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Samiksha Nayak, Archana Sridhara, Rita Melo, Luciana Richer, Natalie H Chee, Jiyoon Kim, Vincent Linder, David Steinmiller, Samuel K Sia, Maria Gomes-Solecki
Currently, diagnostic testing for Lyme disease is done by determination of the serologic responses to Borrelia burgdorferi antigens, with the exception of the early localized phase of disease where diagnosis must be done clinically. Here, we describe the use of microfluidics technology to develop a multiplexed rapid lab-on-a-chip point of care (POC) assay for the serologic diagnosis of human Lyme disease. Following ELISA screening of 12 candidate antigens, we tested 8 on a microfluidic diagnostic system, called mChip-Ld, using a set of 60 serological samples...
October 11, 2016: Scientific Reports
Xinyi Su, Queenie S W Tan, Bhav H Parikh, Alison Tan, Milan N Mehta, Yeo Sia Wey, Sai Bo Bo Tun, Ling-Jun Li, Xiao-Yan Han, Tien Y Wong, Walter Hunziker, Chi D Luu, Yuji Owada, Veluchamy A Barathi, Samuel S Zhang, Shyam S Chaurasia
PURPOSE: To characterize the mouse retina lacking fatty acid binding protein (FABP7-/-). METHODS: Immunohistochemistry (IHC) was performed in 8-week-old mice to localize FABP7 in the retina. Retinal thickness was measured using image-guided spectral-domain optical coherence topography images. Electroretinography was carried out to assess retinal function. Fundus photography and fundus fluorescein angiography were performed on FABP7-/- and littermate wild-type (WT) mice, and retinal vascular changes were calculated using Singapore I Vessel Assessment (SIVA) analysis...
June 1, 2016: Investigative Ophthalmology & Visual Science
Hasan E Abaci, Zongyou Guo, Abigail Coffman, Brian Gillette, Wen-Han Lee, Samuel K Sia, Angela M Christiano
Vascularization of engineered human skin constructs is crucial for recapitulation of systemic drug delivery and for their long-term survival, functionality, and viable engraftment. In this study, the latest microfabrication techniques are used and a novel bioengineering approach is established to micropattern spatially controlled and perfusable vascular networks in 3D human skin equivalents using both primary and induced pluripotent stem cell (iPSC)-derived endothelial cells. Using 3D printing technology makes it possible to control the geometry of the micropatterned vascular networks...
July 2016: Advanced Healthcare Materials
Kevin King, Luanda P Grazette, Dina N Paltoo, John T McDevitt, Samuel K Sia, Paddy M Barrett, Fred S Apple, Paul A Gurbel, Ralph Weissleder, Hilary Leeds, Erin J Iturriaga, Anupama Rao, Bishow Adhikari, Patrice Desvigne-Nickens, Zorina S Galis, Peter Libby
Point-of-care technologies (POC or POCT) are enabling innovative cardiovascular diagnostics that promise to improve patient care across diverse clinical settings. The National Heart, Lung, and Blood Institute convened a working group to discuss POCT in cardiovascular medicine. The multidisciplinary working group, which included clinicians, scientists, engineers, device manufacturers, regulatory officials, and program staff, reviewed the state of the POCT field; discussed opportunities for POCT to improve cardiovascular care, realize the promise of precision medicine, and advance the clinical research enterprise; and identified barriers facing translation and integration of POCT with existing clinical systems...
January 2016: JACC. Basic to Translational Science
Olga Ordeig, Sau Yin Chin, Sohyun Kim, Parag V Chitnis, Samuel K Sia
Implantable devices have a large potential to improve human health, but they are often made of biofouling materials that necessitate special coatings, rely on electrical connections for external communication, and require a continuous power source. This paper demonstrates an alternative platform, which we call iTAG (implantable thermally actuated gel), where an implanted capsule can be wirelessly controlled by ultrasound to trigger the release of compounds. We constructed a millimeter-sized capsule containing a co-polymer gel (NiPAAm-co-AAm) that contracts above body temperature (i...
2016: Scientific Reports
Samuel K Sia, Matthew P Owens
No abstract text is available yet for this article.
February 2016: Nature Biotechnology
Tiffany W Guo, Samiksha Nayak, Samuel K Sia
No abstract text is available yet for this article.
March 2016: Clinical Chemistry
Samuel K Sia, Matthew P Owens
No abstract text is available yet for this article.
December 9, 2015: Nature Biotechnology
Tiffany Guo, Ritish Patnaik, Kevin Kuhlmann, Alex J Rai, Samuel K Sia
It is traditionally difficult to incorporate two classes of diagnostic tests into a single platform. In this work, we demonstrate a microfluidic-based smartphone dongle that simultaneously measures concentration of hemoglobin and detects HIV antibodies. Specifically, we demonstrate how a previously published immunoassay device, which measured optical density of silver precipitation on gold colloids, can be expanded to quantitatively measure hemoglobin concentration via a colorimetric assay. By lysing whole blood components with CHAPS detergent, we achieved highly reproducible measurement of hemoglobin concentration with the device...
September 7, 2015: Lab on a Chip
Tassaneewan Laksanasopin, Tiffany W Guo, Samiksha Nayak, Archana A Sridhara, Shi Xie, Owolabi O Olowookere, Paolo Cadinu, Fanxing Meng, Natalie H Chee, Jiyoon Kim, Curtis D Chin, Elisaphane Munyazesa, Placidie Mugwaneza, Alex J Rai, Veronicah Mugisha, Arnold R Castro, David Steinmiller, Vincent Linder, Jessica E Justman, Sabin Nsanzimana, Samuel K Sia
This work demonstrates that a full laboratory-quality immunoassay can be run on a smartphone accessory. This low-cost dongle replicates all mechanical, optical, and electronic functions of a laboratory-based enzyme-linked immunosorbent assay (ELISA) without requiring any stored energy; all necessary power is drawn from a smartphone. Rwandan health care workers used the dongle to test whole blood obtained via fingerprick from 96 patients enrolling into care at prevention of mother-to-child transmission clinics or voluntary counseling and testing centers...
February 4, 2015: Science Translational Medicine
Tiffany W Guo, Tassaneewan Laksanasopin, Archana A Sridhara, Samiksha Nayak, Samuel K Sia
Here we describe a low-cost mobile device that combines cell-phone and satellite communication technologies with fluid miniaturization techniques for performing all essential functions of enzyme-linked immunosorbent assay (ELISA). Disease-specific antigens are immobilized on the microfluidic surface, and disease specific antibodies are captured on the surface and visualized with silver-gold amplification. The diagnostic result is automatically determined by the device by measuring the absorbance through the silver-gold amplification in the microchannel...
2015: Methods in Molecular Biology
M Flint Beal, David Oakes, Ira Shoulson, Claire Henchcliffe, Wendy R Galpern, Richard Haas, Jorge L Juncos, John G Nutt, Tiffini Smith Voss, Bernard Ravina, Clifford M Shults, Karen Helles, Victoria Snively, Mark F Lew, Brian Griebner, Arthur Watts, Shan Gao, Emmanuelle Pourcher, Louisette Bond, Katie Kompoliti, Pinky Agarwal, Cherissa Sia, Mandar Jog, Linda Cole, Munira Sultana, Roger Kurlan, Irene Richard, Cheryl Deeley, Cheryl H Waters, Angel Figueroa, Ani Arkun, Matthew Brodsky, William G Ondo, Christine B Hunter, Joohi Jimenez-Shahed, Alicia Palao, Janis M Miyasaki, Julie So, James Tetrud, Liza Reys, Katharine Smith, Carlos Singer, Anita Blenke, David S Russell, Candace Cotto, Joseph H Friedman, Margaret Lannon, Lin Zhang, Edward Drasby, Rajeev Kumar, Thyagarajan Subramanian, Donna Stuppy Ford, David A Grimes, Diane Cote, Jennifer Conway, Andrew D Siderowf, Marian Leslie Evatt, Barbara Sommerfeld, Abraham N Lieberman, Michael S Okun, Ramon L Rodriguez, Stacy Merritt, Camille Louise Swartz, W R Wayne Martin, Pamela King, Natividad Stover, Stephanie Guthrie, Ray L Watts, Anwar Ahmed, Hubert H Fernandez, Adrienna Winters, Zoltan Mari, Ted M Dawson, Becky Dunlop, Andrew S Feigin, Barbara Shannon, Melissa Jill Nirenberg, Mattson Ogg, Samuel A Ellias, Cathi-Ann Thomas, Karen Frei, Ivan Bodis-Wollner, Sofya Glazman, Thomas Mayer, Robert A Hauser, Rajesh Pahwa, April Langhammer, Ranjit Ranawaya, Lorelei Derwent, Kapil D Sethi, Buff Farrow, Rajan Prakash, Irene Litvan, Annette Robinson, Alok Sahay, Maureen Gartner, Vanessa K Hinson, Samuel Markind, Melisa Pelikan, Joel S Perlmutter, Johanna Hartlein, Eric Molho, Sharon Evans, Charles H Adler, Amy Duffy, Marlene Lind, Lawrence Elmer, Kathy Davis, Julia Spears, Stephanie Wilson, Maureen A Leehey, Neal Hermanowicz, Shari Niswonger, Holly A Shill, Sanja Obradov, Alex Rajput, Marilyn Cowper, Stephanie Lessig, David Song, Deborah Fontaine, Cindy Zadikoff, Karen Williams, Karen A Blindauer, Jo Bergholte, Clara Schindler Propsom, Mark A Stacy, Joanne Field, Dragos Mihaila, Mark Chilton, Ergun Y Uc, Jeri Sieren, David K Simon, Lauren Kraics, Althea Silver, James T Boyd, Robert W Hamill, Christopher Ingvoldstad, Jennifer Young, Karen Thomas, Sandra K Kostyk, Joanne Wojcieszek, Ronald F Pfeiffer, Michel Panisset, Monica Beland, Stephen G Reich, Michelle Cines, Nancy Zappala, Jean Rivest, Richard Zweig, L Pepper Lumina, Colette Lynn Hilliard, Stephen Grill, Marye Kellermann, Paul Tuite, Susan Rolandelli, Un Jung Kang, Joan Young, Jayaraman Rao, Maureen M Cook, Lawrence Severt, Karyn Boyar
IMPORTANCE: Coenzyme Q10 (CoQ10), an antioxidant that supports mitochondrial function, has been shown in preclinical Parkinson disease (PD) models to reduce the loss of dopamine neurons, and was safe and well tolerated in early-phase human studies. A previous phase II study suggested possible clinical benefit. OBJECTIVE: To examine whether CoQ10 could slow disease progression in early PD. DESIGN, SETTING, AND PARTICIPANTS: A phase III randomized, placebo-controlled, double-blind clinical trial at 67 North American sites consisting of participants 30 years of age or older who received a diagnosis of PD within 5 years and who had the following inclusion criteria: the presence of a rest tremor, bradykinesia, and rigidity; a modified Hoehn and Yahr stage of 2...
May 2014: JAMA Neurology
Zongyou Guo, Claire A Higgins, Brian M Gillette, Munenari Itoh, Noriko Umegaki, Karl Gledhill, Samuel K Sia, Angela M Christiano
The discovery of induced pluripotent stem cells (iPSCs) in 2006 was a major breakthrough for regenerative medicine. The establishment of patient-specific iPSCs has created the opportunity to model diseases in culture systems, with the potential to rapidly advance the drug discovery field. Current methods of drug discovery are inefficient, with a high proportion of drug candidates failing during clinical trials due to low efficacy and/or high toxicity. Many drugs fail toxicity testing during clinical trials, since the cells on which they have been tested do not adequately model three-dimensional tissues or their interaction with other organs in the body...
2013: Stem Cell Research & Therapy
Samuel K Sia
No abstract text is available yet for this article.
April 2014: Clinical Chemistry
George Eng, Benjamin W Lee, Hesam Parsa, Curtis D Chin, Jesse Schneider, Gary Linkov, Samuel K Sia, Gordana Vunjak-Novakovic
Cellular communities in living tissues act in concert to establish intricate microenvironments, with complexity difficult to recapitulate in vitro. We report a method for docking numerous cellularized hydrogel shapes (100-1,000 ┬Ám in size) into hydrogel templates to construct 3D cellular microenvironments. Each shape can be uniquely designed to contain customizable concentrations of cells and molecular species, and can be placed into any spatial configuration, providing extensive compositional and geometric tunability of shape-coded patterns using a highly biocompatible hydrogel material...
March 19, 2013: Proceedings of the National Academy of Sciences of the United States of America
Avital Mendelson, Yuk kee Cheung, Kamila Paluch, Mo Chen, Kimi Kong, Jiali Tan, Ziming Dong, Samuel K Sia, Jeremy J Mao
A multitude of cytotactic cues direct cell migration in development, cancer metastasis and wound healing. However, our understanding of cell motility remains fragmented partially because current migration devices only allow the study of independent factors. We developed a cell motility assay that allows competitive recruitment of a given cell population simultaneously by gradients of multiple cytotactic cues, observable under real-time imaging. Well-defined uniform gradients of cytotactic cues can be independently generated and sustained in each channel...
March 21, 2013: Lab on a Chip
Curtis D Chin, Yuk Kee Cheung, Tassaneewan Laksanasopin, Mario M Modena, Sau Yin Chin, Archana A Sridhara, David Steinmiller, Vincent Linder, Jules Mushingantahe, Gisele Umviligihozo, Etienne Karita, Lambert Mwambarangwe, Sarah L Braunstein, Janneke van de Wijgert, Ruben Sahabo, Jessica E Justman, Wafaa El-Sadr, Samuel K Sia
BACKGROUND: Collection of epidemiological data and care of patients are hampered by lack of access to laboratory diagnostic equipment and patients' health records in resource-limited settings. We engineered a low-cost mobile device that combines cell-phone and satellite communication technologies with fluid miniaturization techniques for performing all essential ELISA functions. METHODS: We assessed the device's ability to perform HIV serodiagnostic testing in Rwanda and synchronize results in real time with electronic health records...
April 2013: Clinical Chemistry
Curtis D Chin, Vincent Linder, Samuel K Sia
A large part of the excitement behind microfluidics is in its potential for producing practical devices, but surprisingly few lab-on-a-chip based technologies have been successfully introduced into the market. Here, we review current work in commercializing microfluidic technologies, with a focus on point-of-care diagnostics applications. We will also identify challenges to commercialization, including lessons drawn from our experience in Claros Diagnostics. Moving forward, we discuss the need to strike a balance between achieving real-world impact with integrated devices versus design of novel single microfluidic components...
June 21, 2012: Lab on a Chip
Samuel K Sia
No abstract text is available yet for this article.
November 2011: IEEE Pulse
Samuel K Sia, Curtis D Chin
Glucose meters allow rapid and quantitative measurement of blood sugar levels for diabetes sufferers worldwide. Now a new method allows this proven technology to be used to quantify a much wider range of analytes.
September 2011: Nature Chemistry
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