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Ex vivo perfusion

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https://www.readbyqxmd.com/read/28922300/recent-advances-in-the-field-of-warm-ex-vivo-liver-perfusion
#1
Dagmar Kollmann, Markus Selzner
PURPOSE OF REVIEW: Organ shortage remains a major obstacle for liver transplantation, resulting in an increased mortality on the liver transplant waiting list. The usage of extended criteria donors (ECD) is a strategy to increase the number of available donor organs, however, with the risk of a higher rate of posttransplant graft dysfunction. Novel preservation strategies, such as warm ex-vivo liver perfusion, could improve the outcome of liver transplantation with ECD grafts. The present review summarizes the advances in the field of warm ex-vivo liver perfusion over the last 12 months...
September 16, 2017: Current Opinion in Organ Transplantation
https://www.readbyqxmd.com/read/28922117/magnetic-resonance-mediated-radiofrequency-ablation
#2
Yik-Kiong Hue, Alexander R Guimaraes, Ouri Cohen, Erez Nevo, Abraham Roth, Jerome L Ackerman, Jerome L Ackerman
OBJECTIVE: To introduce magnetic resonance mediated radiofrequency ablation (MR-RFA), in which the MRI scanner uniquely serves both diagnostic and therapeutic roles. In MR-RFA scanner-induced RF heating is channeled to the ablation site via a Larmor frequency RF pickup device and needle system, and controlled via the pulse sequence. METHODS: MR-RFA was evaluated with simulation of electric and magnetic fields to predict the increase in local specific-absorption-rate (SAR)...
September 18, 2017: IEEE Transactions on Medical Imaging
https://www.readbyqxmd.com/read/28916504/going-skin-deep-a-direct-comparison-of-penetration-potential-of-lipid-based-nanovesicles-on-the-isolated-perfused-human-skin-flap-model
#3
Selenia Ternullo, Louis de Weerd, Ann Mari Holsæter, Gøril Eide Flaten, Nataša Škalko-Basnet
Phospholipid-based nanocarriers are attractive drug carriers for improved local skin therapy. In the present study, the recently developed isolated perfused human skin flap (IPHSF) model was used to directly compare the skin penetration enhancing potential of the three commonly used nanocarriers, namely conventional liposomes (CLs), deformable liposomes (DLs) and solid lipid nanoparticles (SLNs). Two fluorescent markers, calcein (hydrophilic) or rhodamine (lipophilic), were incorporated individually in the three nanosystems...
September 12, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28905773/re-continuous-normothermic-ex%C3%A2-vivo-kidney-perfusion-is-superior-to-brief-normothermic-perfusion-following-static-cold-storage-in-donation-after-circulatory-death-pig-kidney-transplantation
#4
https://www.readbyqxmd.com/read/28901972/normothermic-machine-perfusion-of-the-kidney
#5
Annemarie Weissenbacher, James Hunter
PURPOSE OF REVIEW: Normothermic machine perfusion (NMP) is a preservation method that is generating increasing interest. The aim of this review is to summarise the current status of NMP in regards of kidney viability assessment, reducing organ damage and improving transplant logistics. RECENT FINDINGS: The results of recent large animal experiments and clinical trials show that continuous prolonged normothermic ex-vivo kidney perfusion appears better than a brief period of NMP after static cold storage in terms of renal injury and function...
September 8, 2017: Current Opinion in Organ Transplantation
https://www.readbyqxmd.com/read/28898758/modular-flow-chamber-for-engineering-bone-marrow-architecture-and-function
#6
Christian A Di Buduo, Paolo M Soprano, Lorenzo Tozzi, Stefania Marconi, Ferdinando Auricchio, David L Kaplan, Alessandra Balduini
The bone marrow is a soft, spongy, gelatinous tissue found in the hollow cavities of flat and long bones that support hematopoiesis in order to maintain the physiologic turnover of all blood cells. Silk fibroin, derived from Bombyx mori silkworm cocoons, is a promising biomaterial for bone marrow engineering, because of its tunable architecture and mechanical properties, the capacity of incorporating labile compounds without loss of bioactivity and demonstrated ability to support blood cell formation. In this study, we developed a bone marrow scaffold consisting of a modular flow chamber made of polydimethylsiloxane, holding a silk sponge, prepared with salt leaching methods and functionalized with extracellular matrix components...
August 8, 2017: Biomaterials
https://www.readbyqxmd.com/read/28895380/how-to-optimize-the-lung-donor
#7
Gabriele Sales, Andrea Costamagna, Vito Fanelli, Massimo Boffini, Francesco Pugliese, Luciana Mascia, Luca Brazzi
In the last two decades, lung transplantation emerged as the standard of care for patients with advanced and terminal lung disease. Despite the increment in lung transplantation rates, in 2016 the overall mortality while on waiting list in Italy reached the 10%, whereas only the 39% of the wait-list patients were successfully transplanted. A number of approaches, including protective ventilatory strategy, accurate management of fluid balance, administration of a hormonal resuscitation therapy have been reported to improve lung donor performance before organ retrieval...
September 11, 2017: Minerva Anestesiologica
https://www.readbyqxmd.com/read/28893911/molecular-and-functional-characterization-of-the-endothelial-atp-sensitive-potassium-channel
#8
Qadeer Aziz, Yiwen Li, Naomi Anderson, Leona Ojake, Elena Tsisanova, Andrew Tinker
ATP-sensitive potassium (KATP) channels are widely expressed in the cardiovascular system where they regulate a range of biological activities by linking cellular metabolism with membrane excitability. KATP channels in vascular smooth muscle have a well-defined role in regulating vascular tone. KATP channels are also thought to be expressed in vascular endothelial cells (ECs), but their presence and function in this context is less clear. As a result, we aimed to investigate the molecular composition and physiological role of endothelial KATP channels...
September 11, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28892871/formulation-by-design-approach-for-development-of-ultrafine-self-nanoemulsifying-systems-of-rosuvastatin-calcium-containing-long-chain-lipophiles-for-hyperlipidemia-management
#9
Sarwar Beg, O P Katare, Bhupinder Singh
The present work entails systematic development of liquid self-nanoemulsifying drug delivery systems (L-SNEDDS) of rosuvastatin calcium containing long-chain lipophiles using QbD-driven Formulation by Design (FbD) approach. Elements of quality target product profile (QTPP) were defined and critical material attributes (CQAs) earmarked. Excipient screening was performed for selecting a suitable long-chain lipophile along with a surfactant and a cosolvent. Maximal drug solubility was observed in Peceol (i.e., lipid), Tween 80 (i...
August 31, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28889600/normothermic-ex-vivo-kidney-perfusion-for-graft-quality-assessment-prior-to-transplantation
#10
J Moritz Kaths, Mátyás Hamar, Juan Echeverri, Ivan Linares, Peter Urbanellis, Jun Yu Cen, Sujani Ganesh, Luke S Dingwell, Paul Yip, Rohan John, Darius Bagli, Istvan Mucsi, Anand Ghanekar, David Grant, Lisa A Robinson, Markus Selzner
Normothermic ex vivo kidney perfusion (NEVKP) represents a novel approach for graft preservation and functional improvement in kidney transplantation. We investigated whether NEVKP also allows graft quality assessment prior to transplantation. Kidneys from 30kg pigs were recovered in a model of heart-beating donation (HBD) (group A), following 30min of warm ischemia (WI) (group B), or 60min WI (group C) (n=5/group). Following 8hrs of NEVKP, contralateral kidneys were resected, grafts autotransplanted, and pigs followed for 3 days...
September 10, 2017: American Journal of Transplantation
https://www.readbyqxmd.com/read/28887099/enhancing-biopharmaceutical-performance-of-an-anticancer-drug-by-long-chain-pufa-based-self-nanoemulsifying-lipidic-nanomicellar-system
#11
Rajneet Kaur Khurana, Sarwar Beg, Andrea Julie Burrow, Rakesh K Vashishta, O P Katare, Satvinder Kaur, Prashant Kesherwani, Kamalinder K Singh, Bhupinder Singh
The aim of this study was to develop polyunsaturated fatty acid (PUFA) long chain glyceride (LCG) enriched self-nanoemulsifying lipidic nanomicelles systems (SNELS) for augmenting lymphatic uptake and enhancing oral bioavailability of docetaxel and compare its biopharmaceutical performance with a medium-chain fatty acid glyceride (MCG) SNELS. Equilibrium solubility and pseudo ternary phase studies facilitated the selection of suitable LCG and MCG. The critical material attributes (CMAs) and critical process parameters (CPPs) were earmarked using Placket-Burman Design (PBD) and Fractional Factorial Design (FFD) for LCG- and MCG-SNELS respectively, and nano micelles were subsequently optimized using I- and D-optimal designs...
September 5, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28874022/ex-vivo-administration-of-trimetazidine-improves-post-transplant-lung-function-in-pig-model
#12
Tugba Cosgun, Ilker Iskender, Yoshito Yamada, Stephan Arni, Miriam Lipiski, Koen van Tilburg, Walter Weder, Ilhan Inci
OBJECTIVES: Ex vivo lung perfusion (EVLP) is not only used to assess marginal donor lungs but is also used as a platform to deliver therapeutic agents outside the body. We previously showed the beneficial effects of trimetazidine (TMZ) on ischaemia reperfusion (IR) injury in a rat model. This study evaluated the effects of TMZ in a pig EVLP transplant model. METHODS: Pig lungs were retrieved and stored for 24 h at 4°C, followed by 4 h of EVLP. Allografts were randomly allocated to 2 groups ( n  = 5 each)...
July 1, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28872471/lung-xenotransplantation
#13
Hisashi Sahara, Hironosuke Watanabe, Thomas Pomposelli, Kazuhiko Yamada
PURPOSE OF REVIEW: This review describes the most recent progress in xeno lung transplantation (XLTx) to date. It describes the potential mechanisms of early xeno lung graft loss, as well as the latest therapeutic strategies to overcome them. RECENT FINDINGS: Using ex-vivo perfusion models of porcine lungs with human blood, the use of genetically modified pig lungs along with novel pharmaceutical approaches has recently been studied. Strategies that have demonstrated improved lung survival include the knockout of known xenoantigens (GalTKO and N-glycolylneuraminic acid-KO), genes that regulate complement activation (hCD46 and hCD55), as well as the inflammation/coagulation cascade (human leukocyte antigen-E, human thrombomodulin, human endothelial protein C receptor, hCD47, hCD39, hCD73 and heme oxygenase-1)...
September 1, 2017: Current Opinion in Organ Transplantation
https://www.readbyqxmd.com/read/28871615/controlled-oxygenated-rewarming-up-to-normothermia-for-pre-transplant-reconditioning-of-liver-grafts
#14
Charlotte von Horn, Hideo Baba, Patrik Hannaert, Thierry Hauet, Henri Leuvenink, Andreas Paul, Thomas Minor
Controlled oxygenated rewarming (COR) up to 20°C during ex vivo machine perfusion limits reperfusion induced tissue injury upon graft implantation. Rewarming up to normothermia might add further benefits and provide better prediction of post transplantation organ function. The effect of 90 min of oxygenated machine perfusion with Aqix RS-I after cold storage combined with gentle rewarming up to 20°C (COR20) or 35°C (COR35) was studied in rat livers and compared with cold storage alone (CS, n=6, resp). Post-preservation recovery was evaluated upon warm reperfusion using an established in vitro system...
September 4, 2017: Clinical Transplantation
https://www.readbyqxmd.com/read/28870398/selective-lobar-exclusion-by-venous-clamping-during-ex%C3%A2-vivo-lung-perfusion
#15
Thomas Schweiger, Alberto Benazzo, Gyoergy Lang, José Ramon Matilla, Walter Klepetko
No abstract text is available yet for this article.
June 15, 2017: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28864020/pre-clinical-evaluation-of-a-nanoparticle-based-blood-pool-contrast-agent-for-mr-imaging-of-the-placenta
#16
Ketan B Ghaghada, Zbigniew A Starosolski, Saakshi Bhayana, Igor Stupin, Chandreshkumar V Patel, Rohan C Bhavane, Haijun Gao, Andrey Bednov, Chandrasekhar Yallampalli, Michael Belfort, Verghese George, Ananth V Annapragada
INTRODUCTION: Non-invasive 3D imaging that enables clear visualization of placental margins is of interest in the accurate diagnosis of placental pathologies. This study investigated if contrast-enhanced MRI performed using a liposomal gadolinium blood-pool contrast agent (liposomal-Gd) enables clear visualization of the placental margins and the placental-myometrial interface (retroplacental space). Non-contrast MRI and contrast-enhanced MRI using a clinically approved conventional contrast agent were used as comparators...
September 2017: Placenta
https://www.readbyqxmd.com/read/28862969/three-dimensional-integrated-functional-structural-and-computational-mapping-to-define-the-structural-fingerprints-of-heart-specific-atrial-fibrillation-drivers-in-human-heart-ex-vivo
#17
Jichao Zhao, Brian J Hansen, Yufeng Wang, Thomas A Csepe, Lidiya V Sul, Alan Tang, Yiming Yuan, Ning Li, Anna Bratasz, Kimerly A Powell, Ahmet Kilic, Peter J Mohler, Paul M L Janssen, Raul Weiss, Orlando P Simonetti, John D Hummel, Vadim V Fedorov
BACKGROUND: Structural remodeling of human atria plays a key role in sustaining atrial fibrillation (AF), but insufficient quantitative analysis of human atrial structure impedes the treatment of AF. We aimed to develop a novel 3-dimensional (3D) structural and computational simulation analysis tool that could reveal the structural contributors to human reentrant AF drivers. METHODS AND RESULTS: High-resolution panoramic epicardial optical mapping of the coronary-perfused explanted intact human atria (63-year-old woman, chronic hypertension, heart weight 608 g) was conducted during sinus rhythm and sustained AF maintained by spatially stable reentrant AF drivers in the left and right atrium...
August 22, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28859848/chronic-in-vivo-nitric-oxide-deficiency-impairs-cardiac-functional-recovery-after-ischemia-in-female-but-not-male-mice
#18
Laura A Bienvenu, James Morgan, Melissa E Reichelt, Lea M D Delbridge, Morag J Young
Nitric oxide (NO) is an important regulator of cardiac function and plays a key role in ischemic cardioprotection. The role of chronic NO deficiency in coordinating ischemic vulnerability in female myocardium has not been established. The aim of this study was to determine the influence of chronic in vivo NO synthase inhibition in modulating ex vivo ischemia-reperfusion responses in female hearts (relative to males). Mice were subjected to l-NAME (l-N(G)-Nitroarginine-methyl-ester) treatment in vivo for 8weeks...
August 30, 2017: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/28857775/ex-vivo-lung-perfusion-past-present-and-future
#19
Hannah C D'Cunha, Mauricio Rojas
Lung transplant is the only therapy for several end-stage lung diseases; however, the number of donated lungs suitable for transplant is not sufficient, and hundreds of patients have died waiting for an organ. Ex vivo lung perfusion (EVLP) has emerged as a new technology with the potential of reconditioning human donor lungs previously unsuitable for transplantation. Since the first successful transplant of a lung treated using EVLP in the year 2000, multiple clinical trials have demonstrated, in several transplant centers around the word, the feasibility and the potential of EVLP to increase the total number of lungs available for transplant...
August 29, 2017: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/28856718/tomoelastography-of-the-native-kidney-regional-variation-and-physiological-effects-on-in-vivo-renal-stiffness
#20
Stephan Rodrigo Marticorena Garcia, Markus Grossmann, Sophia Theresa Lang, Heiko Tzschätzsch, Florian Dittmann, Bernd Hamm, Jürgen Braun, Jing Guo, Ingolf Sack
PURPOSE: To measure normal renal stiffness in adults, taking into account regional variation, hydration, and urinary status. METHODS: Thirty-six healthy volunteers were examined by tomoelastography based on MR elastography at four frequencies, from 40 to 70 Hz and multifrequency shear wave speed recovery. Regional wave speeds were derived for the medulla, cortex (inner cortex and outer cortex), and renal pelvis, and examined for age-related effects. Subgroups were repeatedly examined for reproducibility, amount of prior water drinking, and urinary status...
August 30, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
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