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https://www.readbyqxmd.com/read/27027254/development-of-a-normothermic-extracorporeal-liver-perfusion-system-toward-improving-viability-and-function-of-human-extended-criteria-donor-livers
#1
Babak Banan, Rao Watson, Min Xu, Yiing Lin, William Chapman
Donor organ shortages have led to an increased interest in finding new approaches to recover organs from extended criteria donors (ECD). Normothermic extracorporeal liver perfusion (NELP) has been proposed as a superior preservation method to reduce ischemia/reperfusion injury (IRI), precondition suboptimal grafts, and treat ECD livers so that they can be successfully used for transplantation. The aim of this study was to investigate the beneficial effects of a modified NELP circuit on discarded human livers...
July 2016: Liver Transplantation
https://www.readbyqxmd.com/read/26865634/a-protein-derived-oxygen-is-the-source-of-the-amide-oxygen-of-nitrile-hydratases
#2
Micah T Nelp, Yang Song, Vicki H Wysocki, Vahe Bandarian
Nitrile hydratase metalloenzymes are unique and important biocatalysts that are used industrially to produce high value amides from their corresponding nitriles. After more than three decades since their discovery, the mechanism of this class of enzymes is becoming clear with evidence from multiple recent studies that the cysteine-derived sulfenato ligand of the active site metal serves as the nucleophile that initially attacks the nitrile. Herein we describe the first direct evidence from solution phase catalysis that the source of the product carboxamido oxygen is the protein...
April 8, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26780093/an-informatic-framework-for-decoding-protein-complexes-by-top-down-mass-spectrometry
#3
Owen S Skinner, Pierre C Havugimana, Nicole A Haverland, Luca Fornelli, Bryan P Early, Joseph B Greer, Ryan T Fellers, Kenneth R Durbin, Luis H F Do Vale, Rafael D Melani, Henrique S Seckler, Micah T Nelp, Mikhail E Belov, Stevan R Horning, Alexander A Makarov, Richard D LeDuc, Vahe Bandarian, Philip D Compton, Neil L Kelleher
Efforts to map the human protein interactome have resulted in information about thousands of multi-protein assemblies housed in public repositories, but the molecular characterization and stoichiometry of their protein subunits remains largely unknown. Here, we report a computational search strategy that supports hierarchical top-down analysis for precise identification and scoring of multi-proteoform complexes by native mass spectrometry.
March 2016: Nature Methods
https://www.readbyqxmd.com/read/26439190/novel-strategy-to-decrease-reperfusion-injuries-and-improve-function-of-cold-preserved-livers-using-normothermic-ex-vivo-liver-perfusion-machine
#4
Babak Banan, Zhenyu Xiao, Rao Watson, Min Xu, Jianluo Jia, Gundumi A Upadhya, Thalachallour Mohanakumar, Yiing Lin, William Chapman
Normothermic extracorporeal liver perfusion (NELP) can decrease ischemia/reperfusion injury to the greatest degree when cold ischemia time is minimized. Warm perfusion of cold-stored livers results in hepatocellular damage, sinusoidal endothelial cell (SEC) dysfunction, and Kupffer cell activation. However, the logistics of organ procurement mandates a period of cold preservation before NELP. The aim of this study was to determine the beneficial effects of gradual rewarming of cold-stored livers by placement on NELP...
March 2016: Liver Transplantation
https://www.readbyqxmd.com/read/26314201/delayed-resolution-of-syrinx-after-posterior-fossa-decompression-without-dural-opening-in-children-with-chiari-malformation-type-i
#5
Benjamin C Kennedy, Taylor B Nelp, Kathleen M Kelly, Michelle Q Phan, Samuel S Bruce, Michael M McDowell, Neil A Feldstein, Richard C E Anderson
OBJECT Chiari malformation Type I (CM-I) is associated with a syrinx in 25%-85% of patients. Although posterior fossa decompression (PFD) without dural opening is an accepted treatment option for children with symptomatic CM-I, many surgeons prefer to open the dura if a syrinx exists. The purpose of this study was to investigate the frequency and timing of syrinx resolution in children undergoing PFD without dural opening for CM-I. METHODS A retrospective review of 68 consecutive pediatric patients with CM-I and syringomyelia who underwent PFD without dural opening was conducted...
August 28, 2015: Journal of Neurosurgery. Pediatrics
https://www.readbyqxmd.com/read/26294088/normothermic-extracorporeal-liver-perfusion-for-donation-after-cardiac-death-dcd-livers
#6
COMPARATIVE STUDY
Babak Banan, Haniee Chung, Zhenyu Xiao, Yaman Tarabishy, Jianluo Jia, Pamela Manning, G A Upadhya, Thalachallour Mohanakumar, Yiing Lin, William C Chapman
BACKGROUND: The susceptibility of extended criteria livers to ischemia reperfusion injury is a major obstacle in organ cold preservation. Normothermic extracorporeal liver perfusion (NELP) has been investigated to reduce ischemic damage, restore physiologic function, and assess viability of the liver prior to transplant. The goal of this study is to compare physiological parameters of livers maintained continuously on NELP to those preserved in cold solution. METHODS: Livers from 9 female landrace pigs were subjected to either 20 minutes (W20-NELP), 40 minutes (W40-NELP), or 60 minutes (W60-NELP) of warm ischemia followed by 6 hours of NELP followed by a 2-hour NELP evaluation phase...
December 2015: Surgery
https://www.readbyqxmd.com/read/26228534/a-single-enzyme-transforms-a-carboxylic-acid-into-a-nitrile-through-an-amide-intermediate
#7
Micah T Nelp, Vahe Bandarian
The biosynthesis of nitriles is known to occur through specialized pathways involving multiple enzymes; however, in bacterial and archeal biosynthesis of 7-deazapurines, a single enzyme, ToyM, catalyzes the conversion of the carboxylic acid containing 7-carboxy-7-deazaguanine (CDG) into its corresponding nitrile, 7-cyano-7-deazaguanine (preQ0 ). The mechanism of this unusual direct transformation was shown to proceed via the adenylation of CDG, which activates it to form the newly discovered amide intermediate 7-amido-7-deazaguanine (ADG)...
September 1, 2015: Angewandte Chemie
https://www.readbyqxmd.com/read/26182039/193%C3%A2-delayed-resolution-of-syrinx-after-posterior-fossa-decompression-without-dural-opening-in-children-with-chiari-i-malformation
#8
Benjamin C Kennedy, Taylor B Nelp, Kathleen M Kelly, Michelle Q Phan, Samuel S Bruce, Michael Maurice McDowell, Neil Feldstein, Richard C E Anderson
INTRODUCTION: Chiari Malformation Type I (CM-I) is associated with syrinx in 25% to 85% of patients. Although posterior fossa decompression (PFD) without dural opening is an accepted treatment option for children with symptomatic CM-1, many surgeons prefer to open the dura if syrinx exists. The purpose of this study was to investigate the frequency and timing of syrinx resolution in children undergoing PFD without dural opening for CM-1. METHODS: A retrospective review was conducted of 68 consecutive pediatric patients with CM-I and syringomyelia who underwent PFD without dural opening...
August 2015: Neurosurgery
https://www.readbyqxmd.com/read/25492905/frontal-networks-associated-with-command-following-after-hemorrhagic-stroke
#9
Charles B Mikell, Garrett P Banks, Hans-Peter Frey, Brett E Youngerman, Taylor B Nelp, Patrick J Karas, Andrew K Chan, Henning U Voss, E Sander Connolly, Jan Claassen
BACKGROUND AND PURPOSE: Level of consciousness is frequently assessed by command-following ability in the clinical setting. However, it is unclear what brain circuits are needed to follow commands. We sought to determine what networks differentiate command following from noncommand following patients after hemorrhagic stroke. METHODS: Structural MRI, resting-state functional MRI, and electroencephalography were performed on 25 awake and unresponsive patients with acute intracerebral and subarachnoid hemorrhage...
January 2015: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/25406617/why-glioma-patients-seize-adding-more-pathological-gaba-to-the-glutamate-hypothesis
#10
Taylor B Nelp, Robert A McGovern, Guy M McKhann
No abstract text is available yet for this article.
December 2014: Neurosurgery
https://www.readbyqxmd.com/read/25161022/extended-normothermic-extracorporeal-perfusion-of-isolated-human-liver-after-warm-ischaemia-a-preliminary-report
#11
Rinaldo Bellomo, Bruno Marino, Graeme Starkey, Michael Fink, Bao Zhong Wang, Glenn M Eastwood, Leah Peck, Helen Young, Shane Houston, Alison Skene, Helen Opdam, Robert Jones
BACKGROUND: Donation after circulatory death (DCD) livers are at markedly increased risk of primary graft dysfunction and biliary tract ischaemia. Normothermic extracorporeal liver perfusion (NELP) may increase the ability to transplant DCD livers and may allow their use for artificial extracorporeal liver support of patients with fulminant liver failure. OBJECTIVE: We conducted two proof-of-concept experiments using human livers after DCD to assess the feasibility and functional efficacy of NELP over an extended period...
September 2014: Critical Care and Resuscitation: Journal of the Australasian Academy of Critical Care Medicine
https://www.readbyqxmd.com/read/24914472/the-alpha-subunit-of-nitrile-hydratase-is-sufficient-for-catalytic-activity-and-post-translational-modification
#12
Micah T Nelp, Andrei V Astashkin, Linda A Breci, Reid M McCarty, Vahe Bandarian
Nitrile hydratases (NHases) possess a mononuclear iron or cobalt cofactor whose coordination environment includes rare post-translationally oxidized cysteine sulfenic and sulfinic acid ligands. This cofactor is located in the α-subunit at the interfacial active site of the heterodimeric enzyme. Unlike canonical NHases, toyocamycin nitrile hydratase (TNHase) from Streptomyces rimosus is a unique three-subunit member of this family involved in the biosynthesis of pyrrolopyrimidine antibiotics. The subunits of TNHase are homologous to the α- and β-subunits of prototypical NHases...
June 24, 2014: Biochemistry
https://www.readbyqxmd.com/read/23931037/normothermic-extracorporeal-human-liver-perfusion-following-donation-after-cardiac-death
#13
Rinaldo Bellomo, Bruno Marino, Graham Starkey, Bhao Zhong Wang, Michael A Fink, Nan Zhu, Satoshi Suzuki, Shane Houston, Glenn Eastwood, Paolo Calzavacca, Neil Glassford, Brenton Chambers, Alison Skene, Antoine G Schneider, Daryl Jones, Andrew Hilton, Helen Opdam, Stephen Warrillow, Nicole Gauthier, Lynne Johnson, Robert Jones
Liver transplantation is a major life-saving procedure and donation after cardiac death (DCD) has increased the pool of potential liver donors. However, livers procured after DCD are at increased risk of primary graft dysfunction and biliary tract ischaemia. Normothermic extracorporeal liver perfusion (NELP) may increase the ability to protect, evaluate and, in future, transplant DCD livers. We conducted a proof-of-concept experiment using a human liver procured by DCD (deemed not suitable for liver donation) to assess the short-term (3 hours) feasibility, histological effects and functional efficacy of NELP...
June 2013: Critical Care and Resuscitation: Journal of the Australasian Academy of Critical Care Medicine
https://www.readbyqxmd.com/read/23795341/establishing-a-trauma-registry-in-bhutan-needs-and-process
#14
Stephen C Morris, Nicolas Manice, Taylor Nelp, Tashi Tenzin
BACKGROUND: Globally, trauma represents a growing and significant burden of disease. Many health systems have limited metrics with which to guide development and appropriately inform policy and management decisions with regard to trauma related health care delivery. FINDINGS: This paper outlines the establishment of need for improved trauma related metrics in the country of Bhutan and the process of development of a trauma registry at Jigme Dorji Wangchuck National Referral Hospital to meet that need...
December 2013: SpringerPlus
https://www.readbyqxmd.com/read/22963210/normothermic-extracorporeal-perfusion-of-isolated-porcine-liver-after-warm-ischaemia-a-preliminary-report
#15
Rinaldo Bellomo, Satoshi Suzuki, Bruno Marino, Graeme K Starkey, Brenton Chambers, Michael A Fink, Bao Zhong Wang, Shane Houston, Glenn Eastwood, Paolo Calzavacca, Neil Glassford, Alison Skene, Daryl A Jones, Robert Jones
Liver transplantation is a major life-saving procedure, and donation after cardiac death (DCD) has increased the pool of potential liver donors. However, DCD livers are at increased risk of primary graft dysfunction and biliary tract ischaemia. Normothermic extracorporeal liver perfusion (NELP) may increase the ability to protect, evaluate and, in future, transplant DCD livers. We conducted proof-of-concept experiments using a DCD model in the pig to assess the short-term (4 hours) feasibility and functional efficacy of NELP...
September 2012: Critical Care and Resuscitation: Journal of the Australasian Academy of Critical Care Medicine
https://www.readbyqxmd.com/read/19155970/recovery-of-warm-ischemic-rat-liver-grafts-by-normothermic-extracorporeal-perfusion
#16
Herman Tolboom, Roos E Pouw, Maria-Louisa Izamis, Jack M Milwid, Nripen Sharma, Alejandro Soto-Gutierrez, Yaakov Nahmias, Korkut Uygun, François Berthiaume, Martin L Yarmush
Liver transplantation is currently the only established treatment of end-stage liver disease, but it is limited by a severe shortage of viable donor livers. Donors after cardiac death (DCD) are an untapped source that could significantly increase the pool of available livers. Preservation of these DCD livers by conventional static cold storage (SCS) is associated with an unacceptable risk of primary nonfunction and delayed graft failure. Normothermic extracorporeal liver perfusion (NELP) has been suggested as an improvement over SCS...
January 27, 2009: Transplantation
https://www.readbyqxmd.com/read/18750740/single-photon-emission-computed-tomography-of-myocardial-perfusion-and-the-hepatic-blood-pool
#17
D H Lewis, W B Nelp
No abstract text is available yet for this article.
June 1990: Western Journal of Medicine
https://www.readbyqxmd.com/read/17596120/a-model-for-normothermic-preservation-of-the-rat-liver
#18
Herman Tolboom, Roos Pouw, Korkut Uygun, Yoko Tanimura, Maria-Louisa Izamis, François Berthiaume, Martin L Yarmush
Current techniques for the preservation of donor livers typically rely on cold temperatures (approximately 0-4 degrees C) to slow down metabolic processes. Recently, normothermic extracorporeal liver perfusion (NELP) has regained interest as a potentially promising approach for long-term liver preservation. Unlike cold-storage techniques, NELP attempts to maintain the liver in a near physiological environment, thus enabling normal metabolic and tissue repair processes to take place, which may help in the recovery of ischemically damaged and fatty donor livers, both of which represent significant untapped sources of donor livers...
August 2007: Tissue Engineering
https://www.readbyqxmd.com/read/16579753/evaluation-of-historical-factors-influencing-the-occurrence-and-distribution-of-mycobacterium-bovis-infection-among-wildlife-in-michigan
#19
Roseann Miller, John B Kaneene
OBJECTIVES: To determine historical events leading to establishment of bovine tuberculosis in the white-tailed deer population in the northeastern corner of the lower peninsula (NELP) of Michigan and describe factors relevant to the present outbreak of bovine tuberculosis in Michigan. SAMPLE POPULATION: Cattle and white-tailed deer in Michigan from 1920 to 1990. PROCEDURES: A search of extant historical documents (eg, scientific journals, books, public reports, and correspondence and internal reports from governmental agencies) was conducted...
April 2006: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/15686659/a-comparison-of-bile-composition-from-heart-beating-and-non-heart-beating-rabbit-organ-donors-during-normothermic-extracorporeal-liver-perfusion-experimental-evaluation-using-proton-magnetic-resonance-spectroscopy
#20
COMPARATIVE STUDY
M M Habib, T S Hafez, H G Parkes, A M Seifalian, B J Fuller, B R Davidson
BACKGROUND: Organs retrieved from marginal and non-heart-beating donors (NHBDs) have sustained variable degrees of preretrieval damage that result in an increased incidence of complications. Normothermic extracorporeal liver perfusion (NELP) provides an opportunity to evaluate and resuscitate such organs. The aim of this study was to identify markers of ischemic injury in bile during perfusion of livers from heart-beating donors (HBDs) and NHBDs. METHODS: Livers were retrieved from New Zealand white rabbits...
December 2004: Transplantation Proceedings
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