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https://www.readbyqxmd.com/read/27915164/can-the-cerebral-regional-oxygen-saturation-be-a-perfusion-parameter-in-shock
#1
Ashraf Al Tayar, Amr Abouelela, Khaja Mohiuddeen
INTRODUCTION: Shock, defined as a state of tissue hypoperfusion and the tissues reperfusion, is the main goal of management of shock. Increase in central venous saturation (CvSo2) and decrease in blood lactate level are useful in assessment of adequacy of tissue perfusion. Near-infrared spectroscopy is a noninvasive way to observe real-time changes in regional cerebral saturation and has been used in patients with different brain diseases. There is a small body of literature suggesting that cerebral regional oxygen saturation (CrSo2) monitoring added a value in assessment and management of intensive care unit (ICU) patients...
November 10, 2016: Journal of Critical Care
https://www.readbyqxmd.com/read/27695476/srbreak-a-read-depth-and-split-read-framework-to-identify-breakpoints-of-different-events-inside-simple-copy-number-variable-regions
#2
Hoang T Nguyen, James Boocock, Tony R Merriman, Michael A Black
Copy-number variation (CNV) has been associated with increased risk of complex diseases. High-throughput sequencing (HTS) technologies facilitate the detection of copy-number variable regions (CNVRs) and their breakpoints. This helps in understanding genome structure as well as their evolution process. Various approaches have been proposed for detecting CNV breakpoints, but currently it is still challenging for tools based on a single analysis method to identify breakpoints of CNVs. It has been shown, however, that pipelines which integrate multiple approaches are able to report more reliable breakpoints...
2016: Frontiers in Genetics
https://www.readbyqxmd.com/read/27514781/identification-and-characterization-of-a-novel-toll-like-receptor-4-homologue-in-blunt-snout-bream-megalobrama-amblycephala
#3
Leilei Tang, Xinying Xiang, Yuhong Jiang, Yina Lv, Yi Zhou, Huan Zhong, Jun Xiao, Fuyun Zhang, Hongyang Jiang, Jinpeng Yan
Toll-like receptors (TLRs) are central players in the innate immune system in response to a wide range of pathogen infection. Among various TLRs, TLR4 plays a key role in recognition of bacterial lipopolysaccharides (LPS). In the present study, we identified and characterized a novel TLR4 homologue (maTLR4b) in blunt snout bream (Megalobrama amblycephala) which was significantly distinct from previously reported M. amblycephala TLR4 (tentatively named maTLR4a). The results showed that the complete cDNA sequence of maTLR4b was 3261 bp with an open reading frame encoding a polypeptide of 820 amino acids, and that its genomic sequence was 3793 bp, which had 3 exons...
October 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27495190/blood-pressure-cuff-comparability-study
#4
Yechiam Ostchega, Tatiana Nwankwo, Guangyu Zhang, Michele Chiappa
BACKGROUND: Manufacturer-supplied blood pressure (BP) cuffs are part of the automatic oscillometric BP devices algorithm. MATERIALS AND METHODS: This study assessed the differences in BP values using the Omron HEM 907-XL (Omron) device with two types of cuffs: the Baum cuff (BC) and the supplied Omron cuff (OC). A sample of 102 adults participated in the study, 34 per cuff size (adult, large, and extra-large). After a 5-min resting period, three pairs of BP determinations (systolic and diastolic) were taken simultaneously on both arms...
August 4, 2016: Blood Pressure Monitoring
https://www.readbyqxmd.com/read/27386274/response-of-a-mu-class-glutathione-s-transferase-from-black-tiger-shrimp-penaeus-monodon-to-aflatoxin-b1-exposure
#5
Yun Wang, Lihui Liu, Jianhua Huang, Yafei Duan, Jun Wang, Mingjun Fu, Heizhao Lin
Glutathione S-transferases (GSTs) are a family of multifunctional phase II enzymes that are involved in the detoxification of exogenous and endogenous compounds. In this study, a full-length cDNA of Mu-class GST (PmMuGST) was isolated from the hepatopancreas of Penaeus monodon using rapid amplification of cDNA ends method. The full length cDNA of PmMuGST is 867 bp, contains an open read frame of 660 bp, and encodes a polypeptide of 219 amino acids with a molecular mass of 25.61 kDa and pI of 6.15. Sequence analysis indicated that the predicted protein sequence of PmMuGST was very similar to (86 %) that of Litopenaeus vannamei...
2016: SpringerPlus
https://www.readbyqxmd.com/read/26272638/identification-of-a-novel-clip-domain-serine-proteinase-sp-csp-and-its-roles-in-innate-immune-system-of-mud-crab-scylla-paramamosain
#6
Wanwei Sun, Zhongzhen Li, Shasha Wang, Weisong Wan, Shuqi Wang, Xiaobo Wen, Huaiping Zheng, Yueling Zhang, Shengkang Li
Clip domain serine proteinases and their homologs are involved in the innate immunity of invertebrates. To identify the frontline defense molecules against pathogenic infection, we isolated a novel clip domain serine proteinase (Sp-cSP) from the hemocytes of mud crab Scylla paramamosain. The full-length 1362 bp Sp-cSP contains a 1155 bp open reading frame (ORF) encoding 384 amino acids. Multiple alignment analysis showed that the putative amino acid sequence of Sp-cSP has about 52% and 51% identity with Pt-cSP2 (AFA42360) and Pt-cSP3 (AFA42361) from Portunus trituberculatus, respectively, while the similarity with other cSP sequences was lower than 30%...
November 2015: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/26227859/molecular-characterization-tissue-and-developmental-expression-profiles-of-cryptochrome-genes-in-wing-dimorphic-brown-planthoppers-nilaparvata-lugens
#7
Jing-Jing Xu, Gui-Jun Wan, Ding-Bang Hu, Juan He, Fa-Jun Chen, Xian-Hui Wang, Hong-Xia Hua, Wei-Dong Pan
Cryptochromes (CRYs) are blue and UV light photoreceptors, known to play key roles in circadian rhythms and in the light-dependent magnetosensitivity of insects. Two novel cryptochrome genes were cloned from the brown planthopper, and were given the designations of Nlcry1 and Nlcry2, with the accession numbers KM108578 and KM108579 in GenBank. The complementary DNA sequences of Nlcry1 and Nlcry2 are 1935 bp and 2463 bp in length, and they contain an open reading frame of 1629 bp and 1872 bp, encoding amino acids of 542 and 623, with a predicted molecular weight of 62...
December 2016: Insect Science
https://www.readbyqxmd.com/read/26175462/bmhsp20-8-is-localized-in-the-mitochondria-and-has-a-molecular-chaperone-function-in-vitro
#8
Chengcheng Wu, Chan Wang, Dan Li, Yue Liu, Qing Sheng, Zhengbing Lv, Wei Yu, Zuoming Nie
Heat shock proteins (HSPs) are abundant and ubiquitous in almost all organisms from bacteria to mammals. BmHSP20.8 is a small (sHSP) in Bombyx mori that contains a 561 bp open reading frame that encodes a protein of 186 amino acid residues with a predicted molecular mass of 20.8 kDa. The subcellular localization prediction indicated that BmHSP20.8 is likely distributed in the mitochondria with a 51% probability. To identify the subcellular localization of BmHSP20.8, three recombinant vectors were constructed and used to transfect BmN cells...
2015: Journal of Insect Science
https://www.readbyqxmd.com/read/26050057/a-pilot-study-comparison-of-arm-versus-ankle-noninvasive-blood-pressure-measurement-at-2-different-levels-of-backrest-elevation
#9
Nicole Henley, Beth D Quatrara, Mark Conaway
BACKGROUND: Standard practice for obtaining noninvasive blood pressure includes arm blood pressure (BP) cuff placement at the level of the heart; however, some critical care patients cannot have BPs taken in their arm because of various conditions, and ankle BPs are frequently used as substitutes. OBJECTIVE: The aim of this study was to determine if there was a significant variation between upper arm and ankle BP measurements at different backrest elevations with consideration of peripheral edema factors...
July 2015: Dimensions of Critical Care Nursing: DCCN
https://www.readbyqxmd.com/read/26016800/de-novo-transcriptome-characterization-and-gene-expression-profiling-of-the-desiccation-tolerant-moss-bryum-argenteum-following-rehydration
#10
Bei Gao, Daoyuan Zhang, Xiaoshuang Li, Honglan Yang, Yuanming Zhang, Andrew J Wood
BACKGROUND: The desiccation-tolerant moss Bryum argenteum is an important component of the Biological Soil Crusts (BSCs) found in the Gurbantunggut desert. Desiccation tolerance is defined as the ability to revive from the air dried state. To elucidate the molecular mechanisms related to desiccation tolerance, we employed RNA-Seq and digital gene expression (DGE) technologies to study the genome-wide expression profiles of the dehydration and rehydration processes in this important desert plant...
2015: BMC Genomics
https://www.readbyqxmd.com/read/25878372/a-retrospective-study-of-the-effects-of-angiotensin-receptor-blockers-and-angiotensin-converting-enzyme-inhibitors-in-diabetic-nephropathy
#11
COMPARATIVE STUDY
Jahnavi V Pathak, Ervilla E Dass
OBJECTIVE: Till date, several studies have compared angiotensin converting enzyme (ACE) inhibitors and angiotensin receptor blockers (ARBs) in terms of delaying the progression of diabetic nephropathy. But the superiority of one drug class over the other remains unsettled. This study has retrospectively compared the effects of ACE inhibitors and ARBs in diabetic nephropathy. The study aims to compare ACE inhibitors and ARBs in terms of delaying or preventing the progression of diabetic nephropathy, association between blood pressure (B...
March 2015: Indian Journal of Pharmacology
https://www.readbyqxmd.com/read/25856419/validity-of-home-blood-pressure-measurements-manually-registered-by-patients-after-an-educational-session-provided-by-community-pharmacists
#12
José Sendra-Lillo, Fernando Martínez-Martínez, José Pedro García-Corpas, Fátima Marín Rivas, Daniel Sabater-Hernández
When home blood pressure (HBP) measurements are taken, the readings can be registered manually by the patient and/or stored in the device's memory. The instructions provided by healthcare professionals would be particularly relevant to guarantee the reliability of manual blood pressure (BP) figures and enhance clinical decision making. The aim of this study is assess the agreement between HBP measurements manually registered by patients and those stored in the device's memory after an educational session provided by community pharmacists...
August 2015: Blood Pressure Monitoring
https://www.readbyqxmd.com/read/25772963/are-bp-readings-taken-after-a-patient-physician-encounter-in-a-real-world-clinic-scenario-the-lowest-of-all-the-readings-in-a-clinic-visit
#13
Hunaina Shahab, Hamza Sohail Khan, Aysha Almas, Sohail Abrar Khan, Aamir Hameed Khan
OBJECTIVE: To determine the difference in Blood Pressure (BP) readings taken before, during and after the clinic encounter. STUDY DESIGN: Descriptive study. PLACE AND DURATION OF STUDY: Cardiology Clinic, The Aga Khan University Hospital, Karachi, from January to August 2013. METHODOLOGY: Hypertensive and normotensive participants aged ³ 18 years were recruited. Pre-clinic BP was measured by a nurse and in-clinic BP by a physician...
March 2015: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
https://www.readbyqxmd.com/read/25427456/blood-pressure-in-primary-school-children-in-uganda-a-cross-sectional-survey
#14
Farah Kidy, Diana Rutebarika, Swaib A Lule, Moses Kizza, Amos Odiit, Emily L Webb, Alison M Elliott
BACKGROUND: Non-communicable diseases are an emerging concern in sub-Saharan Africa, and risks for these conditions are often based on exposures in early life, with premonitory signs developing during childhood. The prevalence of hypertension has been reported to be high in African adults, but little is known about blood pressure in African children. We studied prevalence and risk factors for high blood pressure (HBP) among school children in central Uganda. METHODS: Two urban and five rural schools were randomly selected from government schools in Wakiso district, Uganda...
November 26, 2014: BMC Public Health
https://www.readbyqxmd.com/read/25108162/morning-cortisol-levels-in-schizophrenia-and-bipolar-disorder-a-meta-analysis
#15
Leah Girshkin, Sandra L Matheson, Alana M Shepherd, Melissa J Green
Increased peripheral levels of morning cortisol have been reported in people with schizophrenia (SZ) and bipolar disorder (BD), but findings are inconsistent and few studies have conducted direct comparisons of these disorders. We undertook a meta-analysis of studies examining single measures of morning cortisol (before 10 a.m.) levels in SZ or BD, compared to controls, and to each other; we also sought to examine likely moderators of any observed effects by clinical and demographic variables. Included studies were obtained via systematic searches conducted using Medline, BIOSIS Previews and Embase databases, as well as hand searching...
November 2014: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/24553635/how-many-clinic-bp-readings-are-needed-to-predict-cardiovascular-events-as-accurately-as-ambulatory-bp-monitoring
#16
K Eguchi, S Hoshide, K Shimada, K Kario
We tested the hypothesis that multiple clinic blood pressure (BP) readings over an extended baseline period would be as predictive as ambulatory BP (ABP) for cardiovascular disease (CVD). Clinic and ABP monitoring were performed in 457 hypertensive patients at baseline. Clinic BP was measured monthly and the means of the first 3, 5 and 10 clinic BP readings were taken as the multiple clinic BP readings. The subjects were followed up, and stroke, HARD CVD, and ALL CVD events were determined as outcomes. In multivariate Cox regression analyses, ambulatory systolic BP (SBP) best predicted three outcomes independently of baseline and multiple clinic SBP readings...
December 2014: Journal of Human Hypertension
https://www.readbyqxmd.com/read/24508618/identification-and-functional-characterization-of-heat-shock-transcription-factor1-in-litopenaeus-vannamei
#17
Hui Yan, Shuang Zhang, Xiao-Yun Li, Feng-Hua Yuan, Wei Qiu, Yong-Gui Chen, Shao-Ping Weng, Jian-Guo He, Yi-Hong Chen
Heat shock transcription factors belong to the heat shock factor (HSF) protein family, which are involved in heat shock protein (HSP) gene regulation. They are critical for cell survival upon exposure to harmful conditions. In this study, we identified and characterized a HSF1 (LvHSF1) gene in Litopenaeus vannamei, with a full-length cDNA of 2841 bp and an open reading frame encoding a putative protein of 632 amino acids. Through multiple sequence alignment and phylogenetic analysis, it was revealed that LvHSF1 was closed to insect HSF family, which contained a highly conserved DNA-binding domain, oligomerization domains with HR-A/B, and a nuclear localization signal...
March 2014: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/24475551/hypertensive-leucocytosis
#18
Rolinda Rajkumari, Deben Laishram, Joshna Thiyam, Ng Javan
There are studies showing association of high WBC count with the higher incidence of hypertension though a few are done in the Indian population. The present study was conducted with the view to find any significant increase in total leucocyte count and differential leucocyte count in hypertensive patient Twenty-seven hypertensives with 12 males and 15 females and 27 age and sex matched control subjects (normotensive) were studied. Hypertension was defined when the systolic BP > or = 140 mmHg or diastolic BP > or = 90 mmHg or history of taking antihypertensive medicine...
April 2013: Journal of the Indian Medical Association
https://www.readbyqxmd.com/read/23819734/replacing-manual-sphygmomanometers-with-automated-blood-pressure-measurement-in-routine-clinical-practice
#19
REVIEW
Martin G Myers
1. Conventional manual measurement of blood pressure (BP) in clinical practice is no longer considered to be the best method for evaluating a patient's BP status. Home BP and 24 h ambulatory BP monitoring are now recommended for the diagnosis and management of hypertension.  2. Recent studies provide an alternative to conventional office BP, namely automated office (AO) BP, which involves multiple BP readings taken with a fully automated device with the patient resting quietly alone. Automated office BP is preferable to routine manual office BP in that it exhibits improved accuracy and a stronger relationship to both ambulatory BP and target organ damage...
January 2014: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/23380961/a-novel-peroxidase-canpod-gene-of-pepper-is-involved-in-defense-responses-to-phytophtora-capsici-infection-as-well-as-abiotic-stress-tolerance
#20
Jun-E Wang, Ke-Ke Liu, Da-Wei Li, Ying-Li Zhang, Qian Zhao, Yu-Mei He, Zhen-Hui Gong
Peroxidases are involved in many plant processes including plant defense responses to biotic and abiotic stresses. We isolated a novel peroxidase gene CanPOD from leaves of pepper cultivar A3. The full-length gene has a 1353-bp cDNA sequence and contains an open reading frame (ORF) of 975-bp, which encodes a putative polypeptide of 324 amino acids with a theoretical protein size of 34.93 kDa. CanPOD showed diverse expression levels in different tissues of pepper plants. To evaluate the role of CanPOD in plant stress responses, the expression patterns of CanPOD were examined using Real-Time RT-PCR...
2013: International Journal of Molecular Sciences
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