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https://www.readbyqxmd.com/read/28944507/peri-conceptional-under-nutrition-alters-transcriptomic-profile-in-the-endometrium-during-the-peri-implantation-period-the-study-in-domestic-pigs
#1
K Zglejc, M Martyniak, E Waszkiewicz, G Kotwica, A Franczak
Female under-nutrition during early pregnancy may affect the physiological pattern of the transcriptomic profile in the endometrium. We aimed to determine if restricted diet applied to females during peri-conceptional period, that is, from the onset of the oestrus until day nine of pregnancy, alters transcriptomic profile in the endometrium during the peri-implantation period. The restricted diet gilts were fed forage, in which the dose of proteins and energy had been reduced by 30% compared to normal diet...
September 24, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28943955/p53-inhibits-the-upregulation-of-sirtuin-1-expression-induced-by-c-myc
#2
Fang Yuan, Lu Liu, Yonghong Lei, Peifu Tang
Sirtuin 1 (Sirt1), a conserved NAD(+) dependent deacetylase, is a mediator of life span by calorie restriction. However, Sirt1 may paradoxically increase the risk of cancer. Accordingly, the expression level of Sirt1 is selectively elevated in numerous types of cancer cell; however, the mechanisms underlying the differential regulation remain largely unknown. The present study demonstrated that oncoprotein c-Myc was a direct regulator of Sirt1, which accounts for the upregulation of Sirt1 expression only in the cells without functional p53...
October 2017: Oncology Letters
https://www.readbyqxmd.com/read/28943405/detection-and-characterization-of-a-novel-rhabdovirus-in-aedes-cantans-mosquitoes-and-evidence-for-a-mosquito-associated-new-genus-in-the-family-rhabdoviridae
#3
Nariman Shahhosseini, Renke Lühken, Hanna Jöst, Stephanie Jansen, Jessica Börstler, Toni Rieger, Andreas Krüger, Anges Yadouleton, Renata de Mendonça Campos, Claudio Cesar Cirne-Santos, Davis Fernandes Ferreira, Rolf Garms, Norbert Becker, Egbert Tannich, Daniel Cadar, Jonas Schmidt-Chanasit
Thanks to recent advances in random amplification technologies, metagenomic surveillance expanded the number of novel, often unclassified viruses within the family Rhabdoviridae. Using a vector-enabled metagenomic (VEM) tool, we identified a novel rhabdovirus in Aedes cantans mosquitoes collected from Germany provisionally named Ohlsdorf virus (OHSDV). The OHSDV genome encodes the canonical rhabdovirus structural proteins (N, P, M, G and L) with alternative ORF in the P gene. Sequence analysis indicated that OHSDV exhibits a similar genome organization and characteristics compared to other mosquito-associated rhabdoviruses (Riverside virus, Tongilchon virus and North Creek virus)...
September 21, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28943344/polymorphisms-of-the-folate-metabolizing-enzymes-association-with-sle-susceptibility-and-in-silico-analysis
#4
Saeedeh Salimi, Farshid Keshavarzi, Abbas Mohammadpour-Gharehbagh, Mahdieh Moodi, Mahdieh Mousavi, Mohammad Karimian, Mahnaz Sandoughi
Systemic lupus erythematosus (SLE) is a typical autoimmune disorder with multiple organ involvement and unknown etiology. It has been shown that polymorphic variants of the genes encoding key enzymes of folate and methionine metabolism may influence DNA methylation. Genomic DNA was extracted from blood samples of 150 SLE patients and 160 controls, matching age, sex, and ethnicity. MTHFR rs1801133C>T and MTR rs1805087A>G polymorphisms were genotyped by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method...
September 21, 2017: Gene
https://www.readbyqxmd.com/read/28943103/lower-vertebrate-and-invertebrate-models-of-alzheimer-s-disease-a-review
#5
REVIEW
Neha Sharma, Navneet Khurana, Arunachalam Muthuraman
Alzheimer's disease is a common neurodegenerative disorder which is characterized by the presence of beta- amyloid protein and neurofibrillary tangles (NFTs) in the brain. Till now, various higher vertebrate models have been in use to study the pathophysiology of this disease. But, these models possess some limitations like ethical restrictions, high cost, difficult maintenance of large quantity and lesser reproducibility. Besides, various lower chordate animals like Danio rerio, Drosophila melanogaster, Caenorhabditis elegans and Ciona intestinalis have been proved to be an important model for the in vivo determination of targets of drugs with least limitations...
September 21, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28943084/horizontal-transfer-of-a-synthetic-metabolic-pathway-between-plant-species
#6
Yinghong Lu, Sandra Stegemann, Shreya Agrawal, Daniel Karcher, Stephanie Ruf, Ralph Bock
Transgene expression from the plastid (chloroplast) genome provides unique advantages, including high levels of foreign protein accumulation, convenient transgene stacking in operons, and increased biosafety due to exclusion of plastids from pollen transmission [1, 2]. However, applications in biotechnology and synthetic biology are severely restricted by the very small number of plant species whose plastid genomes currently can be transformed [3, 4]. Here we report a simple method for the introduction of useful plastid transgenes into non-transformable species...
September 16, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28942561/the-restriction-modification-system-of-bacillus-licheniformis-ms1-and-generation-of-a-readily-transformable-deletion-mutant
#7
Franziska Huff, Christine Muth, Christian Naumer, Friedhelm Meinhardt
Restriction modification systems (R-M systems), consisting of a restriction endonuclease and a cognate methyltransferase, constitute an effective means of a cell to protect itself from foreign DNA. Identification, characterization, and deletion of the restriction modification system BliMSI, a putative isoschizomer of ClaI from Caryophanon latum, were performed in the wild isolate Bacillus licheniformis MS1. BliMSI was produced as recombinant protein in Escherichia coli, purified, and in vitro analysis demonstrated identical restriction endonuclease activity as for ClaI...
September 23, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28942173/identification-and-characterization-of-cd4-t-cell-epitopes-on-manganese-transport-protein-c-of-staphylococcus-aureus
#8
Wei Yu, Lizi Wang, Mengyao Wang, Shuo Liu, Wanyu Li, Xintong Wang, Xiaoting Li, Simiao Yu, Di Yao, Jinzhu Ma, Liquan Yu, Jing Chen, Zhenyue Feng, Yudong Cui
Manganese transport protein C (MntC) of Staphylococcus aureus represents an excellent vaccine-candidate antigen. The important role of CD4(+) T cells in effective immunity against S. aureus infection was shown; however, CD4(+) T cell-specific epitopes on S. aureus MntC have not been well identified. Here, we used bioinformatics prediction algorithms to evaluate and identify nine candidate epitopes within MntC. Our results showed that peptide M8 emulsified in Freund's adjuvant induced a much higher cell-proliferation rate as compared with controls...
September 20, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28942102/skov3-cells-containing-a-truncated-arid1a-protein-have-a-restricted-genome-wide-response-to-glucocorticoids
#9
F E Stubbs, M T Birnie, S C Biddie, S L Lightman, B L Conway-Campbell
AT-rich interacting domain subunit 1a (ARID1a) is an essential SWI/SNF component frequently mutated in human cancers. ARID1a mutations have also been associated with glucocorticoid resistance, potentially related to the well-established role of the SWI/SNF complex in glucocorticoid target gene regulation. Glucocorticoids are steroid hormones important for regulating many physiological processes through the activation of the glucocorticoid receptor (GR). As GR interacts directly with ARID1a, we hypothesized that a truncating ARID mutation would interfere with GR-dependent gene regulation...
September 20, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28941614/development-of-a-simple-and-rapid-reverse-transcription-loop-mediated-isothermal-amplification-rt-lamp-assay-for-sensitive-detection-of-citrus-tristeza-virus
#10
Ashish Warghane, Pragati Misra, Sumit Bhose, Kajal Kumar Biswas, Ashwani Kumar Sharma, M Krishna Reddy, Dilip Kumar Ghosh
Tristeza is a devastating disease of citrus and reported to be present in almost all countries where it is cultivated as a commercial crop. The etiological agent of this disease is Citrus tristeza virus (CTV), a member of the genus Closterovirus with in the family Closteroviridae. The pathogen is restricted to the phloem tissue of the infected citrus plant and has a monopartite ss (+) RNA genome of ∼20kb size. Till date, there is no effective control measure available for this virus. Management of tristeza depends on destruction of CTV infected field plants, production of virus-free planting material for new orchard establishment and controlling viruliferous aphid vectors responsible for field spread of the pathogen...
September 20, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28941475/melanocytes-target-cells-of-an-hla-c-06-02-restricted-autoimmune-response-in-psoriasis
#11
REVIEW
Jörg Christoph Prinz
HLA-C*06:02 is the main psoriasis risk allele. By the unbiased analysis of a Vα3S1/Vβ13S1 T-cell receptor from pathogenic psoriatic CD8(+) T cells, we had recently proven that HLA-C*06:02 directs an autoimmune response against melanocytes through autoantigen presentation in psoriasis and identified ADAMTSL5 as a melanocyte autoantigen. We concluded that psoriasis is based on a melanocyte-specific immune response and that HLA-C*06:02 may predispose to psoriasis via this newly identified autoimmune pathway...
October 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28940557/skeletal-muscle-protein-accretion-rates-and-hindlimb-growth-are-reduced-in-late-gestation-intrauterine-growth-restricted-fetal-sheep
#12
Paul J Rozance, Laura Zastoupil, Stephanie R Wesolowski, David A Goldstrohm, Brittany Strahan, Melanie Cree-Green, Melinda Sheffield-Moore, Giacomo Meschia, William W Hay, Randall B Wilkening, Laura D Brown
Reduced skeletal muscle mass in the IUGR fetus persists into adulthood and may contribute to increased metabolic disease risk. To determine how placental insufficiency with reduced oxygen and nutrient supply to the fetus affects hindlimb blood flow, substrate uptake, and protein accretion rates in skeletal muscle, late gestation CON (n = 8) and IUGR (n = 13) fetal sheep were catheterized with aortic and femoral catheters and a flow transducer around the external iliac artery. Muscle protein kinetic rates were measured using isotopic tracers...
September 22, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28940253/pbrm1-restricts-the-basal-activity-of-innate-immune-system-through-repressing-rig-i-like-receptor-signaling-and-is-a-potential-prognostic-biomarker-for-colon-cancer
#13
Xing-Sheng Shu, Yingying Zhao, Yanmei Sun, Lan Zhong, Yingduan Cheng, Yixiang Zhang, Kaile Ning, Qian Tao, Yejun Wang, Ying Ying
It has long been known that patients suffering from inflammatory bowel disease (IBD) have an increased risk of developing colorectal cancer (CRC). The innate immune system of host cells provides a first-line defence against pathogenic infection, whereas uncontrolled inflammatory response under homeostatic conditions usually leads to pathological consequences, as exemplified by the chronic inflammation of IBD. The key molecules and pathways keeping innate immunity in check are still poorly defined. Here, we report that chromatin remodeler polybromo-1 (PBRM1) is a repressor of innate immune signaling mediated by RIG-I-like receptors (RLRs)...
September 20, 2017: Journal of Pathology
https://www.readbyqxmd.com/read/28939596/the-f-box-protein-zygo1-mediates-bouquet-formation-to-promote-homologous-pairing-synapsis-and-recombination-in-rice-meiosis
#14
Fanfan Zhang, Ding Tang, Yi Shen, Zhihui Xue, Wenqing Shi, Lijun Ren, Guijie Du, Yafei Li, Zhukuan Cheng
Telomere bouquet formation, a highly conserved meiotic event, plays an important role in homologous pairing and therefore progression of meiosis; however, the underlying molecular mechanism remains largely unknown. Here, we identified ZYGOTENE 1 (ZYGO1), a novel F-box protein in rice (Oryza sativa), and verified its essential role in bouquet formation during early meiosis. In zygo1 mutants, zygotene chromosome aggregation and telomere clustering failed to occur. The suppressed telomere clustering in homologous pairing aberration in rice meiosis 1 (pair1) zygo1 and rice completion of meiotic recombination (Oscom1) zygo1 double mutants, together with the altered localization of OsSAD1 (a SUN protein associated with the nuclear envelope) in zygo1 showed that ZYGO1 has a significant function in bouquet formation...
September 22, 2017: Plant Cell
https://www.readbyqxmd.com/read/28939594/rnase-h1-cooperates-with-dna-gyrases-to-restrict-r-loops-and-maintain-genome-integrity-in-arabidopsis-chloroplasts
#15
Zhuo Yang, Quancan Hou, Lingling Cheng, Wei Xu, Yantao Hong, Shuai Li, Qianwen Sun
Maintaining organellar genome integrity is essential for eukaryotic cells, and many factors can threaten genome integrity. R-loops are DNA:RNA duplexes produced during transcription, with the non-templated DNA forming a single-stranded region. R-loops function in the regulation of transcription, DNA replication, and DNA repair, but can also be susceptible to lesions that form double-stranded breaks and thus induce genome instability. From investigating the function of a plant chloroplast localized R-loop removing enzyme AtRNH1C, we have found that it is responsible for plastid R-loop homeostasis, chloroplast genome instability and development...
September 22, 2017: Plant Cell
https://www.readbyqxmd.com/read/28938793/effects-of-protein-or-amino-acid-supplementation-on-the-physical-growth-of-young-children-in-low-income-countries
#16
Joanne E Arsenault, Kenneth H Brown
Child growth stunting is common in low-income countries, possibly due to insufficient protein intakes. Most previous studies have concluded that children's protein intakes are adequate in relation to estimated requirements, but these studies did not consider issues of protein digestibility and effects of infection on dietary protein utilization. Using an alternative approach to assess the possible role of protein inadequacy in children's growth restriction, the results of 18 intervention trials in which supplementary protein or amino acids were provided to children ages 6-35 months and growth outcomes were reviewed...
September 1, 2017: Nutrition Reviews
https://www.readbyqxmd.com/read/28938603/inhibition-of-avian-tumor-virus-replication-by-ccch-type-zinc-finger-antiviral-protein
#17
Mingjun Zhu, Xiaoqian Ma, Xiyao Cui, Jing Zhou, Chengui Li, Libo Huang, Yingli Shang, Ziqiang Cheng
CCCH type zinc finger antiviral protein (ZAP) is a host restriction factor that inhibits the replication of a variety of viruses in mammals. However, little is known about its antiviral activity on avian tumor virus. Avian leukosis virus subgroup J (ALV-J), an oncogenic retrovirus, induces myelocytomas and various other tumors in meat and egg type chickens. Here, we identified a chicken ZAP (chZAP) that increased at early stage, and subsequently decreased after infection of ALV-J in DF-1 cells, indicating the inducible feature of the endogenous chZAP...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938470/genetic-disruption-of-21-hydroxylase-in-zebrafish-causes-interrenal-hyperplasia
#18
Helen Eachus, Andreas Zaucker, James A Oakes, Aliesha Griffin, Meltem Weger, Tülay Güran, Angela Taylor, Abigail Harris, Andy Greenfield, Jonathan L Quanson, Karl-Heinz Storbeck, Vincent T Cunliffe, Ferenc Müller, Nils Krone
Congenital adrenal hyperplasia is a group of common inherited disorders leading to glucocorticoid deficiency. Most cases are caused by 21-hydroxylase deficiency (21OHD). The systemic consequences of imbalanced steroid hormone biosynthesis due to severe 21OHD remains poorly understood. Therefore, we have developed a zebrafish model for 21OHD, which focuses on the impairment of glucocorticoid biosynthesis. A single 21-hydroxylase gene (cyp21a2) is annotated in the zebrafish genome based on sequence homology. Our in silico analysis of the Cyp21a2 protein sequence suggests a sufficient degree of similarity for the usage of zebrafish cyp21a2 to model aspects of human 21OHD in vivo...
September 13, 2017: Endocrinology
https://www.readbyqxmd.com/read/28938067/a-single-chain-photoswitchable-crispr-cas9-architecture-for-light-inducible-gene-editing-and-transcription
#19
Xin X Zhou, Xinzhi Zou, Hokyung K Chung, Yuchen Gao, Yanxia Liu, Lei S Qi, Michael Z Lin
Optical control of CRISPR-Cas9-derived proteins would be useful for restricting gene editing or transcriptional regulation to desired times and places. Optical control of Cas9 functions has been achieved with photouncageable unnatural amino acids or by using light-induced protein interactions to reconstitute Cas9-mediated functions from two polypeptides. However, these methods have only been applied to one Cas9 species and have not been used for optical control of different perturbations at two genes. Here, we use photodissociable dimeric fluorescent protein domains to engineer single-chain photoswitchable Cas9 (ps-Cas9) proteins in which the DNA-binding cleft is occluded at baseline and opened upon illumination...
September 22, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28936222/the-soybean-rfg1-gene-restricts-nodulation-by-sinorhizobium-fredii-usda193
#20
Yinglun Fan, Jinge Liu, Shanhua Lyu, Qi Wang, Shengming Yang, Hongyan Zhu
Sinorhizobium fredii is a fast-growing rhizobial species that can establish a nitrogen-fixing symbiosis with a wide range of legume species including soybeans (Glycine max). In soybeans, this interaction shows a high level of specificity such that particular S. fredii strains nodulate only a limited set of plant genotypes. Here we report the identification of a dominant gene in soybeans that restricts nodulation with S. fredii USDA193. Genetic mapping in an F2 population revealed co-segregation of the underlying locus with the previously cloned Rfg1 gene...
2017: Frontiers in Plant Science
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