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Flowcytometry hematology

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https://www.readbyqxmd.com/read/27766226/nf-k%C3%AE-activation-in-u266-cells-on-mesenchymal-stem-cells
#1
Sara Zahedi, Karim Shamsasenjan, Aliakbar Movassaghpour, Parvin Akbarzadehlaleh
Purpose: Mesenchymal Stem Cells (MSCs) are one of the essential members of Bone Marrow (BM) microenvironment and the cells affect normal and malignant cells in BM milieu. One of the most important hematological malignancies is Multiple Myeloma (MM). Numerous studies reported various effects of MSCs on myeloma cells. MSCs initiate various signaling pathways in myeloma cells, particularly NF-kβ. NF-kβ signaling pathway plays pivotal role in the survival, proliferation and resistance of myeloma cells to the anticancer drugs, therefore this pathway can be said to be a vital target for cancer therapy...
September 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/27635203/effects-of-valproic-acid-and-pioglitazone-on-cell-cycle-progression-and-proliferation-of-t-cell-acute-lymphoblastic-leukemia-jurkat-cells
#2
Marie Saghaeian Jazi, Saeed Mohammadi, Yaghoub Yazdani, Sima Sedighi, Ali Memarian, Mehrdad Aghaei
OBJECTIVES: T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematologic malignant tumor. Administration of chemical compounds influencing apoptosis and T cell development has been discussed as promising novel therapeutic strategies. Valproic acid (VPA) as a recently emerged anti-neoplastic histone deacetylase (HDAC) inhibitor and pioglitazone (PGZ) as a high-affinity peroxisome proliferator-activated receptor-gamma (PPARγ) agonist have been shown to induce apoptosis and cell cycle arrest in different studies...
July 2016: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/25960120/a-comparison-of-the-immunological-effects-of-propofol-and-isoflurane-for-maintenance-of-anesthesia-in-healthy-dogs
#3
RANDOMIZED CONTROLLED TRIAL
Mizuki Tomihari, Akira Nishihara, Terumasa Shimada, Masashi Yanagawa, Masafumi Miyoshi, Kazurou Miyahara, Akihiro Oishi
Most anesthetics have an immuno-suppressive effect on cellular and neurohumoral immunity, and research shows that total intravenous anesthesia (TIVA) with propofol has a greater immuno-protective effect than inhalational anesthesia in human medicine. However, in veterinary clinics, these effects remain ambiguous. To clarify the details, we focused on propofol and isoflurane, investigating clinical blood hematology and immunological profiles drawn from healthy dogs under and after two anesthesia techniques. Twelve healthy adult beagles were included in this study, randomly assigned to the propofol anesthesia group (group P: n=6) or the isoflurane anesthesia group (group I: n=6)...
October 2015: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/25538404/hairy-cell-leukemia-a-decade-long-experience-of-north-indian-hematology-center
#4
Venkatesan Somasundaram, Abhishek Purohit, Mukul Aggarwal, Prabhu Manivannan, Pravas Mishra, Tulika Seth, Seema Tyagi, Manoranjan Mahapatra, Hara P Pati, Renu Saxena
INTRODUCTION: Hairy cell leukemia is a rare chronic B-cell disorder that follows an indolent but progressive course. This disorder is characterized by pancytopenia, splenomegaly, bone marrow fibrosis and the presence of atypical lymphoid cells with hairy projections in peripheral blood, bone marrow and spleen. Treatment is mainly with nucleoside analog cladribine, which induces complete remission in up to 85% cases. MATERIALS AND METHODS: This is a retrospective analysis of Hairy cell Leukemia cases diagnosed and treated in the Department of Hematology, All India Institute of Medical Sciences, New Delhi between 2002 and 2013...
October 2014: Indian Journal of Medical and Paediatric Oncology
https://www.readbyqxmd.com/read/25157470/synthesis-and-biological-evaluation-of-new-pyridone-annelated-isoindigos-as-anti-proliferative-agents
#5
Ayman M Saleh, Randa M Al-As'ad, Mustafa M El-Abadelah, Salim S Sabri, Jalal A Zahra, Ahmed S Alaskar, Ahmad Aljada
A selected set of substituted pyridone-annelated isoindigos 3a-f has been synthesized via interaction of 5- and 6-substituted oxindoles 2a-f with 6-ethyl-1,2,9-trioxopyrrolo[3,2-f]quinoline-8-carboxylic acid (1) in acetic acid at reflux. Among these isoindigos, the 5'-chloro and 5'-bromo derivatives 3b and 3d show strong and selective antiproliferative activities against a panel of human hematological and solid tumor cell-lines, but not against noncancerous cells, suggesting their potential use as anticancer agents...
August 25, 2014: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/24284303/production-and-characterization-of-murine-monoclonal-antibody-against-synthetic-peptide-of-cd34
#6
REVIEW
Leili Aghebati Maleki, Jafar Majidi, Behzad Baradaran, Jalal Abdolalizadeh, Aliakbar Movassaghpour Akbari
BACKGROUND: The treatment of hematologic malignancies and immunodeficiency diseases are offered by hematopoietic stem cells (HSCs) as a unique self-renewal and differentiation source which most commonly is selected by CD34 surface marker for HSC. OBJECTIVE: The purpose of this study was to develop and characterize monoclonal antibody against CD34 antigen for detection of hematopoietic stem cells. METHODS: Balb/c mice were immunized with two synthetic peptides of CD34 and Spleen cells were fused with SP2/0...
2013: Human Antibodies
https://www.readbyqxmd.com/read/24146762/deferred-pre-emptive-switch-from-calcineurin-inhibitor-to-sirolimus-leads-to-improvement-in-gfr-and-expansion-of-t-regulatory-cell-population-a-randomized-controlled-trial
#7
RANDOMIZED CONTROLLED TRIAL
Dinesh Bansal, Ashok K Yadav, Vinod Kumar, Mukut Minz, Vinay Sakhuja, Vivekanand Jha
BACKGROUND: Measures to prevent chronic calcineurin inhibitor (CNI) toxicity have included limiting exposure by switching to sirolimus (SIR). SIR may favorably influence T regulator cell (T(reg)) population. This randomized controlled trial compares the effect of switching from CNI to SIR on glomerular filtration rate (GFR) and T(reg) frequency. METHODS: In this prospective open label randomized trial, primary living donor kidney transplant recipients on CNI-based immunosuppression were randomized to continue CNI or switched to sirolimus 2 months after surgery; 29 were randomized to receive CNI and 31 to SIR...
2013: PloS One
https://www.readbyqxmd.com/read/23633866/the-effects-of-residence-duration-in-high-background-radiation-areas-on-immune-surveillance
#8
Sajad Borzoueisileh, Ali Shabestani Monfared, Saeid Abediankenari, Amrollah Mostafazadeh, Meysam Khosravifarsani
PURPOSE: The effective dose received by humans from natural sources is about 2.4 mSv y(-1), but this is 10.2 mSv y(-1) for inhabitants of Ramsar, a city in northern Iran. Carcinogenesis is one of the most studied effects of radiation, especially in high doses. Nonetheless carcinogenesis of low doses is uncertain. A recent epidemiological study in high background radiation areas of Ramsar showed that the cancer incidence in this era is lower than neighbors. The reason of this different behavior is under study yet...
January 2013: Journal of Natural Science, Biology, and Medicine
https://www.readbyqxmd.com/read/22733614/flt3-and-npm1-mutations-in-a-cohort-of-aml-patients-and-detection-of-a-novel-mutation-in-tyrosine-kinase-domain-of-flt3-gene-from-western-india
#9
Kanjaksha Ghosh, Suchitra Swaminathan, Manisha Madkaikar, Maya Gupta, Lily Kerketta, Baburao Vundinti
Acute myeloid leukemia (AML) is an aggressive hematological disorder characterized by the loss of ability of the hematopoietic progenitor cells to differentiate and proliferate normally leading to an accumulation of immature myeloid cells in the bone marrow. Several novel molecular genetic aberrations in FLT3 and NPM1 have been shown to have a prognostic impact in AML, particularly in those having normal karyotype. Though there is substantial amount of data on these mutations from western literature, there is surprisingly little data from Indian subcontinent on the frequency of this mutation in AML patients from India...
November 2012: Annals of Hematology
https://www.readbyqxmd.com/read/22674250/superior-mediastinal-syndrome-a-rare-presenting-feature-of-acute-myeloid-leukemia
#10
Rachna Seth, Rishi Bolia, Richa Jain, Anita Chopra, Saroj Singh, Rajive Kumar
Superior mediastinal syndrome (SMS) is an uncommon manifestation of malignant neoplastic disease in children. The commonest neoplastic cause of SMS is Non Hodgkin lymphoma. Acute myeloid leukemia (AML) as a cause of SMS is extremely uncommon in childhood. The authors hereby report a case of a child with AML who presented with SMS at their hospital. This report also highlights the importance of flowcytometry as a diagnostic modality in hematological malignancies.
February 2013: Indian Journal of Pediatrics
https://www.readbyqxmd.com/read/22379290/prognostic-significance-of-survivin-in-pediatric-acute-lymphoblastic-leukemia
#11
Asmaa M Esh, Maha Atfy, Nashwa A Azizi, Mohamed M El Naggar, Ebtesam E Khalil, Layla Sherief
Impaired apoptosis is mediated by members of the inhibitor of apoptosis proteins (IAP) family such as survivin. Survivin was described in number of different tumors and found to correlate with poor prognosis in a variety of cancers including hematologic malignancies. The aim of this study was to determine survivin in pediatric ALL and compare it with clinical and hematological findings, response to therapy and outcome. Flowcytometry was used for detection of intracellular survivin and determine its mean fluorescence intensity (MFI) in bone marrow mononuclear cells...
March 2011: Indian Journal of Hematology & Blood Transfusion
https://www.readbyqxmd.com/read/20600326/murine-bone-marrow-chimeras-developing-autoimmunity-after-ctla-4-blockade-show-an-expansion-of-t-regulatory-cells-with-an-activated-cytokine-profile
#12
Lien De Somer, Sabine Fevery, Dominique M A Bullens, Omer Rutgeerts, Caroline Lenaerts, Chantal Mathieu, Louis Boon, Ahmad Kasran, Mark Waer, An D Billiau
Autoimmune adverse events are a concern in patients treated with blocking anti-CTLA-4-mAb for solid and hematological tumors. Patient and mouse data on the contribution of a quantitative or qualitative defect of regulatory T cells (T(reg)) in this autoimmune phenomenon are conflicting. We have previously shown that a treatment course with blocking anti-CTLA-4-mAb in murine allogeneic bone marrow chimeras induces an antileukemic response in close association with systemic autoimmunity. Here, we used this model to investigate the effect of CTLA-4-blocking therapy on the kinetics of T(reg) frequency and function...
September 6, 2010: Immunology Letters
https://www.readbyqxmd.com/read/20229505/comparison-of-platelet-counts-by-celldyn-sapphire-abbot-diagnostics-lh750-beckman-coulter-reapanthrombo-immunoplatelet-method-reametrix-and-the-international-flow-reference-method-in-thrombocytopenic-blood-samples
#13
COMPARATIVE STUDY
Kunal Sehgal, Y Badrinath, Prashant Tembhare, P G Subramanian, Sanjay Talole, Ashok Kumar, Vijaya Gadage, Shashikant Mahadik, Sitaram Ghogale, Sumeet Gujral
BACKGROUND: We compared the international flow reference method (IRM) platelet counts with those obtained from CellDyn Sapphire (impedance and optical counts), LH750 (impedance counts), and the flowcytometry based ReaPanThrombo Immunoplatelet method (ReaMetrix). We further evaluated the degree of agreement of above methods with the IRM at the transfusion thresholds of 10 x 10(9) l(-1) and 20 x 10(9) l(-1). METHODS: A total of 104 thrombocytopenic blood samples with platelet count of <50 x 10(9) l(-1) were selected for the study...
July 2010: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/20067170/splenectomy-a-therapeutic-option-in-splenic-marginal-zone-cell-lymphoma
#14
Ana-Maria Vlădăreanu, Minodora Onisâi, Cristina Ciufu, H Bumbea, Diana Cîşleanu, Irina Voican, Anca Nicolescu, Sînziana Radeşi, Anamaria Vintilescu, Cristina Băluţă, Camelia Dobrea, C Savlovschi, L Grecu, D Serban, G Serafim
We present the case of a 65 years old male, admitted in the Hematology Department of the Universitary Emergency Hospital Bucharest, complaining of physical asthenia and weight loss; periodical medical examination has revealed splenomegaly and leucocytosis with lymphocytosis, persistent for the past 3 years. The clinical and paraclinical exam demonstrated splenomegaly (21 cm in diameter on computer tomography scan), hepatomegaly and generalized lymphadenopathies. The laboratory tests confirmed leucocytosis with lymphocytosis--a clonal population of B lymphocytes CD20+ CD19+ CD23+/- CD79b+(low), CD43+ FMC7+ CD5+ CD38+ ZAP70+ cyclin D1-...
2009: Romanian Journal of Internal Medicine, Revue Roumaine de Médecine Interne
https://www.readbyqxmd.com/read/19678930/profile-of-hematological-abnormalities-of-indian-hiv-infected-individuals
#15
Byomakesh Dikshit, Ajay Wanchu, Ravinder Kaur Sachdeva, Aman Sharma, Reena Das
BACKGROUND: Hematological abnormalities are a common complication of HIV infection. These abnormalities increase as the disease advances. Bone marrow abnormalities occur in all stages of HIV infection. METHODS: Two hundred HIV infected individual were screened for hematological abnormalities from March 2007-March 2008. Absolute CD4 cell count analysis was carried out by flowcytometry. Depending on the results of the primary screening further investigations were performed, like iron studies, hemolytic work up, PNH work up and bone marrow evaluation...
2009: BMC Blood Disorders
https://www.readbyqxmd.com/read/17996672/effects-of-developmental-hypothyroidism-induced-by-maternal-administration-of-methimazole-or-propylthiouracil-on-the-immune-system-of-rats
#16
Ryosuke Nakamura, Reiko Teshima, Akiko Hachisuka, Yuji Sato, Kayoko Takagi, Rika Nakamura, Gye-Hyeong Woo, Makoto Shibutani, Jun-Ichi Sawada
Methimazole (MMI) and propylthiouracil (PTU) are popularly used antithyroid drugs (ATDs) for the treatment of Graves' hyperthyroidism. The aim of the present study was to determine the effects of ATDs on the developing immune system of the rats. Maternal Sprague-Dawley rats were given drinking water containing 200 ppm of MMI, 12 ppm of PTU (high-dose PTU), or 3 ppm of PTU (low-dose PTU) between gestational day (GD) 10 and postnatal week (PNW) 3. Exposure to the ATDs was ceased upon weaning at PNW3, and the male offspring were sampled at PNWs 3 or 11...
December 15, 2007: International Immunopharmacology
https://www.readbyqxmd.com/read/17365856/microparticle-associated-p-selectin-reflects-platelet-activation-in-preeclampsia
#17
Christine A R Lok, Rienk Nieuwland, Augueste Sturk, Chi M Hau, Kees Boer, Ed Vanbavel, Joris A M Vanderpost
Platelet activation in preeclampsia is reflected by elevated levels of platelets exposing P-selectin. In plasma, a non-cell bound (soluble) form of P-selectin is present. Elevated levels of this soluble form have been reported in preeclampsia. Plasma P-selectin may consist of two fractions: microparticle (MP)--associated P-selectin and non-MP--associated P-selectin. In the present cross-sectional study, we investigated to which extent plasma P-selectin is MP--associated and whether such MP are elevated in preeclamptic patients...
February 2007: Platelets
https://www.readbyqxmd.com/read/17301357/p-glycoprotein-quantitation-in-acute-leukemia
#18
Mahin Nikougoftar, M Farhadi, A A Pourfathollah
Multi drug resistance(MDR) is a major problem in the treatment ofcancer and hematological malignancies. This resistance is multi factorial and is the result of decreased intra cellular drug accumulation. This is partly due to the presence of a 170KD intra membranous protein termed P-glycoprotein(P-gp) that is an energy- dependent efflux pump which has increased expression on drug-resistance cells. In this study we identified the presence of P-gp by staining with Fluorescent Iso Thio Cyanate (FITC) conjugated anti P-gp in acute leukemia patients and flow cytometry in addition to performing immunophenotype analysis and French, American British (FAB) classification...
June 2003: Iranian Journal of Allergy, Asthma, and Immunology
https://www.readbyqxmd.com/read/16197480/flowcytometric-analysis-of-basophil-counts-in-human-blood-and-inaccuracy-of-hematology-analyzers
#19
S Ducrest, F Meier, C Tschopp, R Pavlovic, C A Dahinden
BACKGROUND: Differential leukocyte counts are of proven clinical value, but information about basophil counts in normal and disease conditions is scarce although basophils are regarded as key effector cells in allergy. AIMS: To establish and validate flowcytometric methods for counting basophils in peripheral human blood, to determine reference values, and to examine the accuracy of two widely used hematology analyzers. METHODS: Basophils were measured in whole blood by flowcytometry after staining with antibodies against the IL-3-receptor (CD123) or the eotaxin-receptor (CCR3) combined with other markers used for gating or validation purposes...
November 2005: Allergy
https://www.readbyqxmd.com/read/15786698/three-color-flowcytometric-analysis-of-mature-and-immature-hematological-malignancies-a-guideline-of-the-dutch-foundation-for-immunophenotyping-of-hematological-malignancies-sihon
#20
E R van Wering, E G van Lochem, M Leenders, A J van der Sluijs-Gelling, H Wind, J W Gratama, J Kraan, F W M B Preijers
Multiparameter flowcytometry offers an insight into differentiation pathways, maturation stages and abnormal features of cell (sub)populations thus helping to establish and classify hematological malignancies. The Dutch Foundation for Immunophenotyping of Hematological Malignancies (SIHON) has formulated a guideline for a rapid screening followed by confirmation and classification in a standardized way. For this aim seven carefully composed monoclonal antibody combinations are elucidated for screening the test sample in a first phase...
July 2004: Journal of Biological Regulators and Homeostatic Agents
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