keyword
MENU ▼
Read by QxMD icon Read
search

human factors engineering

keyword
https://www.readbyqxmd.com/read/29667149/three-dimensional-reconstructed-eccrine-sweat-glands-with-vascularization-and-cholinergic-and-adrenergic-innervation
#1
Mingjun Zhang, Haihong Li, Liyun Chen, Shuhua Fang, Sitian Xie, Changmin Lin
Functional integrity of the regenerated tissues requires not only structural integrity but also vascularization and innervation. We previously demonstrated that the three-dimensional (3D) reconstructed eccrine sweat glands had similar structures as those of the native ones did, but whether the 3D reconstructed glands possessing vascularization and innervation was still unknown. In the study, Matrigel-embedded eccrine sweat gland cells were implanted under the inguinal skin. Ten weeks post-implantation, the vascularization, and innervation in the 10-week reconstructed eccrine sweat glands and native human eccrine sweat glands were detected by immunofluorescence staining...
April 17, 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29666172/genetic-editing-of-colonic-organoids-provides-a-molecularly-distinct-and-orthotopic-preclinical-model-of-serrated-carcinogenesis
#2
Tamsin R M Lannagan, Young K Lee, Tongtong Wang, Jatin Roper, Mark L Bettington, Lochlan Fennell, Laura Vrbanac, Lisa Jonavicius, Roshini Somashekar, Krystyna Gieniec, Miao Yang, Jia Q Ng, Nobumi Suzuki, Mari Ichinose, Josephine A Wright, Hiroki Kobayashi, Tracey L Putoczki, Yoku Hayakawa, Simon J Leedham, Helen E Abud, Ömer H Yilmaz, Julie Marker, Sonja Klebe, Pratyaksha Wirapati, Siddhartha Mukherjee, Sabine Tejpar, Barbara A Leggett, Vicki L J Whitehall, Daniel L Worthley, Susan L Woods
OBJECTIVE: Serrated colorectal cancer (CRC) accounts for approximately 25% of cases and includes tumours that are among the most treatment resistant and with worst outcomes. This CRC subtype is associated with activating mutations in the mitogen-activated kinase pathway gene, BRAF , and epigenetic modifications termed the CpG Island Methylator Phenotype, leading to epigenetic silencing of key tumour suppressor genes. It is still not clear which (epi-)genetic changes are most important in neoplastic progression and we begin to address this knowledge gap herein...
April 17, 2018: Gut
https://www.readbyqxmd.com/read/29664683/examining-cybersecurity-of-cyberphysical-systems-for-critical-infrastructures-through-work-domain-analysis
#3
Hao Wang, Nathan Lau, Ryan M Gerdes
OBJECTIVE: The aim of this study was to apply work domain analysis for cybersecurity assessment and design of supervisory control and data acquisition (SCADA) systems. BACKGROUND: Adoption of information and communication technology in cyberphysical systems (CPSs) for critical infrastructures enables automated and distributed control but introduces cybersecurity risk. Many CPSs employ SCADA industrial control systems that have become the target of cyberattacks, which inflict physical damage without use of force...
April 1, 2018: Human Factors
https://www.readbyqxmd.com/read/29663920/advances-in-the-engineering-of-the-gene-editing-enzymes-and-the-genomes-understanding-and-handling-the-off-target-effects-of-crispr-cas9
#4
Yufang Yin, Qian Wang, Li Xiao, Fengjiao Wang, Zhuo Song, Cuilan Zhou, Xuan Liu, Chungen Xing, Nongyue He, Kai Li, Yan Feng, Jia Zhang
In the past decades, significant progresses have been achieved in genetic engineering of nucleases. Among the genetically engineered nucleases, zinc finger nucleases, transcription activator-like (TAL) effector nucleases, and CRIPSPR/Cas9 system form a new field of gene editing. The gene editing efficiency or targeting effect and the off-target effect are the two major determinant factors in evaluating the usefulness of a new enzyme. Engineering strategies in improving these gene editing enzymes, particularly in minimizing their off-target effects, are the focus of this paper...
March 1, 2018: Journal of Biomedical Nanotechnology
https://www.readbyqxmd.com/read/29656478/transplantation-of-neural-progenitor-cells-expressing-glial-cell-line-derived-neurotrophic-factor-into-the-motor-cortex-as-a-strategy-to-treat-amyotrophic-lateral-sclerosis
#5
Gretchen M Thomsen, Pablo Avalos, Annie A Ma, Mor Alkaslasi, Noell Cho, Livia Wyss, Jean-Philippe Vit, Marlesa Godoy, Patrick Suezaki, Oksana Shelest, Krystof S Bankiewicz, Clive N Svendsen
Early dysfunction of cortical motor neurons may underlie the initiation of amyotrophic lateral sclerosis (ALS). As such, the cortex represents a critical area of ALS research and a promising therapeutic target. In the current study, human cortical-derived neural progenitor cells engineered to secrete glial cell line-derived neurotrophic factor (GDNF) were transplanted into the SOD1G93A ALS rat cortex, where they migrated, matured into astrocytes, and released GDNF. This protected motor neurons, delayed disease pathology and extended survival of the animals...
April 15, 2018: Stem Cells
https://www.readbyqxmd.com/read/29651417/aging-mouse-models-reveal-complex-tumor-microenvironment-interactions-in-cancer-progression
#6
REVIEW
Hidetoshi Mori, Robert D Cardiff, Alexander D Borowsky
Mouse models and genetically engineered mouse models (GEMM) are essential experimental tools for the understanding molecular mechanisms within complex biological systems. GEMM are especially useful for inferencing phenocopy information to genetic human diseases such as breast cancer. Human breast cancer modeling in mice most commonly employs mammary epithelial-specific promoters to investigate gene function(s) and, in particular, putative oncogenes. Models are specifically useful in the mammary epithelial cell in the context of the complete mammary gland environment...
2018: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/29649529/mesoporous-silicate-nanoparticles-3d-nanofibrous-scaffold-mediated-dual-drug-delivery-for-bone-tissue-engineering
#7
Qingqing Yao, Yangxi Liu, Balaranjan Selvaratnam, Ranjit T Koodali, Hongli Sun
Controlled delivery systems play a critical role in the success of bone morphogenetic proteins (i.e., BMP2 and BMP7) for challenged bone repair. Instead of single-drug release that is currently and commonly prevalent, dual-drug delivery strategies are highly desired to achieve effective bone regeneration because natural bone repair process is driven by multiple factors. Particularly, angiogenesis is essential for osteogenesis and requires more than just one factor (e.g., Vascular Endothelial Growth Factor, VEGF)...
April 9, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29649411/the-in-vitro-differentiation-of-gdnf-gene-engineered-amniotic-fluid-derived-stem-cells-into-renal-tubular-epithelial-like-cells
#8
Ying Lu, Zhuojun Wang, Lu Chen, Jia Wang, Shulin Li, Caixia Liu, Dong Sun
Amniotic fluid is an alternative source of stem cells, and human amniotic fluid-derived stem cells (AFSCs) obtained from a small amount of amniotic fluid collected during the second trimester represent a novel source for use in regenerative medicine. These AFSCs are characterized by lower diversity, a higher proliferation rate and a wider differentiation capability than adult mesenchymal stem cells (MSCs). AFSCs are selected based on the cell surface marker c-kit receptor (CD117) using immunomagnetic sorting...
March 15, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29643181/smooth-muscle-cells-differentiated-from-human-mesenchymal-stem-cells-regulated-by-microrna-mir-503-and-mir-222-5p-are-suitable-for-vascular-tissue-engineering
#9
Wenduo Gu, Xuechong Hong, Alexandra Le Bras, Witold N Nowak, Shirin Issa Bhaloo, Jiacheng Deng, Yao Xie, Yanhua Hu, Xiong Z Ruan, Qingbo Xu
Tissue-engineered vascular grafts with long-term patency are greatly needed in the clinical settings, and smooth muscle cells (SMCs) are a critical graft component. Human mesenchymal stem cells (MSCs) are used for generating SMCs and understanding the underlying regulatory mechanisms of the MSC-to-SMC differentiation process could improve SMC generation in the clinic. Here, we found that in response to stimulation of transforming growth factor-β 1 (TGFβ1), human umbilical cord-derived MSCs abundantly express the SMC markers α-smooth muscle actin (αSMA), smooth muscle protein 22 (SM22), calponin and smooth muscle myosin heavy chain (SMMHC) at both gene and protein levels...
April 11, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29629346/lycopene-protects-against-spontaneous-ovarian-cancer-formation-in-laying-hens
#10
Kazim Sahin, Engin Yenice, Mehmet Tuzcu, Cemal Orhan, Cengizhan Mizrak, Ibrahim H Ozercan, Nurhan Sahin, Bahiddin Yilmaz, Birdal Bilir, Bulent Ozpolat, Omer Kucuk
Background: Dietary intake of lycopene has been associated with a reduced risk of ovarian cancer, suggesting its chemopreventive potential against ovarian carcinogenesis. Lycopene's molecular mechanisms of action in ovarian cancer have not been fully understood. Therefore, in the present study, we investigated the effects of lycopene on the ovarian cancer formation using the laying hen model, a biologically relevant animal model of spontaneous ovarian carcinogenesis due to high incidence rates similar to humans...
March 2018: Journal of Cancer Prevention
https://www.readbyqxmd.com/read/29626696/fine-tuning-pro-angiogenic-effects-of-cobalt-for-simultaneous-enhancement-of-vascular-endothelial-growth-factor-secretion-and-implant-neovascularization
#11
Yoke Chin Chai, Luis Freitas Mendes, Nick van Gastel, Geert Carmeliet, Frank P Luyten
Rapid neovascularization of a tissue-engineered (TE) construct by the host vasculature is quintessential to warrant effective bone regeneration. This process can be promoted through active induction of angiogenic growth factor secretion or by implementation of in vitro pre-vascularization strategies. In this study, we aimed at optimizing the pro-angiogenic effect of Cobalt (Co2+ ) to enhance vascular endothelial growth factor (VEGF) expression by human periosteum-derived mesenchymal stem cells (hPDCs). Simultaneously we set out to promote microvascular network formation by co-culturing with human umbilical vein endothelial cells (HUVECs)...
April 4, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29626693/antibiotic-resistance-in-wastewater-treatment-plants-tackling-the-black-box
#12
REVIEW
Célia M Manaia, Jaqueline Rocha, Nazareno Scaccia, Roberto Marano, Elena Radu, Francesco Biancullo, Francisco Cerqueira, Gianuário Fortunato, Iakovos C Iakovides, Ian Zammit, Ioannis Kampouris, Ivone Vaz-Moreira, Olga C Nunes
Wastewater is among the most important reservoirs of antibiotic resistance in urban environments. The abundance of carbon sources and other nutrients, a variety of possible electron acceptors such as oxygen or nitrate, the presence of particles onto which bacteria can adsorb, or a fairly stable pH and temperature are examples of conditions favouring the remarkable diversity of microorganisms in this peculiar habitat. The wastewater microbiome brings together bacteria of environmental, human and animal origins, many harbouring antibiotic resistance genes (ARGs)...
April 4, 2018: Environment International
https://www.readbyqxmd.com/read/29625748/engineering-approaches-to-understanding-mechanisms-of-spinal-column-injury-leading-to-spinal-cord-injury
#13
REVIEW
Claire F Jones, Elizabeth C Clarke
BACKGROUND: The mechanical interactions occurring between the spinal column and spinal cord during an injury event are complex and variable, and likely have implications for the clinical presentation and prognosis of the individual. METHODS: The engineering approaches that have been developed to better understand spinal column and cord interactions during an injury event are discussed. These include injury models utilising human and animal cadaveric specimens, in vivo anaesthetised animals, finite element models, inanimate physical systems and combinations thereof...
March 28, 2018: Clinical Biomechanics
https://www.readbyqxmd.com/read/29625575/targeted-genome-engineering-in-human-induced-pluripotent-stem-cells-from-patients-with-hemophilia-b-using-the-crispr-cas9-system
#14
Cuicui Lyu, Jun Shen, Rui Wang, Haihui Gu, Jianping Zhang, Feng Xue, Xiaofan Liu, Wei Liu, Rongfeng Fu, Liyan Zhang, Huiyuan Li, Xiaobing Zhang, Tao Cheng, Renchi Yang, Lei Zhang
BACKGROUND: Replacement therapy for hemophilia remains a lifelong treatment. Only gene therapy can cure hemophilia at a fundamental level. The clustered regularly interspaced short palindromic repeats-CRISPR associated nuclease 9 (CRISPR-Cas9) system is a versatile and convenient genome editing tool which can be applied to gene therapy for hemophilia. METHODS: A patient's induced pluripotent stem cells (iPSCs) were generated from their peripheral blood mononuclear cells (PBMNCs) using episomal vectors...
April 6, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29621998/a-factor-xia-activatable-hirudin-albumin-fusion-protein-reduces-thrombosis-in-mice-without-promoting-blood-loss
#15
William P Sheffield, Louise J Eltringham-Smith, Varsha Bhakta
BACKGROUND: Hirudin is a potent thrombin inhibitor but its antithrombotic properties are offset by bleeding side-effects. Because hirudin's N-terminus must engage thrombin's active site for effective inhibition, fusing a cleavable peptide at this site may improve hirudin's risk/benefit ratio as a therapeutic agent. Previously we engineered a plasmin cleavage site (C) between human serum albumin (HSA) and hirudin variant 3 (HV3) in fusion protein HSACHV3. Because coagulation factor XI (FXI) is more involved in thrombosis than hemostasis, we hypothesized that making HV3 activity FXIa-dependent would also improve HV3's potential therapeutic profile...
April 5, 2018: BMC Biotechnology
https://www.readbyqxmd.com/read/29619243/two-dimensional-material-based-bionano-platforms-to-control-mesenchymal-stem-cell-differentiation
#16
REVIEW
Ee-Seul Kang, Da-Seul Kim, Intan Rosalina Suhito, Wanhee Lee, Inbeom Song, Tae-Hyung Kim
Background: In the past decade, stem cells, with their ability to differentiate into various types of cells, have been proven to be resourceful in regenerative medicine and tissue engineering. Despite the ability to repair damaged parts of organs and tissues, the use of stem cells still entails several limitations, such as low differentiation efficiency and difficulties in guiding differentiation. To address these limitations, nanotechnology approaches have been recently implemented in stem cell research...
2018: Biomaterials Research
https://www.readbyqxmd.com/read/29615700/affinity-maturation-of-humanized-anti-epidermal-growth-factor-receptor-antibody-using-a-modified-phage-based-open-sandwich-selection-method
#17
Hideaki Sanada, Kazuki Kobayashi, Kenji Oyama, Takamitsu Maru, Takeshi Nakanishi, Mitsuo Umetsu, Ryutaro Asano, Izumi Kumagai
Affinity maturation is one of the cardinal strategies for improving antibody function using in vitro evolutionary methods; one such well-established method is phage display. To minimise gene deletion, we previously developed an open sandwich (OS) method wherein selection was performed using only phage-displaying VH fragments after mixing with soluble VL fragments. The decrease in anti-EGFR antibody 528 affinity through humanization was successfully recovered by selecting VH mutants using this OS method. However, the affinity was not similar to that of parental 528...
April 3, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29615469/thrombopoietin-protects-hematopoietic-stem-cells-from-retrotransposon-mediated-damage-by-promoting-an-antiviral-response
#18
Daniela Barbieri, Emilie Elvira-Matelot, Yanis Pelinski, Laetitia Genève, Bérengère de Laval, Gayathri Yogarajah, Christian Pecquet, Stefan N Constantinescu, Françoise Porteu
Maintenance of genomic integrity is crucial for the preservation of hematopoietic stem cell (HSC) potential. Retrotransposons, spreading in the genome through an RNA intermediate, have been associated with loss of self-renewal, aging, and DNA damage. However, their role in HSCs has not been addressed. Here, we show that mouse HSCs express various retroelements (REs), including long interspersed element-1 (L1) recent family members that further increase upon irradiation. Using mice expressing an engineered human L1 retrotransposition reporter cassette and reverse transcription inhibitors, we demonstrate that L1 retransposition occurs in vivo and is involved in irradiation-induced persistent γH2AX foci and HSC loss of function...
April 3, 2018: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/29615126/mesenchymal-stem-cells-and-extracellular-matrix-scaffold-promote-muscle-regeneration-by-synergistically-regulating-macrophage-polarization-toward-the-m2-phenotype
#19
Xinyu Qiu, Shiyu Liu, Hao Zhang, Bin Zhu, Yuting Su, Chenxi Zheng, Rong Tian, Miao Wang, Huijuan Kuang, Xinyi Zhao, Yan Jin
BACKGROUND: Skeletal muscle plays an important role in the body's physiology but there are still no effective treatments for volumetric muscle loss (VML) resulting from severe traumatic injury or tumor excision. Recent studies show that a tissue engineering strategy using a compound containing mesenchymal stem cells (MSCs) and decellularized extracellular matrix (ECM) scaffold generates significant regenerative effects on VML injury, but the underlying mechanisms are not fully understood...
April 3, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29614431/3d-cell-printing-of-in-vitro-stabilized-skin-model-and-in-vivo-pre-vascularized-skin-patch-using-tissue-specific-extracellular-matrix-bioink-a-step-towards-advanced-skin-tissue-engineering
#20
Byoung Soo Kim, Yang Woo Kwon, Jeong-Sik Kong, Gyu Tae Park, Ge Gao, Wonil Han, Moon-Bum Kim, Hyungseok Lee, Jae Ho Kim, Dong-Woo Cho
3D cell-printing technique has been under spotlight as an appealing biofabrication platform due to its ability to precisely pattern living cells in pre-defined spatial locations. In skin tissue engineering, a major remaining challenge is to seek for a suitable source of bioink capable of supporting and stimulating printed cells for tissue development. However, current bioinks for skin printing rely on homogeneous biomaterials, which has several shortcomings such as insufficient mechanical properties and recapitulation of microenvironment...
March 23, 2018: Biomaterials
keyword
keyword
70490
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"