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https://www.readbyqxmd.com/read/29146373/development-and-validation-of-a-fallow-deer-dama-dama-specific-taqman-real-time-pcr-assay-for-the-detection-of-food-adulteration
#1
Maria Kaltenbrunner, Rupert Hochegger, Margit Cichna-Markl
The aim of the present study was to develop a real-time PCR assay for the identification and quantification of fallow deer (Dama dama) in food to detect food adulteration. Despite high sequence homology among different deer species, a fallow deer-specific primer/probe system targeting a fragment of the nuclear MC1-R gene was designed. This primer/probe system did not amplify DNA from 19 other animals and 50 edible plant species. Moderate cross-reactivity was observed for sika deer, red deer, roe deer, reindeer and wild boar...
March 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29115171/sika-pilose-antler-type-i-collagen-promotes-bmsc-differentiation-via-the-erk1-2-and-p38-mapk-signal-pathways
#2
Yanshuang Wang, Su Luo, Dafang Zhang, Xiaobo Qu, Yinfeng Tan
CONTEXT: Sika pilose antler type I collagen (SPC-I) have been reported to promote bone marrow mesenchymal stem cell (BMSC) proliferation and differentiation. However, the underlying mechanism is still unclear. OBJECTIVE: This study investigates the molecular mechanisms of SPC-I on the BMSC proliferation and differentiation of osteoblast (OB) in vitro. MATERIAL AND METHODS: The primary rat BMSC was cultured and exposed to SPC-I at different concentrations (2...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/29065543/the-regulatory-mechanism-of-mlt-mt1-signaling-on-the-growth-of-antler-mesenchymal-cells
#3
Feifei Yang, Changjiu He, Xuyang Sun, Jing Wang, Can Luo, Guoshi Liu, Liguo Yang, Jiajun Xiong, Lijun Huo
Melatonin (MLT) plays an important role in regulating the physiological cycle of seasonal breeding animals. Melatonin receptor I (MT1) is effectively expressed in the cambium layer of deer antler. However, the function and metabolic mechanism of MLT/MT1 signaling in the mesenchymal cells of sika deer remain to be further elucidated. In this work, we detected the effects of MLT/MT1 signaling on mesenchymal cells proliferation and the interaction between MLT/MT1 and IGF1/IGF1-R signaling. The results show that (1) deer antler mesenchymal cells actually express MT1; (2) exogenous melatonin significantly promotes mesenchymal cells proliferation, while MT1 knock-down significantly impairs the positive effects of melatonin; and (3) melatonin significantly enhanced IGF1/IGF1-R signaling, as both the expression of IGF1 and IGF-1R increased, while MT1 knock-down significantly decreased IGF1-R expression and IGF1 synthesis...
October 23, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29064603/histone-deacetylase-inhibitor-stimulates-e2-and-p4-secretion-in-sika-deer-ovarian-granulosa-cells-at-a-moderate-dose
#4
Mingjie Xing, Xiumin Chen, Xiaoxia Li, Yifeng Yang, Xiaoxu Wang, Xinyan Cao, Hailong Xue, Shiyong Wang, Yunfei Diao, Weigang Zhao, Meng Zhao, Xuezhe Cui, Tong Chang, Baozeng Xu, Haijun Wei
The histone deacetylase inhibitor (HDACi) and tumor suppressor play an important role in genome reorganization and epigenetic regulation. In this study, granulosa cells (GCs) isolated from sika deer ovaries were cultured and treated with different concentrations of trichostatin A (TSA) for 48 h. It was found that TSA inhibited GCs proliferation and induced GCs apoptosis by upregulating expression of BAX, meanwhile, downregulating expression of GLUT3, GLUT8, BCL-XL. In addition, TSA caused cell cycle arrest at the G1 and G2/M phase accompanied by reducing expression of Cyclin D2 and CDK4...
October 24, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29038752/seasonal-and-year-round-use-of-the-kushiro-wetland-hokkaido-japan-by-sika-deer-cervus-nippon-yesoensis
#5
Hino Takafumi, Tatsuya Kamii, Takunari Murai, Ryoto Yoshida, Atsuki Sato, Yasuyuki Tachiki, Rika Akamatsu, Tsuyoshi Yoshida
The sika deer (Cervus nippon yesoensis) population in the Ramsar-listed Kushiro Wetland has increased in recent years, and the Ministry of the Environment of Japan has decided to take measures to reduce the impact of deer on the ecosystem. However, seasonal movement patterns of the deer (i.e., when and where the deer inhabit the wetland) remain unclear. We examined the seasonal movement patterns of sika deer in the Kushiro Wetland from 2013 to 2015 by analyzing GPS location data for 28 hinds captured at three sites in the wetland...
2017: PeerJ
https://www.readbyqxmd.com/read/29037227/molecular-detection-of-bartonella-spp-in-deer-ked-lipoptena-cervi-in-poland
#6
Tomasz Szewczyk, Joanna Werszko, Żaneta Steiner-Bogdaszewska, Witold Jeżewski, Zdzisław Laskowski, Grzegorz Karbowiak
BACKGROUND: The bacteria of the genus Bartonella are obligate parasites of vertebrates. Their distribution range covers almost the entire world from America, Europe, Asia to Africa and Australia. Some species of Bartonella are pathogenic for humans. Their main vectors are blood-sucking arthropods such as fleas, ticks and blood-feeding flies. One such dipteran able to transfer vector-borne pathogens is the deer ked (Lipoptena cervi) of the family Hippoboscidae. This species acts as a transmitter of Bartonella spp...
October 16, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29035860/expansion-of-ashworthius-sidemi-in-red-deer-and-roe-deer-from-the-lower-silesian-wilderness-and-its-impact-on-infection-with-other-gastrointestinal-nematodes
#7
Aleksander W Demiaszkiewicz, Dorota Merta, Janusz Kobielski, Katarzyna J Filip, Anna M Pyziel
Nematodes Ashworthius sidemi are typical parasites of Asiatic cervids, mostly sika deer (Cervus nippon). Migrating red deer have probably introduced this parasite to the area of Poland from neighbouring countries. The aim of the study was to determine prevalence and intensity of infection of red deer and roe deer from the Lower Silesian Wilderness with A. sidemi after one year from previous studies and to examine the influence of these parasites on other species of gastrointestinal nematodes. 31 abomasa of red deer and 3 abomasa of roe deer from Forest Dictrict of Ruszów have been examined on the presence of parasites...
December 20, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/29035851/mitochondrial-genotyping-of-fascioloides-magna-from-bavaria-germany
#8
Eva Bazsalovicsová, Ludmila Juhásová, Ivica Králová-Hromadová, Steffen Rehbein
In last few years, a great effort has been made to understand genetic interrelationships of European and North American populations of giant liver fluke Fascioloides magna (Trematoda, Fasciolidae). In Europe, spatial distribution of this parasite is evidently dynamic and ongoing process since new F. magna populations have constantly been emerging. Most recently, occurrence of F. magna in red deer (Cervus elaphus), sika deer (Cervus nippon), fallow deer (Dama dama), roe deer (Capreolus capreolus), and wild boar (Sus scrofa) was reported from north-eastern Bavaria in Germany...
December 20, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/28990924/correction-the-splicing-regulator-ptbp2-controls-a-program-of-embryonic-splicing-required-for-neuronal-maturation
#9
Qin Li, Sika Zheng, Areum Han, Chia-Ho Lin, Peter Stoilov, Xiang-Dong Fu, Douglas L Black
No abstract text is available yet for this article.
October 9, 2017: ELife
https://www.readbyqxmd.com/read/28917308/prevalence-of-pentatrichomonas-hominis-infections-in-six-farmed-wildlife-species-in-jilin-china
#10
Xianhe Li, Jianhua Li, Xichen Zhang, Zhengtao Yang, Ju Yang, Pengtao Gong
Pentatrichomonas hominis is an anaerobic flagellated protozoan that primarily parasitizes the gastrointestinal tract and is a conditional pathogen. It has an extensive host range and is well known as a potential causative agent of zoonotic disease. The objective of this study was to provide the first findings of the prevalence of P. hominis in six farmed wildlife species, sika deer (S.D.), Rex rabbits (R.R.), blue foxes (B.F.), silver foxes (S.F.), raccoon dogs (R.D.) and minks (M.), that are commercially important in Jilin Province, China...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28876448/engaging-postgraduate-students-undertaking-clinical-pharmacology-using-gosoapbox-for-problem-based-learning
#11
Dianne Sika-Paotonu, Brian Robinson, Tim Maling
No abstract text is available yet for this article.
September 1, 2017: Journal of Nursing Education
https://www.readbyqxmd.com/read/28869947/the-protective-effects-of-sika-deer-antler-protein-on-cisplatin-induced-nephrotoxicity
#12
Huihai Yang, Wei Li, Lulu Wang, Wenqing Li, Hang Sun, Xiaofeng He, Jing Zhang
BACKGROUND/AIMS: This study measured the effect of Sika deer (Cervus nippon Temminck) antler protein (SDAPR), glycoproteins (SDAG), and polysaccharides (SDAPO) on cisplatin-induced cytotoxicity in HEK 293 cells, and investigated the effect of SDAPR against cisplatin-induced nephrotoxicity in mice. METHODS: Cell viability was measured by MTT assay. ICR mice were randomly divided into five groups: control, cisplatin with vehicle, and cisplatin with SDAPR at three concentrations: 5, 10, or 20 mg/kg, p...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28823128/molecular-cloning-and-expression-analysis-of-annexin-a2-gene-in-sika-deer-antler-tip
#13
Yanling Xia, Haomiao Qu, Binshan Lu, Qiang Zhang, Heping Li
Objective: Molecular cloning and bioinformatics analysis of Annexin A2 gene in sika deer antler tip were conducted. The role of Annexin A2 gene in the growth and development of the antler were analyzed initially. Methods: The reverse transcriptase-polymerase chain reaction (RT-PCR) was used to clone the cDNA sequence of the Anxa2 gene from antler tip of sika deer (Cervus nippon hortulorum) and the bioinformatics tools provided on the web site were applied to analyze the amino acid sequence of Anxa2 protein...
August 16, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28766300/a-cell-based-high-throughput-method-for-identifying-modulators-of-alternative-splicing
#14
Sika Zheng
Alternative splicing, a key regulatory process of gene expression, is controlled by trans-acting factors that recognize cis-elements in premature RNA transcripts to affect spliceosome assembly and splice site choices. Extracellular stimuli and signaling cascades can converge on RNA binding splicing regulators to affect alternative splicing. Defects in splicing regulation have been associated with various human diseases, and modification of disease-causing splicing events presents great therapeutic promise. Determining splicing regulators and/or upstream modulators has been difficult with low throughput, low sensitivity, and low specificity...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28765719/time-series-data-of-a-broadleaved-secondary-forest-in-japan-as-affected-by-deer-and-mass-mortality-of-oak-trees
#15
Hiroki Itô
BACKGROUND: Abandonment of broadleaved secondary forests that have been used for various purposes may cause the loss of biodiversity. Some of these forests suffer from diseases such as Japanese oak wilt. An increasing number of deer also impact some of them. Monitoring and recording the status of such forests is important for their proper management. NEW INFORMATION: This data set provides a concrete example of temporal changes in a temperate broadleaved secondary forest...
2017: Biodiversity Data Journal
https://www.readbyqxmd.com/read/28758428/the-proteins-from-sika-deer-antler-as-potential-modulators-on-cisplatin-induced-cytotoxicity-in-human-embryonic-kidney-293-cells
#16
Huihai Yang, Wei Li, Lulu Wang, Xiaofeng He, Hang Sun, Jing Zhang
Our study aimed to investigate the protective role of SDAPR on cisplatin-induced cytotoxicity and its' possible mechanism in HEK293 cells. Cell viability was measured by MTT assay. Oxidative stress (SOD, GSH, LDH and MDA), inflammatory factors (TNF-α and IL-6) and apoptosis-related proteins (caspase-3, Bax, Bcl-2) expression were measured. The apoptotic cells were observed by TUNEL staining. Our study results indicated that non-cytotoxic levels of SDAPR significantly increased viability rate (LD50 value of cisplatin is 20 μM), which improved antioxidant defence, attenuated apoptosis by decreasing expression levels of cleaved-caspase-3 and Bax, increasing Bcl-2 expression and inhibiting apoptotic positive cells in HEK 293 cells...
July 31, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28751500/genome-wide-snp-discovery-and-analysis-of-genetic-diversity-in-farmed-sika-deer-cervus-nippon-in-northeast-china-using-double-digest-restriction-site-associated-dna-sequencing
#17
Hengxing Ba, Boyin Jia, Guiwu Wang, Yifeng Yang, Gilead Kedem, Chunyi Li
Sika deer are an economically valuable species owing to their use in traditional Chinese medicine, particularly their velvet antlers. Sika deer in northeast China are mostly farmed in enclosure. Therefore, genetic management of farmed sika deer would benefit from detailed knowledge of their genetic diversity. In this study, we generated over 1.45 billion high-quality paired-end reads (288 Gbp) across 42 unrelated individuals using double-digest restriction site-associated DNA sequencing (ddRAD-seq). A total of 96,188 (29...
September 7, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28749086/1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol-plag-reduces-hepatic-injury-in-concanavalin-a-treated-mice
#18
Young-Eun Ko, Sun Young Yoon, Sun-Yung Ly, Joo-Heon Kim, Ki-Young Sohn, Jae Wha Kim
1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (PLAG), a chemically synthesized monoacetyldiaglyceride, is one of the constituents in Sika deer antlers and has been known traditionally as having immunomodulatory effects. However, the mechanism by which PLAG controls neutrophil migration, which evokes liver injury in the hepatitis animal model, remains largely unknown. This study was designed to evaluate the immunomodulatory effects of PLAG on cytokine secretion and neutrophil migration in vivo and in vitro. The injection of mice with Concanavalin A (Con A) induced leukocyte infiltration in the liver and increased plasma cytokine levels...
July 27, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28744165/asymptomatic-multinodular-splenoma-splenic-hamartoma-in-a-child-with-sickle-cell-anemia
#19
Narcisse Elenga, Sylvain Labbé, Nicolas Leduc, Anicet Sika, Emma Cuadro, Laurence Long, Falucar Njuieyon, Rémi Kom-Tchameni, Thierry Basset
Splenoma is a rare and benign malformation usually fortuitously diagnosed during imaging, surgery or, unfortunately, at autopsy. Although splenoma was first described in 1861, its association with hematological pathology is a very unusual condition in children. We report the case of an asymptomatic splenoma in an 8-year-old boy with sickle cell anemia, whose diagnosis was confirmed after conventional splenectomy.
2017: International Medical Case Reports Journal
https://www.readbyqxmd.com/read/28735469/phylogenetic-relationships-between-dicrocoelium-chinensis-populations-in-japan-and-china-based-on-mitochondrial-nad1-gene-sequences
#20
Kei Hayashi, WenQiang Tang, Yuma Ohari, Maiko Ohtori, Uday Kumar Mohanta, Kayoko Matsuo, Hiroshi Sato, Tadashi Itagaki
We carried out phylogenetic analyses of the relationships between Dicrocoelium chinensis populations in Japan and China using molecular markers. One hundred nine lancet flukes collected from Japan and China were identified as D. chinensis based on their testis orientation and the nucleotide sequences of their ribosomal ITS2. These flukes were analyzed phylogenetically using mitochondrial nad1 gene sequences. An analysis of molecular variance found that the percentage of variation between the countries was extremely high, indicating that the D...
July 22, 2017: Parasitology Research
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