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https://www.readbyqxmd.com/read/29242161/preparation-of-dexamethasone-loaded-biphasic-calcium-phosphate-nanoparticles-collagen-porous-composite-scaffolds-for-bone-tissue-engineering
#1
Ying Chen, Naoki Kawazoe, Guoping Chen
Although bone is regenerative, its regeneration capacity is limited. For bone defects beyond a critical size, further intervention is required. As an attractive strategy, bone tissue engineering (bone TE) has been widely investigated to repair bone defects. However, the rapid and effective bone regeneration of large non-healing defects is still a great challenge. Multifunctional scaffolds having osteoinductivity and osteoconductivity are desirable to fasten functional bone tissue regeneration. In the present study, biomimetic composite scaffolds of collagen and biphasic calcium phosphate nanoparticles (BCP NPs) with a controlled release of dexamethasone (DEX) and the controlled pore structures were prepared for bone TE...
December 11, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29240625/orbital-shear-stress-regulates-differentiation-and-barrier-function-of-primary-renal-tubular-epithelial-cells
#2
Nicholas Ferrell, Jin Cheng, Simeng Miao, Shuvo Roy, William H Fissell
Primary cells cultured in vitro gradually lose features characteristic of the in vivo phenotype. Culture techniques that help maintain cell-specific phenotype are advantageous for development of tissue engineered and bioartificial organs. Here we evaluated the phenotype of primary human renal tubule epithelial cells subjected to fluid shear stress by culturing the cells on an orbital shaker. Transepithelial electrical resistance (TEER), cell density, and gene and protein expression of proximal tubule-specific functional markers were measured in cells subjected to orbital shear stress...
December 11, 2017: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/29240313/deconvolution-of-images-from-3d-printed-cells-in-layers-on-a-chip
#3
Sean Yu, Pranav Joshi, Yi Ju Park, Kyeong-Nam Yu, Moo-Yeal Lee
Layer-by-layer cell printing is useful in mimicking layered tissue structures inside the human body and has great potential for being a promising tool in the field of tissue engineering, regenerative medicine, and drug discovery. However, imaging human cells cultured in multiple hydrogel layers in 3D-printed tissue constructs is challenging as the cells are not in a single focal plane. Although confocal microscopy could be a potential solution for this issue, it compromises the throughput which is a key factor in rapidly screening drug efficacy and toxicity in pharmaceutical industries...
December 14, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/29239153/time-to-change-the-classical-vision-of-coagulation-in-liver-disease-from-the-balance-disequilibrium-to-the-systems-biology-network-modelling
#4
Annabel Blasi, Joan C Reverter
The longstanding dogma that patients with liver disease have a hemostasis related bleeding tendency is neglected from the last 10 years however, despite this tremendous change of paradigm no longer progress had been made in this field. A mathematical model is a description of a system using mathematical concepts and language. Mathematical models are able to analyze large amount of data and they can make predictions about behavior. Mathematical models are used in the social sciences, engineering as well as in medicine...
December 13, 2017: Minerva Anestesiologica
https://www.readbyqxmd.com/read/29237829/unexpected-functional-divergence-of-bat-influenza-virus-ns1-proteins
#5
Hannah L Turkington, Mindaugas Juozapaitis, Nikos Tsolakos, Eugenia Corrales-Aguilar, Martin Schwemmle, Benjamin G Hale
Recently, two influenza A virus (FLUAV) genomes were identified in Central and South American bats. These sequences exhibit notable divergence from classical FLUAV counterparts, and functionally, bat FLUAV glycoproteins lack canonical receptor binding and destroying activity. Nevertheless, other features that distinguish these viruses from classical FLUAVs have yet to be explored. Here, we studied the viral non-structural protein, NS1, a virulence factor that modulates host signaling to promote efficient propagation...
December 13, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29235474/engineering-cell-signaling-using-tunable-crispr-cpf1-based-transcription-factors
#6
Yuchen Liu, Jinghong Han, Zhicong Chen, Hanwei Wu, Hongsong Dong, Guohui Nie
The catalytically dead Cpf1 endonuclease from Acidaminococcus sp. BV3L6 (dAsCpf1) has been used to construct effective transcriptional repressors in bacteria and plants. However, it is still unclear if dAsCpf1 can function in human cells as a transcriptional regulator or a signal conductor. Here, we repurpose the dAsCpf1 system in human cells for a variety of functions, including the activation or repression of gene transcription. Moreover, we construct programmable ligand-controlled dAsCpf1 systems either by coupling crRNAs with engineered riboswitches or by fusing dAsCpf1 proteins with G protein-coupled receptors...
December 13, 2017: Nature Communications
https://www.readbyqxmd.com/read/29234944/investigating-the-mincing-method-for-isolation-of-adipose-derived-stem-cells-from-pregnant-women-fat
#7
Yuan-Sheng Li, Pao-Jen Chen, Li-Wei Wu, Pei-Wen Chou, Li-Yi Sun, Tzyy-Wen Chiou
The success of stem cell application in regenerative medicine, usually require a stable source of stem or progenitor cells. Fat tissue represents a good source of stem cells because it is rich in stem cells and there are fewer ethical issues related to the use of such stem cells, unlike embryonic stem cells. Therefore, there has been increased interest in adipose-derived stem cells (ADSCs) for tissue engineering applications. Here, we aim to provide an easy processing method for isolating adult stem cells from human adipose tissue harvested from the subcutaneous fat of the abdominal wall during gynecologic surgery...
December 12, 2017: Cytotechnology
https://www.readbyqxmd.com/read/29234895/intestinal-stem-cells-to-advance-drug-development-precision-and-regenerative-medicine-a-paradigm-shift-in-translational-research
#8
Jonathan P Mochel, Albert E Jergens, Dawn Kingsbury, Hyun Jung Kim, Martín G Martín, Karin Allenspach
Recent advances in our understanding of the intestinal stem cell niche and the role of key signaling pathways on cell growth and maintenance have allowed the development of fully differentiated epithelial cells in 3D organoids. Stem cell-derived organoids carry significant levels of proteins that are natively expressed in the gut and have important roles in drug transport and metabolism. They are, therefore, particularly relevant to study the gastrointestinal (GI) absorption of oral medications. In addition, organoids have the potential to serve as a robust preclinical model for demonstrating the effectiveness of new drugs more rapidly, with more certainty, and at lower costs compared with live animal studies...
December 12, 2017: AAPS Journal
https://www.readbyqxmd.com/read/29234606/complete-genome-sequence-of-n2-fixing-model-strain-klebsiella-sp-nov-m5al-which-produces-plant-cell-wall-degrading-enzymes-and-siderophores
#9
Zhili Yu, Shuying Li, Yuanyuan Li, Zhuang Jiang, Jinru Zhou, Qianli An
The bacterial strain M5al is a model strain for studying the molecular genetics of N2-fixation and molecular engineering of microbial production of platform chemicals 1,3-propanediol and 2,3-butanediol. Here, we present the complete genome sequence of the strain M5al, which belongs to a novel species closely related to Klebsiella michiganensis. M5al secretes plant cell wall-degrading enzymes and colonizes rice roots but does not cause soft rot disease. M5al also produces siderophores and contains the gene clusters for synthesis and transport of yersiniabactin which is a critical virulence factor for Klebsiella pathogens in causing human disease...
March 2018: Biotechnology Reports
https://www.readbyqxmd.com/read/29234013/the-differentiation-stage-of-transplanted-stem-cells-modulates-nerve-regeneration
#10
Ching-Wen Huang, Wen-Chin Huang, Xuefeng Qiu, Flavia Fernandes Ferreira da Silva, Aijun Wang, Shyam Patel, Leon J Nesti, Mu-Ming Poo, Song Li
In regenerative medicine applications, the differentiation stage of implanted stem cells must be optimized to control cell fate and enhance therapeutic efficacy. We investigated the therapeutic potential of human induced pluripotent stem cell (iPSC)-derived cells at two differentiation stages on peripheral nerve regeneration. Neural crest stem cells (NCSCs) and Schwann cells (NCSC-SCs) derived from iPSCs were used to construct a tissue-engineered nerve conduit that was applied to bridge injured nerves in a rat sciatic nerve transection model...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29230672/the-role-of-b-cell-maturation-antigen-in-the-biology-and-management-of-and-as-a-potential-therapeutic-target-in-multiple-myeloma
#11
Eric Sanchez, Emily J Smith, Moryel A Yashar, Saurabh Patil, Mingjie Li, Autumn L Porter, Edward J Tanenbaum, Remy E Schlossberg, Camilia M Soof, Tara Hekmati, George Tang, Cathy S Wang, Haiming Chen, James R Berenson
B-cell maturation antigen (BCMA) was originally identified as a cell membrane receptor, expressed exclusively on late stage B-cells and plasma cells (PCs). Investigations of BCMA as a target for therapeutic intervention in multiple myeloma (MM) were initiated in 2007, using cSG1 as a naked antibody (Ab) as well as an Ab-drug conjugate (ADC) targeting BCMA, ultimately leading to ongoing clinical studies for previously treated MM patients. Since then, multiple companies have developed anti-BCMA-directed ADCs...
December 11, 2017: Targeted Oncology
https://www.readbyqxmd.com/read/29230433/proangiogenic-activity-of-endometrial-epithelial-and-stromal-cells-in-response-to-estradiol-in-gelatin-hydrogels
#12
Jacquelyn C Pence, Kathryn B H Clancy, Brendan A C Harley
Biomaterial vascularization remains a major focus in the field of tissue engineering. Biomaterial culture of endometrial cells is described as a platform to inform the design of proangiogenic biomaterials. The endometrium undergoes rapid growth and shedding of dense vascular networks during each menstrual cycle mediated via estradiol and progesterone in vivo. Cocultures of endometrial epithelial and stromal cells encapsulated within a methacrylamide-functionalized gelatin hydrogel are employed. It is reported that proangiogenic gene expression profiles and vascular endothelial growth factor production are hormone dependent in endometrial epithelial cells, but that hormone signals have no effect on human telomerase reverse transcriptase (hTERT)-immortalized endometrial stromal cells...
September 2017: Advanced Biosystems
https://www.readbyqxmd.com/read/29230380/the-axolotl-limb-regeneration-model-as-a-discovery-tool-for-engineering-the-stem-cell-niche
#13
Negar Seyedhassantehrani, Takayoshi Otsuka, Shambhavi Singh, David M Gardiner
Purpose of review: Recent advances in genomics and gene editing have expanded the range of model organisms to include those with interesting biological capabilities such as regeneration. Among these are the classic models of regeneration biology, the salamander. Although stimulating endogenous regeneration in humans likely is many years away, with advances in stem cell biology and biomedical engineering (e.g. bio-inspired materials), it is evident that there is great potential to enhance regenerative outcomes by approaching the problem from an engineering perspective...
September 2017: Current Stem Cell Reports
https://www.readbyqxmd.com/read/29229979/near-infrared-plasmonic-assemblies-of-gold-nanoparticles-with-multimodal-function-for-targeted-cancer-theragnosis
#14
Seong-Eun Kim, Bo-Ram Lee, Hohyeon Lee, Sung Duk Jo, Hyuncheol Kim, You-Yeon Won, Jeewon Lee
Here we report a novel assembly structure of near-infrared plasmonic gold nanoparticles (AuNPs), possessing both photoacoustic (PA) and photothermal (PT) properties. The template for the plasmonic AuNP assembly is a bioconjugate between short double-strand DNA (sh-dsDNA) and human methyl binding domain protein 1 (MBD1). MBD1 binds to methylated cytosine-guanine dinucleotides (mCGs) within the sequence of sh-dsDNA. Hexahistidine peptides on the engineered MBD1 function as a nucleation site for AuNP synthesis, allowing the construction of hybrid conjugates, sh-dsDNA-MBD1-AuNPs (named DMAs)...
December 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29227558/rna-sequencing-identifies-gene-regulatory-networks-controlling-extracellular-matrix-synthesis-in-intervertebral-disk-tissues
#15
Scott M Riester, Yang Lin, Wei Wang, Lin Cong, Abdel-Moneim Mohamed Ali, Sun H Peck, Lachlan J Smith, Bradford L Currier, Michelle Clark, Paul Huddleston, William Krauss, Michael J Yaszemski, Mark E Morrey, Matthew P Abdel, Mohamad Bydon, Wenchun Qu, A Noelle Larson, Andre J van Wijnen, Ahmad Nassr
Degenerative disk disease of the spine is a major cause of back pain and disability. Optimization of regenerative medical therapies for degenerative disk disease requires a deep mechanistic understanding of the factors controlling the structural integrity of spinal tissues. In this investigation, we sought to identify candidate regulatory genes controlling extracellular matrix synthesis in spinal tissues. To achieve this goal we performed high throughput next generation RNA sequencing on 39 annulus fibrosus and 21 nucleus pulposus human tissue samples...
December 11, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/29227181/effects-of-fuel-components-and-combustion-particle-physicochemical-properties-on-toxicological-responses-of-lung-cells
#16
Isabel C Jaramillo, Anne Sturrock, Hossein Ghiassi, Diana J Woller, Cassandra E Deering-Rice, JoAnn S Lighty, Robert Paine, Christopher Reilly, Kerry E Kelly
The physicochemical properties of combustion particles that promote lung toxicity are not fully understood, hindered by the fact that combustion particles vary based on the fuel and combustion conditions. Real-world combustion-particle properties also continually change as new fuels are implemented, engines age, and engine technologies evolve. This work used laboratory-generated particles produced under controlled combustion conditions in an effort to understand the relationship between different particle properties and the activation of established toxicological outcomes in human lung cells (H441 and THP-1)...
December 11, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/29225642/comparison-of-apical-transportation-with-the-use-of-rotary-system-and-reciprocating-handpiece-with-precurved-hand-files-an-in-vitro-study
#17
Fatemeh Mohammadian, Atefeh Sadeghi, Fatemeh Dibaji, Mona Sadegh, Zahra Ghoncheh, Mohammad Javad Kharrazifard
Introduction: Success of root canal treatment depends on several factors; among which, maintaining the original canal path during mechanical preparation is extremely important. This in vitro study aimed to compare apical transportation using RaCe NiTi rotary system and precurved stainless steel (SS) hand files in a reciprocating handpiece. Methods and Materials: Mesiobuccal canals of 40 extracted human mandibular first and second molars with 20 to 45° curvatures and 3 to 7 mm curve radius were chosen for this study...
2017: Iranian Endodontic Journal
https://www.readbyqxmd.com/read/29225457/corneal-regeneration-by-induced-human-buccal-mucosa-cultivated-on-an-amniotic-membrane-following-alkaline-injury
#18
Rohaina Che Man, Then Kong Yong, Ng Min Hwei, Wan Haslina Wan Abdul Halim, Aida Zairani Mohd Zahidin, Roszalina Ramli, Aminuddin Bin Saim, Ruszymah Binti Hj Idrus
Various clinical disorders and injuries, such as chemical, thermal, or mechanical injuries, may lead to corneal loss that results in blindness. PURPOSE: The aims of this study were to differentiate human buccal mucosa (BMuc) into corneal epithelial-like cells, to fabricate engineered corneal tissue using buccal mucosal epithelial cells, and to reconstruct a damaged corneal epithelium in a nude rat model. Methods: BMuc were subjected to 10 d of induction factors to investigate the potential of cells to differentiate into corneal lineages...
2017: Molecular Vision
https://www.readbyqxmd.com/read/29222839/decellularized-human-liver-extracellular-matrix-hdlm-mediated-hepatic-differentiation-of-human-induced-pluripotent-stem-cells-hipsc
#19
Maria Jaramillo, Heidi Yeh, Martin L Yarmush, Basak E Uygun
Liver tissue engineering has emerged as a promising approach in organ transplantation, but has been hampered by the lack of a reliable and readily available cell source. Induced pluripotent cells (hiPSC) have been highlighted a desirable source, due to their differentiation potential, ability to self-renew and the possibility of making patient specific cells. We developed a decellularization protocol that efficiently removes cellular material, while retaining extracellular matrix components. Subsequently, hiPSC were differentiated on the decellularized human liver matrix (hDLM) scaffolds using an established hepatic differentiation protocol...
December 8, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29222786/nerve-growth-factor-induced-angiogenesis-1-endothelial-cell-tube-formation-assay
#20
Philip Lazarovici, Adi Lahiani, Galit Gincberg, Dikla Haham, Arnon Fluksman, Ofra Benny, Cezary Marcinkiewicz, Peter I Lelkes
Nerve growth factor (NGF) is a neurotrophin promoting survival, proliferation, differentiation, and neuroprotection in the embryonal and adult nervous system. NGF also induces angiogenic effects in the cardiovascular system, which may be beneficial in engineering new blood vessels and for developing novel anti-angiogenesis therapies for cancer. Angiogenesis is a cellular process characterized by a number of events, including endothelial cell migration, invasion, and assembly into capillaries. In vitro endothelial tube formation assays are performed using primary human umbilical vein endothelial cells, human aortic endothelial cells, and other human or rodent primary endothelial cells isolated from the vasculature of both tumors and normal tissues...
2018: Methods in Molecular Biology
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