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https://www.readbyqxmd.com/read/27919692/improved-production-of-2-fucosyllactose-in-engineered-escherichia-coli-by-expressing-putative-%C3%AE-1-2-fucosyltransferase-wcfb-from-bacteroides-fragilis
#1
Young-Wook Chin, Ji-Yeong Kim, Jae-Han Kim, Sang-Min Jung, Jin-Ho Seo
2'-Fucosyllactose (2-FL) is one of most abundant oligosaccharides in human milk, which is involved in many biological functions for infant health. Since 2-FL has a great potential in application to functional food materials and pharmaceuticals, several microbial systems for mass production of 2-FL have been developed in recent years. Microbial production of 2-FL was suggested to be influenced by a number of factors including fucosylation activity of α-1,2-fucosyltransferase. In the present study, the wcfB gene coding for α-1,2-fucosyltransferase from Bacteroides fragilis was screened from eleven candidates of putative α-1,2-fucosyltransferase...
December 2, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/27919152/-monitoring-plan-of-environmental-contaminants-in-sheep-milk-from-areas-next-to-an-italian-contaminated-site-named-%C3%A2-aree-industriali-di-porto-torres-e-discarica-di-calancoi%C3%A2-industrial-areas-of-porto-torres-and-landfill-of-calancoi-in-sardinia-region-southern
#2
Francesco Sgarangella, Giuseppe Bitti, Maria Caterina Suelzu, Pietro Desini, Patrizia Piras, Gianuario Fiori, Maurizio Cossu, Giannina Chessa
OBJECTIVES: to assess concentration levels of dioxins, PCBs, and chemical elements in sheep milk produced in areas close to the Italian contaminated site (CS) located in the North-West of Sardinia (Southern Italy), named «Aree industriali di Porto Torres e discarica di Calancoi» (i.e., industrial areas of Porto Torres and landfill of Calancoi). DESIGN: monitoring plan of environmental contaminants on zootechnical productions based on analysis of dioxins, PCBs, heavy metals (Cd, Hg, Pb), and other elements (As, Be, Cr, Ni, Tl , Zn) in sheep bulk milk...
November 2016: Epidemiologia e Prevenzione
https://www.readbyqxmd.com/read/27912754/bovine-mastitis-prevalence-risk-factors-and-isolation-of-staphylococcus-aureus-in-dairy-herds-at-hawassa-milk-shed-south-ethiopia
#3
Rahmeto Abebe, Hagere Hatiya, Mesele Abera, Bekele Megersa, Kassahun Asmare
BACKGROUND: Mastitis is a disease of major economic importance in dairy industry worldwide. It is of particular concern in developing countries like Ethiopia, where milk and milk products are scarce. The objectives of the study were to estimate the prevalence of mastitis, identify the cow-and herd-level potential risk factors and isolate Staphylococcus aureus, one of etiological agents for contagious mastitis, from cows positive for mastitis. A total of 529 lactating cows selected randomly from 95 herds were screened by California mastitis test (CMT) for sub-clinical mastitis...
December 3, 2016: BMC Veterinary Research
https://www.readbyqxmd.com/read/27890004/lactic-acid-bacteria-as-cell-factories-for-the-generation-of-bioactive-peptides
#4
Lucía Brown, Esteban Vera Pingitore, Fernanda Mozzi, Lucila Saavedra, Josefina M Villegas, Elvira M Hebert
There is a growing interest in the incorporation of functional foods in the daily diet to achieve health promotion and disease risk reduction. Numerous studies have focused on the production of biologically active peptides as nutraceuticals and functional food ingredients due to their health benefits. These short peptides, displaying antihypertensive, antioxidant, mineral binding, immunomodulatory and antimicrobial activities are hidden in a latent state within the primary sequences of food proteins requiring enzymatic proteolysis for their release...
November 23, 2016: Protein and Peptide Letters
https://www.readbyqxmd.com/read/27889146/the-potential-of-species-specific-tagatose-6-phosphate-t6p-pathway-in-lactobacillus-casei-group-for-galactose-reduction-in-fermented-dairy-foods
#5
Qinglong Wu, Nagendra P Shah
Residual lactose and galactose in fermented dairy foods leads to several industrial and health concerns. There is very little information pertaining to manufacture of fermented dairy foods that are low in lactose and galactose. In the present study, comparative genomic survey demonstrated the constant presence of chromosome-encoded tagatose-6-phosphate (T6P) pathway in Lactobacillus casei group. Lactose/galactose utilization tests and β-galactosidase assay suggest that PTS(Gal) system, PTS(Lac) system and T6P pathway are major contributors for lactose/galactose catabolism in this group of organisms...
April 2017: Food Microbiology
https://www.readbyqxmd.com/read/27889129/biofouling-of-ultrafiltration-membrane-by-dairy-fluids-characterization-of-pioneer-colonizer-bacteria-using-a-dna-metabarcoding-approach
#6
Julien Chamberland, Marie-Hélène Lessard, Alain Doyen, Steve Labrie, Yves Pouliot
Biofouling of filtration membranes is a major quality and performance issue for the dairy industry. Because biofilms that survive cleaning cycles become resistant over time, prevention strategies limiting the adhesion of bacteria to membranes should be prioritized for sustainable control of biofouling. However, this cannot be achieved because the pioneer bacteria colonizing these membranes are still unknown. Consequently, the objective of this study was to characterize pioneer bacteria on the filtration membrane surface and to measure the effect of filtration operational parameters on their diversity...
November 23, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27889124/assessing-genomic-prediction-accuracy-for-holstein-sires-using-bootstrap-aggregation-sampling-and-leave-one-out-cross-validation
#7
Ashley A Mikshowsky, Daniel Gianola, Kent A Weigel
Since the introduction of genome-enabled prediction for dairy cattle in 2009, genomic selection has markedly changed many aspects of the dairy genetics industry and enhanced the rate of response to selection for most economically important traits. Young dairy bulls are genotyped to obtain their genomic predicted transmitting ability (GPTA) and reliability (REL) values. These GPTA are a main factor in most purchasing, marketing, and culling decisions until bulls reach 5 yr of age and their milk-recorded offspring become available...
November 23, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27889122/genome-wide-association-study-for-cheese-yield-and-curd-nutrient-recovery-in-dairy-cows
#8
C Dadousis, S Biffani, C Cipolat-Gotet, E L Nicolazzi, G J M Rosa, D Gianola, A Rossoni, E Santus, G Bittante, A Cecchinato
Cheese production and consumption are increasing in many countries worldwide. As a result, interest has increased in strategies for genetic selection of individuals for technological traits of milk related to cheese yield (CY) in dairy cattle breeding. However, little is known about the genetic background of a cow's ability to produce cheese. Recently, a relatively large panel (1,264 cows) of different measures of individual cow CY and milk nutrient and energy recoveries in the cheese (REC) became available...
November 23, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27874095/longitudinal-metagenomic-profiling-of-bovine-milk-to-assess-the-impact-of-intramammary-treatment-using-a-third-generation-cephalosporin
#9
Erika K Ganda, Rafael S Bisinotto, Svetlana F Lima, Kristina Kronauer, Dean H Decter, Georgios Oikonomou, Ynte H Schukken, Rodrigo C Bicalho
Antimicrobial usage in food animals has a direct impact on human health, and approximately 80% of the antibiotics prescribed in the dairy industry are used to treat bovine mastitis. Here we provide a longitudinal description of the changes in the microbiome of milk that are associated with mastitis and antimicrobial therapy. Next-generation sequencing, 16 S rRNA gene quantitative real-time PCR, and aerobic culturing were applied to assess the effect of disease and antibiotic therapy on the milk microbiome...
November 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27873115/life-cycle-assessment-of-cheese-production-process-in-a-small-sized-dairy-industry-in-brazil
#10
Hudson Carlos Maia Santos, Henrique Leonardo Maranduba, José Adolfo de Almeida Neto, Luciano Brito Rodrigues
Current research identifies, analyzes, and suggests improvements for minimizing environmental impacts in the manufacture of cheese using the life cycle assessment. Data collection and development of the inventory were performed in a small-sized dairy industry in Brazil. A cradle-to-gate approach was conducted based on the primary data from cheese production and secondary data from databases. The ReCiPe method was used for the impact assessment, considering the categories climate change, ozone depletion, terrestrial acidification, freshwater eutrophication, photochemical oxidant formation, particulate matter formation, water depletion, and fossil depletion...
November 21, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27865511/modeling-genetic-and-nongenetic-variation-of-feed-efficiency-and-its-partial-relationships-between-component-traits-as-a-function-of-management-and-environmental-factors
#11
Y Lu, M J Vandehaar, D M Spurlock, K A Weigel, L E Armentano, C R Staples, E E Connor, Z Wang, M Coffey, R F Veerkamp, Y de Haas, R J Tempelman
Feed efficiency (FE), characterized as the fraction of feed nutrients converted into salable milk or meat, is of increasing economic importance in the dairy industry. We conjecture that FE is a complex trait whose variation and relationships or partial efficiencies (PE) involving the conversion of dry matter intake to milk energy and metabolic body weight may be highly heterogeneous across environments or management scenarios. In this study, a hierarchical Bayesian multivariate mixed model was proposed to jointly infer upon such heterogeneity at both genetic and nongenetic levels on PE and variance components (VC)...
November 16, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27865510/electrophoretic-characterization-of-protein-interactions-suggesting-limited-feasibility-of-accelerated-shelf-life-testing-of-ultra-high-temperature-milk
#12
Manpreet Kaur Grewal, Jayani Chandrapala, Osaana Donkor, Vasso Apostolopoulos, Todor Vasiljevic
Accelerated shelf-life testing is applied to a variety of products to estimate keeping quality over a short period of time. The industry has not been successful in applying this approach to ultra-high temperature (UHT) milk because of chemical and physical changes in the milk proteins that take place during processing and storage. We investigated these protein changes, applying accelerated shelf-life principles to UHT milk samples with different fat levels and using native- and sodium dodecyl sulfate-PAGE. Samples of UHT skim and whole milk were stored at 20, 30, 40, and 50°C for 28 d...
November 16, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27863096/outbreak-of-occupational-brucellosis-at-a-pharmaceutical-factory-in-southeast-china
#13
B D Zhan, S Q Wang, S M Lai, Y Lu, X G Shi, G P Cao, X L Hu, C J Zheng, Z Y Yu, J M Zhang, C F Fang, Z Y Gong
Brucellosis is an occupational disease affecting workers in butcher shops, the milking and dairy product industry, causing more than 500 000 new cases around the world. As a national statutory B infectious disease in China, morbidity of brucellosis is rapidly increasing in recent years. We report an occupational outbreak of brucellosis infection in a pharmaceutical factory. Exposure was a result of manual operation in the process line, close contact with sheep placentas, insufficient disinfection and repeated using of protective suits and infected by aerosol dissemination...
November 12, 2016: Zoonoses and Public Health
https://www.readbyqxmd.com/read/27847416/infrared-thermography-a-potential-noninvasive-tool-to-monitor-udder-health-status-in-dairy-cows
#14
REVIEW
M Sathiyabarathi, S Jeyakumar, A Manimaran, G Jayaprakash, Heartwin A Pushpadass, M Sivaram, K P Ramesha, D N Das, Mukund A Kataktalware, M Arul Prakash, R Dhinesh Kumar
The animal husbandry and livestock sectors play a major role in the rural economy, especially for the small and marginal farmers. India has the largest livestock population in the world and ranks first in the milk production. Mastitis is the most common and expensive infectious disease in dairy cattle. The global economic losses per year due to mastitis amounts to USD 35 billion and for Indian dairy industry ₹6000 crores per year. Early detection of mastitis is very important to reduce the economic loss to the dairy farmers and dairy industry...
October 2016: Veterinary World
https://www.readbyqxmd.com/read/27845025/effect-of-different-fat-replacers-on-the-physicochemical-and-instrumental-analysis-of-low-fat-cupuassu-goat-milk-yogurts
#15
Marion P Costa, Beatriz S Frasao, Bruna L Rodrigues, Adriana Co Silva, Carlos A Conte-Junior
The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP). Three batches of each cupuassu goat milk yogurt were prepared and analysed on the 1st day of storage by pH, proximate composition, colour, apparent viscosity, and texture. In comparison to yogurts from whole (W) or skimmed milk (S), all of the fat replacers improved the physicochemical properties (P < 0·05)...
November 2016: Journal of Dairy Research
https://www.readbyqxmd.com/read/27842963/environmental-impacts-of-milk-powder-and-butter-manufactured-in-the-republic-of-ireland
#16
William Finnegan, Jamie Goggins, Eoghan Clifford, Xinmin Zhan
The abolition of the milk quota system that was in place in Europe was abolished in 2015, which instigated an immediate increase in milk production in many European countries. This increase will aid in addressing the world's ever growing demand for food, but will incur increased stresses on the environmental impact and sustainability of the dairy industry. In this study, an environmental life cycle assessment was performed in order to estimate the environmental impacts associated with the manufacture of milk powder and butter in the Republic of Ireland...
November 11, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27830423/trans-fatty-acids-in-colostrum-mature-milk-and-diet-of-lactating-adolescents
#17
Roseli de Souza Santos da Costa, Flavia da Silva Santos, Daniela de Barros Mucci, Tânia Vignuda de Souza, Fátima Lucia de Carvalho Sardinha, Célia Regina Moutinho de Miranda Chaves, Maria das Graças Tavares do Carmo
The purpose of this study was to investigate the trans fatty acids (TFA) content and distribution in colostrum, mature milk, and diet of adolescent mothers, after TFA declaration in food labels became mandatory in Brazil. Participants were healthy adolescents (n 54, 15-19 years, 1-90 days postpartum) practicing exclusive breastfeeding. Milk samples were collected 3 days after delivery (colostrum) and in the third month postpartum (mature milk) by hand expression. The fatty acid composition of the milk samples was determined by gas chromatography...
November 9, 2016: Lipids
https://www.readbyqxmd.com/read/27827963/electrochemical-affinity-biosensors-based-on-disposable-screen-printed-electrodes-for-detection-of-food-allergens
#18
REVIEW
Alina Vasilescu, Gilvanda Nunes, Akhtar Hayat, Usman Latif, Jean-Louis Marty
Food allergens are proteins from nuts and tree nuts, fish, shellfish, wheat, soy, eggs or milk which trigger severe adverse reactions in the human body, involving IgE-type antibodies. Sensitive detection of allergens in a large variety of food matrices has become increasingly important considering the emergence of functional foods and new food manufacturing technologies. For example, proteins such as casein from milk or lysozyme and ovalbumin from eggs are sometimes used as fining agents in the wine industry...
November 5, 2016: Sensors
https://www.readbyqxmd.com/read/27825873/highly-sensitive-and-selective-erythromycin-nanosensor-employing-fiber-optic-spr-ery-imprinted-nanostructure-application-in-milk-and-honey
#19
Anand M Shrivastav, Sruthi P Usha, Banshi D Gupta
An erythromycin (ERY) detection method is proposed using the fiber optic core decorated with the coatings of silver and an over layer of ERY imprinted nanoparticles. Synthesis of ERY imprinted nanoparticles is carried out using miniemulsion method. The operating range of the sensor is observed to be from 1.62×10(-3) to 100µM while the sensor possesses the linear response for ERY concentration range from 0.1 to 5µM. The sensing method shows a maximum sensitivity of 205nm/µM near ERY concentration of 0.01µM...
October 19, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27822898/optimization-of-air-blast-drying-process-for-manufacturing-saccharomyces-cerevisiae-and-non-saccharomyces-yeast-as-industrial-wine-starters
#20
Sae-Byuk Lee, Won-Seok Choi, Hyun-Jung Jo, Soo-Hwan Yeo, Heui-Dong Park
Wine yeast (Saccharomyces cerevisiae D8) and non-Saccharomyces wine yeasts (Hanseniaspora uvarum S6 and Issatchenkia orientalis KMBL5774) were studied using air-blast drying instead of the conventional drying methods (such as freeze and spray drying). Skim milk-a widely used protective agent-was used and in all strains, the highest viabilities following air-blast drying were obtained using 10% skim milk. Four excipients (wheat flour, nuruk, artichoke powder, and lactomil) were evaluated as protective agents for yeast strains during air-blast drying...
December 2016: AMB Express
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