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https://www.readbyqxmd.com/read/28092365/cancer-cell-chemokines-direct-chemotaxis-of-activated-stellate-cells-in-pancreatic-ductal-adenocarcinoma
#1
Ishan Roy, Kathleen A Boyle, Emily P Vonderhaar, Noah P Zimmerman, Egal Gorse, A Craig Mackinnon, Rosa F Hwang, Janusz Franco-Barraza, Edna Cukierman, Susan Tsai, Douglas B Evans, Michael B Dwinell
The mechanisms by which the extreme desmoplasia observed in pancreatic tumors develops remain unknown and its role in pancreatic cancer progression is unsettled. Chemokines have a key role in the recruitment of a wide variety of cell types in health and disease. Transcript and protein profile analyses of human and murine cell lines and human tissue specimens revealed a consistent elevation in the receptors CCR10 and CXCR6, as well as their respective ligands CCL28 and CXCL16. Elevated ligand expression was restricted to tumor cells, whereas receptors were in both epithelial and stromal cells...
January 16, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/28090761/modest-overexpression-of-foxo-maintains-cardiac-proteostasis-and-ameliorates-age-associated-functional-decline
#2
Anna C Blice-Baum, Alexander C Zambon, Gaurav Kaushik, Meera C Viswanathan, Adam J Engler, Rolf Bodmer, Anthony Cammarato
Heart performance declines with age. Impaired protein quality control (PQC), due to reduced ubiquitin-proteasome system (UPS) activity, autophagic function, and/or chaperone-mediated protein refolding, contributes to cardiac deterioration. The transcription factor FOXO participates in regulating genes involved in PQC, senescence, and numerous other processes. Here, a comprehensive approach, involving molecular genetics, novel assays to probe insect cardiac physiology, and bioinformatics, was utilized to investigate the influence of heart-restricted manipulation of dFOXO expression in the rapidly aging Drosophila melanogaster model...
February 2017: Aging Cell
https://www.readbyqxmd.com/read/28090561/diversity-in-rotavirus-host-glycan-interactions-a-sweet-spectrum
#3
REVIEW
Sasirekha Ramani, Liya Hu, B V Venkataram Prasad, Mary K Estes
Interaction with cellular glycans is a critical initial step in the pathogenesis of many infectious agents. Technological advances in glycobiology have expanded the repertoire of studies delineating host glycan-pathogen interactions. For rotavirus, the VP8* domain of the outer capsid spike protein VP4 is known to interact with cellular glycans. Sialic acid was considered the key cellular attachment factor for rotaviruses for decades. Although this is true for many rotavirus strains causing infections in animals, glycan array screens show that many human rotavirus strains bind nonsialylated glycoconjugates, called histo-blood group antigens, in a strain-specific manner...
May 2016: Cellular and Molecular Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28090279/association-of-atp-binding-cassette-transporter-a1-abca1-565-c-t-gene-polymorphism-with-hypoalphalipoproteinemia-and-serum-lipids-il-6-and-crp-levels
#4
Mohammad Mahdi Babashamsi, Sohrab Halalkhor, Hamid Moradi Firouzjah, Hadi Parsian, Seyed Farzad Jalali, Mohammad Babashamsi
BACKGROUND: ATP-binding cassette transporter A1 (ABCA1) is a membrane integral protein which plays a vital role in High Density Lipoprotein (HDL) metabolism and exerts a protective effect against Hypoalphalipoproteinemia (HA) by mediation of rate-limiting step in HDL biogenesis. In addition, this protein possesses anti-inflammatory effects by inhibiting the production of some inflammatory cytokines in macrophages. This study investigated the association of ABCA1-565 C/T gene polymorphism with HA and serum lipids, IL-6 and CRP levels...
January 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28089933/hfb7-a-novel-orphan-hydrophobin-of-the-harzianum-and-virens-clades-of-trichoderma-is-involved-in-response-to-biotic-and-abiotic-stresses
#5
Agnes Przylucka, Gunseli Bayram Akcapinar, Komal Chenthamara, Feng Cai, Marica Grujuc, Juriy Karpenko, Miriam Livoi, Qirong Shen, Christian P Kubicek, Irina S Druzhinina
Hydrophobins are small secreted cysteine-rich proteins exclusively found in fungi. They are able to self-assemble in single molecular layers at hydrophobic-hydrophilic interfaces and can therefore be directly involved in establishment of fungi in their habitat. The genomes of filamentous mycotrophic fungi Trichoderma encode a rich diversity of hydrophobins, which are divided in several groups based on their structure and evolution. Here we describe a new member of class II hydrophobins, HFB7, that has a taxonomically restricted occurrence in Harzianum and Virens clades of Trichoderma...
January 12, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/28089775/degenerative-effects-of-cobalt-chloride-treatment-on-neurons-and-microglia-in-a-porcine-retina-organ-culture-model
#6
S Kuehn, J Hurst, F Rensinghoff, T Tsai, S Grauthoff, Y Satgunarajah, H B Dick, S Schnichels, S C Joachim
In order to understand the pathological processes of retinal diseases, experimental models are necessary. Cobalt, as part of the vitamin B12 complex, is important for neuronal integrity. However, it is known that high quantities of cobalt induce cytotoxic mechanisms via hypoxia mimicry. Therefore, we tested the degenerative effect of cobalt chloride (CoCl2) on neurons and microglia in a porcine retina organ culture model. Organotypic cultures of porcine retinas were cultured and treated with different concentrations of CoCl2 (0, 100, 300 and 500 μM) for 48 h...
January 12, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28089773/mitochondrial-diseases-yeast-as-a-model-for-the-study-of-suppressors
#7
Silvia Francisci, Arianna Montanari
Mitochondrial (mt) tRNA gene mutations are an important cause of human morbidity and are associated with different syndromes. We have previously shown that the mitochondrial protein synthesis elongation factor EF-Tu and isolated sequences from the carboxy-terminal domain of yeast and human mt leucyl-tRNA synthetases (LeuRS), have a wide range of suppression capability among different yeast mt tRNA mutants having defective respiratory phenotype. Here we show that the rescuing capability can be restricted to a specific sequence of six amino acids from the carboxy-terminal domain of mt LeuRS...
January 12, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28089629/a-rack1-like-protein-regulates-hyphal-morphogenesis-root-entry-and-in-vivo-virulence-in-verticillium-dahlia
#8
Lei Yuan, Yaxin Su, Shuai Zhou, Yigao Feng, Wangzhen Guo, Xinyu Wang
To identify key genes expressed in Verticillium dahliae in early stages of infection of cotton roots, spore suspensions of eight V. dahliae isolates with different virulence levels were induced by cotton roots and genes expressed in these isolates during the early stages of infection were profiled. A gene that was differentially expressed between highly and less virulent strains was identified. Cloning and bioinformatics analysis of the gene suggested that it belongs to the putative Gβ-like/RACK1 protein family, and has seven WD40 domains...
January 9, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/28089080/salba-chia-salvia-hispanica-l-in-the-treatment-of-overweight-and-obese-patients-with-type-2-diabetes-a-double-blind-randomized-controlled-trial
#9
V Vuksan, A L Jenkins, C Brissette, L Choleva, E Jovanovski, A L Gibbs, R P Bazinet, F Au-Yeung, A Zurbau, H V T Ho, L Duvnjak, J L Sievenpiper, R G Josse, A Hanna
BACKGROUND AND AIM: Preliminary findings indicate that consumption of Salba-chia (Salvia hispanica L.), an ancient seed, improves management of type 2 diabetes and suppresses appetite. The aim of this study was to assesse the effect of Salba-chia on body weight, visceral obesity and obesity-related risk factors in overweight and obese adults with type 2 diabetes. METHODS: A double-blind, randomized, controlled trial with two parallel groups involved 77 overweight or obese patients with type 2 diabetes (HbA1c: 6...
December 9, 2016: Nutrition, Metabolism, and Cardiovascular Diseases: NMCD
https://www.readbyqxmd.com/read/28088654/transcriptional-response-of-skeletal-muscle-to-a-low-protein-perinatal-diet-in-rat-offspring-at-different-ages-the-role-of-key-enzymes-of-glucose-fatty-acid-oxidation
#10
José Luiz de Brito Alves, Ana Elisa Toscano, João Henrique da Costa-Silva, Hubert Vidal, Carol Góis Leandro, Luciano Pirola
Skeletal muscle is a plastic tissue during development with distinctive acute and chronic response to maternal protein restriction. This study evaluated gene and protein expression of key-enzymes of glycolytic pathway (HK2, PFK, PDK4 and CS), and fatty acid oxidation (CPT1 and β-HAD) of two different types of skeletal muscle [soleus and extensor digitorium longus (EDL)] from offspring rats at 30 and 90 days of age, exposed to maternal isoenergetic low protein diet throughout gestation and lactation. Pups from dams fed 17% protein diet (n=5, normal protein, Np), and low protein pups from dams fed 8% casein diet (low protein, Lp, n=5) were evaluated...
December 15, 2016: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28087701/the-ubiquitin-ligase-nedd4l-regulates-the-na-k-2cl-co-transporter-nkcc1-slc12a2-in-the-colon
#11
Chong Jiang, Hiroshi Kawabe, Daniela Rotin
The ubiquitin ligase Nedd4-like (Nedd4L, or Nedd4-2) binds to and regulates stability of the epithelial Na+ channel (ENaC) in salt absorbing epithelia in the kidney, lung, and other tissues. Its role in the distal colon, which also absorbs salt and fluid and expresses ENaC, is unknown. Using conditional knockout approach to knockout Nedd4L in mice intestinal epithelium (Nedd4Lf/f;Vil-CreERT2) we show here that Nedd4L depletion leads to a higher steady-state short circuit current (Isc) in mouse distal colon tissue relative to controls...
January 13, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28087699/activation-of-c-abl-kinase-potentiates-the-anti-myeloma-drug-lenalidomide-by-promoting-dda1-recruitment-to-the-crl4-ubiquitin-ligase
#12
Shaobing Gao, Chenlu Geng, Tianyu Song, Xuanru Lin, Jiye Liu, Zhen Cai, Yong Cang
Cullin Ring Ligase 4 (CRL4), a complex of Cul4 and DDB1, regulates cell cycle, DNA damage repair, and chromatin replication by targeting a variety of substrates for ubiquitination. CRL4 is also hijacked by viral proteins or thalidomide-derived compounds to degrade host restriction factors. Here we report that the c-Abl non-receptor kinase phosphorylates DDB1 at residue Y316 to recruit a small regulatory protein DDA1, leading to increased substrate ubiquitination. Pharmacological inhibition or genetic ablation of the Abl-DDB1-DDA1 axis decreases the ubiquitination of CRL4 substrates, including IKZF1 and IKZF3 in lenalidomide-treated multiple myeloma cells...
January 13, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28087685/bst-2-restricts-iav-release-and-is-countered-by-the-viral-m2-protein
#13
Siqi Hu, Lijuan Yin, Shan Mei, Jian Li, Fengwen Xu, Hong Sun, Xiaoman Liu, Shan Cen, Chen Liang, Ailing Li, Fei Guo
BST-2 (tetherin, CD317, HM1.24) is induced by interferon and restricts virus release by tethering the enveloped viruses to the cell surface. The effect of BST-2 on influenza A virus (IAV) infection has been inconclusive. In this study, we report that BST-2 diminishes the production of IAV virus-like particles, that are generated by viral NA and HA proteins, to a much greater degree than inhibits the production of wild type IAV particles. This relatively weaker inhibition of IAV is associated with reduction of BST-2 levels, which is caused by the M2 protein that interacts with BST-2 and leads to downregulation of cell surface BST-2 via the proteasomal pathway...
January 13, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28087520/atheroprotective-vaccination-with-mhc-ii-restricted-apob-peptides-induces-peritoneal-il-10-producing-cd4-t-cells
#14
Takayuki Kimura, Kevin Tse, Sara McArdle, Teresa Gerhardt, Jacqueline Miller, Zbigniew Mikulski, John Sidney, Alessandro Sette, Dennis Wolf, Klaus Ley
Although immunization with MHC class II-restricted ApoB peptides has been shown to be atheroprotective, the mechanism is unclear. Here, we investigated CD4(+) T cell populations in immunized atherosclerotic mice. Peptides (16-mers) from mouse ApoB, the core protein of low-density lipoprotein (LDL), were screened for binding to I-A(b) by computer prediction and confirmed by radiolabeled peptide competition. Three new peptides, P101 (FGKQGFFPDSVNKALY, 5.5 nM IC50), P102 (TLYALSHAVNSYFDVD, 6.8 nM), and P103 (LYYKEDKTSLSASAAS, 95 nM) were tested in an atherosclerosis model (Apoe(-/-) mice on western diet)...
January 13, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28081290/membrane-microdomains-and-the-cytoskeleton-constrain-athir1-dynamics-and-facilitate-the-formation-of-an-athir1-associated-immune-complex
#15
Xueqin Lv, Yanping Jing, Jianwei Xiao, Yongdeng Zhang, Yingfang Zhu, Russell Julian, Jinxing Lin
Arabidopsis hypersensitive induced reaction (AtHIR) proteins function in plant innate immunity. However, the underlying mechanisms by which AtHIRs participate in plant immunity remain elusive. Here, using VA-TIRFM and FLIM-FRET, we revealed that AtHIR1 is present in membrane microdomains and co-localizes with the membrane microdomain marker REM1.3. Single-particle tracking analysis revealed that membrane microdomains and the cytoskeleton, especially microtubules, restrict the lateral mobility of AtHIR1 at the plasma membrane and facilitate its oligomerization...
January 12, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28081275/role-of-xrcc1-gene-polymorphisms-in-non-small-cell-lung-cancer-cisplatin-based-chemotherapy-and-their-effect-on-clinical-and-pathological-characteristics
#16
H F Liu, J S Liu, J H Deng, R R Wu
Non-small cell lung cancer (NSCLC) is the most common cancer globally. The XRCC1 protein interacts with ligase and poly(ADP-ribose) polymerase to repair cisplatin-induced DNA damage. The authors of previous studies have reported XRCC1 Arg399Gln, Arg280His, and Arg194Trp polymorphisms and advanced NSCLC prognosis, but the results are inconclusive. We investigated the association between clinical outcome and XRCC1 Arg399Gln, Arg280His, and Arg194Trp polymorphisms in advanced NSCLC patients treated with cisplatin...
December 23, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28081174/definition-of-human-epitopes-recognized-in-tetanus-toxoid-and-development-of-an-assay-strategy-to-detect-ex-vivo-tetanus-cd4-t-cell-responses
#17
Ricardo da Silva Antunes, Sinu Paul, John Sidney, Daniela Weiskopf, Jennifer M Dan, Elizabeth Phillips, Simon Mallal, Shane Crotty, Alessandro Sette, Cecilia S Lindestam Arlehamn
Despite widespread uses of tetanus toxoid (TT) as a vaccine, model antigen and protein carrier, TT epitopes have been poorly characterized. Herein we defined the human CD4+ T cell epitope repertoire by reevaluation of previously described epitopes and evaluation of those derived from prediction of HLA Class II binding. Forty-seven epitopes were identified following in vitro TT stimulation, with 28 epitopes accounting for 90% of the total response. Despite this diverse range of epitopes, individual responses were associated with only a few immunodominant epitopes, with each donor responding on average to 3 epitopes...
2017: PloS One
https://www.readbyqxmd.com/read/28079155/dietary-phytochemical-peitc-restricts-tumor-development-via-modulation-of-epigenetic-writers-and-erasers
#18
Jung Eun Park, Yang Sun, Sai Kiang Lim, James P Tam, Matthijs Dekker, Hong Chen, Siu Kwan Sze
Dietary intake of bioactive phytochemicals including the cruciferous vegetable derivative phenethyl isothiocyanate (PEITC) can reduce risk of human cancers, but possible epigenetic mechanisms of these effects are yet unknown. We therefore sought to identify the molecular basis of PEITC-mediated epigenetic tumor restriction. Colon cancer cells treated with low-dose PEITC for >1 month exhibited stable alterations in expression profile of epigenetic writers/erasers and chromatin-binding of histone deacetylases (HDACs) and Polycomb-group (PcG) proteins...
January 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28078708/blood-vessel-formation-during-tail-regeneration-in-the-leopard-gecko-eublepharis-macularius-the-blastema-is-not-avascular
#19
Samantha L Payne, Hanna M Peacock, Matthew K Vickaryous
Unique among amniotes, many lizards are able to self-detach (autotomize) their tail and then regenerate a replacement. Tail regeneration involves the formation of a blastema, an accumulation of proliferating cells at the site of autotomy. Over time, cells of the blastema give rise to most of the tissues in the replacement tail. In non-amniotes capable of regenerating (such as urodeles and some teleost fish), the blastema is reported to be essentially avascular until tissue differentiation takes place. For tail regenerating lizards less is known...
January 12, 2017: Journal of Morphology
https://www.readbyqxmd.com/read/28077806/low-protein-diet-for-the-prevention-of-renal-failure
#20
Shaw Watanabe
The prevalence of chronic kidney disease (CKD) is estimated to be 8-16% worldwide, and it is increasing. CKD is a risk factor for heart attack and stroke, and it can progress to kidney failure requiring dialysis or transplantation. Recently, diabetic nephropathy has become the most common cause of CKD. In Japan, the cumulative probability of requiring hemodialysis by the age 80 years is 1/50 in males and 1/100 in females. The number of patients under hemodialysis in Japan exceeded 320,000 in 2014, among which 38,000 were newcomers and 27,000 died...
2017: Proceedings of the Japan Academy. Series B, Physical and Biological Sciences
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