keyword
MENU ▼
Read by QxMD icon Read
search

localization microscopy

keyword
https://www.readbyqxmd.com/read/29148780/a-spectroscopic-study-of-the-interaction-of-carboxyl-modified-gold-nanoparticles-with-liposomes-of-different-chain-lengths-and-controlled-drug-release-by-layer-by-layer-technology
#1
Nishu Kanwa, Soumya Kanti Dey, Chandan Adhikari, Anjan Chakraborty
In this manuscript, we investigate the interactions of carboxyl modified gold nanoparticles (AuC) with zwitterionic phospholipid liposomes of different chain lengths using a well-known membrane probe PRODAN by steady state and time resolved spectroscopy. We used three zwitterionic lipids, namely, dipalmitoylphosphatidylcholine (DPPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC); which are widely different in their phase transition temperatures to form liposome-AuC assemblies...
November 17, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29148536/characterization-of-foreign-materials-in-paraffin-embedded-pathological-specimens-using-in-situ-multi-elemental-imaging-with-laser-spectroscopy
#2
Benoit Busser, Samuel Moncayo, Florian Trichard, Vincent Bonneterre, Nicole Pinel, Frédéric Pelascini, Philippe Dugourd, Jean-Luc Coll, Michel D'Incan, Julie Charles, Vincent Motto-Ros, Lucie Sancey
Pathologists typically encounter many disparate exogenous materials in clinical specimens during their routine histopathological examinations, especially within the skin, lymph nodes, and lungs. These foreign substances may be free extracellular deposits or induce several clinical abnormalities or histopathological patterns. However, pathologists almost never investigate or report the chemical nature of exogenous metals in clinical specimens due to a lack of convenient and available technologies. In this paper, a novel strategy based on laser-induced breakdown spectroscopy (LIBS) technology is evaluated for in situ multi-elemental tissue imaging...
November 17, 2017: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/29146210/novel-intracellular-transport-refractory-mutations-in-kcnh2-identified-in-patients-with-symptomatic-long-qt-syndrome
#3
Daisuke Fukumoto, Wei-Guang Ding, Yuko Wada, Yusuke Fujii, Mari Ichikawa, Koichiro Takayama, Megumi Fukuyama, Koichi Kato, Hideki Itoh, Takeru Makiyama, Mariko Omatsu-Kanbe, Hiroshi Matsuura, Minoru Horie, Seiko Ohno
BACKGROUND: Missense mutations in KCNH2, a gene encoding the Kv11.1 channel, cause long QT syndrome (LQTS) type 2 primarily by disrupting the intracellular transport of Kv11.1 to the plasma membrane. The present study aimed to clarify the functional changes by two novel KCNH2 missense mutations. METHODS: We performed genetic screening of three unrelated symptomatic LQTS probands with family histories of cardiac symptoms. Chinese hamster ovary cells were transfected with wild-type (WT) and/or mutant KCNH2 plasmid and examined by patch-clamp technique...
November 13, 2017: Journal of Cardiology
https://www.readbyqxmd.com/read/29145645/waddlia-chondrophila-a-chlamydia-related-bacterium-has-a-negative-impact-on-human-spermatozoa
#4
D Baud, N Vulliemoz, A Ammerdorffer, J Gyger, G Greub, V Castella, M Stojanov
STUDY QUESTION: What is the impact of Waddlia chondrophila, an emerging Chlamydia-related bacterium associated with miscarriage, on human spermatozoa? SUMMARY ANSWER: W. chondrophila had a negative impact on human spermatozoa (decrease in viability and mitochondrial membrane potential) and was not entirely removed from infected samples by density gradient centrifugation. WHAT IS KNOWN ALREADY: Bacterial infection or colonization might have a deleterious effect on male fertility...
November 14, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29145401/anatomically-specific-intratubular-and-interstitial-biominerals-in-the-human-renal-medullo-papillary-complex
#5
Ling Chen, Ryan S Hsi, Feifei Yang, Benjamin A Sherer, Marshall L Stoller, Sunita P Ho
Limited information exists on the anatomically-specific early stage events leading to clinically detectable mineral aggregates in the renal papilla. In this study, quantitative multiscale correlative maps of structural, elemental and biochemical properties of whole medullo-papillary complexes from human kidneys were developed. Correlative maps of properties specific to the uriniferous and vascular tubules using high-resolution X-ray computed tomography, scanning and transmission electron microscopy, energy dispersive X-ray spectroscopy, and immunolocalization of noncollagenous proteins (NCPs) along with their association with anatomy specific biominerals were obtained...
2017: PloS One
https://www.readbyqxmd.com/read/29144609/internalization-kinetics-and-cytoplasmic-localization-of-functionalized-diatomite-nanoparticles-in-cancer-cells-by-raman-imaging
#6
Stefano Managò, Nunzia Migliaccio, Monica Terracciano, Michela Napolitano, Nicola M Martucci, Luca De Stefano, Ivo Rendina, Anna Chiara De Luca, Annalisa Lamberti, Ilaria Rea
Porous biosilica nanoparticles obtained from diatomites (DNPs) have been recently demonstrated to be non-toxic nanovectors of therapeutic agents in cancer cells. In this work, the internalization kinetics and intracellular spatial distribution of functionalized DNPs incubated with human lung epidermoid carcinoma cell line (H1355) up to 72 h are investigated by Raman imaging. The label-free Raman results are compared with confocal fluorescence microscopy and photoluminescence (PL) data. Raman bands specifically assigned to DNPs and cellular components provide evidence that the nanovectors are internalized and co-localize with lipid environments...
November 16, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/29143881/rmce-based-insect-cell-platform-to-produce-membrane-proteins-captured-on-hiv-1-gag-virus-like-particles
#7
João Vidigal, Bárbara Fernandes, Mafalda M Dias, Marco Patrone, António Roldão, Manuel J T Carrondo, Paula M Alves, Ana P Teixeira
Conformationally complex membrane proteins (MPs) are therapeutic targets in many diseases, but drug discovery has been slowed down by the lack of efficient production tools. Co-expression of MPs with matrix proteins from enveloped viruses is a promising approach to obtain correctly folded proteins at the surface of virus-like particles (VLPs), preserving their native lipidic environment. Here, we implemented a site-specific recombinase-mediated cassette exchange (RMCE) strategy to establish a reusable HIV-1 Gag-expressing insect cell line for fast production of target MPs on the surface of Gag-VLPs...
November 16, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29143694/cryo-scanning-electron-microscopy-sem-and-scanning-transmission-electron-microscopy-stem-in-sem-for-bio-and-organo-mineral-interface-characterization-in-the-environment
#8
Guillaume Wille, Jennifer Hellal, Patrick Ollivier, Annie Richard, Agnes Burel, Louis Jolly, Marc Crampon, Caroline Michel
Understanding biofilm interactions with surrounding substratum and pollutants/particles can benefit from the application of existing microscopy tools. Using the example of biofilm interactions with zero-valent iron nanoparticles (nZVI), this study aims to apply various approaches in biofilm preparation and labeling for fluorescent or electron microscopy and energy dispersive X-ray spectrometry (EDS) microanalysis for accurate observations. According to the targeted microscopy method, biofilms were sampled as flocs or attached biofilm, submitted to labeling using 4',6-diamidino-2-phenylindol, lectins PNA and ConA coupled to fluorescent dye or gold nanoparticles, and prepared for observation (fixation, cross-section, freezing, ultramicrotomy)...
November 16, 2017: Microscopy and Microanalysis
https://www.readbyqxmd.com/read/29143380/polo-like-kinase-1-inhibition-results-in-misaligned-chromosomes-and-aberrant-spindles-in-porcine-oocytes-during-the-first-meiotic-division
#9
Y Liao, D Lin, P Cui, B Abbasi, C Chen, Z Zhang, Y Zhang, Y Dong, R Rui, S Ju
Polo-like kinase 1 (Plk1), a type of serine/threonine protein kinase, has been implicated in various functions in the regulation of mitotic processes. However, these kinase's roles in meiotic division are not fully understood, particularly in the meiotic maturation of porcine oocytes. In this study, the expression and spatiotemporal localization of Plk1 were initially assessed in the meiotic process of pig oocytes by utilizing Western blotting with immunofluorescent staining combined with confocal microscopy imaging technique...
November 16, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29142323/nkx6-1-decline-accompanies-mitochondrial-dna-reduction-but-subtle-nucleoid-size-decrease-in-pancreatic-islet-%C3%AE-cells-of-diabetic-goto-kakizaki-rats
#10
Tomáš Špaček, Vojtěch Pavluch, Lukáš Alán, Nikola Capková, Hana Engstová, Andrea Dlasková, Zuzana Berková, František Saudek, Petr Ježek
Hypertrophic pancreatic islets (PI) of Goto Kakizaki (GK) diabetic rats contain a lower number of β-cells vs. non-diabetic Wistar rat PI. Remaining β-cells contain reduced mitochondrial (mt) DNA per nucleus (copy number), probably due to declining mtDNA replication machinery, decreased mt biogenesis or enhanced mitophagy. We confirmed mtDNA copy number decrease down to <30% in PI of one-year-old GK rats. Studying relations to mt nucleoids sizes, we employed 3D superresolution fluorescent photoactivable localization microscopy (FPALM) with lentivirally transduced Eos conjugate of mt single-stranded-DNA-binding protein (mtSSB) or transcription factor TFAM; or by 3D immunocytochemistry...
November 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29142280/mild-heat-induces-a-distinct-eustress-response-in-chinese-hamster-ovary-cells-but-does-not-induce-heat-shock-protein-synthesis
#11
Begüm Peksel, Imre Gombos, Mária Péter, László Vigh, Ádám Tiszlavicz, Mario Brameshuber, Gábor Balogh, Gerhard J Schütz, Ibolya Horváth, László Vigh, Zsolt Török
The current research on cellular heat stress management focuses on the roles of heat shock proteins (HSPs) and the proteostasis network under severe stress conditions. The mild, fever-type stress and the maintenance of membrane homeostasis are less well understood. Herein, we characterized the acute effect of mild, fever-range heat shock on membrane organization, and HSP synthesis and localization in two mammalian cell lines, to delineate the role of membranes in the sensing and adaptation to heat. A multidisciplinary approach combining ultrasensitive fluorescence microscopy and lipidomics revealed the molecular details of novel cellular "eustress", when cells adapt to mild heat by maintaining membrane homeostasis, activating lipid remodeling, and redistributing chaperone proteins...
November 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29142076/spatial-cues-and-not-spindle-pole-maturation-drive-the-asymmetry-of-astral-microtubules-between-new-and-pre-existing-spindle-poles
#12
Jette Lengefeld, Eric Yen, Xiuzhen Chen, Allen Leary, Jackie Vogel, Yves Barral
In many asymmetrically dividing cells, the microtubule-organizing centers (MTOCs; mammalian centrosome and yeast spindle pole body - SPB) nucleate more astral microtubules on one of the two spindle poles than the other. This differential activity generally correlates with the age of MTOCs and contributes to orienting the mitotic spindle within the cell. The asymmetry might results from the two MTOCs being in distinctive maturation states. We investigated this model in budding yeast. Using fluorophores with different maturation kinetics to label the outer plaque components of the SPB, we found that the Cnm67 protein is mobile, whereas Spc72 is not...
November 15, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/29142075/cone-myoid-elongation-involves-unidirectional-microtubule-movement-mediated-by-dynein-1
#13
Tylor R Lewis, Mariusz Zareba, Brian A Link, Joseph C Besharse
Teleosts and amphibians exhibit retinomotor movements, morphological changes in photoreceptors regulated by light and circadian rhythms. Cone myoid elongation occurs during dark adaptation leading to the positioning of the cone outer segment closer to the retinal pigment epithelium. While it has been shown that microtubules are essential for cone myoid elongation, the underlying mechanism has not been established. In this work, we generated a transgenic line of zebrafish expressing a photoconvertible form of α-tubulin (tdEOS-tubulin) specifically in cone photoreceptors...
November 15, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/29141422/coverage-dependent-molecular-assembly-of-anthraquinone-on-au-111
#14
Andrew S DeLoach, Brad R Conrad, T L Einstein, Daniel B Dougherty
A scanning tunneling microscopy study of anthraquinone (AQ) on the Au(111) surface shows that the molecules self-assemble into several structures depending on the local surface coverage. At high coverages, a close-packed saturated monolayer is observed, while at low coverages, mobile surface molecules coexist with stable chiral hexamer clusters. At intermediate coverages, a disordered 2D porous network interlinking close-packed islands is observed in contrast to the giant honeycomb networks observed for the same molecule on Cu(111)...
November 14, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/29141107/metal-transformation-as-a-strategy-for-bacterial-detoxification-of-heavy-metals
#15
Ashraf M M Essa, Mohamed A Al Abboud, Sayeed I Khatib
Microorganisms can modify the chemical and physical characters of metals leading to an alteration in their speciation, mobility, and toxicity. Aqueous heavy metals solutions (Hg, Cd, Pb, Ag, Cu, and Zn) were treated with the volatile metabolic products (VMPs) of Escherichia coli Z3 for 24 h using aerobic bioreactor. The effect of the metals treated with VMPs in comparison to the untreated metals on the growth of E. coli S1 and Staphylococcus aureus S2 (local isolates) was examined. Moreover, the toxic properties of the treated and untreated metals were monitored using minimum inhibitory concentration assay...
November 15, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/29140789/mechanism-and-dynamics-of-inpp5e-transport-into-and-inside-the-ciliary-compartment
#16
Stefanie Kristine Kösling, Eyad Kalawy Fansa, Stefano Maffini, Alfred Wittinghofer
The inositol polyphosphate 5´-phosphatase E (INPP5E) localizes to cilia. We showed that the carrier protein phosphodiesterase 6 delta subunit (PDE6δ) mediates the sorting of farnesylated INPP5E into cilia due to high affinity binding and release by Arl3 GTP. However, the dynamics of INPP5E transport into and inside the ciliary compartment are not fully understood. Here we first investigate the movement of INPP5E using live cell fluorescence microscopy and fluorescence recovery after photobleaching (FRAP) analysis...
August 28, 2017: Biological Chemistry
https://www.readbyqxmd.com/read/29139394/thickness-dependent-surface-plasmon-resonance-of-ito-nanoparticles-for-ito-in-sn-bilayer-structure
#17
Wenzuo Wei, Ruijin Hong, Ming Jing, Wen Shao, Chunxian Tao, Dawei Zhang
Tunablity of localized surface plasmon resonance (LSPR) in doped semiconductor nanoparticles (NPs), which represents an important characteristic in LSPR sensor applications, still remains a challenge. Here, indium tin oxide/indium tin alloy (ITO/In-Sn) bilayer films were deposited by electron beam evaporation and the properties, such as LSPR and surface morphology, were investigated by UV-vis-NIR double beam spectrophotometer and atomic force microscopy (AFM), respectively. By simply engineering the thickness of ITO/In-Sn NPs without any microstructure fabrications, LSPR wavelength of ITO NPs can be largely tuned from 858 to 1758 nm...
November 15, 2017: Nanotechnology
https://www.readbyqxmd.com/read/29138801/vitamin-d-receptor-activation-influences-the-erk-pathway-and-protects-against-neurological-deficits-and-neuronal-death
#18
Jie Yuan, Xin Guo, Zhengang Liu, Xiuqin Zhao, Yan Feng, Sixin Song, Changmeng Cui, Pei Jiang
Previous studies have demonstrated that global cerebral ischemia (GCI) causes neurological deficits and neuronal cell apoptosis. Calcitriol, a biologically active metabolite of vitamin D, exerts its endocrinological influence via nuclear vitamin D receptor. It is being assessed as an emerging therapeutic strategy in models of various medical conditions, including acute brain injury. The purpose of the present study was to investigate the neuroprotective effects of calcitriol on GCI and further refine the potential underlying mechanisms...
November 9, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29138789/preferential-s-se-occupation-in-an-anisotropic-res2-1-x-se2x-monolayer-alloy
#19
Wen Wen, Junhao Lin, Kazu Suenaga, Yuzheng Guo, Yiming Zhu, Hung-Pin Hsu, Liming Xie
Band structure engineering of two-dimensional (2D) metal dichalcogenides (TMDs) is crucial for their light-matter interaction and optoelectronic applications. Alloying of different metal or chalcogen elements with different stoichiometries in TMDs provides a versatile and efficient approach for modulating the electronic structure and properties of 2D materials. In 2D alloys, quantification of spatial distribution and local coordination of atoms facilitates the establishment of the structure-property relationship at the atomic scale...
November 15, 2017: Nanoscale
https://www.readbyqxmd.com/read/29137675/human-hyaluronic-acid-synthase-1-promotes-malignant-transformation-via-epithelial-to-mesenchymal-transition-micronucleation-and-centrosome-abnormalities
#20
Nguyet Nguyen, Awanit Kumar, Simi Chacko, Rodney J Ouellette, Anirban Ghosh
BACKGROUND: Human hyaluronic acid (HA) molecules are synthesized by three membrane spanning Hyaluronic Acid Synthases (HAS1, HAS2 and HAS3). Of the three, HAS1 is found to be localized more into the cytoplasmic space where it synthesizes intracellular HA. HA is a ubiquitous glycosaminoglycan, mainly present in the extracellular matrix (ECM) and on the cell surface, but are also detected intracellularly. Accumulation of HA in cancer cells, the cancer-surrounding stroma, and ECM is generally considered an independent prognostic factors for patients...
November 14, 2017: Cell Communication and Signaling: CCS
keyword
keyword
70552
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"