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https://www.readbyqxmd.com/read/29744447/in-vivo-evaluation-of-urokinase-loaded-hollow-nanogels-for-sonothrombolysis-on-suture-embolization-induced-acute-ischemic-stroke-rat-model
#1
Yuming Teng, Haiqiang Jin, Ding Nan, Mengnan Li, Chenghe Fan, Yuanyuan Liu, Pu Lv, Wei Cui, Yongan Sun, Hongjun Hao, Xiaozhong Qu, Zhenzhong Yang, Yining Huang
The urokinase-type plasminogen activator (uPA) loaded hollow nanogels (nUK) were synthesized by a one-step reaction of glycol chitosan and aldehyde capped poly (ethylene oxide). The resultant formulation is sensitive to diagnostic ultrasound (US) of 2 MHz. Herein, we evaluated the in vivo sonothrombolysis performance of the nUK on acute ischemic stroke rat model which was established by suture embolization of middle cerebral artery (MCA). Via intravenous (i.v.) administration, the experimental data prove a controlled release of the therapeutic protein around the clots under ultrasound stimulation, leading to enhanced thrombolysis efficiency of the nUK, evidenced from smaller infarct volume and better clinical scores when compared to the i...
March 2018: Bioactive Materials
https://www.readbyqxmd.com/read/29704741/stepwise-acid-active-organic-inorganic-hybrid-drug-delivery-system-for-cancer-therapy
#2
Hui Chen, Zhongyin Chen, Ying Kuang, Shuang Li, Min Zhang, Jia Liu, Zhengguang Sun, Bingbing Jiang, Xueqin Chen, Cao Li
Due to the difference of pH values between normal tissues, tumor tissues and intracellular environments, DOX@MSN-CD-PEG, a stepwise-acid-active organic/inorganic hybrid drug delivery system (DDS) was reported in this article. The inorganic mesoporous silica nanoparticle (MSN) was introduced for loading of doxorubicin hydrochloride (DOX). Then organic components were applied to achieve the stepwise-acid-active intracellular drug release: MSN was capped with a β-cyclodextrine (β-CD) based host-guest system via pH-sensitive epoxy bond...
April 21, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29692031/-exogenous-no-application-effectively-alleviates-the-allelochemical-stress-on-cucumber-root-border-cells-caused-by-eupatorium-adenophorum-extracts
#3
Xiao Huan Yang, Wen Xiu Yang, Liang Liang Sun, Qian Zhao, Yong Heng Cao, Jin Hu Ma
Using suspension cultures of cucumber (Cucumis sativus) cultivar Jinyou 35, we investigated the effects of allelochemical stresses induced by Eupatorium adenophorum extracts on root border cells (RBC), and the role of exogenous NO application in alleviation of the damage of root tips exposed to E. adenophorum extracts. The results showed that, 1000 mg·L-1 E. adenophorum extracts had significant damage to the cucumber root tip, resulting in severe tissue damage, exfoliated surface cells and irregular arrangement of inner cells, while those damages could be effectively alleviated by spraying exogenous NO...
January 2018: Ying Yong Sheng Tai Xue Bao, the Journal of Applied Ecology
https://www.readbyqxmd.com/read/29682802/facial-exposure-to-ultraviolet-radiation-predicted-sun-protection-effectiveness-of-various-hat-styles
#4
C Backes, A Religi, L Moccozet, L Vuilleumier, D Vernez, J-L Bulliard
BACKGROUND/PURPOSE: Solar ultraviolet radiation (UVR) doses received by individuals are highly influenced by behavioural and environmental factors. This study aimed at quantifying hats' sun protection effectiveness in various exposure conditions, by predicting UVR exposure doses and their anatomical distributions. METHODS: A well-defined three-dimensional head morphology and four hat styles (a cap, a helmet, a middle- and a wide-brimmed hat) were added to a previously published model...
April 23, 2018: Photodermatology, Photoimmunology & Photomedicine
https://www.readbyqxmd.com/read/29490687/genetic-ablation-of-dynactin-p150-glued-in-postnatal-neurons-causes-preferential-degeneration-of-spinal-motor-neurons-in-aged-mice
#5
Jia Yu, Chen Lai, Hoon Shim, Chengsong Xie, Lixin Sun, Cai-Xia Long, Jinhui Ding, Yan Li, Huaibin Cai
BACKGROUND: Dynactin p150Glued , the largest subunit of the dynactin macromolecular complex, binds to both microtubules and tubulin dimers through the N-terminal cytoskeleton-associated protein and glycine-rich (CAP-Gly) and basic domains, and serves as an anti-catastrophe factor in stabilizing microtubules in neurons. P150Glued also initiates dynein-mediated axonal retrograde transport. Multiple missense mutations at the CAP-Gly domain of p150Glued are associated with motor neuron diseases and other neurodegenerative disorders, further supporting the importance of microtubule domains (MTBDs) in p150Glued functions...
March 1, 2018: Molecular Neurodegeneration
https://www.readbyqxmd.com/read/29424135/-generation-and-characterization-of-recombinant-classical-swine-fever-virus-c-strain-expressing-the-cap-protein-of-porcine-circovirus-type-2
#6
Lingkai Zhang, Yongfeng Li, Libao Xie, Yuan Sun, Xiao Wang, Hua-Ji Qiu
Classical swine fever (CSF), caused by classical swine fever virus (CSFV), is a devastating viral disease in swine, leading to significant economic losses to the pig husbandry. C-strain is one of the best modified live vaccines against CSF. The vaccine is highly safe and efficacious and can provide rapid and complete protection against essentially all genotypes of CSFV. Co-infections of pigs with CSFV and porcine circovirus type 2 (PCV2) occur frequently in the field, making it difficult to control the associated diseases...
February 25, 2018: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/29205568/johnson-solids-anion-templated-silver-thiolate-clusters-capped-by-sulfonate
#7
Zhi Wang, Hai-Feng Su, Xing-Po Wang, Quan-Qin Zhao, Chen-Ho Tung, Di Sun, Lan-Sun Zheng
Sulfonates were incorporated into six novel high-nuclearity silver(I) thiolate clusters under the guidance of anion templates varied from S2- , SO4 2- , α-[Mo5 O18 ]6- , β-[Mo5 O18 ]6- , [Mo2 O8 ]4- , to [Mo4 O14 (SO4 )]6- . Single crystal X-ray analysis revealed that SD/Ag1, SD/Ag3, SD/Ag5, and SD/Ag6 are discrete [S@Ag60 ], [α-Mo5 O18 @Ag36 ], [Mo2 O8 @Ag30 ]2 , and [Mo4 O14 (SO4 )@Ag73 ] clusters, respectively, whereas SD/Ag2 and SD/Ag4 are one-dimensional (1D) chains based on the [SO4 @Ag20 ] and [β-Mo5 O18 @Ag36 ] cluster subunits, respectively...
February 1, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29189898/immunogenicity-of-recombinant-vaccinia-virus-vaccines-co-expressing-gp3-gp5-of-european-prrsv-and-cap-protein-of-pcv2-in-pigs
#8
Jicheng Han, Haibin Ma, Liang Cao, Jie Jing, Pengpeng Xiao, Wenchao Sun, Changzhan Xie, Shubo Wen, Yiquan Li, Mingyao Tian, Huijun Lu, Ningyi Jin
Porcine reproductive and respiratory syndrome (PRRS) is almost always caused by the North American strain of PRRS virus (PRRSV) in China; the European genotype of PRRSV has emerged in China. The mixed infection of PRRSV and Porcine circovirus type 2 virus (PCV2) are always found in pigs and PRRSV-augmented PCV2 replication and serious clinical symptoms. Current vaccines cannot protect mixed European PRRSV and PCV2 infections. Therefore, the development of a safe and effective new vaccine to prevent and control the mixed infection of European PRRSV and PCV2 is both urgent and necessary...
February 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29160296/structure-of-the-fission-yeast-s-pombe-telomeric-tpz1-poz1-rap1-complex
#9
Jing Xue, Hongwen Chen, Jian Wu, Miho Takeuchi, Haruna Inoue, Yanmei Liu, Hong Sun, Yong Chen, Junko Kanoh, Ming Lei
Telomeric shelterin complex caps chromosome ends and plays a crucial role in telomere maintenance and protection. In the fission yeast Schizosaccharomyces pombe, shelterin is composed of telomeric single- and double-stranded DNA-binding protein subcomplexes Pot1-Tpz1 and Taz1-Rap1, which are bridged by their interacting protein Poz1. However, the structure of Poz1 and how Poz1 functions as an interaction hub in the shelterin complex remain unclear. Here we report the crystal structure of Poz1 in complex with Poz1-binding motifs of Tpz1 and Rap1...
December 2017: Cell Research
https://www.readbyqxmd.com/read/29039911/halide-re-shelled-quantum-dot-inks-for-infrared-photovoltaics
#10
James Z Fan, Mengxia Liu, Oleksandr Voznyy, Bin Sun, Larissa Levina, Rafael Quintero-Bermudez, Min Liu, Olivier Ouellette, F Pelayo García de Arquer, Sjoerd Hoogland, Edward H Sargent
Colloidal quantum dots are promising materials for tandem solar cells that complement silicon and perovskites. These devices are fabricated from solution phase; however, existing methods for making infrared-bandgap CQD inks suffer agglomeration and fusion during solution exchange. Here we develop a ligand exchange that provides robust surface protection and thereby avoids aggregation. First, we exchanged long oleic acid ligands to a mixed system comprising medium-chain ammonium and anionic chloride ligands; we then reshelled the surface using short halides and pseudohalide ligands that enabled transfer to a polar solvent...
October 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28993266/a-protective-umbrella-nanoplatform-for-loading-icg-and-multi-modal-imaging-guided-phototherapy
#11
Jie Zhou, Lingchang Meng, Chong Sun, Weiran Ye, Chengqun Chen, Bin Du
In order to prevent the aggregation of ICG and enhance its stability, a novel nanoplatform (TiO2 :Yb,Ho,F-β-CD@ICG/HA) was designed for NIR-induced phototherapy along with multi-mode imaging(UCL/MRI/Flu). In this nanosysytem: TiO2 :Yb,Ho,F was used as upconversion materials and applied in vivo for the first time; β-CD acted as a "protective umbrella" to load separated ICG and avoid the low phototherapy efficiency because of its aggregation; HA was the capping agent of β-CD to prevent ICG unexpected leaking and a target to recognize CD44 receptor...
February 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28940759/the-bottle-gourd-genome-provides-insights-into-cucurbitaceae-evolution-and-facilitates-mapping-of-a-papaya-ring-spot-virus-resistance-locus
#12
Shan Wu, Md Shamimuzzaman, Honghe Sun, Jerome Salse, Xuelian Sui, Alan Wilder, Zujian Wu, Amnon Levi, Yong Xu, Kai-Shu Ling, Zhangjun Fei
Bottle gourd (Lagenaria siceraria) is an important vegetable crop as well as a rootstock for other cucurbit crops. In this study, we report a high-quality 313.4-Mb genome sequence of a bottle gourd inbred line, USVL1VR-Ls, with a scaffold N50 of 8.7 Mb and the longest of 19.0 Mb. About 98.3% of the assembled scaffolds are anchored to the 11 pseudomolecules. Our comparative genomic analysis identifies chromosome-level syntenic relationships between bottle gourd and other cucurbits, as well as lineage-specific gene family expansions in bottle gourd...
September 23, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28892364/bulky-surface-ligands-promote-surface-reactivities-of-ag141x12-s-adm-40-3-x-cl-br-i-nanoclusters-models-for-multiple-twinned-nanoparticles
#13
Liting Ren, Peng Yuan, Haifeng Su, Sami Malola, Shuichao Lin, Zichao Tang, Boon K Teo, Hannu Häkkinen, Lansun Zheng, Nanfeng Zheng
Surface ligands play important roles in controlling the size and shape of metal nanoparticles and their surface properties. In this work, we demonstrate that the use of bulky thiolate ligands, along with halides, as the surface capping agent promotes the formation of plasmonic multiple-twinned Ag nanoparticles with high surface reactivities. The title nanocluster [Ag141X12(S-Adm)40](3+) (where X = Cl, Br, I; S-Adm = 1-adamantanethiolate) has a multiple-shell structure with an Ag71 core protected by a shell of Ag70X12(S-Adm)40...
September 18, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28872849/thiol-induced-synthesis-of-phosphine-protected-gold-nanoclusters-with-atomic-precision-and-controlling-the-structure-by-ligand-metal-engineering
#14
Shan Jin, Wenjun Du, Shuxin Wang, Xi Kang, Man Chen, Daqiao Hu, Shuang Chen, Xuejuan Zou, Guodong Sun, Manzhou Zhu
Efficient synthesis of atomically precise phosphine-capped gold nanocluster (with >10 metal atoms) is important to deeply understand the relationship between structure and properties. Herein, we successfully utilize the thiol-induced synthesis method and obtain three atomically precise phosphine-protected gold nanoclusters. Single-crystal X-ray structural analysis reveals that the nanoclusters are formulated as [Au13 (Dppm)6 ](BPh4 )3 , [Au18 (Dppm)6 Br4 ](BPh4 )2 , and [Au20 (Dppm)6 (CN)6 ] (where Dppm stands for bis(diphenylphosphino)methane), which are further confirmed by electrospray ionization mass spectrometry, thermogravimetric analysis, and X-ray photoelectron spectroscopy...
September 18, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28790819/kalopanacis-cortex-extract-capped-gold-nanoparticles-activate-nrf2-signaling-and-ameliorate-damage-in-human-neuronal-sh-sy5y-cells-exposed-to-oxygen-glucose-deprivation-and-reoxygenation
#15
Sun Young Park, Seon Yeong Chae, Jin Oh Park, Kyu Jin Lee, Geuntae Park
Recently, environment-friendly synthesis of gold nanoparticles (GNPs) has been extensively explored by biologists and chemists. However, significant research is still required to determine whether "eco-friendly" GNPs are beneficial to human health and to elucidate the molecular mechanisms of their effects on human cells. We used human neuronal SH-SY5Y cells to show that treatment with Kalopanacis Cortex extract-capped GNPs (KC-GNs), prepared via an eco-friendly, fast, one-pot synthetic route, protected neuronal cells against oxygen-glucose deprivation/reoxygenation (OGD/R)-induced damage...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28769561/nanoparticle-orientationally-displayed-antigen-epitopes-improve-neutralizing-antibody-level-in-a-model-of-porcine-circovirus-type-2
#16
Peiyang Ding, Teng Zhang, Yafei Li, Man Teng, Yaning Sun, Xiao Liu, Shujun Chai, Enmin Zhou, Qianyue Jin, Gaiping Zhang
Recent advancements in biotechnology have enabled the rapid identification and subsequent expression of pathogenic microbial major antigens that induce protective immune responses. However, subunit vaccines have not been successfully commercialized mainly due to the lack of sufficient levels of neutralizing antibodies (NAs). High levels of NA rely on the efficient recognition and cross-linking of multiple neutralizing epitopes with B-cell receptors (BCRs). Nanoparticles are able to display coupled antigenic arrays at high density and provide multiple binding molecular scenarios with BCRs...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28608526/clothing-protection-from-ultraviolet-radiation-a-new-method-for-assessment
#17
Ryan Gage, William Leung, James Stanley, Anthony Reeder, Michelle Barr, Tim Chambers, Moira Smith, Louise Signal
Clothing modifies ultraviolet radiation (UVR) exposure from the sun and has an impact on skin cancer risk and the endogenous synthesis of vitamin D. There is no standardized method available for assessing body surface area (BSA) covered by clothing, which limits generalizability between study findings. We calculated the body cover provided by 38 clothing items using diagrams of BSA, adjusting the values to account for differences in BSA by age. Diagrams displaying each clothing item were developed and incorporated into a coverage assessment procedure (CAP)...
November 2017: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/28497612/accurate-assessment-of-alpha-gal-syndrome-using-cetuximab-and-bovine-thyroglobulin-specific-ige
#18
Da Woon Sim, Jong Sun Lee, Kyung Hee Park, Kyoung Yong Jeong, Young-Min Ye, Jae-Hyun Lee, Jung-Won Park
SCOPE: IgE against galactose-α-1,3-galactose (α-Gal) causes alpha-gal syndrome. Bovine thyroglobulin (BTG) and cetuximab share this epitope. We aimed to determine the utility of specific IgE (sIgE) against cetuximab as compared to BTG for diagnosing alpha-gal syndrome. METHODS AND RESULTS: Twelve patients with alpha-gal syndrome, 11 patients with immediate beef or pork allergy, 18 asymptomatic individuals with meat sensitization, and 10 non-atopic subjects were enrolled...
May 12, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28247882/anion-templated-nanosized-silver-clusters-protected-by-mixed-thiolate-and-diphosphine
#19
Xiao-Yu Li, Zhi Wang, Hai-Feng Su, Sheng Feng, Mohamedally Kurmoo, Chen-Ho Tung, Di Sun, Lan-Sun Zheng
S(2-), MoO4(2-), and Mo6O22(8-) were successfully employed as templates in the formation of six high-symmetry polynuclear silver clusters as exemplified in the single-crystal X-ray structures of [S@Ag18((t)BuC6H4S)16(dppp)4]·DMF·5CH3CN·3CH3OH (1), [MoO4@Ag24(MeC6H4S)12(dppm)6(MoO4)4]·2BF4·C2H5NO (2), [MoO4@Ag24(MeC6H4S)12(dppm)6(MoO4)4]·2CF3SO3 (3), [MoO4@Ag24(MeC6H4S)12(dppf)6(MoO4)4]·2CF3SO3 (4), [MoO4@Ag24(MeC6H4S)12(dppb)6(MoO4)4]·2CF3SO3 (5) and [Mo6O22@Ag46((t)BuC6H4S)32(dppm)4(CH3CN)8]·6CF3SO3 (6) (dppp = 1,3-bis(diphenylphosphino)propane, dppm = bis(diphenylphosphino)methane, dppf = 1,1'-bis(diphenylphosphino)ferrocene and dppb = 1,4-bis(diphenylphosphino)butane)...
March 1, 2017: Nanoscale
https://www.readbyqxmd.com/read/28218285/super-hydrophobic-silver-doped-tio2-polycarbonate-coatings-created-on-various-material-substrates-with-visible-light-photocatalysis-for-self-cleaning-contaminant-degradation
#20
Zhengjian Li, Zongzhao Sun, Zhiqiang Duan, Rui Li, Yanli Yang, Jingyi Wang, Xiaoxia Lv, Wei Qi, Hua Wang
In the present work, a facile and efficient fabrication method has been developed for creating super-hydrophobic coatings of silver-doped TiO2@polycarbonate (TiO2 (Ag)@PC) on the substrates of different materials with photocatalytic self-cleaning performances simply by the "dipping and drying" process. The substrates were first patterned with glue and then deposited with the dopamine-capped TiO2 (Ag)@PC (DA-TiO2 (Ag)@PC) nanocomposites, followed by the further etching with dimethylbenzene. The so prepared super-hydrophobic E-DA-TiO2(Ag)@PC coatings could present the lotus leaf-like porous architectures, high adhesion stability, and especially the visible-light photocatalysis for organic contaminant degradation, thus promising the wide outdoor and indoor applications like water proofing, metal erosion protection, and surface self-cleaning...
February 20, 2017: Scientific Reports
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