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Ex vivo perfusion

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https://www.readbyqxmd.com/read/28212881/current-status-and-future-aspects-of-ex-vivo-allograft-perfusion-for-heart-transplantation
#1
EDITORIAL
Norihide Fukushima
No abstract text is available yet for this article.
March 2017: Journal of Heart and Lung Transplantation
https://www.readbyqxmd.com/read/28202246/analysis-of-myocardial-perfusion-parameters-in-an-ex-vivo-porcine-heart-model-using-third-generation-dual-source-ct
#2
Gert Jan Pelgrim, Taylor M Duguay, J Marco A Stijnen, Akos Varga-Szemes, Sjoerd Van Tuijl, U Joseph Schoepf, Matthijs Oudkerk, Rozemarijn Vliegenthart
PURPOSE: To evaluate the relationship between fractional flow reserve (FFR)-determined coronary artery stenosis severity and myocardial perfusion parameters derived from dynamic myocardial CT perfusion imaging (CTP) in an ex-vivo porcine heart model. METHODS: Six porcine hearts were perfused according to Langendorff. Circulatory parameters such as arterial blood flow (ABF) (L/min), mean arterial pressure (MAP) (mmHg) and heart rate (bpm) were monitored. Using an inflatable cuff and monitored via a pressure wire, coronary artery stenoses of different FFR grades were created (no stenosis, FFR = 0...
February 3, 2017: Journal of Cardiovascular Computed Tomography
https://www.readbyqxmd.com/read/28199380/the-effects-of-pravastatin-on-the-normal-human-placenta-lessons-from-ex-vivo-models
#3
Adelina Balan, Irit Szaingurten-Solodkin, Shani S Swissa, Valeria Feinshtein, Mahmoud Huleihel, Gershon Holcberg, Doron Dukler, Ofer Beharier
INTRODUCTION: Research in animal models and preliminary clinical studies in humans support the use of pravastatin for the prevention of preeclampsia. However, its use during pregnancy is still controversial due to limited data about its effect on the human placenta and fetus. METHODS: In the present study, human placental cotyledons were perfused in the absence or presence of pravastatin in the maternal reservoir (PraM). In addition, placental explants were treated with pravastatin for 5, 24 and 72 h under normoxia and hypoxia...
2017: PloS One
https://www.readbyqxmd.com/read/28193783/remote-ischemic-preconditioning-triggers-changes-in-autonomic-nervous-system-activity-implications-for-cardioprotection
#4
Safa Abdul-Ghani, Arnold N Fleishman, Igor Khaliulin, Marco Meloni, Gianni D Angelini, M-Saadeh Suleiman
Cardioprotective efficacy of remote ischemic preconditioning (RIPC) remains controversial. Experimental studies investigating RIPC have largely monitored cardiovascular changes during index ischemia and reperfusion with little work investigating changes during RIPC application. This work aims to identify cardiovascular changes associated with autonomic nervous system (ANS) activity during RIPC and prior to index ischemia. RIPC was induced in anesthetized male C57/Bl6 mice by four cycles of 5 min of hindlimb ischemia using inflated cuff (200 mmHg) followed by 5 min reperfusion...
February 2017: Physiological Reports
https://www.readbyqxmd.com/read/28188872/pre-transplantation-31-p-magnetic-resonance-spectroscopy-for-quality-assessment-of-human-pancreatic-grafts-a-feasibility-study
#5
Lina Carlbom, Jan Weis, Lars Johansson, Olle Korsgren, Håkan Ahlström
OBJECTIVE: To investigate the feasibility of using (31)P-MRS for objective non-invasive quality assessment of human pancreas grafts prior to transplantation or islet isolation. MATERIALS AND METHODS: Pancreata from 5 human donors, 3 males and 2 females, aged 49-78years, with body mass index (BMI) 22-31kg/m(2), were included. Pancreata were perfused with histidine-tryptophan-ketoglutarate solution during procurement and stored in hypothermic condition (4°C) for 21-44h...
February 8, 2017: Magnetic Resonance Imaging
https://www.readbyqxmd.com/read/28177906/myocardial-protection-from-ischemia-reperfusion-injury-by-exogenous-galanin-fragment
#6
Andrei Timotin, Oleg Pisarenko, Maria Sidorova, Irina Studneva, Valentin Shulzhenko, Marina Palkeeva, Larisa Serebryakova, Aleksander Molokoedov, Oksana Veselova, Mathieu Cinato, Helene Tronchere, Frederic Boal, Oksana Kunduzova
BACKGROUND AND PURPOSE: Galanin is a multifunctional neuropeptide with pleiotropic roles. The present study was designed to evaluate the potential effects of galanin (2-11) (G1) on functional and metabolic abnormalities in response to myocardial ischemia-reperfusion (I/R) injury. EXPERIMENTAL APPROACH: Peptide G1 was synthesized by the 9-fluorenylmethoxycarbonyl (Fmoc)-based solid-phase method. The chemical structure was identified by 1H-NMR spectroscopy and mass spectrometry...
February 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/28169309/correlative-imaging-of-the-murine-hind-limb-vasculature-and-muscle-tissue-by-microct-and-light-microscopy
#7
Laura Schaad, Ruslan Hlushchuk, Sébastien Barré, Roberto Gianni-Barrera, David Haberthür, Andrea Banfi, Valentin Djonov
A detailed vascular visualization and adequate quantification is essential for the proper assessment of novel angiomodulating strategies. Here, we introduce an ex vivo micro-computed tomography (microCT)-based imaging approach for the 3D visualization of the entire vasculature down to the capillary level and rapid estimation of the vascular volume and vessel size distribution. After perfusion with μAngiofil®, a novel polymerizing contrast agent, low- and high-resolution scans (voxel side length: 2.58-0.66 μm) of the entire vasculature were acquired...
February 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28160138/pharmacodynamics-of-oxaliplatin-derived-platinum-compounds-during-hyperthermic-intraperitoneal-chemotherapy-hipec-an-emerging-aspect-supporting-the-rational-design-of-treatment-protocols
#8
Markus W Löffler, Heiko Schuster, Anne Zeck, Nicolas Quilitz, Jürgen Weinreich, Alexander Tolios, Sebastian P Haen, Philipp Horvath, Stefan Löb, Hans-Georg Rammensee, Ingmar Königsrainer, Alfred Königsrainer, Stefan Beckert
BACKGROUND: Hyperthermic intraperitoneal chemotherapy (HIPEC) is used to treat peritoneal surface malignancies with application of cytostatic drugs such as oxaliplatin (OX) after cytoreductive surgery. Despite its increased use, evidence for optimal drug dosage, and notably duration of HIPEC, is scarce. METHODS: In this study, OX distribution was comprehensively assessed in nine patients during HIPEC (300 mg OX/m(2) body surface area in Physioneal solution for 30 min)...
February 3, 2017: Annals of Surgical Oncology
https://www.readbyqxmd.com/read/28157238/resealable-optically-accessible-pdms-free-fluidic-platform-for-ex-vivo-interrogation-of-pancreatic-islets
#9
Giovanni Lenguito, Deborah Chaimov, Jonathan R Weitz, Rayner Rodriguez-Diaz, Siddarth A K Rawal, Alejandro Tamayo-Garcia, Alejandro Caicedo, Cherie L Stabler, Peter Buchwald, Ashutosh Agarwal
We report the design and fabrication of a robust fluidic platform built out of inert plastic materials and micromachined features that promote optimized convective fluid transport. The platform is tested for perfusion interrogation of rodent and human pancreatic islets, dynamic secretion of hormones, concomitant live-cell imaging, and optogenetic stimulation of genetically engineered islets. A coupled quantitative fluid dynamics computational model of glucose stimulated insulin secretion and fluid dynamics was first utilized to design device geometries that are optimal for complete perfusion of three-dimensional islets, effective collection of secreted insulin, and minimization of system volumes and associated delays...
February 3, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28135708/remote-ischaemic-preconditioning-and-sevoflurane-postconditioning-synergistically-protect-rats-from-myocardial-injury-induced-by-ischemia-and-reperfusion-partly-via-inhibition-tlr4-myd88-nf-%C3%AE%C2%BAb-signaling-pathway
#10
Jiru Zhang, Jing Zhang, Peng Yu, Min Chen, Qingfeng Peng, Zhiqiang Wang, Nan Dong
BACKGROUND/AIMS: A combination sevoflurane postconditioning (SPC) and remote ischemic preconditioning (RIPC) is proved effective in an ex vivo rat heart perfusion model. However, the combined effect of those two interventions is not tested in rat myocardial ischemia/reperfusion (I/R) model, and the underlying mechanisms remain to be elucidated. This study aimed to investigate the effect in vivo using a rat myocardial I/R model and illuminate the related mechanisms. METHODS: Forty male Sprague-Dawley rats were randomly divided into the following 5 groups: i) sham-operated control; ii) I/R; iii) I/R + RIPC; iv) I/R + SPC; v) I/R + RIPC + SPC...
January 16, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28132648/experimental-validation-of-contrast-enhanced-ssfp-cine-cmr-for-quantification-of-myocardium-at-risk-in-acute-myocardial-infarction
#11
David Nordlund, Mikael Kanski, Robert Jablonowski, Sasha Koul, David Erlinge, Marcus Carlsson, Henrik Engblom, Anthony H Aletras, Håkan Arheden
BACKGROUND: Accurate assessment of myocardium at risk (MaR) after acute myocardial infarction (AMI) is necessary when assessing myocardial salvage. Contrast-enhanced steady-state free precession (CE-SSFP) is a recently developed cardiovascular magnetic resonance (CMR) method for assessment of MaR up to 1 week after AMI. Our aim was to validate CE-SSFP for determination of MaR in an experimental porcine model using myocardial perfusion single-photon emission computed tomography (MPS) as a reference standard and to test the stability of MaR-quantification over time after injecting gadolinium-based contrast...
January 30, 2017: Journal of Cardiovascular Magnetic Resonance
https://www.readbyqxmd.com/read/28126891/2-18f-fluoroethanol-is-a-novel-positron-emission-tomography-pet-reporter-of-solid-tumour-perfusion
#12
Brennan J Wadsworth, Jinhe Pan, Iulia Dude, Nadine Colpo, Momir Bosiljcic, Kuo Shyan Lin, Francois Benard, Kevin L Bennewith
: Solid tumour perfusion is a proven variable of interest for predicting cancer aggression and response to therapy. Current methods for non-invasively imaging tumour perfusion with positron emission tomography (PET) are limited by restricted accessibility and short half-lives of perfusion radiotracers. This study presents 2-(18)F-fluoroethanol (2-(18)F-FEtOH) as a perfusion reporter that can distinguish between tumours of varying perfusion levels and can be applied to screening drugs that modify tumour perfusion...
January 26, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28126733/glycation-abolishes-the-cardioprotective-effects-of-albumin-during-ex%C3%A2-vivo-ischemia-reperfusion
#13
Rudo F Mapanga, Danzil E Joseph, Marco Saieva, Florence Boyer, Philippe Rondeau, Emmanuel Bourdon, M Faadiel Essop
Hyperglycemia-induced oxidative stress plays a key role in the onset/progression of cardiovascular diseases. For example, it can trigger formation of advanced glycation end (AGE) products with ischemia-reperfusion performed under hyperglycemic conditions. For this study, we hypothesized that albumin modified by glycation loses its unique cardioprotective properties in the setting of ischemia-reperfusion under high glucose conditions. Here, ex vivo rat heart perfusions were performed under simulated normo- and hyperglycemic conditions, that is Krebs-Henseleit buffer containing 11 mmol/L and 33 mmol/L glucose, respectively, ± normal or glycated albumin preparations...
January 2017: Physiological Reports
https://www.readbyqxmd.com/read/28115329/protocol-of-a-randomised-controlled-open-label-trial-of-ex-vivo-normothermic-perfusion-versus-static-cold-storage-in-donation-after-circulatory-death-renal-transplantation
#14
Sarah A Hosgood, Kourosh Saeb-Parsy, Colin Wilson, Christopher Callaghan, Dave Collett, Michael L Nicholson
INTRODUCTION: Ex vivo normothermic perfusion (EVNP) is a novel technique that reconditions the kidney and restores renal function prior to transplantation. Phase I data from a series of EVNP in extended criteria donor kidneys have established the safety and feasibility of the technique in clinical practice. METHODS AND ANALYSIS: This is a UK-based phase II multicentre randomised controlled trial to assess the efficacy of EVNP compared with the conventional static cold storage technique in donation after circulatory death (DCD) kidney transplantation...
January 23, 2017: BMJ Open
https://www.readbyqxmd.com/read/28104168/meld-and-child-pugh-scores-are-related-to-immune-status-of-intrahepatic-natural-killer-cells-in-liver-transplant-candidates
#15
N Tanimine, Y Tanaka, T Abe, J Piao, K Ishiyama, T Kobayashi, K Ide, M Ohira, H Tahara, S Shimizu, Y Saeki, H Sakai, T Yano, H Ohdan
BACKGROUND: The role and phenotypic alterations of intrahepatic natural killer (NK) cells in liver disease were investigated. Although intrahepatic NK cells reportedly functionally deteriorate in the fibrotic liver, it remains unclear how the clinical severity of liver disease affects intrahepatic NK cells in patients with advanced liver failure. METHODS: We analyzed the phenotypic properties of intrahepatic NK cells by using mononuclear cells extracted from ex vivo liver perfusate effluents from patients who underwent liver transplantation...
January 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28099049/effects-of-hydrogen-sulfide-releasing-compounds-on-aqueous-humor-outflow-facility-in-porcine-ocular-anterior-segments-ex-vivo
#16
Jenaye Robinson, Esther Okoro, Chinoso Ezuedu, Leah Bush, Catherine A Opere, Sunny E Ohia, Ya Fatou Njie-Mbye
PURPOSE: To investigate the pharmacological actions of hydrogen sulfide (H2S)-releasing compounds l-cysteine and sodium hydrosulfide (NaHS) on aqueous humor (AH) outflow facility in porcine ocular anterior segment. METHODS: Porcine ocular anterior segments were perfused with Dulbecco's modified Eagle's medium at a constant pressure of 7.35 mmHg. After stable outflow baseline, explants were exposed to NaHS or l-cysteine. The increase in outflow generated by the H2S-releasing compounds was measured in the absence and presence of inhibitor of H2S biosynthesis (aminooxyacetic acid; AOAA), blocker of KATP channels (glibenclamide), and inhibitor of adenylyl cyclase (SQ 22536)...
March 2017: Journal of Ocular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28096213/endothelial-cell-regulation-of-salivary-gland-epithelial-patterning
#17
Hae Ryong Kwon, Deirdre A Nelson, Kara A DeSantis, Jennifer M Morrissey, Melinda Larsen
Perfusion-independent regulation of epithelial pattern formation by the vasculature during organ development and regeneration is of considerable interest for application in restoring organ function. During murine submandibular salivary gland development, the vasculature co-develops with the epithelium during branching morphogenesis; however, it is not known whether the vasculature has instructive effects on the epithelium. Using pharmacological inhibitors and siRNA knockdown in embryonic organ explants, we determined that VEGFR2-dependent signaling is required for salivary gland epithelial patterning...
January 15, 2017: Development
https://www.readbyqxmd.com/read/28091727/intrinsic-cardiac-ganglia-and-acetylcholine-are-important-in-the-mechanism-of-ischaemic-preconditioning
#18
J M J Pickard, N Burke, S M Davidson, D M Yellon
This study aimed to investigate the role of the intrinsic cardiac nervous system in the mechanism of classical myocardial ischaemic preconditioning (IPC). Isolated perfused rat hearts were subjected to 35-min regional ischaemia and 60-min reperfusion. IPC was induced as three cycles of 5-min global ischaemia-reperfusion, and provided significant reduction in infarct size (IS/AAR = 14 ± 2% vs control IS/AAR = 48 ± 3%, p < 0.05). Treatment with the ganglionic antagonist, hexamethonium (50 μM), blocked IPC protection (IS/AAR = 37 ± 7%, p < 0...
March 2017: Basic Research in Cardiology
https://www.readbyqxmd.com/read/28087819/electrophysiologic-effects-of-the-ik1-inhibitor-pa-6-are-modulated-by-extracellular-potassium-in-isolated-guinea-pig-hearts
#19
Gregory S Hoeker, Mark A Skarsfeldt, Thomas Jespersen, Steven Poelzing
The pentamidine analog PA-6 was developed as a specific inward rectifier potassium current (IK1) antagonist, because established inhibitors either lack specificity or have side effects that prohibit their use in vivo. We previously demonstrated that BaCl2, an established IK1 inhibitor, could prolong action potential duration (APD) and increase cardiac conduction velocity (CV). However, few studies have addressed whether targeted IK1 inhibition similarly affects ventricular electrophysiology. The aim of this study was to determine the effects of PA-6 on cardiac repolarization and conduction in Langendorff-perfused guinea pig hearts...
January 2017: Physiological Reports
https://www.readbyqxmd.com/read/28079228/attenuated-recovery-of-contractile-function-in-aging-hearts-following-global-ischemia-reperfusion-role-of-extracellular-hsp27-and-tlr4
#20
Lihua Ao, Yufeng Zhai, Chunhua Jin, Joseph C Cleveland, David A Fullerton, Xianzhong Meng
BACKGROUND: While cardiac functional recovery is attenuated in the elderly following cardiac surgery with obligatory global myocardial ischemia/reperfusion (I/R), the underlying mechanism remains incompletely understood. We observed previously that human and mouse myocardium releases heat shock protein (HSP) 27 during global I/R. Extracellular HSP27 induces myocardial inflammatory response and plays a role in post-ischemic cardiac dysfunction in adult mouse hearts. OBJECTIVE: This study was to determine the role of extracellular HSP27 and Toll-like receptor 4 (TLR4) in the attenuated functional recovery in aging mouse hearts following global I/R...
December 19, 2016: Molecular Medicine
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