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https://www.readbyqxmd.com/read/28766300/a-cell-based-high-throughput-method-for-identifying-modulators-of-alternative-splicing
#1
Sika Zheng
Alternative splicing, a key regulatory process of gene expression, is controlled by trans-acting factors that recognize cis-elements in premature RNA transcripts to affect spliceosome assembly and splice site choices. Extracellular stimuli and signaling cascades can converge on RNA binding splicing regulators to affect alternative splicing. Defects in splicing regulation have been associated with various human diseases, and modification of disease-causing splicing events presents great therapeutic promise. Determining splicing regulators and/or upstream modulators has been difficult with low throughput, low sensitivity, and low specificity...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28765719/time-series-data-of-a-broadleaved-secondary-forest-in-japan-as-affected-by-deer-and-mass-mortality-of-oak-trees
#2
Hiroki Itô
BACKGROUND: Abandonment of broadleaved secondary forests that have been used for various purposes may cause the loss of biodiversity. Some of these forests suffer from diseases such as Japanese oak wilt. An increasing number of deer also impact some of them. Monitoring and recording the status of such forests is important for their proper management. NEW INFORMATION: This data set provides a concrete example of temporal changes in a temperate broadleaved secondary forest...
2017: Biodiversity Data Journal
https://www.readbyqxmd.com/read/28758428/the-proteins-from-sika-deer-antler-as-potential-modulators-on-cisplatin-induced-cytotoxicity-in-human-embryonic-kidney-293-cells
#3
Huihai Yang, Wei Li, Lulu Wang, Xiaofeng He, Hang Sun, Jing Zhang
Our study aimed to investigate the protective role of SDAPR on cisplatin-induced cytotoxicity and its' possible mechanism in HEK293 cells. Cell viability was measured by MTT assay. Oxidative stress (SOD, GSH, LDH and MDA), inflammatory factors (TNF-α and IL-6) and apoptosis-related proteins (caspase-3, Bax, Bcl-2) expression were measured. The apoptotic cells were observed by TUNEL staining. Our study results indicated that non-cytotoxic levels of SDAPR significantly increased viability rate (LD50 value of cisplatin is 20 μM), which improved antioxidant defence, attenuated apoptosis by decreasing expression levels of cleaved-caspase-3 and Bax, increasing Bcl-2 expression and inhibiting apoptotic positive cells in HEK 293 cells...
July 31, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28751500/genome-wide-snp-discovery-and-analysis-of-genetic-diversity-in-farmed-sika-deer-cervus-nippon-in-northeast-china-using-double-digest-restriction-site-associated-dna-sequencing
#4
Hengxing Ba, Boyin Jia, Guiwu Wang, Yifeng Yang, Gilead Kedem, Chunyi Li
Sika deer are an economically valuable species for their use in traditional Chinese medicine, particularly velvet antlers. Sika deer in northeast China are mostly farmed in enclosure. Therefore, genetic management of farmed sika deer would benefit from detailed knowledge of their genetic diversity. In this study, we generated over 1.45 billion high-quality paired-end reads (288 Gbp) across 42 unrelated individuals using double-digest restriction-site associated DNA sequencing (ddRAD-seq). A total of 96,188 (29...
July 27, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28749086/1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol-plag-reduces-hepatic-injury-in-concanavalin-a-treated-mice
#5
Young-Eun Ko, Sun Young Yoon, Sun-Yung Ly, Joo-Heon Kim, Ki-Young Sohn, Jae Wha Kim
1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (PLAG), a chemically synthesized monoacetyldiaglyceride, is one of the constituents in Sika deer antlers and has been known traditionally as having immunomodulatory effects. However, the mechanism by which PLAG controls neutrophil migration, which evokes liver injury in the hepatitis animal model, remains largely unknown. This study was designed to evaluate the immunomodulatory effects of PLAG on cytokine secretion and neutrophil migration in vivo and in vitro. The injection of mice with Concanavalin A (Con A) induced leukocyte infiltration in the liver and increased plasma cytokine levels...
July 27, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28744165/asymptomatic-multinodular-splenoma-splenic-hamartoma-in-a-child-with-sickle-cell-anemia
#6
Narcisse Elenga, Sylvain Labbé, Nicolas Leduc, Anicet Sika, Emma Cuadro, Laurence Long, Falucar Njuieyon, Rémi Kom-Tchameni, Thierry Basset
Splenoma is a rare and benign malformation usually fortuitously diagnosed during imaging, surgery or, unfortunately, at autopsy. Although splenoma was first described in 1861, its association with hematological pathology is a very unusual condition in children. We report the case of an asymptomatic splenoma in an 8-year-old boy with sickle cell anemia, whose diagnosis was confirmed after conventional splenectomy.
2017: International Medical Case Reports Journal
https://www.readbyqxmd.com/read/28735469/phylogenetic-relationships-between-dicrocoelium-chinensis-populations-in-japan-and-china-based-on-mitochondrial-nad1-gene-sequences
#7
Kei Hayashi, WenQiang Tang, Yuma Ohari, Maiko Ohtori, Uday Kumar Mohanta, Kayoko Matsuo, Hiroshi Sato, Tadashi Itagaki
We carried out phylogenetic analyses of the relationships between Dicrocoelium chinensis populations in Japan and China using molecular markers. One hundred nine lancet flukes collected from Japan and China were identified as D. chinensis based on their testis orientation and the nucleotide sequences of their ribosomal ITS2. These flukes were analyzed phylogenetically using mitochondrial nad1 gene sequences. An analysis of molecular variance found that the percentage of variation between the countries was extremely high, indicating that the D...
July 22, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28701625/first-report-of-fasciola-larva-infection-in-galbatruncatula-m%C3%A3-ller-1774-gastropoda-lymnaeidae-occurring-in-the-natural-environment-in-hokkaido-japan
#8
Yuma Ohari, Kei Hayashi, Uday Kumar Mohanta, Yasuhiro Kuwahara, Tadashi Itagaki
In Hokkaido, Japan, wild sika deer are highly infected with Fasciola flukes, suggesting that the flukes complete their life cycle via intermediate host snails and definitive host animals occurring in the natural environment. However, infected snails have been found only in cattle farms contaminated with fasciolosis. This study reports the first Fasciola larva infection in Galbatruncatula snails occurring in the Shoro and Atsuma rivers in the natural environment. Molecular analysis revealed that the nad1 haplotype of the larvae was consistent with that of Fasciola adults obtained from sika deer in Hokkaido...
July 13, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28669001/long-term-deer-exclosure-alters-soil-properties-plant-traits-understory-plant-community-and-insect-herbivory-but-not-the-functional-relationships-among-them
#9
Jörg G Stephan, Fereshteh Pourazari, Kristina Tattersdill, Takuya Kobayashi, Keita Nishizawa, Jonathan R De Long
Evidence of the indirect effects of increasing global deer populations on other trophic levels is increasing. However, it remains unknown if excluding deer alters ecosystem functional relationships. We investigated how sika deer exclosure after 18 years changed soil conditions, the understory plant community, the traits of a dominant understory plant (Sasa palmata), herbivory by three insect-feeding guilds, and the functional relationships between these properties. Deer absence decreased understory plant diversity, but increased soil organic matter and ammonium concentrations...
July 2017: Oecologia
https://www.readbyqxmd.com/read/28651947/molecular-identification-and-characterization-of-piroplasm-species-in-hokkaido-sika-deer-cervus-nippon-yesoensis-japan
#10
Elzahara Elbaz, Mohamed Abdallah Mohamed Moustafa, Kyunglee Lee, Wessam Mohamed Ahmed Mohamed, Ryo Nakao, Michito Shimozuru, Mariko Sashika, Emad Elsayed Ahmed Younis, Sabry Ahmed El-Khodery, Toshio Tsubota
Babesia and Theileria species are tick-borne protozoan parasites that have a veterinary and zoonotic importance. In order to investigate the prevalence and genetic diversity of these parasites, a total of 269 sika deer blood DNA samples collected from Hokkaido, Japan, were examined for Babesia and Theileria species by touch-down PCR targeting the 18S rRNA gene. Reverse line blot (RLB) hybridization was then used to detect 12 piroplasm species. The results revealed that 95.5% (257/269), 94.1% (253/269), 14.1% (38/269), 87...
August 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28631344/evaluation-of-the-genetic-structure-of-sika-deer-cervus-nippon-in-japan-s-kanto-and-tanzawa-mountain-areas-based-on-microsatellite-markers
#11
Sayaka Konishi, Shoko Hata, Sayumi Matsuda, Kazushi Arai, Yasushi Mizoguchi
The browsing habits of sika deer (Cervus nippon) in Japan have caused serious ecological problems. Appropriate management of sika deer populations requires understanding the different genetic structures of local populations. In the present study, we used 10 microsatellite polymorphisms to explore the genetic structures of sika deer populations (162 individuals) living in the Kanto region. The expected heterozygosity of the Tanzawa mountain range population (Group I) was lower than that of the populations in the Kanto mountain areas (Group II)...
June 19, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28627038/estimating-the-number-of-female-sex-workers-in-c%C3%A3-te-d-ivoire-results-and-lessons-learned
#12
Bea Vuylsteke, Lazare Sika, Gisèle Semdé, Camille Anoma, Elise Kacou, Marie Laga
OBJECTIVE: To report on the results of three size estimations of the populations of female sex workers (FSW) in five cities in Côte d'Ivoire and on operational lessons learned, which may be relevant for key populations programs in other parts of the world. METHODS: We applied three methods: mapping and census, capture-recapture and service multiplier. All were applied between 2008 and 2009 in Abidjan, San Pedro, Bouaké, Yamoussoukro and Abengourou. RESULTS: Abidjan was the city with the highest number of FSW by far, with estimations between 7880 (census) and 13714 (service multiplier)...
June 19, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28572794/new-genotypes-of-enterocytozoon-bieneusi-isolated-from-sika-deer-and-red-deer-in-china
#13
Jianying Huang, Zhenjie Zhang, Yong Yang, Rongjun Wang, Jinfeng Zhao, Fuchun Jian, Changshen Ning, Longxian Zhang
To examine the occurrence and genotype distribution of Enterocytozoon bieneusi in cervids, 615 fecal samples were collected from red deer (Cervus elaphus) and sika deer (Cervus nippon) on 10 different farms in Henan and Jilin Province. Enterocytozoon bieneusi was identified and genotyped with a nested PCR analysis of the internal transcribed spacer (ITS) region of the rRNA genes, showing an average infection rate of 35.9% (221/615). In this study, 25 ITS genotypes were identified including seven known genotypes (BEB6, EbpC, EbpA, D, HLJDI, HLJD-IV, and COS-I) and 18 novel genotypes (designated JLD-I to JLD-XIV, HND-I to HND-IV)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28503294/a-survey-of-free-ranging-deer-in-ireland-for-serological-evidence-of-exposure-to-bovine-viral-diarrhoea-virus-bovine-herpes-virus-1-bluetongue-virus-and-schmallenberg-virus
#14
David A Graham, Clare Gallagher, Ruth F Carden, Jose-Maria Lozano, John Moriarty, Ronan O'Neill
BACKGROUND: Deer are an important wildlife species in both the Republic of Ireland and Northern Ireland having colonised most regions across the island of Ireland. In comparison to cattle and sheep which represent the main farmed ruminant species on the island, there is a lack of data concerning their exposure, as measured by the presence of antibodies, to important viral pathogens of ruminants. A study was therefore undertaken to investigate the seroprevalence of wild deer to four viruses, namely bovine viral diarrhoea virus (BVDV), bovine herpesvirus-1 (BoHV-1), Schmallenberg virus (SBV) and bluetongue virus (BTV)...
2017: Irish Veterinary Journal
https://www.readbyqxmd.com/read/28501503/detection-and-molecular-characterization-of-babesia-theileria-and-hepatozoon-species-in-hard-ticks-collected-from-kagoshima-the-southern-region-in-japan
#15
Tatsunori Masatani, Kei Hayashi, Masako Andoh, Morihiro Tateno, Yasuyuki Endo, Masahito Asada, Kodai Kusakisako, Tetsuya Tanaka, Mutsuyo Gokuden, Nodoka Hozumi, Fumiko Nakadohzono, Tomohide Matsuo
To reveal the distribution of tick-borne parasites, we established a novel nested polymerase chain reaction (PCR) system to detect the most common agents of tick-borne parasitic diseases, namely Babesia, Theileria, and Hepatozoon parasites. We collected host-seeking or animal-feeding ticks in Kagoshima Prefecture, the southernmost region of Kyusyu Island in southwestern Japan. Twenty of the total of 776 tick samples displayed a specific band of the appropriate size (approximately 1.4-1.6kbp) for the 18S rRNA genes in the novel nested PCR (20/776: 2...
March 27, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28396295/molecular-characterization-of-fasciola-flukes-obtained-from-wild-sika-deer-and-domestic-cattle-in-hokkaido-japan
#16
Madoka Ichikawa-Seki, Tomoko Shiroma, Tatsuya Kariya, Ryo Nakao, Yuma Ohari, Kei Hayashi, Shinya Fukumoto
The number of wild sika deer (Cervus nippon yesoensis) continues to increase in Hokkaido Prefecture, Japan. The major concern for the livestock industry is the transmission of pathogens between sika deer and cattle. Fasciolosis is an important disease that can occur in both animals. The aim of this study was to examine the possible mutual transmission of this disease in Hokkaido Prefecture. A total of 105 Fasciola flukes were obtained from sika deer and 96 from domestic cattle. The Fasciola flukes in Japan are reported to possess no mature sperm...
April 7, 2017: Parasitology International
https://www.readbyqxmd.com/read/28362864/epidemiological-study-of-relapsing-fever-borreliae-detected-in-haemaphysalis-ticks-and-wild-animals-in-the-western-part-of-japan
#17
Kiwa Furuno, Kyunglee Lee, Yukie Itoh, Kazuo Suzuki, Kenzo Yonemitsu, Ryusei Kuwata, Hiroshi Shimoda, Masahisa Watarai, Ken Maeda, Ai Takano
The genus Borrelia comprises arthropod-borne bacteria, which are infectious agents in vertebrates. They are mainly transmitted by ixodid or argasid ticks. In Hokkaido, Japan, Borrelia spp. were found in deer and Haemaphysalis ticks between 2011 and 2013; however, the study was limited to a particular area. Therefore, in the present study, we conducted large-scale surveillance of ticks and wild animals in the western part of the main island of Japan. We collected 6,407 host-seeking ticks from two regions and 1,598 larvae obtained from 32 engorged female ticks and examined them to elucidate transovarial transmission...
2017: PloS One
https://www.readbyqxmd.com/read/28335097/effects-of-dietary-forage-to-concentrate-ratio-on-nutrient-digestibility-and-enteric-methane-production-in-growing-goats-capra-hircus-hircus-and-sika-deer-cervus-nippon-hortulorum
#18
Youngjun Na, Dong Hua Li, Sang Rak Lee
OBJECTIVE: Two experiments were conducted to determine the effects of forage-to-concentrate (F:C) ratio on the nutrient digestibility and enteric methane (CH4) emission in growing goats and Sika deer. METHODS: Three male growing goats (body weight [BW] = 19.0±0.7 kg) and three male growing deer (BW = 19.3±1.2 kg) were respectively allotted to a 3×3 Latin square design with an adaptation period of 7 d and a data collection period of 3 d. Respiration-metabolism chambers were used for measuring the enteric CH4 emission...
July 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28320988/characterization-of-shiga-toxin-producing-escherichia-coli-from-feces-of-sika-deer-cervus-nippon-in-japan-using-pcr-binary-typing-analysis-to-evaluate-their-potential-human-pathogenicity
#19
Hidenori Kabeya, Shingo Sato, Shinya Oda, Megumi Kawamura, Mariko Nagasaka, Masanari Kuranaga, Eiji Yokoyama, Shinichiro Hirai, Atsushi Iguchi, Tomoe Ishihara, Toshiro Kuroki, Tomoko Morita-Ishihara, Sunao Iyoda, Jun Terajima, Makoto Ohnishi, Soichi Maruyama
This study examined the potential pathogenicity of Shiga toxin-producing Escherichia coli (STEC) in feces of sika deer by PCR binary typing (P-BIT), using 24 selected STEC genes. A total of 31 STEC strains derived from sika deer in 6 prefectures of Japan were O-serotyped and found to be O93 (n=12), O146 (n=5), O176 (n=3), O130 (n=3), O5 (n=2), O7 (n=1), O96 (n=1), O116 (n=1), O141 (n=1), O157 (n=1) and O-untypable (n=1). Of the 31 STEC strains, 13 carried both stx1 and stx2, 5 carried only stx1, and 13 carried one or two variants of stx2...
May 3, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28318383/detection-of-brucellosis-in-sika-deer-cervus-nippon-through-loop-mediated-isothermal-amplification-lamp
#20
Qianhong Liu, Jie Wei, Qingsong Sun, Ben Wang, Yuting Wang, Ying Hu, Wenrong Wu
Brucellosis (Brucella bovis) in sika deer ( Cervus nippon ) can cause enormous losses to stag breeding, especially in areas in which stag breeding has become an important industry. It also poses a threat to humans because it is a zoonotic disease. Use of the loop-mediated isothermal amplification (LAMP) assay has been poorly described in the diagnosis of brucellosis in deer. We developed a LAMP assay targeting the omp25 gene sequence to detect brucellosis in sika deer. The reaction can be completed in 60 min at 63 C and, with a detection limit of 17 pg, it was more sensitive than conventional PCR, with its detection limit of 1...
July 2017: Journal of Wildlife Diseases
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