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Autophagy and laryngeal carcinoma

Yunxiang Ji, Li Li, Qilei Tao, Xuyao Zhang, Jingyun Luan, Shuwei Zhao, Huanhai Liu, Dianwen Ju
Laryngeal squamous cell carcinoma (LSCC), one of the most common malignancies in the head and neck, has poor prognosis and high mortality. The need of novel and effective treatment for LSCC is urgent. Asparaginase, an enzyme-depriving asparagine, has been employed for the treatment of various cancers. In this study, we reported for the first time that asparaginase could induce remarkable cytotoxicity and caspase-dependent apoptosis in human LSCC Tu212 and Tu686 cells. Meanwhile, autophagy was triggered by asparaginase in LSCC cells, which was confirmed by accumulation of autophagosomes and the conversion of light chain 3-I (LC3-I) to LC3-II...
March 28, 2017: Applied Microbiology and Biotechnology
Xi-Jun Zhang, Shen-Shan Jia
Targeting cancer cells is crucial for improving the efficiency of laryngeal cancer treatment. However, the signaling pathway and therapeutic strategy, related to the tumor, still need further research. Dietary flavonoid fisetin (3,3',4',7-tetrahydroxyflavone) found in many fruits and vegetables has been shown in preclinical studies to inhibit cancer growth through regulating cell cycle, apoptosis, angiogenesis, invasion and metastasis without causing any toxicity to normal cells. PI3K/AKT and ERK1/2 have been known as essential signaling pathways to modulate cell proliferation, apoptosis as well as autophagy via mTOR, Caspase-3 and NF-κB signals...
October 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Min-Woo Baek, Heui-Seung Cho, Sun-Hun Kim, Won-Jae Kim, Ji-Yeon Jung
Ascorbic acid induces apoptosis, autophagy, and necrotic cell death in cancer cells. We investigated the mechanisms by which ascorbic acid induces death in laryngeal squamous cell carcinoma Hep2 cells. Ascorbic acid markedly reduced cell viability and induced death without caspase activation and an increase in cytochrome c. Hep2 cells exposed to ascorbic acid exhibited membrane rupture and swelling, the morphological characteristics of necrotic cell death. The generation of reactive oxygen species (ROS) was increased in Hep2 cells treated with ascorbic acid, and pretreatment with N-acetylcysteine blocked ascorbic acid-induced cell death...
February 2017: Journal of Cellular Physiology
Chen Lin, Ziyu Wang, Li Li, Yong He, Jiajun Fan, Zhongyu Liu, Shuwei Zhao, Dianwen Ju
Laryngeal squamous cell carcinoma (LSCC), one of the most common malignant solid tumors in the world, has a high rate of mortality, recurrence, and metastasis. Recombinant human arginase (rhArg) recently has been developed in arginine deprivation therapy for a number of malignant tumors. In the present study, we observed that rhArg triggered significant cytotoxicity in human laryngeal squamous cell carcinoma Tu212 cells. Meanwhile, we observed that rhArg simultaneously activated autophagic flux in Tu212 cells, which was demonstrated by the accumulation of autophagosome and light chain 3 II (LC3-II)...
October 2015: Applied Microbiology and Biotechnology
Débora Lima Pereira, Ana Carolina Dos Santos Ferreira, Giselle Pinto de Faria, Jolie Kiemlian Kwee
Laryngeal squamous cell carcinoma (LSCC) is the most common among several types of head and neck cancers. Current treatments have a poor effect on early and advanced cases, and further investigations for novel agents against LSCCs are desirable. In this study, we elucidate the cytotoxic enhancing effect of arsenic trioxide (As2O3) combined with L-buthionine sulfoximine (BSO) in LSCC. The effect of BSO with As2O3 or Cisplatin (CDDP) on cell viability was examined using 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)...
January 2015: Pathology Oncology Research: POR
Amal Kamal Abdel-Aziz, Samia Shouman, Ebtehal El-Demerdash, Mohamed Elgendy, Ashraf B Abdel-Naim
Tyrosine kinases play a pivotal role in oncogenesis. Although tyrosine kinase inhibitors as sunitinib malate are used in cancer therapy, emerging studies report compromised cytotoxicity when used as monotherapy and thus combinations with other anti-cancer agents is recommended. Chloroquine is a clinically available anti-malarial agent which has been shown to exhibit anti-cancer activity. In the current study, we questioned whether chloroquine can modulate sunitinib cytotoxicity. We found that chloroquine synergistically augmented sunitinib cytotoxicity on human breast (MCF-7 and T-47D), cervical (Hela), colorectal (Caco-2 and HCT116), hepatocellular (HepG2), laryngeal (HEp-2) and prostate (PC3) cancer cell lines as indicated by combination and concentration reduction indices...
June 25, 2014: Chemico-biological Interactions
Dorota Bądziul, Joanna Jakubowicz-Gil, Roman Paduch, Kazimierz Głowniak, Antoni Gawron
The aim of the present study was to assess the effect of quercetin and imperatorin administered separately and in combination on apoptosis and autophagy induction in human cervical carcinoma HeLa cells and laryngeal carcinoma Hep-2 cells cultured in vitro. Conducted MTT measurements proved that quercetin and imperatorin displayed a strong antiproliferative activity manifested in markedly reduction of HeLa and Hep-2 cells viability as a result of treatment with 50 μM of each compound. Further cell staining assays revealed that concentration mentioned above generated the highest percentage of apoptotic cells especially in the case of application of both drugs for 48 h...
July 2014: Molecular and Cellular Biochemistry
Lijing Zhao, Bo Teng, Lianji Wen, Qingjie Feng, Hebin Wang, Na Li, Yafang Wang, Zuowen Liang
The mammalian target of rapamycin (mTOR) kinase forms two multiprotein complexes, mTORC1 and mTORC2, which regulate cell growth, survival, and autophagy. Allosteric inhibitors of mTORC1, such as rapamycin, have been extensively used to study tumor cell growth, proliferation, and autophagy but have shown only limited clinical utility. Here, we describe AZD8055, a novel ATP-competitive inhibitor of mTOR kinase activity, against all class I phosphatidylinositol3-kinase (PI3K) and other members of the PI3K-like kinase family...
2014: International Journal of Clinical and Experimental Medicine
Li Huang, Shuang Wang, Shi-Sheng Li, Xin-Ming Yang
Beclin-1 plays a critical role in the regulation of autophagy, apoptosis, differentiation and the development and progression of cancer. The aim of the present investigation was to analyze the Beclin-1 protein expression and to assess its prognostic significance in tissue of laryngeal squamous cell carcinoma (LSCC). Beclin-1 protein expression in 82 primary laryngeal squamous cell carcinoma and 40 paracarcinoma non-tumor tissue samples was analyzed by immunohistochemistry and correlated with clinicopathological parameters and patients' outcome...
October 2013: Pathology Oncology Research: POR
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