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Candida tropicalis

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https://www.readbyqxmd.com/read/29157206/antifungal-susceptibility-testing-of-candida-species-isolated-from-the-immunocompromised-patients-admitted-to-ten-university-hospitals-in-iran-comparison-of-colonizing-and-infecting-isolates
#1
Parisa Badiee, Hamid Badali, Teun Boekhout, Kambiz Diba, Abdolkarim Ghadimi Moghadam, Ali Hossaini Nasab, Hadis Jafarian, Rasoul Mohammadi, Hossein Mirhendi, Mohammad Javad Najafzadeh, Ahmad Shamsizadeh, Jafar Soltani
BACKGROUND: Antifungal susceptibility testing is a subject of interest in the field of medical mycology. The aim of the present study were the distributions and antifungal susceptibility patterns of various Candida species isolated from colonized and infected immunocompromised patients admitted to ten university hospitals in Iran. METHODS: In totally, 846 Candida species were isolated from more than 4000 clinical samples and identified by the API 20 C AUX system...
November 21, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29154198/evaluation-of-antioxidant-dna-targeting-antimicrobial-and-cytotoxic-studies-of-imine-capped-copper-and-nickel-nanoparticles
#2
P Adwin Jose, J Dhaveethu Raja, M Sankarganesh, J Rajesh
In this work, we have synthesized pyrimidine derivatives of Schiff base ligand 2-(4,6-dimethoxypyrimidin-2-ylimino)methyl)-6-methoxyphenol (DPMM) stabilized copper nanoparticles (DPMM-CuNPs) and nickel nanoparticles (DPMM-NiNPs) by modified Brust-Schiffrin technique as two step phase transfer assisted method and confirmed by UV-Visible, SEM and TEM analysis. The free radical scavenging activity of DPMM, DPMM-CuNPs &DPMM-NiNPs with 2, 2'-diphenylpicryl hydrazyl (DPPH), hydrogen peroxide (H2O2) super oxide dismutase (SOD) and nitric oxide (NO) shows that the antioxidant activity of DPMM-CuNPs is higher than DPMM &DPMM-NiNPs...
November 6, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29154013/the-potential-of-the-newly-isolated-thermotolerant-yeast-pichia-kudriavzevii-rz8-1-for-high-temperature-ethanol-production
#3
Nuttaporn Chamnipa, Sudarat Thanonkeo, Preekamol Klanrit, Pornthap Thanonkeo
High potential, thermotolerant, ethanol-producing yeasts were successfully isolated in this study. Based on molecular identification and phylogenetic analysis, the isolated thermotolerant yeasts were clustered in the genera of Pichia kudriavzevii, Candida tropicalis, Candida orthopsilosis, Candida glabrata and Kodamea ohmeri. A comparative study of ethanol production using 160g/L glucose as a substrate revealed several yeast strains that could produce high ethanol concentrations at high temperatures. When sugarcane bagasse (SCB) hydrolysate containing 85g/L glucose was used as a substrate, the yeast strain designated P...
November 4, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29142403/flavonoidal-constituents-antioxidant-antimicrobial-and-cytotoxic-activities-of-dipterygium-glaucum-grown-in-kingdom-of-saudi-arabia
#4
Usama Shaheen, Nagwa Abdelkader Shoeib, Abeer Temraz, Mohamed I S Abdelhady
Background: Dipterygium glaucum Decne. herb is one of the common traditional plants with multiple medicinal uses. Objective: To isolate the major constituents and to investigate the antioxidant, antimicrobial, and cytotoxic activities of this herb. Materials and Methods: Methanolic extract of D. glaucum herb was fractionated using n-hexane, dichloromethane, and n-butanol. Butanol fraction was chromatographed using column chromatography and preparative thin layer chromatography to isolate the major constituents...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29132935/risk-factors-and-outcomes-of-candidemia-caused-by-candida-parapsilosis-complex-in-a-medical-center-in-northern-taiwan
#5
Yen-Mu Wu, Po-Yen Huang, Jang-Jih Lu, Shian-Sen Shie, Jung-Jr Ye, Ting-Shu Wu, Ching-Tai Huang
To investigate the risk factors and outcomes associated with Candida parapsilosis candidemia, a retrospective study was conducted at a tertiary medical center in northern Taiwan. Patients with C. parapsilosis candidemia and corresponding controls with C. albicans candidemia were chosen and their demographics, comorbidities, risk factors, and clinical outcomes were reviewed. Antifungal susceptibility tests were performed using the Sensititre YeastOne colorimetric system. Matrix-assisted laser desorption ionization-time of flight mass spectrometry was used to classify the genomic species...
October 6, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29131083/the-effect-of-nutritive-and-non-nutritive-sweeteners-on-the-growth-adhesion-and-biofilm-formation-of-candida-albicans-and-candida-tropicalis
#6
Manjula M Weerasekera, Thilini A Jayarathna, Gayan K Wijesinghe, Chinthika P Gunasekara, Neluka Fernando, Nilwala Kottegoda, Lakshman P Samaranayake
OBJECTIVE: To determine the effect of glucose, sucrose, and saccharin on growth, adhesion and biofilm formation of Candida albicans and C.tropicalis. MATERIALS AND METHODS: The growth rates of mono-cultures of planktonic C. albicans and C. tropicalis and co-cultures (1:1), were determined in yeast nitrogen broth supplemented with 5% (30 mM) and 10% (60 mM) of glucose, sucrose, and saccharin, using optical density measurements at 2h intervals over a 14h period. Adhesion and biofilm growth were performed and the growth quantified using standard 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay...
November 1, 2017: Medical Principles and Practice: International Journal of the Kuwait University, Health Science Centre
https://www.readbyqxmd.com/read/29126627/-clinical-and-mycological-characteristics-of-onychomycosis-due-to-candida-at-institut-pasteur-of-c%C3%A3-te-d-ivoire
#7
K E Angora, A Ira-Bonouman, A H Vanga-Bosson, A Konaté, F K Kassi, K Tuo, K Sylla-Thanon, P C Kiki-Barro, S Miezan, A V Bedia-Tanoh, V Djohan, W Yavo, E H Menan, A T Offianan
OBJECTIVE: This study aims to study the clinical and mycological characteristics of onychomycosis due to Candida in mycology unit of Institut Pasteur of Côte d'Ivoire. PATIENTS AND METHODS: This is a retrospective study which was carried out on patients from 1990 to 2016 for mycological diagnosis of onychomycosis and which socio-demographic characteristics, direct examination and culture results were recorded. RESULTS: In this study, 1898 patient files were selected...
November 7, 2017: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29110510/%C3%AE-irradiation-from-radiotherapy-improves-the-virulence-potential-of-candida-tropicalis
#8
Eliane M da Silva, Brenda Kischkel, Cristiane S Shinobu-Mesquita, Patrícia S Bonfim-Mendonça, Elaine Sb Mansano, Marcos A da Silva, Jéssica F Barbosa, Adriana Fiorini, Luzmarina Hernandes, Marcia C Furlaneto, Terezinha Ie Svidzinski
AIM: To evaluate if radiation used in radiotherapy can cause changes in the virulence potential of Candida tropicalis ATCC 750. MATERIALS & METHODS: C. tropicalis was exposed in vitro to identical dose and scheme of irradiation would be used in patients with head and neck cancer. Some virulence parameters were analyzed before and after irradiation. RESULTS: Colony morphologies were irreversibly affected by irradiation. Increase in growth rate, filamentation, adhesion on cell lines and phagocytosis process were also observed...
November 7, 2017: Future Microbiology
https://www.readbyqxmd.com/read/29110155/streptomyces-swartbergensis-sp-nov-a-novel-tyrosinase-and-antibiotic-producing-actinobacterium
#9
Marilize le Roes-Hill, Alaric Prins, Paul R Meyers
As part of an antibiotic screening program, an actinobacterium, strain HMC13(T), was isolated from soil collected from the banks of the Gamka River, Western Cape Province, South Africa. The isolate was found to produce branched mycelia that differentiated into spiral spore chains with spiny spores. 16S rRNA gene sequence analysis showed the strain to be closely related to Streptomyces caelestis NRRL 2418(T) (99.72%) and Streptomyces azureus NBRC 12744(T) (99.51%). Chemotaxonomic analyses confirmed the classification of the strain as a member of the genus Streptomyces: LL-DAP in the peptidoglycan, no diagnostic sugars in the whole cell sugar pattern, dominant menaquinones including MK9(H8), MK9(H6), and the polar lipids detected included phosphatidylethanolamine...
November 6, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/29102310/-vertebral-osteomyelitis-due-to-candida-tropicalis-secondary-to-candidemia-a-case-report
#10
S Zayet, I Chaabane, B Kilani, M Bouaziz, F Ladeb, H B Tiouiri
Candida spp. vertebral osteomyelitis is rare. Clinical presentation is unspecific. Diagnosis requires mycological culture of a biopsy specimen. Therapeutic management is based on prolonged course of azole or liposomal amphotericin B. We report the case of Candida tropicalis vertebral osteomyelitis with epidural involvement in a 27 years-old male patient, followed for S-β-thalassemia and with a history of candidemia. The fungus was isolated from a needle biopsy of the vertebral disk. The outcome was favorable under antifungal treatment by amphotericin B and voriconazole...
October 25, 2017: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29101060/dispersal-of-single-and-mixed-non-albicans-candida-species-biofilms-by-%C3%AE-1-3-glucanase-in-vitro
#11
Yulong Tan, Matthias Leonhard, Su Ma, Doris Moser, Berit Schneider-Stickler
β-1,3-glucan plays a role in non-albicans Candida species biofilm formation and survival of biofilm Candida to stresses. In this study, we evaluated the antibiofilm activity of β-1,3-glucanase, which can degrade poly-β(1 → 3)-glucose of non-albicans Candida species biofilms, on single and mixed species biofilm of non-albicans Candida species, including Candida tropicalis, Candida parapsilosis and Candida krusei. Biofilm by all tested species in microplate were dispersed more than 60%. β-1,3-glucanase also detached mixed species biofilm in microplate and on medical material surface...
November 1, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29099103/detecci%C3%A3-n-molecular-de-especies-de-candida-en-espec%C3%A3-menes-de-pacientes-hospitalizados
#12
José Luis Camacho-Cardoso, María Ángeles Martínez-Rivera, Patricia Manzano-Gayosso, Luis Javier Méndez-Tovar, Rubén López-Martínez, Francisca Hernández-Hernández
Objective: To identify the most frequent Candida species in specimens from patients hospitalized in different medical centers of Mexico City, with suspected fungal infection. Methods: Specimens were grown on Sabouraud dextrose agar at 28°C for 72 h. In addition, DNA was extracted. Isolates were grown on CHROMagar Candida™, at 37°C for 48 h. The molecular identification was performed by polymerase chain reaction (PCR) using primers specific for four species. Results: Eighty one specimens were processed and included: bronchial lavage, pleural, cerebrospinal, peritoneal, ascites and bile fluids; blood, sputum, bone marrow, oro-tracheal cannula and ganglion...
2017: Gaceta Médica de México
https://www.readbyqxmd.com/read/29096684/antifungal-susceptibility-and-phenotypic-virulence-markers-of-candida-species-isolated-from-nepal
#13
Supram Hosuru Subramanya, Bharat Prasad Baral, Nawal Kishor Sharan, Niranjan Nayak, Yang Metok, Brijesh Sathian, Indira Bairy, Shishir Gokhale
OBJECTIVE: Candida species are part of the commensal microflora in many anatomical sites of the human body; however, breach in the integrity of the body part and impaired immunity of the host can lead to invasive candidiasis. A number of virulence determinants could contribute towards its pathogenicity. Thus we attempted to evaluate the in vitro expression of different virulence factors among clinical isolates of Candida species and assayed their susceptibility patterns against a range of antifungal agents...
November 2, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/29081766/an-update-on-candida-tropicalis-based-on-basic-and-clinical-approaches
#14
REVIEW
Diana L Zuza-Alves, Walicyranison P Silva-Rocha, Guilherme M Chaves
Candida tropicalis has emerged as one of the most important Candida species. It has been widely considered the second most virulent Candida species, only preceded by C. albicans. Besides, this species has been recognized as a very strong biofilm producer, surpassing C. albicans in most of the studies. In addition, it produces a wide range of other virulence factors, including: adhesion to buccal epithelial and endothelial cells; the secretion of lytic enzymes, such as proteinases, phospholipases, and hemolysins, bud-to-hyphae transition (also called morphogenesis) and the phenomenon called phenotypic switching...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29074432/healthcare-workers-hands-as-a-vehicle-for-the-transmission-of-virulent-strains-of-candida-spp-a-virulence-factor-approach
#15
Karina Mayumi Sakita, Daniella Renata Faria, Eliane Martins da Silva, Flávia Kelly Tobaldini-Valério, Erika Seki Kioshima, Terezinha Inez Estivalet Svidzinski, Patricia de Souza Bonfim-Mendonça
BACKGROUND: Although the majority of Candida infections are thought to come from endogenous sources, the healthcare workers' (HCWs) hands are being increasingly reported as vehicles for the transmission of pathogens. The aim of the present study was to evaluate the susceptibility of yeast isolated from the HCWs' hands and ICU (Intensive Care Unit) surfaces to antifungal agents and to determine the virulence potential and the genetic similarity between the same. METHODS: The susceptibility of yeasts from the HCWs' hands (n = 57) and ICU surfaces (n = 98) to conventional antifungals (fluconazole, voriconazole, amphotericin B and micafungin) was evaluated using the broth microdilution assay accordance with CLSI M27-A3...
October 23, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29062326/isolation-and-evaluation-of-candida-species-and-their-association-with-cd4-t-cells-counts-in-hiv-patients-with-diarrhoea
#16
Ayobami Awoyeni, Olarinde Olaniran, Babatunde Odetoyin, Rachel Hassan-Olajokun, Bolatito Olopade, David Afolayan, Oluwakayode Adekunle
BACKGROUND: Gastrointestinal infection is one of the most common infections among HIV patients. Candida spp have been implicated in the aetiology of chronic diarrhoea in HIV patients, but little is known about this in Nigeria. OBJECTIVES: We determined the prevalence of faecal candidiasis in HIV patients in relation to diarrhoea, CD4 counts, and other socio-demographic factors and the spectrum of Candida isolates involved. METHODS: One hundred and fifty four HIV patients were investigated...
June 2017: African Health Sciences
https://www.readbyqxmd.com/read/29062248/evaluation-of-candida-species-and-antifungal-susceptibilities-among-children-with-invasive-candidiasis
#17
Murat Sütçü, Manolya Acar, Gonca Erköse Genç, İlknur Kökçü, Hacer Aktürk, Gürkan Atay, Selda Hançerli Törun, Nuran Salman, Zayre Erturan, Ayper Somer
AIM: Non-albicans Candida species and resistant microorganisms have been more commonly isolated in invasive candidiasis in recent years. The aim of this study was to evaluate the distrubution of Candida spp and antifungal resistance in our clinic. MATERIAL AND METHODS: Fifty-four Candida isolates and antifungal susceptibility results obtained from patients diagnosed as having invasive candidiasis between December 2012 and June 2016 were included. Clinical and laboratory data were retrospectively analyzed...
September 2017: Türk Pediatri Arşivi
https://www.readbyqxmd.com/read/29062119/effects-of-glucose-concentration-on-1-18-cis-octadec-9-enedioic-acid-biotransformation-efficiency-and-lipid-body-formation-in-candida-tropicalis
#18
Irina Funk, Volker Sieber, Jochen Schmid
The unsaturated long-chain α,ω-dicarboxylic acid 1,18-cis-octadec-9-enedioic acid (cis-ODA) is a versatile precursor of various valuable compounds, such as polymers, and can be obtained from renewable resources. This makes cis-ODA highly attractive for the chemical industry where there is a growing interest in sustainable processes. However, chemical synthesis of the cis isomers is currently not feasible. In contrast, biotechnological production allows for highly specific and selective reactions. Therefore, we developed an efficient production strategy for cis-ODA using Candida tropicalis as a whole-cell biocatalyst for the biotransformation of oleic acid, which naturally occurs in various fats and oils...
October 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29059244/retraction-functional-characterization-of-two-structurally-novel-diacylglycerol-acyltransferase2-isozymes-responsible-for-the-enhanced-production-of-stearate-rich-storage-lipid-in-candida-tropicalis-sy005
#19
https://www.readbyqxmd.com/read/29054393/isolation-of-candida-spp-from-denture-related-stomatitis-in-par%C3%A3-brazil
#20
Lurdete Maria Rocha Gauch, Simone Soares Pedrosa, Fabíola Silveira-Gomes, Renata Antunes Esteves, Silvia Helena Marques-da-Silva
The aim of this study was to isolate and identify Candida species from the oral cavity of denture wearers with denture-related stomatitis who were attended at the University Federal of Pará (Belém City, Pará State, Brazil). A total of 36 denture wearers with denture-related stomatitis were included, and type I (50%), type II (33%) and type III (17%) stomatitis were observed. Candida spp. were isolated from 89% of the cases and included five different Candida species. C. albicans was the most frequently recovered species (78% of the cases), followed by C...
July 29, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
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