Yordan Nikolaev Georgiev, Ondrej Vasicek, Balik Dzhambazov, Tsvetelina Georgieva Batsalova, Petko Nedyalkov Denev, Lili Ivaylova Dobreva, Svetla Trifonova Danova, Svetlana Dimitrova Simova, Christian Winther Wold, Manol Hristov Ognyanov, Berit Smestad Paulsen, Albert Ivanov Krastanov
Macrolepiota procera (MP) is an edible mushroom used in the treatment of diabetes, hypertension and inflammation. However, the structure and biological effects of its polysaccharides (PSs) are unclear. This study investigates the structural features of a PS complex from MP (MP-PSC), its immunomodulatory activities and effects on probiotic and pathogenic bacteria. MP-PSC was obtained by boiling water, and PSs were characterized by 2D NMR spectroscopy. The immunomodulatory effects on blood and derived neutrophils, other leukocytes, and murine macrophages were studied by flow cytometry, chemiluminescence, spectrophotometry, and ELISA...
August 13, 2022: Journal of Fungi (Basel, Switzerland)