Michael J Gerdes, Christopher J Sevinsky, Anup Sood, Sudeshna Adak, Musodiq O Bello, Alexander Bordwell, Ali Can, Alex Corwin, Sean Dinn, Robert J Filkins, Denise Hollman, Vidya Kamath, Sireesha Kaanumalle, Kevin Kenny, Melinda Larsen, Michael Lazare, Qing Li, Christina Lowes, Colin C McCulloch, Elizabeth McDonough, Michael C Montalto, Zhengyu Pang, Jens Rittscher, Alberto Santamaria-Pang, Brion D Sarachan, Maximilian L Seel, Antti Seppo, Kashan Shaikh, Yunxia Sui, Jingyu Zhang, Fiona Ginty
Limitations on the number of unique protein and DNA molecules that can be characterized microscopically in a single tissue specimen impede advances in understanding the biological basis of health and disease. Here we present a multiplexed fluorescence microscopy method (MxIF) for quantitative, single-cell, and subcellular characterization of multiple analytes in formalin-fixed paraffin-embedded tissue. Chemical inactivation of fluorescent dyes after each image acquisition round allows reuse of common dyes in iterative staining and imaging cycles...
July 16, 2013: Proceedings of the National Academy of Sciences of the United States of America