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Candida tropicalis

Ruifang Li, Ruiling Zhang, Yanhui Yang, Xueqin Wang, Yanjie Yi, Pei Fan, Zhengwei Liu, Chen Chen, Junpeng Chang
CGA-N12 is an antifungal peptide derived from human chromogranin A. In our previous investigation, CGA-N12 was found to have specific anti-candidal activity, though the mechanism of action remained unclear. Herein, we investigated the effects of CGA-N12 on mitochondria. We found that CGA-N12 induced an over-generation of intracellular reactive oxygen species and dissipation in mitochondrial membrane potential, in which the former plays key roles in the initiation of apoptosis and the latter is a sign of the cell apoptosis...
March 20, 2018: Biochemical Journal
Komathy Munusamy, Jamuna Vadivelu, Sun Tee Tay
BACKGROUND: Biofilm is known to contribute to the antifungal resistance of Candida yeasts. Aureobasidin A (AbA), a cyclic depsipeptide targeting fungal sphingolipid biosynthesis, has been shown to be effective against several Candida species. AIMS: The aim of this study was to investigate Candida biofilm growth morphology, its biomass, metabolic activity, and to determine the effects of AbA on the biofilm growth. METHODS: The biofilm forming ability of several clinical isolates of different Candida species from our culture collection was determined using established methods (crystal violet and XTT assays)...
March 12, 2018: Revista Iberoamericana de Micología
J Denis, M-P Ledoux, Y Nivoix, R Herbrecht
Triazoles compounds are first-line agents for the treatment of invasive fungal diseases. Isavuconazole is the most recent triazole compound, approved in 2015 by the FDA and the EMA to treat invasive aspergillosis and mucormycosis. We reviewed here the in vitro activity of isavuconazole against a vast spectrum of species. Isavuconazole MICs were evaluated using CLSI, EUCAST or Etest methods, with no significant differences between the technics. Low MIC50 and MIC90 (<1μg/mL) were described for isavuconazole against the majority of Candida spp...
March 10, 2018: Journal de Mycologie Médicale
S Salari, N Sadat Seddighi, P Ghasemi Nejad Almani
OBJECTIVE: Biofilm formation ability is one of the major virulence factors contributing to the pathogenesis of Candida species. Biofilms produced by Candida spp. cause complicated treatments and contribute to increasing unpleasant mortality rates. Nanoparticles of Fe3 O4 (Fe3 O4 -NPs) are considered due to their magnetic and biochemical properties, as well as their low costs. The purpose of present study was to determine biofilm formation ability in different Candida strains and evaluation of anti-biofilm effect of Fe3 O4 -NPs compared with FLC...
March 5, 2018: Journal de Mycologie Médicale
Omar Gómez-García, Dulce Andrade-Pavón, Elena Campos-Aldrete, Ricardo Ballinas-Indilí, Alfonso Méndez-Tenorio, Lourdes Villa-Tanaca, Cecilio Álvarez-Toledano
A series of 3-benzoyl imidazo[1,2- a ]pyrimidines, obtained from N -heteroarylformamidines in good yields, was tested in silico and in vitro for binding and inhibition of seven Candida species ( Candida albicans (ATCC 10231), Candida dubliniensis (CD36), Candida glabrata (CBS138), Candida guilliermondii (ATCC 6260), Candida kefyr , Candida krusei (ATCC 6358) and Candida tropicalis (MYA-3404)). To predict binding mode and energy, each compound was docked in the active site of the lanosterol 14α-demethylase enzyme (CYP51), essential for fungal growth of Candida species...
March 7, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Lais Lovison Sturaro, Tohru Gonoi, Ariane Fidelis Busso-Lopes, Cibele Aparecida Tararam, Carlos Emilio Levy, Luzia Lyra, Plinio Trabasso, Angélica Zaninelli Schreiber, Katsuhiko Kamei, Maria Luiza Moretti
A DNA microarray platform, based on the nucleotide sequences of the internal transcribed spacer regions (ITS1, ITS2) of the rRNA gene, was developed to identify 32 fungal pathogens at the species level. The probe sequences were spotted onto polycarbonate slides with a mini-microarray printer, and after the hybridization, the results were visible with the naked eye. The performance of the microarray platform was evaluated against the commercial automated systems (Vitek ® 2 and BD Phoenix™ systems) and DNA sequencing (gold standard)...
March 7, 2018: Journal of Clinical Microbiology
Maiken Cavling Arendrup, Joseph Meletiadis, Oscar Zaragoza, Karin Meinike Jørgensen, Laura Judith Marcos-Zambrano, Lamprini Kanioura, Manuel Cuenca-Estrella, Johan W Mouton, Jesus Guinea
OBJECTIVES: Rezafungin (CD101) is a new long-acting echinocandin allowing weekly dosing, currently undergoing phase-II clinical trials for invasive candidiasis. The aim of this study was to assess rezafungin's in vitro activity against the most frequent Candida species following the EUCAST methodology. METHODS: The susceptibility of 2,018 clinical Candida isolates was determined at four European laboratories. In parallel, six control strains were repeatedly tested...
March 2, 2018: Clinical Microbiology and Infection
Huan Trinh, Youngchul Yoo, Kyung-Hwa Won, Hien T T Ngo, Jung-Eun Yang, Jin-Gyeong Cho, Sang-Won Lee, Ki-Young Kim, Tae-Hoo Yi
PURPOSE: In traditional Korean medicine, Artemisia apiacea H. (ART) and Scutellaria baicalensis G. (SCU) are combined for the treatment of malaria and other malaria-like diseases. Because SCU is well-known as an antibacterial agent, the antimicrobial effect of a mixture of ART and SCU was investigated. METHODOLOGY: Plant samples were purchased from Kyungdong mart and extracted with 70 % ethanol. The in vitro antimicrobial activity of ART and SCU against pathogenic fungi (Aspergillus niger, Aspergillus oryzae, Candida albicans, Candida tropicalis and Candida glabrata), Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) was evaluated using a broth microdilution assay, modified-disc diffusion and agar dilution methods with further CH2Cl2-fractionated ART, SCU and a mixture of ART/SCU (at a ratio of 3 : 5) (THAN-1)...
March 5, 2018: Journal of Medical Microbiology
Chirom Aarti, Ameer Khusro, Rakesh Varghese, Mariadhas Valan Arasu, Paul Agastian, Naïf Abdullah Al-Dhabi, Soundharrajan Ilavenil, Ki Choon Choi
OBJECTIVE: To investigate the probiotic characteristics, anti-Candida activity, and antibiofilm attributes of Hentak derived Lactobacillus pentosus strain LAP1. DESIGN: The probiotic properties of strain LAP1 was depicted by adapting standard protocols. The anti-Candida and antibiofilm properties of isolate were determined using agar well diffusion assay and ELISA reader test, respectively. The time-kill assay was performed using viable colony count assay. Further, the co-aggregation property of strain LAP1 was determined based on standard methodology...
February 23, 2018: Archives of Oral Biology
Daniel Brustolin Ludwig, Luciana Erzinger Alves de Camargo, Najeh Maissar Khalil, Marcos Ereno Auler, Rubiana Mara Mainardes
Amphotericin B (AmB) is one of the most used drugs for the treatment of systemic fungal infections; however, the treatment causes several toxic manifestations, including nephrotoxicity and hemolytic anemia. Chitosan-coated poly(lactide-co-glycolide) (PLGA) nanoparticles containing AmB were developed with the aim to decrease AmB toxicity and propose the oral route for AmB delivery. In this work, the antifungal efficacy of chitosan-coated PLGA nanoparticles containing AmB was evaluated in 20 strains of fungus isolates from patients with vulvovaginal candidiasis (01 Candida glabrata and 03 Candida albicans), bloodstream infections (04 C...
March 1, 2018: Mycopathologia
Hiroshi Kakeya, Koichi Yamada, Yukihiro Kaneko, Katsunori Yanagihara, Kazuhiro Tateda, Shigefumi Maesaki, Yoshio Takesue, Kazunori Tomono, Jun-Ichi Kadota, Mitsuo Kaku, Yoshitsugu Miyazaki, Katsuhiko Kamei, Kazutoshi Shibuya, Yoshitiho Niki, Minoru Yoshida, Yoshihiro Sei
The Epidemiological Investigation Committee for Human Mycoses in Japan performed a retrospective epidemiological survey of candidemia and causative Candida species. Data from 2003 to 2014 were collected from 10 Japanese university hospitals. A total of 328,318 blood cultures were included. The prevalence of fungi in all cultures and in positive cultures were 0.58±0.09% and 4.46±0.66%, respectively. Among the results that were positive for Candida species (N=1,921), Candida albicans was the most common species (39...
2018: Medical Mycology Journal
Francisco Márquez, Isabel Iturrieta, Mario Calvo, Milton Urrutia, Patricio Godoy-Martínez
BACKGROUND: Candidemia disease in Chile is not very known, despite being a very common and serious disease. AIM: To analyze incidence and susceptibility profile of species from the genus Candida in Hospital Base Valdivia. MATERIAL AND METHODS: All candidemia cases presented between March 2009 and August 2011 in our institution were identified. Species distribution and susceptibility were studied through the disk diffusion method. RESULTS: Twenty-seven candidemia episodes were studied...
October 2017: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
Iris Nora Tiraboschi, Natalia Cintia Pozzi, Luciana Farías, Susana García, Norma Beatriz Fernández
BACKGROUND: The epidemiology of candidemia vary by region. AIM: To determine the epidemiology of candidemia in a hospital for 16 years. MATERIAL AND METHODS: Episodes of candidemia occurred in the Hospital de Clinicas of Buenos Aires were reviewed, from 01/01/98 to 31/12/13. RESULTS: 374 episodes of candidemia were identified. The incidence was 2.21/1,000 discharges and increased from 1.96 (1998-2005) to 2.25 (2006-2013) (p = 0...
October 2017: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
Mafalda Cavalheiro, Miguel Cacho Teixeira
Candida species are fungal pathogens known for their ability to cause superficial and systemic infections in the human host. These pathogens are able to persist inside the host due to the development of pathogenicity and multidrug resistance traits, often leading to the failure of therapeutic strategies. One specific feature of Candida species pathogenicity is their ability to form biofilms, which protects them from external factors such as host immune system defenses and antifungal drugs. This review focuses on the current threats and challenges when dealing with biofilms formed by Candida albicans, Candida glabrata, Candida tropicalis , and Candida parapsilosis , highlighting the differences between the four species...
2018: Frontiers in Medicine
Parisa Badiee, Maral Choopanizadeh, Abdolkarim Ghadimi Moghadam, Ali Hossaini Nasab, Hadis Jafarian, Ahmad Shamsizadeh, Jafar Soltani
Background and Objectives: Colonization of Candida species is common in pediatric patients admitted to hematology-oncology wards. The aim of this study was to identify colonized Candida species and their susceptibility patterns in hematologic pediatric patients. Materials and Methods: Samples were collected from mouth, nose, urine and stool of the patients admitted to five university hospitals and cultured on sabouraud dextrose agar. The isolates were identified by API 20 C AUX system and their susceptibility patterns were evaluated by CLSI M27-A3 and S4...
December 2017: Iranian Journal of Microbiology
Arthur J Morris, Karen Rogers, Wendy P McKinney, Sally A Roberts, Joshua T Freeman
OBJECTIVES: We have reviewed the susceptibility results of local yeast isolates performed 2001-2015 to record the impact of recently updated interpretive criteria, and epidemiological cutoff values (ECVs), for yeast species. METHODS: Testing was performed using Sensititre® YeastOne® and results interpreted following CLSI criteria or YeastOne derived ECVs. RESULTS: 2345 isolates were tested; 62% were from sterile body sites or tissue. Application of the new CLSI interpretative criteria for fluconazole increased the proportion of non-susceptible isolates of C...
February 24, 2018: Journal of Global Antimicrobial Resistance
Malgorzata Anna Dawgul, Daria Grzywacz, Beata Liberek, Wojciech Kamysz, Henryk Myszka
BACKGROUND: Candida albicans belongs to the most common fungal pathogens in humans, but recently an increased proliferation of strains called non-albicans Candida has been report-ed. Species belonging to this group are often characterised by a reduced susceptibility to anti-fungal agents. OBJECTIVE: In view of the emergence of non-albicans Candida and their resistance to available antifungals, an attempt has been made to develop novel effective agents. Biological activities of the N,N-dialkyl diosgenyl glycosides, which were previously synthesized, were determined...
February 26, 2018: Medicinal Chemistry
Francieli Gesleine Capote Bonato, Denis Vinicius Bonato, Ilan Munhoz Ayer, Larissa Fernandes Magalhães, Geórgia Modé Magalhães, Felipe Farias Pereira da Câmara Barros, Pedro Paulo Maia Teixeira, Leandro Zuccolotto Crivellenti, Melyssa Negri, Terezinha Inez Estivalet Svidzinski
To evaluate the pathophysiology of catheter-associated candiduria, the bladders of female mice were infected with Candida tropicalis. One group was implanted with a catheter fragment with preformed biofilm by cystotomy technique, while another group received, in separate, a sterile catheter fragment and a correspondent yeast suspension. The bladder tissues were examined by histopathology and the quantity of colony forming units was evaluated. All the animals presented inflammation and the presence of C. tropicalis was observed in the tissue within 72 h of the introduction of biofilm, while 75% of the mice remained infected after 144 h...
February 19, 2018: Microbial Pathogenesis
Gabriela C Alonso, Ana C Pavarina, Tábata V Sousa, Marlise I Klein
Biofilm production contributes to several human diseases, including oral candidiasis. Among the Candida species, Candida albicans is the most prevalent. The expression of virulence genes is implicated in the pathogenic potential of Candida biofilms. However, the evaluation of microbial gene expression from in vivo biofilm samples is not trivial, specifically, assessment via quantitative PCR (qPCR) can be a challenge because of several species present in clinical samples. Hence, the necessity of primers specificity...
February 14, 2018: Journal of Microbiological Methods
J N Vieira, A M Feijó, M E Bueno, C L Gonçalves, R G Lund, J F Mendes, J P V Villarreal, M M Villela, P S Nascente
The aim of this study was to evaluate the frequency of Candida species between a non-hospitalized and a hospitalized population. For this purpose, samples of saliva were sampled through sterile swabs, moistened in peptone water and rubbed in the oral cavity of 140 individuals, from which, 70 were hospitalized patients from the Medical Clinic of a Teaching Hospital and the other 70 were non-hospitalized subjects. All saliva samples were plated in Sabouraud Dextrose agar added with Chloramphenicol and incubated at 36 °C for 48 hours...
February 15, 2018: Brazilian Journal of Biology, Revista Brasleira de Biologia
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