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Andrea Cipri, Christopher Schulz, Roland Ludwig, Lo Gorton, Manel Del Valle
A novel application of cellobiose dehydrogenase (CDH) as sensing element for a Bioelectronic Tongue (BioET) system has been tested. In this work CDHs from various fungi, which exhibit different substrate specificities, were used to discriminate between lactose and glucose in presence of the interfering matrix compound Ca(2+) in various mixtures. This work exploits the advantage of an electronic tongue system with practically zero pre-treatment of samples and operation at low voltages in a direct electron transfer mode...
May 15, 2016: Biosensors & Bioelectronics
Xavier Cetó, Francisco Céspedes, Manel del Valle
This work reports the application of a BioElectronic Tongue (BioET) in the estimation of polyphenol content in wine. The approach used an array of enzyme biosensors capable of giving a wide and complete response of the analyzed species, plus a chemometric processing tool able to interpret the chemical signals and extract meaningful data from the complex readings. In our case, the proposed BioET was formed by an array of four voltammetric enzymatic biosensors based on epoxy-graphite composites, one blank electrode and the other three bulk-modified with tyrosinase and laccase on one side, and copper nanoparticles on the other; these modifiers were used in order to incorporate differentiated or catalytic response to different polyphenols present in wine and aimed to the determination of its total polyphenol content value...
September 15, 2012: Talanta
Xavier Cetó, Francisco Céspedes, Maria Isabel Pividori, Juan Manuel Gutiérrez, Manel del Valle
This work reports the application of a Bio-Electronic Tongue (BioET) system made from an array of enzymatic biosensors in the analysis of polyphenols, focusing on major polyphenols found in wine. For this, the biosensor array was formed by a set of epoxy-graphite biosensors, bulk-modified with different redox enzymes (tyrosinase and laccase) and copper nanoparticles, aimed at the simultaneous determination of the different polyphenols. Departure information was the set of voltammograms generated with the biosensor array, selecting some characteristic features in order to reduce the data for the Artificial Neural Network (ANN)...
January 21, 2012: Analyst
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