Yihao Zhang, Long Qian, Weijia Wei, Yu Wang, Beining Wang, Pingping Lin, Wenchao Liu, Luze Xu, Xiang Li, Dongming Liu, Sida Cheng, Jiaofeng Li, Yixuan Ye, Hang Li, Xiaohan Zhang, Yiming Dong, Xuejin Zhao, Cuihua Liu, Haoqian M Zhang, Qi Ouyang, Chunbo Lou
We developed an in vitro DNA detection system using a pair of dCas9 proteins linked to split halves of luciferase. Luminescence was induced upon co-localization of the reporter pair to a ~44 bp target sequence defined by sgRNAs. We used the system to detect Mycobacterium tuberculosis DNA with high specificity and sensitivity. The re-programmability of dCas9 was further leveraged in an array design that accesses sequence information across the entire genome.
October 10, 2016: ACS Synthetic Biology