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https://www.readbyqxmd.com/read/28631344/evaluation-of-the-genetic-structure-of-sika-deer-cervus-nippon-in-japan-s-kanto-and-tanzawa-mountain-areas-based-on-microsatellite-markers
#1
Sayaka Konishi, Shoko Hata, Sayumi Matsuda, Kazushi Arai, Yasushi Mizoguchi
The browsing habits of sika deer (Cervus nippon) in Japan have caused serious ecological problems. Appropriate management of sika deer populations requires understanding the different genetic structures of local populations. In the present study, we used 10 microsatellite polymorphisms to explore the genetic structures of sika deer populations (162 individuals) living in the Kanto region. The expected heterozygosity of the Tanzawa mountain range population (Group I) was lower than that of the populations in the Kanto mountain areas (Group II)...
June 19, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28627038/estimating-the-number-of-female-sex-workers-in-c%C3%A3-te-d-ivoire-results-and-lessons-learned
#2
Bea Vuylsteke, Lazare Sika, Gisèle Semdé, Camille Anoma, Elise Kacou, Marie Laga
OBJECTIVE: To report on the results of three size estimations of the populations of female sex workers (FSW) in five cities in Côte d'Ivoire and on operational lessons learned, which may be relevant for key populations programs in other parts of the world. METHODS: We applied three methods: mapping and census, capture-recapture and service multiplier. All were applied between 2008 and 2009 in Abidjan, San Pedro, Bouaké, Yamoussoukro and Abengourou. RESULTS: Abidjan was the city with the highest number of FSW by far, with estimations between 7880 (census) and 13714 (service multiplier)...
June 19, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28572794/new-genotypes-of-enterocytozoon-bieneusi-isolated-from-sika-deer-and-red-deer-in-china
#3
Jianying Huang, Zhenjie Zhang, Yong Yang, Rongjun Wang, Jinfeng Zhao, Fuchun Jian, Changshen Ning, Longxian Zhang
To examine the occurrence and genotype distribution of Enterocytozoon bieneusi in cervids, 615 fecal samples were collected from red deer (Cervus elaphus) and sika deer (Cervus nippon) on 10 different farms in Henan and Jilin Province. Enterocytozoon bieneusi was identified and genotyped with a nested PCR analysis of the internal transcribed spacer (ITS) region of the rRNA genes, showing an average infection rate of 35.9% (221/615). In this study, 25 ITS genotypes were identified including seven known genotypes (BEB6, EbpC, EbpA, D, HLJDI, HLJD-IV, and COS-I) and 18 novel genotypes (designated JLD-I to JLD-XIV, HND-I to HND-IV)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28503294/a-survey-of-free-ranging-deer-in-ireland-for-serological-evidence-of-exposure-to-bovine-viral-diarrhoea-virus-bovine-herpes-virus-1-bluetongue-virus-and-schmallenberg-virus
#4
David A Graham, Clare Gallagher, Ruth F Carden, Jose-Maria Lozano, John Moriarty, Ronan O'Neill
BACKGROUND: Deer are an important wildlife species in both the Republic of Ireland and Northern Ireland having colonised most regions across the island of Ireland. In comparison to cattle and sheep which represent the main farmed ruminant species on the island, there is a lack of data concerning their exposure, as measured by the presence of antibodies, to important viral pathogens of ruminants. A study was therefore undertaken to investigate the seroprevalence of wild deer to four viruses, namely bovine viral diarrhoea virus (BVDV), bovine herpesvirus-1 (BoHV-1), Schmallenberg virus (SBV) and bluetongue virus (BTV)...
2017: Irish Veterinary Journal
https://www.readbyqxmd.com/read/28501503/detection-and-molecular-characterization-of-babesia-theileria-and-hepatozoon-species-in-hard-ticks-collected-from-kagoshima-the-southern-region-in-japan
#5
Tatsunori Masatani, Kei Hayashi, Masako Andoh, Morihiro Tateno, Yasuyuki Endo, Masahito Asada, Kodai Kusakisako, Tetsuya Tanaka, Mutsuyo Gokuden, Nodoka Hozumi, Fumiko Nakadohzono, Tomohide Matsuo
To reveal the distribution of tick-borne parasites, we established a novel nested polymerase chain reaction (PCR) system to detect the most common agents of tick-borne parasitic diseases, namely Babesia, Theileria, and Hepatozoon parasites. We collected host-seeking or animal-feeding ticks in Kagoshima Prefecture, the southernmost region of Kyusyu Island in southwestern Japan. Twenty of the total of 776 tick samples displayed a specific band of the appropriate size (approximately 1.4-1.6kbp) for the 18S rRNA genes in the novel nested PCR (20/776: 2...
March 27, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28396295/molecular-characterization-of-fasciola-flukes-obtained-from-wild-sika-deer-and-domestic-cattle-in-hokkaido-japan
#6
Madoka Ichikawa-Seki, Tomoko Shiroma, Tatsuya Kariya, Ryo Nakao, Yuma Ohari, Kei Hayashi, Shinya Fukumoto
The number of wild sika deer (Cervus nippon yesoensis) continues to increase in Hokkaido Prefecture, Japan. The major concern for the livestock industry is the transmission of pathogens between sika deer and cattle. Fasciolosis is an important disease that can occur in both animals. The aim of this study was to examine the possible mutual transmission of this disease in Hokkaido Prefecture. A total of 105 Fasciola flukes were obtained from sika deer and 96 from domestic cattle. The Fasciola flukes in Japan are reported to possess no mature sperm...
April 7, 2017: Parasitology International
https://www.readbyqxmd.com/read/28362864/epidemiological-study-of-relapsing-fever-borreliae-detected-in-haemaphysalis-ticks-and-wild-animals-in-the-western-part-of-japan
#7
Kiwa Furuno, Kyunglee Lee, Yukie Itoh, Kazuo Suzuki, Kenzo Yonemitsu, Ryusei Kuwata, Hiroshi Shimoda, Masahisa Watarai, Ken Maeda, Ai Takano
The genus Borrelia comprises arthropod-borne bacteria, which are infectious agents in vertebrates. They are mainly transmitted by ixodid or argasid ticks. In Hokkaido, Japan, Borrelia spp. were found in deer and Haemaphysalis ticks between 2011 and 2013; however, the study was limited to a particular area. Therefore, in the present study, we conducted large-scale surveillance of ticks and wild animals in the western part of the main island of Japan. We collected 6,407 host-seeking ticks from two regions and 1,598 larvae obtained from 32 engorged female ticks and examined them to elucidate transovarial transmission...
2017: PloS One
https://www.readbyqxmd.com/read/28335097/effects-of-dietary-forage-to-concentrate-ratio-on-nutrient-digestibility-and-enteric-methane-production-in-growing-goats-capra-hircus-hircus-and-sika-deer-cervus-nippon-hortulorum
#8
Youngjun Na, Dong Hua Li, Sang Rak Lee
Two experiments were conducted to determine the effects of forage-to-concentrate (F:C) ratio on the nutrient digestibility and enteric methane (CH4) emission in growing goats and Sika deer. Three male growing goats (BW = 19.0 ± 0.7 kg) and three male growing deer (BW = 19.3 ± 1.2 kg) were respectively allotted to a 3 × 3 Latin square design with an adaptation period of 7 d and a data collection period of 3 d. Respiration-metabolism chambers were used for measuring the enteric CH4 emission. Treatments of low (25:75), moderate (50:50), and high (73:27) F:C ratios were given to both goats and Sika deer...
March 21, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28320988/characterization-of-shiga-toxin-producing-escherichia-coli-from-feces-of-sika-deer-cervus-nippon-in-japan-using-pcr-binary-typing-analysis-to-evaluate-their-potential-human-pathogenicity
#9
Hidenori Kabeya, Shingo Sato, Shinya Oda, Megumi Kawamura, Mariko Nagasaka, Masanari Kuranaga, Eiji Yokoyama, Shinichiro Hirai, Atsushi Iguchi, Tomoe Ishihara, Toshiro Kuroki, Tomoko Morita-Ishihara, Sunao Iyoda, Jun Terajima, Makoto Ohnishi, Soichi Maruyama
This study examined the potential pathogenicity of Shiga toxin-producing Escherichia coli (STEC) in feces of sika deer by PCR binary typing (P-BIT), using 24 selected STEC genes. A total of 31 STEC strains derived from sika deer in 6 prefectures of Japan were O-serotyped and found to be O93 (n=12), O146 (n=5), O176 (n=3), O130 (n=3), O5 (n=2), O7 (n=1), O96 (n=1), O116 (n=1), O141 (n=1), O157 (n=1) and O-untypable (n=1). Of the 31 STEC strains, 13 carried both stx1 and stx2, 5 carried only stx1, and 13 carried one or two variants of stx2...
May 3, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28318383/detection-of-brucellosis-in-sika-deer-cervus-nippon-through-loop-mediated-isothermal-amplification-lamp
#10
Qianhong Liu, Jie Wei, Qingsong Sun, Ben Wang, Yuting Wang, Ying Hu, Wenrong Wu
Brucellosis (Brucella bovis) in sika deer ( Cervus nippon ) can cause enormous losses to stag breeding, especially in areas in which stag breeding has become an important industry. It also poses a threat to humans because it is a zoonotic disease. Use of the loop-mediated isothermal amplification (LAMP) assay has been poorly described in the diagnosis of brucellosis in deer. We developed a LAMP assay targeting the omp25 gene sequence to detect brucellosis (Brucella bovis) in sika deer. The reaction can be completed in 60 min at 63 C and, with a detection limit of 17 pg, it was more sensitive than conventional PCR, with its detection limit of 1...
March 20, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28255918/morphological-and-molecular-characterization-of-four-sarcocystis-spp-including-sarcocystis-linearis-n-sp-from-roe-deer-capreolus-capreolus-in-italy
#11
Bjørn Gjerde, Stefano Giacomelli, Alessandro Bianchi, Irene Bertoletti, Hajime Mondani, Lucia Rita Gibelli
Fresh (frozen/thawed) muscle samples from four 2-12-year-old roe deer (Capreolus capreolus) from the Sondrio province in north-eastern Italy were examined under a dissecting microscope, and about 180 sarcocysts were isolated and identified to morphological type in wet mounts by light microscopy (LM). Seventy-seven of these sarcocysts were subsequently examined by molecular methods, comprising polymerase chain reaction (PCR) amplification and sequencing of the partial cytochrome c oxidase subunit I gene (cox1) of all isolates, as well as PCR amplification, cloning and sequencing of the complete18S ribosomal RNA (rRNA) gene of two isolates of each species found...
March 2, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28217218/high-prevalence-of-hepatitis-b-virus-infection-in-the-age-range-of-20-39-years-old-individuals-in-lome
#12
Malewe Kolou, Gnatoulma Katawa, Mounerou Salou, Komlan Selom Gozo-Akakpo, Sika Dossim, Alexander Kwarteng, Mireille Prince-David
BACKGROUND: Hepatitis B is a liver infection caused by the hepatitis B virus (HBV). It affects all women and men irrespective of age. Although sub-Saharan Africa is an area of high prevalence of this disease, data on the prevalence of acute and chronic HBV infections in this region remain to be widely documented. OBJECTIVE: This study aimed to investigate the prevalence of HBV in relation to age in Centre Hospitalier Universitaire Campus (CHU-C), one of the two teaching hospitals of Lome, Togo...
2017: Open Virology Journal
https://www.readbyqxmd.com/read/28204806/paediatric-bone-and-joint-infections-in-french-guiana-a-6-year-retrospective-review
#13
Lindsay Osei, Nawal El Houmami, Philippe Minodier, Anicet Sika, Thierry Basset, Hervé Seligmann, Anne Terraz, Magalie Demar, Jacques Pochard, Jérôme Clouzeau, Pierre-Edouard Fournier, Narcisse Elenga
No abstract text is available yet for this article.
February 15, 2017: Journal of Tropical Pediatrics
https://www.readbyqxmd.com/read/28151084/molecular-detection-of-anaplasma-phagocytophilum-in-wild-cervids-and-hares-in-china
#14
Jifei Yang, Zhijie Liu, Qingli Niu, Jianxun Luo, Xiaolong Wang, Hong Yin
Anaplasma phagocytophilum is an emerging tick-borne pathogen of global veterinary and public health significance. Wild ungulates are suggested to serve as reservoirs for this agent in both the US and Europe. We investigated the occurrence of A. phagocytophilum in sika deer ( Cervus nippon ), Reeves' muntjac ( Muntiacus reevesi ), tufted deer ( Elaphodus cephalophus ), and Chinese hare ( Lepus sinensis ) in China. We detected A. phagocytophilum in nine (53%) free-ranging wild Reeves' muntjac. No positive sample was found in wild tufted deer, hare, or domesticated sika deer...
April 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28128726/the-safety-of-achieved-iron-stores-and-their-effect-on-iv-iron-and-esa-use-post-hoc-results-from-a-randomized-trial-of-ferric-citrate-as-a-phosphate-binder-in-dialysis%C3%A2
#15
RANDOMIZED CONTROLLED TRIAL
Kausik Umanath, Barbara Greco, Diana I Jalal, Molly McFadden, Mohammed Sika, Mark J Koury, Robert Niecestro, Lawrence G Hunsicker, Tom Greene, Julia B Lewis, Jamie P Dwyer
Iron stores assuring optimal efficacy/safety for erythropoiesis are unknown in the dialysis population. Using multicenter trial data, we related safety profiles, erythropoiesis-stimulating agent (ESA), and intravenous iron dosing to achieved iron stores in 441 subjects randomized 2 : 1 to ferric citrate or active control as their phosphate binder over 52 weeks. Intravenous iron was given at each site's discretion if ferritin ≤ 1,000 ng/mL and transferrin saturation ≤ 30%. Multivariable time-dependent Cox regression jointly related the primary safety outcome (composite of cardiac, infection, gastrointestinal, and hepatobiliary serious adverse events) to moving averages of ferritin and transferrin saturation over the preceding 90 days with covariate adjustment...
March 2017: Clinical Nephrology
https://www.readbyqxmd.com/read/28074343/interspecies-sexual-behaviour-between-a-male-japanese-macaque-and-female-sika-deer
#16
Marie Pelé, Alexandre Bonnefoy, Masaki Shimada, Cédric Sueur
Interspecies sexual behaviour or 'reproductive interference' has been reported across a wide range of animal taxa. However, most of these occurrences were observed in phylogenetically close species and were mainly discussed in terms of their effect on fitness, hybridization and species survival. The few cases of heterospecific mating in distant species occurred between animals that were bred and maintained in captivity. Only one scientific study has reported this phenomenon, describing sexual harassment of king penguins by an Antarctic fur seal...
January 10, 2017: Primates; Journal of Primatology
https://www.readbyqxmd.com/read/28071968/rapid-and-robust-authentication-of-deer-antler-velvet-product-by-fast-pcr-rflp-analysis
#17
Chao Jiang, Yan Jin, Xinlei Zhao, Yuan Yuan, Yuyang Zhao, Luqi Huang
Deer antler velvet is widely used as a vitalizing, tonifying, haemopoietic and strengthening agent for debilitated persons in East Asia. To develop a rapid and sensitive method for the identification of the biological source or origin in antler velvet products, a molecular approach was applied using PCR-restriction fragment length polymorphism analysis. The cytochrome b gene sequences of nine cervidae species were analyzed, and a Dde I restriction endonuclease recognition site was found only in sika deer and red deer, the official origin of deer velvet in Chinese pharmacopoeia...
January 10, 2017: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
https://www.readbyqxmd.com/read/28067449/13cra-regulates-the-differentiation-of-antler-chondrocytes-through-targeting-runx3
#18
Hong-Liang Zhang, Hang Cao, Zhan-Qing Yang, Shuang Geng, Kai Wang, Hai-Fan Yu, Bin Guo, Zhan-Peng Yue
Although 13cRA is involved in the regulation of cellular proliferation and differentiation, its physiological roles in chondrocyte proliferation and differentiation still remain unknown. Here, we showed that 13cRA could induce the proliferation of sika deer antler chondrocytes and expression of Ccnd3 and Cdk6. Administration of 13cRA to antler chondrocytes resulted in an obvious increase in the expression of chondrocyte marker Col II and hypertrophic chondrocyte marker Col X. Silencing of Crabp2 expression by specific siRNA could prevent the 13cRA-induced up-regulation of Col X, whereas overexpression of Crabp2 showed the opposite effects...
March 2017: Cell Biology International
https://www.readbyqxmd.com/read/28028480/changes-in-understory-species-occurrence-of-a-secondary-broadleaved-forest-after-mass-mortality-of-oak-trees-under-deer-foraging-pressure
#19
Hiroki Itô
The epidemic of mass mortality of oak trees by Japanese oak wilt has affected secondary deciduous broadleaved forests that have been used as coppices in Japan. The dieback of oak trees formed gaps in the crown that would be expected to enhance the regeneration of shade-intolerant pioneer species. However, foraging by sika deer Cervus nippon has also affected forest vegetation, and the compound effects of both on forest regeneration should be considered when they simultaneously occur. A field study was conducted in Kyôto City, Japan to investigate how these compound effects affected the vegetation of the understory layer of these forests...
2016: PeerJ
https://www.readbyqxmd.com/read/27940503/inhibition-of-nonsense-mediated-rna-decay-by-er-stress
#20
Zhelin Li, John K Vuong, Min Zhang, Cheryl Stork, Sika Zheng
Nonsense-mediated RNA decay (NMD) selectively degrades mutated and aberrantly processed transcripts that contain premature termination codons (PTC). Cellular NMD activity is typically assessed using exogenous PTC-containing reporters. We overcame some inherently problematic aspects of assaying endogenous targets and developed a broadly applicable strategy to reliably and easily monitor changes in cellular NMD activity. Our new method was genetically validated for distinguishing NMD regulation from transcriptional control and alternative splicing regulation, and unexpectedly disclosed a different sensitivity of NMD targets to NMD inhibition...
March 2017: RNA
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