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https://www.readbyqxmd.com/read/28110484/upregulation-of-20-hete-synthetic-cytochrome-p450-isoforms-by-oxygen-glucose-deprivation-in-cortical-neurons
#1
Hui Zhang, John R Falck, Richard J Roman, David R Harder, Raymond C Koehler, Zeng-Jin Yang
20-Hydroxyeicosatetraenoic acid (20-HETE), a potent vasoconstrictor, is a cytochrome P450 (CYP) 4A/4F-derived metabolite of arachidonic acid. Inhibition of 20-HETE synthesis protects brain from ischemic injury. However, that protection is not associated with changes in cerebral blood flow. The present study examined whether CYP4A isoforms are expressed in neurons, whether they produce 20-HETE in neurons, and whether neuronally derived 20-HETE exerts direct neurotoxicity after oxygen-glucose deprivation (OGD)...
January 21, 2017: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/28110426/influenza-a-virus-upregulates-prpf8-gene-expression-to-increase-virus-production
#2
Chee-Hing Yang, Hui-Chun Li, Yu-Ling Shiu, Tzu-Shan Ku, Chia-Wen Wang, Yi-Shuan Tu, Hung-Ling Chen, Cheng-Hao Wu, Shih-Yen Lo
A ddRT-PCR analysis was performed to detect cellular genes that are differentially expressed after influenza A virus (H1N1) infection of A549 cells. After ddRT-PCR, eight DNA fragments were identified. PRPF8, one of the cellular genes that were upregulated after virus infection, was further analyzed since it has previously been identified as a cellular factor required for influenza virus replication. The upregulation of PRPF8 gene expression after viral infection was confirmed using real-time RT-PCR for mRNA detection and Western blot analysis for protein detection...
January 21, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28110264/novel-phyto-derivative-brm270-inhibits-hepatocellular-carcinoma-cells-proliferation-by-inducing-g2-m-phase-cell-cycle-arrest-and-apoptosis-in-xenograft-mice-model
#3
Raj Kumar Mongre, Neelesh Sharma, Simrinder Singh Sodhi, Mrinmoy Ghosh, Amit Kumar Singh, Nameun Kim, Yang Ho Park, Young Gyu Shin, Sung Jin Kim, Zhang Jiao Jiao, Do Luong Huynh, Dong Kee Jeong
Hepatocellular carcinoma (HCC) is a major threat to human health worldwide and development of novel antineoplastic drug is demanding task. BRM270 is a proprietary combination of traditional medicinal herbs, has been shown to be effective against a wide range of stem-like cancer initiating cells (SLCICs). However, the underlying mechanism and antitumor efficacy of BRM270 in human hepatocellular carcinoma (HCC) cells have not been well elucidated till date. Here we studied the tumoricidal effect of BRM270 on human-CD133(+) expressing stem-like HepG-2 and SNU-398 cells...
January 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28110191/asiatic-acid-enhances-nrf2-signaling-to-protect-hepg2-cells-from-oxidative-damage-through-akt-and-erk-activation
#4
Zhimin Qi, Xinxin Ci, Jingbo Huang, Qinmei Liu, Qinlei Yu, Junfeng Zhou, Xuming Deng
Asiatic acid (AA), a natural triterpene isolated from the plant Centella asiatica, have antioxidative potential, but the molecular mechanism of AA against oxidative stress remains unclear. Our study was performed to investigate the antioxidative effect of AA against oxidative stress and the antioxidative mechanism in tert-butyl hydroperoxide (t-BHP) -stimulated the HepG2 cells. The results showed that AA suppressed t-BHP-induced cytotoxicity, apoptosis, and reactive oxygen species (ROS) generation. Additionally, AA activated the nuclear factor erythroid 2-related factor 2 (Nrf2) signal, which was closely related to induction Nrf2 nuclear translocation, reduction the expression of Keap1 and up-regulation the activity of the antioxidant response element (ARE)...
January 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28110074/covalently-immobilized-vegf-mimicking-peptide-with-gelatin-methacrylate-enhances-microvascularization-of-endothelial-cells
#5
S Prakash Parthiban, Deepti Rana, Esmaiel Jabbari, Nadia Benkirane-Jessel, Murugan Ramalingam
: Clinically usable tissue-engineered constructs are currently limited due to their inability of forming microvascular networks necessary for adequate cellular oxygen and nutrient supply upon implantation. The aim of this study is to investigate the conditions necessary for microvascularization in a tissue-engineered construct using vascular endothelial growth factor (VEGF). The construct was made of gelatin methacrylate (GelMA) based cell-laden hydrogel system, which was then covalently linked with VEGF-mimicking peptide (AcQK), using human umbilical vein endothelial cells (HUVECs) as the model cell...
January 18, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28110067/poly-glycerol-sebacate-elastomer-supports-bone-regeneration-by-its-mechanical-properties-being-closer-to-osteoid-tissue-rather-than-to-mature-bone
#6
S H Zaky, K W Lee, J Gao, A Jensen, K Verdelis, Y Wang, A J Almarza, C Sfeir
: Mechanical load influences bone structure and mass. Arguing the importance of load-transduction, we investigated the mechanisms inducing bone formation using an elastomeric substrate. We characterized Poly (glycerol sebacate) (PGS) in-vitro for its mechanical properties, compatibility with osteoprogenitor cells regarding adhesion, proliferation, differentiation under compression versus static cultures and in-vivo for the regeneration of a rabbit ulna critical size defect. The load-transducing properties of PGS were compared in-vitro to a stiffer poly lactic-co- glycolic-acid (PLA/PGA) scaffold of similar porosity and interconnectivity...
January 18, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28109981/thyroid-hormone-suppresses-ischemia-reperfusion-induced-liver-nlrp3-inflammasome-activation-role-of-amp-activated-protein-kinase
#7
Romina Vargas, Luis A Videla
Thyroid hormone (T3) induces liver preconditioning (PC) against ischemia-reperfusion (IR), a response energetically supported by AMP-activated protein kinase (AMPK) upregulation. The aim of this work is to evaluate the influence of T3 on IR-induced liver NLRP3 inflammasome activation and the relevance of AMPK activity on liver injury by the use of the AMPK inhibitor compound C (CC). Male Sprague-Dawley rats were given 0.1mgT3/kg (time zero) and 10mg CC/kg (time zero and 24h) or the respective vehicles, and subjected to 1h ischemia-20h reperfusion 48h after hormone treatment...
January 18, 2017: Immunology Letters
https://www.readbyqxmd.com/read/28109806/momordica-charantia-polysaccharides-ameliorate-oxidative-stress-hyperlipidemia-inflammation-and-apoptosis-during-myocardial-infarction-by-inhibiting-the-nf-%C3%AE%C2%BAb-signaling-pathway
#8
Mohammad Raish
The polysaccharide extract of Momordica charantia has various biological activities; however, its effect on endothelial dysfunction in myocardial infarction remains unclear. To elucidate this, myocardial infarction was induced in rats using isoproterenol (ISP). Pretreatment with M. charantia polysaccharides (MCP; 150 or 300mg/kg) for 25days significantly inhibited increases in heart weight, the heart-weight-to-body-weight ratio, and infarction size, and ameliorated the increased serum levels of aspartate transaminase, creatine kinase, lactate dehydrogenase, total cholesterol, triglycerides, very-low-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol...
January 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28109721/inflammasome-priming-in-sterile-inflammatory-disease
#9
REVIEW
Meghana N Patel, Richard G Carroll, Silvia Galván-Peña, Evanna L Mills, Robin Olden, Martha Triantafilou, Amaya I Wolf, Clare E Bryant, Kathy Triantafilou, Seth L Masters
The inflammasome is a cytoplasmic protein complex that processes interleukins (IL)-1β and IL-18, and drives a form of cell death known as pyroptosis. Oligomerization of this complex is actually the second step of activation, and a priming step must occur first. This involves transcriptional upregulation of pro-IL-1β, inflammasome sensor NLRP3, or the non-canonical inflammasome sensor caspase-11. An additional aspect of priming is the post-translational modification of particular inflammasome constituents...
January 18, 2017: Trends in Molecular Medicine
https://www.readbyqxmd.com/read/28109598/identification-and-analysis-of-the-expression-of-microrna-from-lactating-and-nonlactating-mammary-glands-of-the-chinese-swamp-buffalo
#10
Xiaoyan Cai, Qingyou Liu, Xiaoxi Zhang, Yanping Ren, Xiaocan Lei, Sheng Li, Qiuping Chen, Kai Deng, Ping Wang, Haihang Zhang, Deshun Shi
To study the role of microRNA (miR) in the lactation physiology of water buffalo, 2 multiparous dairy buffaloes (including an 8-yr-old buffalo that had been lactating for 3 mo, as well as a 10-yr-old nonlactating, nonpregnant buffalo) were used for miR library construction. The profile of differentially expressed miR in lactating and nonlactating mammary gland tissues of these water buffalo were investigated using Illumina-Solexa high-throughput sequencing technology (Illumina, San Diego, CA). The data identified 259 miR families, 359 mature miR, 363 pre-miR, 230 novel buffalo miR, and 5 buffalo-specific miR that were expressed in mammary tissues...
January 18, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28109445/identification-of-novel-macropinocytosing-human-antibodies-by-phage-display-and-high-content-analysis
#11
K D Ha, S M Bidlingmaier, Y Su, N-K Lee, B Liu
Internalizing antibodies have great potential for the development of targeted therapeutics. Antibodies that internalize via the macropinocytosis pathway are particularly promising since macropinocytosis is capable of mediating rapid, bulk uptake and is selectively upregulated in many cancers. We hereby describe a method for identifying antibodies that internalize via macropinocytosis by screening phage-displayed single-chain antibody selection outputs with an automated fluorescent microscopy-based high-content analysis platform...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28109308/semaphorin-7a-as-a-potential-immune-regulator-and-promising-therapeutic-target-in-rheumatoid-arthritis
#12
Jianmin Xie, Hao Wang
BACKGROUND: Semaphorin 7A (Sema7A) is expressed by several different classes of lymphoid and myeloid cells and is a potent immunomodulator. We examined the role of Sema7A in modulating cellular immune responses and to provide experimental data validating the therapeutic potential of Sema7A in rheumatoid arthritis (RA). METHODS: Soluble Sema7A (sSema7A) levels in the serum and synovial fluid from patients with RA or osteoarthritis, as well as cytokine secretions, were analyzed with an enzyme-linked immunosorbent assay...
January 21, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28109307/novel-roles-of-dc-signr-in-colon-cancer-cell-adhesion-migration-invasion-and-liver-metastasis
#13
Heya Na, Xiaoli Liu, Xiaomeng Li, Xinsheng Zhang, Yu Wang, Zhaohui Wang, Menglang Yuan, Yu Zhang, Shuangyi Ren, Yunfei Zuo
BACKGROUND: Tumor metastasis is an essential cause of the poor prognosis of colon cancer. DC-SIGNR is a C-type lectin that is frequently found on human liver sinusoidal endothelial cells. LSECtin, which is a homologue of DC-SIGNR, has been demonstrated to participate in colon cancer liver metastasis. Due to the similarities in the expression pattern and structure of the two proteins, we speculated that DC-SIGNR could also be involved in this process. METHODS: Colon cancer cells were treated with the DC-SIGNR protein or control IgG, after which cell migration, invasion, and morphology were assayed...
January 21, 2017: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/28109288/long-intergenic-non-coding-rna-00152-promotes-lung-adenocarcinoma-proliferation-via-interacting-with-ezh2-and-repressing-il24-expression
#14
Qin-Nan Chen, Xin Chen, Zhen-Yao Chen, Feng-Qi Nie, Chen-Chen Wei, Hong-Wei Ma, Li Wan, Shuai Yan, Sheng-Nan Ren, Zhao-Xia Wang
BACKGROUND: Numerous studies have shown that long non-coding RNAs (lncRNAs) behave as a novel class of transcript during multiple cancer processes, such as cell proliferation, apoptosis, migration, and invasion. LINC00152 is located on chromosome 2p11.2, and has a transcript length of 828 nucleotides. The biological role of LINC00152 in LAD(lung adenocarcinoma) remains unknown. METHODS: Quantitative reverse transcription PCR(qRT-PCR) was used to detect LINC00152 expression in 60 human LAD tissues and paired normal tissues...
January 21, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28109175/langerhans-cell-markers-cd1a-and-cd207-are-the-most-rapidly-responding-genes-in-lesional-psoriatic-skin-following-adalimumab-treatment
#15
L Raaby, C Rosada, A Langkilde, K L Lauridsen, H Vinter, P Ommen, R B Kjellerup, C Johansen, L Iversen
TNFα-, IL-23- and IL-17-targeting drugs are highly effective in the treatment of psoriasis. However, the precise molecular mechanism remains unknown. In psoriatic skin the presence of Langerhans cells (LC) is reduced, but the role of LC is poorly understood. The purpose of this study was to investigate the impact of TNFα and IL-23/IL-17 on the presence of LC in the skin during treatment. Therefore, psoriatic skin was investigated before and after 4 days of adalimumab or ustekinumab treatment. Furthermore, TNFα and IL-17A stimulation was investigated in an ex vivo model of epidermis and dermis from healthy normal skin kept in cultures at an air-liquid interphase for 4 days...
January 21, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28109073/cyclin-dependent-kinase-inhibitor-3-promotes-cancer-cell-proliferation-and-tumorigenesis-in-nasopharyngeal-carcinoma-by-targeting-p27
#16
Huimin Wang, Hexin Chen, Hang Zhou, Wenfa Yu, Zhenmin Lu
Nasopharyngeal carcinoma (NPC) is a common head and neck malignancy with high invasion which arises from the nasopharynx epithelium. Cyclin-dependent kinase inhibitor 3 (CDKN3) belongs to the dual-specificity protein phosphatase family which plays a key role in regulating cell division. Abnormal expression of CDKN3 has been found in numerous types of cancer. In the current study, we explored the possible role of CDKN3 in cell proliferation, invasion ability and radiosensitivity in NPC cells. We reported that CDKN3 was upregulated and P27 was downregulated in NPC tissues and associated with worse patient prognosis...
January 20, 2017: Oncology Research
https://www.readbyqxmd.com/read/28109039/nkx2-5-regulates-endothelin-converting-enzyme-1-during-pharyngeal-arch-patterning
#17
Jennifer M Iklé, Andre L P Tavares, Marisol King, Hailei Ding, Sophie Colombo, Beth A Firulli, Anthony B Fiulli, Kimara L Targoff, Deborah Yelon, David E Clouthier
In gnathostomes, dorsoventral (D-V) patterning of neural crest cells (NCC) within the pharyngeal arches is crucial for the development of hinged jaws. One of the key signals that mediates this process is Endothelin-1 (EDN1). Loss of EDN1 binding to the Endothelin-A receptor (EDNRA) results in loss of EDNRA signaling and subsequent facial birth defects in humans, mice and zebrafish. A rate-limiting step in this crucial signaling pathway is the conversion of immature EDN1 into a mature active form by Endothelin converting enzyme-1 (ECE1)...
January 20, 2017: Genesis: the Journal of Genetics and Development
https://www.readbyqxmd.com/read/28108976/phenotypic-and-biochemical-alterations-in-relation-to-mt2-gene-expression-in-plantago-ovata-forsk-under-zinc-stress
#18
Paulami Pramanick, Anindita Chakraborty, Sarmistha Sen Raychaudhuri
Plantago ovata Forsk is an annual herb with immense medicinal importance, the seed and husk of which is used in the treatment of chronic constipation, irritable bowel syndrome, diarrhea since ancient times. Zinc, an essential metal, is required by plants as they form important components of zinc finger proteins and also aid in synthesis of photosynthetic pigments such as chlorophyll. However, in excess amount Zn causes chlorosis of leaf and shoot tissues and generate reactive oxygen species. The present study is aimed at investigating the changes in expression levels of MT2 gene in Plantago ovata under zinc stress...
January 20, 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/28108744/increased-breast-cancer-risk-with-habp1-p32-gc1qr-genetic-polymorphism-rs2285747-and-its-upregulation-in-northern-chinese-women
#19
Yongdong Jiang, Hao Wu, Jing Liu, Yanbo Chen, Jingjing Xie, Yashuang Zhao, Da Pang
OBJECT: Hyaluronic acid binding protein 1 (HABP1/p32/gC1qR) is overexpressed in breast cancer. However, it is unknown whether HABP1 gene polymorphisms affect breast cancer risk. This study aims to evaluate the potential association of single nucleotide polymorphisms (SNPs) of HABP1 with breast cancer in northern Chinese women. RESULTS: The minor allele of rs2285747 was strongly associated with breast cancer with OR of 1.553 (95% CI = 1.251-1.927). SNP rs2285747 was also associated with high HABP1 protein expression under the co-dominant and dominant model (p = 0...
January 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/28108734/valproic-acid-inhibits-glioblastoma-multiforme-cell-growth-via-paraoxonase-2-expression
#20
Jen-Ho Tseng, Cheng-Yi Chen, Pei-Chun Chen, Sheng-Huang Hsiao, Chi-Chen Fan, Yu-Chih Liang, Chie-Pein Chen
We studied the potential mechanisms of valproic acid (VPA) in the treatment of glioblastoma multiforme (GBM). Using the human U87, GBM8401, and DBTRG-05MG GBM-derived cell lines, VPA at concentrations of 5 to 20 mM induced G2/M cell cycle arrest and increased the production of reactive oxygen species (ROS). Stress-related molecules such as paraoxonase 2 (PON2), cyclin B1, cdc2, and Bcl-xL were downregulated, but p27, p21 and Bim were upregulated by VPA treatment. VPA response element on the PON2 promoter was localized at position -400/-1...
January 18, 2017: Oncotarget
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