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https://www.readbyqxmd.com/read/29724034/an-improved-weighted-partial-least-squares-method-coupled-with-near-infrared-spectroscopy-for-rapid-determination-of-multiple-components-and-anti-oxidant-activity-of-pu-erh-tea
#1
Ze Liu, Hua-Lin Xie, Lin Chen, Jian-Hua Huang
Background: Pu-erh tea is a unique microbially fermented tea, which distinctive chemical constituents and activities are worthy of systematic study. Near infrared spectroscopy (NIR) coupled with suitable chemometrics approaches can rapidly and accurately quantitatively analyze multiple compounds in samples. Methods: In this study, an improved weighted partial least squares (PLS) algorithm combined with near infrared spectroscopy (NIR) was used to construct a fast calibration model for determining four main components, i...
May 2, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29722013/effect-of-shading-intensity-on-morphological-and-color-traits-and-chemical-components-of-new-tea-camellia-sinensis-l-shoots-under-direct-covering-cultivation
#2
Tomohito Sano, Hideki Horie, Akiko Matsunaga, Yuhei Hirono
BACKGROUND: Use of covering cultivation to shade tea (Camellia sinensis L.) trees to produce high-quality, high-priced green tea has recently increased in Japan. Knowledge of shading effects on morphological and color traits, and chemical components of new tea shoots is important for product quality and productivity. We assessed these traits of tea shoots and their relationships under covering cultivation of various radiation intensities. RESULTS: Leaf thickness, LMA (leaf mass per area), and leaf density of new tea leaves were smaller under covering culture than under open-field culture...
May 2, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29678829/draft-genome-sequence-of-camellia-sinensis-var-sinensis-provides-insights-into-the-evolution-of-the-tea-genome-and-tea-quality
#3
Chaoling Wei, Hua Yang, Songbo Wang, Jian Zhao, Chun Liu, Liping Gao, Enhua Xia, Ying Lu, Yuling Tai, Guangbiao She, Jun Sun, Haisheng Cao, Wei Tong, Qiang Gao, Yeyun Li, Weiwei Deng, Xiaolan Jiang, Wenzhao Wang, Qi Chen, Shihua Zhang, Haijing Li, Junlan Wu, Ping Wang, Penghui Li, Chengying Shi, Fengya Zheng, Jianbo Jian, Bei Huang, Dai Shan, Mingming Shi, Congbing Fang, Yi Yue, Fangdong Li, Daxiang Li, Shu Wei, Bin Han, Changjun Jiang, Ye Yin, Tao Xia, Zhengzhu Zhang, Jeffrey L Bennetzen, Shancen Zhao, Xiaochun Wan
Tea, one of the world's most important beverage crops, provides numerous secondary metabolites that account for its rich taste and health benefits. Here we present a high-quality sequence of the genome of tea, Camellia sinensis var. sinensis (CSS), using both Illumina and PacBio sequencing technologies. At least 64% of the 3.1-Gb genome assembly consists of repetitive sequences, and the rest yields 33,932 high-confidence predictions of encoded proteins. Divergence between two major lineages, CSS and Camellia sinensis var...
April 20, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29669412/novel-flavoalkaloids-from-white-tea-with-inhibitory-activity-against-formation-of-advanced-glycation-end-products
#4
Xiao Li, Guang-Jin Liu, Wei Zhang, Yv-Long Zhou, Tie-Jun Ling, Xiao-Chun Wan, Guan-Hu Bao
Two novel flavoalkaloids, (-)-6-(5'''S)-N-ethyl-2-pyrrolidinone-epigallocatechin -O-gallate (ester-type catechins pyrrolidinone A, etc-pyrrolidinone A, 1), (-)-6-(5'''R)-N-ethyl-2-pyrrolidinone-epigallocatechin-O-gallate (etc-pyrrolidinone B,2) and new naturally occurring flavoalkaloids (-)-8-N-ethyl-2-pyrrolidinone-epigallocatechin-O-gallate (etc-pyrrolidinone C,3a and etc- pyrrolidinone D,3b), were isolated from white tea (Camellia sinensis). Their structures were identified by extensive NMR spectra...
April 18, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29662053/highly-stable-graphene-oxide-based-membranes-with-superior-permeability
#5
Khalid Hussain Thebo, Xitang Qian, Qing Zhang, Long Chen, Hui-Ming Cheng, Wencai Ren
Increasing fresh water demand for drinking and agriculture is one of the grand challenges of our age. Graphene oxide (GO) membranes have shown a great potential for desalination and water purification. However, it is challenging to further improve the water permeability without sacrificing the separation efficiency, and the GO membranes are easily delaminated in aqueous solutions within few hours. Here, we report a class of reduced GO membranes with enlarged interlayer distance fabricated by using theanine amino acid and tannic acid as reducing agent and cross-linker...
April 16, 2018: Nature Communications
https://www.readbyqxmd.com/read/29629712/l-theanine-protects-h9c2-cells-from-hydrogen-peroxide-induced-apoptosis-by-enhancing-antioxidant-capability
#6
Chengjian Li, Qiongxian Yan, Shaoxun Tang, Wenjun Xiao, Zhiliang Tan
BACKGROUND L-theanine is a non-protein amino acid in green tea, and its hepatoprotection and neuroprotection have been verified. However, whether L-theanine can prevent cardiomyocytes from apoptosis is unclear yet. This study evaluated the protective effects of L-theanine on H2O2-induced heart injury in vitro. MATERIAL AND METHODS The certified H9C2 cells were pretreated with L-theanine (0 mM, 4 mM, 8 mM, and 16 mM) for 24 h, followed by 160 µM H2O2 solution for 4 h. The cell viability and antioxidant indices were assayed...
April 9, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29629512/-l-theanine-decreases-orofacial-dyskinesia-induced-by-reserpine-in-rats
#7
Hung-Sheng Soung, Mao-Hsien Wang, Kuo-Chi Chang, Cheng-Neng Chen, Yi Chang, Chih-Chuan Yang, Hsiang-Chien Tseng
Reserpine (RES)-induced orofacial dyskinesia (OD) has been used as an animal model for human tardive dyskinesia (TD) for decades, due to its strong pathophysiological association with striatal oxidative stress and neural cytoarchitecture alteration. L -Theanine (LT), one of the major amino acid components in green tea, has potent antioxidative, anti-inflammatory, and neuroprotective effects. In this study, we examined the potential protective effects of LT on RES-induced behavioral and neurochemical dysfunction in rats...
April 9, 2018: Neurotoxicity Research
https://www.readbyqxmd.com/read/29580004/application-of-metabolomics-profiling-in-the-analysis-of-metabolites-and-taste-quality-in-different-subtypes-of-white-tea
#8
Chen Yang, Zhengyan Hu, Meiling Lu, Pengliang Li, Junfeng Tan, Mei Chen, Haipeng Lv, Yin Zhu, Yue Zhang, Li Guo, Qunhua Peng, Weidong Dai, Zhi Lin
Three subtypes of white tea, Silver Needle (SN), White Peony (WP), and Shou Mei (SM), differ in their taste, aroma, bioactivity, and commercial value. Here, a metabolomics investigation on the chemical compositions combining taste equivalent-quantification and dose-over-threshold (DoT) determination on the taste qualities were applied to comprehensively characterize the white tea subtypes for the first time. Significant differences in the contents of catechins, dimeric catechins, amino acids, phenolic acids, flavonol/flavone glycosides, and aroma precursors were observed among these 3 white teas...
April 2018: Food Research International
https://www.readbyqxmd.com/read/29568741/alteration-of-mevalonate-pathway-in-rat-splenic-lymphocytes-possible-role-in-cytokines-secretion-regulated-by-l-theanine
#9
Chengjian Li, Qiongxian Yan, Shaoxun Tang, Wenjun Xiao, Zhiliang Tan
L-Theanine is a nonprotein amino acid in tea, and its immunomodulatory function has been confirmed. This study aimed to investigate the effect of L-theanine addition on cytokines secretion in rat splenic lymphocytes and explore its potential immunomodulatory effects on the mevalonate biosynthetic pathway. Our results showed that L-theanine treatment did not influence the proliferation and division indexes of the splenic lymphocytes subsets. Interestingly, L-theanine treatment had regulated the contents of IFN- γ , IL-2, IL-4, IL-10, IL-12, and TNF- α   ( P < 0...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29518631/natural-compounds-analysis-using-liquid-and-supercritical-fluid-chromatography-hyphenated-to-mass-spectrometry-evaluation-of-a-new-design-of-atmospheric-pressure-ionization-source
#10
Oliver Ciclet, Denis Barron, Steve Bajic, Jean-Luc Veuthey, Davy Guillarme, Alexandre Grand-Guillaume Perrenoud
The MS hyphenation performance of UniSpray®, a new design of atmospheric pressure ionization source was evaluated in both SFC and RPLC modes. Sensitivity, stability, versatility and matrix effects offered by the UniSpray were assessed in positive and negative ionization modes and systematically compared to an electrospray source (ESI) using 120 natural compounds covering an extended chemical space. In a first instance, a multivariate approach was used to screen and optimize the UniSpray source settings to maximize detection sensitivity...
April 15, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29495349/neuroprotective-effects-and-mechanisms-of-tea-bioactive-components-in-neurodegenerative-diseases
#11
REVIEW
Shu-Qing Chen, Ze-Shi Wang, Yi-Xiao Ma, Wei Zhang, Jian-Liang Lu, Yue-Rong Liang, Xin-Qiang Zheng
As the population ages, neurodegenerative diseases such as Parkinson's disease (PD) and Alzheimer's disease (AD) impose a heavy burden on society and families. The pathogeneses of PD and AD are complex. There are no radical cures for the diseases, and existing therapeutic agents for PD and AD have diverse side effects. Tea contains many bioactive components such as polyphenols, theanine, caffeine, and theaflavins. Some investigations of epidemiology have demonstrated that drinking tea can decrease the risk of PD and AD...
February 25, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29487461/flavor-characteristics-and-chemical-compositions-of-oolong-tea-processed-using-different-semi-fermentation-times
#12
Pan-Pan Liu, Jun-Feng Yin, Gen-Sheng Chen, Fang Wang, Yong-Quan Xu
Flavor characteristics and chemical compositions of Tieguanyin oolong tea processed using different semi-fermentation times were investigated. Six flavor attributes of the teas, namely, astringency, bitterness, umami, sweet aftertaste, floral flavor, and green fruity flavor, were analysed. With extended semi-fermentation time, the taste intensity of sweet aftertaste increased, and the aroma intensity of floral and green fruity flavors increased, while the intensities of astringency, bitterness, and umami showed no clear trend...
March 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29482046/pharmacological-values-and-therapeutic-properties-of-black-tea-camellia-sinensis-a-comprehensive-overview
#13
REVIEW
Muhammad Naveed, Jannat BiBi, Asghar Ali Kamboh, Imran Suheryani, Ihsanullah Kakar, Sarfaraz Ali Fazlani, Xia FangFang, Shahmir Ali Kalhoro, Liang Yunjuan, Mohib Ullah Kakar, Mohamed E Abd El-Hack, Ahmed E Noreldin, Shi Zhixiang, Chen LiXia, Zhou XiaoHui
Medicinal plants are essential parts of traditional medicine due to their phytochemical constituents having pharmacological values and therapeutic applications. Black tea have thousands of various biological compounds such as flavonoids (Thearubigins (TRs) and theaflavins (TFs) and catechins), amino acids (L.theanine), vitamins (A, C, K), phenolic acids (caffeic acid (CA), gallic acid (GA), chlorogenic acids (CGA) and cauramic acid), lipids, proteins, volatile compounds carbohydrates, β-carotene and fluoride that illustrated many promising pharmacological effects regarded as growth promoter, cardioprotector, potent cholesterol-lowering effect, antioxidant and antimicrobial, etc inhuman...
April 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29462204/microbial-diversity-and-component-variation-in-xiaguan-tuo-tea-during-pile-fermentation
#14
Haizhou Li, Min Li, Xinrui Yang, Xin Gui, Guofeng Chen, Jiuyun Chu, Xingwang He, Weitao Wang, Feng Han, Ping Li
Xiaguan Tuo Tea is largely consumed by the Chinese, but there is little research into the microbial diversity and component changes during the fermentation of this tea. In this study, we first used fluorescence in situ hybridization (FISH), next-generation sequencing (NGS) and chemical analysis methods to determine the microbial abundance and diversity and the chemical composition during fermentation. The FISH results showed that the total number of microorganisms ranges from 2.3×102 to 4.0×108 cells per gram of sample during fermentation and is mainly dominated by fungi...
2018: PloS One
https://www.readbyqxmd.com/read/29458014/topical-delivery-of-l-theanine-ameliorates-tpa-induced-acute-skin-inflammation-via-downregulating-endothelial-pecam-1-and-neutrophil-infiltration-and-activation
#15
Wei-Jie Zeng, Zhi Tan, Xing-Fei Lai, Ya-Nan Xu, Chun-Lin Mai, Jun Zhang, Zhen-Jia Lin, Xian-Guo Liu, Shi-Li Sun, Li-Jun Zhou
l-theanine, the most abundant free amino acid in tea, has been documented to possess many different bioactive properties through oral or intragastrical delivery. However, little is known about the effect of topical delivery of l-theanine on acute inflammation. In the present study, by using 12-O-tetradecanoylphorbol-13-acetate (TPA, 2.5 μg/ear)-induced ear edema model in mice, we first found that single-dose local pretreatment of l-theanine 30 min before TPA time- and dose-dependently suppressed the increases in both skin thickness and weight...
March 25, 2018: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29420994/l-theanine-and-caffeine-improve-target-specific-attention-to-visual-stimuli-by-decreasing-mind-wandering-a-human-functional-magnetic-resonance-imaging-study
#16
Chanaka N Kahathuduwa, Chathurika S Dhanasekara, Shao-Hua Chin, Tyler Davis, Vajira S Weerasinghe, Tharaka L Dassanayake, Martin Binks
Oral intake of l-theanine and caffeine supplements is known to be associated with faster stimulus discrimination, possibly via improving attention to stimuli. We hypothesized that l-theanine and caffeine may be bringing about this beneficial effect by increasing attention-related neural resource allocation to target stimuli and decreasing deviation of neural resources to distractors. We used functional magnetic resonance imaging (fMRI) to test this hypothesis. Solutions of 200mg of l-theanine, 160mg of caffeine, their combination, or the vehicle (distilled water; placebo) were administered in a randomized 4-way crossover design to 9 healthy adult men...
January 2018: Nutrition Research
https://www.readbyqxmd.com/read/29401695/influence-of-graded-levels-of-l-theanine-dietary-supplementation-on-growth-performance-carcass-traits-meat-quality-organs-histomorphometry-blood-chemistry-and-immune-response-of-broiler-chickens
#17
Muhammad Saeed, Xu Yatao, Faiz-Ul Hassan, Muhammad Asif Arain, Mohamed E Abd El-Hack, Ahmed E Noreldin, Chao Sun
l-theanine is a water-soluble non-proteinous amino acid mainly found in green tea leaves. Despite the availability of abundant literature on green tea, studies on the use of l-theanine as a feed additive in animals, and especially broilers are limited. The objective of this study was, therefore, to evaluate the effect of different dietary levels of l-theanine on meat quality, growth performance, immune response, and blood metabolites in broilers. A total of 400 day-old broiler chicks were randomly divided into four treatment groups using a completely randomized design; C-control, basal diet; 100LT-basal diet + 100 mg l-theanine/kg diet; 200LT-basal diet + 200 mg l-theanine/kg diet; and 300LT-basal diet + 300 mg l-theanine/kg diet...
February 3, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29393264/prevention-of-doxorubicin-induced-renal-toxicity-by-theanine-in-rats
#18
Katsuhito Nagai, Shuhei Fukuno, Keisuke Otani, Yoshiki Nagamine, Sachiko Omotani, Yasutoshi Hatsuda, Michiaki Myotoku, Hiroki Konishi
Doxorubicin (DOX) is a highly potent anti-neoplastic agent widely used in clinical practice, but its dosage and duration of administration are strictly limited due to dose-related organ damage. In the present study, we examined whether theanine, an amino acid derivative found in green tea leaves, can protect against DOX-induced acute nephrotoxicity in rats. Decreases in the creatinine clearance by DOX administration were attenuated by concurrent treatment with theanine, which was consistent with the change in histological renal images assessed by microscopic examination...
2018: Pharmacology
https://www.readbyqxmd.com/read/29374957/protective-effect-of-l-theanine-on-haloperidol-induced-orofacial
#19
Cheng-Neng Chen, Kuo-Chi Chang, Mao-Hsien Wang, Hsiang-Chien Tseng, Hung-Sheng Soung
Tardive dyskinesia (TD) is a severe side effect of chronic neuroleptic treatment consisting of abnormal involuntary movements, characterized by orofacial dyskinesia (OD). Haloperidol (HAL)- induced OD has been widely used as an animal model to study the neuropathophysiology of human TD with its pathophysiology strongly associated with striatal oxidative stress. L-Theanine (LT), one of the major amino acid components in green tea, has potent antioxidative effects and is able to protect against various oxidative injuries...
February 28, 2018: Chinese Journal of Physiology
https://www.readbyqxmd.com/read/29364152/metabolite-profiling-of-14-wuyi-rock-tea-cultivars-using-uplc-qtof-ms-and-uplc-qqq-ms-combined-with-chemometrics
#20
Si Chen, Meihong Li, Gongyu Zheng, Tingting Wang, Jun Lin, Shanshan Wang, Xiaxia Wang, Qianlin Chao, Shixian Cao, Zhenbiao Yang, Xiaomin Yu
Wuyi Rock tea, well-recognized for rich flavor and long-lasting fragrance, is a premium subcategory of oolong tea mainly produced in Wuyi Mountain and nearby regions of China. The quality of tea is mainly determined by the chemical constituents in the tea leaves. However, this remains underexplored for Wuyi Rock tea cultivars. In this study, we investigated the leaf metabolite profiles of 14 major Wuyi Rock tea cultivars grown in the same producing region using UPLC-QTOF MS and UPLC-QqQ MS with data processing via principal component analysis and cluster analysis...
January 24, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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