia yezoensis and Gelidium crinale have been utilized as food and industrial materials. Researches on the utilization of unicellular red microalgae such as Cyanidioschyzon merolae and Porphyridium purpureum started only quite recently. Red algae have relatively large plastid genomes harboring more than 200 protein-coding genes that support the biosynthetic capacity of the plastid...
April 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28299544/biotechnology-of-the-medicinal-plant-rhazya-stricta-a-little-investigated-member-of-the-apocynaceae-family
#16
REVIEW
Amir Akhgari, Kirsi-Marja Oksman-Caldentey, Heiko Rischer
Rhazya stricta Decne. (Apocynaceae) is an important medicinal plant that is widely distributed in the Middle East and Indian sub-continent. It produces a large number of terpenoid indole alkaloids (TIAs) some of which possess important pharmacological properties. However, the yields of these compounds are very low. Establishment of a reliable, reproducible and efficient transformation method and induction of hairy roots system is a vital prerequisite for application of biotechnology in order to improve secondary metabolite yields...
March 15, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28299323/addressing-challenges-to-enhance-the-bioactives-of-withania-somnifera-through-organ-tissue-and-cell-culture-based-approaches
#17
REVIEW
Pritika Singh, Rupam Guleri, Amrita Angurala, Kuldeep Kaur, Kulwinder Kaur, Sunil C Kaul, Renu Wadhwa, Pratap Kumar Pati
Withania somnifera is a highly valued medicinal plant in traditional home medicine and is known for a wide range of bioactivities. Its commercial cultivation is adversely affected by poor seed viability and germination. Infestation by various pests and pathogens, survival under unfavourable environmental conditions, narrow genetic base, and meager information regarding biosynthesis of secondary metabolites are some of the other existing challenges in the crop. Biotechnological interventions through organ, tissue, and cell culture provide promising options for addressing some of these issues...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28294074/probiotics-as-a-tool-to-biosynthesize-metallic-nanoparticles-research-reports-and-patents-survey
#18
Nida Ahktar, Kamla Pathak
BACKGROUND: Probiotics are the live microbes that exert beneficial effects on the health of the host cell and thus creating novel nanoformulations via probiotic bacteria have gained tremendous momentum recently. The probiotic bacteria are being employed in synthesizing or more specifically biosynthesizing several nanoparticles like metallic as well as non-metallic nanoparticles. Biosynthesis of metallic nanoparticles is currently focused of research nowadays due to several applications thereof...
March 13, 2017: Recent Patents on Drug Delivery & Formulation
https://www.readbyqxmd.com/read/28292240/bioactive-metabolites-from-pathogenic-and-endophytic-fungi-of-forest-trees
#19
Marco Masi, Lucia Maddau, Benedetto Teodoro Linaldeddu, Bruno Scanu, Antonio Evidente, Alessio Cimmino
Fungi play an important role in terrestrial ecosystems interacting positively or negatively with plants. These interactions are complex and the outcomes are different depending on the fungal lifestyles, saprotrophic, mutualistic or pathogenic. Furthermore, fungi are well known for producing secondary metabolites, originating from different biosynthetic pathways, which possess biological properties of considerable biotechnological interest. Among the terrestrial ecosystems, temperate forests represent an enormous reservoir of fungal diversity...
March 14, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28288816/a-comparative-approach-to-recombinantly-produce-the-plant-enzyme-horseradish-peroxidase-in-escherichia-coli
#20
Thomas Gundinger, Oliver Spadiut
Horseradish peroxidase (HRP) is used in various biotechnological and medical applications. Since its isolation from plant provides several disadvantages, the bacterium Escherichia coli was tested as recombinant expression host in former studies. However, neither production from refolded inclusion bodies nor active enzyme expression in the periplasm exceeded final titres of 10mg per litre cultivation broth. Thus, the traditional way of production of HRP from plant still prevails. In this study, we revisited the recombinant production of HRP in E...
March 11, 2017: Journal of Biotechnology
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