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Zhi Wang, Ravithree Senanayake, Christine M Aikens, Wen-Miao Chen, Chen-Ho Tung, Di Sun
Here we report the single-crystal structure, experimental and theoretical characterization of a 41-metal atom Au-Ag alloy nanocluster [Au3Ag38(SCH2Ph)24X5](2-) (1, X = Cl or Br). The nanocluster 1 is co-protected by thiolate and halogen atoms and features an all-metallic face-fused biicosahedral Au2@AuAg20 rod-like kernel enwrapped by the outermost Ag18(SCH2Ph)24X3 shell. Two sites on the surface of the biicosahedral kernel are partially occupied by Au and Ag atoms. The outer Ag18(SCH2Ph)24X3 shell is composed of two Ag6S6 cycles at the two poles and one Ag6S2X3 arc at the equator with both 2- and 3-coordinated Ag atoms, which has not been observed in gold or silver nanoclusters ever before...
October 17, 2016: Nanoscale
Seon Yeong Chae, Sun Young Park, Jin Oh Park, Kyu Jin Lee, Geuntae Park
This study reports a green approach for synthesis of gold nanoparticles using Gardenia jasminoides extract, and specifically, can potentially enhance anti senescence activity. Biological synthesis of gold nanoparticles is ecofriendly and effective for the development of environmentally sustainable nanoparticles compared with existing methods. Here, we developed a simple, fast, efficient, and ecofriendly approach to the synthesis of gold nanoparticles by means of a Gardenia jasminoides extract. These G. jasminoides extract-capped gold nanoparticles (GJ-GNPs) were characterized by UV-vis, high resolution transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), and Furrier transform infrared spectroscopy (FT-IR)...
September 28, 2016: Journal of Photochemistry and Photobiology. B, Biology
Salvatore Petta, Vincent Wai-Sun Wong, Calogero Cammà, Jean-Baptiste Hiriart, Grace Lai-Hung Wong, Fabio Marra, Julien Vergniol, Anthony Wing-Hung Chan, Vito Di Marco, Wassil Merrouche, Henry Lik-Yuen Chan, Marco Barbara, Brigitte Le-Bail, Umberto Arena, Antonio Craxì, Victor de Ledinghen
BACKGROUND AND AIMS: Liver stiffness measurement (LSM) frequently overestimates the severity of liver fibrosis In Nonalcoholic Fatty Liver Disease (NAFLD). Controlled Attenuation Parameter (CAP) is a new parameter provided by the same machine used for LSM, and associated with both steatosis and BMI, the two factors mostly affecting LSM peformance in NAFLD. We aimed to determine wheter prediction of liver fibrosis by LSM in NAFLD patients is affected by CAP values. METHODS: Patients (n=324) were assessed by clinical and histological (Kleiner score) features...
September 17, 2016: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
Xiaoming Sun, Qin Pan, Chunhui Yuan, Qilong Wang, Xiao-Lei Tang, Kan Ding, Xiang Zhou, Xiao-Lian Zhang
Because Mycobacterium bovis, termed bacillus Calmette-Guérin (BCG), the only available used tuberculosis (TB) vaccine, retains immunomodulatory properties that limit its protective immunogenicity, there are continuous efforts to identify the immunosuppression mechanism as well as new strategies for improving the immunogenicity of BCG. Here, an ssDNA aptamer "antibody" BM2 specifically bound to the mannose-capped lipoarabinomannan (ManLAM) of BCG was selected. BM2 significantly blocked ManLAM-mannose receptor (MR) binding, triggered ManLAM-CD44 signaling, and enhanced M1 macrophage and Th1 activation via cellular surface CD44 in vitro and in vivo...
September 14, 2016: Journal of the American Chemical Society
Rui Ye, Jun Liu, Zhiying Jia, Hongyang Wang, YongAn Wang, Wei Sun, Xuan Wu, Zhifei Zhao, Baolong Niu, Xingqi Li, Guanghai Dai, Jianxiong Li
BACKGROUND There is increasing evidence that adenosine triphosphate (ATP), a well-known neurotransmitter and neuromodulator in the central nervous system, plays an important role as an extracellular chemical messenger in the cochlea. MATERIAL AND METHODS Using a whole-cell recording technique, we studied the effects of ATP on isolated Hensen's cells, which are supporting cells in the cochlea, to determine if they are involved in the transduction of ions with hair cells. RESULTS ATP (0.1-10 µM) reduced the potassium current (IK+) in the majority of the recorded Hensen's cells (21 out of 25 cells)...
2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Ravikumar Aalinkeel, Bindukumar Nair, Chih-Kuang Chen, Supriya D Mahajan, Jessica L Reynolds, Hanguang Zhang, Haotian Sun, Donald E Sykes, Kailash C Chadha, Steven G Turowski, Katelyn D Bothwell, Mukund Seshadri, Chong Cheng, Stanley A Schwartz
Interleukin-8 (IL-8) is a pro-angiogenic cytokine associated with aggressive prostate cancer (CaP). We detected high levels of IL-8 in sera from patients with CaP compared with healthy controls and patients with benign prostatic hypertrophy. This study examines the role of IL-8 in the pathogenesis of metastatic prostate cancer. We developed a biocompatible, cationic polylactide (CPLA) nanocarrier to complex with and efficiently deliver IL-8 small interfering RNA (siRNA) to CaP cells in vitro and in vivo. CPLA IL-8 siRNA nanocomplexes (nanoplexes) protect siRNA from rapid degradation, are non-toxic, have a prolonged lifetime in circulation, and their net positive charge facilitates penetration of cell membranes and subsequent intracellular trafficking...
August 2016: Immunology
Xiangfeng Xi, Xiaobing Mo, Yan Xiao, Bo Yin, Chaochao Lv, Yuzhou Wang, Zhe Sun, Qingyuan Yang, Yali Yao, Yajie Xuan, Xiangdong Li, Y Adam Yuan, Kegong Tian
We report the strategies leading to the large-production of soluble non-tag full-length porcine circovirus type 2 (PCV2) Cap protein in Escherichia coli. Under neutral pH condition, the purified recombinant Cap protein derived from E. coli expression self-assembles into homogenous round virus-like particle at the similar size of that of the intact PCV2 virus, which is further characterized by Cryo-EM single particle structure determined at 4.5Å. The engineered PCV2 rCap VLP was tested as a subunit vaccine for the protective efficacy against PCV2 challenge on 3-week old piglets...
April 10, 2016: Journal of Biotechnology
Gregory D Kearney, Hui Hu, Xiaohui Xu, Marla B Hall, Jo Anne G Balanay
In hot weather, thermal heat generated by the body, combined with environmental heat from the sun, can lead outdoor workers to experience heat-related stress, severe illness, or even death. The aims of this study were to estimate the prevalence of heat-related symptoms and potential risk factors associated with sun safety-related behavior among Latino farmworkers. Data from interviewer-administered questionnaires were collected from a cross-sectional survey among farmworkers (N = 158) from August to September 2013...
2016: Journal of Agromedicine
Wen Chen, Wen Zeng, Jiansen Sun, Mingcan Yang, Li Li, Jingting Zhou, Yangxiao Wu, Jun Sun, Ge Liu, Rui Tang, Ju Tan, Chuhong Zhu
The application of tissue-engineered blood vessels (TEBVs) is the main developmental direction of vascular replacement therapy. Due to few and/or dysfunctional endothelial progenitor cells (EPCs), it is difficult to successfully construct EPC capture TEBVs in diabetes. RNA has a potential application in cell protection and diabetes treatment, but poor specificity and low efficiency of RNA transfection in vivo limit the application of RNA. On the basis of an acellular vascular matrix, we propose an aptamer-siRNA chimera-modified TEBV that can maintain a satisfactory patency in diabetes...
June 23, 2015: ACS Nano
Renzhong Li, Yunzhou Ruan, Qiang Sun, Xiexiu Wang, Mingting Chen, Hui Zhang, Yanlin Zhao, Jin Zhao, Cheng Chen, Caihong Xu, Wei Su, Yu Pang, Jun Cheng, Junying Chi, Qian Wang, Yunting Fu, Shitong Huan, Lixia Wang, Yu Wang, Daniel P Chin
BACKGROUND: China has a quarter of all patients with multidrug-resistant tuberculosis (MDRTB) worldwide, but less than 5% are in quality treatment programmes. In a before-and-after study we aimed to assess the effect of a comprehensive programme to provide universal access to diagnosis, treatment, and follow-up for MDRTB in four Chinese cities (population 18 million). METHODS: We designated city-level hospitals in each city to diagnose and treat MDRTB. All patients with smear-positive pulmonary tuberculosis diagnosed in Center for Disease Control (CDC) clinics and hospitals were tested for MDRTB with molecular and conventional drug susceptibility tests...
April 2015: Lancet Global Health
Chenxi Qian, Wei Sun, Liwei Wang, Changlong Chen, Kristine Liao, Wendong Wang, Jia Jia, Benjamin D Hatton, Gilberto Casillas, Marty Kurylowicz, Christopher M Yip, Melanie L Mastronardi, Geoffrey A Ozin
Here we describe for the first time the synthesis of colloidally stable, brightly luminescent perfluorodecyl-capped silicon nanocrystals and compare the properties of solutions and films made from them with those of their perhydrodecyl-capped relatives. The perfluorodecyl capping group compared to the perhydrodecyl capping group yields superior hydrophobicity and much greater resistance to air oxidation, the enhanced electron-withdrawing character induces blue shifts in the wavelength of photoluminescence, and the lower-frequency carbon-fluorine stretching modes disfavor non-radiative relaxation pathways and boost the absolute photoluminescence quantum yield...
November 12, 2014: Journal of the American Chemical Society
H P Borase, C D Patil, R B Salunkhe, R K Suryawanshi, B K Salunke, S V Patil
OBJECTIVE: To study the potential of phytolatex (latex of Jatropha gossypifolia) fabricated gold nanoparticles as promising candidate in sunscreen formulations for enhancement in sun protection factor. METHODS: In this study, plant latex was used as reducing and capping agent to synthesize gold nanoparticles. Latex fabricated gold nanoparticles were characterized by different analytical techniques such as UV-Vis spectroscopy, Fourier transforms infrared spectroscopy, dynamic light scattering, zeta potential, transmission electron microscopy and X-ray diffraction...
December 2014: International Journal of Cosmetic Science
Jing Sun, Yongwei Wei, Abdul Rauf, Yu Zhang, Yuanmei Ma, Xiaodong Zhang, Konstantin Shilo, Qingzhong Yu, Y M Saif, Xingmeng Lu, Lian Yu, Jianrong Li
UNLABELLED: Avian metapneumovirus (aMPV), also known as avian pneumovirus or turkey rhinotracheitis virus, is the causative agent of turkey rhinotracheitis and is associated with swollen head syndrome in chickens. Since its discovery in the 1970s, aMPV has been recognized as an economically important pathogen in the poultry industry worldwide. The conserved region VI (CR VI) of the large (L) polymerase proteins of paramyxoviruses catalyzes methyltransferase (MTase) activities that typically methylate viral mRNAs at guanine N-7 (G-N-7) and ribose 2'-O positions...
November 2014: Journal of Virology
Seydou Sylla, Yan-Long Cong, Yi-Xue Sun, Gui-Lian Yang, Xue-Mei Ding, Zhan-Qing Yang, Yu-Long Zhou, Minnan Yang, Chun-Feng Wang, Zhuang Ding
Post-weaning multisystemic wasting syndrome (PMWS) associated with porcine circovirus type 2 (PCV2) has caused the swine industry significant health challenges and economic damage. Although inactivated and subunit vaccines against PMWS have been used widely, so far no DNA vaccine is available. In this study, with the aim of exploring a new route for developing a vaccine against PCV2, the immunogenicity of a DNA vaccine was evaluated in mice. The pEGFP-N1 vector was used to construct a PCV2 Cap gene recombinant vaccine...
July 2014: Microbiology and Immunology
Eudes Euler de Souza Lucena, Danielle Clarisse Barbosa Costa, Éricka Janine Dantas da Silveira, Kenio Costa Lima
BACKGROUND: Workers from various occupational groups stand out as being more susceptible to photodamage. Behaviors that can reduce the risk of onset of these disorders include the limitation or reduction of sun exposure, use of photoprotective clothing, and correct use of sunscreen. The present study aimed to verify the use of photoprotection measures by beach workers on the lips and perioral regions, as well as investigate possible associations with sociodemographic, occupational, and health variables...
November 2014: International Journal of Dermatology
M Nardello, Paola Zuppella, V Polito, Alain Jody Corso, Sara Zuccon, M G Pelizzo
Future solar missions will investigate the Sun from very close distances and optical components are constantly exposed to low energy ions irradiation. In this work we present the results of a new experiment related to low energy alpha particles bombardments on Mo/Si multilayer optical coatings. Different multilayer samples, with and without a protecting capping layer, have been exposed to low energy alpha particles (4keV), fixing the ions fluency and varying the time of exposure in order to change the total dose accumulated...
November 18, 2013: Optics Express
Andrei Maiseyeu, Hui-Yu Yang, Gajalakshmi Ramanathan, Fen Yin, Robert L Bard, Masako Morishita, J Timothy Dvonch, Lu Wang, Catherine Spino, Bhramar Mukherjee, Marcus A Badgeley, Alma Barajas-Espinosa, Qinghua Sun, Jack Harkema, Sanjay Rajagopalan, Jesus A Araujo, Robert D Brook
CONTEXT: High-density lipoprotein (HDL) particles perform numerous vascular-protective functions. Animal studies demonstrate that exposure to fine or ultrafine particulate matter (PM) can promote HDL dysfunction. However, the impact of PM on humans remains unknown. OBJECTIVE: We aimed to determine the effect of exposure to coarse concentrated ambient particles (CAP) on several metrics of HDL function in healthy humans. METHODS: Thirty-two adults (25...
January 2014: Inhalation Toxicology
Hemant P Borase, Chandrashekhar D Patil, Rahul K Suryawanshi, Satish V Patil
The present work provides scientific support on the use of latex of Ficus carica to synthesize stable silver nanoparticles (AgNPs). AgNPs synthesized immediately after the addition of latex to silver nitrate solution at room temperature. Synthesized nanoparticles were of spherical shape with average size of 163.7 nm. Fourier transform infrared spectroscopy analysis revealed capping of proteins and phenolic compound on AgNPs, while X-ray diffraction analysis confirmed the fcc nature of AgNPs. Particles formed were stable for a long time (6 months)...
October 2013: Applied Biochemistry and Biotechnology
Jong Soo Lee, Hoon Jai Chun, Jae Min Lee, Young Jae Hwang, Seung Han Kim, Eun Sun Kim, Yoon Tae Jeen, Hyun Joo Lee
A sharp, impacted fish bone in the esophagus is an indication for urgent endoscopy. Endoscopic removal of such an object is a challenging task. An endoscopic protector hood is then used to remove the object. However, an endoscopic hood protector is not always available. In a patient with a large hiatal hernia, the protector hood may not return to the original shape when it passes through the gastroesophageal junction and therefore may not properly protect the esophageal mucosa from the sharp foreign body. In our case, it was impossible to deploy the endoscopic hood protector through the gastroesophageal junction despite multiple attempts...
April 2013: Korean Journal of Gastroenterology, Taehan Sohwagi Hakhoe Chi
Eudes Euler de Souza Lucena, Danielle Clarisse Barbosa Costa, Éricka Janine Dantas da Silveira, Kenio Costa de Lima
OBJECTIVE: To assess the prevalence and factors associated with orolabial lesions caused by sun exposure in beach workers. METHODS: Cross-sectional study with 362 beach workers from five urban beaches in the city of Natal, northeastern Brazil, from August to December 2010. All subjects completed a validated questionnaire to collect personal, occupational, and health-related information and underwent an orolabial clinical examination by trained examiners. Potential associations between sociodemographic, occupational, and health-related variables and the presence of orolabial lesions were assessed using the chi-square test at a 5% significance level...
December 2012: Revista de Saúde Pública
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