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Manar M Ahmed, Khaled M Aboshanab, Yasser M Ragab, Dominique M Missiakas, Khaled A Aly
The ESAT-6-like secretion system (ESS) of Staphylococcus aureus plays a significant role in persistent infections. EssB is a highly conserved bitopic ESS protein comprising a cytosolic N-terminus, single transmembrane helix and a C-terminus located on the trans-side of the membrane. Six systematic truncations covering various domains of EssB were constructed, followed by bacterial two-hybrid screening of their interaction with EsaA, another conserved integral membrane component of the ESS pathway. Results show that the transmembrane domain of EssB is critical for heterodimerization with EsaA...
May 8, 2018: Archives of Microbiology
Jun Wang, Changyang Zhong, Yan Zhang, Yingnan Wei, Huili Liu, Chunli Wu, Yongxing Yan
Objective Endovascular stent-assistant angioplasty (ESAA) is a valid treatment for symptomatic vertebrobasilar artery stenosis (SVAS), but the long-term effect and the improvement of condition compared with medication treatment are unknown. This study investigated the long-term efficacy of ESAA in patients with moderate and severe SVAS, and compared the efficacy with medication treatment. Materials and methods We conducted a retrospective analysis of clinical data of 43 patients with moderate and severe SVAS hospitalized in our department...
February 2018: Interventional Neuroradiology
Khaled A Aly, Mark Anderson, Ryan Jay Ohr, Dominique Missiakas
The ESAT6-like secretion system (ESS) of Staphylococcus aureus promotes effector protein transport across the bacterial envelope. Genes in the ESS cluster are required for S. aureus establishment of persistent abscess lesions and the modulation of immune responses during bloodstream infections. However, the biochemical functions of most of the ESS gene products, specifically the identity of secretion machine components, are unknown. Earlier work demonstrated that deletion of essB , which encodes a membrane protein, abolishes S...
December 1, 2017: Journal of Bacteriology
Anusak Kerdsin, Rujirat Hatrongjit, Shigeyuki Hamada, Yukihiro Akeda, Marcelo Gottschalk
A multiplex polymerase chain reaction (mPCR) was developed for simultaneous detection (single reaction) of genes specific to five frequent clinically relevant β-hemolytic streptococcal species: Streptococcus pyogenes (Spy1258), Streptococcus agalactiae (cfb and cpn60), Streptococcus dysgalactiae subsp. equisimilis (16S-23S intergenic spacer) , S. equi subsp. zooepidemicus (esaA and sorD), and Streptococcus anginosus group (moaC). No cross-reaction was observed with other bacterial species. This test was validated and successfully used with 725 clinical isolates involved in pathological conditions in Thailand and collected between March 2014 and December 2015...
December 2017: Molecular and Cellular Probes
Youichi Yasui, Charles P Hannon, Dexter Seow, John G Kennedy
Ankle arthrodesis is a common treatment used for patients with end-stage ankle arthritis (ESAA). The surgical goal of ankle arthrodesis is to obtain bony union between the tibia and talus with adequate alignment [slight valgus (0°-5°)], neutral dorsiflexion, and slight external rotation positions) in order to provide a pain-free plantigrade foot for weightbearing activities. There are many variations in operative technique including deferring approaches (open or arthroscopic) and differing fixation methods (internal or external fixation)...
November 18, 2016: World Journal of Orthopedics
Gerardo P García-García, Neyla C Esaa-Caride, Nancy D Jurado-Guano, Luis Muñoz-Bellido
PURPOSE: To evaluate the medical and surgical management and treatment of ocular injuries with chestnut burr recorded at the Hospital El Bierzo between 2009 and 2015. METHODS: A prospective study of ocular injuries with chestnut burr was conducted for the period from 2009 to 2015. Patients were detected in the Emergency Department and referred to the Ophthalmology Department for study and medical and surgical treatment. Ten eyes were evaluated in 10 patients. The injuries ranged from ocular contusion without corneal involvement to trauma with corneal perforation caused by spines...
October 2016: Cornea
Martin McPhillie, Ying Zhou, Kamal El Bissati, Jitender Dubey, Hernan Lorenzi, Michael Capper, Amanda K Lukens, Mark Hickman, Stephen Muench, Shiv Kumar Verma, Christopher R Weber, Kelsey Wheeler, James Gordon, Justin Sanders, Hong Moulton, Kai Wang, Taek-Kyun Kim, Yuqing He, Tatiana Santos, Stuart Woods, Patty Lee, David Donkin, Eric Kim, Laura Fraczek, Joseph Lykins, Farida Esaa, Fatima Alibana-Clouser, Sarah Dovgin, Louis Weiss, Gael Brasseur, Dyann Wirth, Michael Kent, Leroy Hood, Brigitte Meunieur, Craig W Roberts, S Samar Hasnain, Svetlana V Antonyuk, Colin Fishwick, Rima McLeod
Toxoplasma gondii, the most common parasitic infection of human brain and eye, persists across lifetimes, can progressively damage sight, and is currently incurable. New, curative medicines are needed urgently. Herein, we develop novel models to facilitate drug development: EGS strain T. gondii forms cysts in vitro that induce oocysts in cats, the gold standard criterion for cysts. These cysts highly express cytochrome b. Using these models, we envisioned, and then created, novel 4-(1H)-quinolone scaffolds that target the cytochrome bc1 complex Qi site, of which, a substituted 5,6,7,8-tetrahydroquinolin-4-one inhibits active infection (IC50, 30 nM) and cysts (IC50, 4 μM) in vitro, and in vivo (25 mg/kg), and drug resistant Plasmodium falciparum (IC50, <30 nM), with clinically relevant synergy...
July 14, 2016: Scientific Reports
Jennifer J Crawford, Jeff K Vallance, Nicholas L Holt, Kerry S Courneya
OBJECTIVE: We examined the demographic, medical and behavioral correlates of participation and interest in extreme sport/adventure activities (ESAA) in gynecologic cancer survivors. METHODS: A random sample of 621 gynecologic cancer survivors in Alberta, Canada, completed a mailed self-report questionnaire assessing medical, demographic, and behavioral variables and participation and interest in ESAA. RESULTS: Multivariate analyses revealed that gynecologic cancer survivors were more likely to participate in ESAA if they met aerobic exercise guidelines (OR=1...
March 2016: American Journal of Health Behavior
Marta J Piercey, Patricia A Hingston, Lisbeth Truelstrup Hansen
Listeria monocytogenes is a pathogenic foodborne bacterium whose persistence in food processing environments is in part attributed to its biofilm formation. Most biofilm studies have been carried out at 30-37 °C rather than at temperatures found in the food processing plants (i.e., 10-20 °C). The objective of the present study was to mine for novel genes that contribute to L. monocytogenes biofilm formation at 15 °C using the random insertional mutagenesis approach. A library of 11,024 L. monocytogenes 568 (serotype 1/2a) Himar1 insertional mutants was created...
April 16, 2016: International Journal of Food Microbiology
Masoome Khalife, Dina Morshedi, Farhang Aliakbari, Amir Tayaranian Marvian, Hossein Mohammad Beigi, Sadegh Azimzadeh Jamalkandi, Francisco Pan-Montojo
Aggregated alpha-synuclein (α-SYN) is the major component of Lewy bodies and Lewy neurites, two of the pathological hallmarks of Parkinson's disease (PD). Aggregation of α-SYN leads to toxic species involved in the degeneration of dopaminergic neurons in the midbrain. Different studies suggest a strong association between the presence of dopamine (DA) and the cell specific degeneration caused by α-SYN aggregates in PD. Despite extensive studies on the effect of DA on α-SYN fibrillation, it remains unclear how the simultaneous presence of DA and α-SYN influences the degeneration of dopaminergic neurons...
August 2015: Protein Journal
Holger Kneuper, Zhen Ping Cao, Kate B Twomey, Martin Zoltner, Franziska Jäger, James S Cargill, James Chalmers, Magdalena M van der Kooi-Pol, Jan Maarten van Dijl, Robert P Ryan, William N Hunter, Tracy Palmer
The Type VII protein secretion system, found in Gram-positive bacteria, secretes small proteins, containing a conserved W-x-G amino acid sequence motif, to the growth medium. Staphylococcus aureus has a conserved Type VII secretion system, termed Ess, which is dispensable for laboratory growth but required for virulence. In this study we show that there are unexpected differences in the organization of the ess gene cluster between closely related strains of S. aureus. We further show that in laboratory growth medium different strains of S...
September 2014: Molecular Microbiology
Liliana Rivera-Espinosa, Esaú Floriano-Sánchez, José Pedraza-Chaverrí, Elvia Coballase-Urrutia, Aristides Iii Sampieri, Daniel Ortega-Cuellar, Noemí Cárdenas-Rodríguez, Liliana Carmona-Aparicio
Hepatic encephalopathy (HE) is a common complication of cirrhosis, of largely reversible impairment of brain function occurring in patients with acute or chronic liver failure or when the liver is bypassed by portosystemic shunts. The mechanisms causing this brain dysfunction are still largely unclear. The need to avoid complications caused by late diagnosis has attracted interest to understand the mechanisms underlying neuronal damage in order to find markers that will allow timely diagnosis and to propose new therapeutic alternatives to improve the care of patients...
2013: International Journal of Molecular Sciences
Adolfo Daniel Rodríguez-Carrizalez, José Alberto Castellanos-González, Esaú César Martínez-Romero, Guillermo Miller-Arrevillaga, David Villa-Hernández, Pedro Pablo Hernández-Godínez, Genaro Gabriel Ortiz, Fermín Paul Pacheco-Moisés, Ernesto Germán Cardona-Muñoz, Alejandra Guillermina Miranda-Díaz
BACKGROUND: Diabetic retinopathy (DR) is a preventable cause of visual disability. The aims of the present study were to investigate levels and behavior oxidative stress markers and mitochondrial function in non-proliferative DR (NPDR) and to establish the correlation between the severity of NPDR and markers of oxidative stress and mitochondrial function. METHODS: In a transverse analysis, type 2 diabetes mellitus (T2DM) patients with mild, moderate and severe non-proliferative DR (NPDR) were evaluated for markers of oxidative stress (i...
March 2014: Journal of Diabetes
Shanmiao Gou, Pengfei Cui, Xiangsheng Li, Pengfei Shi, Tao Liu, Chunyou Wang
Pancreatic cancer is the fourth leading cause of cancer related deaths in the United States. The prognosis remains dismal with little advance in treatment. Metformin is a drug widely used for the treatment of type II diabetes. Recent epidemiologic data revealed that oral administration of metformin is associated with a reduced risk of pancreatic cancer, suggesting its potential as a novel drug for this disease. Many studies have demonstrated the in vitro anticancer action of metformin, but the typically used concentrations were much higher than the in vivo plasma and tissue concentrations achieved with recommended therapeutic doses of metformin, and low concentrations of metformin had little effect on the proliferation of pancreatic cancer cells...
2013: PloS One
Rocío Contreras Sánchez-Matamoros, Antonio M Gil Serrano, Pilar Tejero-Mateo, Javier Ollero, Esaú Megías Saavedra, Miguel A Rodríguez-Carvajal
The lipopolysaccharide of a Gram-negative bacterium having a putative plant-growth promoting activity (Pantoea ananatis AEP17) has been isolated and subjected to partial hydrolysis. The O-antigen has been studied by mass spectrometry and NMR experiments. On the basis of these experiments it is concluded that the following repeating unit is present in the polysaccharide: →3)-β-d-GlcpNAc-(1→3)[α-d-GalpAN-(1→2)]-α-l-Rhap-(1→2)-α-l-Rhap-(1→3)-α-l-Rhap-(1→2)-α-l-Rhap-(1→ The occurrence of d-galacturonamide (GalAN) is unusual in bacterial O-polysaccharides...
March 22, 2013: Carbohydrate Research
Moisés Rubio-Osornio, Sergio Montes, Yessica Heras-Romero, Jorge Guevara, Carmen Rubio, Penélope Aguilera, Susana Rivera-Mancia, Esaú Floriano-Sánchez, Antonio Monroy-Noyola, Camilo Ríos
Parkinson's disease (PD) is a neurodegenerative disorder characterized by decreased dopamine, intracellular inclusions (Lewy bodies) and brain iron deposits. PD has also been related with reduced ferroxidase activity, diminished antioxidant defenses and lipid peroxidation. Striatal injection of 1-methyl-4-phenylpyridinium (MPP(+)) into rodents reproduces the major biochemical characteristics of PD, including oxidative stress. Copper (Cu) plays an important role as prosthetic group of several proteins involved in iron metabolism and antioxidant responses, such as ceruloplasmin (Cp)...
March 2013: Neuroscience Research
Esaú Joao, Maria I Gouvea, Laura Freimanis-Hance, Rachel A Cohen, Jennifer S Read, Victor Melo, Geraldo Duarte, Silvina Ivalo, Daisy M Machado, Jose Pilotto, George K Siberry
OBJECTIVE: To describe Group B Streptococcus (GBS) prevention policies at 12 Latin American sites participating in the NICHD (Eunice Kennedy Shriver National Institute of Child Health and Human Development) International Site Development Initiative (NISDI) Longitudinal Study in Latin American Countries (LILAC) and to determine rates of rectovaginal colonization and GBS-related disease among HIV-infected pregnant women and their infants. METHODS: Site surveys were used to assess prevention policies and practices administered cross-sectionally during 2010...
February 2013: International Journal of Gynaecology and Obstetrics
Alexandra A Soukup, Yi-Ming Chiang, Jin Woo Bok, Yazmid Reyes-Dominguez, Berl R Oakley, Clay C C Wang, Joseph Strauss, Nancy P Keller
Regulation of secondary metabolite (SM) gene clusters in Aspergillus nidulans has been shown to occur through cluster-specific transcription factors or through global regulators of chromatin structure such as histone methyltransferases, histone deacetylases, or the putative methyltransferase LaeA. A multicopy suppressor screen for genes capable of returning SM production to the SM deficient ΔlaeA mutant resulted in identification of the essential histone acetyltransferase EsaA, able to complement an esa1 deletion in Saccharomyces cereviseae...
October 2012: Molecular Microbiology
Dulce M Arias, Ana L Albarrán-Lara, Antonio González-Rodríguez, Juan Peñaloza-Ramírez, Oscar Dorado, Esaú Leyva
The tropical dry forest is a greatly endangered ecosystem, from which Jacaratia mexicana is a native tree. With the aim to assess the levels of genetic variation and population structure, four wild populations of J. mexicana were studied in the Sierra de Huautla Biosphere Reserve, Morelos, Mexico. For this, DNA was extracted from 159 individuals and were amplified with six random primers using the Random Amplified Polymorphic DNA (RAPD). A total of 54 bands were obtained, of which 50 (92.6%) were polymorphic...
March 2012: Revista de Biología Tropical
Leonardo Ponce da Motta, Márcio Araújo da Costa, Marcelo Barreto Gouvea, Alberto Saraiva Tibúrcio, Esaú Custódio João Filho, Maurício Specterow, Beatriz Cappatto da Silva, Maria Letícia Fernandes Oliveira Nascimento, Natasha Sbragio Ganem, Marcelo Rosandiski Lyra
Described here is a case of postmalaria neurological syndrome in a patient who presented infection by Plasmodium falciparum two months earlier. The patient received empiric use of acyclovir for herpetic meningoencephalitis, but neuropsychiatric symptoms improved only after administration of methylprednisolone.
November 2011: Revista da Sociedade Brasileira de Medicina Tropical
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