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Ex vivo perfusion

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https://www.readbyqxmd.com/read/29771714/remifentanil-induces-cardio-protection-against-ischemia-reperfusion-injury-by-inhibiting-endoplasmic-reticulum-stress-through-the-maintenance-of-zinc-homeostasis
#1
Mingwei Sheng, Ge Zhang, Jiannan Wang, Qing Yang, Huanhuan Zhao, Xinxin Cheng, Zhelong Xu
BACKGROUND: Although it is well known that remifentanil (Rem) elicits cardiac protection against ischemia/reperfusion (I/R) injury, the underlying mechanism remains unclear. This study tested if Rem can protect the heart from I/R injury by inhibiting endoplasmic reticulum (ER) stress through the maintenance of zinc (Zn) homeostasis. METHODS: Isolated rat hearts were subjected to 30 minutes of regional ischemia followed by 2 hours of reperfusion. Rem was given by 3 consecutive 5-minute infusions, and each infusion was followed by a 5-minute drug-free perfusion before ischemia...
May 15, 2018: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/29765507/ex-vivo-cardiotoxicity-of-antineoplastic-casiopeinas-is-mediated-through-energetic-dysfunction-and-triggered-mitochondrial-dependent-apoptosis
#2
Christian Silva-Platas, César A Villegas, Yuriana Oropeza-Almazán, Mariana Carrancá, Alejandro Torres-Quintanilla, Omar Lozano, Javier Valero-Elizondo, Elena C Castillo, Judith Bernal-Ramírez, Evaristo Fernández-Sada, Luis F Vega, Niria Treviño-Saldaña, Héctor Chapoy-Villanueva, Lena Ruiz-Azuara, Carmen Hernández-Brenes, Leticia Elizondo-Montemayor, Carlos E Guerrero-Beltrán, Karla Carvajal, María E Bravo-Gómez, Gerardo García-Rivas
Casiopeinas are a group of copper-based antineoplastic molecules designed as a less toxic and more therapeutic alternative to cisplatin or Doxorubicin; however, there is scarce evidence about their toxic effects on the whole heart and cardiomyocytes. Given this, rat hearts were perfused with Casiopeinas or Doxorubicin and the effects on mechanical performance, energetics, and mitochondrial function were measured. As well, the effects of Casiopeinas-triggered cell death were explored in isolated cardiomyocytes...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29754046/brain-stiffens-post-mortem
#3
J Weickenmeier, M Kurt, E Ozkaya, R de Rooij, T C Ovaert, R L Ehman, K Butts Pauly, E Kuhl
Alterations in brain rheology are increasingly recognized as a diagnostic marker for various neurological conditions. Magnetic resonance elastography now allows us to assess brain rheology repeatably, reproducibly, and non-invasively in vivo. Recent elastography studies suggest that brain stiffness decreases one percent per year during normal aging, and is significantly reduced in Alzheimer's disease and multiple sclerosis. While existing studies successfully compare brain stiffnesses across different populations, they fail to provide insight into changes within the same brain...
April 22, 2018: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/29740711/new-avenues-for-nanoparticle-related-therapies
#4
Michael Zhao, Mingyao Liu
Development of nanoparticle-based drug delivery systems has been attempted for the treatment of cancer over the past decade. The enhanced permeability and retention (EPR) effect is the major mechanism to passively deliver nanodrugs to tumor tissue. However, a recent systematic review demonstrated limited success of these studies, with the clearance of nanoparticles by the mononuclear phagocytic system (MPS) being a major hurdle. Herein, we propose that nanotechnologists should reconsider their research focuses, aiming for therapeutic targets other than cancer...
May 8, 2018: Nanoscale Research Letters
https://www.readbyqxmd.com/read/29735634/evaluation-of-sildenafil-and-tadalafil-for-reversing-constriction-of-fetal-arteries-in-a-human-placenta-perfusion-model
#5
Robert B Walton, Luckey C Reed, Sarah M Estrada, Stacey S Schmiedecke, Diana L Villazana-Kretzer, Peter G Napolitano, Nicholas Ieronimakis
Fetal growth restriction resulting from reduced placental blood perfusion is a major cause of neonatal morbidity and mortality. Aside from intense surveillance and early delivery, there is no treatment for fetal growth restriction. A potential treatment associated with placental vasoconstriction is the class of PDE5 (phosphodiesterase type 5) inhibitors such as sildenafil, which is known to cross the placenta. In contrast, tadalafil, a more potent and selective PDE5 inhibitor has not been studied in pregnancy or experimental models of fetal growth restriction...
May 7, 2018: Hypertension
https://www.readbyqxmd.com/read/29730077/ex-vivo-normothermic-porcine-pancreas-a-physiological-model-for-preservation-and-transplant-study
#6
Rohan Kumar, Wen Yuan Chung, Franscois Runau, John David Isherwood, Kean Guan Kuan, Kevin West, Giuseppe Garcea, Ashley Robert Dennison
INTRODUCTION: An ex vivo normothermic porcine pancreas perfusion (ENPPP) model was established to investigate effects of machine perfusion pressures on graft preservation. METHODOLOGY: Nine porcine pancreata were perfused with autologous blood at 50 mmHg (control) pressure. Graft viability was compared against four ex-vivo porcine pancreata perfused at 20 mmHg ('low') pressure. Arterio-venous oxygen gas differentials, biochemistry, and graft insulin responses to glucose stimulation were compared...
May 3, 2018: International Journal of Surgery
https://www.readbyqxmd.com/read/29723064/cell-free-hemoglobin-in-the-fetoplacental-circulation-a-novel-cause-of-fetal-growth-restriction
#7
Adam Brook, Annie Hoaksey, Rekha Gurung, Edward E C Yoong, Rosanna Sneyd, Georgia C Baynes, Helen Bischof, Sarah Jones, Lucy E Higgins, Carolyn Jones, Susan L Greenwood, Rebecca L Jones, Magnus Gram, Ingrid Lang, Gernot Desoye, Jenny Myers, Henning Schneider, Stefan R Hansson, Ian P Crocker, Paul Brownbill
Cell free hemoglobin impairs vascular function and blood flow in adult cardiovascular disease. In this study, we investigated the hypothesis that free fetal hemoglobin (fHbF) compromises vascular integrity and function in the fetoplacental circulation, contributing to the increased vascular resistance associated with fetal growth restriction (FGR). Women with normal and FGR pregnancies were recruited and their placentas collected freshly postpartum. FGR fetal capillaries showed evidence of erythrocyte vascular packing and extravasation...
May 3, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29722035/time-and-temperature-dependence-of-radiofrequency-ablation-in-the-human-placenta
#8
Nisarat Phithakwatchara, Preechaya Makarasen, Katika Nawapun, Suchanan Hanamornroongruang, Sommai Viboonchart, Tuangsit Wataganara
OBJECTIVE: To compare radiofrequency (RF) effects on fresh placentae with varying levels of sustained time (Ts) and degrees of target temperature (o t). METHOD: A total of 108 pieces of fresh placentae were coagulated with a 2-cm RF needle at 60 W in an organ bath. The vertical and horizontal diameters (Vd, Hd) of tissue coagulation visualized by ultrasound were measured. The impacts of 12 different Ts-o t combinations on the ablation size ascertained on pathological examination (Vdp , Hdp ) were compared using two-way ANOVA...
May 2, 2018: Prenatal Diagnosis
https://www.readbyqxmd.com/read/29721067/3d-imaging-and-quantitative-analysis-of-vascular-networks-a-comparison-of-ultramicroscopy-and-micro-computed-tomography
#9
Jeremy Epah, Katalin Pálfi, Franziska Luise Dienst, Pedro Felipe Malacarne, Rolf Bremer, Michael Salamon, Sandeep Kumar, Hanjoong Jo, Christoph Schürmann, Ralf Peter Brandes
RATIONALE: Classic histology is the gold standard for vascular network imaging and analysis. The method however is laborious and prone to artefacts. Here, the suitability of ultramicroscopy (UM) and micro-computed tomography (CT) was studied to establish potential alternatives to histology. METHODS: The vasculature of murine organs (kidney, heart and atherosclerotic carotid arteries) was visualized using conventional 2D microscopy, 3D light sheet ultramicroscopy (UM) and micro-CT...
2018: Theranostics
https://www.readbyqxmd.com/read/29718705/influence-of-ex-vivo-perfusion-on-the-biomolecular-profile-of-rat-lungs
#10
Caterina Lonati, Giulia A Bassani, Daniela Brambilla, Patrizia Leonardi, Andrea Carlin, Alice Faversani, Stefano Gatti, Franco Valenza
Despite increasing clinical adoption, biologic influences of ex vivo lung perfusion (EVLP) remain insufficiently elucidated. The aim of the current study was to investigate biomolecular changes induced by EVLP in rat lungs. EVLP was maintained for 180 min. Hyaluronan, mediators, and cells were assessed in the perfusate. Gene expression, signaling pathways, and ATP content were investigated in lung tissue. EVLP induced the release of medium-high molecular weight hyaluronan and transcription of hyaluronan synthases ( P < 0...
May 2, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29710813/decellularized-diaphragmatic-muscle-drives-a-constructive-angiogenic-response-in-vivo
#11
Mario Enrique Alvarèz Fallas, Martina Piccoli, Chiara Franzin, Alberto Sgrò, Arben Dedja, Luca Urbani, Enrica Bertin, Caterina Trevisan, Piergiorgio Gamba, Alan J Burns, Paolo De Coppi, Michela Pozzobon
Skeletal muscle tissue engineering (TE) aims to efficiently repair large congenital and acquired defects. Biological acellular scaffolds are considered a good tool for TE, as decellularization allows structural preservation of tissue extracellular matrix (ECM) and conservation of its unique cytokine reservoir and the ability to support angiogenesis, cell viability, and proliferation. This represents a major advantage compared to synthetic scaffolds, which can acquire these features only after modification and show limited biocompatibility...
April 28, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29706428/functional-ct-assessment-of-extravascular-contrast-distribution-volume-and-myocardial-perfusion-in-acute-myocardial-infarction
#12
Aaron So, Gerald Wisenberg, Patrick Teefy, Andrew Yadegari, Rodrigo Bagur, Jennifer Hadway, Laura Morrison, Anna MacDonald, Dave Gaskin, John Butler, Heather Biernaski, Stephanie Skanes, Stella DohYeoun Park, Ali Islam, Jiang Hsieh, Ting-Yim Lee
PURPOSE: In a pig model of acute myocardial infarction (AMI), we validated a functional computed tomography (CT) technique for concomitant assessment of myocardial edema and ischemia through extravscualar contrast distribution volume (ECDV) and myocardial perfusion (MP) measurements from a single dynamic imaging session using a single contrast bolus injection. METHODS: In seven pigs, balloon catheter was used to occlude the distal left anterior descending artery for one hour followed by reperfusion...
April 26, 2018: International Journal of Cardiology
https://www.readbyqxmd.com/read/29705279/effects-of-subnormothermic-perfusion-before-transplantation-for-liver-grafts-from-donation-after-cardiac-death-a-simplified-dripping-perfusion-method-in-pigs
#13
Y Kakizaki, S Miyagi, K Shimizu, H Kumata, M Matsumura, Y Miyazaki, K Fukuoka, S S Uematsu, K Tokodai, Y Hara, C Nakanishi, M Unno, T Kamei, M Goto
BACKGROUND: Liver transplantation from donors after cardiac death (DCD) provides a solution to the donor shortage. However, DCD liver grafts are associated with a high incidence of primary graft nonfunction. We investigated the effectiveness of subnormothermic porcine liver perfusion, before transplantation from DCD, on graft viability. METHODS: Landrace pigs (25-30 kg) were randomly allocated to 3 groups (5 per group): heart-beating (HB) graft, transplanted after a 4-hour period of cold storage (CS); DCD graft, retrieved 20 minutes after apnea-induced cardiac arrest (respiratory withdrawal) and transplanted after a 4-hour period of CS; and subnormothermic ex vivo liver perfusion (SELP) graft, retrieved in the same manner as the DCD graft but perfused with a subnormothermic oxygenated Krebs-Henseleit buffer (21-25°C, 10-15 cm H2 O) for 30 minutes in a simplified dripping manner, without a machine perfusion system, after the 4-hour period of CS, and subsequently transplanted...
April 25, 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/29702648/development-of-a-cardiac-loading-device-to-monitor-cardiac-function-during-ex-vivo-graft-perfusion
#14
Emilie Farine, Manuel U Egle, Alice C Boone, Sandro Christensen, Thierry P Carrel, Hendrik T Tevaearai Stahel, Sarah L Longnus
BACKGROUND: Ex vivo heart perfusion systems, allowing continuous perfusion of the coronary vasculature, have recently been introduced to limit ischemic time of donor hearts prior to transplantation. Hearts are, however, perfused in an unloaded manner (via the aorta) and therefore, cardiac contractile function cannot be reliably evaluated. OBJECTIVES: We aim to develop a ventricular loading device that enables monitoring of myocardial function in an ex vivo perfusion system...
2018: PloS One
https://www.readbyqxmd.com/read/29702553/cytokine-and-chemokine-recovery-is-increased-by-colloid-perfusates-during-dermal-microdialysis
#15
Sven R Quist, Claudia Kirbs, Charlotte Kloft, Harald P Gollnick
Cytokines and chemokines play important roles in cell signalling, and microdialysis is a promising tool for monitoring these inflammation markers ex vivo. Therefore, the collecting of these mediators at the highest concentrations possible is crucial. Depending on the size of the mediator of interest, the collection of these high molecular mass molecules has thus far been difficult due to their low recovery, even when using high cut-off (100 kDa) microdialysis membranes. This study aimed to optimize the recovery of various cytokines and chemokines by validating the use of different perfusates in cutaneous microdialysis, and comparing intravenous (i...
April 27, 2018: Materials
https://www.readbyqxmd.com/read/29702250/type-2-diabetes-impairs-angiogenesis-and-osteogenesis-in-calvarial-defects-microct-study-in-zdf-rats
#16
Guavri Caliaperoumal, Maite Souyet, Morad Bensidhoum, Herve Petite, Fani Anagnostou
OBJECTIVES: The present study was motivated by the fact that bone regeneration in the compromised vascular microenvironment of T2DM is challenging and the factors that determine the adverse bone regeneration outcomes are poorly understood. For this purpose the effect of T2DM on osteogenic and angiogenic healing potential of calvarial bone, was evaluated in Zucker diabetic fatty (ZDF) rats, an established rat model for obese T2DM. MATERIALS AND METHODS: The study used 16-week-old ZDF rats and their age-matched controls, Zucker Lean (ZL)...
April 24, 2018: Bone
https://www.readbyqxmd.com/read/29701414/hilar-renal-artery-aneurysm-ex-vivo-reconstruction-and-autotransplantation
#17
Pedro Pinto Sousa, Carlos Veiga, Arlindo Matos, Pedro Sá Pinto, Rui Almeida
INTRODUCTION: Renal artery aneurysm (RAA) is a rare clinical entity with an estimated prevalence of 0.15% to 0.1%in the general population. The majority of patients present asymptomatically and the diagnosis is made incidentally during a hypertension study test, and more rarely, fortuitously after backache. Indications to treat have been subject of intense debate, nevertheless there seems to be some consensus that RAAs greater than 2 cm in diameter, expanding RAA, with thrombus or in pregnant women should be treated...
July 2017: Revista Portuguesa de Cirurgia Cardio-torácica e Vascular
https://www.readbyqxmd.com/read/29697462/ex-vivo-machine-perfusion-for-kidney-preservation
#18
Matyas Hamar, Markus Selzner
PURPOSE OF REVIEW: Machine perfusion is a novel strategy to decrease preservation injury, improve graft assessment, and increase organ acceptance for transplantation. This review summarizes the current advances in ex-vivo machine-based kidney preservation technologies over the last year. RECENT FINDINGS: Ex-vivo perfusion technologies, such as hypothermic and normothermic machine perfusion and controlled oxygenated rewarming, have gained high interest in the field of organ preservation...
April 24, 2018: Current Opinion in Organ Transplantation
https://www.readbyqxmd.com/read/29692722/the-deletion-of-endothelial-sodium-channel-%C3%AE-%C3%AE-enac-impairs-endothelium-dependent-vasodilation-and-endothelial-barrier-integrity-in-endotoxemia-in-vivo
#19
Magdalena Sternak, Anna Bar, Mateusz G Adamski, Tasnim Mohaissen, Brygida Marczyk, Anna Kieronska, Marta Stojak, Kamil Kus, Antoine Tarjus, Frederic Jaisser, Stefan Chlopicki
The role of epithelial sodium channel (ENaC) activity in the regulation of endothelial function is not clear. Here, we analyze the role of ENaC in the regulation of endothelium-dependent vasodilation and endothelial permeability in vivo in mice with conditional αENaC subunit gene inactivation in the endothelium (endo-αENaCKO mice) using unique MRI-based analysis of acetylcholine-, flow-mediated dilation and vascular permeability. Mice were challenged or not with lipopolysaccharide (LPS, from Salmonella typhosa , 10 mg/kg, i...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29689474/discovery-of-n-3-ethanimidoylamino-methyl-benzyl-l-prolinamide-dihydrochloride-a-new-potent-and-selective-inhibitor-of-the-inducible-nitric-oxide-synthase-as-a-promising-agent-for-the-therapy-of-malignant-glioma
#20
Cristina Maccallini, Mauro Di Matteo, Marialucia Gallorini, Monica Montagnani, Valentina Graziani, Alessandra Ammazzalorso, Pasquale Amoia, Barbara De Filippis, Sara Di Silvestre, Marialuigia Fantacuzzi, Letizia Giampietro, Maria A Potenza, Nazzareno Re, Assunta Pandolfi, Amelia Cataldi, Rosa Amoroso
In mammalian cells, aberrant iNOS induction may have detrimental consequences, and seems to be involved in the proliferation and progression of different tumors, such as malignant gliomas. Therefore, selective inhibition of iNOS could represent a feasible therapeutic strategy to treat these conditions. In this context, we have previously disclosed new acetamidines able to inhibit iNOS with a very high selectivity profile over eNOS or nNOS. Here we report the synthesis of a new series of compounds structurally related to the leading scaffold of N-[(3-aminomethyl)benzyl] acetamidine (1400 W), together with their in vitro activity and selectivity...
April 13, 2018: European Journal of Medicinal Chemistry
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